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unconjugated mouse anti human complement c9 antibody  (Hycult Biotech)


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    Hycult Biotech unconjugated mouse anti human complement c9 antibody
    Unconjugated Mouse Anti Human Complement C9 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated mouse anti human complement c9 antibody/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unconjugated mouse anti human complement c9 antibody - by Bioz Stars, 2024-10
    92/100 stars

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    unconjugated mouse anti human complement c9 antibody  (Hycult Biotech)
    92
    Hycult Biotech unconjugated mouse anti human complement c9 antibody
    Unconjugated Mouse Anti Human Complement C9 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated mouse anti human complement c9 antibody/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unconjugated mouse anti human complement c9 antibody - by Bioz Stars, 2024-10
    92/100 stars
    		  Buy from Supplier
    unconjugated mouse anti human complement c9 antibody  (Thermo Fisher)
    86
    Thermo Fisher unconjugated mouse anti human complement c9 antibody
    (a) Scheme of the later portion of the complement cascade pathway and downstream signaling. Maroon boxes indicate complement proteins that were positive hits in our glycoproteomic analysis in . (b) Differential α2,6-sialylation of complement C5 and <t>C9</t> in three patient groups. Western blot (anti-C5 and anti-C9) of SNA pulldown samples from pooled patient plasma with corresponding input is shown. Intensity ratios of pulldown/input bands are depicted in the bar plot. (c) Analysis of individual per patient paired ratio of ST6GAL1 to C5 mRNA expression in whole blood of healthy controls (n = 19) or those with seasonal coronavirus (n = 59), or COVID-19 (n = 46), from publicly available dataset Gene Expression Omnibus: GSE161731. Student’s t-test was used to determine p -values. ns: p ≥ 0.05; *: p < 0.05. (d) Representative images of IHC staining against C5b, C9, ST6GAL1 or Pan-Cytokeratin in the lower lobe of the lungs in COVID-19 negative (n = 2) (top) or COVID-19 positive (n = 8) (bottom) autopsy specimens. Scale bars represent 75 μm.
    Unconjugated Mouse Anti Human Complement C9 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated mouse anti human complement c9 antibody/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unconjugated mouse anti human complement c9 antibody - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier

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    (a) Scheme of the later portion of the complement cascade pathway and downstream signaling. Maroon boxes indicate complement proteins that were positive hits in our glycoproteomic analysis in . (b) Differential α2,6-sialylation of complement C5 and C9 in three patient groups. Western blot (anti-C5 and anti-C9) of SNA pulldown samples from pooled patient plasma with corresponding input is shown. Intensity ratios of pulldown/input bands are depicted in the bar plot. (c) Analysis of individual per patient paired ratio of ST6GAL1 to C5 mRNA expression in whole blood of healthy controls (n = 19) or those with seasonal coronavirus (n = 59), or COVID-19 (n = 46), from publicly available dataset Gene Expression Omnibus: GSE161731. Student’s t-test was used to determine p -values. ns: p ≥ 0.05; *: p < 0.05. (d) Representative images of IHC staining against C5b, C9, ST6GAL1 or Pan-Cytokeratin in the lower lobe of the lungs in COVID-19 negative (n = 2) (top) or COVID-19 positive (n = 8) (bottom) autopsy specimens. Scale bars represent 75 μm.

    Journal: medRxiv

    Article Title: α2,6-Sialylation is Upregulated in Severe COVID-19 Implicating the Complement Cascade

    doi: 10.1101/2022.06.06.22275981

    Figure Lengend Snippet: (a) Scheme of the later portion of the complement cascade pathway and downstream signaling. Maroon boxes indicate complement proteins that were positive hits in our glycoproteomic analysis in . (b) Differential α2,6-sialylation of complement C5 and C9 in three patient groups. Western blot (anti-C5 and anti-C9) of SNA pulldown samples from pooled patient plasma with corresponding input is shown. Intensity ratios of pulldown/input bands are depicted in the bar plot. (c) Analysis of individual per patient paired ratio of ST6GAL1 to C5 mRNA expression in whole blood of healthy controls (n = 19) or those with seasonal coronavirus (n = 59), or COVID-19 (n = 46), from publicly available dataset Gene Expression Omnibus: GSE161731. Student’s t-test was used to determine p -values. ns: p ≥ 0.05; *: p < 0.05. (d) Representative images of IHC staining against C5b, C9, ST6GAL1 or Pan-Cytokeratin in the lower lobe of the lungs in COVID-19 negative (n = 2) (top) or COVID-19 positive (n = 8) (bottom) autopsy specimens. Scale bars represent 75 μm.

    Article Snippet: Single and multiplex-chromogenic immunohistochemistry was performed using unconjugated mouse-anti human Interleukin-6, (clone# OTI3G9, Origene, catalog #TA500067, Lot# VE2990982), unconjugated murine anti-human Vimentin (clone# V9, Ventana Medical Systems, Catalog# 790-2917, Lot# E04396, RRID:AB-2335925) and unconjugated mouse anti-human CD-163 clone MRQ-26 (Ventana Medical Systems catalog# 760-4437, lot# V001041, RRID: AB_2335969), unconjugated murine anti human ST6 beta-galactoside alpha-2,6-sialyltransferase 1, (clone# LN1, Thermo Fisher Scientific, Cat# MA5-11900, Lot# XC3519066A, RRID: AB_10980157 ), unconjugated murine anti-human Pan-Cytokeratin (PanK, Thermo Fischer Scientific Cat# MA1-82041, Lot# 985542A RRID: AB_2335731), unconjugated mouse anti-human complement C9 antibody, (clone# X197, Hycult Biotech, catalog# HM2111, Lot# 16152M0714-B, RRID: AB_2067596 ) and unconjugated mouse anti-human terminal complement complex C5b-9, (clone# aEll, Dako Cytomation, catalog# M0777, Lot# 20027911, RRID: AB_2067162 ).

    Techniques: Western Blot, Expressing, Immunohistochemistry