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Philips Healthcare ultrathin
Ultrathin, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ultrathin/product/Philips Healthcare
Average 90 stars, based on 6 article reviews
Price from $9.99 to $1999.99
ultrathin - by Bioz Stars, 2020-08
90/100 stars

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Staining:

Article Title: Effects of long-term salicylate administration on synaptic ultrastructure and metabolic activity in the rat CNS
Article Snippet: .. A consecutive series of ultrathin (70 μm) sections in the coronal plane were prepared using a diamond knife and then stained with lead citrate and observed under a CM-120 TEM (Philips Innovation Services, Eindhoven, the Netherlands). .. ImageJ software was used for quantitative analysis of six sections of each tissue block for each of the two hemispheres.

Article Title: Regeneration of defective epithelial basement membrane and restoration of corneal transparency
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..

Article Title: Optic Neuropathy Due to Microbead-Induced Elevated Intraocular Pressure in the Mouse
Article Snippet: .. Ultrathin (60–90 nm) optic nerve cross-sections were then prepared and stained with uranyl acetate and lead citrate and were examined with a transmission electron microscope (EM410; Philips, Eindhoven, The Netherlands). ..

Article Title: Transmission Electron Microscopy Analysis of Epithelial Basement Membrane Repair in Rabbit Corneas With Haze
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..

Article Title: Loss of SynDIG1 Reduces Excitatory Synapse Maturation But Not Formation In Vivo
Article Snippet: .. Ultrathin 70 nm sections were obtained using a Reichert-Jung Ultramicrotome, thin sections were collected on formvar-coated, single-slot copper grids, stained with uranyl acetate and lead citrate, and examined with a Philips CM-120 electron microscope at 80 kV. ..

Transmission Electron Microscopy:

Article Title: Effects of long-term salicylate administration on synaptic ultrastructure and metabolic activity in the rat CNS
Article Snippet: .. A consecutive series of ultrathin (70 μm) sections in the coronal plane were prepared using a diamond knife and then stained with lead citrate and observed under a CM-120 TEM (Philips Innovation Services, Eindhoven, the Netherlands). .. ImageJ software was used for quantitative analysis of six sections of each tissue block for each of the two hemispheres.

Microscopy:

Article Title: Defective Laminin 5 Processing in Cylindroma Cells
Article Snippet: .. Ultrathin (60 to 80 nm) sections were prepared with an Ultracut (Reichert-Jung), contrasted with uranyl acetate and lead citrate, and observed with a Philips 201 transmission electron microscope operating at 80 kV. .. For indirect immunofluorescence staining primary mouse monoclonal antibodies against laminin chains included BM165 against α3, GB3 against γ2 (Seralab), and 4C7 against native α5 chain (Life Technologies).

Article Title: Regeneration of defective epithelial basement membrane and restoration of corneal transparency
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..

Article Title: Optic Neuropathy Due to Microbead-Induced Elevated Intraocular Pressure in the Mouse
Article Snippet: .. Ultrathin (60–90 nm) optic nerve cross-sections were then prepared and stained with uranyl acetate and lead citrate and were examined with a transmission electron microscope (EM410; Philips, Eindhoven, The Netherlands). ..

Article Title: Transmission Electron Microscopy Analysis of Epithelial Basement Membrane Repair in Rabbit Corneas With Haze
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..

Article Title: Metabotropic Glutamate Receptor 8-Expressing Nerve Terminals Target Subsets of GABAergic Neurons in the Hippocampus
Article Snippet: .. Ultrathin (70- to 80-nm-thick) serial sections were collected on pioloform-coated copper grids and analyzed in a Philips (Eindhoven, The Netherlands) CM100 electron microscope. .. Unless stated otherwise, samples were obtained at least from two different rat brains, and two blocks of each animal were cut for electron microscopy.

Article Title: Loss of SynDIG1 Reduces Excitatory Synapse Maturation But Not Formation In Vivo
Article Snippet: .. Ultrathin 70 nm sections were obtained using a Reichert-Jung Ultramicrotome, thin sections were collected on formvar-coated, single-slot copper grids, stained with uranyl acetate and lead citrate, and examined with a Philips CM-120 electron microscope at 80 kV. ..

Transmission Assay:

Article Title: Defective Laminin 5 Processing in Cylindroma Cells
Article Snippet: .. Ultrathin (60 to 80 nm) sections were prepared with an Ultracut (Reichert-Jung), contrasted with uranyl acetate and lead citrate, and observed with a Philips 201 transmission electron microscope operating at 80 kV. .. For indirect immunofluorescence staining primary mouse monoclonal antibodies against laminin chains included BM165 against α3, GB3 against γ2 (Seralab), and 4C7 against native α5 chain (Life Technologies).

Article Title: Regeneration of defective epithelial basement membrane and restoration of corneal transparency
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..

Article Title: Optic Neuropathy Due to Microbead-Induced Elevated Intraocular Pressure in the Mouse
Article Snippet: .. Ultrathin (60–90 nm) optic nerve cross-sections were then prepared and stained with uranyl acetate and lead citrate and were examined with a transmission electron microscope (EM410; Philips, Eindhoven, The Netherlands). ..

Article Title: Transmission Electron Microscopy Analysis of Epithelial Basement Membrane Repair in Rabbit Corneas With Haze
Article Snippet: .. Ultrathin 85 nm thick sections were cut with a diamond knife, stained with 5% uranyl acetate and lead citrate, and then observed using a Philips CM12 transmission electron microscope operated at 60 kV (FEI Company, Hillsboro, OR). ..

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    Philips Healthcare ultrathin sections
    Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and <t>ultrathin</t> sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.
    Ultrathin Sections, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1005 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/Philips Healthcare
    Average 94 stars, based on 1005 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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    Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and ultrathin sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.

    Journal: Journal of Virology

    Article Title: Fiberless Recombinant Adenoviruses: Virus Maturation and Infectivity in the Absence of Fiber

    doi:

    Figure Lengend Snippet: Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and ultrathin sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.

    Article Snippet: Ultrathin sections were observed under a Philips CM120 Biotwin electron microscope at 120 kV.

    Techniques: Electron Microscopy, Infection, Produced, Staining

    Abnormal morphology and cellular distribution of mitochondria in the mutant blastocysts. Ultrathin sections of the blastocysts were examined with a transmission electron microscope. The mitochondria of the normal (A and C) and the mutant (B and D) cells are indicated by arrows. Scale bar = 1 μm.

    Journal: Molecular and Cellular Biology

    Article Title: GRIM-19, a Cell Death Regulatory Protein, Is Essential for Assembly and Function of Mitochondrial Complex I

    doi: 10.1128/MCB.24.19.8447-8456.2004

    Figure Lengend Snippet: Abnormal morphology and cellular distribution of mitochondria in the mutant blastocysts. Ultrathin sections of the blastocysts were examined with a transmission electron microscope. The mitochondria of the normal (A and C) and the mutant (B and D) cells are indicated by arrows. Scale bar = 1 μm.

    Article Snippet: Ultrathin sections were stained with uranyl acetate and lead citrate and examined with an EM208 transmission electron microscope (Philips Electron Optics).

    Techniques: Mutagenesis, Transmission Assay, Microscopy

    Electron microscopy of purified virus particles. Particles purified by sucrose gradient centrifugation from HeLa cells infected with WR or vA32i in the absence of IPTG were diluted, collected by high-speed centrifugation, fixed in glutaraldehyde, and embedded in Epon. Ultrathin sections were examined by electron microscopy.

    Journal: Journal of Virology

    Article Title: DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles

    doi:

    Figure Lengend Snippet: Electron microscopy of purified virus particles. Particles purified by sucrose gradient centrifugation from HeLa cells infected with WR or vA32i in the absence of IPTG were diluted, collected by high-speed centrifugation, fixed in glutaraldehyde, and embedded in Epon. Ultrathin sections were examined by electron microscopy.

    Article Snippet: Ultrathin sections of infected cells and virions were viewed with a Philips CM100 electron microscope.

    Techniques: Electron Microscopy, Purification, Gradient Centrifugation, Infection, Centrifugation

    Morphogenesis of mutant viruses. BS-C-1 cells were infected with vA32/A32i (A and C) or vA32i (B and D) in the absence of IPTG. After 24 h, the cells were fixed in glutaraldehyde and embedded in Epon, and then ultrathin sections were prepared for electron microscopy. Cr, crescents; Nu, nucleoids; IMV, intracellular mature virions; IV, immature virions; DIV, dense immature virions.

    Journal: Journal of Virology

    Article Title: DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles

    doi:

    Figure Lengend Snippet: Morphogenesis of mutant viruses. BS-C-1 cells were infected with vA32/A32i (A and C) or vA32i (B and D) in the absence of IPTG. After 24 h, the cells were fixed in glutaraldehyde and embedded in Epon, and then ultrathin sections were prepared for electron microscopy. Cr, crescents; Nu, nucleoids; IMV, intracellular mature virions; IV, immature virions; DIV, dense immature virions.

    Article Snippet: Ultrathin sections of infected cells and virions were viewed with a Philips CM100 electron microscope.

    Techniques: Mutagenesis, Infection, Electron Microscopy

    Transmission electron micrographs of ultrathin sections of knots induced by P. savastanoi pv. savastanoi NCPPB‐3335‐GFP at 35 dpi on in vitro olive plants. A. Ultrastructure of knot tissue showing parenchymatic‐like cells containing a fibrillar cytoplasm and irregular cell wall thickenings (arrowhead). Bacterial cells were visualized at intercellular spaces (black arrow). B. Pseudomonas savastanoi pv. savastanoi cells (black arrow) localized at the intercellular space of two host cells. A condensed cytoplasm (asterisk) and a degraded middle lamella are shown. C. Bacterial cells (black arrow) in contact with a primary cell wall in the process of degradation (arrowhead). D. Abnormal cell wall accumulations of host cells in close contact with degenerated cytoplasm (asterisks) and pathogen cells (black arrow). E. Rod‐shaped (black arrow) and irregular (open arrow) bacterial cells colonizing the extracellular space of a host cell showing degenerated organelles (asterisks). F. Group of P. savastanoi pv. savastanoi cells within a degenerated host cell. Bacterial cells are seen surrounded by an electrolucent halo and immersed in a fibroreticular matrix. G. Detail of (F), high‐magnification image of the bacterial surface releasing outer‐membrane vesicles (arrowheads). H. Detail of the fusion of outer‐membrane vesicles (arrowhead) to the fibroreticular matrix.

    Journal: Microbial biotechnology

    Article Title: Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots

    doi: 10.1111/j.1751-7915.2009.00101.x

    Figure Lengend Snippet: Transmission electron micrographs of ultrathin sections of knots induced by P. savastanoi pv. savastanoi NCPPB‐3335‐GFP at 35 dpi on in vitro olive plants. A. Ultrastructure of knot tissue showing parenchymatic‐like cells containing a fibrillar cytoplasm and irregular cell wall thickenings (arrowhead). Bacterial cells were visualized at intercellular spaces (black arrow). B. Pseudomonas savastanoi pv. savastanoi cells (black arrow) localized at the intercellular space of two host cells. A condensed cytoplasm (asterisk) and a degraded middle lamella are shown. C. Bacterial cells (black arrow) in contact with a primary cell wall in the process of degradation (arrowhead). D. Abnormal cell wall accumulations of host cells in close contact with degenerated cytoplasm (asterisks) and pathogen cells (black arrow). E. Rod‐shaped (black arrow) and irregular (open arrow) bacterial cells colonizing the extracellular space of a host cell showing degenerated organelles (asterisks). F. Group of P. savastanoi pv. savastanoi cells within a degenerated host cell. Bacterial cells are seen surrounded by an electrolucent halo and immersed in a fibroreticular matrix. G. Detail of (F), high‐magnification image of the bacterial surface releasing outer‐membrane vesicles (arrowheads). H. Detail of the fusion of outer‐membrane vesicles (arrowhead) to the fibroreticular matrix.

    Article Snippet: Ultrathin sections were mounted in grids, stained using Reynold's lead citrate solution and uranyl acetate and visualized using a Philips CM100 electron microscope.

    Techniques: Transmission Assay, In Vitro