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TIB MOLBIOL unmethylated bisulfite treated dna
HeavyMethyl real-time assays for GSTP1 exon and promoter. (A–C) HeavyMethyl GSTP1 exon assay. Amplification plots of 15 ng SssI-methylated <t>DNA</t> indicated by solid or broken lines and 50 ng of unmethylated DNA indicated by lines with circles ( A ). ( B ) 50 ng of unmethylated DNA and 30 pg methylated DNA spiked into 50 ng unmethylated DNA representing a relative sensitivity of 1:1600 ( C ), which were bisulfite treated and subsequently used as template for the HeavyMethyl assay. The LightCycler detection probes were specific for bisulfite-treated unmethylated (B) or methylated DNA (A, C). HeavyMethyl assays performed with and without blocker are indicated by solid and broken lines, respectively. In (A), the HeavyMethyl assay with and without blocker is also indicated by filled and open circles, respectively. ( D ) HeavyMethyl GSTP1 promoter assay. The GSTP1 promoter fragment was amplified on 400 ng unmethylated (five replicates indicated by broken lines), 100 pg SssI-methylated DNA (black diamonds) and mixtures containing 100 pg (six replicates indicated by solid lines) or 50 pg (six replicates indicated by lines labeled with open diamonds) methylated DNA in 400 ng unmethylated DNA. All template DNA samples were bisulfite treated prior to real-time PCR experiments. The products were specifically detected via the FRET signal generated by the Fluorescein- and <t>LCRed640-labeled</t> detection probes specific for methylated bisulfite-treated DNA.
Unmethylated Bisulfite Treated Dna, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/unmethylated bisulfite treated dna/product/TIB MOLBIOL
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
unmethylated bisulfite treated dna - by Bioz Stars, 2020-09
85/100 stars

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1) Product Images from "A real-time PCR assay for DNA-methylation using methylation-specific blockers"

Article Title: A real-time PCR assay for DNA-methylation using methylation-specific blockers

Journal: Nucleic Acids Research

doi: 10.1093/nar/gnh008

HeavyMethyl real-time assays for GSTP1 exon and promoter. (A–C) HeavyMethyl GSTP1 exon assay. Amplification plots of 15 ng SssI-methylated DNA indicated by solid or broken lines and 50 ng of unmethylated DNA indicated by lines with circles ( A ). ( B ) 50 ng of unmethylated DNA and 30 pg methylated DNA spiked into 50 ng unmethylated DNA representing a relative sensitivity of 1:1600 ( C ), which were bisulfite treated and subsequently used as template for the HeavyMethyl assay. The LightCycler detection probes were specific for bisulfite-treated unmethylated (B) or methylated DNA (A, C). HeavyMethyl assays performed with and without blocker are indicated by solid and broken lines, respectively. In (A), the HeavyMethyl assay with and without blocker is also indicated by filled and open circles, respectively. ( D ) HeavyMethyl GSTP1 promoter assay. The GSTP1 promoter fragment was amplified on 400 ng unmethylated (five replicates indicated by broken lines), 100 pg SssI-methylated DNA (black diamonds) and mixtures containing 100 pg (six replicates indicated by solid lines) or 50 pg (six replicates indicated by lines labeled with open diamonds) methylated DNA in 400 ng unmethylated DNA. All template DNA samples were bisulfite treated prior to real-time PCR experiments. The products were specifically detected via the FRET signal generated by the Fluorescein- and LCRed640-labeled detection probes specific for methylated bisulfite-treated DNA.
Figure Legend Snippet: HeavyMethyl real-time assays for GSTP1 exon and promoter. (A–C) HeavyMethyl GSTP1 exon assay. Amplification plots of 15 ng SssI-methylated DNA indicated by solid or broken lines and 50 ng of unmethylated DNA indicated by lines with circles ( A ). ( B ) 50 ng of unmethylated DNA and 30 pg methylated DNA spiked into 50 ng unmethylated DNA representing a relative sensitivity of 1:1600 ( C ), which were bisulfite treated and subsequently used as template for the HeavyMethyl assay. The LightCycler detection probes were specific for bisulfite-treated unmethylated (B) or methylated DNA (A, C). HeavyMethyl assays performed with and without blocker are indicated by solid and broken lines, respectively. In (A), the HeavyMethyl assay with and without blocker is also indicated by filled and open circles, respectively. ( D ) HeavyMethyl GSTP1 promoter assay. The GSTP1 promoter fragment was amplified on 400 ng unmethylated (five replicates indicated by broken lines), 100 pg SssI-methylated DNA (black diamonds) and mixtures containing 100 pg (six replicates indicated by solid lines) or 50 pg (six replicates indicated by lines labeled with open diamonds) methylated DNA in 400 ng unmethylated DNA. All template DNA samples were bisulfite treated prior to real-time PCR experiments. The products were specifically detected via the FRET signal generated by the Fluorescein- and LCRed640-labeled detection probes specific for methylated bisulfite-treated DNA.

Techniques Used: Amplification, Methylation, Promoter Assay, Labeling, Real-time Polymerase Chain Reaction, Generated

Related Articles

Polymerase Chain Reaction:

Article Title: A real-time PCR assay for DNA-methylation using methylation-specific blockers
Article Snippet: .. The 20 µl PCR reactions contained 0.31 µM GSTP1Exon forward primer, 0.31 µM GSTP1Exon reverse primer, 0.25 g/l bovine serum albumin (BSA, Sigma), 0.25 mM dNTPs, 0.25 µM GSTP1Exon anchor probe (GTTTAGAGTTTTTAGTATGGGGTTAATT-Fluo; where Fluo = fluorescein), 0.25 µM GSTP1Exon probe I, specific for methylated bisulfite-treated DNA (LCRed640-TAGTATTAGGTTCGGGTTTTCGG-phos) or probe II, specific for unmethylated bisulfite-treated DNA (LCRed705-TAGTATTAGGTTTGGGTTTTTGG-phos; all probes obtained from TIB-Molbiol), 10 µM GSTP1Exon blocker 3 oligonucleotide ( GTGAGT ATGTGTGGTTTGTGTT-phos), 1× Qiagen reaction buffer, 1.5 mM MgCl2 and 1 U Hotstar polymerase. ..

Methylation:

Article Title: A real-time PCR assay for DNA-methylation using methylation-specific blockers
Article Snippet: .. The 20 µl PCR reactions contained 0.31 µM GSTP1Exon forward primer, 0.31 µM GSTP1Exon reverse primer, 0.25 g/l bovine serum albumin (BSA, Sigma), 0.25 mM dNTPs, 0.25 µM GSTP1Exon anchor probe (GTTTAGAGTTTTTAGTATGGGGTTAATT-Fluo; where Fluo = fluorescein), 0.25 µM GSTP1Exon probe I, specific for methylated bisulfite-treated DNA (LCRed640-TAGTATTAGGTTCGGGTTTTCGG-phos) or probe II, specific for unmethylated bisulfite-treated DNA (LCRed705-TAGTATTAGGTTTGGGTTTTTGG-phos; all probes obtained from TIB-Molbiol), 10 µM GSTP1Exon blocker 3 oligonucleotide ( GTGAGT ATGTGTGGTTTGTGTT-phos), 1× Qiagen reaction buffer, 1.5 mM MgCl2 and 1 U Hotstar polymerase. ..

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    TIB MOLBIOL unmethylated bisulfite treated dna
    HeavyMethyl real-time assays for GSTP1 exon and promoter. (A–C) HeavyMethyl GSTP1 exon assay. Amplification plots of 15 ng SssI-methylated <t>DNA</t> indicated by solid or broken lines and 50 ng of unmethylated DNA indicated by lines with circles ( A ). ( B ) 50 ng of unmethylated DNA and 30 pg methylated DNA spiked into 50 ng unmethylated DNA representing a relative sensitivity of 1:1600 ( C ), which were bisulfite treated and subsequently used as template for the HeavyMethyl assay. The LightCycler detection probes were specific for bisulfite-treated unmethylated (B) or methylated DNA (A, C). HeavyMethyl assays performed with and without blocker are indicated by solid and broken lines, respectively. In (A), the HeavyMethyl assay with and without blocker is also indicated by filled and open circles, respectively. ( D ) HeavyMethyl GSTP1 promoter assay. The GSTP1 promoter fragment was amplified on 400 ng unmethylated (five replicates indicated by broken lines), 100 pg SssI-methylated DNA (black diamonds) and mixtures containing 100 pg (six replicates indicated by solid lines) or 50 pg (six replicates indicated by lines labeled with open diamonds) methylated DNA in 400 ng unmethylated DNA. All template DNA samples were bisulfite treated prior to real-time PCR experiments. The products were specifically detected via the FRET signal generated by the Fluorescein- and <t>LCRed640-labeled</t> detection probes specific for methylated bisulfite-treated DNA.
    Unmethylated Bisulfite Treated Dna, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unmethylated bisulfite treated dna/product/TIB MOLBIOL
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unmethylated bisulfite treated dna - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    HeavyMethyl real-time assays for GSTP1 exon and promoter. (A–C) HeavyMethyl GSTP1 exon assay. Amplification plots of 15 ng SssI-methylated DNA indicated by solid or broken lines and 50 ng of unmethylated DNA indicated by lines with circles ( A ). ( B ) 50 ng of unmethylated DNA and 30 pg methylated DNA spiked into 50 ng unmethylated DNA representing a relative sensitivity of 1:1600 ( C ), which were bisulfite treated and subsequently used as template for the HeavyMethyl assay. The LightCycler detection probes were specific for bisulfite-treated unmethylated (B) or methylated DNA (A, C). HeavyMethyl assays performed with and without blocker are indicated by solid and broken lines, respectively. In (A), the HeavyMethyl assay with and without blocker is also indicated by filled and open circles, respectively. ( D ) HeavyMethyl GSTP1 promoter assay. The GSTP1 promoter fragment was amplified on 400 ng unmethylated (five replicates indicated by broken lines), 100 pg SssI-methylated DNA (black diamonds) and mixtures containing 100 pg (six replicates indicated by solid lines) or 50 pg (six replicates indicated by lines labeled with open diamonds) methylated DNA in 400 ng unmethylated DNA. All template DNA samples were bisulfite treated prior to real-time PCR experiments. The products were specifically detected via the FRET signal generated by the Fluorescein- and LCRed640-labeled detection probes specific for methylated bisulfite-treated DNA.

    Journal: Nucleic Acids Research

    Article Title: A real-time PCR assay for DNA-methylation using methylation-specific blockers

    doi: 10.1093/nar/gnh008

    Figure Lengend Snippet: HeavyMethyl real-time assays for GSTP1 exon and promoter. (A–C) HeavyMethyl GSTP1 exon assay. Amplification plots of 15 ng SssI-methylated DNA indicated by solid or broken lines and 50 ng of unmethylated DNA indicated by lines with circles ( A ). ( B ) 50 ng of unmethylated DNA and 30 pg methylated DNA spiked into 50 ng unmethylated DNA representing a relative sensitivity of 1:1600 ( C ), which were bisulfite treated and subsequently used as template for the HeavyMethyl assay. The LightCycler detection probes were specific for bisulfite-treated unmethylated (B) or methylated DNA (A, C). HeavyMethyl assays performed with and without blocker are indicated by solid and broken lines, respectively. In (A), the HeavyMethyl assay with and without blocker is also indicated by filled and open circles, respectively. ( D ) HeavyMethyl GSTP1 promoter assay. The GSTP1 promoter fragment was amplified on 400 ng unmethylated (five replicates indicated by broken lines), 100 pg SssI-methylated DNA (black diamonds) and mixtures containing 100 pg (six replicates indicated by solid lines) or 50 pg (six replicates indicated by lines labeled with open diamonds) methylated DNA in 400 ng unmethylated DNA. All template DNA samples were bisulfite treated prior to real-time PCR experiments. The products were specifically detected via the FRET signal generated by the Fluorescein- and LCRed640-labeled detection probes specific for methylated bisulfite-treated DNA.

    Article Snippet: The 20 µl PCR reactions contained 0.31 µM GSTP1Exon forward primer, 0.31 µM GSTP1Exon reverse primer, 0.25 g/l bovine serum albumin (BSA, Sigma), 0.25 mM dNTPs, 0.25 µM GSTP1Exon anchor probe (GTTTAGAGTTTTTAGTATGGGGTTAATT-Fluo; where Fluo = fluorescein), 0.25 µM GSTP1Exon probe I, specific for methylated bisulfite-treated DNA (LCRed640-TAGTATTAGGTTCGGGTTTTCGG-phos) or probe II, specific for unmethylated bisulfite-treated DNA (LCRed705-TAGTATTAGGTTTGGGTTTTTGG-phos; all probes obtained from TIB-Molbiol), 10 µM GSTP1Exon blocker 3 oligonucleotide ( GTGAGT ATGTGTGGTTTGTGTT-phos), 1× Qiagen reaction buffer, 1.5 mM MgCl2 and 1 U Hotstar polymerase.

    Techniques: Amplification, Methylation, Promoter Assay, Labeling, Real-time Polymerase Chain Reaction, Generated