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Hitachi Ltd ultrathin
Ultrathin, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 16 article reviews
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ultrathin - by Bioz Stars, 2020-08
91/100 stars

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Related Articles

Staining:

Article Title: A Conserved, Mg2+-Dependent Exonuclease Degrades Organelle DNA during Arabidopsis Pollen Development [C] Pollen Development [C] [W]
Article Snippet: .. Ultrathin (70 to 90 nm) sections were stained in 1% (w/v) uranyl acetate and 0.5% (w/v) lead citrate and examined using an electron microscope (H-7100; Hitachi) operating at 75 kV. .. To construct pG002926940 for complementation of dpd1 , a DNA fragment containing the At5g26940 genomic sequence was amplified using PCR with primers: 5′-GTTunderline > GGTACC/underline > TTGTAGCTCTGTTTTGGCCTA-3′ ( Kpn I site underlined) and 5′-GCAunderline > GAGCTC/underline > ATGATGTTCCCTTATAATTAG-3′ ( Sac I site underlined).

Article Title: In Vitro Replication of Chelonid Herpesvirus 5 in Organotypic Skin Cultures from Hawaiian Green Turtles ( Chelonia mydas)
Article Snippet: .. For electron microscopy, ultrathin (60 to 80 nm) sections were obtained on an RMC Powertome ultramicrotome, double stained with uranyl acetate and lead citrate, viewed on a Hitachi HT7700 transmission electron microscope (TEM) at 100 kV, and photographed with an AMT XR-41B 2k-by-2k charge-coupled device (CCD) camera. ..

Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
Article Snippet: .. The specimens were sectioned (70 nm ultrathin) with a Leica EM UC7 ultramicrotome, and sections were stained with uranyl acetate and alkaline lead citrate for 10 min. TEM was performed using a Hitachi model H-7650. .. Histochemical staining and ROS-scavenging enzyme assays DAB and NBT staining were performed on leaves from wild-type and wls5 plants to detect H2 O2 and superoxide anions, as described by Blum and Ebercon ( ).

Article Title: A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence 1 Gene Is Required for Plant Growth and Leaf Senescence 1 [OPEN]
Article Snippet: .. The specimens were then sectioned (70 nm ultrathin) with a Leica EM UC7 ultratome, and the sections were stained by uranyl acetate and alkaline lead citrate for 10 min before being observed by with a Hitachi model H-7650. ..

Electron Microscopy:

Article Title: In Vitro Replication of Chelonid Herpesvirus 5 in Organotypic Skin Cultures from Hawaiian Green Turtles ( Chelonia mydas)
Article Snippet: .. For electron microscopy, ultrathin (60 to 80 nm) sections were obtained on an RMC Powertome ultramicrotome, double stained with uranyl acetate and lead citrate, viewed on a Hitachi HT7700 transmission electron microscope (TEM) at 100 kV, and photographed with an AMT XR-41B 2k-by-2k charge-coupled device (CCD) camera. ..

Article Title: Synergistic effects of HMG‐CoA reductase inhibitor and angiotensin II receptor blocker on load‐induced heart failure
Article Snippet: .. Ultrathin 90‐nm sections were collected on copper grids, double‐stained with uranyl acetate and lead citrate, and then observed using transmission electron microscopy (H‐7011; Hitachi, Tokyo, Japan). ..

Microscopy:

Article Title: A Conserved, Mg2+-Dependent Exonuclease Degrades Organelle DNA during Arabidopsis Pollen Development [C] Pollen Development [C] [W]
Article Snippet: .. Ultrathin (70 to 90 nm) sections were stained in 1% (w/v) uranyl acetate and 0.5% (w/v) lead citrate and examined using an electron microscope (H-7100; Hitachi) operating at 75 kV. .. To construct pG002926940 for complementation of dpd1 , a DNA fragment containing the At5g26940 genomic sequence was amplified using PCR with primers: 5′-GTTunderline > GGTACC/underline > TTGTAGCTCTGTTTTGGCCTA-3′ ( Kpn I site underlined) and 5′-GCAunderline > GAGCTC/underline > ATGATGTTCCCTTATAATTAG-3′ ( Sac I site underlined).

Article Title: In Vitro Replication of Chelonid Herpesvirus 5 in Organotypic Skin Cultures from Hawaiian Green Turtles ( Chelonia mydas)
Article Snippet: .. For electron microscopy, ultrathin (60 to 80 nm) sections were obtained on an RMC Powertome ultramicrotome, double stained with uranyl acetate and lead citrate, viewed on a Hitachi HT7700 transmission electron microscope (TEM) at 100 kV, and photographed with an AMT XR-41B 2k-by-2k charge-coupled device (CCD) camera. ..

Article Title: UNC-13 is required for synaptic vesicle fusion in C. elegans
Article Snippet: .. Ribbons of ultrathin (~35 nm) sections were collected and examined on a Hitachi H-7100 electron microscope equipped with a Gatan slow-scan digital camera. .. Morphometric analysis was done with the public-domain software package NIH Image.

Article Title: Endometrial Receptivity Defects and Impaired Implantation in Diabetic NOD Mice
Article Snippet: .. Ultrathin (1-μm-thick) sections were prepared from selected regions of epoxy-embedded implantation sites and counterstained for 10 min with 4% aqueous uranyl acetate, followed by 2 min of treatment with lead citrate, and viewed with a Hitachi model 7000 transmission electron microscope operated at 75 kV .. Uterine samples collected from mated normoglycemic and dNOD mice were homogenized in homogenization buffer (cytosolic and nuclear extraction buffer kit; Biovision Inc.) using an ice-cold, sterile glass Dounce-tissue homogenizer.

Article Title: Disassembly of the fruit cell wall by the ripening-associated polygalacturonase and expansin influences tomato cracking
Article Snippet: .. Ultrathin fixed and embedded fruit tissue sections were examined with a Hitachi-7650 transmission electron microscope. ..

Transmission Electron Microscopy:

Article Title: In Vitro Replication of Chelonid Herpesvirus 5 in Organotypic Skin Cultures from Hawaiian Green Turtles ( Chelonia mydas)
Article Snippet: .. For electron microscopy, ultrathin (60 to 80 nm) sections were obtained on an RMC Powertome ultramicrotome, double stained with uranyl acetate and lead citrate, viewed on a Hitachi HT7700 transmission electron microscope (TEM) at 100 kV, and photographed with an AMT XR-41B 2k-by-2k charge-coupled device (CCD) camera. ..

Article Title: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
Article Snippet: .. The specimens were sectioned (70 nm ultrathin) with a Leica EM UC7 ultramicrotome, and sections were stained with uranyl acetate and alkaline lead citrate for 10 min. TEM was performed using a Hitachi model H-7650. .. Histochemical staining and ROS-scavenging enzyme assays DAB and NBT staining were performed on leaves from wild-type and wls5 plants to detect H2 O2 and superoxide anions, as described by Blum and Ebercon ( ).

Transmission Assay:

Article Title: In Vitro Replication of Chelonid Herpesvirus 5 in Organotypic Skin Cultures from Hawaiian Green Turtles ( Chelonia mydas)
Article Snippet: .. For electron microscopy, ultrathin (60 to 80 nm) sections were obtained on an RMC Powertome ultramicrotome, double stained with uranyl acetate and lead citrate, viewed on a Hitachi HT7700 transmission electron microscope (TEM) at 100 kV, and photographed with an AMT XR-41B 2k-by-2k charge-coupled device (CCD) camera. ..

Article Title: Synergistic effects of HMG‐CoA reductase inhibitor and angiotensin II receptor blocker on load‐induced heart failure
Article Snippet: .. Ultrathin 90‐nm sections were collected on copper grids, double‐stained with uranyl acetate and lead citrate, and then observed using transmission electron microscopy (H‐7011; Hitachi, Tokyo, Japan). ..

Article Title: Endometrial Receptivity Defects and Impaired Implantation in Diabetic NOD Mice
Article Snippet: .. Ultrathin (1-μm-thick) sections were prepared from selected regions of epoxy-embedded implantation sites and counterstained for 10 min with 4% aqueous uranyl acetate, followed by 2 min of treatment with lead citrate, and viewed with a Hitachi model 7000 transmission electron microscope operated at 75 kV .. Uterine samples collected from mated normoglycemic and dNOD mice were homogenized in homogenization buffer (cytosolic and nuclear extraction buffer kit; Biovision Inc.) using an ice-cold, sterile glass Dounce-tissue homogenizer.

Article Title: Disassembly of the fruit cell wall by the ripening-associated polygalacturonase and expansin influences tomato cracking
Article Snippet: .. Ultrathin fixed and embedded fruit tissue sections were examined with a Hitachi-7650 transmission electron microscope. ..

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    Hitachi Ltd ultrafine structure
    Morphology and electronic microscopy of islets. Morphology and the <t>ultrafine</t> structure was evaluated using a phase-contrast microscope and a scanning electron microscope (SEM). The Y-27632 group maintained form better than the control group with respect
    Ultrafine Structure, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrafine structure/product/Hitachi Ltd
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultrafine structure - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    91
    Hitachi Ltd ultrathin section
    Changes in the ultrastructure of the bile canaliculi in porcine hepatocytes after warm ischemia. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated porcine liver graft samples after warm ischemia for 60 minutes. The partial area indicated in A was further photographed at a higher magnification (B). Bile canaliculi are colored green, nuclei are colored blue, huge vacuoles are colored red. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. (C) Typical bile canaliculi were identified in the <t>ultrathin</t> sections of Epon 812-embedded tissues from liver graft samples after warm ischemia for 60 minutes. Bile canaliculi are colored green and asterisks indicate the vacant space without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.
    Ultrathin Section, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin section/product/Hitachi Ltd
    Average 91 stars, based on 1235 article reviews
    Price from $9.99 to $1999.99
    ultrathin section - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Morphology and electronic microscopy of islets. Morphology and the ultrafine structure was evaluated using a phase-contrast microscope and a scanning electron microscope (SEM). The Y-27632 group maintained form better than the control group with respect

    Journal: Cell Medicine

    Article Title: Maintenance of Viability and Function of Rat Islets With the Use of ROCK Inhibitor Y-27632

    doi: 10.3727/215517913X674199

    Figure Lengend Snippet: Morphology and electronic microscopy of islets. Morphology and the ultrafine structure was evaluated using a phase-contrast microscope and a scanning electron microscope (SEM). The Y-27632 group maintained form better than the control group with respect

    Article Snippet: In addition, the ultrafine structure was evaluated in the same manner using a scanning electron microscope (SEM) (Hitachi, Tokyo, Japan).

    Techniques: Microscopy

    Changes in the ultrastructure of the bile canaliculi in porcine hepatocytes after warm ischemia. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated porcine liver graft samples after warm ischemia for 60 minutes. The partial area indicated in A was further photographed at a higher magnification (B). Bile canaliculi are colored green, nuclei are colored blue, huge vacuoles are colored red. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. (C) Typical bile canaliculi were identified in the ultrathin sections of Epon 812-embedded tissues from liver graft samples after warm ischemia for 60 minutes. Bile canaliculi are colored green and asterisks indicate the vacant space without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.

    Journal: PLoS ONE

    Article Title: The ultrastructural characteristics of bile canaliculus in porcine liver donated after cardiac death and machine perfusion preservation

    doi: 10.1371/journal.pone.0233917

    Figure Lengend Snippet: Changes in the ultrastructure of the bile canaliculi in porcine hepatocytes after warm ischemia. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated porcine liver graft samples after warm ischemia for 60 minutes. The partial area indicated in A was further photographed at a higher magnification (B). Bile canaliculi are colored green, nuclei are colored blue, huge vacuoles are colored red. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. (C) Typical bile canaliculi were identified in the ultrathin sections of Epon 812-embedded tissues from liver graft samples after warm ischemia for 60 minutes. Bile canaliculi are colored green and asterisks indicate the vacant space without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.

    Article Snippet: Ultrathin section (80 nm thick) were cut, stained with uranyl acetate and lead citrate, and observed using an HT7700 transmission electron microscope (Hitachi High Technologies, Tokyo, Japan).

    Techniques:

    The ultrastructure of the bile canaliculi in porcine hepatocytes of the control liver. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated control porcine liver graft samples. The partial area indicated in A was further photographed at a higher magnification (B). (C) Typical bile canaliculi were identified in the ultrathin sections of the Epon 812-embedded control liver tissue. Bile canaliculi are colored green. Arrows indicate the Golgi apparatus, and asterisks indicate vacant spaces without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.

    Journal: PLoS ONE

    Article Title: The ultrastructural characteristics of bile canaliculus in porcine liver donated after cardiac death and machine perfusion preservation

    doi: 10.1371/journal.pone.0233917

    Figure Lengend Snippet: The ultrastructure of the bile canaliculi in porcine hepatocytes of the control liver. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated control porcine liver graft samples. The partial area indicated in A was further photographed at a higher magnification (B). (C) Typical bile canaliculi were identified in the ultrathin sections of the Epon 812-embedded control liver tissue. Bile canaliculi are colored green. Arrows indicate the Golgi apparatus, and asterisks indicate vacant spaces without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.

    Article Snippet: Ultrathin section (80 nm thick) were cut, stained with uranyl acetate and lead citrate, and observed using an HT7700 transmission electron microscope (Hitachi High Technologies, Tokyo, Japan).

    Techniques:

    The ultrastructural changes of the bile canaliculi in porcine liver grafts preserved by HMP. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated porcine liver graft samples preserved by HMP for 4 h after 60 minutes of warm ischemia. The partial area indicated in A was further photographed under higher magnification (B). Bile canaliculi are colored green, nuclei are colored blue. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. (C) Typical bile canaliculi were identified in the ultrathin sections of Epon 812-embedded tissues from liver graft samples preserved by HMP for 4 h after 60 minutes of warm ischemia. Bile canaliculi are colored green. Asterisks indicate the vacant space without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.

    Journal: PLoS ONE

    Article Title: The ultrastructural characteristics of bile canaliculus in porcine liver donated after cardiac death and machine perfusion preservation

    doi: 10.1371/journal.pone.0233917

    Figure Lengend Snippet: The ultrastructural changes of the bile canaliculi in porcine liver grafts preserved by HMP. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated porcine liver graft samples preserved by HMP for 4 h after 60 minutes of warm ischemia. The partial area indicated in A was further photographed under higher magnification (B). Bile canaliculi are colored green, nuclei are colored blue. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. (C) Typical bile canaliculi were identified in the ultrathin sections of Epon 812-embedded tissues from liver graft samples preserved by HMP for 4 h after 60 minutes of warm ischemia. Bile canaliculi are colored green. Asterisks indicate the vacant space without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.

    Article Snippet: Ultrathin section (80 nm thick) were cut, stained with uranyl acetate and lead citrate, and observed using an HT7700 transmission electron microscope (Hitachi High Technologies, Tokyo, Japan).

    Techniques:

    The ultrastructural changes of the bile canaliculi in porcine liver grafts preserved by MMP. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated porcine liver graft samples preserved by MMP for 4 h after 60 minutes of warm ischemia. The partial area indicated in A was further photographed under higher magnification (B). Bile canaliculi are colored green, nuclei are colored blue, huge vacuoles are colored red. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. (C) Typical bile canaliculi were identified in the ultrathin sections of the Epon 812-embedded tissues from liver graft samples preserved by MMP for 4 h after 60 minutes of warm ischemia. Bile canaliculi are colored green. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.

    Journal: PLoS ONE

    Article Title: The ultrastructural characteristics of bile canaliculus in porcine liver donated after cardiac death and machine perfusion preservation

    doi: 10.1371/journal.pone.0233917

    Figure Lengend Snippet: The ultrastructural changes of the bile canaliculi in porcine liver grafts preserved by MMP. (A and B) Representative hepatocytes and bile canaliculi were observed by SEM in osmium-macerated porcine liver graft samples preserved by MMP for 4 h after 60 minutes of warm ischemia. The partial area indicated in A was further photographed under higher magnification (B). Bile canaliculi are colored green, nuclei are colored blue, huge vacuoles are colored red. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. (C) Typical bile canaliculi were identified in the ultrathin sections of the Epon 812-embedded tissues from liver graft samples preserved by MMP for 4 h after 60 minutes of warm ischemia. Bile canaliculi are colored green. Asterisks indicate vacant space without any other endomembrane organelles around the bile canaliculi. Bars = 1 μm.

    Article Snippet: Ultrathin section (80 nm thick) were cut, stained with uranyl acetate and lead citrate, and observed using an HT7700 transmission electron microscope (Hitachi High Technologies, Tokyo, Japan).

    Techniques:

    HS1 is involved in cytosol-to-chloroplast transport of HPT. Subcellular localization of HPT-GFP in the WT (A) and hs1-1 (B) backgrounds in Arabidopsis. HPT-GFP constructs were transformed transiently into plant cells. Arrowheads indicate higher chloroplast GFP fluorescence intensity in a mesophyll protoplast of WT compared to hs1-1 . Bars = 10 μm. (C , D) Western blot of HPT in WT/ HPT (empty vector transgenic lines), HS1 RNAi/ HPT , and hs1-1 / HPT (empty vector transformed into hs1-1 ) transgenic plants. HPT accumulation was reduced significantly or undetectable in HS1 RNAi/ HPT and hs1-1/HPT chloroplasts compared with the control (C) , while total HPT varied slightly (D) . The large subunit of RUBISCO (RBCL) was probed as a protein loading control. Approximately 20 μg (C) and 10 μg (D) of protein were loaded in each lane. Similar results were obtained in two additional independent experiments. (E–H) Transmission electron microscopic images of immunogold localization of HPT in transgenic Arabidopsis . HPT in ultrathin leaf sections reacted with anti-HPT antibody and a gold-conjugated secondary antibody. Gold particles were detected by transmission electron microscopy. Immunogold labeling of HPT in leaves from WT/ HPT (E) , hs1-1 / HPT (F) , hs1-1 / HPT / HS1 (G) , negative control (H) transgenic Arabidopsis plants, and negative control without anti-HPT antibody in blocking buffer showed no gold particles. Arrowheads show typical gold particles (10 nm, black dots). HPT present inside of chloroplasts (chl, the dark area) in WT / HPT , hs1-1 / HPT / HS1 , and outside of chloroplasts in hs1-1 / HPT . Three different leaf samples and more than 30 immunogold-labeled positive cells were observed with similar results. Images represent typical observations in different leaf samples. chl, chloroplast; cyt, cytosol; va, vacuole; cw, cell wall. Bars = 1 μm.

    Journal: Frontiers in Plant Science

    Article Title: HS1 Is Involved in Hygromycin Resistance Through Facilitating Hygromycin Phosphotransferase Transportation From Cytosol to Chloroplast

    doi: 10.3389/fpls.2020.00613

    Figure Lengend Snippet: HS1 is involved in cytosol-to-chloroplast transport of HPT. Subcellular localization of HPT-GFP in the WT (A) and hs1-1 (B) backgrounds in Arabidopsis. HPT-GFP constructs were transformed transiently into plant cells. Arrowheads indicate higher chloroplast GFP fluorescence intensity in a mesophyll protoplast of WT compared to hs1-1 . Bars = 10 μm. (C , D) Western blot of HPT in WT/ HPT (empty vector transgenic lines), HS1 RNAi/ HPT , and hs1-1 / HPT (empty vector transformed into hs1-1 ) transgenic plants. HPT accumulation was reduced significantly or undetectable in HS1 RNAi/ HPT and hs1-1/HPT chloroplasts compared with the control (C) , while total HPT varied slightly (D) . The large subunit of RUBISCO (RBCL) was probed as a protein loading control. Approximately 20 μg (C) and 10 μg (D) of protein were loaded in each lane. Similar results were obtained in two additional independent experiments. (E–H) Transmission electron microscopic images of immunogold localization of HPT in transgenic Arabidopsis . HPT in ultrathin leaf sections reacted with anti-HPT antibody and a gold-conjugated secondary antibody. Gold particles were detected by transmission electron microscopy. Immunogold labeling of HPT in leaves from WT/ HPT (E) , hs1-1 / HPT (F) , hs1-1 / HPT / HS1 (G) , negative control (H) transgenic Arabidopsis plants, and negative control without anti-HPT antibody in blocking buffer showed no gold particles. Arrowheads show typical gold particles (10 nm, black dots). HPT present inside of chloroplasts (chl, the dark area) in WT / HPT , hs1-1 / HPT / HS1 , and outside of chloroplasts in hs1-1 / HPT . Three different leaf samples and more than 30 immunogold-labeled positive cells were observed with similar results. Images represent typical observations in different leaf samples. chl, chloroplast; cyt, cytosol; va, vacuole; cw, cell wall. Bars = 1 μm.

    Article Snippet: Immunized ultrathin sections were washed, post-stained, and examined with a transmission electron microscope (Hitachi H-7650, Japan).

    Techniques: Construct, Transformation Assay, Fluorescence, Western Blot, Plasmid Preparation, Transgenic Assay, Transmission Assay, Electron Microscopy, Labeling, Negative Control, Blocking Assay

    Immunogold labeling of core proteins. Cells were infected with WR or Ets85 at an MOI of 10 and incubated at 31°C or 39.7°C. At 24 h postinfection, cells were processed for immunoelectron microscopy. Ultrathin sections were probed with

    Journal: Journal of Virology

    Article Title: Vaccinia Virus Mutations in the L4R Gene Encoding a Virion Structural Protein Produce Abnormal Mature Particles Lacking a Nucleocapsid

    doi: 10.1128/JVI.02126-14

    Figure Lengend Snippet: Immunogold labeling of core proteins. Cells were infected with WR or Ets85 at an MOI of 10 and incubated at 31°C or 39.7°C. At 24 h postinfection, cells were processed for immunoelectron microscopy. Ultrathin sections were probed with

    Article Snippet: Ultrathin sections (70 to 80 nm) were poststained with 2% uranyl acetate and lead citrate and examined with an H-7000 transmission electron microscope (TEM; Hitachi High Technologies America, Inc., Schaumburg, IL) operated at 100 kV.

    Techniques: Labeling, Infection, Incubation, Immuno-Electron Microscopy