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Santa Cruz Biotechnology vascular endothelial growth factor vegf
Mechanical ventilation increased MMP-7, cyclin D1, and <t>VEGF</t> in the lung. Densitometry analysis of the active form (20 kDa) of MMP-7 was normalized to the inactive form (30 kDa) and then normalized to <t>β-actin.</t> Densitometry analysis (in graph) and representative gels correspond to three different experimental protocols: spontaneous breathing animals (controls, C), ventilated with low V T and ventilated with high V T . (*) p
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1) Product Images from "Activation of the Wnt/?-Catenin Signaling Pathway by Mechanical Ventilation Is Associated with Ventilator-Induced Pulmonary Fibrosis in Healthy Lungs"

Article Title: Activation of the Wnt/?-Catenin Signaling Pathway by Mechanical Ventilation Is Associated with Ventilator-Induced Pulmonary Fibrosis in Healthy Lungs

Journal: PLoS ONE

doi: 10.1371/journal.pone.0023914

Mechanical ventilation increased MMP-7, cyclin D1, and VEGF in the lung. Densitometry analysis of the active form (20 kDa) of MMP-7 was normalized to the inactive form (30 kDa) and then normalized to β-actin. Densitometry analysis (in graph) and representative gels correspond to three different experimental protocols: spontaneous breathing animals (controls, C), ventilated with low V T and ventilated with high V T . (*) p
Figure Legend Snippet: Mechanical ventilation increased MMP-7, cyclin D1, and VEGF in the lung. Densitometry analysis of the active form (20 kDa) of MMP-7 was normalized to the inactive form (30 kDa) and then normalized to β-actin. Densitometry analysis (in graph) and representative gels correspond to three different experimental protocols: spontaneous breathing animals (controls, C), ventilated with low V T and ventilated with high V T . (*) p

Techniques Used:

2) Product Images from "miR-566 expression and immune changes in patients with intracranial aneurysm"

Article Title: miR-566 expression and immune changes in patients with intracranial aneurysm

Journal: International Journal of Clinical and Experimental Pathology

doi:

Linear analysis of miR-566 expression level with VHL (A: r =0.81, P =6.7 e9 ), VEGF (B: r =0.77, P =8.3 -e8 ), NIK (C: r =0.89, P =9.3 e8 ) and ALOX5 (D: r =0.63, P =8.2 -e7 ) gene expression.
Figure Legend Snippet: Linear analysis of miR-566 expression level with VHL (A: r =0.81, P =6.7 e9 ), VEGF (B: r =0.77, P =8.3 -e8 ), NIK (C: r =0.89, P =9.3 e8 ) and ALOX5 (D: r =0.63, P =8.2 -e7 ) gene expression.

Techniques Used: Expressing

Protein expression of immune-related controlling genes detected byt western blotting. A: VHL, NIK, VEGF and ALOX5 expression in IA detected by western blotting, B: Statistical results of VHL, NIK, VEGF and ALOX5 expression in IA (VHL: ** P =0.0073, NIK: * P =0.0030, VEGF: ** P =0.0053, ALOX5: ** P =0.00449).
Figure Legend Snippet: Protein expression of immune-related controlling genes detected byt western blotting. A: VHL, NIK, VEGF and ALOX5 expression in IA detected by western blotting, B: Statistical results of VHL, NIK, VEGF and ALOX5 expression in IA (VHL: ** P =0.0073, NIK: * P =0.0030, VEGF: ** P =0.0053, ALOX5: ** P =0.00449).

Techniques Used: Expressing, Western Blot

mRNA relative expression of immune-related controlling genes detected by qPCR: VEGF (A, ** P =0.0085), ALOX5 (B, *** P =0.00075), VHL (C, *** P =0.0091) and NIK (D, ** P =0.0097).
Figure Legend Snippet: mRNA relative expression of immune-related controlling genes detected by qPCR: VEGF (A, ** P =0.0085), ALOX5 (B, *** P =0.00075), VHL (C, *** P =0.0091) and NIK (D, ** P =0.0097).

Techniques Used: Expressing, Real-time Polymerase Chain Reaction

3) Product Images from "Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Precursor Cells"

Article Title: Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Precursor Cells

Journal: Rejuvenation Research

doi: 10.1089/rej.2013.1468

Buyang Huanwu Decoction (BYHWD) increases expression of vascular endothelial growth factor (VEGF), Reelin, and brain-derived neurotrophic factor (BDNF) in the ischemic hemisphere after middle cerebral artery occlusion (MCAO). Representative microphotographs
Figure Legend Snippet: Buyang Huanwu Decoction (BYHWD) increases expression of vascular endothelial growth factor (VEGF), Reelin, and brain-derived neurotrophic factor (BDNF) in the ischemic hemisphere after middle cerebral artery occlusion (MCAO). Representative microphotographs

Techniques Used: Expressing, Derivative Assay

4) Product Images from "Cripto Enhances Proliferation and Survival of Mesenchymal Stem Cells by Up-Regulating JAK2/STAT3 Pathway in a GRP78-Dependent Manner"

Article Title: Cripto Enhances Proliferation and Survival of Mesenchymal Stem Cells by Up-Regulating JAK2/STAT3 Pathway in a GRP78-Dependent Manner

Journal: Biomolecules & Therapeutics

doi: 10.4062/biomolther.2017.099

Schematic representation of the putative mechanisms by which Cripto increases proliferation, survival, and enhances secretion of growth factors in MSCs. Exposure to Cripto increases the proliferative potential of MSCs via the activation of the JAK2/STAT3 pathway and production of growth factors, such as FGF, VEGF, and HGF. In addition, Cripto promotes MSC survival by augmenting BCL3 expression in a STAT3-dependent manner.
Figure Legend Snippet: Schematic representation of the putative mechanisms by which Cripto increases proliferation, survival, and enhances secretion of growth factors in MSCs. Exposure to Cripto increases the proliferative potential of MSCs via the activation of the JAK2/STAT3 pathway and production of growth factors, such as FGF, VEGF, and HGF. In addition, Cripto promotes MSC survival by augmenting BCL3 expression in a STAT3-dependent manner.

Techniques Used: Activation Assay, Expressing

Effect of Cripto on the expression of vascularization factors. MSCs were pre-transfected with STAT3 siRNA or non-targeting scrambled siRNA for 24 h before the treatment with 100 ng/mL Cripto for 24 h. (A) Total protein was extracted and immunoblotted with an anti-VEGF antibody. Amounts of β-actin were used as internal loading controls. Lower panel indicates mean normalized levels (± SEM) of VEGF (n=3). (B) Measurements of human VEGF concentration in MSCs using ELISA (n=3). (C) Total protein was extracted and immunoblotted with an anti-FGF antibody (n=3). Amounts of β-actin were used as internal loading controls. Lower panel indicates mean normalized levels (± SEM) of FGF. (D) Measurements of human FGF concentration in MSCs using ELISA (n=3). (E) Total protein was extracted and immunoblotted with an anti-HGF antibody. Amounts of β-actin were used as internal loading controls. Lower panel indicates mean normalized levels (± SEM) of HGF (n=3). (F) Measurements of human HGF concentration in MSCs using ELISA (n=3). Statistical significance is indicated as follows: ** p
Figure Legend Snippet: Effect of Cripto on the expression of vascularization factors. MSCs were pre-transfected with STAT3 siRNA or non-targeting scrambled siRNA for 24 h before the treatment with 100 ng/mL Cripto for 24 h. (A) Total protein was extracted and immunoblotted with an anti-VEGF antibody. Amounts of β-actin were used as internal loading controls. Lower panel indicates mean normalized levels (± SEM) of VEGF (n=3). (B) Measurements of human VEGF concentration in MSCs using ELISA (n=3). (C) Total protein was extracted and immunoblotted with an anti-FGF antibody (n=3). Amounts of β-actin were used as internal loading controls. Lower panel indicates mean normalized levels (± SEM) of FGF. (D) Measurements of human FGF concentration in MSCs using ELISA (n=3). (E) Total protein was extracted and immunoblotted with an anti-HGF antibody. Amounts of β-actin were used as internal loading controls. Lower panel indicates mean normalized levels (± SEM) of HGF (n=3). (F) Measurements of human HGF concentration in MSCs using ELISA (n=3). Statistical significance is indicated as follows: ** p

Techniques Used: Expressing, Transfection, Concentration Assay, Enzyme-linked Immunosorbent Assay

5) Product Images from "Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema"

Article Title: Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema

Journal: Stem Cells International

doi: 10.1155/2018/9492038

Growth factor profile and MSCs. (a–c) Protein expression of epidermal growth factor (EGF) (a), vascular endothelial growth factor (VEGF) (b), and hepatocyte growth factor (HGF) (c) in the lung by Western blotting. (d) Negative control for HGF staining. (e) Representative images displaying HGF (red) in the proximity of GFP-positive MSCs (green). (f) Intracellular content of HGF (red) in a GFP-positive MSC (green). (g–i) c-Met expression (red) in the lung parenchyma from the naïve (g), PPE (h), and PPE-MSC (i) groups; alveolar type II epithelial cells are shown by SFTPC (green). Nuclei are counterstained with DAPI (blue). Data are expressed as the mean ± SD ( n = 6 in each experimental group). Scale bars: 10 μ m (f) and 20 μ m (g–i). ∗ P
Figure Legend Snippet: Growth factor profile and MSCs. (a–c) Protein expression of epidermal growth factor (EGF) (a), vascular endothelial growth factor (VEGF) (b), and hepatocyte growth factor (HGF) (c) in the lung by Western blotting. (d) Negative control for HGF staining. (e) Representative images displaying HGF (red) in the proximity of GFP-positive MSCs (green). (f) Intracellular content of HGF (red) in a GFP-positive MSC (green). (g–i) c-Met expression (red) in the lung parenchyma from the naïve (g), PPE (h), and PPE-MSC (i) groups; alveolar type II epithelial cells are shown by SFTPC (green). Nuclei are counterstained with DAPI (blue). Data are expressed as the mean ± SD ( n = 6 in each experimental group). Scale bars: 10 μ m (f) and 20 μ m (g–i). ∗ P

Techniques Used: Expressing, Western Blot, Negative Control, Staining

6) Product Images from "Stress-Induced Activation of Apoptosis Signal-Regulating Kinase 1 Promotes Osteoarthritis"

Article Title: Stress-Induced Activation of Apoptosis Signal-Regulating Kinase 1 Promotes Osteoarthritis

Journal: Journal of cellular physiology

doi: 10.1002/jcp.25186

Knocking out ASK1 decreases chondrocyte maturation and MAPK signaling in response to stress. (A) Fluorescence immunohistochemistry of the growth plate for ASK1 shows increased expression with terminal differentiation and the highest intensity in hypertrophic chondrocytes where cells also stain positive with TUNEL. (B) Immunohistochemistry performed on postnatal D14 mouse growth plates shows an increase in VEGF, MMP13, and COL-X in the WT during chondrocyte maturation, with decreased staining in the KO animals. Examination of all growth plates showed an increase over WT in the length of the hypertrophic zone (HZ-brackets) in the KO mice. (C) WT MEFs grown in chondrogenic media (13 days) treated with TNFα or IL-1β (for 24 h) showed increased phosphorylation of JNK and p38, as well as increased expression of NFκB, all of which were decreased in the KO cells. Graphs show densitometric analysis of the Westerns (percentage relative to untreated WT). (D) Similar increases in markers of chondrocyte maturation, COL-X, MMP13, and VEGF were observed in WT MEFs after treatment, but not in KO MEFs and graph indicates quantification. (n = 4; * = P ≤ 0.05; ** = P ≤ 0.01).
Figure Legend Snippet: Knocking out ASK1 decreases chondrocyte maturation and MAPK signaling in response to stress. (A) Fluorescence immunohistochemistry of the growth plate for ASK1 shows increased expression with terminal differentiation and the highest intensity in hypertrophic chondrocytes where cells also stain positive with TUNEL. (B) Immunohistochemistry performed on postnatal D14 mouse growth plates shows an increase in VEGF, MMP13, and COL-X in the WT during chondrocyte maturation, with decreased staining in the KO animals. Examination of all growth plates showed an increase over WT in the length of the hypertrophic zone (HZ-brackets) in the KO mice. (C) WT MEFs grown in chondrogenic media (13 days) treated with TNFα or IL-1β (for 24 h) showed increased phosphorylation of JNK and p38, as well as increased expression of NFκB, all of which were decreased in the KO cells. Graphs show densitometric analysis of the Westerns (percentage relative to untreated WT). (D) Similar increases in markers of chondrocyte maturation, COL-X, MMP13, and VEGF were observed in WT MEFs after treatment, but not in KO MEFs and graph indicates quantification. (n = 4; * = P ≤ 0.05; ** = P ≤ 0.01).

Techniques Used: Fluorescence, Immunohistochemistry, Expressing, Staining, TUNEL Assay, Mouse Assay

ASK1 Promotes Chondrocyte Maturation and Hypertrophy in Articular Cartilage with OA. (A) Immunohistochemistry performed on injury-induced OA mouse limbs clearly shows the presence of ASK1 in WT articular cartilage, but not in the KO mouse cartilage. Accompanying the presence in ASK1 in WT articular chondrocytes was an increase in (B) H2AX, (C) cl-PARP (D) VEGF, (E) COL-X, and (F) MMP13, all of which were decreased in KO animals. (n = 2 mice-3 images each; scale bar = 100μm).
Figure Legend Snippet: ASK1 Promotes Chondrocyte Maturation and Hypertrophy in Articular Cartilage with OA. (A) Immunohistochemistry performed on injury-induced OA mouse limbs clearly shows the presence of ASK1 in WT articular cartilage, but not in the KO mouse cartilage. Accompanying the presence in ASK1 in WT articular chondrocytes was an increase in (B) H2AX, (C) cl-PARP (D) VEGF, (E) COL-X, and (F) MMP13, all of which were decreased in KO animals. (n = 2 mice-3 images each; scale bar = 100μm).

Techniques Used: Immunohistochemistry, Mouse Assay

7) Product Images from "Myeloid cell leukemia-1 is associated with tumor progression by inhibiting apoptosis and enhancing angiogenesis in colorectal cancer"

Article Title: Myeloid cell leukemia-1 is associated with tumor progression by inhibiting apoptosis and enhancing angiogenesis in colorectal cancer

Journal: American Journal of Cancer Research

doi:

The impact of Mcl-1 knockdown on angiogenesis of human colorectal cancer cells. A. The Invasion of HUVECs was significantly decreased in the conditioned medium (CM) from Mcl-1 siRNA-transfected Caco2 and DLD1 cells compared to the scramble siRNA-transfected cells. B. CM from Mcl-1 siRNA-transfected Caco2 and DLD1 cells inhibited the tube formation of HUVECs compared to CM from the scramble siRNA-transfected cells. C. Mcl-1 knockdown led to a decrease of the angiogenic inducer, VEGF, in Caco2 and DLD1 cells. However, the another angiogenic inducer, HIF-1α was not altered in response to Mcl-1 knockdown in all tested cells. SS; scramble siRNA, MS; Mcl-1 siRNA, VEGF; vascular endothelial growth factor, HIF-1α; Hypoxia inducible factor-1α; *p
Figure Legend Snippet: The impact of Mcl-1 knockdown on angiogenesis of human colorectal cancer cells. A. The Invasion of HUVECs was significantly decreased in the conditioned medium (CM) from Mcl-1 siRNA-transfected Caco2 and DLD1 cells compared to the scramble siRNA-transfected cells. B. CM from Mcl-1 siRNA-transfected Caco2 and DLD1 cells inhibited the tube formation of HUVECs compared to CM from the scramble siRNA-transfected cells. C. Mcl-1 knockdown led to a decrease of the angiogenic inducer, VEGF, in Caco2 and DLD1 cells. However, the another angiogenic inducer, HIF-1α was not altered in response to Mcl-1 knockdown in all tested cells. SS; scramble siRNA, MS; Mcl-1 siRNA, VEGF; vascular endothelial growth factor, HIF-1α; Hypoxia inducible factor-1α; *p

Techniques Used: Transfection, Mass Spectrometry

8) Product Images from "Exosomes Derived from Akt‐Modified Human Umbilical Cord Mesenchymal Stem Cells Improve Cardiac Regeneration and Promote Angiogenesis via Activating Platelet‐Derived Growth Factor D"

Article Title: Exosomes Derived from Akt‐Modified Human Umbilical Cord Mesenchymal Stem Cells Improve Cardiac Regeneration and Promote Angiogenesis via Activating Platelet‐Derived Growth Factor D

Journal: Stem Cells Translational Medicine

doi: 10.5966/sctm.2016-0038

PDGF‐D/PDGFR is involved in Akt‐Exo‐mediated improvement of myocardial repair. (A): Representative images of Western blot on the expression of VEGF, CD31, PDGF‐D, and TGF‐β in exosomes. (B, C): Western blot of the expression of PDGF‐D (B) and semiquantitative ratio of PDGF‐D to CD63 (C) . (D, E): Representative images of Western blot of the expression of PDGFR‐β in myocardium (D) and semiquantitative data of PDGFR‐β to GAPDH (E) . ∗∗∗, p
Figure Legend Snippet: PDGF‐D/PDGFR is involved in Akt‐Exo‐mediated improvement of myocardial repair. (A): Representative images of Western blot on the expression of VEGF, CD31, PDGF‐D, and TGF‐β in exosomes. (B, C): Western blot of the expression of PDGF‐D (B) and semiquantitative ratio of PDGF‐D to CD63 (C) . (D, E): Representative images of Western blot of the expression of PDGFR‐β in myocardium (D) and semiquantitative data of PDGFR‐β to GAPDH (E) . ∗∗∗, p

Techniques Used: Western Blot, Expressing

9) Product Images from "Nobiletin alleviates endometriosis via down-regulating NF-κB activity in endometriosis mouse model"

Article Title: Nobiletin alleviates endometriosis via down-regulating NF-κB activity in endometriosis mouse model

Journal: Bioscience Reports

doi: 10.1042/BSR20180470

Effects of nobiletin on the expression of proliferation and angiogenesis relevant genes in ectopic endometrium in vivo ( A ) Immunohistochemistry analysis of PCNA, VEGF, and E-cadherin. ( B ) The staining levels of PCNA, VEGF, and E-cadherin in different treatment groups. Abbreviations used in the figure: EM, endometriosis mice without treatment; N10, endometriosis mice treated 10 mg/kg/day; N20, endometriosis mice treated 20 mg/kg/day; N =6 mice per group. Values are expressed as means ± S.E.M. of three independent experiments; ** P
Figure Legend Snippet: Effects of nobiletin on the expression of proliferation and angiogenesis relevant genes in ectopic endometrium in vivo ( A ) Immunohistochemistry analysis of PCNA, VEGF, and E-cadherin. ( B ) The staining levels of PCNA, VEGF, and E-cadherin in different treatment groups. Abbreviations used in the figure: EM, endometriosis mice without treatment; N10, endometriosis mice treated 10 mg/kg/day; N20, endometriosis mice treated 20 mg/kg/day; N =6 mice per group. Values are expressed as means ± S.E.M. of three independent experiments; ** P

Techniques Used: Expressing, In Vivo, Immunohistochemistry, Staining, Mouse Assay

10) Product Images from "Systems pharmacology-based approach for dissecting the active ingredients and potential targets of the Chinese herbal Bufei Jianpi formula for the treatment of COPD"

Article Title: Systems pharmacology-based approach for dissecting the active ingredients and potential targets of the Chinese herbal Bufei Jianpi formula for the treatment of COPD

Journal: International Journal of Chronic Obstructive Pulmonary Disease

doi: 10.2147/COPD.S94043

Effect of BJF and APL on the expression of VEGF, bFGF, TGF-β, and ET-1 around the right ventricle obtained from chronic obstructive pulmonary disease rats. Notes: Immunohistochemical staining of the right ventricle sections for VEGF, bFGF, TGF-β, and ET-1 (original magnification ×200) ( A ). Quantitative analysis for the expression of VEGF, bFGF, TGF-β, and ET-1 in the right ventricle ( B ). Values represent the mean ± standard error of mean. ** P
Figure Legend Snippet: Effect of BJF and APL on the expression of VEGF, bFGF, TGF-β, and ET-1 around the right ventricle obtained from chronic obstructive pulmonary disease rats. Notes: Immunohistochemical staining of the right ventricle sections for VEGF, bFGF, TGF-β, and ET-1 (original magnification ×200) ( A ). Quantitative analysis for the expression of VEGF, bFGF, TGF-β, and ET-1 in the right ventricle ( B ). Values represent the mean ± standard error of mean. ** P

Techniques Used: Expressing, Immunohistochemistry, Staining

11) Product Images from "Transplantation of bradykinin-preconditioned human endothelial progenitor cells improves cardiac function via enhanced Akt/eNOS phosphorylation and angiogenesis"

Article Title: Transplantation of bradykinin-preconditioned human endothelial progenitor cells improves cardiac function via enhanced Akt/eNOS phosphorylation and angiogenesis

Journal: American Journal of Translational Research

doi:

Effects of BK-PC-hEPC transplantation on Akt, eNOS, VEGF expression, as well as NO production. A and B. Western blots analysis to identify Akt and eNOS expressions. C. NO production in myocardial tissue. D. Western blot analysis for VEGF expression. Representative
Figure Legend Snippet: Effects of BK-PC-hEPC transplantation on Akt, eNOS, VEGF expression, as well as NO production. A and B. Western blots analysis to identify Akt and eNOS expressions. C. NO production in myocardial tissue. D. Western blot analysis for VEGF expression. Representative

Techniques Used: Transplantation Assay, Expressing, Western Blot

12) Product Images from "Differential stress response mechanisms in right and left ventricles"

Article Title: Differential stress response mechanisms in right and left ventricles

Journal: Journal of rare diseases research & treatment

doi:

Differential expression of angiogenesis markers in the RV and LV free walls RV and LV free walls were isolated from male SD rats and homogenized. Homogenates were subjected to Western blotting to monitor the expression of (a) PECAM-1 and (b) VEGF proteins. Bar graphs represent means ± SEM of the ratio of PECAM-1 or VEGF to G3PDH. The symbol * denotes that RV and LV values are significantly different from each other at P
Figure Legend Snippet: Differential expression of angiogenesis markers in the RV and LV free walls RV and LV free walls were isolated from male SD rats and homogenized. Homogenates were subjected to Western blotting to monitor the expression of (a) PECAM-1 and (b) VEGF proteins. Bar graphs represent means ± SEM of the ratio of PECAM-1 or VEGF to G3PDH. The symbol * denotes that RV and LV values are significantly different from each other at P

Techniques Used: Expressing, Isolation, Western Blot

13) Product Images from "Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema"

Article Title: Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema

Journal: Stem Cells International

doi: 10.1155/2018/9492038

Growth factor profile and MSCs. (a–c) Protein expression of epidermal growth factor (EGF) (a), vascular endothelial growth factor (VEGF) (b), and hepatocyte growth factor (HGF) (c) in the lung by Western blotting. (d) Negative control for HGF staining. (e) Representative images displaying HGF (red) in the proximity of GFP-positive MSCs (green). (f) Intracellular content of HGF (red) in a GFP-positive MSC (green). (g–i) c-Met expression (red) in the lung parenchyma from the naïve (g), PPE (h), and PPE-MSC (i) groups; alveolar type II epithelial cells are shown by SFTPC (green). Nuclei are counterstained with DAPI (blue). Data are expressed as the mean ± SD ( n = 6 in each experimental group). Scale bars: 10 μ m (f) and 20 μ m (g–i). ∗ P
Figure Legend Snippet: Growth factor profile and MSCs. (a–c) Protein expression of epidermal growth factor (EGF) (a), vascular endothelial growth factor (VEGF) (b), and hepatocyte growth factor (HGF) (c) in the lung by Western blotting. (d) Negative control for HGF staining. (e) Representative images displaying HGF (red) in the proximity of GFP-positive MSCs (green). (f) Intracellular content of HGF (red) in a GFP-positive MSC (green). (g–i) c-Met expression (red) in the lung parenchyma from the naïve (g), PPE (h), and PPE-MSC (i) groups; alveolar type II epithelial cells are shown by SFTPC (green). Nuclei are counterstained with DAPI (blue). Data are expressed as the mean ± SD ( n = 6 in each experimental group). Scale bars: 10 μ m (f) and 20 μ m (g–i). ∗ P

Techniques Used: Expressing, Western Blot, Negative Control, Staining

14) Product Images from "Ablation of SIRT3 Causes Coronary Microvascular Dysfunction and Impairs Cardiac Recovery Post Myocardial Ischemia"

Article Title: Ablation of SIRT3 Causes Coronary Microvascular Dysfunction and Impairs Cardiac Recovery Post Myocardial Ischemia

Journal: International journal of cardiology

doi: 10.1016/j.ijcard.2016.04.092

SIRT3 deletion reduces expression of angiogenic growth factors and PFKFB3 post MI. A–D. The expressions of Ang-1, VEGF, VEGFR2 and PFKFB3 were significantly lower in the heart of SIRT3 KO mice (n=5) than that of WT mice (n=6). n=5–6 per
Figure Legend Snippet: SIRT3 deletion reduces expression of angiogenic growth factors and PFKFB3 post MI. A–D. The expressions of Ang-1, VEGF, VEGFR2 and PFKFB3 were significantly lower in the heart of SIRT3 KO mice (n=5) than that of WT mice (n=6). n=5–6 per

Techniques Used: Expressing, Mouse Assay

15) Product Images from "VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase"

Article Title: VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase

Journal: ISRN Dermatology

doi: 10.5402/2012/941465

Effect of tryptase on the VEGF, Flt-1, and Flk-1 mRNA levels in HDMECs. Different concentrations of tryptase (0, 1, and 10 nmol/L) were added into HDMECs for 6 h. The mRNA levels of VEGF (a), Flt-1 (b), and Flk-1 (c) were determined by Real-time RT-PCR and normalized to GAPDH. The heparin control was also analyzed. * P
Figure Legend Snippet: Effect of tryptase on the VEGF, Flt-1, and Flk-1 mRNA levels in HDMECs. Different concentrations of tryptase (0, 1, and 10 nmol/L) were added into HDMECs for 6 h. The mRNA levels of VEGF (a), Flt-1 (b), and Flk-1 (c) were determined by Real-time RT-PCR and normalized to GAPDH. The heparin control was also analyzed. * P

Techniques Used: Quantitative RT-PCR

Effect of tryptase on the VEGF, Flt-1, and Flk-1 protein levels in HDMECs with or without APC366. HDMECs were treated with different concentrations of tryptase for 18 h in the absence or presence of APC366 (250 μ g/mL). The protein levels of VEGF (a), Flt-1 (b), and Flk-1 (c) were determined by Western blot and normalized to GAPDH. The heparin control was also analyzed. * P
Figure Legend Snippet: Effect of tryptase on the VEGF, Flt-1, and Flk-1 protein levels in HDMECs with or without APC366. HDMECs were treated with different concentrations of tryptase for 18 h in the absence or presence of APC366 (250 μ g/mL). The protein levels of VEGF (a), Flt-1 (b), and Flk-1 (c) were determined by Western blot and normalized to GAPDH. The heparin control was also analyzed. * P

Techniques Used: Western Blot

16) Product Images from "Rosiglitazone Inhibits Bone Regeneration and Causes Significant Accumulation of Fat at Sites of New Bone Formation"

Article Title: Rosiglitazone Inhibits Bone Regeneration and Causes Significant Accumulation of Fat at Sites of New Bone Formation

Journal: Calcified tissue international

doi: 10.1007/s00223-012-9623-4

Immunohistochemical analyses of PCNA-positive and hematopoietic VEGF-producing cells and the number of hematopoietic sinusoids at the DO gap after 2 weeks of distraction. a Visualization and quantification of cells stained for PCNA ( brown ) on the surface
Figure Legend Snippet: Immunohistochemical analyses of PCNA-positive and hematopoietic VEGF-producing cells and the number of hematopoietic sinusoids at the DO gap after 2 weeks of distraction. a Visualization and quantification of cells stained for PCNA ( brown ) on the surface

Techniques Used: Immunohistochemistry, Staining

17) Product Images from "Systems pharmacology-based dissection of mechanisms of Chinese medicinal formula Bufei Yishen as an effective treatment for chronic obstructive pulmonary disease"

Article Title: Systems pharmacology-based dissection of mechanisms of Chinese medicinal formula Bufei Yishen as an effective treatment for chronic obstructive pulmonary disease

Journal: Scientific Reports

doi: 10.1038/srep15290

Effect of Bufei Yishen formula (BYF) on the expression of VEGF, bFGF, TGF-β, and ET-1 in the right ventricle obtained from chronic obstructive pulmonary disease (COPD) rats. COPD rats were intragastricly treated with BYF (4.44 g/kg) and aminophylline (APL, 2.3 mg/kg) once daily. VEGF, bFGF, TGF-β and ET-1 in right ventricle of COPD rats expression were analyzed by immunohistochemistry (magnification, ×100) ( A ). The expression of VEGF, bFGF, TGF-β and ET-1 was quantitatively analyzed ( B ). Values represent means ± SEM, n = 8. ** P
Figure Legend Snippet: Effect of Bufei Yishen formula (BYF) on the expression of VEGF, bFGF, TGF-β, and ET-1 in the right ventricle obtained from chronic obstructive pulmonary disease (COPD) rats. COPD rats were intragastricly treated with BYF (4.44 g/kg) and aminophylline (APL, 2.3 mg/kg) once daily. VEGF, bFGF, TGF-β and ET-1 in right ventricle of COPD rats expression were analyzed by immunohistochemistry (magnification, ×100) ( A ). The expression of VEGF, bFGF, TGF-β and ET-1 was quantitatively analyzed ( B ). Values represent means ± SEM, n = 8. ** P

Techniques Used: Expressing, Immunohistochemistry

18) Product Images from "Ablation of endothelial prolyl hydroxylase domain protein‐2 promotes renal vascular remodelling and fibrosis in mice"

Article Title: Ablation of endothelial prolyl hydroxylase domain protein‐2 promotes renal vascular remodelling and fibrosis in mice

Journal: Journal of Cellular and Molecular Medicine

doi: 10.1111/jcmm.13117

The expression of pericyte‐associated factors in the kidney. ( A‐G ) Western blot showed that the expression of HIF‐1α, HIF‐2α, Ang‐1, Ang‐2, VEGF, VEGFR2 and NOTCH3 was significantly increased in the renal cortex of PHD2 EC KO mice compared to those of the control PHD2 f/f mice ( n = 6–7 mice). ( H ) The immunofluorescence study further confirmed that NOTCH3 (red) was up‐regulated in the kidney of PHD2 EC KO mice compared to the control mice especially around the vessel and in the glomerulus (white arrows) ( n = 9 mice/group). Mean ± S.E.M., * P
Figure Legend Snippet: The expression of pericyte‐associated factors in the kidney. ( A‐G ) Western blot showed that the expression of HIF‐1α, HIF‐2α, Ang‐1, Ang‐2, VEGF, VEGFR2 and NOTCH3 was significantly increased in the renal cortex of PHD2 EC KO mice compared to those of the control PHD2 f/f mice ( n = 6–7 mice). ( H ) The immunofluorescence study further confirmed that NOTCH3 (red) was up‐regulated in the kidney of PHD2 EC KO mice compared to the control mice especially around the vessel and in the glomerulus (white arrows) ( n = 9 mice/group). Mean ± S.E.M., * P

Techniques Used: Expressing, Western Blot, Mouse Assay, Immunofluorescence

19) Product Images from "A novel microtubule-modulating agent EM011 inhibits angiogenesis by repressing the HIF-1? axis and disrupting cell polarity and migration"

Article Title: A novel microtubule-modulating agent EM011 inhibits angiogenesis by repressing the HIF-1? axis and disrupting cell polarity and migration

Journal: Carcinogenesis

doi: 10.1093/carcin/bgs200

EM011 induces transcriptional repression of HIF-1α and its downstream target genes, VEGF and survivin. ( Ai) EM011 decreases mRNA levels of HIF-1α, survivin and VEGF. PC-3 cells were treated with 25 µM drug in the presence or absence
Figure Legend Snippet: EM011 induces transcriptional repression of HIF-1α and its downstream target genes, VEGF and survivin. ( Ai) EM011 decreases mRNA levels of HIF-1α, survivin and VEGF. PC-3 cells were treated with 25 µM drug in the presence or absence

Techniques Used:

20) Product Images from "Effects of resistin-like molecule ? over-expression on gastric cancer cells in vitro"

Article Title: Effects of resistin-like molecule ? over-expression on gastric cancer cells in vitro

Journal: World Journal of Gastroenterology : WJG

doi: 10.3748/wjg.v18.i8.754

Over-expression of resistin-like molecule β decreased the expression of vascular endothelial growth factor, but not v-ets erythroblastosis virus E26 oncogene homolog 1, in gastric cancer cells. A: Western blotting indicated that 72 h after transfection,
Figure Legend Snippet: Over-expression of resistin-like molecule β decreased the expression of vascular endothelial growth factor, but not v-ets erythroblastosis virus E26 oncogene homolog 1, in gastric cancer cells. A: Western blotting indicated that 72 h after transfection,

Techniques Used: Over Expression, Expressing, Western Blot, Transfection

21) Product Images from "The absence of the CD163 receptor has distinct temporal influences on intracerebral hemorrhage outcomes"

Article Title: The absence of the CD163 receptor has distinct temporal influences on intracerebral hemorrhage outcomes

Journal: Journal of Cerebral Blood Flow & Metabolism

doi: 10.1177/0271678X17701459

Effect of CD163 deficiency on angiogenesis/neovascularization. Representative images showing ipsilateral cortical and hematomal immunoreactivity of (a) PECAM at 3 d, (d) VEGF at 3 d, and (g) VEGF at 10 d for WT and CD163 −/− mice. Square selections on the low magnification center hemi-brain images denote the location of magnified regions. (b) At 3 d, CD163 −/− mice have significantly increased neovascularization in the motor cortex. (c,e,f) No difference in hematomal neovascularization or cortical and hematomal VEGF expression is seen. (h–i) At 10 d, CD163 −/− mice have increased hematomal VEGF expression, but no difference in cortical expression. At 3 d, comparisons include n = 5 WT and n = 7 CD163 −/− mice for PECAM and n = 7 WT and n = 7 CD163 −/− mice for VEGF. At 10 d, comparisons include n = 6 WT and n = 10 CD163 −/− mice. *p
Figure Legend Snippet: Effect of CD163 deficiency on angiogenesis/neovascularization. Representative images showing ipsilateral cortical and hematomal immunoreactivity of (a) PECAM at 3 d, (d) VEGF at 3 d, and (g) VEGF at 10 d for WT and CD163 −/− mice. Square selections on the low magnification center hemi-brain images denote the location of magnified regions. (b) At 3 d, CD163 −/− mice have significantly increased neovascularization in the motor cortex. (c,e,f) No difference in hematomal neovascularization or cortical and hematomal VEGF expression is seen. (h–i) At 10 d, CD163 −/− mice have increased hematomal VEGF expression, but no difference in cortical expression. At 3 d, comparisons include n = 5 WT and n = 7 CD163 −/− mice for PECAM and n = 7 WT and n = 7 CD163 −/− mice for VEGF. At 10 d, comparisons include n = 6 WT and n = 10 CD163 −/− mice. *p

Techniques Used: Mouse Assay, Expressing

22) Product Images from "Anti-angiogenic effects of epigallocatechin-3-gallate in human skin"

Article Title: Anti-angiogenic effects of epigallocatechin-3-gallate in human skin

Journal: International Journal of Clinical and Experimental Pathology

doi:

Bar graph showing the mean values ± SEM of positively stained epidermis in skin sections obtained from 4 subjects and analyzed for HIF-1α and VEGF via immunohistochemistry. Six weeks of treatment with EGCG cream (closed bars) resulted
Figure Legend Snippet: Bar graph showing the mean values ± SEM of positively stained epidermis in skin sections obtained from 4 subjects and analyzed for HIF-1α and VEGF via immunohistochemistry. Six weeks of treatment with EGCG cream (closed bars) resulted

Techniques Used: Staining, Immunohistochemistry

Representative immunohistochemistry images of VEGF staining in vehicle (A) treated versus EGCG (B) treated skin. Tissue sections were stained with VEGF rabbit polyclonal antibody according to the protocol described in the methods section. Epidermal areas
Figure Legend Snippet: Representative immunohistochemistry images of VEGF staining in vehicle (A) treated versus EGCG (B) treated skin. Tissue sections were stained with VEGF rabbit polyclonal antibody according to the protocol described in the methods section. Epidermal areas

Techniques Used: Immunohistochemistry, Staining

23) Product Images from "Prevascularisation with endothelial progenitor cells improved restoration of the architectural and functional properties of newly formed bone for bone reconstruction"

Article Title: Prevascularisation with endothelial progenitor cells improved restoration of the architectural and functional properties of newly formed bone for bone reconstruction

Journal: International Orthopaedics

doi: 10.1007/s00264-012-1751-y

Morphological and histological observation. Axial view (4× magnification) of Chinese-ink microangiographs at 12 weeks post operation showed that the cross-sectional morphology of the medullary cavity and intraosseous vasculatures, including intramedullary blood vessels in group A ( b ) was restored close to normal ( a ). However, the medullary vessels and medullary cavity were partly restored in group B ( c ) and barely rebuilt in group C ( d ). Early vascularization was enhanced by EPC prevascularization with formation of neovasculature expressed higher VEGF ( e ) and FVIII ( h ) immunopositivity at two weeks. In group B, the number of neovasculature was less than group A, and the expression of VEGF ( f ) and FVIII ( i ) was moderate positive. Few blood vessels with weakly positive expression of VEGF ( g ) and FVIII ( j ) could be observed in group C; bar length = 50 μm
Figure Legend Snippet: Morphological and histological observation. Axial view (4× magnification) of Chinese-ink microangiographs at 12 weeks post operation showed that the cross-sectional morphology of the medullary cavity and intraosseous vasculatures, including intramedullary blood vessels in group A ( b ) was restored close to normal ( a ). However, the medullary vessels and medullary cavity were partly restored in group B ( c ) and barely rebuilt in group C ( d ). Early vascularization was enhanced by EPC prevascularization with formation of neovasculature expressed higher VEGF ( e ) and FVIII ( h ) immunopositivity at two weeks. In group B, the number of neovasculature was less than group A, and the expression of VEGF ( f ) and FVIII ( i ) was moderate positive. Few blood vessels with weakly positive expression of VEGF ( g ) and FVIII ( j ) could be observed in group C; bar length = 50 μm

Techniques Used: Expressing

24) Product Images from "A translational model of chronic kidney disease in swine"

Article Title: A translational model of chronic kidney disease in swine

Journal: American Journal of Physiology - Renal Physiology

doi: 10.1152/ajprenal.00063.2018

Experimental chronic kidney disease (CKD) induced significant renal microvascular rarefaction and remodeling in both kidneys. A and B : representative pictures of renal microvascular (MV) density (3-dimensional μCT reconstruction) and quantification (MV density of microvessels of diameter between 0 and 200, 200 and 300, and 300 and 500 μm. C : quantification of MV media to lumen ratio (both kidneys). D : representative protein expression (3 bands/group) of VEGF and Flk-1 receptor in normal pigs and pigs after 14 wk of CKD. * P
Figure Legend Snippet: Experimental chronic kidney disease (CKD) induced significant renal microvascular rarefaction and remodeling in both kidneys. A and B : representative pictures of renal microvascular (MV) density (3-dimensional μCT reconstruction) and quantification (MV density of microvessels of diameter between 0 and 200, 200 and 300, and 300 and 500 μm. C : quantification of MV media to lumen ratio (both kidneys). D : representative protein expression (3 bands/group) of VEGF and Flk-1 receptor in normal pigs and pigs after 14 wk of CKD. * P

Techniques Used: Expressing

25) Product Images from "Addition of endothelial progenitor cells to renal revascularization restores medullary tubular oxygen consumption in swine renal artery stenosis"

Article Title: Addition of endothelial progenitor cells to renal revascularization restores medullary tubular oxygen consumption in swine renal artery stenosis

Journal: American Journal of Physiology - Renal Physiology

doi: 10.1152/ajprenal.00563.2011

Representative (two bands per group) immunoblots demonstrating protein expression of VEGF, endothelial nitric oxide synthase (eNOS), and P47 in the cortex ( A ) and VEGF, eNOS, hypoxia-inducible factor-1α (HIF-1α), P47, and TNF-α
Figure Legend Snippet: Representative (two bands per group) immunoblots demonstrating protein expression of VEGF, endothelial nitric oxide synthase (eNOS), and P47 in the cortex ( A ) and VEGF, eNOS, hypoxia-inducible factor-1α (HIF-1α), P47, and TNF-α

Techniques Used: Western Blot, Expressing

26) Product Images from "Methyl jasmonate abolishes the migration, invasion and angiogenesis of gastric cancer cells through down-regulation of matrix metalloproteinase 14"

Article Title: Methyl jasmonate abolishes the migration, invasion and angiogenesis of gastric cancer cells through down-regulation of matrix metalloproteinase 14

Journal: BMC Cancer

doi: 10.1186/1471-2407-13-74

Sub-cytotoxic MJ suppressed the Sp1 expression and binding on MMP-14 promoter in gastric cancer cells. A and B , cancerous ( C ) and adjacent non-neoplastic (N) tissues from twenty gastric cancer patients were collected for the analysis of Sp1, MMP-14, and VEGF expression. Western blot and real-time quantitative RT-PCR indicated that the expression of Sp1, MMP-14, and VEGF was significantly higher in gastric cancer tissues than that of adjacent neoplastic tissues. C , Pearson’s coefficient correlation analysis demonstrated a positive correlation between Sp1 protein and MMP-14 transcript levels in gastric cancer tissues (r = 0.917, P
Figure Legend Snippet: Sub-cytotoxic MJ suppressed the Sp1 expression and binding on MMP-14 promoter in gastric cancer cells. A and B , cancerous ( C ) and adjacent non-neoplastic (N) tissues from twenty gastric cancer patients were collected for the analysis of Sp1, MMP-14, and VEGF expression. Western blot and real-time quantitative RT-PCR indicated that the expression of Sp1, MMP-14, and VEGF was significantly higher in gastric cancer tissues than that of adjacent neoplastic tissues. C , Pearson’s coefficient correlation analysis demonstrated a positive correlation between Sp1 protein and MMP-14 transcript levels in gastric cancer tissues (r = 0.917, P

Techniques Used: Expressing, Binding Assay, Western Blot, Quantitative RT-PCR

Sub-cytotoxic MJ suppressed the expression of VEGF in gastric cancer cells. Human gastric cancer cell lines SGC-7901 and MKN-45 were incubated with sub-cytotoxic concentrations of MJ as indicated. A and B , western blot and real-time quantitative RT-PCR indicated that administration of sub-cytotoxic (0.1 and 0.2 mM) MJ to SGC-7901 and MKN-45 cells for 24 hrs resulted in a decrease in the expression of VEGF, when compared to that of solvent-treated (mock) cells. C and D , western blot and real-time quantitative RT-PCR indicated that administration of 0.2 mM MJ to SGC-7901 and MKN-45 cells for 6, 12, and 24 hrs, resulted in the decrease of VEGF expression in a time-dependent manner, than that of mock cells. E and F , 72 hrs post-transfection of MMP-14 expression vector into SGC-7901 and MKN-45 cells, western blot and real-time quantitative RT-PCR indicated the over-expressed MMP-14 and VEGF than that of empty vector-transfected (mock) cells. G and H , 24 hrs post-transfection of si-MMP14 (100 nmol/L) into SGC-7901 and MKN-45 cells, western blot and real-time quantitative RT-PCR indicated the down-regulated MMP-14 and VEGF than those transfected with scramble siRNA (si-Scb, 100 nmol/L). The symbols (* and △) indicate a significant decrease and a significant increase from mock or si-Scb, respectively.
Figure Legend Snippet: Sub-cytotoxic MJ suppressed the expression of VEGF in gastric cancer cells. Human gastric cancer cell lines SGC-7901 and MKN-45 were incubated with sub-cytotoxic concentrations of MJ as indicated. A and B , western blot and real-time quantitative RT-PCR indicated that administration of sub-cytotoxic (0.1 and 0.2 mM) MJ to SGC-7901 and MKN-45 cells for 24 hrs resulted in a decrease in the expression of VEGF, when compared to that of solvent-treated (mock) cells. C and D , western blot and real-time quantitative RT-PCR indicated that administration of 0.2 mM MJ to SGC-7901 and MKN-45 cells for 6, 12, and 24 hrs, resulted in the decrease of VEGF expression in a time-dependent manner, than that of mock cells. E and F , 72 hrs post-transfection of MMP-14 expression vector into SGC-7901 and MKN-45 cells, western blot and real-time quantitative RT-PCR indicated the over-expressed MMP-14 and VEGF than that of empty vector-transfected (mock) cells. G and H , 24 hrs post-transfection of si-MMP14 (100 nmol/L) into SGC-7901 and MKN-45 cells, western blot and real-time quantitative RT-PCR indicated the down-regulated MMP-14 and VEGF than those transfected with scramble siRNA (si-Scb, 100 nmol/L). The symbols (* and △) indicate a significant decrease and a significant increase from mock or si-Scb, respectively.

Techniques Used: Expressing, Incubation, Western Blot, Quantitative RT-PCR, Transfection, Plasmid Preparation

Restoration of MMP-14 rescued sub-cytotoxic MJ-mediated suppression on VEGF expression, migration, invasion and angiogenesis of gastric cancer cells. Human gastric cancer cell lines SGC-7901 and MKN-45 were transfected by MMP-14 expression vector for 72 hrs, and incubated with sub-cytotoxic MJ for 24 hrs. A and B , western blot and real-time quantitative RT-PCR indicated that transfection of SGC-7901 and MKN-45 cells with MMP-14 construct rescued the MJ-attenuated expression of MMP-14 and VEGF, when compared to those transfected with empty vector (mock) and treated with solvent. C , in scratch migration assay, over-expression of MMP-14 promoted the migration of SGC-7901 and MKN-45 cells, and rescued the 0.2 mM MJ-induced inhibition on the migration of cancer cells, when compared to that of solvent-treated mock cells. D , transwell analysis indicated that restoration of MMP-14 expression rescued the SGC-7901 and MKN-45 cells from 0.2 mM MJ-induced suppression of invasiveness, when compared to that of solvent-treated mock cells. E , restoration of MMP-14 expression in SGC-7901 and MKN-45 cells rescued the 0.2 mM MJ-induced suppression of angiogenesis, when compared to that of solvent-treated mock cells. The symbols (* and △) indicate a significant decrease and a significant increase from solvent-treated mock cells, respectively.
Figure Legend Snippet: Restoration of MMP-14 rescued sub-cytotoxic MJ-mediated suppression on VEGF expression, migration, invasion and angiogenesis of gastric cancer cells. Human gastric cancer cell lines SGC-7901 and MKN-45 were transfected by MMP-14 expression vector for 72 hrs, and incubated with sub-cytotoxic MJ for 24 hrs. A and B , western blot and real-time quantitative RT-PCR indicated that transfection of SGC-7901 and MKN-45 cells with MMP-14 construct rescued the MJ-attenuated expression of MMP-14 and VEGF, when compared to those transfected with empty vector (mock) and treated with solvent. C , in scratch migration assay, over-expression of MMP-14 promoted the migration of SGC-7901 and MKN-45 cells, and rescued the 0.2 mM MJ-induced inhibition on the migration of cancer cells, when compared to that of solvent-treated mock cells. D , transwell analysis indicated that restoration of MMP-14 expression rescued the SGC-7901 and MKN-45 cells from 0.2 mM MJ-induced suppression of invasiveness, when compared to that of solvent-treated mock cells. E , restoration of MMP-14 expression in SGC-7901 and MKN-45 cells rescued the 0.2 mM MJ-induced suppression of angiogenesis, when compared to that of solvent-treated mock cells. The symbols (* and △) indicate a significant decrease and a significant increase from solvent-treated mock cells, respectively.

Techniques Used: Expressing, Migration, Transfection, Plasmid Preparation, Incubation, Western Blot, Quantitative RT-PCR, Construct, Over Expression, Inhibition

27) Product Images from "Application of peripheral-blood-derived endothelial progenitor cell for treating ischemia-reperfusion injury and infarction: a preclinical study in rat models"

Article Title: Application of peripheral-blood-derived endothelial progenitor cell for treating ischemia-reperfusion injury and infarction: a preclinical study in rat models

Journal: Journal of Cardiothoracic Surgery

doi: 10.1186/1749-8090-8-33

EPC treatment induced cardioprotection related proteins expression. Protein expression of platelet-derived growth factor (PDGF: A ), vascular endothelial growth factor (VEGF: B ), fetal liver kinase-1 (Flk-1: C ), fibroblast growth factor-17 (FGF-17: D ), fibroblast growth factor receptor-2 (FGFR-2: E ), Tbx-18 ( F ), and β-Catenin ( G ) in heart tissue from rats 24 h after myocardial infarction. Rats were treated with endothelial progenitor cells (EPCs) or were untreated (controls). Protein expression levels were determined by Western blot using heart tissue harvested from non-infarct areas. Density units were normalized to the GAPDH loading control. * P
Figure Legend Snippet: EPC treatment induced cardioprotection related proteins expression. Protein expression of platelet-derived growth factor (PDGF: A ), vascular endothelial growth factor (VEGF: B ), fetal liver kinase-1 (Flk-1: C ), fibroblast growth factor-17 (FGF-17: D ), fibroblast growth factor receptor-2 (FGFR-2: E ), Tbx-18 ( F ), and β-Catenin ( G ) in heart tissue from rats 24 h after myocardial infarction. Rats were treated with endothelial progenitor cells (EPCs) or were untreated (controls). Protein expression levels were determined by Western blot using heart tissue harvested from non-infarct areas. Density units were normalized to the GAPDH loading control. * P

Techniques Used: Expressing, Derivative Assay, Western Blot

28) Product Images from "Epidermal Stem Cells Cultured on Collagen-Modified Chitin Membrane Induce In Situ Tissue Regeneration of Full-Thickness Skin Defects in Mice"

Article Title: Epidermal Stem Cells Cultured on Collagen-Modified Chitin Membrane Induce In Situ Tissue Regeneration of Full-Thickness Skin Defects in Mice

Journal: PLoS ONE

doi: 10.1371/journal.pone.0087557

Early-stage markers of protein level and m-RNA level of epidermal stem cells (ESCs). (A) Western blot analysis to detect marked protein. (B) Quantitative real time polymerase chain reaction (RT-PCR) analysis of m-RNA levels: (a) CD-29 increased at d3; (b) p63 was maintained at a relatively high level; (c) VEGF increased at d3–5; (d) CK15 was maintained at a relatively high level; (e) CK-19 was still highly expressed at d28; (f) miR-203 was significantly low at d28 compared to the control group, * P
Figure Legend Snippet: Early-stage markers of protein level and m-RNA level of epidermal stem cells (ESCs). (A) Western blot analysis to detect marked protein. (B) Quantitative real time polymerase chain reaction (RT-PCR) analysis of m-RNA levels: (a) CD-29 increased at d3; (b) p63 was maintained at a relatively high level; (c) VEGF increased at d3–5; (d) CK15 was maintained at a relatively high level; (e) CK-19 was still highly expressed at d28; (f) miR-203 was significantly low at d28 compared to the control group, * P

Techniques Used: Western Blot, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

29) Product Images from "Transplantation of bradykinin-preconditioned human endothelial progenitor cells improves cardiac function via enhanced Akt/eNOS phosphorylation and angiogenesis"

Article Title: Transplantation of bradykinin-preconditioned human endothelial progenitor cells improves cardiac function via enhanced Akt/eNOS phosphorylation and angiogenesis

Journal: American Journal of Translational Research

doi:

Effects of BK-PC-hEPC transplantation on Akt, eNOS, VEGF expression, as well as NO production. A and B. Western blots analysis to identify Akt and eNOS expressions. C. NO production in myocardial tissue. D. Western blot analysis for VEGF expression. Representative
Figure Legend Snippet: Effects of BK-PC-hEPC transplantation on Akt, eNOS, VEGF expression, as well as NO production. A and B. Western blots analysis to identify Akt and eNOS expressions. C. NO production in myocardial tissue. D. Western blot analysis for VEGF expression. Representative

Techniques Used: Transplantation Assay, Expressing, Western Blot

30) Product Images from "The Transcriptional Activator Hypoxia Inducible Factor 2 (HIF-2/EPAS-1) Regulates the Oxygen-Dependent Expression of Erythropoietin in Cortical Astrocytes"

Article Title: The Transcriptional Activator Hypoxia Inducible Factor 2 (HIF-2/EPAS-1) Regulates the Oxygen-Dependent Expression of Erythropoietin in Cortical Astrocytes

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.2838-06.2006

Endogenous HIF-2α protein occupies the HREs of Epo in primary astrocytes as shown by ChIP assay. Anti-HIF-1α and anti-HIF-2α antibodies were used to precipitate the HIF-α proteins in nuclear extracts of cross-linked hypoxic HIF-1α F/F and HIF-1α Δ/Δ cells (0.5% O 2 , 0–24 h). Coprecipitated DNA fragments were detected using PCR with primers specific for HREs of Epo , VEGF , LDH , and Glut-1 . IP with anti-ARNT2 was performed in hypoxic HIF-1α F/F astrocytes as a positive control (+). HIF-1α Δ/Δ astrocytes were generated by Ad-Cre infection as described in Materials and Methods.
Figure Legend Snippet: Endogenous HIF-2α protein occupies the HREs of Epo in primary astrocytes as shown by ChIP assay. Anti-HIF-1α and anti-HIF-2α antibodies were used to precipitate the HIF-α proteins in nuclear extracts of cross-linked hypoxic HIF-1α F/F and HIF-1α Δ/Δ cells (0.5% O 2 , 0–24 h). Coprecipitated DNA fragments were detected using PCR with primers specific for HREs of Epo , VEGF , LDH , and Glut-1 . IP with anti-ARNT2 was performed in hypoxic HIF-1α F/F astrocytes as a positive control (+). HIF-1α Δ/Δ astrocytes were generated by Ad-Cre infection as described in Materials and Methods.

Techniques Used: Chromatin Immunoprecipitation, Polymerase Chain Reaction, Positive Control, Generated, Infection

Cre recombinase-mediated deletion of the HIF-1α allele in cultured astrocytes derived from double-floxed HIF-1α transgenic mice. A , PCR analysis of the occurrence of Cre-mediated deletion of HIF-1α in astrocytes isolated from HIF-1α F/F mice. At various time points after infection of astrocytes with Ad-Cre, DNA was extracted and PCR was performed with the primers indicated in the diagram. The amplified fragments correspond to the floxed HIF-1α allele (HIF-1α F ) and the HIF1α-deleted allele (HIF-1α Δ ). B , HIF-1α F/F astrocytes were infected with Ad-GFP or Ad-Cre and 5 d later exposed to normoxia (N) or hypoxia (H) (0.5% O 2 for 24 h). Total RNA was extracted for RT-PCR analysis of Cre recombinase, HIF-1α, HIF-2α, and glyceraldehyde-3-phosphate dehydrogenase (loading control) expression. In parallel experiments, nuclear extracts were prepared for immunoblot analysis of HIF-1α, HIF-2α, and α-tubulin (loading control). C , HIF-1α F/F cortical astrocytes were subjected to the same experimental conditions as described in B , and real-time RT-PCR was performed for EPO, LDH, VEGF, and β-actin (loading control). Data are expressed as the mean ± SD from three to four independent experiments. * p
Figure Legend Snippet: Cre recombinase-mediated deletion of the HIF-1α allele in cultured astrocytes derived from double-floxed HIF-1α transgenic mice. A , PCR analysis of the occurrence of Cre-mediated deletion of HIF-1α in astrocytes isolated from HIF-1α F/F mice. At various time points after infection of astrocytes with Ad-Cre, DNA was extracted and PCR was performed with the primers indicated in the diagram. The amplified fragments correspond to the floxed HIF-1α allele (HIF-1α F ) and the HIF1α-deleted allele (HIF-1α Δ ). B , HIF-1α F/F astrocytes were infected with Ad-GFP or Ad-Cre and 5 d later exposed to normoxia (N) or hypoxia (H) (0.5% O 2 for 24 h). Total RNA was extracted for RT-PCR analysis of Cre recombinase, HIF-1α, HIF-2α, and glyceraldehyde-3-phosphate dehydrogenase (loading control) expression. In parallel experiments, nuclear extracts were prepared for immunoblot analysis of HIF-1α, HIF-2α, and α-tubulin (loading control). C , HIF-1α F/F cortical astrocytes were subjected to the same experimental conditions as described in B , and real-time RT-PCR was performed for EPO, LDH, VEGF, and β-actin (loading control). Data are expressed as the mean ± SD from three to four independent experiments. * p

Techniques Used: Cell Culture, Derivative Assay, Transgenic Assay, Mouse Assay, Polymerase Chain Reaction, Isolation, Infection, Amplification, Reverse Transcription Polymerase Chain Reaction, Expressing, Quantitative RT-PCR

31) Product Images from "The somatostatin analogue octreotide inhibits angiogenesis in the earliest, but not in advanced, stages of portal hypertension in rats"

Article Title: The somatostatin analogue octreotide inhibits angiogenesis in the earliest, but not in advanced, stages of portal hypertension in rats

Journal: Journal of Cellular and Molecular Medicine

doi: 10.1111/j.1582-4934.2008.00218.x

Expression of VEGF and CD31 in the intestine of portal hypertensive rats treated with octreotide or vehicle during 4 or 7 days after induction of portal hypertension by partial portal vein ligation (PPVL). Representative blots for VEGF, CD31 and the housekeeping protein GAPDH are shown at the left and quantification of expression normalized to GAPDH and expressed as arbitrary units is shown at the right.
Figure Legend Snippet: Expression of VEGF and CD31 in the intestine of portal hypertensive rats treated with octreotide or vehicle during 4 or 7 days after induction of portal hypertension by partial portal vein ligation (PPVL). Representative blots for VEGF, CD31 and the housekeeping protein GAPDH are shown at the left and quantification of expression normalized to GAPDH and expressed as arbitrary units is shown at the right.

Techniques Used: Expressing, Ligation

32) Product Images from "Nobiletin alleviates endometriosis via down-regulating NF-κB activity in endometriosis mouse model"

Article Title: Nobiletin alleviates endometriosis via down-regulating NF-κB activity in endometriosis mouse model

Journal: Bioscience Reports

doi: 10.1042/BSR20180470

Effects of nobiletin on the expression of proliferation and angiogenesis relevant genes in ectopic endometrium in vivo ( A ) Immunohistochemistry analysis of PCNA, VEGF, and E-cadherin. ( B ) The staining levels of PCNA, VEGF, and E-cadherin in different treatment groups. Abbreviations used in the figure: EM, endometriosis mice without treatment; N10, endometriosis mice treated 10 mg/kg/day; N20, endometriosis mice treated 20 mg/kg/day; N =6 mice per group. Values are expressed as means ± S.E.M. of three independent experiments; ** P
Figure Legend Snippet: Effects of nobiletin on the expression of proliferation and angiogenesis relevant genes in ectopic endometrium in vivo ( A ) Immunohistochemistry analysis of PCNA, VEGF, and E-cadherin. ( B ) The staining levels of PCNA, VEGF, and E-cadherin in different treatment groups. Abbreviations used in the figure: EM, endometriosis mice without treatment; N10, endometriosis mice treated 10 mg/kg/day; N20, endometriosis mice treated 20 mg/kg/day; N =6 mice per group. Values are expressed as means ± S.E.M. of three independent experiments; ** P

Techniques Used: Expressing, In Vivo, Immunohistochemistry, Staining, Mouse Assay

33) Product Images from "Rapamycin Attenuates Splenomegaly in both Intrahepatic and Prehepatic Portal Hypertensive Rats by Blocking mTOR Signaling Pathway"

Article Title: Rapamycin Attenuates Splenomegaly in both Intrahepatic and Prehepatic Portal Hypertensive Rats by Blocking mTOR Signaling Pathway

Journal: PLoS ONE

doi: 10.1371/journal.pone.0141159

Effects of rapamycin on angiogenesis of splenic red pulp in portal hypertensive rats. (A) Representative images of western blot for VEGF and α-SMA. (B) Quantification of VEGF and α-SMA protein expression relative to GAPDH. (C) Representative histological images of spleen tissues immunostained for α-SMA and Ki67 (original magnification ×40). Arrowheads point to α-SMA- and Ki67-positive cells. (D) Quantitative analysis of α-SMA and Ki67-positive cells area. *: p
Figure Legend Snippet: Effects of rapamycin on angiogenesis of splenic red pulp in portal hypertensive rats. (A) Representative images of western blot for VEGF and α-SMA. (B) Quantification of VEGF and α-SMA protein expression relative to GAPDH. (C) Representative histological images of spleen tissues immunostained for α-SMA and Ki67 (original magnification ×40). Arrowheads point to α-SMA- and Ki67-positive cells. (D) Quantitative analysis of α-SMA and Ki67-positive cells area. *: p

Techniques Used: Western Blot, Expressing

34) Product Images from "Long-Duration Three-Dimensional Spheroid Culture Promotes Angiogenic Activities of Adipose-Derived Mesenchymal Stem Cells"

Article Title: Long-Duration Three-Dimensional Spheroid Culture Promotes Angiogenic Activities of Adipose-Derived Mesenchymal Stem Cells

Journal: Biomolecules & Therapeutics

doi: 10.4062/biomolther.2015.146

Expression of hypoxia-induced angiogenic cytokines in MSC spheroids. (A) Western blot analysis for hypoxic inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), stromal cell derived factor (SDF), and hepatocyte growth factor (HGF) in monolayer MSCs and MSC spheroids cultured for 3, 5, and 7 days. (B-E) Relative expression of HIF-1α, VEGF, SDF, and HGF normalized to that of β-actin. Values represent means ± SEM. ** p
Figure Legend Snippet: Expression of hypoxia-induced angiogenic cytokines in MSC spheroids. (A) Western blot analysis for hypoxic inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), stromal cell derived factor (SDF), and hepatocyte growth factor (HGF) in monolayer MSCs and MSC spheroids cultured for 3, 5, and 7 days. (B-E) Relative expression of HIF-1α, VEGF, SDF, and HGF normalized to that of β-actin. Values represent means ± SEM. ** p

Techniques Used: Expressing, Western Blot, Derivative Assay, Cell Culture

35) Product Images from "RAGE-aptamer Attenuates the Growth and Liver Metastasis of Malignant Melanoma in Nude Mice"

Article Title: RAGE-aptamer Attenuates the Growth and Liver Metastasis of Malignant Melanoma in Nude Mice

Journal: Molecular Medicine

doi: 10.2119/molmed.2017.00099

Effects of RAGE-aptamer on immunostaining of (A) PCNA, (B) cyclin D1, (C) p27, (D) VEGF, (E) CD31, (F) MCP-1 and (G) Mac-3 in G361 tumors. (A–G) Each left panel shows representative photographs of immunostaining. (E) Arrows indicate CD31-positive microvessels. Quantitative data are shown in each middle panel. (B) Cyclin D1 , (C) p27 , (D) VEGF and (F) MCP-1 mRNA levels in G361 tumors are shown in the rightmost panel.
Figure Legend Snippet: Effects of RAGE-aptamer on immunostaining of (A) PCNA, (B) cyclin D1, (C) p27, (D) VEGF, (E) CD31, (F) MCP-1 and (G) Mac-3 in G361 tumors. (A–G) Each left panel shows representative photographs of immunostaining. (E) Arrows indicate CD31-positive microvessels. Quantitative data are shown in each middle panel. (B) Cyclin D1 , (C) p27 , (D) VEGF and (F) MCP-1 mRNA levels in G361 tumors are shown in the rightmost panel.

Techniques Used: Immunostaining

Effects of RAGE-aptamer on (A) ROS generation, (B) proliferation, (C) VEGF , (D) VCAM-1 and (E) MCP-1 mRNA levels in, (F) THP-1 cell adhesion to, and (G) tube formation of, AGE-exposed HUVECs. (A) Superoxide generation was evaluated by the fluorescence intensity of DHE staining. (B) Proliferation was determined by [ 3 H]thymidine incorporation into HUVECs. (C–E) Total RNA was transcribed and amplified by real-time PCR. Data were normalized by the intensity of β-actin–derived signals and related to the value obtained with nonglycated BSA alone. (G) Length of tube-like structures was measured. Upper panels show the representative microphotographs. (A) N = 3 for each group. (B) N = 4 for each group. (C) N = 6 for each group. (D, E) N = 3 for each group. (F) N = 6 for each group. (G) N = 3 for each group. * and **, p
Figure Legend Snippet: Effects of RAGE-aptamer on (A) ROS generation, (B) proliferation, (C) VEGF , (D) VCAM-1 and (E) MCP-1 mRNA levels in, (F) THP-1 cell adhesion to, and (G) tube formation of, AGE-exposed HUVECs. (A) Superoxide generation was evaluated by the fluorescence intensity of DHE staining. (B) Proliferation was determined by [ 3 H]thymidine incorporation into HUVECs. (C–E) Total RNA was transcribed and amplified by real-time PCR. Data were normalized by the intensity of β-actin–derived signals and related to the value obtained with nonglycated BSA alone. (G) Length of tube-like structures was measured. Upper panels show the representative microphotographs. (A) N = 3 for each group. (B) N = 4 for each group. (C) N = 6 for each group. (D, E) N = 3 for each group. (F) N = 6 for each group. (G) N = 3 for each group. * and **, p

Techniques Used: Fluorescence, Staining, Amplification, Real-time Polymerase Chain Reaction, Derivative Assay

Effects of RAGE-aptamer or NAC on (A) ROS generation, (B, C) proliferation, (D, E) cyclin D1 and p27 protein levels, (F, G) VEGF and (H, I) MCP-1 mRNA levels in AGE-exposed G361 melanoma cells. (D, E) Each upper panel shows the bands of Western blot analysis for cyclin D1, p27 and β-actin, respectively. Quantitative data are shown in each lower panel. Data were normalized by the intensity of β-actin–derived signals and related to the value obtained with nonglycated BSA alone. (A) N = 6 for each group. (B, C, E) N = 3 for each group. (D) N = 5 for each group. (F–I) N = 9 for each group. **, p
Figure Legend Snippet: Effects of RAGE-aptamer or NAC on (A) ROS generation, (B, C) proliferation, (D, E) cyclin D1 and p27 protein levels, (F, G) VEGF and (H, I) MCP-1 mRNA levels in AGE-exposed G361 melanoma cells. (D, E) Each upper panel shows the bands of Western blot analysis for cyclin D1, p27 and β-actin, respectively. Quantitative data are shown in each lower panel. Data were normalized by the intensity of β-actin–derived signals and related to the value obtained with nonglycated BSA alone. (A) N = 6 for each group. (B, C, E) N = 3 for each group. (D) N = 5 for each group. (F–I) N = 9 for each group. **, p

Techniques Used: Western Blot, Derivative Assay

36) Product Images from "Mobilization of Endogenous Bone Marrow Derived Endothelial Progenitor Cells and Therapeutic Potential of Parathyroid Hormone after Ischemic Stroke in Mice"

Article Title: Mobilization of Endogenous Bone Marrow Derived Endothelial Progenitor Cells and Therapeutic Potential of Parathyroid Hormone after Ischemic Stroke in Mice

Journal: PLoS ONE

doi: 10.1371/journal.pone.0087284

Effects of PTH on the expression of angiogenic and neurotrophic factors. The expression of key pro-regenerative factors in the peri-infarct region was detected using Western blot analysis. A . Electrophoresis gels show the protein levels of VEGF, BDNF, EPOR, SDF-1, and Tie-1 in the ischemic peri-infarct region in different animals 6 days after stroke. B to F . Densitometry analysis of the expression of BDNF (B), VEGF (C), EPOR (D), SDF-1 (E), and Tie-1 (F). Gray intensity was normalized against β-actin and quantified. PTH treatment significantly enhanced the expression of all these factors shown in A, compared with the stroke-saline group. N = 3 animals for each test; * P
Figure Legend Snippet: Effects of PTH on the expression of angiogenic and neurotrophic factors. The expression of key pro-regenerative factors in the peri-infarct region was detected using Western blot analysis. A . Electrophoresis gels show the protein levels of VEGF, BDNF, EPOR, SDF-1, and Tie-1 in the ischemic peri-infarct region in different animals 6 days after stroke. B to F . Densitometry analysis of the expression of BDNF (B), VEGF (C), EPOR (D), SDF-1 (E), and Tie-1 (F). Gray intensity was normalized against β-actin and quantified. PTH treatment significantly enhanced the expression of all these factors shown in A, compared with the stroke-saline group. N = 3 animals for each test; * P

Techniques Used: Expressing, Western Blot, Electrophoresis

37) Product Images from "Augmented anti-angiogenesis activity of polysulfated heparin-endostatin and polyethylene glycol-endostatin in alkali burn-induced corneal ulcers in rabbits"

Article Title: Augmented anti-angiogenesis activity of polysulfated heparin-endostatin and polyethylene glycol-endostatin in alkali burn-induced corneal ulcers in rabbits

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2015.2602

Vascular endothelial growth factor (VEGF) immunohistochemical assay. (A) Polysulfated heparin-endostatin, (B) polyethylene glycol-endostatin, (C) endostatin and (D) saline groups. The arrows indicate VEGF-positive staining in the luminal structures of
Figure Legend Snippet: Vascular endothelial growth factor (VEGF) immunohistochemical assay. (A) Polysulfated heparin-endostatin, (B) polyethylene glycol-endostatin, (C) endostatin and (D) saline groups. The arrows indicate VEGF-positive staining in the luminal structures of

Techniques Used: Immunohistochemistry, Staining

38) Product Images from "Survivin knockdown and concurrent 4-HPR treatment controlled human glioblastoma in vitro and in vivo"

Article Title: Survivin knockdown and concurrent 4-HPR treatment controlled human glioblastoma in vitro and in vivo

Journal: Neuro-Oncology

doi: 10.1093/neuonc/noq079

Western blotting using subcutaneous solid tumors. (A) Representative Western blots for survivin, PCNA, MMP-9, VEGF, bFGF, and CD31 in the subcutaneous solid tumors of U118MG cells treated with a plasmid encoding survivin shRNA, 4-HPR, or both agents together.
Figure Legend Snippet: Western blotting using subcutaneous solid tumors. (A) Representative Western blots for survivin, PCNA, MMP-9, VEGF, bFGF, and CD31 in the subcutaneous solid tumors of U118MG cells treated with a plasmid encoding survivin shRNA, 4-HPR, or both agents together.

Techniques Used: Western Blot, Plasmid Preparation, shRNA

39) Product Images from "The Impact of Gender on Progression of Renal Disease "

Article Title: The Impact of Gender on Progression of Renal Disease

Journal:

doi:

VEGFR-2 or KDR expression in male and female RK rats. Immunohistochemistry with VEGFR-2 or KDR (see Material and Methods) shows a better preservation of VEGF receptor staining along the ECs of glomerular and peritubular capillaries in female RK rats (
Figure Legend Snippet: VEGFR-2 or KDR expression in male and female RK rats. Immunohistochemistry with VEGFR-2 or KDR (see Material and Methods) shows a better preservation of VEGF receptor staining along the ECs of glomerular and peritubular capillaries in female RK rats (

Techniques Used: Expressing, Immunohistochemistry, Preserving, Staining

40) Product Images from "Expression of activated signal transducer and activator of transcription-3 predicts poor prognosis in cervical squamous-cell carcinoma"

Article Title: Expression of activated signal transducer and activator of transcription-3 predicts poor prognosis in cervical squamous-cell carcinoma

Journal: British Journal of Cancer

doi: 10.1038/sj.bjc.6605212

Expression of Bcl-xL, VEGF, and p-Stat3 in cervical cancer cell line and the effect of Stat3 siRNA ( A ). Relative expression level standardised by β -actin expression level is indicated ( B ). The expression levels of both Bcl-xL and VEGF with Stat3 siRNA were more reduced than that with No Targeting siRNA (NT siRNA).
Figure Legend Snippet: Expression of Bcl-xL, VEGF, and p-Stat3 in cervical cancer cell line and the effect of Stat3 siRNA ( A ). Relative expression level standardised by β -actin expression level is indicated ( B ). The expression levels of both Bcl-xL and VEGF with Stat3 siRNA were more reduced than that with No Targeting siRNA (NT siRNA).

Techniques Used: Expressing

Related Articles

Western Blot:

Article Title: Stress-Induced Activation of Apoptosis Signal-Regulating Kinase 1 Promotes Osteoarthritis
Article Snippet: .. Western blotting was performed as described previously.16 The following list of rabbit antibodies were used: collagen type X (COL-X; Abcam, Cambridge, MA), phosphorylated p38 (Cell Signaling, Danvers, MA), p38 (Invitrogen, Carlsbad, CA), phosphorylated and non-phosphorylated JNK (Millipore, Billerica, MA), phosphorylated and non-phosphorylated ASK (Cell Signaling, Danvers, MA), p65 nuclear factor kappa beta (NFκB), matrix metalloproteinase 13 (MMP13), and vascular endothelial growth factor (VEGF), (Santa Cruz, Dallas, TX); as well as a mouse anti-β-actin (Santa Cruz). .. Immunohistochemistry was used to test for the prevalence of proteins of interest using detection with 3, 3′-diaminobenzidine (DAB) or immunofluorescence, as previously reported ( ).

Article Title: Activation of the Wnt/?-Catenin Signaling Pathway by Mechanical Ventilation Is Associated with Ventilator-Induced Pulmonary Fibrosis in Healthy Lungs
Article Snippet: .. Detection of WNT5A, AXIN2 (Abcam, Cambridge, UK), total β-catenin, MMP-7, cyclin D1, and vascular endothelial growth factor (VEGF) (Santa Cruz Biotechnology, Santa Cruz, CA), non-phospho (Ser33/37/Thr41) β-catenin (Cell Signaling Technology, Massachusetts) were performed in random samples by Western blotting using rabbit polyclonal anti-WNT5A, anti-AXIN2, anti-β-catenin, anti-non-phospho (Ser33/37/Thr41) β-catenin, and anti-MMP-7 antibodies, and a goat anti-rabbit IgG-HRP as secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal antibody anti-cyclin D1 and a rabbit anti-mouse IgG-HRP as secondary antibody (Dako, Glostrup, Denmark), goat polyclonal anti-VEFG and a donkey anti-goat IgG-HRP (Santa Cruz Biotechnology, Santa Cruz, CA). .. Loading control was assessed with a rabbit anti-β-actin antibody (Cell Signaling Technology, Massachusetts).

Incubation:

Article Title: Cripto Enhances Proliferation and Survival of Mesenchymal Stem Cells by Up-Regulating JAK2/STAT3 Pathway in a GRP78-Dependent Manner
Article Snippet: .. After washing with Tris-buffered saline/Tween-20 buffer (0.05% Tween-20, 150 mM NaCl, 10 mM Tris–HCl; pH 7.6), membranes were blocked with 5% bovine serum albumin for 1 h at room temperature and then incubated with primary antibodies against phosphorylated JAK2, STAT3, phosphorylated STAT3, c-Myc, cyclin D1, GRP78, BCL3, cleaved caspase-3, vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), and β-actin (all from Santa Cruz Biotechnology, Dallas, TX, USA). .. After incubation of the membranes with peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology), bands were detected using enhanced chemiluminescence reagents (Amersham Biosciences, Little Chalfont, UK) in a dark room.

Immunohistochemistry:

Article Title: Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Precursor Cells
Article Snippet: .. The sections were stained according to the standard immunohistochemistry/immunofluorescence procedure in 0.1 M PB for 48 hr., The following antigens were stained: Stromal cell–derived factor (SDF)-1 (1:50; Santa Cruz Biotechnology Inc., Dallas, TX), CXC chemokine receptor (CXCR) 4 (1:100; Santa Cruz Biotechnology Inc.), vascular endothelial growth factor (VEGF) (1:100; Santa Cruz Biotechnology Inc.), Reelin (1:50; Santa Cruz Biotechnology Inc.), glial fibrillary acidic protein (GFAP) (1:100; Santa Cruz Biotechnology Inc.), microtubule-associated protein 2 (MAP-2) (1:150; Cell Signaling Technology, Beverly, MA) doublecortin (DCX) (1:100; Santa Cruz Biotechnology Inc.), and BrdU (1:100; Abcam, Cambridge, MA). .. Sections were incubated with primary antibodies overnight at 4°C in phosphate-buffered saline (PBS) with 5% donkey or goat serum and 0.5% Triton X-100.

Staining:

Article Title: Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Precursor Cells
Article Snippet: .. The sections were stained according to the standard immunohistochemistry/immunofluorescence procedure in 0.1 M PB for 48 hr., The following antigens were stained: Stromal cell–derived factor (SDF)-1 (1:50; Santa Cruz Biotechnology Inc., Dallas, TX), CXC chemokine receptor (CXCR) 4 (1:100; Santa Cruz Biotechnology Inc.), vascular endothelial growth factor (VEGF) (1:100; Santa Cruz Biotechnology Inc.), Reelin (1:50; Santa Cruz Biotechnology Inc.), glial fibrillary acidic protein (GFAP) (1:100; Santa Cruz Biotechnology Inc.), microtubule-associated protein 2 (MAP-2) (1:150; Cell Signaling Technology, Beverly, MA) doublecortin (DCX) (1:100; Santa Cruz Biotechnology Inc.), and BrdU (1:100; Abcam, Cambridge, MA). .. Sections were incubated with primary antibodies overnight at 4°C in phosphate-buffered saline (PBS) with 5% donkey or goat serum and 0.5% Triton X-100.

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  • 93
    Santa Cruz Biotechnology rabbit anti vascular endothelial growth factor receptor 3
    Effect of pioglitazone on the expression of F4/80 and VEGFR3  in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.
    Rabbit Anti Vascular Endothelial Growth Factor Receptor 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti vegf c
    sPLA 2 s induce the release of <t>VEGF-A</t> and <t>VEGF-C</t> from HLMs. A and B , HLMs were incubated (37°C, 24 h) with RPMI 1640 alone (Control) or with the indicated concentrations of hGIIA, hGX, or LPS. VEGF-A ( A ) and VEGF-C ( B ) release was determined by ELISA. Data are mean ± SEM of four experiments. * p
    Anti Vegf C, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse monoclonal anti vegf igg
    Immunohistochemical localisation of <t>VEGF‐C</t> in paraffin‐embedded uterine sections of day 4 ( a – f ) and ( g – k ) of gestation. The growth factor strongly expressed in endometrial surface epithelium (ESE) and glandular epithelium (GE), and moderately in stromal cells in control females ( a – c ) on day 4 of gestation. The myometrium (M) and perimetrium (P) in control females showed moderate and strong expression of VEGF‐C, respectively. CBE administration ( d – f ) caused diffused expression in ESE, in stromal cells, and in the endometrial glands. Control females’ uteri on day 5 ( g – i ) of gestation showed strong expression in ESE, GE, certain regions of sub‐epithelial stromal (S) cells and blood vessels (BV). Administration of CBE caused structural aberration in uteri of day 5 gestation ( j – k ). Expression of VEGF‐C was diffused in the desquamated endometrial surface epithelium (DESE) and degenerated glandular epithelium (DGE). Expression was lesser in the vacuolated (V) epithelial cells. The expression of growth factor was indicated by arrow ( thick arrow ). The negative control (L) was immunostained with normal <t>IgG.</t> Original magnification ( a – l ) ×40
    Mouse Monoclonal Anti Vegf Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti vegf
    Effect of cyclin A1 expression on tumor invasion is associated with its effect on <t>VEGF</t> expression in MCF-7 cells. (A) Evaluation of cyclin A1 and VEGF expression in metastatic lesions from lymph nodes from patients with breast cancer metastasis using immunohistochemical analysis. Representative pictures show the cancer cells are strongly positive to cyclin A1 and VEGF expression. Upper panels represent cores at 20x magnificantion and lower panels represent the higher magnification (40x) of the selected areas. (B) MCF-7 cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP vectors were applied on the Matrigel-coated invasion chamber and were assessed after 48 or 72 hours. Data in graphs are the mean ± SD represents two independent experiments, each performed in duplicates. P value is indicated. (C) MDA-MB-231 cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP were applied on the Matrigel-coated invasion chamber and were analyzed after 48 or 72 hours. Data in graphs are the mean of two independent experiments, each performed in duplicate, p=0.002 for 48 h and p=0.02 for 72 h. (D) Cell cycle distribution of the cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. Data in graphs are the mean ± SD represents three independent experiments from flow cytometry analysis. The percentage of cells at onset of each cell cycle phase is indicated. (E) Western blot analysis shows the levels of cyclin D1 and <t>CDK1</t> protein expression in the cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. (F) Representative picture shows the VEGF mRNA expression in the cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP (upper panel). Quantification of VEGF mRNA level in the samples is indicated. P value is shown (lower panel). (G) ELISA assay of VEGF secretion in the cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. Mean ± SD represents three independent experiments (lower panel). Breast cancer cell lines used for these studies are T47D, MCF-7 and MDA-MB231 as indicated.
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    Effect of pioglitazone on the expression of F4/80 and VEGFR3  in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.

    Journal: Molecular Medicine Reports

    Article Title: Pioglitazone increases VEGFR3 expression and promotes activation of M2 macrophages via the peroxisome proliferator-activated receptor γ

    doi: 10.3892/mmr.2019.9945

    Figure Lengend Snippet: Effect of pioglitazone on the expression of F4/80 and VEGFR3 in vivo . Immunofluorescence analysis of F4/80 and VEGFR3 co-localization (white arrows) in kidney sections from mice in the sham, UUO and UUO + pioglitazone groups. Pio, pioglitazone; UUO, unilateral ureteral obstruction; VEGFR3, vascular endothelial growth factor receptor 3.

    Article Snippet: Then they were incubated overnight at 4°C with rabbit anti-arginase1 (Arg1; cat. no. sc-20150; 1:400; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), rabbit anti-vascular endothelial growth factor receptor 3 (VEGFR3; cat. no. sc-321; 1:400; Santa Cruz Biotechnology, Inc.), mouse anti-inducible nitric oxide synthase (iNOS; cat. no. sc-7271; 1:200; Santa Cruz Biotechnology, Inc.) and rabbit anti-α-tubulin (1:5,000; cat. no. ab18251; Abcam, Cambridge, MA, USA).

    Techniques: Expressing, In Vivo, Immunofluorescence, Mouse Assay

    sPLA 2 s induce the release of VEGF-A and VEGF-C from HLMs. A and B , HLMs were incubated (37°C, 24 h) with RPMI 1640 alone (Control) or with the indicated concentrations of hGIIA, hGX, or LPS. VEGF-A ( A ) and VEGF-C ( B ) release was determined by ELISA. Data are mean ± SEM of four experiments. * p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Production of Vascular Endothelial Growth Factors from Human Lung Macrophages Induced by Group IIA and Group X Secreted Phospholipases A2

    doi: 10.4049/jimmunol.0902501

    Figure Lengend Snippet: sPLA 2 s induce the release of VEGF-A and VEGF-C from HLMs. A and B , HLMs were incubated (37°C, 24 h) with RPMI 1640 alone (Control) or with the indicated concentrations of hGIIA, hGX, or LPS. VEGF-A ( A ) and VEGF-C ( B ) release was determined by ELISA. Data are mean ± SEM of four experiments. * p

    Article Snippet: The following were purchased: LPS (from Escherichia coli serotype 026:B6), 5′-(N-ethylcarboxamido)-adenosine (NECA), 2- p -(2-Carboxyethyl)phenethylamino-5′-N-ethylcarboxamidoadenosine , 2-Chloro-N6 -(3-iodobenzyl)-adenosine-5′-N-methyluronamide (Cl-IB-MECA), Pipes, BSA, Percoll, l -glutamine, antibiotic-antimycotic solution (10,000 UI/ml penicillin, 10 mg/ml streptomycin, and 25 μg/ml amphotericin B), Triton X-100, Polymyxin B (Sigma-Aldrich, St. Louis, MO); RPMI 1640, FCS, and guanidine hydrochloride (MP Biomedicals Europe, Illkirch, France); rabbit polyclonal anti–VEGF-B (H-70), anti–VEGF-C (H-190), and anti–VEGF-D (H-144) Abs, goat polyclonal anti-PlGF (C-20) and anti-GAPDH (V-18) Abs, normal rabbit and goat IgG Abs, HRP-conjugated donkey anti-rabbit and anti-goat IgG Abs (Santa Cruz Biotechnology, Santa Cruz, CA); goat polyclonal anti–VEGF-A Ab, human recombinant VEGF-A165 , and VEGF-A121 (R & D System, Minneapolis, MN); SB203580 (Calbiochem, La Jolla, CA); PD98059 (Cell Signaling, Beverly, MA).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay

    HLMs constitutively express different forms of VEGF. A , Expression of VEGF mRNAs. RNA extraction from resting HLMs and RT-PCR was performed as described under Materials and Methods . Specific RT-PCR amplification products for VEGFA (isoforms 189, 165, and 121), VEGFB (isoforms 186 and 167), VEGFC, VEGFD, PlGF , and GAPDH were separated on 2% agarose gel, stained with ethidium bromide, and visualized with an image analysis system. The experiment shown is representative of three separate experiments. B , Detection of VEGF proteins. HLM protein extracts (40 μg per sample) were immunoblotted with anti–VEGF-A (gel I and II), anti-PlGF (gel III), anti–VEGF-B (gel IV), anti–VEGF-C (gel V), and anti–VEGF-D (gel VI) Abs. rhVEGF-A 165 , MCF-7 cells, EBNA expressing PlGF-1, RAW 264.7 cells were used as positive controls. Stripped membranes were reprobed with anti-GAPDH Ab to confirm equal protein content of each sample. Each Western blot shown is representative of three separate experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Production of Vascular Endothelial Growth Factors from Human Lung Macrophages Induced by Group IIA and Group X Secreted Phospholipases A2

    doi: 10.4049/jimmunol.0902501

    Figure Lengend Snippet: HLMs constitutively express different forms of VEGF. A , Expression of VEGF mRNAs. RNA extraction from resting HLMs and RT-PCR was performed as described under Materials and Methods . Specific RT-PCR amplification products for VEGFA (isoforms 189, 165, and 121), VEGFB (isoforms 186 and 167), VEGFC, VEGFD, PlGF , and GAPDH were separated on 2% agarose gel, stained with ethidium bromide, and visualized with an image analysis system. The experiment shown is representative of three separate experiments. B , Detection of VEGF proteins. HLM protein extracts (40 μg per sample) were immunoblotted with anti–VEGF-A (gel I and II), anti-PlGF (gel III), anti–VEGF-B (gel IV), anti–VEGF-C (gel V), and anti–VEGF-D (gel VI) Abs. rhVEGF-A 165 , MCF-7 cells, EBNA expressing PlGF-1, RAW 264.7 cells were used as positive controls. Stripped membranes were reprobed with anti-GAPDH Ab to confirm equal protein content of each sample. Each Western blot shown is representative of three separate experiments.

    Article Snippet: The following were purchased: LPS (from Escherichia coli serotype 026:B6), 5′-(N-ethylcarboxamido)-adenosine (NECA), 2- p -(2-Carboxyethyl)phenethylamino-5′-N-ethylcarboxamidoadenosine , 2-Chloro-N6 -(3-iodobenzyl)-adenosine-5′-N-methyluronamide (Cl-IB-MECA), Pipes, BSA, Percoll, l -glutamine, antibiotic-antimycotic solution (10,000 UI/ml penicillin, 10 mg/ml streptomycin, and 25 μg/ml amphotericin B), Triton X-100, Polymyxin B (Sigma-Aldrich, St. Louis, MO); RPMI 1640, FCS, and guanidine hydrochloride (MP Biomedicals Europe, Illkirch, France); rabbit polyclonal anti–VEGF-B (H-70), anti–VEGF-C (H-190), and anti–VEGF-D (H-144) Abs, goat polyclonal anti-PlGF (C-20) and anti-GAPDH (V-18) Abs, normal rabbit and goat IgG Abs, HRP-conjugated donkey anti-rabbit and anti-goat IgG Abs (Santa Cruz Biotechnology, Santa Cruz, CA); goat polyclonal anti–VEGF-A Ab, human recombinant VEGF-A165 , and VEGF-A121 (R & D System, Minneapolis, MN); SB203580 (Calbiochem, La Jolla, CA); PD98059 (Cell Signaling, Beverly, MA).

    Techniques: Expressing, RNA Extraction, Reverse Transcription Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Staining, Western Blot

    Immunohistochemical localisation of VEGF‐C in paraffin‐embedded uterine sections of day 4 ( a – f ) and ( g – k ) of gestation. The growth factor strongly expressed in endometrial surface epithelium (ESE) and glandular epithelium (GE), and moderately in stromal cells in control females ( a – c ) on day 4 of gestation. The myometrium (M) and perimetrium (P) in control females showed moderate and strong expression of VEGF‐C, respectively. CBE administration ( d – f ) caused diffused expression in ESE, in stromal cells, and in the endometrial glands. Control females’ uteri on day 5 ( g – i ) of gestation showed strong expression in ESE, GE, certain regions of sub‐epithelial stromal (S) cells and blood vessels (BV). Administration of CBE caused structural aberration in uteri of day 5 gestation ( j – k ). Expression of VEGF‐C was diffused in the desquamated endometrial surface epithelium (DESE) and degenerated glandular epithelium (DGE). Expression was lesser in the vacuolated (V) epithelial cells. The expression of growth factor was indicated by arrow ( thick arrow ). The negative control (L) was immunostained with normal IgG. Original magnification ( a – l ) ×40

    Journal: Reproductive Medicine and Biology

    Article Title: Crude bark extract of Dysozylum alliarium induces alteration in histological structures and VEGF‐C expression in uterus during days 4–7 of gestation in albino rat

    doi: 10.1007/s12522-012-0143-8

    Figure Lengend Snippet: Immunohistochemical localisation of VEGF‐C in paraffin‐embedded uterine sections of day 4 ( a – f ) and ( g – k ) of gestation. The growth factor strongly expressed in endometrial surface epithelium (ESE) and glandular epithelium (GE), and moderately in stromal cells in control females ( a – c ) on day 4 of gestation. The myometrium (M) and perimetrium (P) in control females showed moderate and strong expression of VEGF‐C, respectively. CBE administration ( d – f ) caused diffused expression in ESE, in stromal cells, and in the endometrial glands. Control females’ uteri on day 5 ( g – i ) of gestation showed strong expression in ESE, GE, certain regions of sub‐epithelial stromal (S) cells and blood vessels (BV). Administration of CBE caused structural aberration in uteri of day 5 gestation ( j – k ). Expression of VEGF‐C was diffused in the desquamated endometrial surface epithelium (DESE) and degenerated glandular epithelium (DGE). Expression was lesser in the vacuolated (V) epithelial cells. The expression of growth factor was indicated by arrow ( thick arrow ). The negative control (L) was immunostained with normal IgG. Original magnification ( a – l ) ×40

    Article Snippet: Cat No.Optitran BA‐S 85, 200 × 3 mm) were blocked for one and half hour in 3 % BSA in Tris‐buffered saline (Santa Cruz Biotechnology) and then incubated with mouse monoclonal anti VEGF IgG at 1.5 μg/ml (Santa Cruz Biotechnology, Santa Cruz, USA Cat No. sc‐7269) concentration in TBS–BSA overnight at 4 °C.

    Techniques: Immunohistochemistry, Expressing, Negative Control

    Effect of cyclin A1 expression on tumor invasion is associated with its effect on VEGF expression in MCF-7 cells. (A) Evaluation of cyclin A1 and VEGF expression in metastatic lesions from lymph nodes from patients with breast cancer metastasis using immunohistochemical analysis. Representative pictures show the cancer cells are strongly positive to cyclin A1 and VEGF expression. Upper panels represent cores at 20x magnificantion and lower panels represent the higher magnification (40x) of the selected areas. (B) MCF-7 cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP vectors were applied on the Matrigel-coated invasion chamber and were assessed after 48 or 72 hours. Data in graphs are the mean ± SD represents two independent experiments, each performed in duplicates. P value is indicated. (C) MDA-MB-231 cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP were applied on the Matrigel-coated invasion chamber and were analyzed after 48 or 72 hours. Data in graphs are the mean of two independent experiments, each performed in duplicate, p=0.002 for 48 h and p=0.02 for 72 h. (D) Cell cycle distribution of the cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. Data in graphs are the mean ± SD represents three independent experiments from flow cytometry analysis. The percentage of cells at onset of each cell cycle phase is indicated. (E) Western blot analysis shows the levels of cyclin D1 and CDK1 protein expression in the cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. (F) Representative picture shows the VEGF mRNA expression in the cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP (upper panel). Quantification of VEGF mRNA level in the samples is indicated. P value is shown (lower panel). (G) ELISA assay of VEGF secretion in the cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. Mean ± SD represents three independent experiments (lower panel). Breast cancer cell lines used for these studies are T47D, MCF-7 and MDA-MB231 as indicated.

    Journal: PLoS ONE

    Article Title: Cyclin A1 Modulates the Expression of Vascular Endothelial Growth Factor and Promotes Hormone-Dependent Growth and Angiogenesis of Breast Cancer

    doi: 10.1371/journal.pone.0072210

    Figure Lengend Snippet: Effect of cyclin A1 expression on tumor invasion is associated with its effect on VEGF expression in MCF-7 cells. (A) Evaluation of cyclin A1 and VEGF expression in metastatic lesions from lymph nodes from patients with breast cancer metastasis using immunohistochemical analysis. Representative pictures show the cancer cells are strongly positive to cyclin A1 and VEGF expression. Upper panels represent cores at 20x magnificantion and lower panels represent the higher magnification (40x) of the selected areas. (B) MCF-7 cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP vectors were applied on the Matrigel-coated invasion chamber and were assessed after 48 or 72 hours. Data in graphs are the mean ± SD represents two independent experiments, each performed in duplicates. P value is indicated. (C) MDA-MB-231 cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP were applied on the Matrigel-coated invasion chamber and were analyzed after 48 or 72 hours. Data in graphs are the mean of two independent experiments, each performed in duplicate, p=0.002 for 48 h and p=0.02 for 72 h. (D) Cell cycle distribution of the cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. Data in graphs are the mean ± SD represents three independent experiments from flow cytometry analysis. The percentage of cells at onset of each cell cycle phase is indicated. (E) Western blot analysis shows the levels of cyclin D1 and CDK1 protein expression in the cells that were transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. (F) Representative picture shows the VEGF mRNA expression in the cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP (upper panel). Quantification of VEGF mRNA level in the samples is indicated. P value is shown (lower panel). (G) ELISA assay of VEGF secretion in the cells transfected with cyclin A1pCMS-EGFP or pCMS-EGFP. Mean ± SD represents three independent experiments (lower panel). Breast cancer cell lines used for these studies are T47D, MCF-7 and MDA-MB231 as indicated.

    Article Snippet: Polyclonal human anti-CD31 and CDK1 (BD Pharmingen), anti-β-actin, anti-VEGF, anti-VEGF-R1 and VEGF-R2 (Santa Cruz Biotechnology, CA), anti-ER-α, Ki67 (Dako, Golstrup, Denmark) and anti-cyclin D1 (Cell Signaling Technology Inc, Danvers, MA).

    Techniques: Expressing, Immunohistochemistry, Transfection, Multiple Displacement Amplification, Flow Cytometry, Cytometry, Western Blot, Enzyme-linked Immunosorbent Assay

    Long-term effect of elevated level of cyclin A1 on growth and angiogenesis phenotype of xenograft tumors in mice. MCF-7 cells stable expressing pcDNA–cyclin A1 or pcDNA vectors were subcutaneous implanted into female nude mice with E2 supplementation. (A, B) Representative microphotographs of xenograft tumor sections stained with Haematoxylin and Eosin are shown. (C, D) Representative pictures show the xenograft tumors stained with antibody against human CD31, the CD31 positive vessels are indicated. The control tumor “control-pcDNA” and cyclin A1 expressing tumors “cyclin A1-pcDNA” are indicated. (E) Growth curves of the two groups of xenograft tumors are indicated. The time is indicated in x-axis and tumor volume in mm 3 is indicated in y-axis. (F) Quantification of the tumor vascularizations in cyclin A1 expressing xenograft tumors “cyclin A1-pcDNA” and in control xenograft tumors “control-pcDNA”. The numbers of CD31-positive blood vessels in the central vs. edge regions of the tumor areas are shown. P values are indicated. Mean ± SD represents three independent experiments. (G–N) Xenograft tumors from “cyclin A1-pcDNA” and “control-pcDNA” groups were immunostained with antibodies against VEGF, VEGFR1, ER-α and Ki67. The representative microphotographs are shown.

    Journal: PLoS ONE

    Article Title: Cyclin A1 Modulates the Expression of Vascular Endothelial Growth Factor and Promotes Hormone-Dependent Growth and Angiogenesis of Breast Cancer

    doi: 10.1371/journal.pone.0072210

    Figure Lengend Snippet: Long-term effect of elevated level of cyclin A1 on growth and angiogenesis phenotype of xenograft tumors in mice. MCF-7 cells stable expressing pcDNA–cyclin A1 or pcDNA vectors were subcutaneous implanted into female nude mice with E2 supplementation. (A, B) Representative microphotographs of xenograft tumor sections stained with Haematoxylin and Eosin are shown. (C, D) Representative pictures show the xenograft tumors stained with antibody against human CD31, the CD31 positive vessels are indicated. The control tumor “control-pcDNA” and cyclin A1 expressing tumors “cyclin A1-pcDNA” are indicated. (E) Growth curves of the two groups of xenograft tumors are indicated. The time is indicated in x-axis and tumor volume in mm 3 is indicated in y-axis. (F) Quantification of the tumor vascularizations in cyclin A1 expressing xenograft tumors “cyclin A1-pcDNA” and in control xenograft tumors “control-pcDNA”. The numbers of CD31-positive blood vessels in the central vs. edge regions of the tumor areas are shown. P values are indicated. Mean ± SD represents three independent experiments. (G–N) Xenograft tumors from “cyclin A1-pcDNA” and “control-pcDNA” groups were immunostained with antibodies against VEGF, VEGFR1, ER-α and Ki67. The representative microphotographs are shown.

    Article Snippet: Polyclonal human anti-CD31 and CDK1 (BD Pharmingen), anti-β-actin, anti-VEGF, anti-VEGF-R1 and VEGF-R2 (Santa Cruz Biotechnology, CA), anti-ER-α, Ki67 (Dako, Golstrup, Denmark) and anti-cyclin D1 (Cell Signaling Technology Inc, Danvers, MA).

    Techniques: Mouse Assay, Expressing, Staining