Staining:Article Title: Effects of long-term salicylate administration on synaptic ultrastructure and metabolic activity in the rat CNS
Article Snippet: .. A consecutive series of ultrathin (70 μm) sections in the coronal plane were prepared using a diamond knife and then stained with lead citrate and observed under a CM-120 TEM (Philips Innovation Services, Eindhoven, the Netherlands). .. ImageJ software was used for quantitative analysis of six sections of each tissue block for each of the two hemispheres.
Article Title: Regeneration of defective epithelial basement membrane and restoration of corneal transparency
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..
Article Title: Optic Neuropathy Due to Microbead-Induced Elevated Intraocular Pressure in the Mouse
Article Snippet: .. Ultrathin (60–90 nm) optic nerve cross-sections were then prepared and stained with uranyl acetate and lead citrate and were examined with a transmission electron microscope (EM410; Philips, Eindhoven, The Netherlands). ..
Article Title: Transmission Electron Microscopy Analysis of Epithelial Basement Membrane Repair in Rabbit Corneas With Haze
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..
Article Title: Loss of SynDIG1 Reduces Excitatory Synapse Maturation But Not Formation In Vivo
Article Snippet: .. Ultrathin 70 nm sections were obtained using a Reichert-Jung Ultramicrotome, thin sections were collected on formvar-coated, single-slot copper grids, stained with uranyl acetate and lead citrate, and examined with a Philips CM-120 electron microscope at 80 kV. ..
Transmission Electron Microscopy:Article Title: Effects of long-term salicylate administration on synaptic ultrastructure and metabolic activity in the rat CNS
Article Snippet: .. A consecutive series of ultrathin (70 μm) sections in the coronal plane were prepared using a diamond knife and then stained with lead citrate and observed under a CM-120 TEM (Philips Innovation Services, Eindhoven, the Netherlands). .. ImageJ software was used for quantitative analysis of six sections of each tissue block for each of the two hemispheres.
Microscopy:Article Title: Defective Laminin 5 Processing in Cylindroma Cells
Article Snippet: .. Ultrathin (60 to 80 nm) sections were prepared with an Ultracut (Reichert-Jung), contrasted with uranyl acetate and lead citrate, and observed with a Philips 201 transmission electron microscope operating at 80 kV. .. For indirect immunofluorescence staining primary mouse monoclonal antibodies against laminin chains included BM165 against α3, GB3 against γ2 (Seralab), and 4C7 against native α5 chain (Life Technologies).
Article Title: Regeneration of defective epithelial basement membrane and restoration of corneal transparency
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..
Article Title: Optic Neuropathy Due to Microbead-Induced Elevated Intraocular Pressure in the Mouse
Article Snippet: .. Ultrathin (60–90 nm) optic nerve cross-sections were then prepared and stained with uranyl acetate and lead citrate and were examined with a transmission electron microscope (EM410; Philips, Eindhoven, The Netherlands). ..
Article Title: Transmission Electron Microscopy Analysis of Epithelial Basement Membrane Repair in Rabbit Corneas With Haze
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..
Article Title: Metabotropic Glutamate Receptor 8-Expressing Nerve Terminals Target Subsets of GABAergic Neurons in the Hippocampus
Article Snippet: .. Ultrathin (70- to 80-nm-thick) serial sections were collected on pioloform-coated copper grids and analyzed in a Philips (Eindhoven, The Netherlands) CM100 electron microscope. .. Unless stated otherwise, samples were obtained at least from two different rat brains, and two blocks of each animal were cut for electron microscopy.
Article Title: Loss of SynDIG1 Reduces Excitatory Synapse Maturation But Not Formation In Vivo
Article Snippet: .. Ultrathin 70 nm sections were obtained using a Reichert-Jung Ultramicrotome, thin sections were collected on formvar-coated, single-slot copper grids, stained with uranyl acetate and lead citrate, and examined with a Philips CM-120 electron microscope at 80 kV. ..
Transmission Assay:Article Title: Defective Laminin 5 Processing in Cylindroma Cells
Article Snippet: .. Ultrathin (60 to 80 nm) sections were prepared with an Ultracut (Reichert-Jung), contrasted with uranyl acetate and lead citrate, and observed with a Philips 201 transmission electron microscope operating at 80 kV. .. For indirect immunofluorescence staining primary mouse monoclonal antibodies against laminin chains included BM165 against α3, GB3 against γ2 (Seralab), and 4C7 against native α5 chain (Life Technologies).
Article Title: Regeneration of defective epithelial basement membrane and restoration of corneal transparency
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..
Article Title: Optic Neuropathy Due to Microbead-Induced Elevated Intraocular Pressure in the Mouse
Article Snippet: .. Ultrathin (60–90 nm) optic nerve cross-sections were then prepared and stained with uranyl acetate and lead citrate and were examined with a transmission electron microscope (EM410; Philips, Eindhoven, The Netherlands). ..
Article Title: Transmission Electron Microscopy Analysis of Epithelial Basement Membrane Repair in Rabbit Corneas With Haze
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..
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