ultraviolet spectroscopy  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ultraviolet spectroscopy
    Ultraviolet Spectroscopy, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultraviolet spectroscopy/product/Thermo Fisher
    Average 99 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    ultraviolet spectroscopy - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. DNA amplification was carried out in a 25 µl PCR reaction containing 20 ng DNA template measured with the NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific), 200 pM forward and reverse primer , 10× PCR buffer, 1.5 mM MgCl2 , 200 µM dNTP and 1 U Platinum Taq polymerase (Invitrogen, Darmstadt, Germany). .. The cycling conditions on a T3000 thermocycler (Biometra, Goettingen, Germany) were as follows: 94°C for 3 minutes; 40 cycles at 94°C for 40 seconds, 62°C for 40 seconds, 72°C for 35 seconds; and hold for 5 minutes at 72°C.

    Mutagenesis:

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. The NanoDrop 2000c spectrophotometer showed the best results for massively parallel sequencing in the mean coverage, but there was only a slight difference in mutation detection. ..

    Next-Generation Sequencing:

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. A previous study by Hadd et al. also used the NanoDrop 2000c spectrophotometer for DNA quantification for targeted next generation sequencing. .. Nevertheless prior to massively parallel sequencing fluorescent dye-based quantification methods are commonly used as they are considered to be the easiest, most reliable and cost effective methods .

    Spectrophotometry:

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. The extracted DNA was quantified by the NanoDrop 2000c spectrophotometer ( ) and the Qubit 2.0 fluorometer ( ). ..

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. A previous study by Hadd et al. also used the NanoDrop 2000c spectrophotometer for DNA quantification for targeted next generation sequencing. .. Nevertheless prior to massively parallel sequencing fluorescent dye-based quantification methods are commonly used as they are considered to be the easiest, most reliable and cost effective methods .

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. The NanoDrop 2000c spectrophotometer showed the best results for massively parallel sequencing in the mean coverage, but there was only a slight difference in mutation detection. ..

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. DNA amplification was carried out in a 25 µl PCR reaction containing 20 ng DNA template measured with the NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific), 200 pM forward and reverse primer , 10× PCR buffer, 1.5 mM MgCl2 , 200 µM dNTP and 1 U Platinum Taq polymerase (Invitrogen, Darmstadt, Germany). .. The cycling conditions on a T3000 thermocycler (Biometra, Goettingen, Germany) were as follows: 94°C for 3 minutes; 40 cycles at 94°C for 40 seconds, 62°C for 40 seconds, 72°C for 35 seconds; and hold for 5 minutes at 72°C.

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. The DNA quantity obtained from the BioRobot M48 was the lowest in 9 samples when measured with the NanoDrop 2000c spectrophotometer and in 8 samples when measured with the Qubit 2.0 fluorometer. .. A 1.3–24.6-fold difference between the concentration of the Maxwell 16 extracts and the extracts of the other systems could be seen.

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. Sedlackova et al. showed that fragmentation does not affect the concentration measured with the NanoDrop 2000c spectrophotometer and that this method might even overestimate the DNA concentration due to the fact that the NanoDrop 2000c spectrophotometer also measures single-stranded DNA and oligonucleotides present. .. Further they state that fluorescent-dye based quantification methods and qPCR are significantly affected by DNA fragmentation: The higher the fragmentation the lower the DNA concentration.

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. Even the NanoDrop 2000c spectrophotometer measurements can be used for subsequent sample analysis with massively parallel sequencing. .. A previous study by Hadd et al. also used the NanoDrop 2000c spectrophotometer for DNA quantification for targeted next generation sequencing.

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. This is in contrast to other studies where the NanoDrop 2000c spectrophotometer always overestimated the DNA concentration . ..

    Sequencing:

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. The NanoDrop 2000c spectrophotometer showed the best results for massively parallel sequencing in the mean coverage, but there was only a slight difference in mutation detection. ..

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. Even the NanoDrop 2000c spectrophotometer measurements can be used for subsequent sample analysis with massively parallel sequencing. .. A previous study by Hadd et al. also used the NanoDrop 2000c spectrophotometer for DNA quantification for targeted next generation sequencing.

    Concentration Assay:

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. Sedlackova et al. showed that fragmentation does not affect the concentration measured with the NanoDrop 2000c spectrophotometer and that this method might even overestimate the DNA concentration due to the fact that the NanoDrop 2000c spectrophotometer also measures single-stranded DNA and oligonucleotides present. .. Further they state that fluorescent-dye based quantification methods and qPCR are significantly affected by DNA fragmentation: The higher the fragmentation the lower the DNA concentration.

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. This is in contrast to other studies where the NanoDrop 2000c spectrophotometer always overestimated the DNA concentration . ..

    Polymerase Chain Reaction:

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
    Article Snippet: .. DNA amplification was carried out in a 25 µl PCR reaction containing 20 ng DNA template measured with the NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific), 200 pM forward and reverse primer , 10× PCR buffer, 1.5 mM MgCl2 , 200 µM dNTP and 1 U Platinum Taq polymerase (Invitrogen, Darmstadt, Germany). .. The cycling conditions on a T3000 thermocycler (Biometra, Goettingen, Germany) were as follows: 94°C for 3 minutes; 40 cycles at 94°C for 40 seconds, 62°C for 40 seconds, 72°C for 35 seconds; and hold for 5 minutes at 72°C.

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    Thermo Fisher agnps
    Preparation procedure of <t>AgNPs,</t> and <t>UV/VIS</t> spectroscopy. ( a ) The reactant before and then the product after the reaction. ( b ) UV/VIS spectroscopy of AgNPs. The maximum absorption peak of ZEO-AgNPs was 415 nm.
    Agnps, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    agnps - by Bioz Stars, 2020-08
    92/100 stars
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    93
    Thermo Fisher 4ap
    (a) Unidirectional freezing of chitosan solution was achieved by creating a uni-axial thermal gradient by exposing the bottom surface of chitosan solution in molds (insulated in Styrofoam, not shown) to a stainless-steel plate submersed in liquid nitrogen. Upon exposure, the uni-axial thermal gradient results in linear formation of ice crystals. (b) A simplified schematic illustrating the process of incorporating <t>4AP</t> drug into Halloysite Nanotubes (HNT) and subsequently mixing with chitosan in a custom mold to produce drug-loaded conduits. A photograph of the prototype is shown along with representative SEM images of the aligned, porous microstructure.
    4ap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    90
    Thermo Fisher uv vis nir spectrometer
    Characterization of <t>CuS@MPG</t> NPs: (A) TEM images and particle size distribution based on TEM results (Inset); (B) HRTEM images; (C) <t>UV-vis-NIR</t> spectra; (D) Photos of CuS@MPG NPs dispersed in water, PBS, fetal bovine serum (FBS), RMPI-1640 culture media containing 10% FBS and DMEM culture media containing 10% FBS.
    Uv Vis Nir Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uv vis nir spectrometer/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    84
    Thermo Fisher rp uhplc uv esi ms
    <t>RP-UHPLC-UV-ESI-MS/MS</t> elution profiles for corn fiber oligomers (CFoligo) incubated with FAEs. CFoligo was incubated with either C1FaeA1 or AnFaeA, and without FAE. (A) Elution profile based on UV (310 nm) showing the release of ferulic acid (FA); p -coumaric acid ( p CA), diferulic acids (diFAs) and triferulic acids (triFAs) from CFoligo after the incubation with FAEs. (B,C) Elution profile of diferulic acids (diFAs) corresponding to the m/z 385 and 401, respectively. The m/z values represent the mass loss of 1 Da (MS operation in negative mode).
    Rp Uhplc Uv Esi Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rp uhplc uv esi ms/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rp uhplc uv esi ms - by Bioz Stars, 2020-08
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    Image Search Results


    Preparation procedure of AgNPs, and UV/VIS spectroscopy. ( a ) The reactant before and then the product after the reaction. ( b ) UV/VIS spectroscopy of AgNPs. The maximum absorption peak of ZEO-AgNPs was 415 nm.

    Journal: Insects

    Article Title: Larvicidal Activity of Synthesized Silver Nanoparticles from Curcuma zedoaria Essential Oil against Culex quinquefasciatus

    doi: 10.3390/insects10010027

    Figure Lengend Snippet: Preparation procedure of AgNPs, and UV/VIS spectroscopy. ( a ) The reactant before and then the product after the reaction. ( b ) UV/VIS spectroscopy of AgNPs. The maximum absorption peak of ZEO-AgNPs was 415 nm.

    Article Snippet: Characterization of Silver Nanoparticles The synthesized AgNPs were identified by Ultraviolet-Visible (UV/VIS) spectroscopy (Varioskan spectral scanning multimode reader, software version 2.4.5 Research Edition, Thermo Scientific, Rockford, IL, USA) at a slit width of 1 nm in the range of 200–600 nm.

    Techniques: UV-Vis Spectroscopy

    (a) Unidirectional freezing of chitosan solution was achieved by creating a uni-axial thermal gradient by exposing the bottom surface of chitosan solution in molds (insulated in Styrofoam, not shown) to a stainless-steel plate submersed in liquid nitrogen. Upon exposure, the uni-axial thermal gradient results in linear formation of ice crystals. (b) A simplified schematic illustrating the process of incorporating 4AP drug into Halloysite Nanotubes (HNT) and subsequently mixing with chitosan in a custom mold to produce drug-loaded conduits. A photograph of the prototype is shown along with representative SEM images of the aligned, porous microstructure.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Aligned Microchannel Polymer-Nanotube Composites for Peripheral Nerve Regeneration: Small Molecule Drug Delivery

    doi: 10.1016/j.jconrel.2019.01.013

    Figure Lengend Snippet: (a) Unidirectional freezing of chitosan solution was achieved by creating a uni-axial thermal gradient by exposing the bottom surface of chitosan solution in molds (insulated in Styrofoam, not shown) to a stainless-steel plate submersed in liquid nitrogen. Upon exposure, the uni-axial thermal gradient results in linear formation of ice crystals. (b) A simplified schematic illustrating the process of incorporating 4AP drug into Halloysite Nanotubes (HNT) and subsequently mixing with chitosan in a custom mold to produce drug-loaded conduits. A photograph of the prototype is shown along with representative SEM images of the aligned, porous microstructure.

    Article Snippet: The concentration of 4AP in the release media was determined by UV-Vis spectroscopy (Genesys 10S, Thermo Fisher Scientific, Waltham, MA, USA) at a λmax of 260 nm using a standard curve previously created for 4AP in PBS [ , , ].

    Techniques:

    TGA spectra (a) before and (b) after loading of 4AP into HNT. The modification of HNT loaded with 4AP (b) is shown as the TGA curve shows decomposition peaks for both 4AP and halloysite. The amount of 4AP loaded into HNT was quantified as mass lost over the given temperature range corresponding to the decomposition of 4AP and is expressed as the percent of total mass of HNT-4AP. (c) Pristine chitosan and composite Cht/HNT-4AP.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Aligned Microchannel Polymer-Nanotube Composites for Peripheral Nerve Regeneration: Small Molecule Drug Delivery

    doi: 10.1016/j.jconrel.2019.01.013

    Figure Lengend Snippet: TGA spectra (a) before and (b) after loading of 4AP into HNT. The modification of HNT loaded with 4AP (b) is shown as the TGA curve shows decomposition peaks for both 4AP and halloysite. The amount of 4AP loaded into HNT was quantified as mass lost over the given temperature range corresponding to the decomposition of 4AP and is expressed as the percent of total mass of HNT-4AP. (c) Pristine chitosan and composite Cht/HNT-4AP.

    Article Snippet: The concentration of 4AP in the release media was determined by UV-Vis spectroscopy (Genesys 10S, Thermo Fisher Scientific, Waltham, MA, USA) at a λmax of 260 nm using a standard curve previously created for 4AP in PBS [ , , ].

    Techniques: Modification

    (a) Degradation of crosslinked composite Cht/HNT-4AP samples in PBS pH 7.4 (black) and PBS with 4mg/mL lysozyme (red) (n=3 samples/timepoint, mean±s.d.). Degradation represented by the weight (%) of polymer remaining. *=p

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Aligned Microchannel Polymer-Nanotube Composites for Peripheral Nerve Regeneration: Small Molecule Drug Delivery

    doi: 10.1016/j.jconrel.2019.01.013

    Figure Lengend Snippet: (a) Degradation of crosslinked composite Cht/HNT-4AP samples in PBS pH 7.4 (black) and PBS with 4mg/mL lysozyme (red) (n=3 samples/timepoint, mean±s.d.). Degradation represented by the weight (%) of polymer remaining. *=p

    Article Snippet: The concentration of 4AP in the release media was determined by UV-Vis spectroscopy (Genesys 10S, Thermo Fisher Scientific, Waltham, MA, USA) at a λmax of 260 nm using a standard curve previously created for 4AP in PBS [ , , ].

    Techniques:

    (a) Lactase dehydrogenase (LDH) leakage cytotoxicity assay testing various doses of 4AP treatment on human Schwann cells in culture. ns=no significance. (b) MTS assay of human Schwann cells cultured on TCPS with no drug (control) or 1, 5, and 10 µg/mL of 4AP for up to 21 days. *=p

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Aligned Microchannel Polymer-Nanotube Composites for Peripheral Nerve Regeneration: Small Molecule Drug Delivery

    doi: 10.1016/j.jconrel.2019.01.013

    Figure Lengend Snippet: (a) Lactase dehydrogenase (LDH) leakage cytotoxicity assay testing various doses of 4AP treatment on human Schwann cells in culture. ns=no significance. (b) MTS assay of human Schwann cells cultured on TCPS with no drug (control) or 1, 5, and 10 µg/mL of 4AP for up to 21 days. *=p

    Article Snippet: The concentration of 4AP in the release media was determined by UV-Vis spectroscopy (Genesys 10S, Thermo Fisher Scientific, Waltham, MA, USA) at a λmax of 260 nm using a standard curve previously created for 4AP in PBS [ , , ].

    Techniques: Cytotoxicity Assay, MTS Assay, Cell Culture

    (a) In vitro immunofluorescent staining images showing human Schwann cells stained for NGF, P0, and BDNF (all stained red, respectively by row) in a dose response study to 4AP drug treatment. Control groups, which received media without drug, were compared to 1, 5, and 10 µg/mL drug media solutions, respectively by column. SB=10µm. (b) Quantification of immunofluorescent staining of NGF, P0, and BDNF (left to right, respectively). The intensity of staining obtained with each antibody was measured and displayed as box-plots with 5 to 95% confidence intervals (n=5 images/group). *=p

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Aligned Microchannel Polymer-Nanotube Composites for Peripheral Nerve Regeneration: Small Molecule Drug Delivery

    doi: 10.1016/j.jconrel.2019.01.013

    Figure Lengend Snippet: (a) In vitro immunofluorescent staining images showing human Schwann cells stained for NGF, P0, and BDNF (all stained red, respectively by row) in a dose response study to 4AP drug treatment. Control groups, which received media without drug, were compared to 1, 5, and 10 µg/mL drug media solutions, respectively by column. SB=10µm. (b) Quantification of immunofluorescent staining of NGF, P0, and BDNF (left to right, respectively). The intensity of staining obtained with each antibody was measured and displayed as box-plots with 5 to 95% confidence intervals (n=5 images/group). *=p

    Article Snippet: The concentration of 4AP in the release media was determined by UV-Vis spectroscopy (Genesys 10S, Thermo Fisher Scientific, Waltham, MA, USA) at a λmax of 260 nm using a standard curve previously created for 4AP in PBS [ , , ].

    Techniques: In Vitro, Staining

    Characterization of CuS@MPG NPs: (A) TEM images and particle size distribution based on TEM results (Inset); (B) HRTEM images; (C) UV-vis-NIR spectra; (D) Photos of CuS@MPG NPs dispersed in water, PBS, fetal bovine serum (FBS), RMPI-1640 culture media containing 10% FBS and DMEM culture media containing 10% FBS.

    Journal: Theranostics

    Article Title: Manganese (II) Chelate Functionalized Copper Sulfide Nanoparticles for Efficient Magnetic Resonance/Photoacoustic Dual-Modal Imaging Guided Photothermal Therapy

    doi: 10.7150/thno.11754

    Figure Lengend Snippet: Characterization of CuS@MPG NPs: (A) TEM images and particle size distribution based on TEM results (Inset); (B) HRTEM images; (C) UV-vis-NIR spectra; (D) Photos of CuS@MPG NPs dispersed in water, PBS, fetal bovine serum (FBS), RMPI-1640 culture media containing 10% FBS and DMEM culture media containing 10% FBS.

    Article Snippet: The absorption spectra of CuS@MPG NPs were recorded using an UV-vis-NIR spectrometer (Evolution 220, Thermo Scientific) using quartz cuvettes with an optical path of 1 cm.

    Techniques: Transmission Electron Microscopy

    RP-UHPLC-UV-ESI-MS/MS elution profiles for corn fiber oligomers (CFoligo) incubated with FAEs. CFoligo was incubated with either C1FaeA1 or AnFaeA, and without FAE. (A) Elution profile based on UV (310 nm) showing the release of ferulic acid (FA); p -coumaric acid ( p CA), diferulic acids (diFAs) and triferulic acids (triFAs) from CFoligo after the incubation with FAEs. (B,C) Elution profile of diferulic acids (diFAs) corresponding to the m/z 385 and 401, respectively. The m/z values represent the mass loss of 1 Da (MS operation in negative mode).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Feruloyl Esterases for Biorefineries: Subfamily Classified Specificity for Natural Substrates

    doi: 10.3389/fbioe.2020.00332

    Figure Lengend Snippet: RP-UHPLC-UV-ESI-MS/MS elution profiles for corn fiber oligomers (CFoligo) incubated with FAEs. CFoligo was incubated with either C1FaeA1 or AnFaeA, and without FAE. (A) Elution profile based on UV (310 nm) showing the release of ferulic acid (FA); p -coumaric acid ( p CA), diferulic acids (diFAs) and triferulic acids (triFAs) from CFoligo after the incubation with FAEs. (B,C) Elution profile of diferulic acids (diFAs) corresponding to the m/z 385 and 401, respectively. The m/z values represent the mass loss of 1 Da (MS operation in negative mode).

    Article Snippet: RP-UHPLC-UV-ESI-MS/MS Analysis: Model Substrates Methyl Ferulate and Methyl p -Coumarate Incubated With FAEs All samples were analyzed on an Accela reversed phase ultra-high performance liquid chromatography (RP-UHPLC) system, equipped with a pump, degasser, autosampler, and photodiode array (PDA) detector (Thermo Scientific, San Jose, CA, United States).

    Techniques: Tandem Mass Spectroscopy, Incubation

    Heatmap presenting the specificity of FAEs. This heatmap highlights the release of diFAs and triFAs by 14 FAEs (representing 6 SFs) from the PCW-derived and natural substrates corn fiber oligomers (CFoligo), corn stover (CS), wheat straw (WS), and corn stover lignin isolate (CSlignin). All substrates were incubated with FAEs and without (broth) at 37°C for 19 h in duplicate. The results are given as percentages of bound content as measured by RP-UHPLC-UV-ESI-MS/MS ( n = 2). The broth is the culture supernatant of P. pastoris which was grown without FAE insertion (negative control).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Feruloyl Esterases for Biorefineries: Subfamily Classified Specificity for Natural Substrates

    doi: 10.3389/fbioe.2020.00332

    Figure Lengend Snippet: Heatmap presenting the specificity of FAEs. This heatmap highlights the release of diFAs and triFAs by 14 FAEs (representing 6 SFs) from the PCW-derived and natural substrates corn fiber oligomers (CFoligo), corn stover (CS), wheat straw (WS), and corn stover lignin isolate (CSlignin). All substrates were incubated with FAEs and without (broth) at 37°C for 19 h in duplicate. The results are given as percentages of bound content as measured by RP-UHPLC-UV-ESI-MS/MS ( n = 2). The broth is the culture supernatant of P. pastoris which was grown without FAE insertion (negative control).

    Article Snippet: RP-UHPLC-UV-ESI-MS/MS Analysis: Model Substrates Methyl Ferulate and Methyl p -Coumarate Incubated With FAEs All samples were analyzed on an Accela reversed phase ultra-high performance liquid chromatography (RP-UHPLC) system, equipped with a pump, degasser, autosampler, and photodiode array (PDA) detector (Thermo Scientific, San Jose, CA, United States).

    Techniques: Derivative Assay, Incubation, Tandem Mass Spectroscopy, Negative Control

    The release of the diFA corresponding to a m/z value of 401 10.66 min . The release of this diFA (μg of compound/mg dry matter of natural substrate) from corn fiber oligomers (CFoligo), corn stover (CS), wheat straw (WS), and corn stover lignin isolate (CSlignin) that were incubated with and without (broth) FAEs at 37°C for 19 h. Samples were measured by RP-UHPLC-UV-ESI-MS/MS ( n = 2). The broth is the culture supernatant of P. pastoris which was grown without FAE insertion (negative control). Error bars represent the average standard deviation based on determined absolute numbers.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Feruloyl Esterases for Biorefineries: Subfamily Classified Specificity for Natural Substrates

    doi: 10.3389/fbioe.2020.00332

    Figure Lengend Snippet: The release of the diFA corresponding to a m/z value of 401 10.66 min . The release of this diFA (μg of compound/mg dry matter of natural substrate) from corn fiber oligomers (CFoligo), corn stover (CS), wheat straw (WS), and corn stover lignin isolate (CSlignin) that were incubated with and without (broth) FAEs at 37°C for 19 h. Samples were measured by RP-UHPLC-UV-ESI-MS/MS ( n = 2). The broth is the culture supernatant of P. pastoris which was grown without FAE insertion (negative control). Error bars represent the average standard deviation based on determined absolute numbers.

    Article Snippet: RP-UHPLC-UV-ESI-MS/MS Analysis: Model Substrates Methyl Ferulate and Methyl p -Coumarate Incubated With FAEs All samples were analyzed on an Accela reversed phase ultra-high performance liquid chromatography (RP-UHPLC) system, equipped with a pump, degasser, autosampler, and photodiode array (PDA) detector (Thermo Scientific, San Jose, CA, United States).

    Techniques: Incubation, Tandem Mass Spectroscopy, Negative Control, Standard Deviation