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Abcam vascular endothelial growth factor vegf
Schematic of superior cardioprotection, including antiapoptosis and angiogenesis, mediated by exosomal miR‐2 1 secreted by EnMSCs. Abbreviations: Bax, <t>Bcl‐2‐associated</t> X protein; Bcl‐2, B‐cell CLL/lymphoma 2; Casp‐3, caspase‐3; EnMSCs, endometrium‐derived mesenchymal stem cells; miR‐21, microRNA‐21; p‐Akt, phosphorylated Akt; PTEN, phosphatase and tensin homolog; VEFG, vascular endothelial growth factor.
Vascular Endothelial Growth Factor Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA‐21"

Article Title: Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA‐21

Journal: Stem Cells Translational Medicine

doi: 10.5966/sctm.2015-0386

Schematic of superior cardioprotection, including antiapoptosis and angiogenesis, mediated by exosomal miR‐2 1 secreted by EnMSCs. Abbreviations: Bax, Bcl‐2‐associated X protein; Bcl‐2, B‐cell CLL/lymphoma 2; Casp‐3, caspase‐3; EnMSCs, endometrium‐derived mesenchymal stem cells; miR‐21, microRNA‐21; p‐Akt, phosphorylated Akt; PTEN, phosphatase and tensin homolog; VEFG, vascular endothelial growth factor.
Figure Legend Snippet: Schematic of superior cardioprotection, including antiapoptosis and angiogenesis, mediated by exosomal miR‐2 1 secreted by EnMSCs. Abbreviations: Bax, Bcl‐2‐associated X protein; Bcl‐2, B‐cell CLL/lymphoma 2; Casp‐3, caspase‐3; EnMSCs, endometrium‐derived mesenchymal stem cells; miR‐21, microRNA‐21; p‐Akt, phosphorylated Akt; PTEN, phosphatase and tensin homolog; VEFG, vascular endothelial growth factor.

Techniques Used: Derivative Assay

2) Product Images from "Fetal kidney stem cells ameliorate cisplatin induced acute renal failure and promote renal angiogenesis"

Article Title: Fetal kidney stem cells ameliorate cisplatin induced acute renal failure and promote renal angiogenesis

Journal: World Journal of Stem Cells

doi: 10.4252/wjsc.v7.i4.776

Early angiogenic effect of administered fetal kidney stem cells in cisplatin injured kidney. Representative immunoblots showing the expression of hypoxia-inducible factor (HIF)-1α, vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) in saline and fetal kidney stem cells (fKSC) treated groups on day 3 after fKSC therapy (A-D). Bar diagrams showing densitometric quantification of the expression of HIF-1α (B), VEGF (C) and eNOS (D). Comparative gene expression ratio was calculated by referring each gene to β-actin as an internal control. Densitometric analysis applied for comparison of relative protein expression and represented in densitometric arbitrary units (a. u.). Values expressed mean ± SE. a P
Figure Legend Snippet: Early angiogenic effect of administered fetal kidney stem cells in cisplatin injured kidney. Representative immunoblots showing the expression of hypoxia-inducible factor (HIF)-1α, vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) in saline and fetal kidney stem cells (fKSC) treated groups on day 3 after fKSC therapy (A-D). Bar diagrams showing densitometric quantification of the expression of HIF-1α (B), VEGF (C) and eNOS (D). Comparative gene expression ratio was calculated by referring each gene to β-actin as an internal control. Densitometric analysis applied for comparison of relative protein expression and represented in densitometric arbitrary units (a. u.). Values expressed mean ± SE. a P

Techniques Used: Western Blot, Expressing

3) Product Images from "Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model"

Article Title: Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model

Journal: Stem Cells International

doi: 10.1155/2014/316214

Proposed mechanisms underlying the therapeutic actions of TRPC1 knockdown via Lipofectamine delivered siRNA against hypoxia-induced pulmonary arterial hypertension (PAH) in a murine model of pulmonary arterial hypertension (PAH). α -SMA: alpha-smooth muscle actin; BMP-2: bone morphogenetic protein-2; c-Csp3: cleaved caspase-3; c-PARP: cleaved poly(ADP-ribose) polymerase; eNOS: endothelial nitric oxide synthase; ET-1: endothelin-1; HIF-1 α : hypoxia-inducible factor 1-alpha; HW: heart weight; MHC: myosin heavy chain; mito-Bax: mitochondrial Bax; MMP-9: matrix metalloproteinase-9; p-Smad: phosphorylated Smad; RVSP: right ventricle systolic pressure; TGF- β : transforming growth factor beta; TNF- α : tumor necrosis factor alpha; TRPC: transient receptor potential cation channel; VEGF: vascular endothelial growth factor.
Figure Legend Snippet: Proposed mechanisms underlying the therapeutic actions of TRPC1 knockdown via Lipofectamine delivered siRNA against hypoxia-induced pulmonary arterial hypertension (PAH) in a murine model of pulmonary arterial hypertension (PAH). α -SMA: alpha-smooth muscle actin; BMP-2: bone morphogenetic protein-2; c-Csp3: cleaved caspase-3; c-PARP: cleaved poly(ADP-ribose) polymerase; eNOS: endothelial nitric oxide synthase; ET-1: endothelin-1; HIF-1 α : hypoxia-inducible factor 1-alpha; HW: heart weight; MHC: myosin heavy chain; mito-Bax: mitochondrial Bax; MMP-9: matrix metalloproteinase-9; p-Smad: phosphorylated Smad; RVSP: right ventricle systolic pressure; TGF- β : transforming growth factor beta; TNF- α : tumor necrosis factor alpha; TRPC: transient receptor potential cation channel; VEGF: vascular endothelial growth factor.

Techniques Used:

Protein expressions of TRPCs, HIF-1 α , and VEGF of lung parenchyma by day 28 after hypoxia-induced pulmonary arterial hypertension (PAH) ( n = 10). (a) Protein expression of TRPC1, ∗ versus other groups with different symbols (∗, †, ‡), P
Figure Legend Snippet: Protein expressions of TRPCs, HIF-1 α , and VEGF of lung parenchyma by day 28 after hypoxia-induced pulmonary arterial hypertension (PAH) ( n = 10). (a) Protein expression of TRPC1, ∗ versus other groups with different symbols (∗, †, ‡), P

Techniques Used: Expressing

4) Product Images from "3D Spheroids of Umbilical Cord Blood MSC-Derived Schwann Cells Promote Peripheral Nerve Regeneration"

Article Title: 3D Spheroids of Umbilical Cord Blood MSC-Derived Schwann Cells Promote Peripheral Nerve Regeneration

Journal: Frontiers in Cell and Developmental Biology

doi: 10.3389/fcell.2020.604946

SCLCs were assembled into three-dimensional (3D) spheroids to enhance their therapeutic potential. Immunofluorescence evaluation of the expression of (A) SC markers S100 and GFAP, (B) extracellular matrix proteins laminin and collagen, and (C) neurotrophic factor BDNF and proangiogenic factor VEGF. Scale bars: 100 μm. (D) The concentrations of BDNF and VEGF in the collected 3D SCLC spheroids or SCLC suspensions obtained using conventional trypsinization were determined by ELISA ( n = 6, mean ± s.d.). (E) The attachment and proliferation of 3D SCLC spheroids or SCLC suspensions that were harvested and transferred to culture plates were evaluated by using immunofluorescence staining. Scale bars: 100 μm. (F) The spreading of 3D SCLC spheroids over culture plate as time progressed. Scale bars: 1 mm; scale bar in zoom box: 100 μm. **** p
Figure Legend Snippet: SCLCs were assembled into three-dimensional (3D) spheroids to enhance their therapeutic potential. Immunofluorescence evaluation of the expression of (A) SC markers S100 and GFAP, (B) extracellular matrix proteins laminin and collagen, and (C) neurotrophic factor BDNF and proangiogenic factor VEGF. Scale bars: 100 μm. (D) The concentrations of BDNF and VEGF in the collected 3D SCLC spheroids or SCLC suspensions obtained using conventional trypsinization were determined by ELISA ( n = 6, mean ± s.d.). (E) The attachment and proliferation of 3D SCLC spheroids or SCLC suspensions that were harvested and transferred to culture plates were evaluated by using immunofluorescence staining. Scale bars: 100 μm. (F) The spreading of 3D SCLC spheroids over culture plate as time progressed. Scale bars: 1 mm; scale bar in zoom box: 100 μm. **** p

Techniques Used: Immunofluorescence, Expressing, Enzyme-linked Immunosorbent Assay, Staining

5) Product Images from "Salvianolic Acid A Inhibits OX-LDL Effects on Exacerbating Choroidal Neovascularization via Downregulating CYLD"

Article Title: Salvianolic Acid A Inhibits OX-LDL Effects on Exacerbating Choroidal Neovascularization via Downregulating CYLD

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2017/6210694

Real-time PCR analysis of VEGE, PDGF, PEDF, and angiostatin mRNA expression in four groups 7 days after laser. (a, b) OX-LDL injection increased both VEGF and PDGF mRNA expression compared with the PBS group, while OX-LDL + Sal A group reduced VEGF and PDGF mRNA expression compared with the OX-LDL group. (c) There was no difference between each group in PEDF gene expression. (d) The OX-LDL + Sal A group and Sal A group increased angiostatin gene expression compared with the PBS and OX-LDL groups. Data were expressed as mean ± SEM ( n = 10 eyes/group). ∗ P
Figure Legend Snippet: Real-time PCR analysis of VEGE, PDGF, PEDF, and angiostatin mRNA expression in four groups 7 days after laser. (a, b) OX-LDL injection increased both VEGF and PDGF mRNA expression compared with the PBS group, while OX-LDL + Sal A group reduced VEGF and PDGF mRNA expression compared with the OX-LDL group. (c) There was no difference between each group in PEDF gene expression. (d) The OX-LDL + Sal A group and Sal A group increased angiostatin gene expression compared with the PBS and OX-LDL groups. Data were expressed as mean ± SEM ( n = 10 eyes/group). ∗ P

Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Injection

Angiogenesis proteins expression in ARPE-19 cells after OX-LDL and Sal A treatment. ARPE-19 cells were divided into control, OX-LDL(100 mg/L), and OX-LDL (100 mg/L) + Sal A (5 μ M/50 μ M) groups and cultured for 48 hours. (a) Western blot showing changes in VEGF, PDGF, PEDF, and antiangiostatin in ARPE-19 cells 48 hours after treatment. (b–e) Quantitative densitometry results showing that OX-LDL increased VEGF and PDGF levels compared with the control group, while the OX-LDL + Sal A group decreased VEGF/PDGF and slightly increased antiangiostatin level compared with the OX-LDL group. There was no significant difference between each group in PEDF level. Data were expressed as mean ± SEM. ∗ P
Figure Legend Snippet: Angiogenesis proteins expression in ARPE-19 cells after OX-LDL and Sal A treatment. ARPE-19 cells were divided into control, OX-LDL(100 mg/L), and OX-LDL (100 mg/L) + Sal A (5 μ M/50 μ M) groups and cultured for 48 hours. (a) Western blot showing changes in VEGF, PDGF, PEDF, and antiangiostatin in ARPE-19 cells 48 hours after treatment. (b–e) Quantitative densitometry results showing that OX-LDL increased VEGF and PDGF levels compared with the control group, while the OX-LDL + Sal A group decreased VEGF/PDGF and slightly increased antiangiostatin level compared with the OX-LDL group. There was no significant difference between each group in PEDF level. Data were expressed as mean ± SEM. ∗ P

Techniques Used: Expressing, Cell Culture, Western Blot

6) Product Images from "Paradoxical impairment of angiogenesis, endothelial function and circulating number of endothelial progenitor cells in DPP4-deficient rat after critical limb ischemia"

Article Title: Paradoxical impairment of angiogenesis, endothelial function and circulating number of endothelial progenitor cells in DPP4-deficient rat after critical limb ischemia

Journal: Stem Cell Research & Therapy

doi: 10.1186/scrt181

Proposed mechanisms underlying the effects of DPP4-deficient on impairment of angiogenesis, endothelial function and circulating endothelial progenitor cell number based on the findings of the present study . eNOS, endothelial nitric oxide synthase; EPC, endothelial progenitor cell; G-CSF, granulocyte colony-stimulating factor; NO, nitric oxide; SDF, stromal cell-derived factor; VEGF, vascular endothelial growth factor; vWF, von Willebrand factor.
Figure Legend Snippet: Proposed mechanisms underlying the effects of DPP4-deficient on impairment of angiogenesis, endothelial function and circulating endothelial progenitor cell number based on the findings of the present study . eNOS, endothelial nitric oxide synthase; EPC, endothelial progenitor cell; G-CSF, granulocyte colony-stimulating factor; NO, nitric oxide; SDF, stromal cell-derived factor; VEGF, vascular endothelial growth factor; vWF, von Willebrand factor.

Techniques Used: Derivative Assay

7) Product Images from "G9a Suppression Alleviates Corneal Neovascularization through Blocking Nox4-Mediated Oxidative Stress"

Article Title: G9a Suppression Alleviates Corneal Neovascularization through Blocking Nox4-Mediated Oxidative Stress

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2020/6983268

Treatment with BIX 01294 reduced angiogenesis and oxidative stress induced by alkali burn in the cornea. Western blot was performed for the expression of G9a and VEGF (a-c) after treatment with BIX (20 mg/kg, once daily) and quantification of their expression in fold change relative to the control group. SOD activity (d), MDA content (e), and H2O2 production (f) were also detected after treatment with BIX. The mRNA levels of proangiogenic factors were also determined by quantitative real-time PCR (g-l). Data are expressed as means ± SD ( n = 5). ∗ P
Figure Legend Snippet: Treatment with BIX 01294 reduced angiogenesis and oxidative stress induced by alkali burn in the cornea. Western blot was performed for the expression of G9a and VEGF (a-c) after treatment with BIX (20 mg/kg, once daily) and quantification of their expression in fold change relative to the control group. SOD activity (d), MDA content (e), and H2O2 production (f) were also detected after treatment with BIX. The mRNA levels of proangiogenic factors were also determined by quantitative real-time PCR (g-l). Data are expressed as means ± SD ( n = 5). ∗ P

Techniques Used: Western Blot, Expressing, Activity Assay, Multiple Displacement Amplification, Real-time Polymerase Chain Reaction

Inhibition of G9a alleviated angiogenesis and oxidative stress through reduction of Nox4. Western blotting analysis for the expression of Nox4 and VEGF after transfection with an si-Nox4 (a-c). ROS production was measured after transfection with an si-Nox4 (d). H 2 O 2 production was measured after transfection with an si-Nox4 (e). The expression of Nox4 and VEGF was evaluated through western blot after treatment with BIX (10 μ M, application 2 h prior to H/R) or transfection with siRNA against G9a (f-h). ROS production was measured after treatment with BIX or transfection with siRNA against G9a (i). H 2 O 2 production was measured after transfection with an si-G9a (j). HUVECs were treated with 10 μ M BIX for 2 h and then infected with an adenovirus carrying the human Nox4 for 48 h, prior to exposure to the H/R process. Western blotting analysis for the expression of Nox4 and VEGF in different groups (k-m). (l,m) The production of ROS (n) and H 2 O 2 (o) was measured in different groups. Data are expressed as means ± SD ( n = 5). ∗ P
Figure Legend Snippet: Inhibition of G9a alleviated angiogenesis and oxidative stress through reduction of Nox4. Western blotting analysis for the expression of Nox4 and VEGF after transfection with an si-Nox4 (a-c). ROS production was measured after transfection with an si-Nox4 (d). H 2 O 2 production was measured after transfection with an si-Nox4 (e). The expression of Nox4 and VEGF was evaluated through western blot after treatment with BIX (10 μ M, application 2 h prior to H/R) or transfection with siRNA against G9a (f-h). ROS production was measured after treatment with BIX or transfection with siRNA against G9a (i). H 2 O 2 production was measured after transfection with an si-G9a (j). HUVECs were treated with 10 μ M BIX for 2 h and then infected with an adenovirus carrying the human Nox4 for 48 h, prior to exposure to the H/R process. Western blotting analysis for the expression of Nox4 and VEGF in different groups (k-m). (l,m) The production of ROS (n) and H 2 O 2 (o) was measured in different groups. Data are expressed as means ± SD ( n = 5). ∗ P

Techniques Used: Inhibition, Western Blot, Expressing, Transfection, Infection

8) Product Images from "Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation"

Article Title: Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation

Journal: Antioxidants

doi: 10.3390/antiox9100912

Effects of enzyme-treated Z. latifolia extract (ZLE) and tricin on the secretion of MMP-2, MMP-3, VEGF and cathepsin B in serum by UVA irradiation. ( A ) serum MMP-2; ( B ) serum MMP-3; ( C ) serum VEGF; ( D ) serum cathepsin B. The serum levels of the proteins were measured by ELISA. Data are expressed as the mean ± SE. The p values are determined by ANOVA and Tukey’s HSD test.
Figure Legend Snippet: Effects of enzyme-treated Z. latifolia extract (ZLE) and tricin on the secretion of MMP-2, MMP-3, VEGF and cathepsin B in serum by UVA irradiation. ( A ) serum MMP-2; ( B ) serum MMP-3; ( C ) serum VEGF; ( D ) serum cathepsin B. The serum levels of the proteins were measured by ELISA. Data are expressed as the mean ± SE. The p values are determined by ANOVA and Tukey’s HSD test.

Techniques Used: Irradiation, Enzyme-linked Immunosorbent Assay

9) Product Images from "Haemoptysis as a prognostic factor in lung adenocarcinoma after curative resection"

Article Title: Haemoptysis as a prognostic factor in lung adenocarcinoma after curative resection

Journal: British Journal of Cancer

doi: 10.1038/bjc.2013.485

Representative images of immunohistochemical staining. ( A ) Immunohistochemical staining of VEGF ( × 200). ( B ) Blood vessel invasion staining with CD34 ( × 400). The alphabet ‘a' indicates tumour cells in the vascular lumen, and the alphabet ‘b' indicates blood vessel, identified by CD34 staining positive. ( C ) Immunohistochemical staining of extratumoural MVD ( × 200). ( D ) Tumour necrosis ( × 100).
Figure Legend Snippet: Representative images of immunohistochemical staining. ( A ) Immunohistochemical staining of VEGF ( × 200). ( B ) Blood vessel invasion staining with CD34 ( × 400). The alphabet ‘a' indicates tumour cells in the vascular lumen, and the alphabet ‘b' indicates blood vessel, identified by CD34 staining positive. ( C ) Immunohistochemical staining of extratumoural MVD ( × 200). ( D ) Tumour necrosis ( × 100).

Techniques Used: Immunohistochemistry, Staining

10) Product Images from "Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction"

Article Title: Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction

Journal: Stem Cells International

doi: 10.1155/2015/176409

Upregulation of angiogenic cytokines in hBcl-xL-MSCs. ((a)–(c)) Evaluation of the paracrine secretion of VEGF (a), IGF-1 (b), and PDGF (c) by wild type MSCs (pale grey bar), vector-MSCs (dark grey bar), and hBcl-xL-MSCs (black bar). Cells were cultured under either normoxic or hypoxic conditions for 24 hours and the conditioned medium was collected for ELISA. Concentration values are mean ± SD ( n = 3, ∗ P
Figure Legend Snippet: Upregulation of angiogenic cytokines in hBcl-xL-MSCs. ((a)–(c)) Evaluation of the paracrine secretion of VEGF (a), IGF-1 (b), and PDGF (c) by wild type MSCs (pale grey bar), vector-MSCs (dark grey bar), and hBcl-xL-MSCs (black bar). Cells were cultured under either normoxic or hypoxic conditions for 24 hours and the conditioned medium was collected for ELISA. Concentration values are mean ± SD ( n = 3, ∗ P

Techniques Used: Plasmid Preparation, Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay

11) Product Images from "Detection of Tumor Associated Neoangiogenesis by Doppler Ultrasound During Early-Stage Ovarian Cancer in Laying Hens: A Preclinical Model of Human Spontaneous Ovarian Cancer"

Article Title: Detection of Tumor Associated Neoangiogenesis by Doppler Ultrasound During Early-Stage Ovarian Cancer in Laying Hens: A Preclinical Model of Human Spontaneous Ovarian Cancer

Journal: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

doi:

Expression of neoangiogenic markers in hen ovaries predicted to be normal and with ovarian TAN by ultrasonography. A , Section showing normal vasculature with few VEGF stained vessels in the follicular theca and stroma of a hen monitored prospectively
Figure Legend Snippet: Expression of neoangiogenic markers in hen ovaries predicted to be normal and with ovarian TAN by ultrasonography. A , Section showing normal vasculature with few VEGF stained vessels in the follicular theca and stroma of a hen monitored prospectively

Techniques Used: Expressing, Staining

12) Product Images from "eIF2α signaling regulates ischemic osteonecrosis through endoplasmic reticulum stress"

Article Title: eIF2α signaling regulates ischemic osteonecrosis through endoplasmic reticulum stress

Journal: Scientific Reports

doi: 10.1038/s41598-017-05488-6

Salubrinal increases p-eIF2α, ATF4, GRP78, CHOP and VEGF, and reduces NFATc1 in ONFH in vivo . ( a ) Representative histological immunofluorescence images of femoral head from different groups (blue: DAPI, green: p-eIF2α + cells, 200×, Bar = 100 μm). ( b ) Numbers of p-eIF2α + cells were analyzed. ( c ) Representative images of Western blotting in three different groups in vivo . The levels of p-eIF2α ( d ), ATF4 ( e ), GRP78 ( f ), CHOP ( g ), VEGF ( h ), and NFATc1 ( i ) are shown. The asterisks (* and **) represent P
Figure Legend Snippet: Salubrinal increases p-eIF2α, ATF4, GRP78, CHOP and VEGF, and reduces NFATc1 in ONFH in vivo . ( a ) Representative histological immunofluorescence images of femoral head from different groups (blue: DAPI, green: p-eIF2α + cells, 200×, Bar = 100 μm). ( b ) Numbers of p-eIF2α + cells were analyzed. ( c ) Representative images of Western blotting in three different groups in vivo . The levels of p-eIF2α ( d ), ATF4 ( e ), GRP78 ( f ), CHOP ( g ), VEGF ( h ), and NFATc1 ( i ) are shown. The asterisks (* and **) represent P

Techniques Used: In Vivo, Immunofluorescence, Western Blot

13) Product Images from "Re-expression of DIRAS3 and p53 induces apoptosis and impaired autophagy in head and neck squamous cell carcinoma"

Article Title: Re-expression of DIRAS3 and p53 induces apoptosis and impaired autophagy in head and neck squamous cell carcinoma

Journal: Military Medical Research

doi: 10.1186/s40779-020-00275-3

DIRAS3 and p53 re-expression inhibits the expression of multiple oncogenes in HNSCC cells. a. CAL-27 cells were treated with Ad-DIRAS3, rAd-p53, and their combination for 24 h. Ad-GFP was used as a negative control. The expression of c-Myc, cyclin D1, VEGF, FGF, EGFR and Bcl-2 proteins was determined by western blotting. b. Relative mRNA levels of c-Myc, cyclin D1, VEGF, FGF, EGFR and Bcl-2 were examined by real-time PCR. ** P
Figure Legend Snippet: DIRAS3 and p53 re-expression inhibits the expression of multiple oncogenes in HNSCC cells. a. CAL-27 cells were treated with Ad-DIRAS3, rAd-p53, and their combination for 24 h. Ad-GFP was used as a negative control. The expression of c-Myc, cyclin D1, VEGF, FGF, EGFR and Bcl-2 proteins was determined by western blotting. b. Relative mRNA levels of c-Myc, cyclin D1, VEGF, FGF, EGFR and Bcl-2 were examined by real-time PCR. ** P

Techniques Used: Expressing, Negative Control, Western Blot, Real-time Polymerase Chain Reaction

14) Product Images from "Restored microRNA-133a-3p or Depleted PSAT1 Restrains Endothelial Cell Damage-Induced Intracranial Aneurysm Via Suppressing the GSK3β/β-Catenin Pathway"

Article Title: Restored microRNA-133a-3p or Depleted PSAT1 Restrains Endothelial Cell Damage-Induced Intracranial Aneurysm Via Suppressing the GSK3β/β-Catenin Pathway

Journal: Nanoscale Research Letters

doi: 10.1186/s11671-020-03396-9

Overexpression of miR-133a-3p and low expression of PSAT1 decrease PSAT1, GSK3β, and β-catenin, VEGF and MMP-9 expression in IA tissues in vivo and PSAT1 is a target gene of miR-133a-3p. a Detection of miR-133a-3p, PSAT1, GSK3β, and β-catenin expression in IA tissues of rats in each group by RT-qPCR. b Protein bands of PSAT1, GSK3β, and β-catenin. c Detection of PSAT1, GSK3β, and β-catenin protein expression in IA tissues of rats in each group by western blot analysis. d Protein bands of VEGF and MMP-9. e Detection of VEGF and MMP-9 protein expression in IA tissues of rats in each group by western blot analysis. f Prediction of the target site of PSAT1 binding to the corresponding miR-133a-3p by Target Scan. g Result of dual luciferase reporter gene assay. a – e , n = 12; f – g , N = 3, * P
Figure Legend Snippet: Overexpression of miR-133a-3p and low expression of PSAT1 decrease PSAT1, GSK3β, and β-catenin, VEGF and MMP-9 expression in IA tissues in vivo and PSAT1 is a target gene of miR-133a-3p. a Detection of miR-133a-3p, PSAT1, GSK3β, and β-catenin expression in IA tissues of rats in each group by RT-qPCR. b Protein bands of PSAT1, GSK3β, and β-catenin. c Detection of PSAT1, GSK3β, and β-catenin protein expression in IA tissues of rats in each group by western blot analysis. d Protein bands of VEGF and MMP-9. e Detection of VEGF and MMP-9 protein expression in IA tissues of rats in each group by western blot analysis. f Prediction of the target site of PSAT1 binding to the corresponding miR-133a-3p by Target Scan. g Result of dual luciferase reporter gene assay. a – e , n = 12; f – g , N = 3, * P

Techniques Used: Over Expression, Expressing, In Vivo, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Reporter Gene Assay

Pathological changes of aneurysm and the expression of MMP-9 and VEGF in IA. a Normal intracranial arterioles tissue sections in the control group under HE staining (× 10). b IA tissue sections dyed with HE (× 10). c Ultrastructure of normal intracranial arterioles tissues in the control group under an electron microscope (× 10,000). d Ultrastructure of IA tissues under an electron microscope (× 10,000). e Expression of MMP-9 in the control group and the IA group by immunohistochemical staining (× 200). f Expression of VEGF in the control group and the IA group by immunohistochemical staining (× 200)
Figure Legend Snippet: Pathological changes of aneurysm and the expression of MMP-9 and VEGF in IA. a Normal intracranial arterioles tissue sections in the control group under HE staining (× 10). b IA tissue sections dyed with HE (× 10). c Ultrastructure of normal intracranial arterioles tissues in the control group under an electron microscope (× 10,000). d Ultrastructure of IA tissues under an electron microscope (× 10,000). e Expression of MMP-9 in the control group and the IA group by immunohistochemical staining (× 200). f Expression of VEGF in the control group and the IA group by immunohistochemical staining (× 200)

Techniques Used: Expressing, Staining, Microscopy, Immunohistochemistry

15) Product Images from "Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells"

Article Title: Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells

Journal: BMC Complementary Medicine and Therapies

doi: 10.1186/s12906-020-03189-8

Norwogonin down-regulated the expression of HIF-1 α and VEGF in PC12 cells following hypoxia exposure. Cells were incubated with norwogonin for 1 h and then exposed to hypoxia for 24 h. The mRNA expression was determined by the real-time qPCR ( a ). The expressions of HIF-1α and VEGF proteins were detected using Western blot analysis ( b ). In order to improve the clarity and conciseness of the presentation, the blots were cropped. Statistical analysis results of HIF-1α ( c ) and VEGF ( d ) from Western blot analysis. Results are expressed as means ± SD, n = 3. ## P
Figure Legend Snippet: Norwogonin down-regulated the expression of HIF-1 α and VEGF in PC12 cells following hypoxia exposure. Cells were incubated with norwogonin for 1 h and then exposed to hypoxia for 24 h. The mRNA expression was determined by the real-time qPCR ( a ). The expressions of HIF-1α and VEGF proteins were detected using Western blot analysis ( b ). In order to improve the clarity and conciseness of the presentation, the blots were cropped. Statistical analysis results of HIF-1α ( c ) and VEGF ( d ) from Western blot analysis. Results are expressed as means ± SD, n = 3. ## P

Techniques Used: Expressing, Incubation, Real-time Polymerase Chain Reaction, Western Blot

16) Product Images from "Anticancer Effects of Baicalein in Pancreatic Neuroendocrine Tumors In Vitro and In Vivo"

Article Title: Anticancer Effects of Baicalein in Pancreatic Neuroendocrine Tumors In Vitro and In Vivo

Journal: Pancreas

doi: 10.1097/MPA.0000000000000895

Baicalein inhibited BON1 migration and invasion. A, Migration activity of BON1 treated with 100 μM baicalein for 24 and 48 hours was measured by Transwell assay. Cell migration number is presented. B, The protein levels of Bax, Bcl-2, VEGF, MMP-2, and MMP-9 were analyzed by Western blot, and quantification assay of Western blot results is also presented. * P
Figure Legend Snippet: Baicalein inhibited BON1 migration and invasion. A, Migration activity of BON1 treated with 100 μM baicalein for 24 and 48 hours was measured by Transwell assay. Cell migration number is presented. B, The protein levels of Bax, Bcl-2, VEGF, MMP-2, and MMP-9 were analyzed by Western blot, and quantification assay of Western blot results is also presented. * P

Techniques Used: Migration, Activity Assay, Transwell Assay, Western Blot

Baicalein abolished tumorigenesis of pNETs in mice. A, BON1 xenograft tumors from the treatment experiment. Mice were killed 7 weeks after baicalein treatment. B, The tumor volumes were calculated according to the following formula: volume = length × width 2 / 2. C, The protein obtained from primary tumors in mice with or without baicalein treatment. The levels of Bax, Bcl-2, VEGF, survivin, MMP-2, and MMP-9 were analyzed by Western blot, and quantification assay of Western blot results is also presented. * P
Figure Legend Snippet: Baicalein abolished tumorigenesis of pNETs in mice. A, BON1 xenograft tumors from the treatment experiment. Mice were killed 7 weeks after baicalein treatment. B, The tumor volumes were calculated according to the following formula: volume = length × width 2 / 2. C, The protein obtained from primary tumors in mice with or without baicalein treatment. The levels of Bax, Bcl-2, VEGF, survivin, MMP-2, and MMP-9 were analyzed by Western blot, and quantification assay of Western blot results is also presented. * P

Techniques Used: Mouse Assay, Western Blot

17) Product Images from "MiR-511 mimic transfection inhibits the proliferation, invasion of osteosarcoma cells and reduces metastatic osteosarcoma tumor burden in nude mice via targeting MAPK1"

Article Title: MiR-511 mimic transfection inhibits the proliferation, invasion of osteosarcoma cells and reduces metastatic osteosarcoma tumor burden in nude mice via targeting MAPK1

Journal: Cancer Biomarkers

doi: 10.3233/CBM-190534

Up-regulated miR-511 suppressed the progression of osteosarcoma in vivo . Nude mice were randomly divided into 2 groups. Control group: tumor extracted from nude mice receiving subcutaneous injection of MG63 cells transfected with miR-NC; mimic group: tumor extracted from nude mice receiving subcutaneous injection of MG63 cells transfected with miR-511 mimic. (A) Gross tumor weight was detected. (B) The representative tumor images were exhibited. (C) The expressions of MAPK1 and miR-511 were evaluated using RT-PCR. (D) The levels of Ki67 and VEGF were detected by immunohistochemical assay. (E) The tumor focal lesions in liver/lung were detected by HE staining. The representative tumor images were exhibited. The experiments were repeated at least 3 times, and error bars represent ± SD ( P ∗
Figure Legend Snippet: Up-regulated miR-511 suppressed the progression of osteosarcoma in vivo . Nude mice were randomly divided into 2 groups. Control group: tumor extracted from nude mice receiving subcutaneous injection of MG63 cells transfected with miR-NC; mimic group: tumor extracted from nude mice receiving subcutaneous injection of MG63 cells transfected with miR-511 mimic. (A) Gross tumor weight was detected. (B) The representative tumor images were exhibited. (C) The expressions of MAPK1 and miR-511 were evaluated using RT-PCR. (D) The levels of Ki67 and VEGF were detected by immunohistochemical assay. (E) The tumor focal lesions in liver/lung were detected by HE staining. The representative tumor images were exhibited. The experiments were repeated at least 3 times, and error bars represent ± SD ( P ∗

Techniques Used: In Vivo, Mouse Assay, Injection, Transfection, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Staining

18) Product Images from "Salvianolic Acid A Inhibits OX-LDL Effects on Exacerbating Choroidal Neovascularization via Downregulating CYLD"

Article Title: Salvianolic Acid A Inhibits OX-LDL Effects on Exacerbating Choroidal Neovascularization via Downregulating CYLD

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2017/6210694

Real-time PCR analysis of VEGE, PDGF, PEDF, and angiostatin mRNA expression in four groups 7 days after laser. (a, b) OX-LDL injection increased both VEGF and PDGF mRNA expression compared with the PBS group, while OX-LDL + Sal A group
Figure Legend Snippet: Real-time PCR analysis of VEGE, PDGF, PEDF, and angiostatin mRNA expression in four groups 7 days after laser. (a, b) OX-LDL injection increased both VEGF and PDGF mRNA expression compared with the PBS group, while OX-LDL + Sal A group

Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Injection

19) Product Images from "Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway"

Article Title: Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway

Journal: International Journal of Biological Sciences

doi: 10.7150/ijbs.10785

Effect of GO and NDEA on the protein expressions of COX-2, iNOS and VEGF. A: A representative immunoblot. B: Data presented the expressions of COX-2, iNOS and VEGF as percentage of control group (mean ± SD) in triplicate. The protein levels were quantified with β-actin as an internal control. * P
Figure Legend Snippet: Effect of GO and NDEA on the protein expressions of COX-2, iNOS and VEGF. A: A representative immunoblot. B: Data presented the expressions of COX-2, iNOS and VEGF as percentage of control group (mean ± SD) in triplicate. The protein levels were quantified with β-actin as an internal control. * P

Techniques Used:

20) Product Images from "Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell Metastasis Through Activating MAPK Signaling Pathway on HUVECs"

Article Title: Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell Metastasis Through Activating MAPK Signaling Pathway on HUVECs

Journal: Technology in Cancer Research & Treatment

doi: 10.1177/1533033818785512

The expression of p38, p-p38, ERK, p-ERK,JNK, p-JNK and VEGF. a: control, b:single AE group, c:single light group, d: AE-PDT group.
Figure Legend Snippet: The expression of p38, p-p38, ERK, p-ERK,JNK, p-JNK and VEGF. a: control, b:single AE group, c:single light group, d: AE-PDT group.

Techniques Used: Expressing

21) Product Images from "Cigarette smoke inhalation impairs angiogenesis in early bone healing processes and delays fracture union"

Article Title: Cigarette smoke inhalation impairs angiogenesis in early bone healing processes and delays fracture union

Journal: Bone & Joint Research

doi: 10.1302/2046-3758.93.BJR-2019-0089.R1

Cigarette smoke inhalation reduced the expression of vascular endothelial growth factor (VEGF) and von Willebrand factor (vWF) protein in the fracture callus. a) Western blot analysis was used to detect the expression of VEGF and vWF protein in the fracture callus. β-actin was used as an internal control (n = 4). One representative data set obtained from repeated experiments is shown. b) Quantified data of Western blot analysis for the expression of VEGF in the smoking group, two and four weeks after the fracture (n = 4). c) Quantified data of Western blot analysis for expression of vWF in the smoking group, two and four weeks after the fracture (n = 4). Data are shown as the mean (SD) of six experiments. *p
Figure Legend Snippet: Cigarette smoke inhalation reduced the expression of vascular endothelial growth factor (VEGF) and von Willebrand factor (vWF) protein in the fracture callus. a) Western blot analysis was used to detect the expression of VEGF and vWF protein in the fracture callus. β-actin was used as an internal control (n = 4). One representative data set obtained from repeated experiments is shown. b) Quantified data of Western blot analysis for the expression of VEGF in the smoking group, two and four weeks after the fracture (n = 4). c) Quantified data of Western blot analysis for expression of vWF in the smoking group, two and four weeks after the fracture (n = 4). Data are shown as the mean (SD) of six experiments. *p

Techniques Used: Expressing, Western Blot

22) Product Images from "An Immunocompetent, Orthotopic Mouse Model of Epithelial Ovarian Cancer Utilizing Tissue Engineered Tumor Cell Sheets"

Article Title: An Immunocompetent, Orthotopic Mouse Model of Epithelial Ovarian Cancer Utilizing Tissue Engineered Tumor Cell Sheets

Journal: Tissue Engineering. Part C, Methods

doi: 10.1089/ten.tec.2014.0040

(A) Vascular endothelial growth factor (VEGF) and CD31 immunostaining. (a) VEGF-positive margins were found surrounding CS-derived tumors at 1 week post-transplantation, ×40, scale bar is 200 μm and (b) was magnified from a small
Figure Legend Snippet: (A) Vascular endothelial growth factor (VEGF) and CD31 immunostaining. (a) VEGF-positive margins were found surrounding CS-derived tumors at 1 week post-transplantation, ×40, scale bar is 200 μm and (b) was magnified from a small

Techniques Used: Immunostaining, Derivative Assay, Transplantation Assay

23) Product Images from "Cochlear epithelial of dog fetuses: a new source of multipotent stem cells"

Article Title: Cochlear epithelial of dog fetuses: a new source of multipotent stem cells

Journal: Cytotechnology

doi: 10.1007/s10616-016-0049-0

Immunophenotyping by flow cytometry. Positive expression for cytoskeletal markers (cytokeratin and β-tubulin) and most mesenchymal cell markers (Stro-1, CD90 and CD105). High levels of expression for vascular endothelial growth factor marker (VEGF), hematopoietic precursor cell marker (CD117), and negative response for hematopoietic cells (CD34) and neural precursor cells (Nestin); expression of markers for ciliated cells (Myosin VIIa) and pluripotent cells (Sox-2 and Oct-4)
Figure Legend Snippet: Immunophenotyping by flow cytometry. Positive expression for cytoskeletal markers (cytokeratin and β-tubulin) and most mesenchymal cell markers (Stro-1, CD90 and CD105). High levels of expression for vascular endothelial growth factor marker (VEGF), hematopoietic precursor cell marker (CD117), and negative response for hematopoietic cells (CD34) and neural precursor cells (Nestin); expression of markers for ciliated cells (Myosin VIIa) and pluripotent cells (Sox-2 and Oct-4)

Techniques Used: Flow Cytometry, Cytometry, Expressing, Marker

24) Product Images from "Fluoxetine Induces Apoptosis through Extrinsic/Intrinsic Pathways and Inhibits ERK/NF-κB-Modulated Anti-Apoptotic and Invasive Potential in Hepatocellular Carcinoma Cells In Vitro"

Article Title: Fluoxetine Induces Apoptosis through Extrinsic/Intrinsic Pathways and Inhibits ERK/NF-κB-Modulated Anti-Apoptotic and Invasive Potential in Hepatocellular Carcinoma Cells In Vitro

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms20030757

Fluoxetine decreased the cell migration/invasion and expression of pERK, metastasis-associated and proliferative proteins in SK-Hep1 and Hep3B cells. SK-Hep1 and Hep3B cells were treated with 0, 30 µM, and 40 µM of fluoxetine for 48 h. Then ( A , C ) migration assay or ( B , D ) invasion assay was performed. Quantification results were measured by four selected field per group. ( E ) Western blots were performed with P-ERK, T-ERK, MMP-9, VEGF and CyclinD1 antibodies to the indicated protein expression after fluoxetine treatment on SK-Hep1 cells. Quantification data were normalized by β-actin expression and averaged over three repeated experiments. ** p
Figure Legend Snippet: Fluoxetine decreased the cell migration/invasion and expression of pERK, metastasis-associated and proliferative proteins in SK-Hep1 and Hep3B cells. SK-Hep1 and Hep3B cells were treated with 0, 30 µM, and 40 µM of fluoxetine for 48 h. Then ( A , C ) migration assay or ( B , D ) invasion assay was performed. Quantification results were measured by four selected field per group. ( E ) Western blots were performed with P-ERK, T-ERK, MMP-9, VEGF and CyclinD1 antibodies to the indicated protein expression after fluoxetine treatment on SK-Hep1 cells. Quantification data were normalized by β-actin expression and averaged over three repeated experiments. ** p

Techniques Used: Migration, Expressing, Invasion Assay, Western Blot

25) Product Images from "Changes in Caspase-3, B Cell Leukemia/Lymphoma-2, Interleukin-6, Tumor Necrosis Factor-α and Vascular Endothelial Growth Factor Gene Expression after Human Umbilical Cord Blood Derived Mesenchymal Stem Cells Transfusion in Pulmonary Hypertension Rat Models"

Article Title: Changes in Caspase-3, B Cell Leukemia/Lymphoma-2, Interleukin-6, Tumor Necrosis Factor-α and Vascular Endothelial Growth Factor Gene Expression after Human Umbilical Cord Blood Derived Mesenchymal Stem Cells Transfusion in Pulmonary Hypertension Rat Models

Journal: Korean Circulation Journal

doi: 10.4070/kcj.2016.46.1.79

Changes of caspase-3 (A), Bcl-2 (B), VEGF (C), IL-6 (D), TNF-α (E), and BNP (F) protein expression levels after hUCB-MSCs injection in PAH rat heart tissues. The protein expressions of caspase-3, Bcl-2 and TNF-α were greatly decreased in the U group, as compared with the M group at week 2. The protein expressions of VEGF, IL-6 and BNP were significantly decreased in the U group in comparison with the M group at weeks 2 and 4 (p
Figure Legend Snippet: Changes of caspase-3 (A), Bcl-2 (B), VEGF (C), IL-6 (D), TNF-α (E), and BNP (F) protein expression levels after hUCB-MSCs injection in PAH rat heart tissues. The protein expressions of caspase-3, Bcl-2 and TNF-α were greatly decreased in the U group, as compared with the M group at week 2. The protein expressions of VEGF, IL-6 and BNP were significantly decreased in the U group in comparison with the M group at weeks 2 and 4 (p

Techniques Used: Expressing, Injection

Changes in caspase-3, Bcl-2, IL-6, TNF-α and VEGF protein expression levels after hUCB-MSCs injection in PAH rat lung tissues. The protein expression levels of caspase-3 (A), Bcl-2 (B), VEGF (C), IL-6 (D) and TNF-α (E) were significantly increased in the M group, as compared with the C group at weeks 2 and 4. The protein expression levels of caspase-3, Bcl-2, IL-6 and VEGF were greatly decreased in the M group, as compared with the C group at week 4. * p
Figure Legend Snippet: Changes in caspase-3, Bcl-2, IL-6, TNF-α and VEGF protein expression levels after hUCB-MSCs injection in PAH rat lung tissues. The protein expression levels of caspase-3 (A), Bcl-2 (B), VEGF (C), IL-6 (D) and TNF-α (E) were significantly increased in the M group, as compared with the C group at weeks 2 and 4. The protein expression levels of caspase-3, Bcl-2, IL-6 and VEGF were greatly decreased in the M group, as compared with the C group at week 4. * p

Techniques Used: Expressing, Injection

Immunohistochemical staing in the lung tissues. Immunohistochemical staining showed that caspase-3, Bcl-2, IL-6, TNF-α and VEGF expression levels were significantly higher in the M group than in the C group; however, the e xpression was lower in the U group than in the M group. * p
Figure Legend Snippet: Immunohistochemical staing in the lung tissues. Immunohistochemical staining showed that caspase-3, Bcl-2, IL-6, TNF-α and VEGF expression levels were significantly higher in the M group than in the C group; however, the e xpression was lower in the U group than in the M group. * p

Techniques Used: Immunohistochemistry, Staining, Expressing

Changes in caspase-3, Bcl-2, IL-6, TNF-α and VEGF mRNA expression levels after hUCB-MSCs injection in PAH rat lung tissues. The mRNA levels of caspase-3 (A), Bcl-2 (B), VEGF (C), IL-6 (D) and TNF-α (E) were significantly increased in the M group in comparison with the C group at weeks 2 and 4. The mRNA levels of caspase-3, Bcl-2, VEGF, IL-6 and TNF-α were greatly decreased in the U group, as compared with the M group at week 4. * p
Figure Legend Snippet: Changes in caspase-3, Bcl-2, IL-6, TNF-α and VEGF mRNA expression levels after hUCB-MSCs injection in PAH rat lung tissues. The mRNA levels of caspase-3 (A), Bcl-2 (B), VEGF (C), IL-6 (D) and TNF-α (E) were significantly increased in the M group in comparison with the C group at weeks 2 and 4. The mRNA levels of caspase-3, Bcl-2, VEGF, IL-6 and TNF-α were greatly decreased in the U group, as compared with the M group at week 4. * p

Techniques Used: Expressing, Injection

26) Product Images from "Transarterial oily chemoembolization with lidamycin shows potent therapeutic efficacy in VX2 rabbit liver tumor"

Article Title: Transarterial oily chemoembolization with lidamycin shows potent therapeutic efficacy in VX2 rabbit liver tumor

Journal: OncoTargets and therapy

doi: 10.2147/OTT.S89497

Immunohistochemistry analysis of VX2 tumor sections. Notes: Effects of ADM + IDO and LDM + IDO on tumor proliferation assessed via PCNA levels, tumor microvessel density assessed via CD31 levels, and tumor angiogenesis assessed via VEGF levels in paraffin-embedded VX2 tumor sections. The IHC analysis of PCNA, CD31, and VEGF expression indicated the enhanced inhibition of cell proliferation and angiogenesis in the LDM + IDO group compared to the ADM + IDO group. * P
Figure Legend Snippet: Immunohistochemistry analysis of VX2 tumor sections. Notes: Effects of ADM + IDO and LDM + IDO on tumor proliferation assessed via PCNA levels, tumor microvessel density assessed via CD31 levels, and tumor angiogenesis assessed via VEGF levels in paraffin-embedded VX2 tumor sections. The IHC analysis of PCNA, CD31, and VEGF expression indicated the enhanced inhibition of cell proliferation and angiogenesis in the LDM + IDO group compared to the ADM + IDO group. * P

Techniques Used: Immunohistochemistry, Expressing, Inhibition

27) Product Images from "Histological and Functional Benefit Following Transplantation of Motor Neuron Progenitors to the Injured Rat Spinal Cord"

Article Title: Histological and Functional Benefit Following Transplantation of Motor Neuron Progenitors to the Injured Rat Spinal Cord

Journal: PLoS ONE

doi: 10.1371/journal.pone.0011852

hMNPs secreted physiologically active growth factors. (a) Qualitative PCR analyses indicated that hMNPs express NT-3, NT-4, NGF, and VEGF. Neurite length was 58% longer (b) in cortical neuron cultures exposed to hMNP-CM for 7 days (c) as compared to cortical neuron cultures exposed to MN differentiation media (d). The neurofilament optical density was 45% greater (e) in the axonal chamber of microfluidic culture platforms in axotomized cortical neuron cultures exposed to hMNP-CM for 7 days (f) as compared to axotomized cortical neuron cultures exposed to MN differentiation media (g). (h) Neurite length was significantly attenuated in cortical neuron cultures exposed to hMNP-CM that contained function-blocking antibodies to MN growth factors. Immunofluorescent staining for MAP-2 in cultures exposed to control media (i) or hMNP-CM (j), in the presence of LPS. (k) Quantification of the number of MAP-2 positive neurons in the presence of control media or hMNP-CM, with and without LPS exposure. The number of neurons in cultures lacking LPS was not significantly different (p
Figure Legend Snippet: hMNPs secreted physiologically active growth factors. (a) Qualitative PCR analyses indicated that hMNPs express NT-3, NT-4, NGF, and VEGF. Neurite length was 58% longer (b) in cortical neuron cultures exposed to hMNP-CM for 7 days (c) as compared to cortical neuron cultures exposed to MN differentiation media (d). The neurofilament optical density was 45% greater (e) in the axonal chamber of microfluidic culture platforms in axotomized cortical neuron cultures exposed to hMNP-CM for 7 days (f) as compared to axotomized cortical neuron cultures exposed to MN differentiation media (g). (h) Neurite length was significantly attenuated in cortical neuron cultures exposed to hMNP-CM that contained function-blocking antibodies to MN growth factors. Immunofluorescent staining for MAP-2 in cultures exposed to control media (i) or hMNP-CM (j), in the presence of LPS. (k) Quantification of the number of MAP-2 positive neurons in the presence of control media or hMNP-CM, with and without LPS exposure. The number of neurons in cultures lacking LPS was not significantly different (p

Techniques Used: Polymerase Chain Reaction, Blocking Assay, Staining

28) Product Images from "Granule exocytosis is required for platelet spreading: differential sorting of ?-granules expressing VAMP-7"

Article Title: Granule exocytosis is required for platelet spreading: differential sorting of ?-granules expressing VAMP-7

Journal: Blood

doi: 10.1182/blood-2011-10-389247

Quantitative analysis of VAMP isoforms and cargo in spread platelets. Double immunofluorescence microscopy of actin and (A) VAMP-8, (B) serotonin, (C) VWF, (D) VAMP-7, (E) TIMP2, or (F) VEGF. Scale bars, 5 μm. (G) Images were evaluated as described in supplemental Figure 3 to demarcate the granulomere of the spread platelets and subsequently analyzed to quantify the amount of VAMP-8, serotonin, VWF, VAMP-7, TIMP2, and VEGF associated with the granulomere (black) versus the periphery (red). Error bars represent the standard deviation (SD) of measurements from 250 to 350 platelets per condition.
Figure Legend Snippet: Quantitative analysis of VAMP isoforms and cargo in spread platelets. Double immunofluorescence microscopy of actin and (A) VAMP-8, (B) serotonin, (C) VWF, (D) VAMP-7, (E) TIMP2, or (F) VEGF. Scale bars, 5 μm. (G) Images were evaluated as described in supplemental Figure 3 to demarcate the granulomere of the spread platelets and subsequently analyzed to quantify the amount of VAMP-8, serotonin, VWF, VAMP-7, TIMP2, and VEGF associated with the granulomere (black) versus the periphery (red). Error bars represent the standard deviation (SD) of measurements from 250 to 350 platelets per condition.

Techniques Used: Immunofluorescence, Microscopy, Standard Deviation

Colocalization studies demonstrate association of TIMP2, VEGF, and P-selectin with VAMP-7–expressing α-granules. Double fluorescence confocal microscopy of granules in spread platelets stained using antibodies to (A) anti-TIMP2, (B) anti-VEGF, or (C) P-selectin and counterstaining with anti–VAMP-7 antibody demonstrates peripheral colocalization of VAMP-7 with these α-granule markers. Scale bars, 5 μm.
Figure Legend Snippet: Colocalization studies demonstrate association of TIMP2, VEGF, and P-selectin with VAMP-7–expressing α-granules. Double fluorescence confocal microscopy of granules in spread platelets stained using antibodies to (A) anti-TIMP2, (B) anti-VEGF, or (C) P-selectin and counterstaining with anti–VAMP-7 antibody demonstrates peripheral colocalization of VAMP-7 with these α-granule markers. Scale bars, 5 μm.

Techniques Used: Expressing, Fluorescence, Confocal Microscopy, Staining

29) Product Images from "Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell Metastasis Through Activating MAPK Signaling Pathway on HUVECs"

Article Title: Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell Metastasis Through Activating MAPK Signaling Pathway on HUVECs

Journal: Technology in Cancer Research & Treatment

doi: 10.1177/1533033818785512

The expression of p38, p-p38, ERK, p-ERK,JNK, p-JNK and VEGF. a: control, b:single AE group, c:single light group, d: AE-PDT group.
Figure Legend Snippet: The expression of p38, p-p38, ERK, p-ERK,JNK, p-JNK and VEGF. a: control, b:single AE group, c:single light group, d: AE-PDT group.

Techniques Used: Expressing

30) Product Images from "Prolactin selectively transported to cerebrospinal fluid from blood under hypoxic/ischemic conditions"

Article Title: Prolactin selectively transported to cerebrospinal fluid from blood under hypoxic/ischemic conditions

Journal: PLoS ONE

doi: 10.1371/journal.pone.0198673

Amounts of PRL and VEGF in conditioned medium of SDR-P-1D5 and MSH-P3 cultured cells under hypoxic conditions. PRL levels under hypoxic conditions in (a) SDR-P-1D5 and (b) MSH-P3 cells. PRL were found to be at high levels in SDR-P-1D5 and MSH-P3 cells after 10 min incubation under 3% hypoxic conditions. However, PRL levels decreased after 20 min under hypoxic conditions. Measurement of vascular endothelial growth factor (VEGF) to confirm cell activity under hypoxic conditions in (c) SDR-P-1D5 and (d) MSH-P3 cells. Cell activity decreases following incubation for more than 20 min under hypoxic conditions. These experiments were performed five times.
Figure Legend Snippet: Amounts of PRL and VEGF in conditioned medium of SDR-P-1D5 and MSH-P3 cultured cells under hypoxic conditions. PRL levels under hypoxic conditions in (a) SDR-P-1D5 and (b) MSH-P3 cells. PRL were found to be at high levels in SDR-P-1D5 and MSH-P3 cells after 10 min incubation under 3% hypoxic conditions. However, PRL levels decreased after 20 min under hypoxic conditions. Measurement of vascular endothelial growth factor (VEGF) to confirm cell activity under hypoxic conditions in (c) SDR-P-1D5 and (d) MSH-P3 cells. Cell activity decreases following incubation for more than 20 min under hypoxic conditions. These experiments were performed five times.

Techniques Used: Cell Culture, Incubation, Activity Assay

31) Product Images from "Progesterone is neuroprotective against ischemic brain injury through its effects on the PI3K/Akt signaling pathway"

Article Title: Progesterone is neuroprotective against ischemic brain injury through its effects on the PI3K/Akt signaling pathway

Journal: Neuroscience

doi: 10.1016/j.neuroscience.2012.03.008

Effect of PROG on VEGF and BDNF expression at 24h, 72h and 14 days post-pMCAO. A. Western blot analysis of VEGF and BDNF expression from representative S, L and LP rats. B. Bar graph shows quantitative data for VEGF and BDNF from each group. Values are
Figure Legend Snippet: Effect of PROG on VEGF and BDNF expression at 24h, 72h and 14 days post-pMCAO. A. Western blot analysis of VEGF and BDNF expression from representative S, L and LP rats. B. Bar graph shows quantitative data for VEGF and BDNF from each group. Values are

Techniques Used: Expressing, Western Blot

32) Product Images from "Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects"

Article Title: Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects

Journal: PLoS ONE

doi: 10.1371/journal.pone.0131057

In vitro production of renotropic growth factors by fetal kidney cells. The fetal kidney cells significantly produced renotropic growth factors viz. VEGF, IGF-1, BMP-7 and bFGF in their culture supernatant (CS) as compared to fresh culture medium which served as control medium (CM). Values expressed as Mean±SEM. **p
Figure Legend Snippet: In vitro production of renotropic growth factors by fetal kidney cells. The fetal kidney cells significantly produced renotropic growth factors viz. VEGF, IGF-1, BMP-7 and bFGF in their culture supernatant (CS) as compared to fresh culture medium which served as control medium (CM). Values expressed as Mean±SEM. **p

Techniques Used: In Vitro, Produced

Effects of fetal kidney cells therapy on expression of growth factors and pro- and anti-inflammatory cytokines in rat kidney. (A-I) mRNA expression levels of growth factors viz. bFGF (A), BMP-7 (B), VEGF-A (C) and IGF-1(D), inflammatory cytokines viz. IL-1β (E), TNF-α (F), IFN-γ (G) and IL-6 (H), anti-inflammatory cytokine IL-10 (I). (J) Representative immunoblots showing the expression levels of inflammatory markers viz. NF-kB and ICAM-1 in the kidney tissues of sham operated, saline treated and fetal kidney cells treated groups. (K-L) Bar diagrams showing semi quantitative densitometry of the expression of NFκB and ICAM-1. Comparative gene expression ratio alculated by referring each gene to β-actin as an internal control. Densitometric analysis applied for comparison of relative protein expression and represented in densitometric arbitrary units (a. u.). Values expressed Mean±SEM. *p
Figure Legend Snippet: Effects of fetal kidney cells therapy on expression of growth factors and pro- and anti-inflammatory cytokines in rat kidney. (A-I) mRNA expression levels of growth factors viz. bFGF (A), BMP-7 (B), VEGF-A (C) and IGF-1(D), inflammatory cytokines viz. IL-1β (E), TNF-α (F), IFN-γ (G) and IL-6 (H), anti-inflammatory cytokine IL-10 (I). (J) Representative immunoblots showing the expression levels of inflammatory markers viz. NF-kB and ICAM-1 in the kidney tissues of sham operated, saline treated and fetal kidney cells treated groups. (K-L) Bar diagrams showing semi quantitative densitometry of the expression of NFκB and ICAM-1. Comparative gene expression ratio alculated by referring each gene to β-actin as an internal control. Densitometric analysis applied for comparison of relative protein expression and represented in densitometric arbitrary units (a. u.). Values expressed Mean±SEM. *p

Techniques Used: Expressing, Western Blot

33) Product Images from "The Therapeutic Value of Bone Marrow-Derived Endothelial Progenitor Cell Transplantation after Intracerebral Hemorrhage in Rats"

Article Title: The Therapeutic Value of Bone Marrow-Derived Endothelial Progenitor Cell Transplantation after Intracerebral Hemorrhage in Rats

Journal: Frontiers in Neurology

doi: 10.3389/fneur.2017.00174

Influence of endothelial progenitor cell (EPC) transplantation on protective cytokines . At 72 h after intracerebral hemorrhage, expression levels of protective cytokines, including VEGF, BDNF, NGF, and IGF-1, were measured in brain homogenates by Western blot analysis. Expression levels of VEGF, BDNF, and NGF increased in the EPC group compared with the phosphate-buffered saline group. n = 6 at each time point per group (* P
Figure Legend Snippet: Influence of endothelial progenitor cell (EPC) transplantation on protective cytokines . At 72 h after intracerebral hemorrhage, expression levels of protective cytokines, including VEGF, BDNF, NGF, and IGF-1, were measured in brain homogenates by Western blot analysis. Expression levels of VEGF, BDNF, and NGF increased in the EPC group compared with the phosphate-buffered saline group. n = 6 at each time point per group (* P

Techniques Used: Transplantation Assay, Expressing, Western Blot

34) Product Images from "Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period"

Article Title: Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period

Journal: Asian Spine Journal

doi: 10.4184/asj.2017.11.3.329

Detection of growth factors. (A) Expression levels of platelet-derived growth factor AB (PDGF AB), vascular endothelial growth factor receptor 2 (VEGF R2), transforming growth factor-β (TGF-β), and epidermal growth factor (EGF) were evaluated in fresh platelet-rich plasma (PRP) according to the intensity of the spots. The location of each is shown in the scheme. (B) PRP was stored at room tempertature for 8 weeks, and all growth factor expressions were almost undetectable. (C) Frozen PRP was stored for 8 weeks, after which faint expression of growth factors was observed. (D) The freeze-dried PRP was stored for 8 weeks, and almost all growth factors were expressed robustly.
Figure Legend Snippet: Detection of growth factors. (A) Expression levels of platelet-derived growth factor AB (PDGF AB), vascular endothelial growth factor receptor 2 (VEGF R2), transforming growth factor-β (TGF-β), and epidermal growth factor (EGF) were evaluated in fresh platelet-rich plasma (PRP) according to the intensity of the spots. The location of each is shown in the scheme. (B) PRP was stored at room tempertature for 8 weeks, and all growth factor expressions were almost undetectable. (C) Frozen PRP was stored for 8 weeks, after which faint expression of growth factors was observed. (D) The freeze-dried PRP was stored for 8 weeks, and almost all growth factors were expressed robustly.

Techniques Used: Expressing, Derivative Assay

Relative concentration of each growth factor (fold increase). Platelet-derived growth factor AB (PDGF AB) (A) , transforming growth factor-β (TGF-β) (B) , vascular endothelial growth factor receptor 2 (VEGF R2) (C) , and epidermal growth factor (EGF) levels (D) were assessed over time. In the room temperature (RT) group, there was a clear reduction and/or disappearance of the growth factor expression after 2 weeks. In the frozen group, growth factor expression levels were decreased at 8 weeks. In contrast, the freeze-dried group maintained its original expression levels for the 8-week duration of the study. NS, not significant
Figure Legend Snippet: Relative concentration of each growth factor (fold increase). Platelet-derived growth factor AB (PDGF AB) (A) , transforming growth factor-β (TGF-β) (B) , vascular endothelial growth factor receptor 2 (VEGF R2) (C) , and epidermal growth factor (EGF) levels (D) were assessed over time. In the room temperature (RT) group, there was a clear reduction and/or disappearance of the growth factor expression after 2 weeks. In the frozen group, growth factor expression levels were decreased at 8 weeks. In contrast, the freeze-dried group maintained its original expression levels for the 8-week duration of the study. NS, not significant

Techniques Used: Concentration Assay, Derivative Assay, Expressing

35) Product Images from "Extracellular Vesicles Derived From Human Adipose-Derived Stem Cell Prevent the Formation of Hypertrophic Scar in a Rabbit Model"

Article Title: Extracellular Vesicles Derived From Human Adipose-Derived Stem Cell Prevent the Formation of Hypertrophic Scar in a Rabbit Model

Journal: Annals of Plastic Surgery

doi: 10.1097/SAP.0000000000002357

Characterization of hASCs and hASC-EVs. A, The spindle-shaped, fibroblast-like morphology of cultured (P3) hASCs. Scale bar, 100 μm. B, Oil Red O staining for hASCs adipogenic differentiation. C, The expression of hASCs surface markers CD49d, CD34, CD45, CD90, CD105, and CD73 was measured by flow cytometry. Human adipose-derived stem cells were highly positive for CD105, CD90, CD73, and CD49d but negative for CD34 and CD45. D, The size and spheroid morphology of the hASC-EVs (pointed by yellow arrow) are shown under TEM (×25,000; 80 kV); scale bar, 200 nm. E, The particle size of the hASC-EVs was assessed by NanoSight, approximately 95% of the particles were between 50 and 200 nm in size, with an average value of 129.1 nm. F, The specific vesicle-associated markers CD63, TSG101, and Alix were mainly detected in hASC-EVs but not in the EV-free medium of hASCs by Western blot. On the contrary, vascular endothelial growth factor A, platelet derived growth factor B, and TGFβ1 were highly expressed in the EV-free medium of hASCs.
Figure Legend Snippet: Characterization of hASCs and hASC-EVs. A, The spindle-shaped, fibroblast-like morphology of cultured (P3) hASCs. Scale bar, 100 μm. B, Oil Red O staining for hASCs adipogenic differentiation. C, The expression of hASCs surface markers CD49d, CD34, CD45, CD90, CD105, and CD73 was measured by flow cytometry. Human adipose-derived stem cells were highly positive for CD105, CD90, CD73, and CD49d but negative for CD34 and CD45. D, The size and spheroid morphology of the hASC-EVs (pointed by yellow arrow) are shown under TEM (×25,000; 80 kV); scale bar, 200 nm. E, The particle size of the hASC-EVs was assessed by NanoSight, approximately 95% of the particles were between 50 and 200 nm in size, with an average value of 129.1 nm. F, The specific vesicle-associated markers CD63, TSG101, and Alix were mainly detected in hASC-EVs but not in the EV-free medium of hASCs by Western blot. On the contrary, vascular endothelial growth factor A, platelet derived growth factor B, and TGFβ1 were highly expressed in the EV-free medium of hASCs.

Techniques Used: Cell Culture, Staining, Expressing, Flow Cytometry, Derivative Assay, Transmission Electron Microscopy, Western Blot

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Incubation:

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Article Snippet: .. The membrane was subsequently blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight at 4°C with the primary antibodies VEGFA (1:1,000; ab1316, Abcam) and GAPDH (sc-47724; 1:3,000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA). .. Following incubation with horseradish peroxidase-conjugated anti-rabbit (A0208; 1:5,000; Beyotime Institute of Biotechnology) and anti-mouse (A0216; 1:5,000; Beyotime Institute of Biotechnology) secondary antibodies at room temperature for 45 min, antibody binding signals were detected using the enhanced chemiluminescence luminol reagent (PerkinElmer Inc., Waltham, MA, USA) and a Chemi-doc image analyzer (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

Article Title: Platelets are associated with xenograft tumor growth and the clinical malignancy of ovarian cancer through an angiogenesis-dependent mechanism
Article Snippet: .. The tissue sections were then incubated with rabbit anti-CD41 polyclonal antibody (1:100; ab63983), mouse anti-CD31 monoclonal antibody (1:100; ab24590) and/or mouse anti-VEGF monoclonal antibody (1:200; ab1316), which were all purchased from Abcam, overnight at 4°C. .. The tissue sections were then incubated with a secondary goat anti-rabbit fluorescein isothiocyanate (111-095-003; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) and goat anti-mouse Rhodamine (115-295-003; Jackson ImmunoResearch Laboratories, Inc.) at 37°C for 1 h. The nuclei were counterstained with diamidinophenylindole (Sigma-Aldrich, St. Louis, MO, USA) and fluorescent images were captured using microscopy (BX51; Olympus, Tokyo, Japan) and analyzed using Image-Pro Plus software for colocalization.

Binding Assay:

Article Title: G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors
Article Snippet: .. Membranes were blocked with 5% non-fat skim milk in TBS containing 0.1% Tween 20 for 2 h at room temperature and probed with the following primary antibodies overnight at 4°C: Anti-Gs (1:500; cat no. sc26766), anti-Gi2 (1:500; cat no. sc391), anti-Gi3 (1:500; cat no. sc262), anti-G-protein-coupled receptor kinase 2 (GRK2; 1:500; cat no. sc562), anti-GATA binding protein 4 (GATA4; 1:1,000; cat no. sc1237) and anti-GAPDH (1:2,000; cat no. sc166574), obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA); anti-VEGF-A (1:1,000; cat no. ab1316) and anti-phosphorylated (p)-GATA4 (1:500; cat no. ab5245) obtained from Abcam (Cambridge, MA, USA). .. Membranes were then incubated with goat anti-rabbit horseradish peroxidase-conjugated secondary antibodies (1:5,000; cat no. SC-2004; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 h at room temperature.

Immunohistochemistry:

Article Title: Overexpression of HOXC10 promotes angiogenesis in human glioma via interaction with PRMT5 and upregulation of VEGFA expression
Article Snippet: .. IHC was performed using anti-HOXC10 polyclonal antibody (Abcam, ab153904), anti-CD31 polyclonal antibody (Abcam, ab28364), and anti-VEGFA monoclonal antibody (Abcam, ab1316, [VG-1]). ..

Cell Culture:

Article Title: Cyclosporine A stimulated hair growth from mouse vibrissae follicles in an organ culture model
Article Snippet: .. Vibrissae follicles were cultured at 37°C and 5% CO2 for 10 d. The medium was changed every other d. For neutralizing antibody experiments, follicles were cultured together with CsA and anti-VEGF (0.2 g/mL, ab1316, Abcam Cambridge, MA, USA) or anti-HGFR antibodies (1.5 g/mL, ab10728, Abcam). .. The length of the hair shafts of each follicle was measured every other d. Follicles were harvested, which were then embedded in Tissue-Tek® (SAKURA, Zoeterwoude, Netherlands) and stored at -80°C, or frozen in liquid nitrogen for total RNA extraction.

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    Abcam vegf r2
    Proposed cascade of events following activation of 12/15-LOX by hyperglycemia. Lipid metabolites of 12/15-LOX (12- and 15-HETE) activate vascular NADPH oxidase leading to overproduction of reactive oxygen species (ROS). Generation of ROS suppresses the activity of protein tyrosine phosphatases with subsequent activation of <t>VEGF-R2</t> signal pathway and disruption of retinal endothelial cell barrier.
    Vegf R2, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti vegf a
    Conformer selectivity of the peptides for different oncogenic quadruplexes in MCF-7 cells. ( A ) pGL4.72[ hRlucCP ] vector having the inserts containing oncogenic promoter sequences ( c-MYC, BCL-2, KRAS and <t>VEGF-A</t> ) and upstream G-quadruplex forming elements ahead of the hRluc coding region. The promoter sequences are cloned into KpnI and HindIII restriction sites with or without the wild-type quadruplex scaffolds. hRluc, Renilla luciferase gene; hCL1 and hPEST , protein destabilizing sequences; oriC, origin of replication; AmpR, ampicillin resistance gene; SV40 (Simian virus 40 polyadenylation signal cassette), P1 and P2, promoter sequences; null, constructs having no quadruplex motif upstream the oncogene promoters; GQ, G-quadruplex forming motif. ( B ) Dual-luciferase assays. Evaluation of the promoter activity of different oncogenes (( B ) c-MYC , ( C ) BCL-2 , ( D ) KRAS and ( E ) VEGF-A ) of using the reporter plasmids with or without the wild-type quadruplex-forming sequences (Pu27-GQ, BCL-2-GQ, KRAS-GQ and VEGF-A-GQ) in MCF-7 cells. Relative promoter activity is determined by normalizing the Rluc/Fluc values to that of the cells transfected with P1-P2 promoter construct (GQ-null), having no quadruplex-forming motif. Error bars represent mean ± SE ( N = 5). Statistical differences in the luciferase activities compared to the respective GQ-null constructs of the target oncogenes used one-way ANOVA followed by Tukey–Kramer Test ( # P
    Anti Vegf A, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam vegf
    RT-qPCR and Western blot analysis demonstrated that MBNL1-AS1 reduced the expression of KCNMA1 and cGMP-PKG signaling pathway-related genes. a RT-qPCR measured the relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, <t>VASP,</t> <t>VEGF,</t> Bax, and Bcl-2 in skeletal muscle cells after transfection. b Protein bands measured by Western blot analysis. c Western blot analysis measured the relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, p-PKGII phosphorylated-PKGII, VASP vasodilator-stimulated phosphoprotein, p-VASP phosphorylated-VASP, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, si small interfering, GAPDH glyceraldehyde-3-phosphate dehydrogenase, NC negative control; * p
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    Proposed cascade of events following activation of 12/15-LOX by hyperglycemia. Lipid metabolites of 12/15-LOX (12- and 15-HETE) activate vascular NADPH oxidase leading to overproduction of reactive oxygen species (ROS). Generation of ROS suppresses the activity of protein tyrosine phosphatases with subsequent activation of VEGF-R2 signal pathway and disruption of retinal endothelial cell barrier.

    Journal: PLoS ONE

    Article Title: 12/15-Lipoxygenase-Derived Lipid Metabolites Induce Retinal Endothelial Cell Barrier Dysfunction: Contribution of NADPH Oxidase

    doi: 10.1371/journal.pone.0057254

    Figure Lengend Snippet: Proposed cascade of events following activation of 12/15-LOX by hyperglycemia. Lipid metabolites of 12/15-LOX (12- and 15-HETE) activate vascular NADPH oxidase leading to overproduction of reactive oxygen species (ROS). Generation of ROS suppresses the activity of protein tyrosine phosphatases with subsequent activation of VEGF-R2 signal pathway and disruption of retinal endothelial cell barrier.

    Article Snippet: In particular, NADPH oxidase has been shown to modulate VEGF signaling pathway in endothelial cells via activating VEGF-R2 , .

    Techniques: Activation Assay, Activity Assay

    12-HETE induces phosphorylation of VEGF-R2 and dephosphorylation of protein tyrosine phosphatase (PTP) SHP1 in REC. Western blotting analysis (A) demonstrated significant increase in the level of pVEGF-R2 after 5 and 60 minutes from the beginning of treatment with 12-HETE. *P

    Journal: PLoS ONE

    Article Title: 12/15-Lipoxygenase-Derived Lipid Metabolites Induce Retinal Endothelial Cell Barrier Dysfunction: Contribution of NADPH Oxidase

    doi: 10.1371/journal.pone.0057254

    Figure Lengend Snippet: 12-HETE induces phosphorylation of VEGF-R2 and dephosphorylation of protein tyrosine phosphatase (PTP) SHP1 in REC. Western blotting analysis (A) demonstrated significant increase in the level of pVEGF-R2 after 5 and 60 minutes from the beginning of treatment with 12-HETE. *P

    Article Snippet: In particular, NADPH oxidase has been shown to modulate VEGF signaling pathway in endothelial cells via activating VEGF-R2 , .

    Techniques: De-Phosphorylation Assay, Western Blot

    Effect of VEGF-R2 inhibition on 12-HETE-induced REC hyperpermeability. Retinal endothelial cells were treated with 0.1 µM 12-HETE in the presence or absence of the VEGF-R2 inhibitor, ZM323881 hydrochloride (10 nM) for 12 hrs before adding the FITC-dextran to the upper chamber of the transwell. Four hrs later the fluorescence intensity in the lower chamber was measured by the plate reader and corrected to the intensity in the upper one. The permeability effect of 12-HETE was significantly prevented by the ZM323881 hydrochloride (12-HETE+I). * P

    Journal: PLoS ONE

    Article Title: 12/15-Lipoxygenase-Derived Lipid Metabolites Induce Retinal Endothelial Cell Barrier Dysfunction: Contribution of NADPH Oxidase

    doi: 10.1371/journal.pone.0057254

    Figure Lengend Snippet: Effect of VEGF-R2 inhibition on 12-HETE-induced REC hyperpermeability. Retinal endothelial cells were treated with 0.1 µM 12-HETE in the presence or absence of the VEGF-R2 inhibitor, ZM323881 hydrochloride (10 nM) for 12 hrs before adding the FITC-dextran to the upper chamber of the transwell. Four hrs later the fluorescence intensity in the lower chamber was measured by the plate reader and corrected to the intensity in the upper one. The permeability effect of 12-HETE was significantly prevented by the ZM323881 hydrochloride (12-HETE+I). * P

    Article Snippet: In particular, NADPH oxidase has been shown to modulate VEGF signaling pathway in endothelial cells via activating VEGF-R2 , .

    Techniques: Inhibition, Fluorescence, Permeability

    Conformer selectivity of the peptides for different oncogenic quadruplexes in MCF-7 cells. ( A ) pGL4.72[ hRlucCP ] vector having the inserts containing oncogenic promoter sequences ( c-MYC, BCL-2, KRAS and VEGF-A ) and upstream G-quadruplex forming elements ahead of the hRluc coding region. The promoter sequences are cloned into KpnI and HindIII restriction sites with or without the wild-type quadruplex scaffolds. hRluc, Renilla luciferase gene; hCL1 and hPEST , protein destabilizing sequences; oriC, origin of replication; AmpR, ampicillin resistance gene; SV40 (Simian virus 40 polyadenylation signal cassette), P1 and P2, promoter sequences; null, constructs having no quadruplex motif upstream the oncogene promoters; GQ, G-quadruplex forming motif. ( B ) Dual-luciferase assays. Evaluation of the promoter activity of different oncogenes (( B ) c-MYC , ( C ) BCL-2 , ( D ) KRAS and ( E ) VEGF-A ) of using the reporter plasmids with or without the wild-type quadruplex-forming sequences (Pu27-GQ, BCL-2-GQ, KRAS-GQ and VEGF-A-GQ) in MCF-7 cells. Relative promoter activity is determined by normalizing the Rluc/Fluc values to that of the cells transfected with P1-P2 promoter construct (GQ-null), having no quadruplex-forming motif. Error bars represent mean ± SE ( N = 5). Statistical differences in the luciferase activities compared to the respective GQ-null constructs of the target oncogenes used one-way ANOVA followed by Tukey–Kramer Test ( # P

    Journal: Nucleic Acids Research

    Article Title: Site-specific amino acid substitution in dodecameric peptides determines the stability and unfolding of c-MYC quadruplex promoting apoptosis in cancer cells

    doi: 10.1093/nar/gky824

    Figure Lengend Snippet: Conformer selectivity of the peptides for different oncogenic quadruplexes in MCF-7 cells. ( A ) pGL4.72[ hRlucCP ] vector having the inserts containing oncogenic promoter sequences ( c-MYC, BCL-2, KRAS and VEGF-A ) and upstream G-quadruplex forming elements ahead of the hRluc coding region. The promoter sequences are cloned into KpnI and HindIII restriction sites with or without the wild-type quadruplex scaffolds. hRluc, Renilla luciferase gene; hCL1 and hPEST , protein destabilizing sequences; oriC, origin of replication; AmpR, ampicillin resistance gene; SV40 (Simian virus 40 polyadenylation signal cassette), P1 and P2, promoter sequences; null, constructs having no quadruplex motif upstream the oncogene promoters; GQ, G-quadruplex forming motif. ( B ) Dual-luciferase assays. Evaluation of the promoter activity of different oncogenes (( B ) c-MYC , ( C ) BCL-2 , ( D ) KRAS and ( E ) VEGF-A ) of using the reporter plasmids with or without the wild-type quadruplex-forming sequences (Pu27-GQ, BCL-2-GQ, KRAS-GQ and VEGF-A-GQ) in MCF-7 cells. Relative promoter activity is determined by normalizing the Rluc/Fluc values to that of the cells transfected with P1-P2 promoter construct (GQ-null), having no quadruplex-forming motif. Error bars represent mean ± SE ( N = 5). Statistical differences in the luciferase activities compared to the respective GQ-null constructs of the target oncogenes used one-way ANOVA followed by Tukey–Kramer Test ( # P

    Article Snippet: Santacruz Biotechnology Inc.), anti E2F1, anti VEGF-A, anti-P53, anti-APAF1, anti-Caspase 8, anti-PARP (Abcam), anti-β-actin (Mouse monoclonal, 1:10 000 dil.

    Techniques: Plasmid Preparation, Clone Assay, Luciferase, Construct, Activity Assay, Transfection

    Selective arresting of c-MYC quadruplex by KR12C induce apoptotic signalling in cancer cells. ( A ) Western blot analyses of the major apoptotic markers (BCL-2, APAF-1, caspase 8 and PARP). ( B ) Estimation of protein expression level of the apoptotic markers by semi-densitometric analyses. Estimation of BCL-2 transcripts by real time PCR analyses at 5 and 10 μM KR12C concentration. ( C ) Western blot analyses of other proteins associated with the signalling cascade (c-MYC, E2F-1, VEGF-A and P53). ( D ) Quatification of protein and mRNA expression level of c-MYC, E2F-1, VEGF-A and P53 by semi-densitometric analyses and real time PCR respectively. Arrow pointing upwards and downwards denote the upregulation of the respective protein and mRNA expression level. Error bars represent mean ± SE ( N = 3). Statistical differences are determined compared to the control by two-tailed Student's t test (* P

    Journal: Nucleic Acids Research

    Article Title: Site-specific amino acid substitution in dodecameric peptides determines the stability and unfolding of c-MYC quadruplex promoting apoptosis in cancer cells

    doi: 10.1093/nar/gky824

    Figure Lengend Snippet: Selective arresting of c-MYC quadruplex by KR12C induce apoptotic signalling in cancer cells. ( A ) Western blot analyses of the major apoptotic markers (BCL-2, APAF-1, caspase 8 and PARP). ( B ) Estimation of protein expression level of the apoptotic markers by semi-densitometric analyses. Estimation of BCL-2 transcripts by real time PCR analyses at 5 and 10 μM KR12C concentration. ( C ) Western blot analyses of other proteins associated with the signalling cascade (c-MYC, E2F-1, VEGF-A and P53). ( D ) Quatification of protein and mRNA expression level of c-MYC, E2F-1, VEGF-A and P53 by semi-densitometric analyses and real time PCR respectively. Arrow pointing upwards and downwards denote the upregulation of the respective protein and mRNA expression level. Error bars represent mean ± SE ( N = 3). Statistical differences are determined compared to the control by two-tailed Student's t test (* P

    Article Snippet: Santacruz Biotechnology Inc.), anti E2F1, anti VEGF-A, anti-P53, anti-APAF1, anti-Caspase 8, anti-PARP (Abcam), anti-β-actin (Mouse monoclonal, 1:10 000 dil.

    Techniques: Western Blot, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Two Tailed Test

    Schematic representation showing selective quadruplex interaction at c-MYC promoter by KR12C promotes apoptotic signalling in VEGF-A-BCL-2 axis in MCF-7 cells.

    Journal: Nucleic Acids Research

    Article Title: Site-specific amino acid substitution in dodecameric peptides determines the stability and unfolding of c-MYC quadruplex promoting apoptosis in cancer cells

    doi: 10.1093/nar/gky824

    Figure Lengend Snippet: Schematic representation showing selective quadruplex interaction at c-MYC promoter by KR12C promotes apoptotic signalling in VEGF-A-BCL-2 axis in MCF-7 cells.

    Article Snippet: Santacruz Biotechnology Inc.), anti E2F1, anti VEGF-A, anti-P53, anti-APAF1, anti-Caspase 8, anti-PARP (Abcam), anti-β-actin (Mouse monoclonal, 1:10 000 dil.

    Techniques:

    RT-qPCR and Western blot analysis demonstrated that MBNL1-AS1 reduced the expression of KCNMA1 and cGMP-PKG signaling pathway-related genes. a RT-qPCR measured the relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. b Protein bands measured by Western blot analysis. c Western blot analysis measured the relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, p-PKGII phosphorylated-PKGII, VASP vasodilator-stimulated phosphoprotein, p-VASP phosphorylated-VASP, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, si small interfering, GAPDH glyceraldehyde-3-phosphate dehydrogenase, NC negative control; * p

    Journal: Experimental & Molecular Medicine

    Article Title: Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1

    doi: 10.1038/s12276-018-0133-y

    Figure Lengend Snippet: RT-qPCR and Western blot analysis demonstrated that MBNL1-AS1 reduced the expression of KCNMA1 and cGMP-PKG signaling pathway-related genes. a RT-qPCR measured the relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. b Protein bands measured by Western blot analysis. c Western blot analysis measured the relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, p-PKGII phosphorylated-PKGII, VASP vasodilator-stimulated phosphoprotein, p-VASP phosphorylated-VASP, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, si small interfering, GAPDH glyceraldehyde-3-phosphate dehydrogenase, NC negative control; * p

    Article Snippet: The membrane was then blocked in 5% skimmed milk at room temperature for 1 h, washed twice with PBS, and then incubated overnight at 4 °C with diluted rabbit anti-mouse primary antibodies against KCNMA1 (1:1000, MAB8589; AmyJet Scientific Inc., Wuhan, China), PKGII (1:1000, ab145063; Abcam, Inc., Cambridge, MA, USA), VASP (1:1000, ab58555; Abcam, USA), VEGF (1:1000, ab32152; Abcam, USA), Bax (1:1000, ab32503; Abcam, USA), Bcl-2 (1:1000, ab59348; Abcam, USA), Cyclin D1 (1:1000, ab134175, Abcam, USA), Cyclin D3 (1:1000, ab28283, Abcam, USA), Cdc 42 (1:1000, ab187643, Abcam, USA), caspase-3 (1:1000, ab208161, Abcam, USA), and PARP (1:1000, ab32064, Abcam, USA).

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Transfection, Negative Control

    MBNL1-AS1 was highly expressed, but KCNMA1 and cGMP-PKG pathway-related factors were poorly expressed in mice with I/R injury after TKA. a Relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, VASP, VEGF, Bax, and Bcl-2 in the skeletal muscle tissues of mice detected by RT-qPCR. b Protein bands observed after Western blot analysis. c Relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle tissues of mice evaluated by Western blot analysis. I/R ischemia-reperfusion, Sevo sevoflurane, MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, VASP vasodilator-stimulated phosphoprotein, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase; * p

    Journal: Experimental & Molecular Medicine

    Article Title: Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1

    doi: 10.1038/s12276-018-0133-y

    Figure Lengend Snippet: MBNL1-AS1 was highly expressed, but KCNMA1 and cGMP-PKG pathway-related factors were poorly expressed in mice with I/R injury after TKA. a Relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, VASP, VEGF, Bax, and Bcl-2 in the skeletal muscle tissues of mice detected by RT-qPCR. b Protein bands observed after Western blot analysis. c Relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle tissues of mice evaluated by Western blot analysis. I/R ischemia-reperfusion, Sevo sevoflurane, MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, VASP vasodilator-stimulated phosphoprotein, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase; * p

    Article Snippet: The membrane was then blocked in 5% skimmed milk at room temperature for 1 h, washed twice with PBS, and then incubated overnight at 4 °C with diluted rabbit anti-mouse primary antibodies against KCNMA1 (1:1000, MAB8589; AmyJet Scientific Inc., Wuhan, China), PKGII (1:1000, ab145063; Abcam, Inc., Cambridge, MA, USA), VASP (1:1000, ab58555; Abcam, USA), VEGF (1:1000, ab32152; Abcam, USA), Bax (1:1000, ab32503; Abcam, USA), Bcl-2 (1:1000, ab59348; Abcam, USA), Cyclin D1 (1:1000, ab134175, Abcam, USA), Cyclin D3 (1:1000, ab28283, Abcam, USA), Cdc 42 (1:1000, ab187643, Abcam, USA), caspase-3 (1:1000, ab208161, Abcam, USA), and PARP (1:1000, ab32064, Abcam, USA).

    Techniques: Mouse Assay, Expressing, Quantitative RT-PCR, Western Blot

    VEGF-A and VEGFR-1 are important for Snail expression. ( a ) Cells were transfected with indicated vectors. Quantitative RT-PCR (mean±s.d. * P

    Journal: Experimental & Molecular Medicine

    Article Title: Rab25 augments cancer cell invasiveness through a β1 integrin/EGFR/VEGF-A/Snail signaling axis and expression of fascin

    doi: 10.1038/emm.2017.248

    Figure Lengend Snippet: VEGF-A and VEGFR-1 are important for Snail expression. ( a ) Cells were transfected with indicated vectors. Quantitative RT-PCR (mean±s.d. * P

    Article Snippet: Immunohistochemistry was performed with primary Rab25 (ab106175, 1:200, Abcam, Cambridge, MA, USA), VEGFR-1 (ab32152, 1:250, Abcam), Snail (ab180714, 1:100, Abcam) and fascin (ab126772, 1:250, Abcam) antibodies, as described previously.

    Techniques: Expressing, Transfection, Quantitative RT-PCR