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Enzymatics uracil dna glycosylase
Uracil Dna Glycosylase, supplied by Enzymatics, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uracil dna glycosylase/product/Enzymatics
Average 92 stars, based on 8 article reviews
Price from $9.99 to $1999.99
uracil dna glycosylase - by Bioz Stars, 2020-05
92/100 stars

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Selection:

Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci
Article Snippet: .. After end-repair, dA-tailing, ligation to adaptors containing barcode sequences, and size selection using AMPure beads (Agencourt), the synthesized second-strand was digested using uracil DNA glycosylase (Enzymatics). .. A final PCR reaction was performed using Phusion high-fidelity DNA polymerase (NEB).

Ligation:

Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci
Article Snippet: .. After end-repair, dA-tailing, ligation to adaptors containing barcode sequences, and size selection using AMPure beads (Agencourt), the synthesized second-strand was digested using uracil DNA glycosylase (Enzymatics). .. A final PCR reaction was performed using Phusion high-fidelity DNA polymerase (NEB).

Article Title: Transcriptomic Analysis of Petunia hybrida in Response to Salt Stress Using High Throughput RNA Sequencing
Article Snippet: .. Following end-repair (Enzymatics, USA), dA-tailing (Klenow 3′–5′, Enzymatics, USA) and adapter ligation (T4 DNA Ligase HC Enzymatics, USA), the second dUTP-strand was digested by uracil DNA glycosylase (Uracil DNA Glycosylase, Enzymatics, USA). .. The resulting paired-end adaptor ligated-cDNA tags at the 3′ end were amplified using PCR indexed primers (IP) annealing in the adaptor sequence for 15 cycles enriching the final libraries (see for all 6-nt tags/index).

Article Title: Correction: Transcriptome Analysis of Plant Hormone-Related Tomato (Solanum lycopersicum) Genes in a Sunlight-Type Plant Factory
Article Snippet: .. The ligation product was purified and size selected by using AMpure XP (Beckman Coulter) and then the purified second strand DNA was digested using uracil DNA glycosylase (Enzymatics). .. DNA fragments with adapters and an index sequence were amplified using a thermal cycler.

Article Title: GAME9 regulates the biosynthesis of steroidal alkaloids and upstream isoprenoids in the plant mevalonate pathway
Article Snippet: .. After end-repair (Enzymatics), dA-tailing with Klenow 3′-5′ (Enzymatics) and adapter ligation (Quick T4 DNA Ligase, NEB), the dUTP-containing second-strand was digested by uracil DNA glycosylase (Enzymatics). .. The resulting first-strand adaptor-ligated cDNA was used for PCR enrichment (NEBNext High-Fidelity PCR Master Mix, NEB) for 14 cycles.

Synthesized:

Article Title: Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci
Article Snippet: .. After end-repair, dA-tailing, ligation to adaptors containing barcode sequences, and size selection using AMPure beads (Agencourt), the synthesized second-strand was digested using uracil DNA glycosylase (Enzymatics). .. A final PCR reaction was performed using Phusion high-fidelity DNA polymerase (NEB).

Purification:

Article Title: Correction: Transcriptome Analysis of Plant Hormone-Related Tomato (Solanum lycopersicum) Genes in a Sunlight-Type Plant Factory
Article Snippet: .. The ligation product was purified and size selected by using AMpure XP (Beckman Coulter) and then the purified second strand DNA was digested using uracil DNA glycosylase (Enzymatics). .. DNA fragments with adapters and an index sequence were amplified using a thermal cycler.

Incubation:

Article Title: A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq
Article Snippet: .. dUTP excision and amplification of library The size-selected dsDNA product (15 µL) was mixed with 1 µL of uracil DNA glycosylase (Enzymatics, MA) and incubated at 37°C for 30 minutes in a PCR machine. .. Without purification, the mixture was then added to 2 µL of Illumina PE primers (5 µM each) , 6 µL of 5× Phusion HF buffer, 1 µL of 10 mM dNTP, 1 µL of Phusion® Hot Start 2 High-Fidelity DNA Polymerase (NEB, MA) and 4.5 µL of water.

Amplification:

Article Title: A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq
Article Snippet: .. dUTP excision and amplification of library The size-selected dsDNA product (15 µL) was mixed with 1 µL of uracil DNA glycosylase (Enzymatics, MA) and incubated at 37°C for 30 minutes in a PCR machine. .. Without purification, the mixture was then added to 2 µL of Illumina PE primers (5 µM each) , 6 µL of 5× Phusion HF buffer, 1 µL of 10 mM dNTP, 1 µL of Phusion® Hot Start 2 High-Fidelity DNA Polymerase (NEB, MA) and 4.5 µL of water.

Article Title: A novel FADS2 isoform identified in human milk fat globule suppresses FADS2 mediated Δ6-desaturation of omega-3 fatty acids
Article Snippet: .. Followed by uracil DNA glycosylase (Enzymatics, MA) digestion, the adapter-ligated DNA fragments were amplified for 14 PCR cycles. .. The library was purified by Agencourt AMPure XP beads (Beckman Coulter, CA) and quality was checked with an Agilent Bioanalyzer.

Polymerase Chain Reaction:

Article Title: A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq
Article Snippet: .. dUTP excision and amplification of library The size-selected dsDNA product (15 µL) was mixed with 1 µL of uracil DNA glycosylase (Enzymatics, MA) and incubated at 37°C for 30 minutes in a PCR machine. .. Without purification, the mixture was then added to 2 µL of Illumina PE primers (5 µM each) , 6 µL of 5× Phusion HF buffer, 1 µL of 10 mM dNTP, 1 µL of Phusion® Hot Start 2 High-Fidelity DNA Polymerase (NEB, MA) and 4.5 µL of water.

Article Title: A novel FADS2 isoform identified in human milk fat globule suppresses FADS2 mediated Δ6-desaturation of omega-3 fatty acids
Article Snippet: .. Followed by uracil DNA glycosylase (Enzymatics, MA) digestion, the adapter-ligated DNA fragments were amplified for 14 PCR cycles. .. The library was purified by Agencourt AMPure XP beads (Beckman Coulter, CA) and quality was checked with an Agilent Bioanalyzer.

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    Enzymatics uracil dna glycosylase udg
    Uracil Dna Glycosylase Udg, supplied by Enzymatics, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uracil dna glycosylase udg/product/Enzymatics
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    uracil dna glycosylase udg - by Bioz Stars, 2020-05
    91/100 stars
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