Structured Review

Thermo Fisher v1
V1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/v1/product/Thermo Fisher
Average 91 stars, based on 4 article reviews
Price from $9.99 to $1999.99
v1 - by Bioz Stars, 2020-07
91/100 stars

Images

Related Articles

Nucleic Acid Electrophoresis:

Article Title: Protein-Primed and De Novo Initiation of RNA Synthesis by Norovirus 3Dpol
Article Snippet: .. To further assess the specificity of the elongation reaction, the elongated VPg-poly(U) was submitted to digestion with RNases A and V1 (Ambion) at final concentrations of 1 μg/μl and 10 U/μl, respectively, for 2 h. The digested VPg-poly(U) was then analyzed by SDS-polyacrylamide gel electrophoresis (PAGE). .. The 5′ end of the 3Dpol replication product was mapped using an RNA ligase-mediated rapid amplification of cDNA ends approach.

Binding Assay:

Article Title: Hfq restructures RNA-IN and RNA-OUT and facilitates antisense pairing in the Tn10/IS10 system
Article Snippet: .. Binding reactions took place at 37°C for 15 min. For RNase footprinting, 1 μL of dilute RNAse A, T1, or V1 (Ambion) was added to each binding reaction. ..

Polyacrylamide Gel Electrophoresis:

Article Title: Protein-Primed and De Novo Initiation of RNA Synthesis by Norovirus 3Dpol
Article Snippet: .. To further assess the specificity of the elongation reaction, the elongated VPg-poly(U) was submitted to digestion with RNases A and V1 (Ambion) at final concentrations of 1 μg/μl and 10 U/μl, respectively, for 2 h. The digested VPg-poly(U) was then analyzed by SDS-polyacrylamide gel electrophoresis (PAGE). .. The 5′ end of the 3Dpol replication product was mapped using an RNA ligase-mediated rapid amplification of cDNA ends approach.

Footprinting:

Article Title: Hfq restructures RNA-IN and RNA-OUT and facilitates antisense pairing in the Tn10/IS10 system
Article Snippet: .. Binding reactions took place at 37°C for 15 min. For RNase footprinting, 1 μL of dilute RNAse A, T1, or V1 (Ambion) was added to each binding reaction. ..

Purification:

Article Title: IL-17 receptor-associated adaptor Act1 directly stabilizes mRNAs to mediate IL-17 inflammatory signaling
Article Snippet: .. The indicated amounts of RNase T1, A, or V1 (Ambion) were added to the appropriate samples and incubated at 22°C for 5 min. Enzymatic reactions were quenched with 30 μL Inactivation/Precipitation buffer (Ambion) and purified according to manufacturer’s directions. .. Samples were resuspended in 10 μL of loading buffer (Ambion), heat-denatured at 95°C for 5 min, and separated in a denaturing 8% (19:1) polyacrylamide/7 M urea gel.

Incubation:

Article Title: An AU-rich stem-loop structure is a critical feature of the perinuclear localization signal of c-myc mRNA
Article Snippet: .. 105 c.p.m.) was supplemented with 2–5 μg of yeast tRNA and incubated separately with RNase T1 (1 unit/μl, Ambion), V1 (0.1 unit/μl, Ambion), T2 (30 units/μl, Sigma) or A (1 μg/μl, Ambion) at room temperature in 20 mM Tris/HCl (pH 7.5), 10 mM MgCl2 , 100 mM KCl according to the conditions indicated in the legend of the Figures. .. For lead-mediated cleavage, RNA was supplemented with 4 μg of yeast tRNA and incubated for 5 min at room temperature with 2 or 5 mM Pb2+ in 20 mM Hepes/NaOH (pH 7.5), 7 mM magnesium acetate, 50 mM potassium acetate.

Article Title: IL-17 receptor-associated adaptor Act1 directly stabilizes mRNAs to mediate IL-17 inflammatory signaling
Article Snippet: .. The indicated amounts of RNase T1, A, or V1 (Ambion) were added to the appropriate samples and incubated at 22°C for 5 min. Enzymatic reactions were quenched with 30 μL Inactivation/Precipitation buffer (Ambion) and purified according to manufacturer’s directions. .. Samples were resuspended in 10 μL of loading buffer (Ambion), heat-denatured at 95°C for 5 min, and separated in a denaturing 8% (19:1) polyacrylamide/7 M urea gel.

Article Title: Analysis of hepatitis C virus RNA dimerization and core-RNA interactions
Article Snippet: .. RNA was incubated with RNases T1 (Life Technologies), T2 (Life Technologies) or V1 (Ambion) for 7 min at 37°C. ..

Expressing:

Article Title: New miRNA labeling method for bead-based quantification
Article Snippet: .. To better understand the global function of miRNA in RMS, we analyzed the expression profile of 7 different RMS cell lines (3 ARMS and 4 ERMS) using the mirVana miRNA Probe Set V1 (Ambion) that is a collection of about 400 amino-modified DNA oligonucleotides [ ]. ..

Western Blot:

Article Title: Antisense RNA Controls LRP1 Sense Transcript Expression Through Interaction With a Chromatin-Associated Protein, HMGB2
Article Snippet: .. After five washes, bound proteins were eluted with RNase A/T1 and V1 (Ambion), and visualized by Silver Staining Kit (GE Healthcare) or detected by Western blotting. ..

Silver Staining:

Article Title: Antisense RNA Controls LRP1 Sense Transcript Expression Through Interaction With a Chromatin-Associated Protein, HMGB2
Article Snippet: .. After five washes, bound proteins were eluted with RNase A/T1 and V1 (Ambion), and visualized by Silver Staining Kit (GE Healthcare) or detected by Western blotting. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Thermo Fisher protein thermal shift software v1 0
    Protein thermal shift assay (PTSA) and mutagenesis to determine essential residues of NS3 for binding of EB ( A ) PTSA. Experimental details were described in the Method section. Experimental data were fitted and analyzed by using the Protein Thermal Shift ™ Software <t>v1.0</t> (ThermoFisher Scientific). ΔTm = T m-EB – T m-DMSO . N = 3. ( B ) Protease activity of each mutant NS3 protein relative to that of WT. All proteins were at 150 nM. N = 3. ***, p
    Protein Thermal Shift Software V1 0, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein thermal shift software v1 0/product/Thermo Fisher
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    protein thermal shift software v1 0 - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    92
    Thermo Fisher rnase v1
    Addition of CA-repeats does not change the secondary structure of the coat RBS stem–loop. ( A ) Enzymatic and chemical structure probing was conducted on control mRNA (00) or mRNAs with tails of 4, 6 or 8 (CA)-repeats, (see Materials and Methods), as indicated. The mRNAs were mock-treated (lanes ‘–’), partially digested with double-strand-specific <t>RNase</t> V1 (V1), or treated with lead(II) acetate (Pb 2+ ). UCGA: sequencing reactions on (CA)8 mRNA. The position of the SD and AUG start codon are indicated by red boxes. Regions of reactivity toward RNase V1 (red solid line) and lead(II) acetate (red dashed line) are indicated on the autoradiogram. ( B ) The localization of RNase V1 (filled triangles) and lead(II) acetate (black dots) cuts are shown on the secondary structure of the 5′-segment of (CA)8 mRNA. Black boxes: SD and AUG.
    Rnase V1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase v1/product/Thermo Fisher
    Average 92 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    rnase v1 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Thermo Fisher proteome discoverer v1 4
    Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive Orbitrap mass spectrometer. Raw data generated from the mass spectrometer were processed using <t>Proteome</t> Discover <t>v1.4</t> and Mascot. The search results were further analysed by the TMTPrePro software package.
    Proteome Discoverer V1 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteome discoverer v1 4/product/Thermo Fisher
    Average 91 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    proteome discoverer v1 4 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    Protein thermal shift assay (PTSA) and mutagenesis to determine essential residues of NS3 for binding of EB ( A ) PTSA. Experimental details were described in the Method section. Experimental data were fitted and analyzed by using the Protein Thermal Shift ™ Software v1.0 (ThermoFisher Scientific). ΔTm = T m-EB – T m-DMSO . N = 3. ( B ) Protease activity of each mutant NS3 protein relative to that of WT. All proteins were at 150 nM. N = 3. ***, p

    Journal: Antiviral research

    Article Title: Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease

    doi: 10.1016/j.antiviral.2017.12.018

    Figure Lengend Snippet: Protein thermal shift assay (PTSA) and mutagenesis to determine essential residues of NS3 for binding of EB ( A ) PTSA. Experimental details were described in the Method section. Experimental data were fitted and analyzed by using the Protein Thermal Shift ™ Software v1.0 (ThermoFisher Scientific). ΔTm = T m-EB – T m-DMSO . N = 3. ( B ) Protease activity of each mutant NS3 protein relative to that of WT. All proteins were at 150 nM. N = 3. ***, p

    Article Snippet: The melting temperature (Tm ) was calculated as the mid-log of the transition phase from the native to the denatured protein, using a derivative model in the Protein Thermal Shift™ Software v1.0 (ThermoFisher Scientific).

    Techniques: Thermal Shift Assay, Mutagenesis, Binding Assay, Software, Activity Assay

    Addition of CA-repeats does not change the secondary structure of the coat RBS stem–loop. ( A ) Enzymatic and chemical structure probing was conducted on control mRNA (00) or mRNAs with tails of 4, 6 or 8 (CA)-repeats, (see Materials and Methods), as indicated. The mRNAs were mock-treated (lanes ‘–’), partially digested with double-strand-specific RNase V1 (V1), or treated with lead(II) acetate (Pb 2+ ). UCGA: sequencing reactions on (CA)8 mRNA. The position of the SD and AUG start codon are indicated by red boxes. Regions of reactivity toward RNase V1 (red solid line) and lead(II) acetate (red dashed line) are indicated on the autoradiogram. ( B ) The localization of RNase V1 (filled triangles) and lead(II) acetate (black dots) cuts are shown on the secondary structure of the 5′-segment of (CA)8 mRNA. Black boxes: SD and AUG.

    Journal: Nucleic Acids Research

    Article Title: Unstructured 5′-tails act through ribosome standby to override inhibitory structure at ribosome binding sites

    doi: 10.1093/nar/gky073

    Figure Lengend Snippet: Addition of CA-repeats does not change the secondary structure of the coat RBS stem–loop. ( A ) Enzymatic and chemical structure probing was conducted on control mRNA (00) or mRNAs with tails of 4, 6 or 8 (CA)-repeats, (see Materials and Methods), as indicated. The mRNAs were mock-treated (lanes ‘–’), partially digested with double-strand-specific RNase V1 (V1), or treated with lead(II) acetate (Pb 2+ ). UCGA: sequencing reactions on (CA)8 mRNA. The position of the SD and AUG start codon are indicated by red boxes. Regions of reactivity toward RNase V1 (red solid line) and lead(II) acetate (red dashed line) are indicated on the autoradiogram. ( B ) The localization of RNase V1 (filled triangles) and lead(II) acetate (black dots) cuts are shown on the secondary structure of the 5′-segment of (CA)8 mRNA. Black boxes: SD and AUG.

    Article Snippet: RNase V1 probing used a final concentration of 0.01 U/μl (ThermoFisher Scientific, #AM2275) for 5 min at 37°C.

    Techniques: Sequencing

    Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive Orbitrap mass spectrometer. Raw data generated from the mass spectrometer were processed using Proteome Discover v1.4 and Mascot. The search results were further analysed by the TMTPrePro software package.

    Journal: Data in Brief

    Article Title: Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery

    doi: 10.1016/j.dib.2018.12.041

    Figure Lengend Snippet: Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive Orbitrap mass spectrometer. Raw data generated from the mass spectrometer were processed using Proteome Discover v1.4 and Mascot. The search results were further analysed by the TMTPrePro software package.

    Article Snippet: Raw data files generated by Xcalibur software (Thermo Scientific) were processed using Proteome Discoverer v1.4 (Thermo Scientific) and a local MASCOT server (version 2.3; Matrix Science, London, UK).

    Techniques: Mass Spectrometry, Generated, Software