unlabeled rabbit monoclonal antibody phospho p38 mapk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc unlabeled rabbit monoclonal antibody phospho p38 mapk
    Calibration of hypotonic lysis of RBCs, fixation of leukocytes and FACS analysis. (A) Leukocyte concentration and viability after hypotonic lysis of erythrocytes, conducted for different time periods. (B) Number of live cells from fishes S1, S2, S3 and S4 following different numbers of washes. First and second wash were used to isolate leukocytes from RBCs; third and fourth wash were used to clean the cells after the use of fixative (Cytofix). Samples were analyzed under the microscope using a hemocytometer. (C–E) Density plots of Hoechst- and PI-stained cells to detect live/dead cells at the different steps of leukocyte isolation. (F–I) FACS analyses of leukocytes after RBC lysis. (F) After a single wash post-lysis and fixation, showing both RBC debris and leukocyte populations. (G) After two post-lysis washes, fixation, and PI gating to obtain only the clear leukocyte population without the RBC debris. (H) Leukocytes were gated and sorted for further analysis by confocal microscopy. (I) Confocal microscopy images of the gated populations (bar = 10 µm). FCS – forward scatter; SSC – side scatter. (J, K) Fixed leukocytes stained with <t>p38</t> antibody; second antibody (2nd Ab) conjugated to Alexa Fluor 488 was used as a control. Data present the analysis of leukocytes from 5 fish. (J) Percent expression of p38 and 2nd Ab. (K) Expression levels of p38 and 2nd Ab in units of geometric mean fluorescence intensity (MFI).
    Unlabeled Rabbit Monoclonal Antibody Phospho P38 Mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unlabeled rabbit monoclonal antibody phospho p38 mapk/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unlabeled rabbit monoclonal antibody phospho p38 mapk - by Bioz Stars, 2024-05
    86/100 stars

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    1) Product Images from "Development and validation of a flow cytometry method to examine circulating leukocyte subpopulations in barramundi ( Lates calcarifer )"

    Article Title: Development and validation of a flow cytometry method to examine circulating leukocyte subpopulations in barramundi ( Lates calcarifer )

    Journal: Comparative Immunology Reports

    doi: 10.1016/j.cirep.2024.200142

    Calibration of hypotonic lysis of RBCs, fixation of leukocytes and FACS analysis. (A) Leukocyte concentration and viability after hypotonic lysis of erythrocytes, conducted for different time periods. (B) Number of live cells from fishes S1, S2, S3 and S4 following different numbers of washes. First and second wash were used to isolate leukocytes from RBCs; third and fourth wash were used to clean the cells after the use of fixative (Cytofix). Samples were analyzed under the microscope using a hemocytometer. (C–E) Density plots of Hoechst- and PI-stained cells to detect live/dead cells at the different steps of leukocyte isolation. (F–I) FACS analyses of leukocytes after RBC lysis. (F) After a single wash post-lysis and fixation, showing both RBC debris and leukocyte populations. (G) After two post-lysis washes, fixation, and PI gating to obtain only the clear leukocyte population without the RBC debris. (H) Leukocytes were gated and sorted for further analysis by confocal microscopy. (I) Confocal microscopy images of the gated populations (bar = 10 µm). FCS – forward scatter; SSC – side scatter. (J, K) Fixed leukocytes stained with p38 antibody; second antibody (2nd Ab) conjugated to Alexa Fluor 488 was used as a control. Data present the analysis of leukocytes from 5 fish. (J) Percent expression of p38 and 2nd Ab. (K) Expression levels of p38 and 2nd Ab in units of geometric mean fluorescence intensity (MFI).
    Figure Legend Snippet: Calibration of hypotonic lysis of RBCs, fixation of leukocytes and FACS analysis. (A) Leukocyte concentration and viability after hypotonic lysis of erythrocytes, conducted for different time periods. (B) Number of live cells from fishes S1, S2, S3 and S4 following different numbers of washes. First and second wash were used to isolate leukocytes from RBCs; third and fourth wash were used to clean the cells after the use of fixative (Cytofix). Samples were analyzed under the microscope using a hemocytometer. (C–E) Density plots of Hoechst- and PI-stained cells to detect live/dead cells at the different steps of leukocyte isolation. (F–I) FACS analyses of leukocytes after RBC lysis. (F) After a single wash post-lysis and fixation, showing both RBC debris and leukocyte populations. (G) After two post-lysis washes, fixation, and PI gating to obtain only the clear leukocyte population without the RBC debris. (H) Leukocytes were gated and sorted for further analysis by confocal microscopy. (I) Confocal microscopy images of the gated populations (bar = 10 µm). FCS – forward scatter; SSC – side scatter. (J, K) Fixed leukocytes stained with p38 antibody; second antibody (2nd Ab) conjugated to Alexa Fluor 488 was used as a control. Data present the analysis of leukocytes from 5 fish. (J) Percent expression of p38 and 2nd Ab. (K) Expression levels of p38 and 2nd Ab in units of geometric mean fluorescence intensity (MFI).

    Techniques Used: Lysis, Concentration Assay, Microscopy, Staining, Isolation, Confocal Microscopy, Expressing, Fluorescence

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    Cell Signaling Technology Inc unlabeled rabbit monoclonal antibody phospho p38 mapk
    Calibration of hypotonic lysis of RBCs, fixation of leukocytes and FACS analysis. (A) Leukocyte concentration and viability after hypotonic lysis of erythrocytes, conducted for different time periods. (B) Number of live cells from fishes S1, S2, S3 and S4 following different numbers of washes. First and second wash were used to isolate leukocytes from RBCs; third and fourth wash were used to clean the cells after the use of fixative (Cytofix). Samples were analyzed under the microscope using a hemocytometer. (C–E) Density plots of Hoechst- and PI-stained cells to detect live/dead cells at the different steps of leukocyte isolation. (F–I) FACS analyses of leukocytes after RBC lysis. (F) After a single wash post-lysis and fixation, showing both RBC debris and leukocyte populations. (G) After two post-lysis washes, fixation, and PI gating to obtain only the clear leukocyte population without the RBC debris. (H) Leukocytes were gated and sorted for further analysis by confocal microscopy. (I) Confocal microscopy images of the gated populations (bar = 10 µm). FCS – forward scatter; SSC – side scatter. (J, K) Fixed leukocytes stained with <t>p38</t> antibody; second antibody (2nd Ab) conjugated to Alexa Fluor 488 was used as a control. Data present the analysis of leukocytes from 5 fish. (J) Percent expression of p38 and 2nd Ab. (K) Expression levels of p38 and 2nd Ab in units of geometric mean fluorescence intensity (MFI).
    Unlabeled Rabbit Monoclonal Antibody Phospho P38 Mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unlabeled rabbit monoclonal antibody phospho p38 mapk/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unlabeled rabbit monoclonal antibody phospho p38 mapk - by Bioz Stars, 2024-05
    86/100 stars
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    Calibration of hypotonic lysis of RBCs, fixation of leukocytes and FACS analysis. (A) Leukocyte concentration and viability after hypotonic lysis of erythrocytes, conducted for different time periods. (B) Number of live cells from fishes S1, S2, S3 and S4 following different numbers of washes. First and second wash were used to isolate leukocytes from RBCs; third and fourth wash were used to clean the cells after the use of fixative (Cytofix). Samples were analyzed under the microscope using a hemocytometer. (C–E) Density plots of Hoechst- and PI-stained cells to detect live/dead cells at the different steps of leukocyte isolation. (F–I) FACS analyses of leukocytes after RBC lysis. (F) After a single wash post-lysis and fixation, showing both RBC debris and leukocyte populations. (G) After two post-lysis washes, fixation, and PI gating to obtain only the clear leukocyte population without the RBC debris. (H) Leukocytes were gated and sorted for further analysis by confocal microscopy. (I) Confocal microscopy images of the gated populations (bar = 10 µm). FCS – forward scatter; SSC – side scatter. (J, K) Fixed leukocytes stained with p38 antibody; second antibody (2nd Ab) conjugated to Alexa Fluor 488 was used as a control. Data present the analysis of leukocytes from 5 fish. (J) Percent expression of p38 and 2nd Ab. (K) Expression levels of p38 and 2nd Ab in units of geometric mean fluorescence intensity (MFI).

    Journal: Comparative Immunology Reports

    Article Title: Development and validation of a flow cytometry method to examine circulating leukocyte subpopulations in barramundi ( Lates calcarifer )

    doi: 10.1016/j.cirep.2024.200142

    Figure Lengend Snippet: Calibration of hypotonic lysis of RBCs, fixation of leukocytes and FACS analysis. (A) Leukocyte concentration and viability after hypotonic lysis of erythrocytes, conducted for different time periods. (B) Number of live cells from fishes S1, S2, S3 and S4 following different numbers of washes. First and second wash were used to isolate leukocytes from RBCs; third and fourth wash were used to clean the cells after the use of fixative (Cytofix). Samples were analyzed under the microscope using a hemocytometer. (C–E) Density plots of Hoechst- and PI-stained cells to detect live/dead cells at the different steps of leukocyte isolation. (F–I) FACS analyses of leukocytes after RBC lysis. (F) After a single wash post-lysis and fixation, showing both RBC debris and leukocyte populations. (G) After two post-lysis washes, fixation, and PI gating to obtain only the clear leukocyte population without the RBC debris. (H) Leukocytes were gated and sorted for further analysis by confocal microscopy. (I) Confocal microscopy images of the gated populations (bar = 10 µm). FCS – forward scatter; SSC – side scatter. (J, K) Fixed leukocytes stained with p38 antibody; second antibody (2nd Ab) conjugated to Alexa Fluor 488 was used as a control. Data present the analysis of leukocytes from 5 fish. (J) Percent expression of p38 and 2nd Ab. (K) Expression levels of p38 and 2nd Ab in units of geometric mean fluorescence intensity (MFI).

    Article Snippet: For p38-staining, cells were incubated with primary unlabeled rabbit monoclonal antibody phospho-p38 MAPK, clone D3F9 (Cell Signaling Technology, USA) diluted 1:200 in BD Perm/Wash buffer and 200 µL was added to each well, followed by incubation at 4 °C for 30 min.

    Techniques: Lysis, Concentration Assay, Microscopy, Staining, Isolation, Confocal Microscopy, Expressing, Fluorescence