vascular label fitc concanavalin a  (Vector Laboratories)


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    Name:
    Unconjugated Concanavalin A Con A
    Description:
    Concanavalin A Con A recognizes α linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins At neutral and alkaline pH Con A exists as a tetramer of four identical subunits below pH 5 6 Con A dissociates into active dimers of 52 kDa Acetylation succinylation or other derivatizations can also produce stable forms with dimeric structures See succinylated Con A Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds Con A requires calcium or manganese ions at each of its four saccharide binding sites Although these divalent metal ions are bound tightly to the polypeptide structure buffers which can bind calcium such as phosphate generally should be avoided in diluting Con A since a gradual loss in activity may occur Inhibiting Eluting Sugar mixture of 200 mM α methylmannoside 200 mM α methylglucoside
    Catalog Number:
    L-1000
    Price:
    None
    Category:
    Proteins
    Size:
    500 mg
    Buy from Supplier


    Structured Review

    Vector Laboratories vascular label fitc concanavalin a
    Unconjugated Concanavalin A Con A
    Concanavalin A Con A recognizes α linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins At neutral and alkaline pH Con A exists as a tetramer of four identical subunits below pH 5 6 Con A dissociates into active dimers of 52 kDa Acetylation succinylation or other derivatizations can also produce stable forms with dimeric structures See succinylated Con A Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds Con A requires calcium or manganese ions at each of its four saccharide binding sites Although these divalent metal ions are bound tightly to the polypeptide structure buffers which can bind calcium such as phosphate generally should be avoided in diluting Con A since a gradual loss in activity may occur Inhibiting Eluting Sugar mixture of 200 mM α methylmannoside 200 mM α methylglucoside
    https://www.bioz.com/result/vascular label fitc concanavalin a/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vascular label fitc concanavalin a - by Bioz Stars, 2021-04
    95/100 stars

    Images

    1) Product Images from "Pharmacologic Uncoupling of Angiogenesis and Inflammation during Initiation of Pathological Corneal Neovascularization *"

    Article Title: Pharmacologic Uncoupling of Angiogenesis and Inflammation during Initiation of Pathological Corneal Neovascularization *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M111.294967

    Epithelial debridement induces rapid and consistent corneal angiogenesis and inflammation by 3 days. A , representative FITC-concanavalin A-injected corneal flat mounts at various times over 20 days following epithelial debridement. B , quantification of total vascular area per cornea normalized to control (undebrided) limbal vessel areas ( n = 10 corneas/group). Error bars indicate S.E. NV Area , neovascular area. C , H E-stained corneal sections showing the progression of re-epithelialization over a matter of days. The images are all oriented with the limbus to the left ( L ) and the central cornea to the right ( C ). Note the repair epithelium at the wound edge ( arrowhead ) accompanied by underlying inflammatory infiltrates. By day 7, the cornea completely resurfaced with a stratified epithelium ( E ) but includes the presence of new shallow stromal vessels ( S ) ( arrows ). The presence of inflammatory cells was reduced by this time (20×).
    Figure Legend Snippet: Epithelial debridement induces rapid and consistent corneal angiogenesis and inflammation by 3 days. A , representative FITC-concanavalin A-injected corneal flat mounts at various times over 20 days following epithelial debridement. B , quantification of total vascular area per cornea normalized to control (undebrided) limbal vessel areas ( n = 10 corneas/group). Error bars indicate S.E. NV Area , neovascular area. C , H E-stained corneal sections showing the progression of re-epithelialization over a matter of days. The images are all oriented with the limbus to the left ( L ) and the central cornea to the right ( C ). Note the repair epithelium at the wound edge ( arrowhead ) accompanied by underlying inflammatory infiltrates. By day 7, the cornea completely resurfaced with a stratified epithelium ( E ) but includes the presence of new shallow stromal vessels ( S ) ( arrows ). The presence of inflammatory cells was reduced by this time (20×).

    Techniques Used: Injection, Staining

    Related Articles

    Whole Genome Amplification:

    Article Title: Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
    Article Snippet: .. Lectins included concanavalin A (ConA), Galanthus nivalis lectin (GNL), peanut agglutinin (PNA), and wheat germ agglutinin (WGA), and were purchased from Vector Laboratories, Inc. (Burlingame, California, USA). ..

    Incubation:

    Article Title: The Metabolic Chemical Reporter 6‑Azido-6-deoxy-glucose Further Reveals the Substrate Promiscuity of O‑GlcNAc Transferase and Catalyzes the Discovery of Intracellular Protein Modification by O‑Glucose
    Article Snippet: .. The blots washed three times in TBST for 5 min and incubated with biotin-conjugated Concanavalin A (Vector Lab), diluted 1:1000 in TBST, for 1 h. The blot was then washed 3× with TBST for 10 min. Then the blot was then incubated with Strep-HRP at 1:1000 in blocking buffer for 1 h. After being washed 3× with TBST for 10 min, the blot was developed using ECL reagents. .. BL21(DE3) chemically competent E. coli (Novagen) were transformed with a pET24b plasmid encoding 6His-tagged ncOGT (nucleocytoplasmic OGT) by heat shock and plated on selective LB agar plates containing 50 μ g mL−1 kanamycin (LB-kan).

    Article Title: Glycan Profiling Shows Unvaried N-Glycomes in MSC Clones with Distinct Differentiation Potentials
    Article Snippet: .. Following 15 min incubation on ice, 120 μL of 10 μg/mL FITC-ConA (Vector Labs) was added to each sample. .. After two 15 min incubations interspersed with flicking of the tubes, 1 mL of washing buffer was added and the samples were centrifuged for 5 min at 450 g. The cell pellet was resuspended in 100 μL of washing buffer containing 1 μg/mL DAPI and incubated on ice in the dark for 5 min, followed by the addition of 1 mL washing buffer, and centrifugation for 5 min at 450 g. The pellet was resuspended in 400 μL PBS for flow cytometry.

    Blocking Assay:

    Article Title: The Metabolic Chemical Reporter 6‑Azido-6-deoxy-glucose Further Reveals the Substrate Promiscuity of O‑GlcNAc Transferase and Catalyzes the Discovery of Intracellular Protein Modification by O‑Glucose
    Article Snippet: .. The blots washed three times in TBST for 5 min and incubated with biotin-conjugated Concanavalin A (Vector Lab), diluted 1:1000 in TBST, for 1 h. The blot was then washed 3× with TBST for 10 min. Then the blot was then incubated with Strep-HRP at 1:1000 in blocking buffer for 1 h. After being washed 3× with TBST for 10 min, the blot was developed using ECL reagents. .. BL21(DE3) chemically competent E. coli (Novagen) were transformed with a pET24b plasmid encoding 6His-tagged ncOGT (nucleocytoplasmic OGT) by heat shock and plated on selective LB agar plates containing 50 μ g mL−1 kanamycin (LB-kan).

    Staining:

    Article Title: Ischaemia-induced retinal neovascularisation and diabetic retinopathy in mice with conditional knockout of hypoxia-inducible factor-1 in retinal Müller cells
    Article Snippet: Briefly, mice were perfused through the left ventricle to remove circulating leucocytes in blood vessels. .. The adherent leucocytes in the vasculature were stained by perfusion with FITC-conjugated concanavalin-A (40 μg/ml; Vector Laboratories) and counted. .. The quantitative data were analysed and compared with those from wild-type mice using unpaired Student’s t test (two-tailed test).

    Labeling:

    Article Title: Arginase as a Mediator of Diabetic Retinopathy
    Article Snippet: The images were analyzed for reaction intensity by using the MetaMorph Image System (Molecular Devices). .. Leukocyte adhesion Retinal leukostasis was assayed by labeling the adherent leukocytes using Concanavalin A (Vector Laboratories). .. TUNEL assay Endothelial cell death was studied using TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) assay using Fluorescein in situ cell death detection kit (Millipore) according to the manufacturer’s protocol.

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    Vector Laboratories vascular label fitc concanavalin a
    Epithelial debridement induces rapid and consistent corneal angiogenesis and inflammation by 3 days. A , representative <t>FITC-concanavalin</t> A-injected corneal flat mounts at various times over 20 days following epithelial debridement. B , quantification of total vascular area per cornea normalized to control (undebrided) limbal vessel areas ( n = 10 corneas/group). Error bars indicate S.E. NV Area , neovascular area. C , H E-stained corneal sections showing the progression of re-epithelialization over a matter of days. The images are all oriented with the limbus to the left ( L ) and the central cornea to the right ( C ). Note the repair epithelium at the wound edge ( arrowhead ) accompanied by underlying inflammatory infiltrates. By day 7, the cornea completely resurfaced with a stratified epithelium ( E ) but includes the presence of new shallow stromal vessels ( S ) ( arrows ). The presence of inflammatory cells was reduced by this time (20×).
    Vascular Label Fitc Concanavalin A, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular label fitc concanavalin a/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vascular label fitc concanavalin a - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Epithelial debridement induces rapid and consistent corneal angiogenesis and inflammation by 3 days. A , representative FITC-concanavalin A-injected corneal flat mounts at various times over 20 days following epithelial debridement. B , quantification of total vascular area per cornea normalized to control (undebrided) limbal vessel areas ( n = 10 corneas/group). Error bars indicate S.E. NV Area , neovascular area. C , H E-stained corneal sections showing the progression of re-epithelialization over a matter of days. The images are all oriented with the limbus to the left ( L ) and the central cornea to the right ( C ). Note the repair epithelium at the wound edge ( arrowhead ) accompanied by underlying inflammatory infiltrates. By day 7, the cornea completely resurfaced with a stratified epithelium ( E ) but includes the presence of new shallow stromal vessels ( S ) ( arrows ). The presence of inflammatory cells was reduced by this time (20×).

    Journal: The Journal of Biological Chemistry

    Article Title: Pharmacologic Uncoupling of Angiogenesis and Inflammation during Initiation of Pathological Corneal Neovascularization *

    doi: 10.1074/jbc.M111.294967

    Figure Lengend Snippet: Epithelial debridement induces rapid and consistent corneal angiogenesis and inflammation by 3 days. A , representative FITC-concanavalin A-injected corneal flat mounts at various times over 20 days following epithelial debridement. B , quantification of total vascular area per cornea normalized to control (undebrided) limbal vessel areas ( n = 10 corneas/group). Error bars indicate S.E. NV Area , neovascular area. C , H E-stained corneal sections showing the progression of re-epithelialization over a matter of days. The images are all oriented with the limbus to the left ( L ) and the central cornea to the right ( C ). Note the repair epithelium at the wound edge ( arrowhead ) accompanied by underlying inflammatory infiltrates. By day 7, the cornea completely resurfaced with a stratified epithelium ( E ) but includes the presence of new shallow stromal vessels ( S ) ( arrows ). The presence of inflammatory cells was reduced by this time (20×).

    Article Snippet: Prior to euthanasia, all animals were injected with 100 μl of the vascular label FITC-concanavalin A (Vector Laboratories, Burlingame, CA) intravenously.

    Techniques: Injection, Staining