vana vancomycin resistant e faecium  (ATCC)


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    Name:
    Enterococcus faecium
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    Catalog Number:
    baa-2317
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    Structured Review

    ATCC vana vancomycin resistant e faecium
    Reverse-phase HPLC analysis of peptidoglycan precursor UDPMurNAc-pp ( 19 ) in <t>VanA</t> VRE ( E. <t>faecium</t> ATCC BAA-2317).

    https://www.bioz.com/result/vana vancomycin resistant e faecium/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vana vancomycin resistant e faecium - by Bioz Stars, 2020-03
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    bacterial cell membrane depolarization

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    1) Product Images from "Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics"

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1704125114

    Reverse-phase HPLC analysis of peptidoglycan precursor UDPMurNAc-pp ( 19 ) in VanA VRE ( E. faecium ATCC BAA-2317).
    Figure Legend Snippet: Reverse-phase HPLC analysis of peptidoglycan precursor UDPMurNAc-pp ( 19 ) in VanA VRE ( E. faecium ATCC BAA-2317).

    Techniques Used: High Performance Liquid Chromatography

    Examination of cell wall permeability induced by compounds 9 − 13 (10 μM added at 5 min) in VanA VRE ( E. faecium ATCC BAA-2317).
    Figure Legend Snippet: Examination of cell wall permeability induced by compounds 9 − 13 (10 μM added at 5 min) in VanA VRE ( E. faecium ATCC BAA-2317).

    Techniques Used: Permeability

    Examination of cell wall permeability induced by compounds 14 − 18 (10 μM added at 5 min) in VanA VRE ( E. faecium ATCC BAA-2317).
    Figure Legend Snippet: Examination of cell wall permeability induced by compounds 14 − 18 (10 μM added at 5 min) in VanA VRE ( E. faecium ATCC BAA-2317).

    Techniques Used: Permeability

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: The entire supernatant was analyzed by semipreparative reverse-phase HPLC with a single injection, and the amount of 19 was quantitated with use of the calibration curve. .. The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays.

    Centrifugation:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: The test compounds were incubated with tetracycline-pretreated VanA VRE for 30 min (37 °C) before the cultured bacteria were collected by centrifugation, washed, and resuspended in pH 7.2 buffer (5 mM Hepes:5 mM glucose; 1:1). .. The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays.

    Permeability:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays. .. The effect of the compounds on cell membrane permeability was examined by measuring cytoplasmic membrane uptake of the fluorescent probe propidium iodide in the same VanA vancomycin-resistant E. faecalis (BM 4166) ( ) and E. faecium (ATCC BAA-2317) strains.

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: .. The effect of the compounds on cell membrane permeability was examined by measuring cytoplasmic membrane uptake of the fluorescent probe propidium iodide in the same VanA vancomycin-resistant E. faecalis (BM 4166) ( ) and E. faecium (ATCC BAA-2317) strains. .. This fluorescent probe only enters cells with permeabilized cell membranes and is detected by the emission of fluorescence on intracellular nucleic acid binding ( ).

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ). .. Because the C1 introduction is such a small and seemingly benign structural modification and because it induces membrane permeability without membrane depolarization or cell wall lysis, it suggests a more specific mechanism of action.

    Fluorescence:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays. .. This fluorescent probe only enters cells with permeabilized cell membranes and is detected by the emission of fluorescence on intracellular nucleic acid binding ( ).

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: .. Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ). .. Because the C1 introduction is such a small and seemingly benign structural modification and because it induces membrane permeability without membrane depolarization or cell wall lysis, it suggests a more specific mechanism of action.

    Isolation:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: A larger-scale isolation and full characterization of 19 ( ) provided the material needed to establish a linear calibration curve ( ). .. The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays.

    Cell Culture:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: The test compounds were incubated with tetracycline-pretreated VanA VRE for 30 min (37 °C) before the cultured bacteria were collected by centrifugation, washed, and resuspended in pH 7.2 buffer (5 mM Hepes:5 mM glucose; 1:1). .. The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays.

    Modification:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: This procedure was reduced in scale and modified to enlist reverse-phase HPLC separation, UV detection, and calibration curve determination of the amount of isolated 19 , permitting use with limited sample sizes ( ). .. The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays.

    Article Title: Total Syntheses and Initial Evaluation of [Ψ[C(=S)NH]Tpg4]vancomycin, [Ψ[C(=NH)NH]Tpg4]vancomycin, [Ψ[CH2NH]Tpg4]vancomycin and their (4-Chlorobiphenyl)methyl Derivatives: Synergistic Binding Pocket and Peripheral Modifications for the Glycopeptide Antibiotics
    Article Snippet: The pocket modified vancomycin analogues that contain the C-terminus hydroxymethyl group ( 23 – 25 ), their chlorobiphenyl derivatives ( 26 – 28 ), as well as the fully functionalized vancomycin analogues [Ψ[C(=S)NH]Tpg4 ]vancomycin, [Ψ[C(=NH)NH]Tpg4 ]vancomycin, and [Ψ[CH2 NH]Tpg4 ]vancomycin ( 33 – 35 ) and their (4-chlorobiphenyl)methyl derivatives ( 37 – 39 ) were examined alongside the corresponding vancomycin (residue 4 amide) derivatives. .. Notably, one VanA VRE ( E. faecium , ATCC BAA-2317) tested represents an emerging challenging multidrug resistant VanA VRE that is not only resistant to vancomycin and teicoplanin, but also ampicillin, benzylpenicillin, ciprofloxacin, erythromycin, levofloxacin, nitrofurantoin, tetracycline.

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ). .. Because the C1 introduction is such a small and seemingly benign structural modification and because it induces membrane permeability without membrane depolarization or cell wall lysis, it suggests a more specific mechanism of action.

    Incubation:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: The test compounds were incubated with tetracycline-pretreated VanA VRE for 30 min (37 °C) before the cultured bacteria were collected by centrifugation, washed, and resuspended in pH 7.2 buffer (5 mM Hepes:5 mM glucose; 1:1). .. The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays.

    Inhibition:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: .. Full details of the bacterial cell growth inhibition assays with vancomycin-resistant E. faecalis (VanA VRE; BM4166), E. faecium (VanA VRE; ATCC BAA-2317), and vancomycin-resistant E. faecalis (VanB VRE; strain ATCC 51299) are provided in . ..

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: No growth inhibition (cytotoxic activity) was observed up to 100 μM, the highest dose tested. .. Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ).

    Activity Assay:

    Article Title: Total Syntheses and Initial Evaluation of [Ψ[C(=S)NH]Tpg4]vancomycin, [Ψ[C(=NH)NH]Tpg4]vancomycin, [Ψ[CH2NH]Tpg4]vancomycin and their (4-Chlorobiphenyl)methyl Derivatives: Synergistic Binding Pocket and Peripheral Modifications for the Glycopeptide Antibiotics
    Article Snippet: Paragraph title: Antimicrobial activity ... Notably, one VanA VRE ( E. faecium , ATCC BAA-2317) tested represents an emerging challenging multidrug resistant VanA VRE that is not only resistant to vancomycin and teicoplanin, but also ampicillin, benzylpenicillin, ciprofloxacin, erythromycin, levofloxacin, nitrofurantoin, tetracycline.

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: Finally, no correlation in activity with cLogP was found for the series of compounds studied, and none of the compounds ( 5 , 12 , 13 , 15 , 17 , and 18 ) exhibited aggregation, higher-order complex formation, or micelle formation at concentrations up to 100 μM in PBS buffer (25 °C), indicating that such effects are not playing a role in the expression of the activity of the compounds. .. Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ).

    Expressing:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: Finally, no correlation in activity with cLogP was found for the series of compounds studied, and none of the compounds ( 5 , 12 , 13 , 15 , 17 , and 18 ) exhibited aggregation, higher-order complex formation, or micelle formation at concentrations up to 100 μM in PBS buffer (25 °C), indicating that such effects are not playing a role in the expression of the activity of the compounds. .. Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ).

    Sequencing:

    Article Title: Total Syntheses and Initial Evaluation of [Ψ[C(=S)NH]Tpg4]vancomycin, [Ψ[C(=NH)NH]Tpg4]vancomycin, [Ψ[CH2NH]Tpg4]vancomycin and their (4-Chlorobiphenyl)methyl Derivatives: Synergistic Binding Pocket and Peripheral Modifications for the Glycopeptide Antibiotics
    Article Snippet: The sequence was conducted only twice on a preparative scale and consequently is not yet optimized. .. VanA E. faecium (VanA VRE, ATCC BAA-2317): resistant to ampicillin, benzylpenicillin, ciprofloxacin, erythromycin, levofloxacin, nitrofurantoin, and tetracycline as well as vancomycin and teicoplanin, insensitive to linezolid; sensitive to tigecycline and dalfopristine.

    Lysis:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ). .. Because the C1 introduction is such a small and seemingly benign structural modification and because it induces membrane permeability without membrane depolarization or cell wall lysis, it suggests a more specific mechanism of action.

    Injection:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: The entire supernatant was analyzed by semipreparative reverse-phase HPLC with a single injection, and the amount of 19 was quantitated with use of the calibration curve. .. The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays.

    Binding Assay:

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics
    Article Snippet: The results of the evaluation of 5 – 18 in VanA vancomycin-resistant Enterococcus faecalis (BM 4166) and E. faecium (ATCC BAA-2317) are presented in and represent the same strains used in the antimicrobial assays. .. This fluorescent probe only enters cells with permeabilized cell membranes and is detected by the emission of fluorescence on intracellular nucleic acid binding ( ).

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  • 99
    ATCC vana vancomycin resistant e faecium
    Reverse-phase HPLC analysis of peptidoglycan precursor UDPMurNAc-pp ( 19 ) in <t>VanA</t> VRE ( E. <t>faecium</t> ATCC BAA-2317).
    Vana Vancomycin Resistant E Faecium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vana vancomycin resistant e faecium/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vana vancomycin resistant e faecium - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    ATCC e faecium
    Reverse-phase HPLC analysis of peptidoglycan precursor UDPMurNAc-pp ( 19 ) in <t>VanA</t> VRE ( E. <t>faecium</t> ATCC BAA-2317).
    E Faecium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e faecium/product/ATCC
    Average 99 stars, based on 7 article reviews
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    e faecium - by Bioz Stars, 2020-03
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    77
    ATCC vancomycin resistant e faecium strains
    Reverse-phase HPLC analysis of peptidoglycan precursor UDPMurNAc-pp ( 19 ) in <t>VanA</t> VRE ( E. <t>faecium</t> ATCC BAA-2317).
    Vancomycin Resistant E Faecium Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vancomycin resistant e faecium strains/product/ATCC
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vancomycin resistant e faecium strains - by Bioz Stars, 2020-03
    77/100 stars
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    Reverse-phase HPLC analysis of peptidoglycan precursor UDPMurNAc-pp ( 19 ) in VanA VRE ( E. faecium ATCC BAA-2317).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics

    doi: 10.1073/pnas.1704125114

    Figure Lengend Snippet: Reverse-phase HPLC analysis of peptidoglycan precursor UDPMurNAc-pp ( 19 ) in VanA VRE ( E. faecium ATCC BAA-2317).

    Article Snippet: Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ).

    Techniques: High Performance Liquid Chromatography

    Examination of cell wall permeability induced by compounds 9 − 13 (10 μM added at 5 min) in VanA VRE ( E. faecium ATCC BAA-2317).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics

    doi: 10.1073/pnas.1704125114

    Figure Lengend Snippet: Examination of cell wall permeability induced by compounds 9 − 13 (10 μM added at 5 min) in VanA VRE ( E. faecium ATCC BAA-2317).

    Article Snippet: Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ).

    Techniques: Permeability

    Examination of cell wall permeability induced by compounds 14 − 18 (10 μM added at 5 min) in VanA VRE ( E. faecium ATCC BAA-2317).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Peripheral modifications of [Ψ[CH2NH]Tpg4]vancomycin with added synergistic mechanisms of action provide durable and potent antibiotics

    doi: 10.1073/pnas.1704125114

    Figure Lengend Snippet: Examination of cell wall permeability induced by compounds 14 − 18 (10 μM added at 5 min) in VanA VRE ( E. faecium ATCC BAA-2317).

    Article Snippet: Compound 18 also failed to produce bacterial cell membrane depolarization in the same VanA vancomycin-resistant E. faecium (ATCC BAA-2317) as measured by fluorescence of a released membrane imbedded dye (DiSC3 5,3,3′-dipropylthiadicarbocyanine iodide) ( ) ( ).

    Techniques: Permeability