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urea  (Shanghai Macklin Biochemical)
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urea assay  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
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urea nitrogen bun  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
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The effect of ROX in renal function after CI-AKI. (A) Serum concentration of Cr was measured <t>by</t> <t>ELISA;</t> (B) Serum concentration of <t>BUN</t> was measured by ELISA; The data were represented as the mean ± S.D ( N = 10). *** p < 0.001 (CIN vs. Ctrl); # p < 0.05; (ROX vs. CIN); ^ p < 0.05 (ML vs. ROX).
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The effect of ROX in renal function after CI-AKI. (A) Serum concentration of Cr was measured <t>by</t> <t>ELISA;</t> (B) Serum concentration of <t>BUN</t> was measured by ELISA; The data were represented as the mean ± S.D ( N = 10). *** p < 0.001 (CIN vs. Ctrl); # p < 0.05; (ROX vs. CIN); ^ p < 0.05 (ML vs. ROX).
Urea Assay Kit, supplied by Jiancheng Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of ROX in renal function after CI-AKI. (A) Serum concentration of Cr was measured <t>by</t> <t>ELISA;</t> (B) Serum concentration of <t>BUN</t> was measured by ELISA; The data were represented as the mean ± S.D ( N = 10). *** p < 0.001 (CIN vs. Ctrl); # p < 0.05; (ROX vs. CIN); ^ p < 0.05 (ML vs. ROX).
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GDF15 ameliorates renal ectopic lipid deposition and injury in obesity-resistant mice. ( A ) GDF15 protein expression in kidneys ( n = 3). ( B ) Serum GDF15 levels (n = 6). ( C – E ) Quantification of FAT/CD36, PLIN2, and KIM-1 protein expression in kidneys ( n = 3). ( F – H ) Oil Red O, H&E, and PAS staining of renal tissues (Scale bar = 50 μm). ( I ) Serum free fatty acid (FFA) ( n = 6). ( J ) Serum CPT-1 ( n = 6). ( K – M ) Serum levels of KIM-1, blood urea nitrogen <t>(BUN),</t> <t>and</t> <t>creatinine</t> (Cre) ( n = 6). ( N ) Homeostatic model assessment of insulin resistance (HOMA-IR) ( n = 6). Mean ± SD. * p < 0.05 and ** p < 0.01 vs. the NC group; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. the DIO group.
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blood urea nitrogen bun assay kit  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
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GDF15 ameliorates renal ectopic lipid deposition and injury in obesity-resistant mice. ( A ) GDF15 protein expression in kidneys ( n = 3). ( B ) Serum GDF15 levels (n = 6). ( C – E ) Quantification of FAT/CD36, PLIN2, and KIM-1 protein expression in kidneys ( n = 3). ( F – H ) Oil Red O, H&E, and PAS staining of renal tissues (Scale bar = 50 μm). ( I ) Serum free fatty acid (FFA) ( n = 6). ( J ) Serum CPT-1 ( n = 6). ( K – M ) Serum levels of KIM-1, blood urea nitrogen <t>(BUN),</t> <t>and</t> <t>creatinine</t> (Cre) ( n = 6). ( N ) Homeostatic model assessment of insulin resistance (HOMA-IR) ( n = 6). Mean ± SD. * p < 0.05 and ** p < 0.01 vs. the NC group; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. the DIO group.
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urea choline acetate des  (Daihan Scientific)
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GDF15 ameliorates renal ectopic lipid deposition and injury in obesity-resistant mice. ( A ) GDF15 protein expression in kidneys ( n = 3). ( B ) Serum GDF15 levels (n = 6). ( C – E ) Quantification of FAT/CD36, PLIN2, and KIM-1 protein expression in kidneys ( n = 3). ( F – H ) Oil Red O, H&E, and PAS staining of renal tissues (Scale bar = 50 μm). ( I ) Serum free fatty acid (FFA) ( n = 6). ( J ) Serum CPT-1 ( n = 6). ( K – M ) Serum levels of KIM-1, blood urea nitrogen <t>(BUN),</t> <t>and</t> <t>creatinine</t> (Cre) ( n = 6). ( N ) Homeostatic model assessment of insulin resistance (HOMA-IR) ( n = 6). Mean ± SD. * p < 0.05 and ** p < 0.01 vs. the NC group; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. the DIO group.
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The effect of ROX in renal function after CI-AKI. (A) Serum concentration of Cr was measured by ELISA; (B) Serum concentration of BUN was measured by ELISA; The data were represented as the mean ± S.D ( N = 10). *** p < 0.001 (CIN vs. Ctrl); # p < 0.05; (ROX vs. CIN); ^ p < 0.05 (ML vs. ROX).

Journal: Renal Failure

Article Title: Roxadustat attenuates contrast-induced acute kidney injury potentially mediated via the Nrf2/HO-1 signaling pathway

doi: 10.1080/0886022X.2026.2672811

Figure Lengend Snippet: The effect of ROX in renal function after CI-AKI. (A) Serum concentration of Cr was measured by ELISA; (B) Serum concentration of BUN was measured by ELISA; The data were represented as the mean ± S.D ( N = 10). *** p < 0.001 (CIN vs. Ctrl); # p < 0.05; (ROX vs. CIN); ^ p < 0.05 (ML vs. ROX).

Article Snippet: ELISA Kits for blood creatinine (Cr), urea nitrogen (BUN), interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were acquired from Bioengineering Institute of Nanjing Jiancheng (Nanjing, China, C011–2, C013-1, A0101, A0119, A0107).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

GDF15 ameliorates renal ectopic lipid deposition and injury in obesity-resistant mice. ( A ) GDF15 protein expression in kidneys ( n = 3). ( B ) Serum GDF15 levels (n = 6). ( C – E ) Quantification of FAT/CD36, PLIN2, and KIM-1 protein expression in kidneys ( n = 3). ( F – H ) Oil Red O, H&E, and PAS staining of renal tissues (Scale bar = 50 μm). ( I ) Serum free fatty acid (FFA) ( n = 6). ( J ) Serum CPT-1 ( n = 6). ( K – M ) Serum levels of KIM-1, blood urea nitrogen (BUN), and creatinine (Cre) ( n = 6). ( N ) Homeostatic model assessment of insulin resistance (HOMA-IR) ( n = 6). Mean ± SD. * p < 0.05 and ** p < 0.01 vs. the NC group; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. the DIO group.

Journal: Metabolites

Article Title: GDF15 Improves Renal Injury Induced by Ectopic Lipid Deposition via AMPK/SIRT1 Pathway-Mediated Autophagy

doi: 10.3390/metabo16050336

Figure Lengend Snippet: GDF15 ameliorates renal ectopic lipid deposition and injury in obesity-resistant mice. ( A ) GDF15 protein expression in kidneys ( n = 3). ( B ) Serum GDF15 levels (n = 6). ( C – E ) Quantification of FAT/CD36, PLIN2, and KIM-1 protein expression in kidneys ( n = 3). ( F – H ) Oil Red O, H&E, and PAS staining of renal tissues (Scale bar = 50 μm). ( I ) Serum free fatty acid (FFA) ( n = 6). ( J ) Serum CPT-1 ( n = 6). ( K – M ) Serum levels of KIM-1, blood urea nitrogen (BUN), and creatinine (Cre) ( n = 6). ( N ) Homeostatic model assessment of insulin resistance (HOMA-IR) ( n = 6). Mean ± SD. * p < 0.05 and ** p < 0.01 vs. the NC group; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. the DIO group.

Article Snippet: The levels of free fatty acids (Abbkine, Wuhan, China), creatinine (Cre) (Nanjing Jiancheng, Nanjing, China), blood urea nitrogen (BUN) (Nanjing Jiancheng, Nanjing, China), and glucose (Abbkine, Wuhan, China) in serum were measured using assay kits.

Techniques: Expressing, Staining

Blockade of the GDF15-GFRAL pathway induces glucose and lipid metabolic disorders and renal dysfunction in mice. ( A ) Schematic diagram of the animal grouping and treatment protocol. ( B ) GFRAL mRNA expression levels ( n = 3). ( C ) Mouse body size, kidney appearance, kidney weight ( n = 6), and percentage of kidney weight to body weight ( n = 6). ( D , E ) Serum free fatty acids (FFA) and CPT-1 levels ( n = 6). ( F , G ) Serum glucose and insulin levels ( n = 6). ( H ) HOMA-IR ( n = 6). ( I – K ) Serum levels of KIM-1, blood urea nitrogen (BUN), and creatinine (Cre) ( n = 6). Mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the shControl group.

Journal: Metabolites

Article Title: GDF15 Improves Renal Injury Induced by Ectopic Lipid Deposition via AMPK/SIRT1 Pathway-Mediated Autophagy

doi: 10.3390/metabo16050336

Figure Lengend Snippet: Blockade of the GDF15-GFRAL pathway induces glucose and lipid metabolic disorders and renal dysfunction in mice. ( A ) Schematic diagram of the animal grouping and treatment protocol. ( B ) GFRAL mRNA expression levels ( n = 3). ( C ) Mouse body size, kidney appearance, kidney weight ( n = 6), and percentage of kidney weight to body weight ( n = 6). ( D , E ) Serum free fatty acids (FFA) and CPT-1 levels ( n = 6). ( F , G ) Serum glucose and insulin levels ( n = 6). ( H ) HOMA-IR ( n = 6). ( I – K ) Serum levels of KIM-1, blood urea nitrogen (BUN), and creatinine (Cre) ( n = 6). Mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the shControl group.

Article Snippet: The levels of free fatty acids (Abbkine, Wuhan, China), creatinine (Cre) (Nanjing Jiancheng, Nanjing, China), blood urea nitrogen (BUN) (Nanjing Jiancheng, Nanjing, China), and glucose (Abbkine, Wuhan, China) in serum were measured using assay kits.

Techniques: Expressing