v splendidus  (ATCC)

Journal: Microorganisms

Article Title: Water Parameters Predicting the Seasonal and Spatial Dynamics of the Vibrio Harveyi- and Splendidus-Clade Pathogens

doi: 10.3390/microorganisms13092167

Figure Lengend Snippet: Copy numbers/g/mL of V. splendidus in Sites 1 ( A ), 2 ( B ), and 3 ( C ). The data for the figure were obtained from samples collected in triplicate from three segments of each site. Nine samples were collected at each site in each sampling month. Each triplicate was taken ~25 m apart to ensure an accurate representation of the site. Average copy numbers were displayed per 1 g of sediment, clams, and oysters and 1 mL of water. Error bars represent standard deviations (N = 9).

Article Snippet: The reference species of V. harveyi (ATCC 14126), V. campbellii (ATCC BAA-1116/BB120), V. splendidus (ATCC 33125), V. parahaemolyticus (ATCC 17802), and V. alginolyticus (ATCC 17749) were used for retesting several primers against each species under the study.

Techniques: Sampling

Journal: bioRxiv

Article Title: Effects of interspecies interactions on marine community ecosystem function

doi: 10.1101/2022.08.26.505414

Figure Lengend Snippet: A) Heatmap of enzymatic activity per OD for various degraders (vertical-axis) in monoculture (first column) or in co-culture with different cross-feeders (horizontal-axis). Numbers indicate total chitinase activity per total OD per µL (Methods). Colours represent activities in co-cultures relative to the degrader monoculture. Yellow colours indicate little to no change compared to the degrader monoculture. Green colours represent a reduced enzymatic activity. Red colours represent an increase in enzyme activity. White panels indicate combinations that were not measured. Stars represent significant changes in growth between the monoculture and respective co-culture: * = p < 0.05, ** = p < 0.01, *** = p < 0.001 (ANOVA). Details about the underlying statistical analysis can be found in the supplementary material (Table S3). B) Enzymatic activity of N- acetylglucosaminidases (yellow), chitobiosidases (green) and endochitinases (red) per yield of V. natriegens monocultures (left) compared to co-cultures with Alteromonas (middle) and Neptunomonas (right). C) Enzymatic activity for V. splendidus monocultures (left) of N- acetylglucosaminidases (yellow), chitobiosidases (green) and endochitinases (red) per yield compared to co-cultures with Alteromonas (middle) and Neptunomonas (right).

Article Snippet: To understand the mechanism by which these two cross-feeders affect chitinase activity, we compared global gene expression profiles of two different degraders in monoculture ( V. splendidus 1A01 and V. natriegens ATCC 14048) with their gene expression profiles in co-culture with one of two cross-feeders ( Alteromonas 4B03 and Neptunomonas 3B05) ( ).

Techniques: Activity Assay, Co-Culture Assay

Journal: bioRxiv

Article Title: Effects of interspecies interactions on marine community ecosystem function

doi: 10.1101/2022.08.26.505414

Figure Lengend Snippet: Degraders show large differences in gene expression profiles in co-cultures with cross-feeders compared to monocultures. Colors indicate log2 fold change (DESeq2) A) Top 100 most significantly differentially expressed genes (regularized logarithm (rlog) function, DESeq2, sorted by adjusted p -value) for V. natriegens monocultures, compared to a co-culture with Alteromonas or Neptunomonas . In total 1,107 out of 4,641 genes were found to be differentially expressed between the mono- and the co-culture with Alteromonas , while 1,585 out of 4,641 genes were differentially expressed in the presence of Neptunomonas compared to the monoculture ( p < 0.05). B) Top 100 most significantly differentially expressed genes (Regularized logarithm (rlog) function, DESeq2, adjusted p value) for V. splendidus monocultures, compared to a co-culture with Alteromonas and Neptunomonas . In total 690 out of 5,237 genes were found to be differentially expressed in between the mono- and the co-culture with Alteromonas , while 1,869 out of 5,237 genes were differentially expressed in the presence of Neptunomonas compared to the monoculture ( p < 0.05).

Article Snippet: To understand the mechanism by which these two cross-feeders affect chitinase activity, we compared global gene expression profiles of two different degraders in monoculture ( V. splendidus 1A01 and V. natriegens ATCC 14048) with their gene expression profiles in co-culture with one of two cross-feeders ( Alteromonas 4B03 and Neptunomonas 3B05) ( ).

Techniques: Gene Expression, Co-Culture Assay

Journal: bioRxiv

Article Title: Effects of interspecies interactions on marine community ecosystem function

doi: 10.1101/2022.08.26.505414

Figure Lengend Snippet: A) Alteromonas induces expression of chitinases in V. splendidus . Differences in chitinase expression in V. splendidus in the presence vs absence of Alteromonas . Dots indicate individual chitinases found in the genome of V. splendidus , black lines indicate significance cut offs (vertical lines indicate |log2 Fold Change| = 0.6, the horizontal line indicates p < 0.05) colours indicate differential expression based on the cut off criteria; down-regulation (red), no difference (yellow), up-regulation (green). Two out of ten chitinases were significantly upregulated in the presence of Alteromonas . B) Neptunomonas induces expression of chitinases in V. splendidus . Differences in chitinase expression in V. splendidus in the presence vs absence of Neptunomonas . Dots indicate individual chitinases found in the genome of V. splendidus , black lines indicate significance cut offs (vertical lines indicate |log2 Fold Change| = 0.6, the horizontal line indicates p < 0.05) colours indicate differential expression based on the cut off criteria; down-regulation (red), no difference (yellow), up-regulation (green). Five out of ten chitinases were significantly upregulated in the presence of Neptunomonas .

Article Snippet: To understand the mechanism by which these two cross-feeders affect chitinase activity, we compared global gene expression profiles of two different degraders in monoculture ( V. splendidus 1A01 and V. natriegens ATCC 14048) with their gene expression profiles in co-culture with one of two cross-feeders ( Alteromonas 4B03 and Neptunomonas 3B05) ( ).

Techniques: Expressing, Quantitative Proteomics