vascular endothelial growth factor vegf  (Millipore)


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    Name:
    Vascular Endothelial Growth Factor D human
    Description:

    Catalog Number:
    v6012
    Price:
    None
    Applications:
    VEGF-D is involved in the regulation of the growth and/or differentiation of lymphatic endothelium and thus, a mitogen for endothelial cells
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    Structured Review

    Millipore vascular endothelial growth factor vegf
    Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; <t>VEGF.</t> ( F ) <t>IFN</t> gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P

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    Images

    1) Product Images from "Differential response of rat strains to obesogenic diets underlines the importance of genetic makeup of an individual towards obesity"

    Article Title: Differential response of rat strains to obesogenic diets underlines the importance of genetic makeup of an individual towards obesity

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-09149-6

    Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; VEGF. ( F ) IFN gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P
    Figure Legend Snippet: Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; VEGF. ( F ) IFN gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P

    Techniques Used: TBARS Assay

    2) Product Images from "In utero exposure to ultrafine particles promotes placental stress-induced programming of renin-angiotensin system-related elements in the offspring results in altered blood pressure in adult mice"

    Article Title: In utero exposure to ultrafine particles promotes placental stress-induced programming of renin-angiotensin system-related elements in the offspring results in altered blood pressure in adult mice

    Journal: Particle and Fibre Toxicology

    doi: 10.1186/s12989-019-0289-1

    In utero exposure to UFP induces systemic and intrauterine inflammation. Concentrations of pro-inflammatory cytokine: interleukin-β (IL-β), IL-6 and tumor necrosis factor-α (TNF-α). The anti-inflammatory cytokines IL-10 and IL-4. Chemokines: neutrophil chemoattractant (KC), macrophage inflammatory protein 2 (MIP-2) and monocyte chemoattractant protein 1 (MCP-1). Vascular endothelial growth factor (VEGF) ware measured with multiplex magnetic beads in a ) dam serum collected, b ) dam lung, c ) placenta and d ) fetus total protein. n = 4/group. Data are expressed as mean ± SEM. * p
    Figure Legend Snippet: In utero exposure to UFP induces systemic and intrauterine inflammation. Concentrations of pro-inflammatory cytokine: interleukin-β (IL-β), IL-6 and tumor necrosis factor-α (TNF-α). The anti-inflammatory cytokines IL-10 and IL-4. Chemokines: neutrophil chemoattractant (KC), macrophage inflammatory protein 2 (MIP-2) and monocyte chemoattractant protein 1 (MCP-1). Vascular endothelial growth factor (VEGF) ware measured with multiplex magnetic beads in a ) dam serum collected, b ) dam lung, c ) placenta and d ) fetus total protein. n = 4/group. Data are expressed as mean ± SEM. * p

    Techniques Used: In Utero, Multiplex Assay, Magnetic Beads

    3) Product Images from "Insulin-like growth factor I is required for vessel remodeling in the adult brain"

    Article Title: Insulin-like growth factor I is required for vessel remodeling in the adult brain

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.0400337101

    Local IGF-I is required for lesion-induced angiogenesis. ( A ) LID mice receiving a s.c. anti-IGF-I infusion simultaneously to the lesion show a significantly diminished increase in VEGF levels 3 days after the lesion, compared with control injured animals
    Figure Legend Snippet: Local IGF-I is required for lesion-induced angiogenesis. ( A ) LID mice receiving a s.c. anti-IGF-I infusion simultaneously to the lesion show a significantly diminished increase in VEGF levels 3 days after the lesion, compared with control injured animals

    Techniques Used: Mouse Assay

    IGF-I promotes brain angiogenesis. ( A ) IGF-I (10 -7 M) stimulates proliferation of cultured brain endothelial cells as determined with the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide assay. VEGF (10 -7 M) was used as a positive control [
    Figure Legend Snippet: IGF-I promotes brain angiogenesis. ( A ) IGF-I (10 -7 M) stimulates proliferation of cultured brain endothelial cells as determined with the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide assay. VEGF (10 -7 M) was used as a positive control [

    Techniques Used: Cell Culture, Positive Control

    4) Product Images from "A Combretastatin-Mediated Decrease in Neutrophil Concentration in Peripheral Blood and the Impact on the Anti-Tumor Activity of This Drug in Two Different Murine Tumor Models"

    Article Title: A Combretastatin-Mediated Decrease in Neutrophil Concentration in Peripheral Blood and the Impact on the Anti-Tumor Activity of This Drug in Two Different Murine Tumor Models

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0110091

    The effect of CA4P treatment on cytokine levels in C3H mammary carcinomas in CDF1 mice. A) VEGF, B) MIP-1α (CCL3), C) KC (CXCL1), and D) MIP-2 (CXCL2) concentration in tumors as a function of hours after treatment with 25 mg/kg CA4P and in tumors from saline-treated mice. A) and C) Data is presented as mean ± SEM. B) and D) Data is presented as the median (horizontal bar), the 25 th and 75 th percentile (bottom and top of boxes) and the 10 th and 90 th percentiles (error bars). For all data n = 10.
    Figure Legend Snippet: The effect of CA4P treatment on cytokine levels in C3H mammary carcinomas in CDF1 mice. A) VEGF, B) MIP-1α (CCL3), C) KC (CXCL1), and D) MIP-2 (CXCL2) concentration in tumors as a function of hours after treatment with 25 mg/kg CA4P and in tumors from saline-treated mice. A) and C) Data is presented as mean ± SEM. B) and D) Data is presented as the median (horizontal bar), the 25 th and 75 th percentile (bottom and top of boxes) and the 10 th and 90 th percentiles (error bars). For all data n = 10.

    Techniques Used: Mouse Assay, Concentration Assay

    5) Product Images from "In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures"

    Article Title: In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

    Journal: Toxicology Mechanisms and Methods

    doi: 10.3109/15376516.2014.943441

    CYP1A1/1B1 enzyme activity and cytok ine secretion in buccal and gingival tissues. Activity levels of CYP1A1/1B1 were measured at 24 h post-exposure to CS (17.9 and 40.7%) in the buccal (A) and gingival (B) tissues ( N = 3 inserts following a single exposure run). Positive control tests are shown (right side of each panel) using TCDD as an inducer of CYP1A1/1B1 activity. Inflammatory mediators (C) and the corresponding gene expression (D) were measured at 24 h post-exposure of CS compared with the air-exposed tissues ( N = 3 inserts following a single exposure run). Columns are representing different tissues as indicated in the label under the heatmap. Fold changes were obtained by taking the log2 ratio of the cytokine abundance (C) or of the gene expression (D) between the CS group and air-exposed exposure group for every tissue. Welch’s t -test was performed to test the null hypothesis that cytokine abundance (C) or log2-based gene expression (D) in the CS (19.7 and 40.7%) groups and air-exposed group was equivalent. Fold change was set to be zero for the p > 0.05. Blue and red colors indicate negative or positive fold-changes in the CS-exposed tissues as compared to the air-exposed tissues, respectively. Abbreviations: CCL, CC chemokine ligand; CS, cigarette smoke; CYP, cytochrome; G-CSF, granulocyte-colony stimulating factor; GM-CSF, granulocyte macrophage-colony stimulating factor; IL, interleukin; IP-10, interferon gamma inducible protein 10; MCP-1, monocyte chemoattractant protein-1; MMP, matrix metalloproteinase; SEM, standard error of the mean; RANTES, regulated on activation, normal T cell expressed and secreted; RLU, raw luminescence unit; TCDD, 2,3,7,8-tetrachlorodibenzo- p -dioxin; VEGF, vascular endothelial growth factor; PE, post-exposure.
    Figure Legend Snippet: CYP1A1/1B1 enzyme activity and cytok ine secretion in buccal and gingival tissues. Activity levels of CYP1A1/1B1 were measured at 24 h post-exposure to CS (17.9 and 40.7%) in the buccal (A) and gingival (B) tissues ( N = 3 inserts following a single exposure run). Positive control tests are shown (right side of each panel) using TCDD as an inducer of CYP1A1/1B1 activity. Inflammatory mediators (C) and the corresponding gene expression (D) were measured at 24 h post-exposure of CS compared with the air-exposed tissues ( N = 3 inserts following a single exposure run). Columns are representing different tissues as indicated in the label under the heatmap. Fold changes were obtained by taking the log2 ratio of the cytokine abundance (C) or of the gene expression (D) between the CS group and air-exposed exposure group for every tissue. Welch’s t -test was performed to test the null hypothesis that cytokine abundance (C) or log2-based gene expression (D) in the CS (19.7 and 40.7%) groups and air-exposed group was equivalent. Fold change was set to be zero for the p > 0.05. Blue and red colors indicate negative or positive fold-changes in the CS-exposed tissues as compared to the air-exposed tissues, respectively. Abbreviations: CCL, CC chemokine ligand; CS, cigarette smoke; CYP, cytochrome; G-CSF, granulocyte-colony stimulating factor; GM-CSF, granulocyte macrophage-colony stimulating factor; IL, interleukin; IP-10, interferon gamma inducible protein 10; MCP-1, monocyte chemoattractant protein-1; MMP, matrix metalloproteinase; SEM, standard error of the mean; RANTES, regulated on activation, normal T cell expressed and secreted; RLU, raw luminescence unit; TCDD, 2,3,7,8-tetrachlorodibenzo- p -dioxin; VEGF, vascular endothelial growth factor; PE, post-exposure.

    Techniques Used: Activity Assay, Positive Control, Expressing, Activation Assay

    6) Product Images from "Macrophages activate mesenchymal stem cells to acquire cancer-associated fibroblast-like features resulting in gastric epithelial cell lesions and malignant transformation in vitro"

    Article Title: Macrophages activate mesenchymal stem cells to acquire cancer-associated fibroblast-like features resulting in gastric epithelial cell lesions and malignant transformation in vitro

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.9703

    High levels of IL-8, PDGF-B, GM-CSF, IL-6, MCP-1, VEGF and TNF were presented in the supernatant from macrophage-hucMSCs. (A) The expression of cytokines (IL-8, P=0.0092; PDGF-B, P=0.0391; GM-CSF, P=0.0095; IL-6, P=0.0077; MCP-1, P=0.0084; VEGF, P=0.0441; TNF, P=0.0244) in the supernatant of the hucMSCs cultured with or without macrophages were measured using a Luminex assay. (B) mRNA expressions of IL-8, PDGF-B, GM-CSF, IL-6, MCP-1 and TNF significantly increased in the macrophage-hucMSCs. (C) Supernatants from the two aforementioned types of cells were assessed for IL-8, GM-CSF, IL-6 and MCP-1 expression by ELISA. Data are presented as the mean ± standard deviation; *P
    Figure Legend Snippet: High levels of IL-8, PDGF-B, GM-CSF, IL-6, MCP-1, VEGF and TNF were presented in the supernatant from macrophage-hucMSCs. (A) The expression of cytokines (IL-8, P=0.0092; PDGF-B, P=0.0391; GM-CSF, P=0.0095; IL-6, P=0.0077; MCP-1, P=0.0084; VEGF, P=0.0441; TNF, P=0.0244) in the supernatant of the hucMSCs cultured with or without macrophages were measured using a Luminex assay. (B) mRNA expressions of IL-8, PDGF-B, GM-CSF, IL-6, MCP-1 and TNF significantly increased in the macrophage-hucMSCs. (C) Supernatants from the two aforementioned types of cells were assessed for IL-8, GM-CSF, IL-6 and MCP-1 expression by ELISA. Data are presented as the mean ± standard deviation; *P

    Techniques Used: Expressing, Cell Culture, Luminex, Enzyme-linked Immunosorbent Assay, Standard Deviation

    7) Product Images from "Monocarboxylate transporter 1 (MCT1), a tool to stratify acute myeloid leukemia (AML) patients and a vehicle to kill cancer cells"

    Article Title: Monocarboxylate transporter 1 (MCT1), a tool to stratify acute myeloid leukemia (AML) patients and a vehicle to kill cancer cells

    Journal: Oncotarget

    doi: 10.18632/oncotarget.20294

    Expression of MCT1, MCT4 and LDH isoenzymes under lactate and VEGF stimuli Immunofluorescense and western blotting was performed in order to evaluate the effect of lactate and VEGF in the expression of MCT1 and MCT4, in HL60, THP1 and HEL cell lines. Immunofluorescense for the detection of MCT1 (A) and MCT4 (B) , western bloting for MCT1 and MCT4 (C) which were respectively quantified (D and E) using control conditions of each cell line after normalization for β-actin and (F) evaluation of LDH isoenzymes in an agarose gel electrophoresis (LDH Isoenzymes Electrophoresis Kit; SRE612K, Interlab) and bands quantification in an EasyFix Interlab G26 equipment. C-Control, L-Lactate, V-VEGF, LV-NaLac plus VEGF. Error bars represent standard deviation; statistical significance **p
    Figure Legend Snippet: Expression of MCT1, MCT4 and LDH isoenzymes under lactate and VEGF stimuli Immunofluorescense and western blotting was performed in order to evaluate the effect of lactate and VEGF in the expression of MCT1 and MCT4, in HL60, THP1 and HEL cell lines. Immunofluorescense for the detection of MCT1 (A) and MCT4 (B) , western bloting for MCT1 and MCT4 (C) which were respectively quantified (D and E) using control conditions of each cell line after normalization for β-actin and (F) evaluation of LDH isoenzymes in an agarose gel electrophoresis (LDH Isoenzymes Electrophoresis Kit; SRE612K, Interlab) and bands quantification in an EasyFix Interlab G26 equipment. C-Control, L-Lactate, V-VEGF, LV-NaLac plus VEGF. Error bars represent standard deviation; statistical significance **p

    Techniques Used: Expressing, Western Blot, Agarose Gel Electrophoresis, Electrophoresis, Standard Deviation

    8) Product Images from "Increased toll-like receptors and p53 levels regulate apoptosis and angiogenesis in non-muscle invasive bladder cancer: mechanism of action of P-MAPA biological response modifier"

    Article Title: Increased toll-like receptors and p53 levels regulate apoptosis and angiogenesis in non-muscle invasive bladder cancer: mechanism of action of P-MAPA biological response modifier

    Journal: BMC Cancer

    doi: 10.1186/s12885-016-2474-z

    Representative Western Blotting and semiquantitative determination for VEGF, Endostatin, BAX and NLRC5 protein levels. Samples of urinary bladder were pooled from five animals per group for each repetition (duplicate) and used for semi-quantitative densitometry (IOD – Integrated Optical Density) analysis of the VEGF, Endostatin, BAX and NLRC5 levels following normalization to the β-actin. All data were expressed as the mean ± standard deviation. Different lowercase letters ( a, b, c, d ) indicate significant differences ( p
    Figure Legend Snippet: Representative Western Blotting and semiquantitative determination for VEGF, Endostatin, BAX and NLRC5 protein levels. Samples of urinary bladder were pooled from five animals per group for each repetition (duplicate) and used for semi-quantitative densitometry (IOD – Integrated Optical Density) analysis of the VEGF, Endostatin, BAX and NLRC5 levels following normalization to the β-actin. All data were expressed as the mean ± standard deviation. Different lowercase letters ( a, b, c, d ) indicate significant differences ( p

    Techniques Used: Western Blot, Standard Deviation

    9) Product Images from "Bioengineering Vascular Networks to Study Angiogenesis and Vascularization of Physiologically Relevant Tissue Models in Vitro"

    Article Title: Bioengineering Vascular Networks to Study Angiogenesis and Vascularization of Physiologically Relevant Tissue Models in Vitro

    Journal: ACS Biomaterials Science & Engineering

    doi: 10.1021/acsbiomaterials.0c00191

    Outgrowing HDMECs from PHBV channels to either plain or VEGF or 2dDR loaded Matrigel. The ECs were stained with phalloidin-TRITC (red) to visualize actin filaments and counterstained with DAPI (blue) to visualize the cell nuclei. Tubelike formed structures were obvious and well-organized in VEGF loaded Matrigel groups when compared with 2dDR loaded and control groups. The graphs show the increase in the number of tubes formed (on the left) and branch points (on the right) within Matrigel when VEGF and 2dDR were loaded (*** p ≤ 0.001, * p ≤ 0.05).
    Figure Legend Snippet: Outgrowing HDMECs from PHBV channels to either plain or VEGF or 2dDR loaded Matrigel. The ECs were stained with phalloidin-TRITC (red) to visualize actin filaments and counterstained with DAPI (blue) to visualize the cell nuclei. Tubelike formed structures were obvious and well-organized in VEGF loaded Matrigel groups when compared with 2dDR loaded and control groups. The graphs show the increase in the number of tubes formed (on the left) and branch points (on the right) within Matrigel when VEGF and 2dDR were loaded (*** p ≤ 0.001, * p ≤ 0.05).

    Techniques Used: Staining

    10) Product Images from "Ontogeny of the Kidney and Renal Developmental Markers in the Rhesus Monkey (Macaca mulatta)"

    Article Title: Ontogeny of the Kidney and Renal Developmental Markers in the Rhesus Monkey (Macaca mulatta)

    Journal: Anatomical record (Hoboken, N.J. : 2007)

    doi: 10.1002/ar.21242

    Markers of glomerular tuft development including CD31, Gremlin, alpha-smooth muscle actin (α-SMA), and vascular endothelial growth factor (VEGF) in sequential stages of nephrogenesis in rhesus monkeys
    Figure Legend Snippet: Markers of glomerular tuft development including CD31, Gremlin, alpha-smooth muscle actin (α-SMA), and vascular endothelial growth factor (VEGF) in sequential stages of nephrogenesis in rhesus monkeys

    Techniques Used:

    11) Product Images from "Anti-Angiogenic Activity of Flunarizine by In Ovo, In Vitro, and In Vivo Assays"

    Article Title: Anti-Angiogenic Activity of Flunarizine by In Ovo, In Vitro, and In Vivo Assays

    Journal: Turkish Journal of Pharmaceutical Sciences

    doi: 10.4274/tjps.galenos.2018.29981

    Modulation of endothelial cell responses to FLN, Bevacizumab and VEGF. (a) Cell proliferation was determined by cell counting with a hemocytometer. (b) Representative images of tube formation after being treated with FLN for 2 h following VEGF stimulation. (c) Quantitative data of scratch wound-healing inhibition in HUVECs treated with FLN for 24 h under VEGF stimulation. Cord-like network morphogenesis in vitro is affected by K ATP modulation FLN: Flunarizine, VEGF: Vascular endothelial growth factor, HUVECs: Human umbilical vein endothelial cells
    Figure Legend Snippet: Modulation of endothelial cell responses to FLN, Bevacizumab and VEGF. (a) Cell proliferation was determined by cell counting with a hemocytometer. (b) Representative images of tube formation after being treated with FLN for 2 h following VEGF stimulation. (c) Quantitative data of scratch wound-healing inhibition in HUVECs treated with FLN for 24 h under VEGF stimulation. Cord-like network morphogenesis in vitro is affected by K ATP modulation FLN: Flunarizine, VEGF: Vascular endothelial growth factor, HUVECs: Human umbilical vein endothelial cells

    Techniques Used: Cell Counting, Inhibition, In Vitro

    12) Product Images from "Regorafenib inhibits tumor progression through suppression of ERK/NF-κB activation in hepatocellular carcinoma bearing mice"

    Article Title: Regorafenib inhibits tumor progression through suppression of ERK/NF-κB activation in hepatocellular carcinoma bearing mice

    Journal: Bioscience Reports

    doi: 10.1042/BSR20171264

    Effect of regorafenib on expression of P-ERK, AKT (Ser473), NF-κB p65 (Ser473), and NF-κB-modulated downstream effector proteins in SK-Hep1/ luc2 tumor and Hep3B 2.1-7 bearing mice Mice were killed on day 14 after treatments and protein levels in tumor tissues were evaluated with IHC staining. ( A ) Protein levels of MMP-9, VEGF, MCL-1, XIAP, C-FLIP, Cyclin-D1, and P-ERK, AKT (Ser473), NF-κB p65 (Ser473) on SK-Hep1/ luc2 tumor by IHC. ( B ) IHC staining of Hep3B 2.1-7 tumor. ( C ) Phosphorylation oncogenes and apoptosis-related cleavage proteins expression which validated by Western blotting on SK-Hep1/ luc2 tumor. ( D ) Western blotting of Hep3B 2.1-7 tumor. ( E ) Expression of antiapoptotic proteins (active Capase-9, -8, and -3) on SK-Hep1/ luc2 tumor by IHC. ( F ) IHC staining of Hep3B 2.1-7 tumor; a P
    Figure Legend Snippet: Effect of regorafenib on expression of P-ERK, AKT (Ser473), NF-κB p65 (Ser473), and NF-κB-modulated downstream effector proteins in SK-Hep1/ luc2 tumor and Hep3B 2.1-7 bearing mice Mice were killed on day 14 after treatments and protein levels in tumor tissues were evaluated with IHC staining. ( A ) Protein levels of MMP-9, VEGF, MCL-1, XIAP, C-FLIP, Cyclin-D1, and P-ERK, AKT (Ser473), NF-κB p65 (Ser473) on SK-Hep1/ luc2 tumor by IHC. ( B ) IHC staining of Hep3B 2.1-7 tumor. ( C ) Phosphorylation oncogenes and apoptosis-related cleavage proteins expression which validated by Western blotting on SK-Hep1/ luc2 tumor. ( D ) Western blotting of Hep3B 2.1-7 tumor. ( E ) Expression of antiapoptotic proteins (active Capase-9, -8, and -3) on SK-Hep1/ luc2 tumor by IHC. ( F ) IHC staining of Hep3B 2.1-7 tumor; a P

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining, Western Blot

    13) Product Images from "Regorafenib inhibits tumor progression through suppression of ERK/NF-κB activation in hepatocellular carcinoma bearing mice"

    Article Title: Regorafenib inhibits tumor progression through suppression of ERK/NF-κB activation in hepatocellular carcinoma bearing mice

    Journal: Bioscience Reports

    doi: 10.1042/BSR20171264

    Effect of regorafenib on expression of P-ERK, AKT (Ser473), NF-κB p65 (Ser473), and NF-κB-modulated downstream effector proteins in SK-Hep1/ luc2 tumor and Hep3B 2.1-7 bearing mice Mice were killed on day 14 after treatments and protein levels in tumor tissues were evaluated with IHC staining. ( A ) Protein levels of MMP-9, VEGF, MCL-1, XIAP, C-FLIP, Cyclin-D1, and P-ERK, AKT (Ser473), NF-κB p65 (Ser473) on SK-Hep1/ luc2 tumor by IHC. ( B ) IHC staining of Hep3B 2.1-7 tumor. ( C ) Phosphorylation oncogenes and apoptosis-related cleavage proteins expression which validated by Western blotting on SK-Hep1/ luc2 tumor. ( D ) Western blotting of Hep3B 2.1-7 tumor. ( E ) Expression of antiapoptotic proteins (active Capase-9, -8, and -3) on SK-Hep1/ luc2 tumor by IHC. ( F ) IHC staining of Hep3B 2.1-7 tumor; a P
    Figure Legend Snippet: Effect of regorafenib on expression of P-ERK, AKT (Ser473), NF-κB p65 (Ser473), and NF-κB-modulated downstream effector proteins in SK-Hep1/ luc2 tumor and Hep3B 2.1-7 bearing mice Mice were killed on day 14 after treatments and protein levels in tumor tissues were evaluated with IHC staining. ( A ) Protein levels of MMP-9, VEGF, MCL-1, XIAP, C-FLIP, Cyclin-D1, and P-ERK, AKT (Ser473), NF-κB p65 (Ser473) on SK-Hep1/ luc2 tumor by IHC. ( B ) IHC staining of Hep3B 2.1-7 tumor. ( C ) Phosphorylation oncogenes and apoptosis-related cleavage proteins expression which validated by Western blotting on SK-Hep1/ luc2 tumor. ( D ) Western blotting of Hep3B 2.1-7 tumor. ( E ) Expression of antiapoptotic proteins (active Capase-9, -8, and -3) on SK-Hep1/ luc2 tumor by IHC. ( F ) IHC staining of Hep3B 2.1-7 tumor; a P

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining, Western Blot

    14) Product Images from "Normal ageing is associated with an increase in Th2 cells, MCP-1 (CCL1) and RANTES (CCL5), with differences in sCD40L and PDGF-AA between sexes"

    Article Title: Normal ageing is associated with an increase in Th2 cells, MCP-1 (CCL1) and RANTES (CCL5), with differences in sCD40L and PDGF-AA between sexes

    Journal: Clinical and Experimental Immunology

    doi: 10.1111/j.1365-2249.2012.04644.x

    Principal components analysis (PCA) of cytokines between sexes by age. PCA identified growth-regulated oncogene (GRO) (CXCL1), platelet-derived growth factor (PDGF)-AA, sCD40L, interleukin (IL)-8, vascular endothelial growth factor (VEGF) and fibroblast
    Figure Legend Snippet: Principal components analysis (PCA) of cytokines between sexes by age. PCA identified growth-regulated oncogene (GRO) (CXCL1), platelet-derived growth factor (PDGF)-AA, sCD40L, interleukin (IL)-8, vascular endothelial growth factor (VEGF) and fibroblast

    Techniques Used: Derivative Assay

    15) Product Images from "Amentoflavone Inhibits ERK-modulated Tumor Progression in Hepatocellular Carcinoma In Vitro"

    Article Title: Amentoflavone Inhibits ERK-modulated Tumor Progression in Hepatocellular Carcinoma In Vitro

    Journal: In Vivo

    doi: 10.21873/invivo.112274

    3. Effect of PD98059 and amentoflavone on the expression of tumor progression-associated proteins in SK-Hep1 cells. Cells were treated with 10 μM PD98059, or different concentrations (0, 100, 200 μM) of amentoflavone for 48 h, respectively. Protein levels of MMP-9, XIAP, VEGF, cyclin-D1, and pERK were evaluated with western blotting assay. (A) PD98059 treatment, (B) Amentoflavone treatment.
    Figure Legend Snippet: 3. Effect of PD98059 and amentoflavone on the expression of tumor progression-associated proteins in SK-Hep1 cells. Cells were treated with 10 μM PD98059, or different concentrations (0, 100, 200 μM) of amentoflavone for 48 h, respectively. Protein levels of MMP-9, XIAP, VEGF, cyclin-D1, and pERK were evaluated with western blotting assay. (A) PD98059 treatment, (B) Amentoflavone treatment.

    Techniques Used: Expressing, Western Blot

    16) Product Images from "Cellular prion protein and γ-synuclein overexpression in LS 174T colorectal cancer cell drives endothelial proliferation-to-differentiation switch"

    Article Title: Cellular prion protein and γ-synuclein overexpression in LS 174T colorectal cancer cell drives endothelial proliferation-to-differentiation switch

    Journal: PeerJ

    doi: 10.7717/peerj.4506

    Overexpression of γ-Syn and PrPC inhibits LS 174T cells from adhering onto EA. (A) Cell attachment analysis of the adhesiveness of unstimulated and stimulated (VEGF, 20 ng/mL) LS 174T cell lines on EA. Images were taken at 100× magnification using the Eclipse TS100 inverted microscope (Nikon, New York, NY, USA). (B) Fluorescence intensity was quantified. Data were expressed as fluorescence intensity and represent the mean ± SEM (error bars) of three independent experiments. Mean values were compared using one-way ANOVA followed by LSD’s post hoc test. Asterisk indicates p
    Figure Legend Snippet: Overexpression of γ-Syn and PrPC inhibits LS 174T cells from adhering onto EA. (A) Cell attachment analysis of the adhesiveness of unstimulated and stimulated (VEGF, 20 ng/mL) LS 174T cell lines on EA. Images were taken at 100× magnification using the Eclipse TS100 inverted microscope (Nikon, New York, NY, USA). (B) Fluorescence intensity was quantified. Data were expressed as fluorescence intensity and represent the mean ± SEM (error bars) of three independent experiments. Mean values were compared using one-way ANOVA followed by LSD’s post hoc test. Asterisk indicates p

    Techniques Used: Over Expression, Cell Attachment Assay, Inverted Microscopy, Fluorescence

    Partial secretome analysis of conditioned media from LS 174T cell lines. (A) Angiogenesis antibody array proteome profiling of CM of LS 174T cell lines. Red boxes indicate reference spots whereas green boxes indicate negative control. Numbered yellow boxes indicate the ten angiogenic factors in the CM that yielded distinct signals, which are CXCL16 (1), DPPIV (2), TIMP-1 (3), TIMP-4 (4), angiogenin (5), IGFBP-2 (6), uPA (7), IL-8 (8), amphiregulin (9) and VEGF (10). (B) Secretion levels of different angiogenesis-related proteins in LS 174T CM, LS 174T-γ-Syn CM and LS 174T-PrP CM were analyzed with ImageJ software and presented as densitometry values. (C) The ten angiogenic factors that yielded distinct signals were relatively quantified. Data of densitometry value were expressed as relative intensity compared to LS 174T CM (set as 1) and represent the mean ± SEM (error bars) of two independent experiments. Mean values were compared using one-way ANOVA followed by LSD’s post hoc test. Asterisk indicates p
    Figure Legend Snippet: Partial secretome analysis of conditioned media from LS 174T cell lines. (A) Angiogenesis antibody array proteome profiling of CM of LS 174T cell lines. Red boxes indicate reference spots whereas green boxes indicate negative control. Numbered yellow boxes indicate the ten angiogenic factors in the CM that yielded distinct signals, which are CXCL16 (1), DPPIV (2), TIMP-1 (3), TIMP-4 (4), angiogenin (5), IGFBP-2 (6), uPA (7), IL-8 (8), amphiregulin (9) and VEGF (10). (B) Secretion levels of different angiogenesis-related proteins in LS 174T CM, LS 174T-γ-Syn CM and LS 174T-PrP CM were analyzed with ImageJ software and presented as densitometry values. (C) The ten angiogenic factors that yielded distinct signals were relatively quantified. Data of densitometry value were expressed as relative intensity compared to LS 174T CM (set as 1) and represent the mean ± SEM (error bars) of two independent experiments. Mean values were compared using one-way ANOVA followed by LSD’s post hoc test. Asterisk indicates p

    Techniques Used: Ab Array, Negative Control, Software

    17) Product Images from "Candidate Markers Associated with the Probability of Future Pulmonary Exacerbations in Cystic Fibrosis Patients"

    Article Title: Candidate Markers Associated with the Probability of Future Pulmonary Exacerbations in Cystic Fibrosis Patients

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0088567

    Percentage change in inflammatory markers throughout PEs in CF. Inflammatory markers were measured in blood plasma from CF patients throughout PE. The percentage change was calculated at each time point and compared to a 0% change which indicates no change from baseline (represented by dotted horizontal line). A) CRP levels were significantly increased from baseline values on Day 1 of PE (p = 0.001) and Day 42 (p = 0.039). B) IL-6 levels were significantly higher on Day 1 of PE (p = 0.006). C) IL-8 concentrations were significantly higher on Day 1 (p = 0.047) and significantly lower than baseline values after treatment on Day 21 (p = 0.022). D) IL-10 levels rose significantly on Days 7 (p = 0.021) and Day 14 (p = 0.046). E) MIP-1β increased from baseline on Days 1 (p = 0.020) and Day 42 (p = 0.023). F) VEGF levels were significantly higher on Day 1 of PE (p = 0.043). Solid horizontal lines are set at the mean * indicates a significant difference from 0% change from baseline. Day 1, n = 13; Day 7, n = 12, Day 14, n = 11; Day 21, n = 9; Day 42, n = 8. Full data set is found in Table S2 .
    Figure Legend Snippet: Percentage change in inflammatory markers throughout PEs in CF. Inflammatory markers were measured in blood plasma from CF patients throughout PE. The percentage change was calculated at each time point and compared to a 0% change which indicates no change from baseline (represented by dotted horizontal line). A) CRP levels were significantly increased from baseline values on Day 1 of PE (p = 0.001) and Day 42 (p = 0.039). B) IL-6 levels were significantly higher on Day 1 of PE (p = 0.006). C) IL-8 concentrations were significantly higher on Day 1 (p = 0.047) and significantly lower than baseline values after treatment on Day 21 (p = 0.022). D) IL-10 levels rose significantly on Days 7 (p = 0.021) and Day 14 (p = 0.046). E) MIP-1β increased from baseline on Days 1 (p = 0.020) and Day 42 (p = 0.023). F) VEGF levels were significantly higher on Day 1 of PE (p = 0.043). Solid horizontal lines are set at the mean * indicates a significant difference from 0% change from baseline. Day 1, n = 13; Day 7, n = 12, Day 14, n = 11; Day 21, n = 9; Day 42, n = 8. Full data set is found in Table S2 .

    Techniques Used:

    18) Product Images from "Cellular prion protein and γ-synuclein overexpression in LS 174T colorectal cancer cell drives endothelial proliferation-to-differentiation switch"

    Article Title: Cellular prion protein and γ-synuclein overexpression in LS 174T colorectal cancer cell drives endothelial proliferation-to-differentiation switch

    Journal: PeerJ

    doi: 10.7717/peerj.4506

    Overexpression of γ-Syn and PrPC inhibits LS 174T cells from adhering onto EA. (A) Cell attachment analysis of the adhesiveness of unstimulated and stimulated (VEGF, 20 ng/mL) LS 174T cell lines on EA. Images were taken at 100× magnification using the Eclipse TS100 inverted microscope (Nikon, New York, NY, USA). (B) Fluorescence intensity was quantified. Data were expressed as fluorescence intensity and represent the mean ± SEM (error bars) of three independent experiments. Mean values were compared using one-way ANOVA followed by LSD’s post hoc test. Asterisk indicates p
    Figure Legend Snippet: Overexpression of γ-Syn and PrPC inhibits LS 174T cells from adhering onto EA. (A) Cell attachment analysis of the adhesiveness of unstimulated and stimulated (VEGF, 20 ng/mL) LS 174T cell lines on EA. Images were taken at 100× magnification using the Eclipse TS100 inverted microscope (Nikon, New York, NY, USA). (B) Fluorescence intensity was quantified. Data were expressed as fluorescence intensity and represent the mean ± SEM (error bars) of three independent experiments. Mean values were compared using one-way ANOVA followed by LSD’s post hoc test. Asterisk indicates p

    Techniques Used: Over Expression, Cell Attachment Assay, Inverted Microscopy, Fluorescence

    Partial secretome analysis of conditioned media from LS 174T cell lines. (A) Angiogenesis antibody array proteome profiling of CM of LS 174T cell lines. Red boxes indicate reference spots whereas green boxes indicate negative control. Numbered yellow boxes indicate the ten angiogenic factors in the CM that yielded distinct signals, which are CXCL16 (1), DPPIV (2), TIMP-1 (3), TIMP-4 (4), angiogenin (5), IGFBP-2 (6), uPA (7), IL-8 (8), amphiregulin (9) and VEGF (10). (B) Secretion levels of different angiogenesis-related proteins in LS 174T CM, LS 174T-γ-Syn CM and LS 174T-PrP CM were analyzed with ImageJ software and presented as densitometry values. (C) The ten angiogenic factors that yielded distinct signals were relatively quantified. Data of densitometry value were expressed as relative intensity compared to LS 174T CM (set as 1) and represent the mean ± SEM (error bars) of two independent experiments. Mean values were compared using one-way ANOVA followed by LSD’s post hoc test. Asterisk indicates p
    Figure Legend Snippet: Partial secretome analysis of conditioned media from LS 174T cell lines. (A) Angiogenesis antibody array proteome profiling of CM of LS 174T cell lines. Red boxes indicate reference spots whereas green boxes indicate negative control. Numbered yellow boxes indicate the ten angiogenic factors in the CM that yielded distinct signals, which are CXCL16 (1), DPPIV (2), TIMP-1 (3), TIMP-4 (4), angiogenin (5), IGFBP-2 (6), uPA (7), IL-8 (8), amphiregulin (9) and VEGF (10). (B) Secretion levels of different angiogenesis-related proteins in LS 174T CM, LS 174T-γ-Syn CM and LS 174T-PrP CM were analyzed with ImageJ software and presented as densitometry values. (C) The ten angiogenic factors that yielded distinct signals were relatively quantified. Data of densitometry value were expressed as relative intensity compared to LS 174T CM (set as 1) and represent the mean ± SEM (error bars) of two independent experiments. Mean values were compared using one-way ANOVA followed by LSD’s post hoc test. Asterisk indicates p

    Techniques Used: Ab Array, Negative Control, Software

    19) Product Images from "Mouse population-based evaluation of urinary protein and miRNA biomarker performance associated with cisplatin renal injury"

    Article Title: Mouse population-based evaluation of urinary protein and miRNA biomarker performance associated with cisplatin renal injury

    Journal: Experimental Biology and Medicine

    doi: 10.1177/1535370217740854

    BUN and urinary protein biomarkers separated by necrosis score. (a) Concentrations of BUN are shown for DO mice and are separated by severity score for renal tubule necrosis. Similarly, abundance of urinary proteins normalized to urine volume collected over 18 h is shown in (b–l). Analytes shown include (b) B2M, (c) renin, (d) KIM-1, (e) TIMP-1, (f) IP-10, (g) VEGF, (h) cystatin C, (i) NGAL, (j) EGF, (k) clusterin, and (l) OPN. Bars indicate ± SD. a: p
    Figure Legend Snippet: BUN and urinary protein biomarkers separated by necrosis score. (a) Concentrations of BUN are shown for DO mice and are separated by severity score for renal tubule necrosis. Similarly, abundance of urinary proteins normalized to urine volume collected over 18 h is shown in (b–l). Analytes shown include (b) B2M, (c) renin, (d) KIM-1, (e) TIMP-1, (f) IP-10, (g) VEGF, (h) cystatin C, (i) NGAL, (j) EGF, (k) clusterin, and (l) OPN. Bars indicate ± SD. a: p

    Techniques Used: Mouse Assay

    20) Product Images from "Liposome encapsulated zoledronate favours M1-like behaviour in murine macrophages cultured with soluble factors from breast cancer cells"

    Article Title: Liposome encapsulated zoledronate favours M1-like behaviour in murine macrophages cultured with soluble factors from breast cancer cells

    Journal: BMC Cancer

    doi: 10.1186/s12885-015-1005-7

    Effect of BPs on the response to LPS in 4T1CM-treated J774 cells. A mRNA expression of iNOS, IL-6, IL-12, and MMP-9. B Protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6 secreted by macrophages. *p
    Figure Legend Snippet: Effect of BPs on the response to LPS in 4T1CM-treated J774 cells. A mRNA expression of iNOS, IL-6, IL-12, and MMP-9. B Protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6 secreted by macrophages. *p

    Techniques Used: Expressing

    Different mRNA and secreted cytokine profiles of 4T1 and 3T3 cells lead to differences in the modulation of J774 cell activation status with or without LPS stimulus. A mRNA expression levels of iNOS, IL-6, IL-12, and MMP-9. B Protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6 secreted by macrophages. C Nitrite (NO 2 - ) production. Data represent the means ± SEM of 5 independent experiments. *p
    Figure Legend Snippet: Different mRNA and secreted cytokine profiles of 4T1 and 3T3 cells lead to differences in the modulation of J774 cell activation status with or without LPS stimulus. A mRNA expression levels of iNOS, IL-6, IL-12, and MMP-9. B Protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6 secreted by macrophages. C Nitrite (NO 2 - ) production. Data represent the means ± SEM of 5 independent experiments. *p

    Techniques Used: Activation Assay, Expressing

    21) Product Images from "In utero exposure to ultrafine particles promotes placental stress-induced programming of renin-angiotensin system-related elements in the offspring results in altered blood pressure in adult mice"

    Article Title: In utero exposure to ultrafine particles promotes placental stress-induced programming of renin-angiotensin system-related elements in the offspring results in altered blood pressure in adult mice

    Journal: Particle and Fibre Toxicology

    doi: 10.1186/s12989-019-0289-1

    In utero exposure to UFP induces systemic and intrauterine inflammation. Concentrations of pro-inflammatory cytokine: interleukin-β (IL-β), IL-6 and tumor necrosis factor-α (TNF-α). The anti-inflammatory cytokines IL-10 and IL-4. Chemokines: neutrophil chemoattractant (KC), macrophage inflammatory protein 2 (MIP-2) and monocyte chemoattractant protein 1 (MCP-1). Vascular endothelial growth factor (VEGF) ware measured with multiplex magnetic beads in a ) dam serum collected, b ) dam lung, c ) placenta and d ) fetus total protein. n = 4/group. Data are expressed as mean ± SEM. * p
    Figure Legend Snippet: In utero exposure to UFP induces systemic and intrauterine inflammation. Concentrations of pro-inflammatory cytokine: interleukin-β (IL-β), IL-6 and tumor necrosis factor-α (TNF-α). The anti-inflammatory cytokines IL-10 and IL-4. Chemokines: neutrophil chemoattractant (KC), macrophage inflammatory protein 2 (MIP-2) and monocyte chemoattractant protein 1 (MCP-1). Vascular endothelial growth factor (VEGF) ware measured with multiplex magnetic beads in a ) dam serum collected, b ) dam lung, c ) placenta and d ) fetus total protein. n = 4/group. Data are expressed as mean ± SEM. * p

    Techniques Used: In Utero, Multiplex Assay, Magnetic Beads

    22) Product Images from "Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis"

    Article Title: Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2015.3409

    A model of the paracrine mechanisms of UC-MSCs in tissue repair. In damaged tissues, UC-MSCs attract stem/progenitor cells via paracrine activity involving SDF-1/CXCR4 and MCP-1/CCR2 interaction. Potent paracrine chemoattractant and angiogenic factors affect the microenvironment by acting on different cell types, leading to tissue repair and angiogenesis. UC-MSCs, umbilical cord mesenchymal stem cells; SDF-1, stromal cell-derived factor 1; CXCR4, C-X-C chemokine receptor 4; c-met, MCP-1, monocyte chemotactic protein 1; HGF, hepatocyte growth factor; CCR2, C-C chemokine receptor 2; HUVECs, human umbilical vein endothelial cells; IGF, insulin-like growth factor; VEGF, vascular endothelial growth factor; IL, interleukin; VCAM, vascular cell adhesion protein.
    Figure Legend Snippet: A model of the paracrine mechanisms of UC-MSCs in tissue repair. In damaged tissues, UC-MSCs attract stem/progenitor cells via paracrine activity involving SDF-1/CXCR4 and MCP-1/CCR2 interaction. Potent paracrine chemoattractant and angiogenic factors affect the microenvironment by acting on different cell types, leading to tissue repair and angiogenesis. UC-MSCs, umbilical cord mesenchymal stem cells; SDF-1, stromal cell-derived factor 1; CXCR4, C-X-C chemokine receptor 4; c-met, MCP-1, monocyte chemotactic protein 1; HGF, hepatocyte growth factor; CCR2, C-C chemokine receptor 2; HUVECs, human umbilical vein endothelial cells; IGF, insulin-like growth factor; VEGF, vascular endothelial growth factor; IL, interleukin; VCAM, vascular cell adhesion protein.

    Techniques Used: Activity Assay, Derivative Assay

    23) Product Images from "CCL2 Overexpression in the Brain Promotes Glial Activation and Accelerates Tau Pathology in a Mouse Model of Tauopathy"

    Article Title: CCL2 Overexpression in the Brain Promotes Glial Activation and Accelerates Tau Pathology in a Mouse Model of Tauopathy

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.00997

    Cytokine levels following expression of CCL2 in rTg4510 mice. The concentrations of Interleukin 1 alpha (IL-1α), Interleukin 1 beta (IL-1β), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Interleukin 13 (IL-13), C-C motif ligand 11 (CCL11), Keratinocyte chemoattractant (KC), C-C motif ligand 3 (CCL3), C-C motif ligand 5 (CCL5), Vascular endothelial growth factor (VEGF) and Tumor necrosis factor alpha (TNF-α) were measured using the mouse cytokine/chemokine panel (MILLIPLEX MAP kit; Millipore, Billerica, MA, USA) in CCL2 injected mice and RFP controls. (mean ± S.E.M, n = 6). Statistical analysis was performed using multiple t -test analysis (* p
    Figure Legend Snippet: Cytokine levels following expression of CCL2 in rTg4510 mice. The concentrations of Interleukin 1 alpha (IL-1α), Interleukin 1 beta (IL-1β), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Interleukin 13 (IL-13), C-C motif ligand 11 (CCL11), Keratinocyte chemoattractant (KC), C-C motif ligand 3 (CCL3), C-C motif ligand 5 (CCL5), Vascular endothelial growth factor (VEGF) and Tumor necrosis factor alpha (TNF-α) were measured using the mouse cytokine/chemokine panel (MILLIPLEX MAP kit; Millipore, Billerica, MA, USA) in CCL2 injected mice and RFP controls. (mean ± S.E.M, n = 6). Statistical analysis was performed using multiple t -test analysis (* p

    Techniques Used: Expressing, Mouse Assay, Injection

    24) Product Images from "BCG Vaccination Induces Different Cytokine Profiles Following Infant BCG Vaccination in the UK and Malawi"

    Article Title: BCG Vaccination Induces Different Cytokine Profiles Following Infant BCG Vaccination in the UK and Malawi

    Journal: The Journal of Infectious Diseases

    doi: 10.1093/infdis/jir515

    Cytokine responses measured by 42-multiplex assay. A, Proinflammatory cytokines; B, T helper 2, regulatory, and T cell activation cytokines; C, Chemokines; and D, Growth factors in supernatants from diluted whole-blood cultures that were stimulated with Mycobacterium tuberculosis purified protein derivative for 6 days were measured using a 42-multiplex assay 3 months and 12 months after BCG vaccination in UK and Malawian infants (3 months: UK n = 28, Malawi n = 40; 12 months: UK n = 26, Malawi n = 36). Line represents median response. EGF, epidermal growth factor; FGF-2, fibroblast growth factor 2; Flt3-L, FMS-like tyrosine kinase 3 ligand; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte macrophage colony-stimulating factor; GRO, growth regulated oncogene; IFN-α, interferon α; IFN-γ, interferon-γ; IL-1α, interleukin 1α; IL-1β, interleukin 1β; IL-1RA, interleukin 1receptor antagonist; IL-2, interleukin 2; IL-3, interleukin 3; IL-4, interleukin 4; IL-5, interleukin 5; IL-6, interleukin 6; IL-7, interleukin 7; IL-9, interleukin 9; IL-10, interleukin 10; IL-12p40, interleukin 12p40; IL-12p70, interleukin 12p70; IL-13, interleukin 13; IL-17, interleukin 17; IP-10 (IFN-γ inducible protein 10); MCP-3, monocyte chemotactic protein 3; MDC, macrophage-derived chemokine; MIP-1α, macrophage inflammatory protein 1α; MIP-1β, macrophage inflammatory protein 1β; PDGF-AA, platelet derived growth factor AA; PDGF-AB/BB, platelet derived growth factor AB/BB; RANTES, regulated upon activation, normal T cell expressed and secreted; sCD40-L, soluble CD40 ligand; sIL-2Rα, soluble IL - 2 receptor α; TGF-α, transforming growth factor α; TNF-α, tumor necrosis factor α; TNF-β, tumor necrosis factor β; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: Cytokine responses measured by 42-multiplex assay. A, Proinflammatory cytokines; B, T helper 2, regulatory, and T cell activation cytokines; C, Chemokines; and D, Growth factors in supernatants from diluted whole-blood cultures that were stimulated with Mycobacterium tuberculosis purified protein derivative for 6 days were measured using a 42-multiplex assay 3 months and 12 months after BCG vaccination in UK and Malawian infants (3 months: UK n = 28, Malawi n = 40; 12 months: UK n = 26, Malawi n = 36). Line represents median response. EGF, epidermal growth factor; FGF-2, fibroblast growth factor 2; Flt3-L, FMS-like tyrosine kinase 3 ligand; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte macrophage colony-stimulating factor; GRO, growth regulated oncogene; IFN-α, interferon α; IFN-γ, interferon-γ; IL-1α, interleukin 1α; IL-1β, interleukin 1β; IL-1RA, interleukin 1receptor antagonist; IL-2, interleukin 2; IL-3, interleukin 3; IL-4, interleukin 4; IL-5, interleukin 5; IL-6, interleukin 6; IL-7, interleukin 7; IL-9, interleukin 9; IL-10, interleukin 10; IL-12p40, interleukin 12p40; IL-12p70, interleukin 12p70; IL-13, interleukin 13; IL-17, interleukin 17; IP-10 (IFN-γ inducible protein 10); MCP-3, monocyte chemotactic protein 3; MDC, macrophage-derived chemokine; MIP-1α, macrophage inflammatory protein 1α; MIP-1β, macrophage inflammatory protein 1β; PDGF-AA, platelet derived growth factor AA; PDGF-AB/BB, platelet derived growth factor AB/BB; RANTES, regulated upon activation, normal T cell expressed and secreted; sCD40-L, soluble CD40 ligand; sIL-2Rα, soluble IL - 2 receptor α; TGF-α, transforming growth factor α; TNF-α, tumor necrosis factor α; TNF-β, tumor necrosis factor β; VEGF, vascular endothelial growth factor.

    Techniques Used: Multiplex Assay, Activation Assay, Purification, Derivative Assay

    25) Product Images from "Polymer-Assisted In Situ Synthesis of Silver Nanoparticles with Epigallocatechin Gallate (EGCG) Impregnated Wound Patch Potentiate Controlled Inflammatory Responses for Brisk Wound Healing"

    Article Title: Polymer-Assisted In Situ Synthesis of Silver Nanoparticles with Epigallocatechin Gallate (EGCG) Impregnated Wound Patch Potentiate Controlled Inflammatory Responses for Brisk Wound Healing

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S228462

    Effect of hydrogel patches on growth factor and cytokines. ( A ) Unaltered expression levels of IFN-γ secretion. ( B and C ) Inhibition of pro-inflammatory cytokines IL-6 and TNF-α secretion on treatment with HG-Ag-EGCG. ( D ) Significant changes found in anti-inflammatory IL-10 secretion on HG-Ag-EGCG treated group ( E ) Enhanced VEGF secretion on HG-Ag-EGCG treated group (n=3, ***p=0.0006, **p
    Figure Legend Snippet: Effect of hydrogel patches on growth factor and cytokines. ( A ) Unaltered expression levels of IFN-γ secretion. ( B and C ) Inhibition of pro-inflammatory cytokines IL-6 and TNF-α secretion on treatment with HG-Ag-EGCG. ( D ) Significant changes found in anti-inflammatory IL-10 secretion on HG-Ag-EGCG treated group ( E ) Enhanced VEGF secretion on HG-Ag-EGCG treated group (n=3, ***p=0.0006, **p

    Techniques Used: Expressing, Inhibition

    26) Product Images from "Acute phase ketosis-prone atypical diabetes is associated with a pro-inflammatory profile: a case-control study in a sub-Saharan African population"

    Article Title: Acute phase ketosis-prone atypical diabetes is associated with a pro-inflammatory profile: a case-control study in a sub-Saharan African population

    Journal: Journal of Diabetes and Metabolic Disorders

    doi: 10.1007/s40200-018-0336-8

    Concentrations of TNF-α ( a ), MCP-1 ( b ), MIP1-α ( c ), IL-8 ( d ), MIP1-β ( e ), and VEGF ( f ) according to the diabetes phenotype (Ketotic phase KPD, non-ketotic phase KPD, and type 2 diabetes). TNF-α: Tumor necrosis factor alpha, MCP-1: Monocyte Chemoattractant Protein-1, MIP1-α: Macrophage inflammatory protein one alpha, IL-8: Interleukin-8, MIP1-β: Macrophage inflammatory protein one beta, VEGF: Vascular endothelial growth factor. KPD: Ketosis prone diabetes. The results are presented as median and interquartile range (25th and 75th). * = p
    Figure Legend Snippet: Concentrations of TNF-α ( a ), MCP-1 ( b ), MIP1-α ( c ), IL-8 ( d ), MIP1-β ( e ), and VEGF ( f ) according to the diabetes phenotype (Ketotic phase KPD, non-ketotic phase KPD, and type 2 diabetes). TNF-α: Tumor necrosis factor alpha, MCP-1: Monocyte Chemoattractant Protein-1, MIP1-α: Macrophage inflammatory protein one alpha, IL-8: Interleukin-8, MIP1-β: Macrophage inflammatory protein one beta, VEGF: Vascular endothelial growth factor. KPD: Ketosis prone diabetes. The results are presented as median and interquartile range (25th and 75th). * = p

    Techniques Used:

    27) Product Images from "Macrophages activate mesenchymal stem cells to acquire cancer-associated fibroblast-like features resulting in gastric epithelial cell lesions and malignant transformation in vitro"

    Article Title: Macrophages activate mesenchymal stem cells to acquire cancer-associated fibroblast-like features resulting in gastric epithelial cell lesions and malignant transformation in vitro

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.9703

    High levels of IL-8, PDGF-B, GM-CSF, IL-6, MCP-1, VEGF and TNF were presented in the supernatant from macrophage-hucMSCs. (A) The expression of cytokines (IL-8, P=0.0092; PDGF-B, P=0.0391; GM-CSF, P=0.0095; IL-6, P=0.0077; MCP-1, P=0.0084; VEGF, P=0.0441; TNF, P=0.0244) in the supernatant of the hucMSCs cultured with or without macrophages were measured using a Luminex assay. (B) mRNA expressions of IL-8, PDGF-B, GM-CSF, IL-6, MCP-1 and TNF significantly increased in the macrophage-hucMSCs. (C) Supernatants from the two aforementioned types of cells were assessed for IL-8, GM-CSF, IL-6 and MCP-1 expression by ELISA. Data are presented as the mean ± standard deviation; *P
    Figure Legend Snippet: High levels of IL-8, PDGF-B, GM-CSF, IL-6, MCP-1, VEGF and TNF were presented in the supernatant from macrophage-hucMSCs. (A) The expression of cytokines (IL-8, P=0.0092; PDGF-B, P=0.0391; GM-CSF, P=0.0095; IL-6, P=0.0077; MCP-1, P=0.0084; VEGF, P=0.0441; TNF, P=0.0244) in the supernatant of the hucMSCs cultured with or without macrophages were measured using a Luminex assay. (B) mRNA expressions of IL-8, PDGF-B, GM-CSF, IL-6, MCP-1 and TNF significantly increased in the macrophage-hucMSCs. (C) Supernatants from the two aforementioned types of cells were assessed for IL-8, GM-CSF, IL-6 and MCP-1 expression by ELISA. Data are presented as the mean ± standard deviation; *P

    Techniques Used: Expressing, Cell Culture, Luminex, Enzyme-linked Immunosorbent Assay, Standard Deviation

    28) Product Images from "In vitro and in vivo evaluation of the anti-angiogenic actions of 4-hydroxybenzyl alcohol"

    Article Title: In vitro and in vivo evaluation of the anti-angiogenic actions of 4-hydroxybenzyl alcohol

    Journal: British Journal of Pharmacology

    doi: 10.1111/j.1476-5381.2011.01292.x

    Western blot analysis of PCNA, VEGF and MMP9 protein expression [shown as optical density (OD) × mm 2 ] of eEND2 cells, which were cultured for 24 h in DMEM supplemented with vehicle (control, white bars), 10 mM (grey bars) or 25 mM (black bars) HBA. Data shown are means ± SEM. * P
    Figure Legend Snippet: Western blot analysis of PCNA, VEGF and MMP9 protein expression [shown as optical density (OD) × mm 2 ] of eEND2 cells, which were cultured for 24 h in DMEM supplemented with vehicle (control, white bars), 10 mM (grey bars) or 25 mM (black bars) HBA. Data shown are means ± SEM. * P

    Techniques Used: Western Blot, Expressing, Cell Culture

    29) Product Images from "Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor"

    Article Title: Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor

    Journal: Stem Cells Translational Medicine

    doi: 10.5966/sctm.2015-0264

    Cerebrovascular cytokines level and gene expression after EPO treatment in cultured endothelial progenitor cells (EPCs). (A): Bar graphs demonstrate the peripheral serum levels of VEGF (Aa) , TNF-α (Ab) , and IL-6 (Ac) . Data are means ±
    Figure Legend Snippet: Cerebrovascular cytokines level and gene expression after EPO treatment in cultured endothelial progenitor cells (EPCs). (A): Bar graphs demonstrate the peripheral serum levels of VEGF (Aa) , TNF-α (Ab) , and IL-6 (Ac) . Data are means ±

    Techniques Used: Expressing, Cell Culture

    30) Product Images from "PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing"

    Article Title: PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-55214-7

    ( A ) Percentage weight loss of CS-PEG scaffold in 1 mg/ml lysozyme in PBS at 37 °C. n = 5. (B) Cumulative percentage in vitro release profile of bFGF and VEGF from CS-PEG scaffold in PBS (pH 7.4) at 37 °C. ( C ) SEM images of (a) CS-PEG scaffolds, (b) growth factor bound CS -PEG scaffolds. ( C ), reproduced in RGCB annual report.
    Figure Legend Snippet: ( A ) Percentage weight loss of CS-PEG scaffold in 1 mg/ml lysozyme in PBS at 37 °C. n = 5. (B) Cumulative percentage in vitro release profile of bFGF and VEGF from CS-PEG scaffold in PBS (pH 7.4) at 37 °C. ( C ) SEM images of (a) CS-PEG scaffolds, (b) growth factor bound CS -PEG scaffolds. ( C ), reproduced in RGCB annual report.

    Techniques Used: In Vitro

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    Synthesized:

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    Article Snippet: .. Bitis arietans primer design Primers complimentary to genes encoding B . arietans serine proteases (SP), snake venom metalloproteinases (SVMPs), L-amino acid oxidases (LAO), C-type lectins (CTL), vascular endothelial growth factors (VEGF) and aminopeptidases (AMP) were designed ( ) and synthesized commercially (Sigma Aldrich). ..

    Metabolic Assay:

    Article Title: Bioengineering Vascular Networks to Study Angiogenesis and Vascularization of Physiologically Relevant Tissue Models in Vitro
    Article Snippet: .. 2.1 Materials 2-Deoxy-d -ribose (2dDR), 37% formaldehyde (FA) solution, 4′,6-diamidino-2-phenylindole (DAPI) solution, adenine, AlamarBlue cell metabolic activity assay, alginic acid sodium salt, amphotericin B, anti-CD31 (PECAM-1) antibody produced in mouse, bovine serum albumin (BSA), calcium chloride dihydrate, chlorotoxin, collagenase A, d -glucose, dimethyl sulfoxide (DMSO), Dulbecco’s modified Eagle’s medium (DMEM), ethylenediaminetetraacetic acid (EDTA), eosin Y solution, ethanol, F-12 HAM nutrient mixture, fetal calf serum (FCS), fibrinogen from human plasma, glutaraldehyde (25%), glycerol, hematoxylin solution, hydrocortisone, insulin (human recombinant), l -glutamine, methylene blue, penicillin/streptomycin, phalloidin, fluorescein isothiocyanate (FITC), phalloidin, tetramethylrhodamine isothiocyanate (TRITC), sodium hydroxide pellets, Trypan blue, trypsin EDTA, Tween20, vascular endothelial growth factor (VEGF), and sodium chloride (NaCl) were purchased from Sigma-Aldrich. .. Dichloromethane (DCM), DPX mounting medium, industrial methylated spirit (IMS), methanol, Triton X-100, and xylene were purchased from Fisher Scientific.

    CTL Assay:

    Article Title: Stabilising the Integrity of Snake Venom mRNA Stored under Tropical Field Conditions Expands Research HorizonsFull-length Venom Protein cDNA Sequences from Venom-derived mRNA: Exploring Compositional Variation and Adaptive Multigene Evolution
    Article Snippet: .. Bitis arietans primer design Primers complimentary to genes encoding B . arietans serine proteases (SP), snake venom metalloproteinases (SVMPs), L-amino acid oxidases (LAO), C-type lectins (CTL), vascular endothelial growth factors (VEGF) and aminopeptidases (AMP) were designed ( ) and synthesized commercially (Sigma Aldrich). ..

    Produced:

    Article Title: Bioengineering Vascular Networks to Study Angiogenesis and Vascularization of Physiologically Relevant Tissue Models in Vitro
    Article Snippet: .. 2.1 Materials 2-Deoxy-d -ribose (2dDR), 37% formaldehyde (FA) solution, 4′,6-diamidino-2-phenylindole (DAPI) solution, adenine, AlamarBlue cell metabolic activity assay, alginic acid sodium salt, amphotericin B, anti-CD31 (PECAM-1) antibody produced in mouse, bovine serum albumin (BSA), calcium chloride dihydrate, chlorotoxin, collagenase A, d -glucose, dimethyl sulfoxide (DMSO), Dulbecco’s modified Eagle’s medium (DMEM), ethylenediaminetetraacetic acid (EDTA), eosin Y solution, ethanol, F-12 HAM nutrient mixture, fetal calf serum (FCS), fibrinogen from human plasma, glutaraldehyde (25%), glycerol, hematoxylin solution, hydrocortisone, insulin (human recombinant), l -glutamine, methylene blue, penicillin/streptomycin, phalloidin, fluorescein isothiocyanate (FITC), phalloidin, tetramethylrhodamine isothiocyanate (TRITC), sodium hydroxide pellets, Trypan blue, trypsin EDTA, Tween20, vascular endothelial growth factor (VEGF), and sodium chloride (NaCl) were purchased from Sigma-Aldrich. .. Dichloromethane (DCM), DPX mounting medium, industrial methylated spirit (IMS), methanol, Triton X-100, and xylene were purchased from Fisher Scientific.

    Activation Assay:

    Article Title: In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures
    Article Snippet: .. Secretion of granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interferon gamma inducible protein 10 (IP-10), interleukin (IL)-1α, IL-1β, IL-6, IL-8, monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor (VEGF), eotaxin, regulated on activation, normal T cell expressed and secreted (RANTES) (Milliplex MAP Human cytokine/chemokine magnetic bead panel, HCYTOMAG-60K, Millipore) and MMP-1 and MMP-9 (Milliplex MAP Human MMP magnetic bead panel 2, HMMP2MAG-55K, Millipore) were measured by Luminex-based technology following the technical recommendations of Milliplex (Millipore). .. As a positive control test, the buccal and gingival tissues (N = 3 inserts/tissue) were treated with a combination of tumor necrosis factor (TNF)-α + IL-1β in the basolateral medium, for 24 h at 37 °C and 5% CO2 (data not shown).

    other:

    Article Title: Differential response of rat strains to obesogenic diets underlines the importance of genetic makeup of an individual towards obesity
    Article Snippet: Rat Cytokine/Chemokine Profile The adipocytokines such as leptin, macrophage inflammatory protein-1 alpha (MIP-1α, CCL3), interleukin-4 (IL-4), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), macrophage chemo attractant protein-1 (MCP-1, CCL2), IFN-gamma-inducible protein 10 (IP- 10, CXCL10), vascular endothelial growth factor (VEGF), and tumor necrosis factor-alpha (TNF-α) were estimated both in circulation as well as in visceral adipose tissue by Milliplex Rat cytokine immunoassay kit (Millipore, USA) according to manufacturer’s instructions.

    Article Title: In utero exposure to ultrafine particles promotes placental stress-induced programming of renin-angiotensin system-related elements in the offspring results in altered blood pressure in adult mice
    Article Snippet: Analysis of cytokines and chemokines The pro-inflammatory cytokines interleukin-β (IL-β), IL-6 and tumor necrosis factor-α (TNF-α), the anti-inflammatory cytokines IL-10 and IL-4, chemokines including neutrophil chemoattractant (KC), macrophage inflammatory protein 2 (MIP-2) and monocyte chemoattractant protein 1 (MCP-1), and vascular endothelial growth factor (VEGF) were measured in the dam sera and the total protein of the dam lungs, placentas and fetuses using an MCYTOMAG-70 K Milliplex MAP Mouse Cytokine/Chemokine magnetic bead panel (EMD Millipore, Billerica, MA, USA) following the manufacturer’s instructions.

    Modification:

    Article Title: Bioengineering Vascular Networks to Study Angiogenesis and Vascularization of Physiologically Relevant Tissue Models in Vitro
    Article Snippet: .. 2.1 Materials 2-Deoxy-d -ribose (2dDR), 37% formaldehyde (FA) solution, 4′,6-diamidino-2-phenylindole (DAPI) solution, adenine, AlamarBlue cell metabolic activity assay, alginic acid sodium salt, amphotericin B, anti-CD31 (PECAM-1) antibody produced in mouse, bovine serum albumin (BSA), calcium chloride dihydrate, chlorotoxin, collagenase A, d -glucose, dimethyl sulfoxide (DMSO), Dulbecco’s modified Eagle’s medium (DMEM), ethylenediaminetetraacetic acid (EDTA), eosin Y solution, ethanol, F-12 HAM nutrient mixture, fetal calf serum (FCS), fibrinogen from human plasma, glutaraldehyde (25%), glycerol, hematoxylin solution, hydrocortisone, insulin (human recombinant), l -glutamine, methylene blue, penicillin/streptomycin, phalloidin, fluorescein isothiocyanate (FITC), phalloidin, tetramethylrhodamine isothiocyanate (TRITC), sodium hydroxide pellets, Trypan blue, trypsin EDTA, Tween20, vascular endothelial growth factor (VEGF), and sodium chloride (NaCl) were purchased from Sigma-Aldrich. .. Dichloromethane (DCM), DPX mounting medium, industrial methylated spirit (IMS), methanol, Triton X-100, and xylene were purchased from Fisher Scientific.

    Recombinant:

    Article Title: Bioengineering Vascular Networks to Study Angiogenesis and Vascularization of Physiologically Relevant Tissue Models in Vitro
    Article Snippet: .. 2.1 Materials 2-Deoxy-d -ribose (2dDR), 37% formaldehyde (FA) solution, 4′,6-diamidino-2-phenylindole (DAPI) solution, adenine, AlamarBlue cell metabolic activity assay, alginic acid sodium salt, amphotericin B, anti-CD31 (PECAM-1) antibody produced in mouse, bovine serum albumin (BSA), calcium chloride dihydrate, chlorotoxin, collagenase A, d -glucose, dimethyl sulfoxide (DMSO), Dulbecco’s modified Eagle’s medium (DMEM), ethylenediaminetetraacetic acid (EDTA), eosin Y solution, ethanol, F-12 HAM nutrient mixture, fetal calf serum (FCS), fibrinogen from human plasma, glutaraldehyde (25%), glycerol, hematoxylin solution, hydrocortisone, insulin (human recombinant), l -glutamine, methylene blue, penicillin/streptomycin, phalloidin, fluorescein isothiocyanate (FITC), phalloidin, tetramethylrhodamine isothiocyanate (TRITC), sodium hydroxide pellets, Trypan blue, trypsin EDTA, Tween20, vascular endothelial growth factor (VEGF), and sodium chloride (NaCl) were purchased from Sigma-Aldrich. .. Dichloromethane (DCM), DPX mounting medium, industrial methylated spirit (IMS), methanol, Triton X-100, and xylene were purchased from Fisher Scientific.

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  • 93
    Millipore vegf a
    Effects of ABL on <t>VEGF-induced</t> HUVEC migration and tube formation. A-B) ABL increases VEGF-induced ECs transwell migration. The data represent the number of migrated cells per microscopic field in VEGF-treated cells vs. control cells. C-D) ABL increases VEGF-induced EC transwell migration. Confluent HUVECs in a monolayer were pretreated with ABL or vehicle for 2 h and wounded with a cell scraper. After 48 h of incubation at 37°C, the number of cells that migrated across the wound edge was counted in each field. E-F) ABL increases VEGF-induced EC tube formation. The images were visualized using a phase contrast microscope (10× magnification). The total tubule length from 10 non-overlapping fields was measured by tracing the tube-like structure. The values represent the mean ± SEM from 3 independent experiments ( n = 3). Scale bar: 100 μm.
    Vegf A, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore vegf mrna
    <t>VEGF</t> siRNA-generating pDNA down regulates VEGF <t>mRNA</t> levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p
    Vegf Mrna, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore p vegfr 1
    Ligand-induced activation <t>VEGFR-1/-3</t> activation and downstream AKT and ERK activation by immunoblotting. A, SW480 cells cultured in serum-free media were pretreated with or without cediranib (100 nmol/L), were left untreated (SF; lanes 1, 6 ), or were
    P Vegfr 1, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of ABL on VEGF-induced HUVEC migration and tube formation. A-B) ABL increases VEGF-induced ECs transwell migration. The data represent the number of migrated cells per microscopic field in VEGF-treated cells vs. control cells. C-D) ABL increases VEGF-induced EC transwell migration. Confluent HUVECs in a monolayer were pretreated with ABL or vehicle for 2 h and wounded with a cell scraper. After 48 h of incubation at 37°C, the number of cells that migrated across the wound edge was counted in each field. E-F) ABL increases VEGF-induced EC tube formation. The images were visualized using a phase contrast microscope (10× magnification). The total tubule length from 10 non-overlapping fields was measured by tracing the tube-like structure. The values represent the mean ± SEM from 3 independent experiments ( n = 3). Scale bar: 100 μm.

    Journal: PLoS ONE

    Article Title: Acetylbritannilactone Modulates Vascular Endothelial Growth Factor Signaling and Regulates Angiogenesis in Endothelial Cells

    doi: 10.1371/journal.pone.0148968

    Figure Lengend Snippet: Effects of ABL on VEGF-induced HUVEC migration and tube formation. A-B) ABL increases VEGF-induced ECs transwell migration. The data represent the number of migrated cells per microscopic field in VEGF-treated cells vs. control cells. C-D) ABL increases VEGF-induced EC transwell migration. Confluent HUVECs in a monolayer were pretreated with ABL or vehicle for 2 h and wounded with a cell scraper. After 48 h of incubation at 37°C, the number of cells that migrated across the wound edge was counted in each field. E-F) ABL increases VEGF-induced EC tube formation. The images were visualized using a phase contrast microscope (10× magnification). The total tubule length from 10 non-overlapping fields was measured by tracing the tube-like structure. The values represent the mean ± SEM from 3 independent experiments ( n = 3). Scale bar: 100 μm.

    Article Snippet: The next day, pretreated the cells with vehicle or different doses of ABL, followed by exposure of some of the wells (n = 16) to VEGF-A (50 ng/mL) for 48 h. Cell growth was assessed using an MTT assay (Millipore Corporation, Temecula, CA, USA) according to the manufacturer’s protocol.

    Techniques: Migration, Incubation, Microscopy

    Schematic representation of the ABL effect on VEGF signaling in ECs. ABL inhibits VEGFR-2 and VE-cadherin association, thus promoting VEGFR-2 internalization, following by VEGF-induced VEGFR-2 phosphorylation and downstream signaling.

    Journal: PLoS ONE

    Article Title: Acetylbritannilactone Modulates Vascular Endothelial Growth Factor Signaling and Regulates Angiogenesis in Endothelial Cells

    doi: 10.1371/journal.pone.0148968

    Figure Lengend Snippet: Schematic representation of the ABL effect on VEGF signaling in ECs. ABL inhibits VEGFR-2 and VE-cadherin association, thus promoting VEGFR-2 internalization, following by VEGF-induced VEGFR-2 phosphorylation and downstream signaling.

    Article Snippet: The next day, pretreated the cells with vehicle or different doses of ABL, followed by exposure of some of the wells (n = 16) to VEGF-A (50 ng/mL) for 48 h. Cell growth was assessed using an MTT assay (Millipore Corporation, Temecula, CA, USA) according to the manufacturer’s protocol.

    Techniques:

    ABL enhances VEGF signaling in endothelial cells. A) HUVECs were serum-starved overnight and pretreated with ABL or vehicle for 2 h, followed by exposure to VEGF-A (50 ng/mL) for various times as indicated. MAPK MAPK p44/42 Thr202/Tyr204 , p38 Thr180/Tyr182 , and Akt Ser473 phosphorylation in the cell lysates were measured via western blot analysis. B-D) The quantitative analysis represents the ratio of phosphorylated/total MAPK MAPK p44/42, MAPK p38, and Akt from 3 independent experiments. E) VEGFR-2 Tyr1175 phosphorylation in the cell lysates was measured via western blot analysis. F) Quantitative analysis of the ratio of phosphorylated/total VEGFR-2 from three independent experiments ( n = 3).

    Journal: PLoS ONE

    Article Title: Acetylbritannilactone Modulates Vascular Endothelial Growth Factor Signaling and Regulates Angiogenesis in Endothelial Cells

    doi: 10.1371/journal.pone.0148968

    Figure Lengend Snippet: ABL enhances VEGF signaling in endothelial cells. A) HUVECs were serum-starved overnight and pretreated with ABL or vehicle for 2 h, followed by exposure to VEGF-A (50 ng/mL) for various times as indicated. MAPK MAPK p44/42 Thr202/Tyr204 , p38 Thr180/Tyr182 , and Akt Ser473 phosphorylation in the cell lysates were measured via western blot analysis. B-D) The quantitative analysis represents the ratio of phosphorylated/total MAPK MAPK p44/42, MAPK p38, and Akt from 3 independent experiments. E) VEGFR-2 Tyr1175 phosphorylation in the cell lysates was measured via western blot analysis. F) Quantitative analysis of the ratio of phosphorylated/total VEGFR-2 from three independent experiments ( n = 3).

    Article Snippet: The next day, pretreated the cells with vehicle or different doses of ABL, followed by exposure of some of the wells (n = 16) to VEGF-A (50 ng/mL) for 48 h. Cell growth was assessed using an MTT assay (Millipore Corporation, Temecula, CA, USA) according to the manufacturer’s protocol.

    Techniques: Western Blot

    ABL increases VEGF-induced HUVEC growth and proliferation. A) VEGF-induced EC growth monitored by the MTT assay following treatment with different doses of ABL or vehicle. The data represent the percent increase after 48 h relative to non-stimulated cells from 3 independent experiments. B) VEGF-induced [ 3 H]-thymidine incorporation following different doses of ABL or vehicle. The data represent the percent increase after 48 h relative to the non-stimulated cells from 3 independent experiments ( n = 3). * P

    Journal: PLoS ONE

    Article Title: Acetylbritannilactone Modulates Vascular Endothelial Growth Factor Signaling and Regulates Angiogenesis in Endothelial Cells

    doi: 10.1371/journal.pone.0148968

    Figure Lengend Snippet: ABL increases VEGF-induced HUVEC growth and proliferation. A) VEGF-induced EC growth monitored by the MTT assay following treatment with different doses of ABL or vehicle. The data represent the percent increase after 48 h relative to non-stimulated cells from 3 independent experiments. B) VEGF-induced [ 3 H]-thymidine incorporation following different doses of ABL or vehicle. The data represent the percent increase after 48 h relative to the non-stimulated cells from 3 independent experiments ( n = 3). * P

    Article Snippet: The next day, pretreated the cells with vehicle or different doses of ABL, followed by exposure of some of the wells (n = 16) to VEGF-A (50 ng/mL) for 48 h. Cell growth was assessed using an MTT assay (Millipore Corporation, Temecula, CA, USA) according to the manufacturer’s protocol.

    Techniques: MTT Assay

    ABL enhances VEGFR-2 internalization in ECs. A) Colocalization of VEGFR-2 with endosome markers EEA1 in ECs. The ECs were pretreated with ABL for 2 h following fifteen minutes of VEGF stimulation, fixed, permeablized, and labeled with anti-VEGFR-2 (red) and anti-EEA1 (green) and processed for confocal microscopy. The endocytic trafficking of VEGFR2 without locating in endosome was observed in ECs (arrow). B) Colocalization of VEGFR-2 (red) and VE-cadherin (green) was observed in ECs (arrow). Nuclei were counterstained with DAPI (blue). Scale bar = 20 μm.

    Journal: PLoS ONE

    Article Title: Acetylbritannilactone Modulates Vascular Endothelial Growth Factor Signaling and Regulates Angiogenesis in Endothelial Cells

    doi: 10.1371/journal.pone.0148968

    Figure Lengend Snippet: ABL enhances VEGFR-2 internalization in ECs. A) Colocalization of VEGFR-2 with endosome markers EEA1 in ECs. The ECs were pretreated with ABL for 2 h following fifteen minutes of VEGF stimulation, fixed, permeablized, and labeled with anti-VEGFR-2 (red) and anti-EEA1 (green) and processed for confocal microscopy. The endocytic trafficking of VEGFR2 without locating in endosome was observed in ECs (arrow). B) Colocalization of VEGFR-2 (red) and VE-cadherin (green) was observed in ECs (arrow). Nuclei were counterstained with DAPI (blue). Scale bar = 20 μm.

    Article Snippet: The next day, pretreated the cells with vehicle or different doses of ABL, followed by exposure of some of the wells (n = 16) to VEGF-A (50 ng/mL) for 48 h. Cell growth was assessed using an MTT assay (Millipore Corporation, Temecula, CA, USA) according to the manufacturer’s protocol.

    Techniques: Labeling, Confocal Microscopy

    ABL enhances Matrigel angiogenesis in vivo . A) Examples of CD31 immunofluorescence staining of Matrigel plugs containing VEGF-A or control buffer. B) The quantification data represent the percent increase in CD31-positive area in comparison with the control sample ( n = 6). C) Quantitative analysis of GAPDH-normalized VE-cadherin ( Cdh5 ) mRNA expression in Matrigel plugs containing VEGF-A or control buffer that were implanted in mice ( n = 3). Scale bars: 100 μm.

    Journal: PLoS ONE

    Article Title: Acetylbritannilactone Modulates Vascular Endothelial Growth Factor Signaling and Regulates Angiogenesis in Endothelial Cells

    doi: 10.1371/journal.pone.0148968

    Figure Lengend Snippet: ABL enhances Matrigel angiogenesis in vivo . A) Examples of CD31 immunofluorescence staining of Matrigel plugs containing VEGF-A or control buffer. B) The quantification data represent the percent increase in CD31-positive area in comparison with the control sample ( n = 6). C) Quantitative analysis of GAPDH-normalized VE-cadherin ( Cdh5 ) mRNA expression in Matrigel plugs containing VEGF-A or control buffer that were implanted in mice ( n = 3). Scale bars: 100 μm.

    Article Snippet: The next day, pretreated the cells with vehicle or different doses of ABL, followed by exposure of some of the wells (n = 16) to VEGF-A (50 ng/mL) for 48 h. Cell growth was assessed using an MTT assay (Millipore Corporation, Temecula, CA, USA) according to the manufacturer’s protocol.

    Techniques: In Vivo, Immunofluorescence, Staining, Expressing, Mouse Assay

    Inhibition of VEGF-A-mediated PI3K/Akt pathway in HUVECs. The HUVECs were serum-starved for 24 h and stimulated in fresh medium with VEGF-A (25 ng/ml) and then treated with HY (0.062 μM) in combination with VEGF-A (25 ng/ml) for 24 h. The cells were exposed to a 585-nm LED light at a dose of 1.0 J/cm 2 and were incubation for 24 h. The cells were lysed and the proteins were harvested for western blot analysis of VEGF-A ( a ), p-Akt (Ser473) and Akt ( b ), and Bad ( c ). Densitometric measurements were analysed using AlphaEaseFC 4.0 software. The protein expression levels were normalized to those of the vehicle control (100%). Data are presented as means ± S.D. (n = 3); ** P

    Journal: Scientific Reports

    Article Title: Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis

    doi: 10.1038/srep18398

    Figure Lengend Snippet: Inhibition of VEGF-A-mediated PI3K/Akt pathway in HUVECs. The HUVECs were serum-starved for 24 h and stimulated in fresh medium with VEGF-A (25 ng/ml) and then treated with HY (0.062 μM) in combination with VEGF-A (25 ng/ml) for 24 h. The cells were exposed to a 585-nm LED light at a dose of 1.0 J/cm 2 and were incubation for 24 h. The cells were lysed and the proteins were harvested for western blot analysis of VEGF-A ( a ), p-Akt (Ser473) and Akt ( b ), and Bad ( c ). Densitometric measurements were analysed using AlphaEaseFC 4.0 software. The protein expression levels were normalized to those of the vehicle control (100%). Data are presented as means ± S.D. (n = 3); ** P

    Article Snippet: The proteins were separated by 10% or 12% (for Bcl-2, Bax, cleaved caspase-9, procaspase-3, cleaved PARP, VEGF-A, phospho-Akt (Ser473, p-Akt), Akt, Bad and β-Actin) SDS-polyacrylamide gels and electrophoretically transferred onto PVDF membranes (Millipore, Massachusetts, USA).

    Techniques: Inhibition, Incubation, Western Blot, Software, Expressing

    VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis

    doi: 10.1186/1477-7827-6-64

    Figure Lengend Snippet: VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat cervix . The VEGF gene silencing efficiency of the selected siRNA-generating pDNA was tested in vivo in the cervix of pregnant rat from GD17–20. VEGF siRNA-generating pDNA was found to down-regulate VEGF compared to control. n = 5, ( p

    Article Snippet: Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below.

    Techniques: In Vivo

    VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat heart fibroblasts . The VEGF gene silencing efficiency by the three siRNA-generating pDNAs were tested in vitro using rat heart fibroblast primary cell transfection. VEGF siRNA-generating pDNA was found to down-regulate VEGF in cultured rat heart fibroblasts compared to control. The pDNA with the best silencing efficiency was selected and used in the rat cervix to test its efficiency to down-regulate local cervical VEGF in vivo (see Figure 2). n = 5, ( p

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Delineation of VEGF-regulated genes and functions in the cervix of pregnant rodents by DNA microarray analysis

    doi: 10.1186/1477-7827-6-64

    Figure Lengend Snippet: VEGF siRNA-generating pDNA down regulates VEGF mRNA levels in rat heart fibroblasts . The VEGF gene silencing efficiency by the three siRNA-generating pDNAs were tested in vitro using rat heart fibroblast primary cell transfection. VEGF siRNA-generating pDNA was found to down-regulate VEGF in cultured rat heart fibroblasts compared to control. The pDNA with the best silencing efficiency was selected and used in the rat cervix to test its efficiency to down-regulate local cervical VEGF in vivo (see Figure 2). n = 5, ( p

    Article Snippet: Cervices were harvested, processed and evaluated for changes in levels of VEGF mRNA and whole genome gene expression using DNA microarray data, as described below. b) Pregnant mice and ovariectomized non-pregnant rats and mice treated with either mouse recombinant VEGF protein (10 ng/mouse recombinant VEGF; Calbiochem, La Jolla, CA, once daily from GD13–17, intra-vaginally), VEGF receptor antagonist (as described earlier, but treated daily), or vehicle only, were utilized to confirm the DNA microarray data above using real-time PCR, and for morphological studies (SEM), described below.

    Techniques: In Vitro, Transfection, Cell Culture, In Vivo

    Ligand-induced activation VEGFR-1/-3 activation and downstream AKT and ERK activation by immunoblotting. A, SW480 cells cultured in serum-free media were pretreated with or without cediranib (100 nmol/L), were left untreated (SF; lanes 1, 6 ), or were

    Journal: Molecular cancer therapeutics

    Article Title: Targeting vascular endothelial growth factor receptor-1 and -3 with cediranib (AZD2171): effects on migration and invasion of gastrointestinal cancer cell lines

    doi: 10.1158/1535-7163.MCT-09-0380

    Figure Lengend Snippet: Ligand-induced activation VEGFR-1/-3 activation and downstream AKT and ERK activation by immunoblotting. A, SW480 cells cultured in serum-free media were pretreated with or without cediranib (100 nmol/L), were left untreated (SF; lanes 1, 6 ), or were

    Article Snippet: Phospho-specific antibodies were used to detect p-VEGFR-1 (Upstate, Millipore Corporation; 1:1,000), p-AKT, and p-ERK (Cell Signaling; 1:1,000) by standard immunoblotting methods as described above.

    Techniques: Activation Assay, Cell Culture