vascular endothelial growth factor vegf (Thermo Fisher)

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Vascular Endothelial Growth Factor VEGF Ab 7
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ms-1467-pcl
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1) Product Images from "Biological and Demographic Profile of Meningiomas in a Cohort of Egyptian Patients: Impact on Tumor Recurrence"
Article Title: Biological and Demographic Profile of Meningiomas in a Cohort of Egyptian Patients: Impact on Tumor Recurrence
Journal: The Scientific World Journal
doi: 10.1155/2013/375139

Figure Legend Snippet: (a) EMA immunostain highlighting whorls of meningioma cells (200x); (b) intense and diffuse VEGF expression in an anaplastic meningioma (200x); (c) Ki67 immunostain revealing a moderate proliferative activity in a case of atypical meningioma (200x); (d) benign meningioma with significant PR expression (200x); (e) CD20 highlighting rare B lymphocytes (100x); (f) CD3 showing a large population of T lymphocytes (100x).
Techniques Used: Expressing, Activity Assay
2) Product Images from "Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress"
Article Title: Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress
Journal: Stem Cells International
doi: 10.1155/2018/9682856

Figure Legend Snippet: Early passage ASCs overexpress the neurotropic protein HGF but not VEGF nor the proinflammatory cytokine IL-1 β . ASCs at passage 3 that were cultured in serum-free conditions for 48 hours were compared to the control group of ASCs at passage 5. Both cells and medium were collected and analyzed at mRNA level and at protein level by qRT-PCR and by ELISA, respectively. (a) qRT-PCR analysis of HGF, VEGF, and IL-1 β . (b) ELISA for HGF protein levels. Each experiment was performed a minimum of 3 samples from 3 different patients. Each experiment was performed a minimum of 3 times.
Techniques Used: Cell Culture, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay
3) Product Images from "Exosome Delivered Anticancer Drugs Across the Blood-Brain Barrier for Brain Cancer Therapy in Danio Rerio"
Article Title: Exosome Delivered Anticancer Drugs Across the Blood-Brain Barrier for Brain Cancer Therapy in Danio Rerio
Journal: Pharmaceutical research
doi: 10.1007/s11095-014-1593-y

Figure Legend Snippet: Inhibitory of VEGF in vivo using bEND.3 exosome delivered doxorubicin on zebrafish cancer model (* Results are significantly different, p
Techniques Used: In Vivo
4) Product Images from "Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress"
Article Title: Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress
Journal: Stem Cells International
doi: 10.1155/2018/9682856

Figure Legend Snippet: Early passage ASCs overexpress the neurotropic protein HGF but not VEGF nor the proinflammatory cytokine IL-1 β . ASCs at passage 3 that were cultured in serum-free conditions for 48 hours were compared to the control group of ASCs at passage 5. Both cells and medium were collected and analyzed at mRNA level and at protein level by qRT-PCR and by ELISA, respectively. (a) qRT-PCR analysis of HGF, VEGF, and IL-1 β . (b) ELISA for HGF protein levels. Each experiment was performed a minimum of 3 samples from 3 different patients. Each experiment was performed a minimum of 3 times.
Techniques Used: Cell Culture, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay
5) Product Images from "Conditioned media from endothelial progenitor cells cultured in simulated microgravity promote angiogenesis and bone fracture healing"
Article Title: Conditioned media from endothelial progenitor cells cultured in simulated microgravity promote angiogenesis and bone fracture healing
Journal: Stem Cell Research & Therapy
doi: 10.1186/s13287-020-02074-y

Figure Legend Snippet: MG-CM facilitated neovascularization of fracture areas in vivo. a Microfil perfusion was performed to examine the newly formed vessels of the regenerated areas followed by micro-CT scan. b Total vessel volume was calculated and analyzed based on reconstructive vessel images. c – h Immunohistochemistry staining was employed to detect the expression of CD31, VEGF-A, and MMP-9 in regenerative areas followed by quantitative analyses. Abbreviations: NG-CM, conditioned media from endothelial progenitor cells under normal gravity; MG-CM, conditioned media from endothelial progenitor cells under microgravity; VEGF-A, vascular endothelial growth factor A; MMP-9, matrix metalloproteinase 9. Data were presented as the mean ± standard deviation. * P
Techniques Used: In Vivo, Micro-CT, Immunohistochemistry, Staining, Expressing, Standard Deviation

Figure Legend Snippet: MG suppressed EPCs’ proliferation but activated HIF-1α/eNOS/NO axis. a MTT assay was performed to detect EPCs’ proliferation under MG or NG exposure. b Immunofluorescence staining showed the expression levels of Ki67 under NG (left) or MG (right) exposure. DAPI was for nuclear counterstain. c A cluster of angiogenic genes expression was examined by qRT-PCR after 12-, 24- and 48-h exposures of NG and MG, and GAPDH was used as the internal reference. d Western blot was employed to identify the expression of HIF-1α, eNOS and iNOS at protein levels. e Griess assay was performed to detect the NO contents in MG-CM and NG-CM after 12-, 24-, and 48-h exposures, and the concentrations were normalized to cell numbers. This experiment was repeated independently three times. Abbreviations: EPCs, endothelial progenitor cells; MG, microgravity; NG, normal gravity; DAPI, 4, 6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HIF-1α, hypoxia-induced factor-1α; eNOS, endothelial nitric oxide synthase; iNOS, inducible nitric oxide synthase; NO, nitric oxide; VEGF, vascular endothelial growth factor; MMP-9, matrix metalloproteinase 9; PDGF-B, platelet-derived growth factor-B; Ang-2, angiogenin-2. Data were presented as the mean ± standard deviation. * P
Techniques Used: MTT Assay, Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Western Blot, Griess Assay, Derivative Assay, Standard Deviation

Figure Legend Snippet: NO-induced activation of FAK/Erk1/2-MAPK signaling pathway contributed to enhanced pro-angiogenic properties of MG-CM. a Western blot was employed to detect the phosphorylation levels of FAK and Erk1/2. b The phosphorylation level of Erk1/2 was examined after Erk1/2-MAPK-selective inhibitor PD98059 application for 4 h. c , d Tube formation assay was performed to detect the angiogenic abilities of HUVECs when PD98059 was added or not, followed by quantitative analysis. e Immunofluorescence staining results showed the expression of Ki67 and CD31 in different groups. DAPI was for nuclear counterstain. f The mRNA expression level of VEGF was detected by qRT-PCR, and GAPDH was used as internal reference. g The schematic illustration of MG-induced HIF-1α/eNOS/NO activation of EPCs promoting HUVECs’ proliferation, migration, and angiogenesis. This experiment was repeated independently three times. Abbreviations: EPCs, endothelial progenitor cells; HUVECs, human umbilical vein endothelial cells; FAK, focal adhesion kinase; NG-CM, conditioned media from endothelial progenitor cells under normal gravity; MG-CM, conditioned media from endothelial progenitor cells under microgravity; NO, nitric oxide; DAPI, 4, 6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-induced factor-1α; eNOS, endothelial nitric oxide synthase. Data were presented as the mean ± standard deviation. * P
Techniques Used: Activation Assay, Western Blot, Tube Formation Assay, Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Migration, Standard Deviation

Figure Legend Snippet: MG-CM promoted HUVECs’ proliferation, migration, and angiogenesis in vitro partially through increased NO production. a The effects of DMEM, NG-CM, MG-CM, and NO reduced MG-CM on HUVECs’ proliferation were analyzed by MTT assay. b The Ki67 expression in HUVECs was detected by immunofluorescence staining. DAPI was for nuclear counterstain. c – f Transwell assay and wound healing assay were employed to detect the migration capacity changes of HUVECs after treatment followed by quantitative analyses. g The CD31 expression levels of HUVECs in each group were detected by immunofluorescence staining. h , i Tube formation assay was used to detect the angiogenic abilities of HUVECs followed by quantitative analysis. j qRT-PCR results showed the relative expression levels of VEGF and MMP-9, with GAPDH as the internal reference. This experiment was repeated independently three times. Abbreviations: HUVECs, human umbilical vein endothelial cells; NG-CM, conditioned media from endothelial progenitor cells under normal gravity; MG-CM, conditioned media from endothelial progenitor cells under microgravity; NO, nitric oxide; DAPI, 4, 6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VEGF, vascular endothelial growth factor; MMP-9, matrix metalloproteinase 9. Data were presented as the mean ± standard deviation. * P
Techniques Used: Migration, In Vitro, MTT Assay, Expressing, Immunofluorescence, Staining, Transwell Assay, Wound Healing Assay, Tube Formation Assay, Quantitative RT-PCR, Standard Deviation
6) Product Images from "Xanthohumol Impairs Human Prostate Cancer Cell Growth and Invasion and Diminishes the Incidence and Progression of Advanced Tumors in TRAMP Mice"
Article Title: Xanthohumol Impairs Human Prostate Cancer Cell Growth and Invasion and Diminishes the Incidence and Progression of Advanced Tumors in TRAMP Mice
Journal: Molecular Medicine
doi: 10.2119/molmed.2012.00174

Figure Legend Snippet: XN inhibits FAK, AKT and NF-κB activation and controls VEGF release and the migratory potential of PCa cells. Western blot analyses show a remarkable decrease of constitutive FAK phosphorylation after 5 h of treatment with XN (A), which correlates
Techniques Used: Activation Assay, Western Blot
7) Product Images from "Inducible Costimulator (ICOS) and ICOS Ligand Signaling Has Pivotal Roles in Skin Wound Healing via Cytokine Production"
Article Title: Inducible Costimulator (ICOS) and ICOS Ligand Signaling Has Pivotal Roles in Skin Wound Healing via Cytokine Production
Journal: The American Journal of Pathology
doi: 10.1016/j.ajpath.2011.07.048

Figure Legend Snippet: Cytokines, chemokines, and growth factors in wounds from wild-type (WT) and mutant mice. A: Expression of IL-4, IL-6, IL-10, TNF-α, CTGF, PDGF, TGF-β, and VEGF mRNAs measured by quantitative RT-PCR. B: Concentrations of IL-6, IL-10, TNF-α,
Techniques Used: Mutagenesis, Mouse Assay, Expressing, Quantitative RT-PCR
8) Product Images from "CYR61, a product of a growth factor-inducible immediate early gene, promotes angiogenesis and tumor growth"
Article Title: CYR61, a product of a growth factor-inducible immediate early gene, promotes angiogenesis and tumor growth
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi:

Figure Legend Snippet: Chemotactic activity of CYR61. The migration of HMVECs was measured in a modified Boyden chamber assay. The cells placed in the lower chamber that migrated into the upper chamber were counted in three high power fields for each condition after a 4-hr incubation at 37°C. CYR61 was used at 1 μg/ml (except for dose response in A ), vitronectin was used at 5 μg/ml, bFGF was used at 10 ng/ml, and VEGF was used at 1 ng/ml as chemoattractants. ( A ) CYR61-stimulated cell migration is dose-dependent. Cells that migrated into the upper well, where the indicated amount of purified CYR61 (▪) or the equivalent amount of CYR61 storage buffer (○) was placed, were counted. In parallel, HMVEC migration was measured by using bFGF as a chemoattractant, placed in the upper well with various amounts of CYR61 storage buffer corresponding to the amounts used in CYR61-stimulated migration experiment. Cells migrated are expressed as a percentage of bFGF-induced migration ± SD ( B ) Specific inhibition of CYR61-stimulated cell migration by anti-Cyr61 antibodies (25 μg/ml). HMVEC migration was monitored as described above by using either CYR61 or bFGF as a chemoattractant. Where indicated, these proteins were preincubated with anti-Cyr61 antibodies before use. ( C ) Directed migration of HMVEC tested in a checkerboard-type analysis. CYR61 or bFGF was added to the upper chamber, the lower chamber, neither chamber, or both chambers as indicated. In B and C , results are shown as the number of cells that migrated per three high power fields ± SD ( D ) Specific inhibition of CYR61-stimulated migration by an antibody against integrin α V β 3 but not α V β 5 . HMVEC migration was monitored by using CYR61, vitronectin, bFGF, or VEGF as chemoattractants. Where indicated, cells were preincubated with 50 μg/ml LM609 for 1 hr before addition to the lower chamber. Results are expressed as percentage of cells migrated to VEGF. Neg., background migration in the absence of chemoattractant.
Techniques Used: Activity Assay, Migration, Modification, Boyden Chamber Assay, Incubation, Purification, Inhibition
9) Product Images from "Nano-biological mesh constructed by astragaloside-IV-induced bone marrow mesenchymal stem cells on PLGA-NPs-SIS can be used for abdominal wall reconstruction"
Article Title: Nano-biological mesh constructed by astragaloside-IV-induced bone marrow mesenchymal stem cells on PLGA-NPs-SIS can be used for abdominal wall reconstruction
Journal: American Journal of Translational Research
doi:

Figure Legend Snippet: Protein and mRNA expression of inflammatory and angiogenic factors. A. qRT-PCR analysis of COL1A1, COL3A1, VEGF, MMP-9, MCP-1, and IL-6 levels; relative mRNA levels are based on GAPDH expression. B. WB analysis of the expression of indicated proteins (GAPDH was adopted as a loading control). *P
Techniques Used: Expressing, Quantitative RT-PCR, Western Blot
10) Product Images from "Metabolic Syndrome Triggered by Fructose Diet Impairs Neuronal Function and Vascular Integrity in ApoE-KO Mouse Retinas: Implications of Autophagy Deficient Activation"
Article Title: Metabolic Syndrome Triggered by Fructose Diet Impairs Neuronal Function and Vascular Integrity in ApoE-KO Mouse Retinas: Implications of Autophagy Deficient Activation
Journal: Frontiers in Cell and Developmental Biology
doi: 10.3389/fcell.2020.573987

Figure Legend Snippet: Vascular analysis. (A) Representative photomicrographs (scale of 100 μm, 100x magnification) of GSA-IB4 labeled (green) blood vessels in flat-mounts of the central area of whole retinas in the different experimental groups at 4 months of diet (n = 3). (B) Scheme of quantification (top left panel), bars represent the average vascular density (% area, bottom left panel), the average vascular diameter (μm, top right panel) and the average vascular branching (bottom right panel) of flat-mounted whole retinas of mice from the different experimental groups at 4 months of diet. (C) representative photomicrographs (scale 100 μm, 50x magnification) of α-SMA labeled (red) blood vessels in flat-mounts of the central area of whole retinas of mice from the different experimental groups at 4 months of diet ( n = 4). Bars represent the average vascular density (% area). (D) Bars represent the average VEGF (top panel) and HIF-1α (bottom panel) mRNA levels relative to actin in mouse retinal extracts of the different experimental groups at 4 months of diet ( n = 4). (E) Representative photomicrographs (scale 500 μm, 100x magnification) of albumin–Evans blue-complex leakage (red) in flat-mounts of the whole retinas in mice from the different experimental groups at 4 months of diet ( n = 3). Square indicate the place of 1000x magnification, scale 250 μm. (F) Bars represent albumin protein relative to actin levels in retinal extracts of mice from the different experimental groups at 4 months of diet (bottom panel, n = 3). Representative blot is showed (top panel). (G) representative photomicrographs (scale of 100 μm, 50x magnification) of GFAP labeled (red) in flat-mounted of central area of the whole retinas of mice from the different experimental groups at 4 months of diet ( n = 3). Squares indicate the place of 500x magnification, scale 500 μm. (H) Bars represent average protein expression of GFAP ( n = 4) and GS ( n = 5) relative to tubulin in retinal extracts of mice from the different experimental groups at 4 months of diet (bottom panel). Representative blot is showed (top panel). (I) Representative photomicrographs (scale 25 μm, 200x magnification) of GFAP label (green) in retinal criosections (10 μm) of mice from the different experimental groups at 4 months of diet ( n = 3). Data correspond to mean ± SEM. Two-way ANOVA followed by Bonferroni post hoc test or Kruskal–Wallis followed by Dunn’s post hoc test. * p
Techniques Used: Labeling, Mouse Assay, Expressing
11) Product Images from "Effects of amniotic membrane transplantation on cytokines expression in chemically burned rat corneas"
Article Title: Effects of amniotic membrane transplantation on cytokines expression in chemically burned rat corneas
Journal: International Journal of Ophthalmology
doi: 10.3980/j.issn.2222-3959.2011.01.07

Figure Legend Snippet: Effects of AMT on the expressions of EGF, bFGF, VEGF and MPP-2 in corneas of rats
Techniques Used:
12) Product Images from "Candesartan targeting of angiotensin II type 1 receptor demonstrates benefits for hypertension in pregnancy via the NF-κB signaling pathway"
Article Title: Candesartan targeting of angiotensin II type 1 receptor demonstrates benefits for hypertension in pregnancy via the NF-κB signaling pathway
Journal: Molecular Medicine Reports
doi: 10.3892/mmr.2018.9070

Figure Legend Snippet: Effects of Candesartan on Ang-IITR and expression levels of hypertension-related proteins in vascular endothelial cells. (A) Angiotensin expression levels in control, healthy or Candesartan-treated vascular endothelial cells. (B) Angiotensin release in vascular endothelial cells after treatment with homocysteine or Candesartan. (C) Ang-IITR expression levels and (D) angiotensin plasma concentration levels in control or Candesartan-treated mice with gestational hypertension. Analysis of (E) VEGF, (F) TGF-β, (G) Ang-1 and (H) PLGF expression levels in control, healthy or Candesartan-treated vascular endothelial cells. The data are presented as the mean ± standard error. *P
Techniques Used: Expressing, Concentration Assay, Mouse Assay
13) Product Images from "Follistatin‐Like 3 Enhances the Function of Endothelial Cells Derived from Pluripotent Stem Cells by Facilitating β‐Catenin Nuclear Translocation Through Inhibition of Glycogen Synthase Kinase‐3β Activity"
Article Title: Follistatin‐Like 3 Enhances the Function of Endothelial Cells Derived from Pluripotent Stem Cells by Facilitating β‐Catenin Nuclear Translocation Through Inhibition of Glycogen Synthase Kinase‐3β Activity
Journal: Stem Cells (Dayton, Ohio)
doi: 10.1002/stem.2820
![... growth factor (FGF), and vascular endothelial growth factor (VEGF) for 5 days. The differentiated cells were seeded ... Induced pluripotent stem cell differentiation toward endothelial cells. Human iPS Cells were differentiated using StemPro serum free media supplemented with BMP4, Activin A, fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF) for 5 days. The differentiated cells were seeded on collagen IV, while CD144 positive cells were magnetically sorted on day 6 using MicroBeads Kit (Miltenyi BIotec) and culturing in EGM‐2 media (LONZA). (A) : Images show morphology of iPS Cells (left panel) and of their differentiated EC counterparts (right panel) Scale bar: 50 μm. (B) : Real time polymerase chain reaction (PCR) data revealed significantly increased early and (C) late EC marker mRNA expression (Data is means ±SEM [ n = 3], *, p](https://storage.googleapis.com/bioz_article_images/PMC6099345/STEM-36-1033-g001.jpg)
Figure Legend Snippet: Induced pluripotent stem cell differentiation toward endothelial cells. Human iPS Cells were differentiated using StemPro serum free media supplemented with BMP4, Activin A, fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF) for 5 days. The differentiated cells were seeded on collagen IV, while CD144 positive cells were magnetically sorted on day 6 using MicroBeads Kit (Miltenyi BIotec) and culturing in EGM‐2 media (LONZA). (A) : Images show morphology of iPS Cells (left panel) and of their differentiated EC counterparts (right panel) Scale bar: 50 μm. (B) : Real time polymerase chain reaction (PCR) data revealed significantly increased early and (C) late EC marker mRNA expression (Data is means ±SEM [ n = 3], *, p
Techniques Used: Cell Differentiation, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Marker, Expressing
14) Product Images from "Macrophage Migration Inhibitory Factor Is Involved in Ectopic Endometrial Tissue Growth and Peritoneal-Endometrial Tissue Interaction In Vivo: A Plausible Link to Endometriosis Development"
Article Title: Macrophage Migration Inhibitory Factor Is Involved in Ectopic Endometrial Tissue Growth and Peritoneal-Endometrial Tissue Interaction In Vivo: A Plausible Link to Endometriosis Development
Journal: PLoS ONE
doi: 10.1371/journal.pone.0110434

Figure Legend Snippet: Real-time PCR analysis of the expression of genes mainly involved in cell adhesion and vascularisation of endometriosis-like lesions. Histogram representation of the effect of MIF genetic depletion or antagonism versus controls on VEGF (A, B), COX2 (C, D), BCL2 (E, F), BAX (G, H), ITGAV (I, J) and ITGB3 (K, L) mRNA expression in endometriosis-like lesions by quantitative real time PCR. For each factor, the ratio of mRNA level to GAPDH mRNA was determined. Results were from WT and KOmice (n = 6) with no treatment (controls) and from WT treated with ISO-1 (n = 5). Data are mean ± SEM; *, p
Techniques Used: Real-time Polymerase Chain Reaction, Expressing
15) Product Images from "Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure"
Article Title: Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure
Journal: Journal of Immunology Research
doi: 10.1155/2016/9151607

Figure Legend Snippet: mRNA gene expression of MCP-1, IL-1, NOS3, TGF- β , TNF- α , and VEGF in the kidney of control offspring and diabetic offspring with or without folic acid, aged two months. (a) The gene expression of MCP-1 was increased in the CFA2 group; (b and c) the gene expression of IL-1 and NOS3 showed no significant difference between the study groups; (d) the gene expression of TGF- β was decreased in the DC2 group; (e and f) the gene expression of TNF- α and VEGF showed no significant difference between the groups. ∗ Significant differences.
Techniques Used: Expressing

Figure Legend Snippet: mRNA gene expression of MCP-1, IL-1, NOS3, TGF- β , TNF- α , and VEGF in the kidney of control offspring and diabetic offspring with or without folic acid, aged five months. (a, b, c, d, and e) The gene expression of MCP-1, IL-1, NOS3, TGF- β , and TNF- α showed no significant difference between the study groups; (f) the gene expression of VEGF was decreased in the CFA5 and DFA5 groups. ∗ Significant differences.
Techniques Used: Expressing
16) Product Images from "A clinical and basic study of optimal endometrial preparation protocols for patients with infertility undergoing frozen-thawed embryo transfer"
Article Title: A clinical and basic study of optimal endometrial preparation protocols for patients with infertility undergoing frozen-thawed embryo transfer
Journal: Experimental and Therapeutic Medicine
doi: 10.3892/etm.2020.8914

Figure Legend Snippet: Quantitative analysis of mean endometrial LIF, integrin β3, OPN, VEGF, HOXA10and HOXA11mRNA levels of patients who did not undergo embryo transfers on the day of embryo transfer. * P
Techniques Used:
17) Product Images from "The Sulfamate Small Molecule CAIX Inhibitor S4 Modulates Doxorubicin Efficacy"
Article Title: The Sulfamate Small Molecule CAIX Inhibitor S4 Modulates Doxorubicin Efficacy
Journal: PLoS ONE
doi: 10.1371/journal.pone.0161040

Figure Legend Snippet: Effect of S4 on doxorubicin efficacy in HT29 –CAIX high and HT29 –CAIX low cells. Exposing HT29 cells to doxycycline (doxy) reduced CA9 mRNA levels during normoxia (N) and hypoxia (H), whereas VEGF levels were unaffected ( A ). CAIX protein levels minimized in HT29 –CAIX low cells when exposed to doxycycline ( B ). Quantification of three independent biological repeats showed minimal residual CAIX protein expression in HT29 –CAIX low cells as compared to parental cells or HT29 –CAIX high cells without a KD ( C ). Cell viability assays of HT29 –CAIX high ( D ) or HT29 –CAIX low cells ( E ) with increasing concentrations of doxorubicin. Cells were exposed to vehicle (black) or S4 (green) during normoxia (N), or to vehicle (red) or S4 (blue) during hypoxia (H). Results of three independent biological repeats are shown (mean ± SEM).
Techniques Used: Expressing
18) Product Images from "Role of LAMA4 Gene in Regulating Extravillous Trophoblasts in Pathogenesis of Preeclampsia"
Article Title: Role of LAMA4 Gene in Regulating Extravillous Trophoblasts in Pathogenesis of Preeclampsia
Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
doi: 10.12659/MSM.917402

Figure Legend Snippet: VEGF and sFlt-1 mRNA levels. ( A ) RT-PCR band, ( B ) VEGF and sFlt-1 mRNA levels. * p
Techniques Used: Reverse Transcription Polymerase Chain Reaction
19) Product Images from "Hair growth-promoting effect of Geranium sibiricum extract in human dermal papilla cells and C57BL/6 mice"
Article Title: Hair growth-promoting effect of Geranium sibiricum extract in human dermal papilla cells and C57BL/6 mice
Journal: BMC Complementary and Alternative Medicine
doi: 10.1186/s12906-017-1624-4

Figure Legend Snippet: Hair growth-stimulating effect of GSE in an in vivo model. a Observation of C57BL/6 mice hair growth for 3 weeks. b Histological analysis of hair follicles, mast cells, and stem cell factor expression on the back skin of C57BL/6 mice. c The numbers of mast cells were counted by toluidine blue staining. d Expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and transforming growth factor beta 1 (TGF-β1) in the back skin of C57BL/6 mice. The fold changes were normalized to the expression of glyceraldehyde 3-phosphate dehydrogenase; the values are expressed as the mean ± SEM. Values sharing the same superscript letters differ significantly at p
Techniques Used: In Vivo, Mouse Assay, Expressing, Staining

Figure Legend Snippet: Changes in the levels of Ki67 protein, hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta 1 (TGF-β1) in human dermal papilla cells (hDPCs) induced by GSE. a Immunohistochemistry of Ki67. b Numbers of Ki67-positive cells. c Relative expression levels of HGF, VEGF, and TGF-β1 in GSE-treated hDPCs. The fold changes were normalized to the expression of glyceraldehyde 3-phosphate dehydrogenase; the values are expressed as the mean ± SEM. Values sharing the same superscript letters differ significantly at p
Techniques Used: Immunohistochemistry, Expressing
20) Product Images from "Topographical cues regulate the crosstalk between MSCs and macrophages"
Article Title: Topographical cues regulate the crosstalk between MSCs and macrophages
Journal: Biomaterials
doi: 10.1016/j.biomaterials.2014.10.028

Figure Legend Snippet: Overview of the effect of topographical cues in the soluble factor-guided communication between MSCs and macrophages. MSCs were seeded in 2D substrates (left panel) or 3D scaffolds (right panel) and co-cultured with macrophages using a transwell insert system. The figure illustrates soluble factors whose levels decreased (red), increased (green) or were not affected (blue) in 3D co-cultures as compared to 2D settings. 3D topography sensing activates MSCs to produce PGE 2 and TSG-6 anti-inflammatory proteins while attenuates the secretion of IL-6, MCP-1, RANTES, GM-CSF, M-CSF and RANKL in co-cultures with macrophages. TNF-α, IL-10 and VEGF levels are unaffected by topographical features of the substrate. The lower number of monocytes in 3D settings represents the decrease in monocyte migration to the local inflammatory milieu provided by 3D-arranged MSCs as compared to 2D co-cultures. This attenuation in monocyte recruitment is mediated, at least in part, by reduced levels of IL-6 and MCP-1 (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).
Techniques Used: Cell Culture, Migration
21) Product Images from "Intracoronary Administration of Allogeneic Adipose Tissue–Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial Infarction"
Article Title: Intracoronary Administration of Allogeneic Adipose Tissue–Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial Infarction
Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
doi: 10.1161/JAHA.117.005771

Figure Legend Snippet: Effects of ATMSC s therapy on myocardial vascularization and perfusion. A, Microphotographs on the left (endothelial cells stained in brown and smooth muscle cells stained in red) and bar chart on the right showing increased vessel density in the border infarct zone of animals receiving cell therapy. B, Bar chart on the left and cardiac magnetic resonance images (myocardial quantitative perfusion maps) on the right illustrate an enhancement on myocardial perfusion of infarct border zone in animals receiving allogeneic ATMSC s. Arrows point to perfusion analysis region of interest (gray, anterior infarct border; white, core infarcted area; black, septum infarct border; yellow, posterior remote myocardium). C, Charts show up‐regulation of the pro‐angiogenic mediators SDF ‐1α, GM ‐ CSF , and VEGF in the infarcted tissue of the ATMSC s group compared with vehicle group, as assessed by qPCR . * P
Techniques Used: Staining, Real-time Polymerase Chain Reaction
22) Product Images from "Differences in peritoneal response after exposure to low-GDP bicarbonate/lactate-buffered dialysis solution compared to conventional dialysis solution in a uremic mouse model"
Article Title: Differences in peritoneal response after exposure to low-GDP bicarbonate/lactate-buffered dialysis solution compared to conventional dialysis solution in a uremic mouse model
Journal: International Urology and Nephrology
doi: 10.1007/s11255-018-1872-3

Figure Legend Snippet: Exposure to a bicarbonate/lactate-buffered solution is associated with increase of pro-inflammatory cytokines. Protein levels of the main pro-inflammatory cytokines detected in effluents collected from mouse after 8 weeks of PD fluid exposure. Cytokines levels (ρg/ml) are represented as means ± SD ( n ≥ 7) as follows: a TGFβ1, b IL-1β, c IL-6, d TNFα, e VEGF, f INFγ, g IL-17, h IL-4, i IL-5, j MIP-1α, k MIP-1β. C control, LS lactate PD fluid, BLS bicarbonate/lactate PD fluid. Differences were considered statistically significant for P
Techniques Used:
23) Product Images from "Differences in peritoneal response after exposure to low-GDP bicarbonate/lactate-buffered dialysis solution compared to conventional dialysis solution in a uremic mouse model"
Article Title: Differences in peritoneal response after exposure to low-GDP bicarbonate/lactate-buffered dialysis solution compared to conventional dialysis solution in a uremic mouse model
Journal: International Urology and Nephrology
doi: 10.1007/s11255-018-1872-3

Figure Legend Snippet: Exposure to a bicarbonate/lactate-buffered solution is associated with increase of pro-inflammatory cytokines. Protein levels of the main pro-inflammatory cytokines detected in effluents collected from mouse after 8 weeks of PD fluid exposure. Cytokines levels (ρg/ml) are represented as means ± SD ( n ≥ 7) as follows: a TGFβ1, b IL-1β, c IL-6, d TNFα, e VEGF, f INFγ, g IL-17, h IL-4, i IL-5, j MIP-1α, k MIP-1β. C control, LS lactate PD fluid, BLS bicarbonate/lactate PD fluid. Differences were considered statistically significant for P
Techniques Used:
24) Product Images from "Addition of Mesenchymal Stem Cells Enhances the Therapeutic Effects of Skeletal Myoblast Cell-Sheet Transplantation in a Rat Ischemic Cardiomyopathy Model"
Article Title: Addition of Mesenchymal Stem Cells Enhances the Therapeutic Effects of Skeletal Myoblast Cell-Sheet Transplantation in a Rat Ischemic Cardiomyopathy Model
Journal: Tissue Engineering. Part A
doi: 10.1089/ten.tea.2012.0534

Figure Legend Snippet: Expression of VEGF and HGF is higher at the infarct area. (A, B) Levels of mRNA in the transplanted infarct and infarct-remote heart areas by real-time PCR using rat-specific primers. The HGF and VEGF mRNA expressions within the transplanted infarct area
Techniques Used: Expressing, Real-time Polymerase Chain Reaction
25) Product Images from "Boswellic Acid Inhibits Growth and Metastasis of Human Colorectal Cancer in Orthotopic Mouse Model By Downregulating Inflammatory, Proliferative, Invasive, and Angiogenic Biomarkers"
Article Title: Boswellic Acid Inhibits Growth and Metastasis of Human Colorectal Cancer in Orthotopic Mouse Model By Downregulating Inflammatory, Proliferative, Invasive, and Angiogenic Biomarkers
Journal: International Journal of Cancer. Journal International du Cancer
doi: 10.1002/ijc.26251

Figure Legend Snippet: AKBA inhibits the expression of NF-κB and NF-κB-regulated gene products in CRC samples. (A) Electrophoretic mobility shift assay analysis showed the inhibition of NF-κB by AKBA in nuclear extracts from animal tissue. (B) Western blot showing that AKBA inhibited the expression of NF-κB-dependent gene products that regulate proliferation (cyclin D1 and COX-2), invasion (ICAM-1 and MMP-9), metastasis (CXCR4), and angiogenesis (VEGF). AKBA inhibits the expression of antiapoptotic gene products such as IAP-1, Bcl-xL, Bcl-2, and survivin in CRC tissues. Samples from three animals in each group were analyzed, and representative data are shown. V, vehicle; L, low (AKBA 50 mg/kg); M, medium (100 mg/kg); and H, high (200 mg/kg).
Techniques Used: Expressing, Electrophoretic Mobility Shift Assay, Inhibition, Western Blot
26) Product Images from "Follistatin‐Like 3 Enhances the Function of Endothelial Cells Derived from Pluripotent Stem Cells by Facilitating β‐Catenin Nuclear Translocation Through Inhibition of Glycogen Synthase Kinase‐3β Activity"
Article Title: Follistatin‐Like 3 Enhances the Function of Endothelial Cells Derived from Pluripotent Stem Cells by Facilitating β‐Catenin Nuclear Translocation Through Inhibition of Glycogen Synthase Kinase‐3β Activity
Journal: Stem Cells (Dayton, Ohio)
doi: 10.1002/stem.2820
![... growth factor (FGF), and vascular endothelial growth factor (VEGF) for 5 days. The differentiated cells were seeded ... Induced pluripotent stem cell differentiation toward endothelial cells. Human iPS Cells were differentiated using StemPro serum free media supplemented with BMP4, Activin A, fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF) for 5 days. The differentiated cells were seeded on collagen IV, while CD144 positive cells were magnetically sorted on day 6 using MicroBeads Kit (Miltenyi BIotec) and culturing in EGM‐2 media (LONZA). (A) : Images show morphology of iPS Cells (left panel) and of their differentiated EC counterparts (right panel) Scale bar: 50 μm. (B) : Real time polymerase chain reaction (PCR) data revealed significantly increased early and (C) late EC marker mRNA expression (Data is means ±SEM [ n = 3], *, p](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099345/bin/STEM-36-1033-g001.jpg)
Figure Legend Snippet: Induced pluripotent stem cell differentiation toward endothelial cells. Human iPS Cells were differentiated using StemPro serum free media supplemented with BMP4, Activin A, fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF) for 5 days. The differentiated cells were seeded on collagen IV, while CD144 positive cells were magnetically sorted on day 6 using MicroBeads Kit (Miltenyi BIotec) and culturing in EGM‐2 media (LONZA). (A) : Images show morphology of iPS Cells (left panel) and of their differentiated EC counterparts (right panel) Scale bar: 50 μm. (B) : Real time polymerase chain reaction (PCR) data revealed significantly increased early and (C) late EC marker mRNA expression (Data is means ±SEM [ n = 3], *, p
Techniques Used: Cell Differentiation, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Marker, Expressing
27) Product Images from "Per2 Mutation Recapitulates the Vascular Phenotype of Diabetes in the Retina and Bone Marrow"
Article Title: Per2 Mutation Recapitulates the Vascular Phenotype of Diabetes in the Retina and Bone Marrow
Journal: Diabetes
doi: 10.2337/db12-0172

Figure Legend Snippet: Decrease in VEGF receptor expression and upregulation of TGF-β-induced profibrotic gene expression in Per2 mutant retinas. There was no significant change in VEGFR1 ( A ) or VEGFR2 ( B ) expression in retinas of 4-month-old Per2 mutant mice; however, there was a dramatic decrease in mRNA expression of both receptor expressions in 12-month-old animals. VEGF expression remained unchanged in both groups ( C ). Per2 mutant retinas showed a profound increase in mRNA expression of TGF-β1 ( D ) and its downstream effectors PAI-1 ( E ), ID-1 ( F ), CTGF ( G ), and FN ( H ). The most prevalent change in mRNA expression was observed in 4-month-old Per2 mutant mice for TGF-β1, PAI-1, and FN, whereas ID-1 expression was significantly increased in 12-month-old Per2 mutant mice. CTGF mRNA was increased in both age groups. n = 7 for 4-month-old mice; n = 3 for 12-month-old mice; white bars, wild type; black bars, Per2 mutant.
Techniques Used: Expressing, Mutagenesis, Mouse Assay
28) Product Images from "Enhanced expression of mRNA for nuclear factor ?B1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis"
Article Title: Enhanced expression of mRNA for nuclear factor ?B1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis
Journal: Arthritis Research & Therapy
doi: 10.1186/ar1915

Figure Legend Snippet: Suppression of the production of matrix metalloproteinase (MMP)-1 and vascular endothelial growth factor (VEGF) by silencing nuclear factor (NF)κB1 mRNA in bone marrow CD34+ cells from patients with rheumatoid arthritis. Purified bone marrow CD34+ cells from 12 patients with rheumatoid arthritis were transfected with small interfering RNA (siRNA) for NFκB1 or a scrambled sequence control siRNA, after which the cells were further incubated in culture medium with stem cell factor (10 ng/ml), granulocyte-macrophage colony stimulating factor (1 ng/ml) and tumor necrosis factor-α (10 ng/ml) for 4 weeks with no medium changes. After the incubation, the supernatants were harvested and assayed for MMP-1 and VEGF by ELISA. Statistical significance was evaluated by Wilcoxon's signed rank test.
Techniques Used: Purification, Transfection, Small Interfering RNA, Sequencing, Incubation, Enzyme-linked Immunosorbent Assay

Figure Legend Snippet: Suppression of the production of matrix metalloproteinase (MMP)-1 and vascular endothelial growth factor (VEGF) by silencing nuclear factor (NF)κB1 mRNA in bone marrow CD34+ cells from patients with rheumatoid arthritis. Purified bone marrow CD34+ cells from 12 patients with rheumatoid arthritis were transfected with small interfering RNA (siRNA) for NFκB1 or a scrambled sequence control siRNA, after which the cells were further incubated in culture medium with stem cell factor (10 ng/ml), granulocyte-macrophage colony stimulating factor (1 ng/ml) and tumor necrosis factor-α (10 ng/ml) for 4 weeks with no medium changes. After the incubation, the supernatants were harvested and assayed for MMP-1, VEGF and β 2 -microglobulin (β 2 MG) by ELISA. Statistical significance was evaluated by Wilcoxon's signed rank test.
Techniques Used: Purification, Transfection, Small Interfering RNA, Sequencing, Incubation, Enzyme-linked Immunosorbent Assay
29) Product Images from "Cultured Human Epidermis Combined With Meshed Skin Autografts Accelerates Epithelialization and Granulation Tissue Formation in a Rat Model"
Article Title: Cultured Human Epidermis Combined With Meshed Skin Autografts Accelerates Epithelialization and Granulation Tissue Formation in a Rat Model
Journal: Annals of Plastic Surgery
doi: 10.1097/SAP.0000000000001058

Figure Legend Snippet: Growth factor release by hCE. The release of bFGF, IL-1α, IL-1β, IL-6, PDGF-AA, TGF-α, TGF-β1, VEGF, and KGF by hCE into medium after 24-hour culture. The vertical axis represents logarithmic scale. N.D., not detected.
Techniques Used:
30) Product Images from "(Pro)renin Receptor Triggers Distinct Angiotensin II-Independent Extracellular Matrix Remodeling and Deterioration of Cardiac Function"
Article Title: (Pro)renin Receptor Triggers Distinct Angiotensin II-Independent Extracellular Matrix Remodeling and Deterioration of Cardiac Function
Journal: PLoS ONE
doi: 10.1371/journal.pone.0041404

Figure Legend Snippet: Effect of intramyocardial (P)RR gene delivery on cardiac gene expression without or with the losartan (Los) treatment. A, atrial natriuretic peptide (ANP) mRNA, B, β-myosin heavy chain (β-MHC) mRNA, C, skeletal α-actin mRNA, D, α-myosin heavy chain (α-MHC), E, cardiac α-actin mRNA, F, sarcoplasmic reticulum Ca 2+ ATPase (SERCA2) mRNA, G, vascular endothelial growth factor (VEGF) mRNA at 2 weeks and H, fibroblast growth factor-2 (FGF-2) mRNA levels at 1 week. The results are expressed as mean±SEM (n = 8 to 10). * P
Techniques Used: Expressing, Aqueous Normal-phase Chromatography
31) Product Images from "EFFECT OF RHO-KINASE INHIBITOR, Y27632, ON PORCINE CORNEAL ENDOTHELIAL CELL CULTURE, INFLAMMATION AND IMMUNE REGULATION"
Article Title: EFFECT OF RHO-KINASE INHIBITOR, Y27632, ON PORCINE CORNEAL ENDOTHELIAL CELL CULTURE, INFLAMMATION AND IMMUNE REGULATION
Journal: Ocular immunology and inflammation
doi: 10.3109/09273948.2015.1056534

Figure Legend Snippet: Suppression of inflammatory chemokines from pCECs (A) MCP-1 and VEGF mRNA levels in pCECs with/without 10μM Y27632 were measured by real-time PCR after hTNF-α activation. Although there were increases in MCP-1 and VEGF mRNA in pCECs after activation, Y27632 significantly reduced MCP-1 and VEGF mRNA expression after 6h of activation (43% and 37% reduction respectively, *p
Techniques Used: Real-time Polymerase Chain Reaction, Activation Assay, Expressing
32) Product Images from "Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation"
Article Title: Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation
Journal: PLoS ONE
doi: 10.1371/journal.pone.0127003

Figure Legend Snippet: Immunological confirmation of findings. 2D-DIGE gel spot abundances for the FABP5 of the 21 induced sputum samples (A). Western blot analysis for 138 induced sputum samples (B) and for 158 nasal lavage fluid samples (C) with FABP5 antibody. ELISA results for vascular endothelial growth factor (VEGF) (D) and cysteinyl leukotriene (CysLT) (E) measurements from 90 nasal lavage fluid samples. The figures show mean with standard error of the mean. * p
Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay
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