ultraviolet spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ultraviolet spectrometer
    Ultraviolet Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultraviolet spectrometer/product/Thermo Fisher
    Average 76 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultraviolet spectrometer - by Bioz Stars, 2020-03
    76/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Hyperglycemia acts in synergy with hypoxia to maintain the pro-inflammatory phenotype of macrophages
    Article Snippet: Paragraph title: 2.3 RNA extraction and TNF-alpha gene expression ... Total RNA quantity and purity were determined using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, NanoDrop Technologies).

    Amplification:

    Article Title: Effect of miR-503 Down-Regulation on Growth and Invasion of Esophagus Carcinoma and Related Immune Function
    Article Snippet: After measuring concentration and purity by ultraviolet spectrometer, total RNA was used as the template for in vitro reverse-transcription using a test kit (Invitrogen, US) to synthesize cDNA. .. Semi-quantification of target gene amplification was performed by 2−ΔCt method.

    Agarose Gel Electrophoresis:

    Article Title: Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease
    Article Snippet: The RNA samples were analyzed for OD260, OD280 and OD230 using an ultraviolet spectrometer (Applied Biosystems, Foster City, CA, USA) to determine RNA purity (OD260/280 > 1.8 and OD260/230 > 1.5). .. Formaldehyde denaturing agarose gel electrophoresis was employed to determine RNA integrity through examination of the 28S to 18S ratio in the RNA samples.

    In Vitro:

    Article Title: Effect of miR-503 Down-Regulation on Growth and Invasion of Esophagus Carcinoma and Related Immune Function
    Article Snippet: .. After measuring concentration and purity by ultraviolet spectrometer, total RNA was used as the template for in vitro reverse-transcription using a test kit (Invitrogen, US) to synthesize cDNA. .. Real-time PCR was then used to amplify the target gene using specific primers ( , Sangon, China) under the following condition: 55°C for 1 min, followed by 35 cycles each with 92°C denaturing for 30 s, 58°C annealing for 45 s, and 72°C elongation for 35 s. Using GAPDH as the internal reference, fluorescent signals were quantified to determine CT values of all samples and standards.

    Modification:

    Article Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
    Article Snippet: Gene expression (RT-qPCR) analysis Total RNA was extracted using a modified Trizol isolation method at the given time points. .. The purity and quantity of total RNA were evaluated using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, Thermo Scientific, Ireland).

    Concentration Assay:

    Article Title: Effect of miR-503 Down-Regulation on Growth and Invasion of Esophagus Carcinoma and Related Immune Function
    Article Snippet: .. After measuring concentration and purity by ultraviolet spectrometer, total RNA was used as the template for in vitro reverse-transcription using a test kit (Invitrogen, US) to synthesize cDNA. .. Real-time PCR was then used to amplify the target gene using specific primers ( , Sangon, China) under the following condition: 55°C for 1 min, followed by 35 cycles each with 92°C denaturing for 30 s, 58°C annealing for 45 s, and 72°C elongation for 35 s. Using GAPDH as the internal reference, fluorescent signals were quantified to determine CT values of all samples and standards.

    Isolation:

    Article Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
    Article Snippet: Gene expression (RT-qPCR) analysis Total RNA was extracted using a modified Trizol isolation method at the given time points. .. The purity and quantity of total RNA were evaluated using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, Thermo Scientific, Ireland).

    Article Title: Hyperglycemia acts in synergy with hypoxia to maintain the pro-inflammatory phenotype of macrophages
    Article Snippet: 2.3 RNA extraction and TNF-alpha gene expression Total RNAs were extracted from macrophages after 1 hour or 17 hours post LPS activation using a variant of Trizol isolation. .. Total RNA quantity and purity were determined using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, NanoDrop Technologies).

    Spectrophotometry:

    Article Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
    Article Snippet: .. The purity and quantity of total RNA were evaluated using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, Thermo Scientific, Ireland). .. Reverse transcription (RT) was performed using MJ Research PTC-200 DNA Engine system according to the manufacturer's protocol (Promega RT System, UK).

    Article Title: Hyperglycemia acts in synergy with hypoxia to maintain the pro-inflammatory phenotype of macrophages
    Article Snippet: .. Total RNA quantity and purity were determined using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, NanoDrop Technologies). .. RNA integrity was checked electrophoretically using the RNA 6000 Nano LabChip kit on Agilent Bioanalyser 2100 (Agilent Technologies).

    Purification:

    Article Title: Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease
    Article Snippet: Total RNA was extracted from the samples using a human tissue RNA purification kit (Norgen Biotek, Thorold, ON, USA) according to the manufacturer’s instructions. .. The RNA samples were analyzed for OD260, OD280 and OD230 using an ultraviolet spectrometer (Applied Biosystems, Foster City, CA, USA) to determine RNA purity (OD260/280 > 1.8 and OD260/230 > 1.5).

    Article Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
    Article Snippet: Phase separation was conducted with chloroform and the total RNA contained in the aqueous phase was purified using RNeasy® mini kit column (QIAGEN, Hilden, Germany), according to the supplier's protocol. .. The purity and quantity of total RNA were evaluated using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, Thermo Scientific, Ireland).

    Article Title: Hyperglycemia acts in synergy with hypoxia to maintain the pro-inflammatory phenotype of macrophages
    Article Snippet: Phase separation was performed by adding chloroform (Sigma-Aldrich), and total RNA was purified using an RNeasy kit (Qiagen), according to the supplier’s recommended procedure. .. Total RNA quantity and purity were determined using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, NanoDrop Technologies).

    Real-time Polymerase Chain Reaction:

    Article Title: Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease
    Article Snippet: The RNA samples were analyzed for OD260, OD280 and OD230 using an ultraviolet spectrometer (Applied Biosystems, Foster City, CA, USA) to determine RNA purity (OD260/280 > 1.8 and OD260/230 > 1.5). .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis was performed to determine the relative expression levels of CCR7+ gene transcripts in the COPD and control tissues.

    Article Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
    Article Snippet: The purity and quantity of total RNA were evaluated using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, Thermo Scientific, Ireland). .. The prepared cDNA was monitored using SYBRGreen master mix (QIAGEN, Hilden, Germany) by real-time PCR using StepOnePlus™ Real-Time PCR System (Applied Bioscience, Switzerland).

    Article Title: Effect of miR-503 Down-Regulation on Growth and Invasion of Esophagus Carcinoma and Related Immune Function
    Article Snippet: Paragraph title: Real-time PCR ... After measuring concentration and purity by ultraviolet spectrometer, total RNA was used as the template for in vitro reverse-transcription using a test kit (Invitrogen, US) to synthesize cDNA.

    Activation Assay:

    Article Title: Hyperglycemia acts in synergy with hypoxia to maintain the pro-inflammatory phenotype of macrophages
    Article Snippet: 2.3 RNA extraction and TNF-alpha gene expression Total RNAs were extracted from macrophages after 1 hour or 17 hours post LPS activation using a variant of Trizol isolation. .. Total RNA quantity and purity were determined using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, NanoDrop Technologies).

    Quantitative RT-PCR:

    Article Title: Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease
    Article Snippet: The RNA samples were analyzed for OD260, OD280 and OD230 using an ultraviolet spectrometer (Applied Biosystems, Foster City, CA, USA) to determine RNA purity (OD260/280 > 1.8 and OD260/230 > 1.5). .. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis was performed to determine the relative expression levels of CCR7+ gene transcripts in the COPD and control tissues.

    Article Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
    Article Snippet: Paragraph title: Gene expression (RT-qPCR) analysis ... The purity and quantity of total RNA were evaluated using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, Thermo Scientific, Ireland).

    Expressing:

    Article Title: Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease
    Article Snippet: Paragraph title: Determination of CD83+ , CD1a+ and CCR7+ gene expression levels in COPD and control tissues ... The RNA samples were analyzed for OD260, OD280 and OD230 using an ultraviolet spectrometer (Applied Biosystems, Foster City, CA, USA) to determine RNA purity (OD260/280 > 1.8 and OD260/230 > 1.5).

    Article Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
    Article Snippet: Paragraph title: Gene expression (RT-qPCR) analysis ... The purity and quantity of total RNA were evaluated using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, Thermo Scientific, Ireland).

    Article Title: Hyperglycemia acts in synergy with hypoxia to maintain the pro-inflammatory phenotype of macrophages
    Article Snippet: Paragraph title: 2.3 RNA extraction and TNF-alpha gene expression ... Total RNA quantity and purity were determined using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, NanoDrop Technologies).

    Polymerase Chain Reaction:

    Article Title: Hyperglycemia acts in synergy with hypoxia to maintain the pro-inflammatory phenotype of macrophages
    Article Snippet: Total RNA quantity and purity were determined using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, NanoDrop Technologies). .. To control the macrophage activation by LPS, gene transcription of Tumor Necrosis Factor Alpha (TNF-Alpha) was examined using real-time RT-polymerase chain reaction (PCR).

    Variant Assay:

    Article Title: Hyperglycemia acts in synergy with hypoxia to maintain the pro-inflammatory phenotype of macrophages
    Article Snippet: 2.3 RNA extraction and TNF-alpha gene expression Total RNAs were extracted from macrophages after 1 hour or 17 hours post LPS activation using a variant of Trizol isolation. .. Total RNA quantity and purity were determined using an ultraviolet spectrometer (NanoDrop ND-1000 Spectrophotometer, NanoDrop Technologies).

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    Thermo Fisher uv vis nir spectrometer
    Characterization of <t>CuS@MPG</t> NPs: (A) TEM images and particle size distribution based on TEM results (Inset); (B) HRTEM images; (C) <t>UV-vis-NIR</t> spectra; (D) Photos of CuS@MPG NPs dispersed in water, PBS, fetal bovine serum (FBS), RMPI-1640 culture media containing 10% FBS and DMEM culture media containing 10% FBS.
    Uv Vis Nir Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uv vis nir spectrometer/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    uv vis nir spectrometer - by Bioz Stars, 2020-03
    80/100 stars
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    82
    Thermo Fisher uv vis spectroscopy transmission spectra
    <t>UV–vis</t> <t>transmission</t> <t>spectra</t> of CNC and CNC/lignin films with various lignin concentrations.
    Uv Vis Spectroscopy Transmission Spectra, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uv vis spectroscopy transmission spectra/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    uv vis spectroscopy transmission spectra - by Bioz Stars, 2020-03
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    99
    Thermo Fisher uv vis spectrometer
    Characterization of the materials used in the study i.e.: Representative SEM images obtained for a Ti 2 AlN starting MAX phase, b Ti 2 N MXene after HF etching, and c <t>2D</t> sheets of Ti 2 NT x MXene after delamination; d Cross-sectional HREM image, e FFT image together with related f IFFT extraction of multilayered structure obtained for 2D sheets of Ti 2 NT x MXene; g The intensity pattern revealing a period related to d-spacing of 0.97 nm; h SADP electron diffraction with indication of the characteristic rings for 2D Ti-N layers; i Comparison of the X-ray diffraction patterns obtained for: Ti 2 AlN MAX phase and resulting Ti 2 NT x MXene; j EDX analysis, k <t>UV–VIS</t> spectrum accompanied by Tyndall test, as well as l FTIR spectrum of the Ti 2 NT x MXene after delamination
    Uv Vis Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uv vis spectrometer/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    uv vis spectrometer - by Bioz Stars, 2020-03
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    83
    Thermo Fisher ultraviolet visible spectroscopy uv vis
    Characterization of Cap-AuNPs. Notes: ( A ) SDS-PAGE (left) and western blot (right) analysis of Cap protein expressed in E. coli BL21 (DE3). SDS-PAGE gel was stained with silver nitrate. Lane M: molecular weight markers; Lane 1: extract of BL21 cells transformed with pET28a-Cap plasmid induced by IPTG; Lane 2: purified Cap protein. ( B ) <t>UV-vis</t> spectrum of AuNPs (λ max =522 nm) and Cap-AuNPs (λ max =529 nm). ( C ) Transmission electron micrograph of AuNPs. Scale bars =50 nm. ( D ) Transmission electron micrograph of Cap-AuNPs. Scale bars =50 nm. ( E ) DLS data showing size distribution of AuNPs, Cap-AuNPs, and lyophilized Cap-AuNPs. ( F ) Zeta potential of AuNPs, Cap-AuNPs, and lyophilized Cap-AuNPs (n=3 per group). ( G ) FTIR spectra of Cap and Cap-AuNPs. The S-H bonding in Cap is identified by a circle with a weak peak near 2,550 cm −1 region. Abbreviations: Cap, capsid; AuNPs, gold nanoparticles; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; E. coli , Escherichia coli ; IPTG, isopropyl-β-d-thiogalactoside; UV-vis, <t>ultraviolet-visible</t> <t>spectroscopy;</t> DLS, dynamic light scattering; FTIR, Fourier transform infrared spectroscopy.
    Ultraviolet Visible Spectroscopy Uv Vis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultraviolet visible spectroscopy uv vis/product/Thermo Fisher
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Characterization of CuS@MPG NPs: (A) TEM images and particle size distribution based on TEM results (Inset); (B) HRTEM images; (C) UV-vis-NIR spectra; (D) Photos of CuS@MPG NPs dispersed in water, PBS, fetal bovine serum (FBS), RMPI-1640 culture media containing 10% FBS and DMEM culture media containing 10% FBS.

    Journal: Theranostics

    Article Title: Manganese (II) Chelate Functionalized Copper Sulfide Nanoparticles for Efficient Magnetic Resonance/Photoacoustic Dual-Modal Imaging Guided Photothermal Therapy

    doi: 10.7150/thno.11754

    Figure Lengend Snippet: Characterization of CuS@MPG NPs: (A) TEM images and particle size distribution based on TEM results (Inset); (B) HRTEM images; (C) UV-vis-NIR spectra; (D) Photos of CuS@MPG NPs dispersed in water, PBS, fetal bovine serum (FBS), RMPI-1640 culture media containing 10% FBS and DMEM culture media containing 10% FBS.

    Article Snippet: The absorption spectra of CuS@MPG NPs were recorded using an UV-vis-NIR spectrometer (Evolution 220, Thermo Scientific) using quartz cuvettes with an optical path of 1 cm.

    Techniques: Transmission Electron Microscopy

    UV–vis transmission spectra of CNC and CNC/lignin films with various lignin concentrations.

    Journal: ACS Omega

    Article Title: Transparent and Homogenous Cellulose Nanocrystal/Lignin UV-Protection Films

    doi: 10.1021/acsomega.8b01345

    Figure Lengend Snippet: UV–vis transmission spectra of CNC and CNC/lignin films with various lignin concentrations.

    Article Snippet: UV–Vis Spectroscopy Transmission spectra of the aqueous lignin solutions and CNC/lignin films were carried out using a Thermo Scientific GENESYS 10S UV–vis spectrometer in the wavelength range of 200–800 nm.

    Techniques: Transmission Assay

    Characterization of the materials used in the study i.e.: Representative SEM images obtained for a Ti 2 AlN starting MAX phase, b Ti 2 N MXene after HF etching, and c 2D sheets of Ti 2 NT x MXene after delamination; d Cross-sectional HREM image, e FFT image together with related f IFFT extraction of multilayered structure obtained for 2D sheets of Ti 2 NT x MXene; g The intensity pattern revealing a period related to d-spacing of 0.97 nm; h SADP electron diffraction with indication of the characteristic rings for 2D Ti-N layers; i Comparison of the X-ray diffraction patterns obtained for: Ti 2 AlN MAX phase and resulting Ti 2 NT x MXene; j EDX analysis, k UV–VIS spectrum accompanied by Tyndall test, as well as l FTIR spectrum of the Ti 2 NT x MXene after delamination

    Journal: Journal of Nanobiotechnology

    Article Title: Multilayered stable 2D nano-sheets of Ti2NTx MXene: synthesis, characterization, and anticancer activity

    doi: 10.1186/s12951-019-0545-4

    Figure Lengend Snippet: Characterization of the materials used in the study i.e.: Representative SEM images obtained for a Ti 2 AlN starting MAX phase, b Ti 2 N MXene after HF etching, and c 2D sheets of Ti 2 NT x MXene after delamination; d Cross-sectional HREM image, e FFT image together with related f IFFT extraction of multilayered structure obtained for 2D sheets of Ti 2 NT x MXene; g The intensity pattern revealing a period related to d-spacing of 0.97 nm; h SADP electron diffraction with indication of the characteristic rings for 2D Ti-N layers; i Comparison of the X-ray diffraction patterns obtained for: Ti 2 AlN MAX phase and resulting Ti 2 NT x MXene; j EDX analysis, k UV–VIS spectrum accompanied by Tyndall test, as well as l FTIR spectrum of the Ti 2 NT x MXene after delamination

    Article Snippet: The transmittance spectrum of the multilayered 2D sheets of Ti2 NTx was obtained using a UV–VIS spectrometer (Evolution 220 from Thermo Scientific) equipped with an integrating sphere (0.3 s of integration), in a range from 220 to 1100 nm, with a resolution of 1 nm, and a scanning speed of 200 nm min−1 .

    Techniques:

    Characterization of Cap-AuNPs. Notes: ( A ) SDS-PAGE (left) and western blot (right) analysis of Cap protein expressed in E. coli BL21 (DE3). SDS-PAGE gel was stained with silver nitrate. Lane M: molecular weight markers; Lane 1: extract of BL21 cells transformed with pET28a-Cap plasmid induced by IPTG; Lane 2: purified Cap protein. ( B ) UV-vis spectrum of AuNPs (λ max =522 nm) and Cap-AuNPs (λ max =529 nm). ( C ) Transmission electron micrograph of AuNPs. Scale bars =50 nm. ( D ) Transmission electron micrograph of Cap-AuNPs. Scale bars =50 nm. ( E ) DLS data showing size distribution of AuNPs, Cap-AuNPs, and lyophilized Cap-AuNPs. ( F ) Zeta potential of AuNPs, Cap-AuNPs, and lyophilized Cap-AuNPs (n=3 per group). ( G ) FTIR spectra of Cap and Cap-AuNPs. The S-H bonding in Cap is identified by a circle with a weak peak near 2,550 cm −1 region. Abbreviations: Cap, capsid; AuNPs, gold nanoparticles; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; E. coli , Escherichia coli ; IPTG, isopropyl-β-d-thiogalactoside; UV-vis, ultraviolet-visible spectroscopy; DLS, dynamic light scattering; FTIR, Fourier transform infrared spectroscopy.

    Journal: International Journal of Nanomedicine

    Article Title: Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2

    doi: 10.2147/IJN.S140789

    Figure Lengend Snippet: Characterization of Cap-AuNPs. Notes: ( A ) SDS-PAGE (left) and western blot (right) analysis of Cap protein expressed in E. coli BL21 (DE3). SDS-PAGE gel was stained with silver nitrate. Lane M: molecular weight markers; Lane 1: extract of BL21 cells transformed with pET28a-Cap plasmid induced by IPTG; Lane 2: purified Cap protein. ( B ) UV-vis spectrum of AuNPs (λ max =522 nm) and Cap-AuNPs (λ max =529 nm). ( C ) Transmission electron micrograph of AuNPs. Scale bars =50 nm. ( D ) Transmission electron micrograph of Cap-AuNPs. Scale bars =50 nm. ( E ) DLS data showing size distribution of AuNPs, Cap-AuNPs, and lyophilized Cap-AuNPs. ( F ) Zeta potential of AuNPs, Cap-AuNPs, and lyophilized Cap-AuNPs (n=3 per group). ( G ) FTIR spectra of Cap and Cap-AuNPs. The S-H bonding in Cap is identified by a circle with a weak peak near 2,550 cm −1 region. Abbreviations: Cap, capsid; AuNPs, gold nanoparticles; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; E. coli , Escherichia coli ; IPTG, isopropyl-β-d-thiogalactoside; UV-vis, ultraviolet-visible spectroscopy; DLS, dynamic light scattering; FTIR, Fourier transform infrared spectroscopy.

    Article Snippet: AuNPs were monitored by scanning the surface plasmon peak using ultraviolet-visible spectroscopy (UV-Vis) (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: SDS Page, Western Blot, Staining, Molecular Weight, Transformation Assay, Plasmid Preparation, Purification, Transmission Assay, Polyacrylamide Gel Electrophoresis, Spectroscopy