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Bender MedSystems vascular endothelial growth factor vegf a
Steel-Dwass test analysis of between-group differences. ( a ) The median serum IP-10 level was significantly higher in the TAFRO-iMCD group than in the other 2 groups. ( b ) The median serum PDGF-AA level was significantly lower in the TAFRO-iMCD group than in controls and tended to be lower in the TAFRO-iMCD groups than in the iMCD-NOS group. The serum IL-10 ( c ), IL-23 ( d ), and <t>VEGF-A</t> ( e ) levels were significantly higher in the TAFRO-iMCD and iMCD-NOS groups than in the control group. Control: Healthy control, iMCD: iMCD-NOS, TAFRO: TAFRO-iMCD, IP-10: chemokine interferon <t>γ-induced</t> protein 10 kDa, PDGF-AA: platelet-derived growth factor -AA, IL: interleukin, VEGF-A; vascular endothelial growth factor-A, N.S: not significant.
Vascular Endothelial Growth Factor Vegf A, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Elevated serum interferon γ-induced protein 10 kDa is associated with TAFRO syndrome"

Article Title: Elevated serum interferon γ-induced protein 10 kDa is associated with TAFRO syndrome

Journal: Scientific Reports

doi: 10.1038/srep42316

Steel-Dwass test analysis of between-group differences. ( a ) The median serum IP-10 level was significantly higher in the TAFRO-iMCD group than in the other 2 groups. ( b ) The median serum PDGF-AA level was significantly lower in the TAFRO-iMCD group than in controls and tended to be lower in the TAFRO-iMCD groups than in the iMCD-NOS group. The serum IL-10 ( c ), IL-23 ( d ), and VEGF-A ( e ) levels were significantly higher in the TAFRO-iMCD and iMCD-NOS groups than in the control group. Control: Healthy control, iMCD: iMCD-NOS, TAFRO: TAFRO-iMCD, IP-10: chemokine interferon γ-induced protein 10 kDa, PDGF-AA: platelet-derived growth factor -AA, IL: interleukin, VEGF-A; vascular endothelial growth factor-A, N.S: not significant.
Figure Legend Snippet: Steel-Dwass test analysis of between-group differences. ( a ) The median serum IP-10 level was significantly higher in the TAFRO-iMCD group than in the other 2 groups. ( b ) The median serum PDGF-AA level was significantly lower in the TAFRO-iMCD group than in controls and tended to be lower in the TAFRO-iMCD groups than in the iMCD-NOS group. The serum IL-10 ( c ), IL-23 ( d ), and VEGF-A ( e ) levels were significantly higher in the TAFRO-iMCD and iMCD-NOS groups than in the control group. Control: Healthy control, iMCD: iMCD-NOS, TAFRO: TAFRO-iMCD, IP-10: chemokine interferon γ-induced protein 10 kDa, PDGF-AA: platelet-derived growth factor -AA, IL: interleukin, VEGF-A; vascular endothelial growth factor-A, N.S: not significant.

Techniques Used: Derivative Assay

Related Articles

Marker:

Article Title: Treatment with the arginase inhibitor Nw-hydroxy-nor-L-arginine restores endothelial function in rat adjuvant-induced arthritis
Article Snippet: .. Plasma IL-6, IL-1β, TNFα and VEGF levels Plasma levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNFα), three peripheral markers of inflammation and plasma concentration of the vascular endothelial growth factor (VEGF), a peripheral marker of endothelial dysfunction in many cardiovascular diseases [ , ], were determined by using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions (Bender MedSystems, Vienna, Austria). .. The limits of detection of ELISA kits provided by the manufacturer for TNF α, IL-6, IL-1β and VEGF were 11.2 pg/mL, 12 pg/mL, 4 pg/mL and 27 pg/mL, respectively.

Concentration Assay:

Article Title: Treatment with the arginase inhibitor Nw-hydroxy-nor-L-arginine restores endothelial function in rat adjuvant-induced arthritis
Article Snippet: .. Plasma IL-6, IL-1β, TNFα and VEGF levels Plasma levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNFα), three peripheral markers of inflammation and plasma concentration of the vascular endothelial growth factor (VEGF), a peripheral marker of endothelial dysfunction in many cardiovascular diseases [ , ], were determined by using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions (Bender MedSystems, Vienna, Austria). .. The limits of detection of ELISA kits provided by the manufacturer for TNF α, IL-6, IL-1β and VEGF were 11.2 pg/mL, 12 pg/mL, 4 pg/mL and 27 pg/mL, respectively.

other:

Article Title: Elevated serum interferon γ-induced protein 10 kDa is associated with TAFRO syndrome
Article Snippet: Measurement of cytokines and chemokines Serum cytokines IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-22, IL-23, IL-27, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, platelet-derived growth factor (PDGF)-AA, vascular endothelial growth factor (VEGF)-A, and chemokine interferon γ-induced protein 10 kDa (IP-10) were analyzed using a FlowCytomix™ kit (Bender MedSystems, Vienna, Austria) and MACSQuant® Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturers’ instructions.

Enzyme-linked Immunosorbent Assay:

Article Title: Treatment with the arginase inhibitor Nw-hydroxy-nor-L-arginine restores endothelial function in rat adjuvant-induced arthritis
Article Snippet: .. Plasma IL-6, IL-1β, TNFα and VEGF levels Plasma levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNFα), three peripheral markers of inflammation and plasma concentration of the vascular endothelial growth factor (VEGF), a peripheral marker of endothelial dysfunction in many cardiovascular diseases [ , ], were determined by using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions (Bender MedSystems, Vienna, Austria). .. The limits of detection of ELISA kits provided by the manufacturer for TNF α, IL-6, IL-1β and VEGF were 11.2 pg/mL, 12 pg/mL, 4 pg/mL and 27 pg/mL, respectively.

Article Title: The effect of Silymarin on VEGF, VEGFR-1 and IL-1? levels in placental cultures of severe preeclamptic women
Article Snippet: .. Samples were then defrosted, and Vascular Endothelial Growth Factor (VEGF), soluble VEGF receptor-1 (sVEGFR-1) and Interleukin (IL)-1α were measured in culture media using an ELISA kit (Bender MedSystems, Austria). .. Statistical analysis of the study data was performed with SPSS 16.0 (SPSS Inc., IL, USA) software.

Article Title: Polyglycolic Acid Fibrous Scaffold Improving Endothelial Cell Coating and Vascularization of Islet
Article Snippet: .. Cytokine detection by enzyme-linked immunosorbant assay Culture supernatants were assayed for vascular endothelial growth factor (VEGF) on days 1, 3, 5, and 7 after EC coating using a commercial ELISA kit (Bender MedSystems, Vienna, Austria). .. Efficiency of endothelial cell-coating of islets An inverted phase contrast microscope was used for real-time monitoring of EC-coating efficiency by comparing the number of ECs on the surface of the islet after staining with dithizone (DTZ; Sigma, St. Louis, USA).

Article Title: Growth Factor Content in Human Sera Affects the Isolation of Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
Article Snippet: .. Vascular endothelial growth factor A (VEGF-A) was quantified by VEGF-A (human) BioLISA Kit Bender MedSystems® (Vienna, Austria) and insulin-like growth factor-1 (IGF-1) by IGF-1 600 ELISA kit from DRG International (Marburg, Germany). ..

Article Title: Cathepsin B inhibition interferes with metastatic potential of human melanoma: an in vitro and in vivo study
Article Snippet: .. Cystatin C and VEGF-A evaluation in the culture medium Evaluation of cystatin C and vascular endothelial growth factor A (VEGF-A) in cell culture medium was performed with specific ELISA kits from Alexis and Bender MedSystem (Lausen, Switzerland), respectively. ..

Cell Culture:

Article Title: Cathepsin B inhibition interferes with metastatic potential of human melanoma: an in vitro and in vivo study
Article Snippet: .. Cystatin C and VEGF-A evaluation in the culture medium Evaluation of cystatin C and vascular endothelial growth factor A (VEGF-A) in cell culture medium was performed with specific ELISA kits from Alexis and Bender MedSystem (Lausen, Switzerland), respectively. ..

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    Bender MedSystems vegf a
    Increased levels of E-selectin detected on the surface of human cerebral microvascular endothelial cells in response to lung tumour CM and secreted-proteins. Confluent hCMEC/D3 cells were stimulated for 4 h with A549 and SK-MES-1 CM ( a ) along with CC ( c ), CL ( d ), IGFBP-7 ( e ), <t>VEGF</t> ( f ) and <t>TNF-α</t> ( g ). E-selectin levels from 3 separate experiments, carried out in quadruplets, were statistically analysed (n ≥ 12, mean ± SD, * p ≤ 0.05 and ** p ≤ 0.01, *** p ≤ 0.005 and **** p ≤ 0.0001). Enhanced E-selectin was also detected within 30 min of CM ( a ) and secreted-proteins ( b ) exposure (* p ≤ 0.05 and ** p ≤ 0.01, n = 6)
    Vegf A, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems vegf c
    <t>VEGF-C</t> and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,
    Vegf C, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems vegf c protein
    Effect of <t>VEGF-C</t> overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p
    Vegf C Protein, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Increased levels of E-selectin detected on the surface of human cerebral microvascular endothelial cells in response to lung tumour CM and secreted-proteins. Confluent hCMEC/D3 cells were stimulated for 4 h with A549 and SK-MES-1 CM ( a ) along with CC ( c ), CL ( d ), IGFBP-7 ( e ), VEGF ( f ) and TNF-α ( g ). E-selectin levels from 3 separate experiments, carried out in quadruplets, were statistically analysed (n ≥ 12, mean ± SD, * p ≤ 0.05 and ** p ≤ 0.01, *** p ≤ 0.005 and **** p ≤ 0.0001). Enhanced E-selectin was also detected within 30 min of CM ( a ) and secreted-proteins ( b ) exposure (* p ≤ 0.05 and ** p ≤ 0.01, n = 6)

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Loss of the endothelial glycocalyx is associated with increased E-selectin mediated adhesion of lung tumour cells to the brain microvascular endothelium

    doi: 10.1186/s13046-015-0223-9

    Figure Lengend Snippet: Increased levels of E-selectin detected on the surface of human cerebral microvascular endothelial cells in response to lung tumour CM and secreted-proteins. Confluent hCMEC/D3 cells were stimulated for 4 h with A549 and SK-MES-1 CM ( a ) along with CC ( c ), CL ( d ), IGFBP-7 ( e ), VEGF ( f ) and TNF-α ( g ). E-selectin levels from 3 separate experiments, carried out in quadruplets, were statistically analysed (n ≥ 12, mean ± SD, * p ≤ 0.05 and ** p ≤ 0.01, *** p ≤ 0.005 and **** p ≤ 0.0001). Enhanced E-selectin was also detected within 30 min of CM ( a ) and secreted-proteins ( b ) exposure (* p ≤ 0.05 and ** p ≤ 0.01, n = 6)

    Article Snippet: Secreted levels of TNF-α, CL, CC, VEGF-A and IGFBP-7 in the A549 and SK-MES-1 CM were measured by ELISA (TNF-α and CC: Bender MedSystems, Vienna, Austria; CL: R & D systems, Abingdon, UK; VEGF-A: PeproTech®, London, UK) and IGFBP-7: in house ELISA previously described in [ ].

    Techniques:

    Lung tumour CM and secreted-proteins enhance adhesion of A549 and SK-MES-1 cells to human brain endothelial monolayers. Confluent hCMEC/D3 cells in μ-slides were exposed to A549 CM or SK-MES-1 CM for 30 min and 24 h prior to perfusion with calcein AM stained A549 ( a ) or SK-MES-1 cells ( c ). A549 and SK-MES-1 adhesion to hCMEC/D3 cells treated with 80 ng/ml CC, 10 ng/ml CL, 200 ng/ml IGFBP-7, 0.2 ng/ml VEGF and 160 pg/ml TNF-α at 4 and 24 h ( b ) and ( d ). Mean values from three separate experiments were statistically analysed. * p ≤ 0.05 versus control levels (100 %). e Representative images of adherent calcein-AM stained A549 [( i ) and ( ii )] and SK-MES-1 [( iii ) and ( iv )] cells to DMEM-BS [( i ) and ( iii )] and CL-treated [( ii ) and ( iv )] brain endothelial monolayers. Scale bar = 75 μm

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Loss of the endothelial glycocalyx is associated with increased E-selectin mediated adhesion of lung tumour cells to the brain microvascular endothelium

    doi: 10.1186/s13046-015-0223-9

    Figure Lengend Snippet: Lung tumour CM and secreted-proteins enhance adhesion of A549 and SK-MES-1 cells to human brain endothelial monolayers. Confluent hCMEC/D3 cells in μ-slides were exposed to A549 CM or SK-MES-1 CM for 30 min and 24 h prior to perfusion with calcein AM stained A549 ( a ) or SK-MES-1 cells ( c ). A549 and SK-MES-1 adhesion to hCMEC/D3 cells treated with 80 ng/ml CC, 10 ng/ml CL, 200 ng/ml IGFBP-7, 0.2 ng/ml VEGF and 160 pg/ml TNF-α at 4 and 24 h ( b ) and ( d ). Mean values from three separate experiments were statistically analysed. * p ≤ 0.05 versus control levels (100 %). e Representative images of adherent calcein-AM stained A549 [( i ) and ( ii )] and SK-MES-1 [( iii ) and ( iv )] cells to DMEM-BS [( i ) and ( iii )] and CL-treated [( ii ) and ( iv )] brain endothelial monolayers. Scale bar = 75 μm

    Article Snippet: Secreted levels of TNF-α, CL, CC, VEGF-A and IGFBP-7 in the A549 and SK-MES-1 CM were measured by ELISA (TNF-α and CC: Bender MedSystems, Vienna, Austria; CL: R & D systems, Abingdon, UK; VEGF-A: PeproTech®, London, UK) and IGFBP-7: in house ELISA previously described in [ ].

    Techniques: Staining

    Exposure of lung tumour CM and secreted-proteins significantly altered the brain endothelial glycocalyx. a and b Integrity of the hCMEC/D3 glycocalyx was assessed by a CBF assay following exposure of ECs for 30 min or 24 h to A549 and SKMES-1 CM ( a ), or 80 ng/ml CC, 10 ng/ml CL, 200 ng/ml IGFBP-7, 0.2 ng/ml VEGF and 160 pg/ml TNF-α ( b ). Data from 6 independent experiments, carried out in sextuplicate, is presented as WGA-FITC fluorescence/μg protein and these values were calculated as a percentage of control. * p ≤ 0.05, ** p ≤ 0.01 vs control level. c Hyaluronan (HA) and syndecan-1 levels were measured by ELISA in hCMEC/D3 growth medium following 30 min treatment with fresh DMEM-BS (control), A549 or SK-MES-1 CM. SK-MES-1 CM vs control = p = 0.007, n = 6. nd = not detected. d and c Representative confocal images of FITC-WGA stained hCMEC/D3 cells before ( d ) and ( e ) after 30 min exposure to SKMES-1 CM. Scale bar = 40 μM

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Loss of the endothelial glycocalyx is associated with increased E-selectin mediated adhesion of lung tumour cells to the brain microvascular endothelium

    doi: 10.1186/s13046-015-0223-9

    Figure Lengend Snippet: Exposure of lung tumour CM and secreted-proteins significantly altered the brain endothelial glycocalyx. a and b Integrity of the hCMEC/D3 glycocalyx was assessed by a CBF assay following exposure of ECs for 30 min or 24 h to A549 and SKMES-1 CM ( a ), or 80 ng/ml CC, 10 ng/ml CL, 200 ng/ml IGFBP-7, 0.2 ng/ml VEGF and 160 pg/ml TNF-α ( b ). Data from 6 independent experiments, carried out in sextuplicate, is presented as WGA-FITC fluorescence/μg protein and these values were calculated as a percentage of control. * p ≤ 0.05, ** p ≤ 0.01 vs control level. c Hyaluronan (HA) and syndecan-1 levels were measured by ELISA in hCMEC/D3 growth medium following 30 min treatment with fresh DMEM-BS (control), A549 or SK-MES-1 CM. SK-MES-1 CM vs control = p = 0.007, n = 6. nd = not detected. d and c Representative confocal images of FITC-WGA stained hCMEC/D3 cells before ( d ) and ( e ) after 30 min exposure to SKMES-1 CM. Scale bar = 40 μM

    Article Snippet: Secreted levels of TNF-α, CL, CC, VEGF-A and IGFBP-7 in the A549 and SK-MES-1 CM were measured by ELISA (TNF-α and CC: Bender MedSystems, Vienna, Austria; CL: R & D systems, Abingdon, UK; VEGF-A: PeproTech®, London, UK) and IGFBP-7: in house ELISA previously described in [ ].

    Techniques: Whole Genome Amplification, Fluorescence, Enzyme-linked Immunosorbent Assay, Staining

    Reverse transcription–polymerase chain reaction (RT–PCR) analysis of VEGF-A and VEGF-C. RT–PCR analysis of VEGF-A and VEGF-C mRNA expression, respectively, in ARPE-19 A and HECV B cells cultured 24 h in standard conditions (CTR) or in the presence of AGEs (GS). GAPDH was used as the internal control. VEGF-A and VEGF-C secretion in ARPE-19 C , E: and HECV D , F: cells, respectively, after 24 h culture in CTR or in presence of AGEs (GS). The conditioned media were collected, and ELISA was performed. Concentrations of VEGFs were calculated from standards curves and normalized to total protein. The results are representative of at least three experiments (mean±SEM). *p

    Journal: Molecular Vision

    Article Title: Vascular endothelial growth factor-C secretion is increased by advanced glycation end-products: possible implication in ocular neovascularization

    doi:

    Figure Lengend Snippet: Reverse transcription–polymerase chain reaction (RT–PCR) analysis of VEGF-A and VEGF-C. RT–PCR analysis of VEGF-A and VEGF-C mRNA expression, respectively, in ARPE-19 A and HECV B cells cultured 24 h in standard conditions (CTR) or in the presence of AGEs (GS). GAPDH was used as the internal control. VEGF-A and VEGF-C secretion in ARPE-19 C , E: and HECV D , F: cells, respectively, after 24 h culture in CTR or in presence of AGEs (GS). The conditioned media were collected, and ELISA was performed. Concentrations of VEGFs were calculated from standards curves and normalized to total protein. The results are representative of at least three experiments (mean±SEM). *p

    Article Snippet: VEGF-A and VEGF-C secretions were assessed with enzyme-linked immunosorbent assay (ELISA; Bender MedSystem, Vienna, Austria).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

    Secretion of vascular endothelial growth factor (VEGF)-A and –C. VEGF-A and VEGF-C secretion in ARPE-19 ( A , C and HECV B , D cells, respectively, transfected with a scrambled siRNA sequence (siControl) or siRNA of RAGE (siRAGE) following 24 h of culture in standard conditions (CTR) or in the presence of AGEs (GS).The conditioned media were collected, and ELISA was performed. Concentrations of VEGFs were calculated from standards curves and normalized to total protein. The results are representative of at least three experiments (mean±SEM). **p

    Journal: Molecular Vision

    Article Title: Vascular endothelial growth factor-C secretion is increased by advanced glycation end-products: possible implication in ocular neovascularization

    doi:

    Figure Lengend Snippet: Secretion of vascular endothelial growth factor (VEGF)-A and –C. VEGF-A and VEGF-C secretion in ARPE-19 ( A , C and HECV B , D cells, respectively, transfected with a scrambled siRNA sequence (siControl) or siRNA of RAGE (siRAGE) following 24 h of culture in standard conditions (CTR) or in the presence of AGEs (GS).The conditioned media were collected, and ELISA was performed. Concentrations of VEGFs were calculated from standards curves and normalized to total protein. The results are representative of at least three experiments (mean±SEM). **p

    Article Snippet: VEGF-A and VEGF-C secretions were assessed with enzyme-linked immunosorbent assay (ELISA; Bender MedSystem, Vienna, Austria).

    Techniques: Transfection, Sequencing, Enzyme-linked Immunosorbent Assay

    VEGF-C and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,

    Journal: Journal of Clinical Investigation

    Article Title: Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation

    doi: 10.1172/JCI200522037

    Figure Lengend Snippet: VEGF-C and VEGF-D in M. pulmonis –infected airways. Immunohistochemical staining of VEGF-C ( A – C ) and VEGF-D ( D – F ) in mouse airways and lung 14 days after infection. ( A ) VEGF-C (green) in epithelium, peribronchial inflammatory cells,

    Article Snippet: VEGF-C and VEGF-D in the lungs of nude mice treated intranasally with adenoviruses encoding LacZ, VEGF165, VEGF-C, or VEGF-D ΔNΔC 10 days previously were measured by ELISA (human VEGF-C ELISA [no. BMS297; Bender MedSystems], human VEGF-D ELISA [no. DVED00; R & D Systems]).

    Techniques: Infection, Immunohistochemistry, Staining

    Effect of VEGF-C overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of VEGF-C overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Over Expression, Staining

    Effect of VEGFR3-Ig treatment on orthotopic PC-3/VEGF-C tumors . VEGFR3-Ig fusion protein-producing construct or lacZ-Ig controls were injected intravenously into mice five minutes before orthotopic inoculation of PC-3/VEGF-C cells. The density of m-LYVE-1 positive lymphatic capillaries was decreased in VEGFR3-Ig-treated tumors (23 ± 4 μm/mm 2 , n = 8 vs . 123 ± 7 μm/mm 2 , n = 6, p

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of VEGFR3-Ig treatment on orthotopic PC-3/VEGF-C tumors . VEGFR3-Ig fusion protein-producing construct or lacZ-Ig controls were injected intravenously into mice five minutes before orthotopic inoculation of PC-3/VEGF-C cells. The density of m-LYVE-1 positive lymphatic capillaries was decreased in VEGFR3-Ig-treated tumors (23 ± 4 μm/mm 2 , n = 8 vs . 123 ± 7 μm/mm 2 , n = 6, p

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Construct, Injection, Mouse Assay

    Ectopic expression of VEGF-C in PC-3 cells and orthotopic tumors . PC-3 cells were stably transfected with a vector containing human VEGF-C (PC-3/VEGF-C) or empty vector (mock). One cell clone expressing VEGF-C at a high level, and one control clone (PC-3/mock) were selected for further studies. ELISA analysis of VEGF-C protein concentration in cell lysates (A) and in conditioned media (B) revealed increased expression in PC-3/VEGF-C cells compared with PC-3/mock cells. Data are expressed as means ± SD ng/mL, corresponding 10 6 cells. (C) Western blot analysis revealed that PC-3 cell clones expressed mainly 29/31 kD form of VEGF-C. Detectable levels of 21 kD form were not seen. β-actin control was from cell lysates corresponding to the collected conditioned medium.

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Ectopic expression of VEGF-C in PC-3 cells and orthotopic tumors . PC-3 cells were stably transfected with a vector containing human VEGF-C (PC-3/VEGF-C) or empty vector (mock). One cell clone expressing VEGF-C at a high level, and one control clone (PC-3/mock) were selected for further studies. ELISA analysis of VEGF-C protein concentration in cell lysates (A) and in conditioned media (B) revealed increased expression in PC-3/VEGF-C cells compared with PC-3/mock cells. Data are expressed as means ± SD ng/mL, corresponding 10 6 cells. (C) Western blot analysis revealed that PC-3 cell clones expressed mainly 29/31 kD form of VEGF-C. Detectable levels of 21 kD form were not seen. β-actin control was from cell lysates corresponding to the collected conditioned medium.

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Protein Concentration, Western Blot, Clone Assay

    Effect of VEGF-C overexpression on PC-3 prostate tumor growth . The occurrence of subcutaneous tumors of both PC-3/VEGF-C ( n = 8) and PC-3/mock ( n = 12) was 100%. (A) Subcutaneous PC-3/VEGF-C tumors grew larger (1132 ± 34 mm 3 , gray bars) than PC-3/mock tumors (47 ± 7 mm 3 , black bars) at 4 weeks and the differences in tumor volumes between the groups were significant at all time points between 2 and 4 weeks ( p

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of VEGF-C overexpression on PC-3 prostate tumor growth . The occurrence of subcutaneous tumors of both PC-3/VEGF-C ( n = 8) and PC-3/mock ( n = 12) was 100%. (A) Subcutaneous PC-3/VEGF-C tumors grew larger (1132 ± 34 mm 3 , gray bars) than PC-3/mock tumors (47 ± 7 mm 3 , black bars) at 4 weeks and the differences in tumor volumes between the groups were significant at all time points between 2 and 4 weeks ( p

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Over Expression

    Effect of ectopic VEGF-C on lymphatic vessel density of orthotopic PC-3 tumors . M-LYVE-1-positive staining was observed primarily in the tumor periphery and in the peritumoral area (A-B). No differences were detected in the density of lymphatic vessels Bar 500 μm.

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of ectopic VEGF-C on lymphatic vessel density of orthotopic PC-3 tumors . M-LYVE-1-positive staining was observed primarily in the tumor periphery and in the peritumoral area (A-B). No differences were detected in the density of lymphatic vessels Bar 500 μm.

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Staining

    Metastasis of PC-3/VEGF-C tumors to prostate-draining lymph nodes and lungs . The prostate draining lymph nodes and the lungs were cut through to detect metastasis. Altogether 8-10 lymph nodes and six lung sections/mouse were studied and number of metastases was counted. The relative metastatic area of lymph nodes was determined by histomorphometry but there were no statistically significant differences (data not shown). The occurrence of metastasis in lymph nodes was 21% ( n = 6 mice out of 29 tumor-bearing mice) in the PC-3/VEGF-C group and 58% ( n = 14 mice out of 24 tumor-bearing mice) in the PC-3/mock group and in lungs was 48% ( n = 14 out of 29 tumor bearing mice) in the PC-3/VEGF-C group and 8% ( n = 2 out of 24 tumor-bearing mice) in the PC-3/mock group.

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Metastasis of PC-3/VEGF-C tumors to prostate-draining lymph nodes and lungs . The prostate draining lymph nodes and the lungs were cut through to detect metastasis. Altogether 8-10 lymph nodes and six lung sections/mouse were studied and number of metastases was counted. The relative metastatic area of lymph nodes was determined by histomorphometry but there were no statistically significant differences (data not shown). The occurrence of metastasis in lymph nodes was 21% ( n = 6 mice out of 29 tumor-bearing mice) in the PC-3/VEGF-C group and 58% ( n = 14 mice out of 24 tumor-bearing mice) in the PC-3/mock group and in lungs was 48% ( n = 14 out of 29 tumor bearing mice) in the PC-3/VEGF-C group and 8% ( n = 2 out of 24 tumor-bearing mice) in the PC-3/mock group.

    Article Snippet: VEGF-C protein concentrations from cell lysates and 24 h conditioned media of cell clones were further quantified by ELISA (Bender MedSystems, Vienna, Austria) according to the manufacturer's instructions.

    Techniques: Mouse Assay