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Carl Zeiss ultrathin
Ultrathin, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 15 article reviews
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ultrathin - by Bioz Stars, 2020-08
91/100 stars

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Staining:

Article Title: Regeneration of the digestive system in the crinoid Himerometra robustipinna occurs by transdifferentiation of neurosecretory-like cells
Article Snippet: .. Ultrathin 60 nm sections were stained with 1% uranyl acetate in 10% ethanol, then with lead citrate, and analyzed using Libra 120 and Libra 200FE (Carl Zeiss) transmission electron microscopes. .. Treatment with colchicine For the experiments we used a solution of the mitotic inhibitor colchicine in seawater at a concentration of 10-3 –10-7 M. After autotomy and on days 4 and 7 after the removal of the visceral mass, animals were placed into 2-liter aquariums with the colchicine solution and kept there for 2 days.

Article Title: Supplementation with small-extracellular vesicles from ovarian follicular fluid during in vitro production modulates bovine embryo development
Article Snippet: .. Ultrathin 80nm sections were cut, mounted on 300-mesh nickel grids, stained with uranyl acetate and lead citrate, and examined using a MORGAGNI 268D ZEISS transmission electron microscope at 80 kV. .. PKH67 labeled small-extracellular vesicles and cell uptake The EVs obtained by differential ultracentrifugation were collected from the follicular fluid of bovine ovarian follicles between 3–6 mm in size and were labeled with PKH67 (PKH67GL, Sigma-Aldrich, St. Louis, MO), a green fluorescent dye that labels lipid membranes as described in Da Silveira et al. [ ].

Transmission Assay:

Article Title: Regeneration of the digestive system in the crinoid Himerometra robustipinna occurs by transdifferentiation of neurosecretory-like cells
Article Snippet: .. Ultrathin 60 nm sections were stained with 1% uranyl acetate in 10% ethanol, then with lead citrate, and analyzed using Libra 120 and Libra 200FE (Carl Zeiss) transmission electron microscopes. .. Treatment with colchicine For the experiments we used a solution of the mitotic inhibitor colchicine in seawater at a concentration of 10-3 –10-7 M. After autotomy and on days 4 and 7 after the removal of the visceral mass, animals were placed into 2-liter aquariums with the colchicine solution and kept there for 2 days.

Article Title: In Vivo Evidence for Lysosome Depletion and Impaired Autophagic Clearance in Hereditary Spastic Paraplegia Type SPG11
Article Snippet: .. Ultrathin 80 nm sections (Ultratome III, LKB Instruments) were mounted on filmed copper grids (100 mesh), post-stained with lead citrate, and studied in a transmission electron microscope (EM 900, Zeiss) at 80 kV. .. Primary cell culture Mouse embryonic fibroblasts (MEFs) were prepared from E13.5 mouse embryos as described [ ].

Article Title: The Effect of Autologous Platelet Rich Plasma in the Treatment of Achilles Tendon Ruptures: An Experimental Study on Rabbits
Article Snippet: .. After semi-thin sections were taken with an ultratome (Leica Microsystems; Wetzlar, Germany), ultrathin ( < 0.1 µm) sections were mounted on grids and analyzed using a transmission electron microscope (TEM, Carl Zeiss Libra 120 EFTEM; Stuttgart, Germany). ..

Article Title: Supplementation with small-extracellular vesicles from ovarian follicular fluid during in vitro production modulates bovine embryo development
Article Snippet: .. Ultrathin 80nm sections were cut, mounted on 300-mesh nickel grids, stained with uranyl acetate and lead citrate, and examined using a MORGAGNI 268D ZEISS transmission electron microscope at 80 kV. .. PKH67 labeled small-extracellular vesicles and cell uptake The EVs obtained by differential ultracentrifugation were collected from the follicular fluid of bovine ovarian follicles between 3–6 mm in size and were labeled with PKH67 (PKH67GL, Sigma-Aldrich, St. Louis, MO), a green fluorescent dye that labels lipid membranes as described in Da Silveira et al. [ ].

Article Title: Altered surfactant homeostasis and recurrent respiratory failure secondary to TTF-1 nuclear targeting defect
Article Snippet: .. Ultrathin 1 μ sections obtained from Karnowsky-osmium tetroxide post-fixed and epon-embedded samples contrasted with lead citrate and uranyl acetate were analyzed with a Zeiss 902 transmission electron microscope. .. For quantitative lamellar body analysis, mean count per cell and diameter measurement were derived from10 random sections at 3000 × magnification picturing single type II cell cross-section.

Microscopy:

Article Title: In Vivo Evidence for Lysosome Depletion and Impaired Autophagic Clearance in Hereditary Spastic Paraplegia Type SPG11
Article Snippet: .. Ultrathin 80 nm sections (Ultratome III, LKB Instruments) were mounted on filmed copper grids (100 mesh), post-stained with lead citrate, and studied in a transmission electron microscope (EM 900, Zeiss) at 80 kV. .. Primary cell culture Mouse embryonic fibroblasts (MEFs) were prepared from E13.5 mouse embryos as described [ ].

Article Title: Establishment of a novel retinoblastoma (Rb) nude mouse model by intravitreal injection of human Rb Y79 cells – comparison of in vivo analysis versus histological follow up
Article Snippet: .. For electron microscopy, the sections were cut ultrathin (0.07 µm) and analysed with a Zeiss 902 A electron microscope (Zeiss, Jena, Germany). ..

Article Title: The Effect of Autologous Platelet Rich Plasma in the Treatment of Achilles Tendon Ruptures: An Experimental Study on Rabbits
Article Snippet: .. After semi-thin sections were taken with an ultratome (Leica Microsystems; Wetzlar, Germany), ultrathin ( < 0.1 µm) sections were mounted on grids and analyzed using a transmission electron microscope (TEM, Carl Zeiss Libra 120 EFTEM; Stuttgart, Germany). ..

Article Title: Supplementation with small-extracellular vesicles from ovarian follicular fluid during in vitro production modulates bovine embryo development
Article Snippet: .. Ultrathin 80nm sections were cut, mounted on 300-mesh nickel grids, stained with uranyl acetate and lead citrate, and examined using a MORGAGNI 268D ZEISS transmission electron microscope at 80 kV. .. PKH67 labeled small-extracellular vesicles and cell uptake The EVs obtained by differential ultracentrifugation were collected from the follicular fluid of bovine ovarian follicles between 3–6 mm in size and were labeled with PKH67 (PKH67GL, Sigma-Aldrich, St. Louis, MO), a green fluorescent dye that labels lipid membranes as described in Da Silveira et al. [ ].

Article Title: Altered surfactant homeostasis and recurrent respiratory failure secondary to TTF-1 nuclear targeting defect
Article Snippet: .. Ultrathin 1 μ sections obtained from Karnowsky-osmium tetroxide post-fixed and epon-embedded samples contrasted with lead citrate and uranyl acetate were analyzed with a Zeiss 902 transmission electron microscope. .. For quantitative lamellar body analysis, mean count per cell and diameter measurement were derived from10 random sections at 3000 × magnification picturing single type II cell cross-section.

Transmission Electron Microscopy:

Article Title: The Effect of Autologous Platelet Rich Plasma in the Treatment of Achilles Tendon Ruptures: An Experimental Study on Rabbits
Article Snippet: .. After semi-thin sections were taken with an ultratome (Leica Microsystems; Wetzlar, Germany), ultrathin ( < 0.1 µm) sections were mounted on grids and analyzed using a transmission electron microscope (TEM, Carl Zeiss Libra 120 EFTEM; Stuttgart, Germany). ..

Electron Microscopy:

Article Title: Establishment of a novel retinoblastoma (Rb) nude mouse model by intravitreal injection of human Rb Y79 cells – comparison of in vivo analysis versus histological follow up
Article Snippet: .. For electron microscopy, the sections were cut ultrathin (0.07 µm) and analysed with a Zeiss 902 A electron microscope (Zeiss, Jena, Germany). ..

Article Title: Targeting myelin lipid metabolism as a potential therapeutic strategy in a model of CMT1A neuropathy
Article Snippet: .. For electron microscopy: Ultrathin (50–70 nm) sciatic nerve cross sections were treated with 1% uranylacetate solution and lead citrate and analyzed using a Zeiss EM10 or EM109 (Leo). ..

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    Carl Zeiss ultrathin sections
    Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) <t>Ultrathin</t> section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
    Ultrathin Sections, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 815 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/Carl Zeiss
    Average 94 stars, based on 815 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    94/100 stars
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    Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) Ultrathin section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) Ultrathin section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the lung. (a) Lung of a non-dengue case stained with HE and presenting normal aspect of alveoli (A) and alveolar septa (AS). (b–g) Lung sections of dengue cases, stained with HE, showing pulmonary alterations, including septal thickening (St), edema (E), hemorrhage (He), presence of mononuclear infiltrate (Inf), hyaline membrane formation (HM) and hypertrophy of alveolar macrophages (AM) and type II pneumocytes (PcyII). (h) Semi-thin section of a non-dengue case showing alveoli (A), alveolar septa (AS), endothelial cells (EC) and type I (PcyI) and II pneumocytes (PcyII) with normal aspects. (i) Semi-thin section of one dengue case presenting numerous platelets (Pt) and megakaryocytes (MK) inside alveolar septa. (j) Ultrathin section of one non-dengue case exhibiting regular alveoli, alveolar septum, type I and II pneumocytes and endothelial cell. (j-l) Ultrathin sections of dengue cases exhibited type II pneumocytes located in alveolar space in contact with numerous platelets, the appearance of hyaline membrane and the presence of virus particles (VP) in endothelium. Quantitative analysis of hemorrhage (m) and edema (n) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the lung. (a) Lung of a non-dengue case stained with HE and presenting normal aspect of alveoli (A) and alveolar septa (AS). (b–g) Lung sections of dengue cases, stained with HE, showing pulmonary alterations, including septal thickening (St), edema (E), hemorrhage (He), presence of mononuclear infiltrate (Inf), hyaline membrane formation (HM) and hypertrophy of alveolar macrophages (AM) and type II pneumocytes (PcyII). (h) Semi-thin section of a non-dengue case showing alveoli (A), alveolar septa (AS), endothelial cells (EC) and type I (PcyI) and II pneumocytes (PcyII) with normal aspects. (i) Semi-thin section of one dengue case presenting numerous platelets (Pt) and megakaryocytes (MK) inside alveolar septa. (j) Ultrathin section of one non-dengue case exhibiting regular alveoli, alveolar septum, type I and II pneumocytes and endothelial cell. (j-l) Ultrathin sections of dengue cases exhibited type II pneumocytes located in alveolar space in contact with numerous platelets, the appearance of hyaline membrane and the presence of virus particles (VP) in endothelium. Quantitative analysis of hemorrhage (m) and edema (n) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the spleen. (a) Spleen of a non-dengue case stained with HE and presenting normal aspect. (b and c) Spleen sections of dengue cases, stained with HE, showing vascular congestion (VC), edema (E) and an atrophy of lymphoid follicles. Red pulp (RP); white pulp (WP). (d) Semi-thin section of a non-dengue case revealing red pulp with regular aspect and normal splenocytes (S). (e) Semi-thin section of a dengue case showing vacuolization (V) and degenerated splenocytes (DS). (f) Ultra-thin section of a non-dengue case with regular splenocytes (S) and (g and h) dengue cases exhibiting vacuolization (V) around degenerated splenocytes and loss of the endothelium of splenic sinusoid (SS). Semi-thin and ultrathin sections were stained as described in figure 1 . (i–k) Quantitative analysis of histological damages observed individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). The media of lymphoid follicle areas were quantified (i), as well as the percentage areas with vascular congestion (j) and edema (k). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the spleen. (a) Spleen of a non-dengue case stained with HE and presenting normal aspect. (b and c) Spleen sections of dengue cases, stained with HE, showing vascular congestion (VC), edema (E) and an atrophy of lymphoid follicles. Red pulp (RP); white pulp (WP). (d) Semi-thin section of a non-dengue case revealing red pulp with regular aspect and normal splenocytes (S). (e) Semi-thin section of a dengue case showing vacuolization (V) and degenerated splenocytes (DS). (f) Ultra-thin section of a non-dengue case with regular splenocytes (S) and (g and h) dengue cases exhibiting vacuolization (V) around degenerated splenocytes and loss of the endothelium of splenic sinusoid (SS). Semi-thin and ultrathin sections were stained as described in figure 1 . (i–k) Quantitative analysis of histological damages observed individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). The media of lymphoid follicle areas were quantified (i), as well as the percentage areas with vascular congestion (j) and edema (k). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the heart. (a) Heart of a non-dengue case stained with H.E. and presenting normal aspect. (b and c) Heart sections of dengue cases, stained with HE, showing cardiac injuries, including hemorrhage (He), edema (E), presence of mononuclear infiltrate (Inf) and degeneration of muscle fibers (black star). (d and f) Semi-thin and ultrathin sections of a non-dengue case presenting cardiac fibers (CF) with normal nucleus (N), mitochondria (M), capillaries (Cap) and intercalated discs (ID). (e) Semi-thin section of one dengue case presenting degeneration of cardiac fibers (black star) characterized by absence of nucleus and a diffuse interstitial edema (E) and (g and h) ultrathin sections showing nuclear (white stars) and mitochondria alterations (M) in cardiomyocytes and interstitial edema. Quantitative analysis of hemorrhage (i) and edema (j) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the heart. (a) Heart of a non-dengue case stained with H.E. and presenting normal aspect. (b and c) Heart sections of dengue cases, stained with HE, showing cardiac injuries, including hemorrhage (He), edema (E), presence of mononuclear infiltrate (Inf) and degeneration of muscle fibers (black star). (d and f) Semi-thin and ultrathin sections of a non-dengue case presenting cardiac fibers (CF) with normal nucleus (N), mitochondria (M), capillaries (Cap) and intercalated discs (ID). (e) Semi-thin section of one dengue case presenting degeneration of cardiac fibers (black star) characterized by absence of nucleus and a diffuse interstitial edema (E) and (g and h) ultrathin sections showing nuclear (white stars) and mitochondria alterations (M) in cardiomyocytes and interstitial edema. Quantitative analysis of hemorrhage (i) and edema (j) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological/ultrastructural analysis and dengue detection in the kidney. (a) Kidney of a non-dengue case stained with HE and presenting normal aspect. (b and c) Kidney sections of dengue cases, stained with HE, showing injuries, including: hemorrhage (He), edema (E), sloughing of necrotic cells with loss of the basement membrane (black star), mainly in proximal convoluted tubule (PCT) but also detected in distal convoluted tubule (DCT), and areas of cellular regeneration (blue star) near renal glomerulus (RG). (d and e) Semi-thin sections of non-dengue cases showing Bowman’s capsule (BC) and podocytes (Pdc) around glomerular capillaries (GC), mensagial cells (MC) and endothelial cell (EC) with regular structures and preserved capillaries (Cap), epithelial cells (Ep) and distal and proximal convolutes tubules (DCT and PCT, respectively). (f and g) Dengue cases with the presence of thrombus (T) in capillaries of renal glomerulus and necrotic cells (NC) in the lumen of proximal convoluted tubules. (h) Ultrathin of a non-dengue case exhibiting conserved glomerular capillaries. (i) Ultrathin of one dengue case showing necrotic cell with picnotic nucleus (white star) and dilatation of rough endoplasmic reticulum (ER). Quantitative analysis of hemorrhage (j) and edema (k) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological/ultrastructural analysis and dengue detection in the kidney. (a) Kidney of a non-dengue case stained with HE and presenting normal aspect. (b and c) Kidney sections of dengue cases, stained with HE, showing injuries, including: hemorrhage (He), edema (E), sloughing of necrotic cells with loss of the basement membrane (black star), mainly in proximal convoluted tubule (PCT) but also detected in distal convoluted tubule (DCT), and areas of cellular regeneration (blue star) near renal glomerulus (RG). (d and e) Semi-thin sections of non-dengue cases showing Bowman’s capsule (BC) and podocytes (Pdc) around glomerular capillaries (GC), mensagial cells (MC) and endothelial cell (EC) with regular structures and preserved capillaries (Cap), epithelial cells (Ep) and distal and proximal convolutes tubules (DCT and PCT, respectively). (f and g) Dengue cases with the presence of thrombus (T) in capillaries of renal glomerulus and necrotic cells (NC) in the lumen of proximal convoluted tubules. (h) Ultrathin of a non-dengue case exhibiting conserved glomerular capillaries. (i) Ultrathin of one dengue case showing necrotic cell with picnotic nucleus (white star) and dilatation of rough endoplasmic reticulum (ER). Quantitative analysis of hemorrhage (j) and edema (k) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Electron microscopic confirmation of single nuclei in LacZ-labeled Purkinje neurons in vav-iCre/LacZ mice. a , Example of a LacZ-positive Purkinje neuron, detected by X-gal staining of a 50-μm-thick Vibratome section, located in the Purkinje neuron layer (PC), between molecular (mol) and granule (gr) cell layer. b , Larger image of the same cell as in a . c , Light microscopic staining of calbindin confirming that all large X-gal-positive cell bodies in the PC are indeed Purkinje neurons. Typical dotted X-gal stain in the cell body and dendrite indicated by black arrowheads. d , e , Serial ultrathin sections through the same PKN as shown in a and b reveal only a single nucleus. The nucleus is large and light with a dark basophilic nucleolus, with deep folds and invaginations toward the side of the dendrites and Nissl bodies (NB) at the side of the folds, thus displaying the typical morphologic features of Purkinje neurons. f , g , Same cell at different z -levels and smaller magnification showing no second nucleus in this neuron. Scale bars: a–c , 20 μm; d , e , 2 μm; f , g , 5 μm.

    Journal: The Journal of Neuroscience

    Article Title: Fusion of Hematopoietic Cells with Purkinje Neurons Does Not Lead to Stable Heterokaryon Formation under Noninvasive Conditions

    doi: 10.1523/JNEUROSCI.5848-08.2009

    Figure Lengend Snippet: Electron microscopic confirmation of single nuclei in LacZ-labeled Purkinje neurons in vav-iCre/LacZ mice. a , Example of a LacZ-positive Purkinje neuron, detected by X-gal staining of a 50-μm-thick Vibratome section, located in the Purkinje neuron layer (PC), between molecular (mol) and granule (gr) cell layer. b , Larger image of the same cell as in a . c , Light microscopic staining of calbindin confirming that all large X-gal-positive cell bodies in the PC are indeed Purkinje neurons. Typical dotted X-gal stain in the cell body and dendrite indicated by black arrowheads. d , e , Serial ultrathin sections through the same PKN as shown in a and b reveal only a single nucleus. The nucleus is large and light with a dark basophilic nucleolus, with deep folds and invaginations toward the side of the dendrites and Nissl bodies (NB) at the side of the folds, thus displaying the typical morphologic features of Purkinje neurons. f , g , Same cell at different z -levels and smaller magnification showing no second nucleus in this neuron. Scale bars: a–c , 20 μm; d , e , 2 μm; f , g , 5 μm.

    Article Snippet: Ultrathin sections (50 nm; ∼200 per LacZ-expressing cell) were serially cut on a Leica Ultracut UCT, stained with uranyl acetate and lead citrate, and analyzed using a Zeiss EM 10CR TEM.

    Techniques: Labeling, Mouse Assay, Staining

    Vesicles and synapses of neuronal profiles in the CBL. Details from frontal ultrathin sections through the CBL. (A) Central neuropil area showing neuronal profiles with different vesicle content (N1–N3) and processes from a glial cell (G). N1, neuronal profile with large granular vesicles (arrowheads); N2, neuronal profile with small clear pleomorphic vesicles and large dense core vesicles; N3, profile with circular small clear vesicles (arrows) and large ovoid dense core vesicles (white arrowheads). Note the different appearance of the small vesicles in N2 and N3. (B) Central neuropil area. Neuronal profile with large granular vesicles (N1) and two dyadic output synapses (s1, s2, arrowheads). Both synapses are of type I, i.e., one presynaptic profile (N1) faces two postsynaptic profiles (N2 and N3, resp. N3 and N4). Inset shows synaptic profiles at higher magnification (45,000×). Both synapses show a presynaptic membrane density (arrowheads) surrounded by synaptic vesicles. The intercellular space in enlarged at the active zone. The postsynaptic membrane only shows minor accumulation of electron dense material (white arrow). (C) Type II synapse. Two presynaptic profiles (N1,N2) with small clear synaptic vesicles and large granular vesicles face a postsynaptic profile with small clear and large dense core vesicles. Arrowheads point to presynaptic densities with accumulations of clear synaptic vesicles. The asterisk marks a profile with large granular vesicles. The membrane of most vesicles is broken (arrows). These have a lighter granular content than intact vesicles (white arrowheads). (D) Magnified detail from Figure 2C . Profiles N1 and N2 with small clear and dark dense core vesicles are presynaptic to a third small profile (asterisk). In adjacent sections, this profile contained large granular vesicles. Presynaptic structures consist of a bar-shaped density surrounded by small clear vesicles. The synapses may be monadic or dyadic. Scale bar in (D) : 0.5 μm (applies to A–D ).

    Journal: Frontiers in Behavioral Neuroscience

    Article Title: Ultrastructure of GABA- and Tachykinin-Immunoreactive Neurons in the Lower Division of the Central Body of the Desert Locust

    doi: 10.3389/fnbeh.2016.00230

    Figure Lengend Snippet: Vesicles and synapses of neuronal profiles in the CBL. Details from frontal ultrathin sections through the CBL. (A) Central neuropil area showing neuronal profiles with different vesicle content (N1–N3) and processes from a glial cell (G). N1, neuronal profile with large granular vesicles (arrowheads); N2, neuronal profile with small clear pleomorphic vesicles and large dense core vesicles; N3, profile with circular small clear vesicles (arrows) and large ovoid dense core vesicles (white arrowheads). Note the different appearance of the small vesicles in N2 and N3. (B) Central neuropil area. Neuronal profile with large granular vesicles (N1) and two dyadic output synapses (s1, s2, arrowheads). Both synapses are of type I, i.e., one presynaptic profile (N1) faces two postsynaptic profiles (N2 and N3, resp. N3 and N4). Inset shows synaptic profiles at higher magnification (45,000×). Both synapses show a presynaptic membrane density (arrowheads) surrounded by synaptic vesicles. The intercellular space in enlarged at the active zone. The postsynaptic membrane only shows minor accumulation of electron dense material (white arrow). (C) Type II synapse. Two presynaptic profiles (N1,N2) with small clear synaptic vesicles and large granular vesicles face a postsynaptic profile with small clear and large dense core vesicles. Arrowheads point to presynaptic densities with accumulations of clear synaptic vesicles. The asterisk marks a profile with large granular vesicles. The membrane of most vesicles is broken (arrows). These have a lighter granular content than intact vesicles (white arrowheads). (D) Magnified detail from Figure 2C . Profiles N1 and N2 with small clear and dark dense core vesicles are presynaptic to a third small profile (asterisk). In adjacent sections, this profile contained large granular vesicles. Presynaptic structures consist of a bar-shaped density surrounded by small clear vesicles. The synapses may be monadic or dyadic. Scale bar in (D) : 0.5 μm (applies to A–D ).

    Article Snippet: Data Evaluation and Statistics Ultrathin sections were examined and photographed with a transmission electron microscope (EM 109 and EM10C, Zeiss, Oberkochen, Germany).

    Techniques:

    Ultrathin sections of Ishikawa cells. A : Ishikawa cells before treatment with toxin A. Cells grow as monolayers and show apico-basal polarity with nuclei located at the base of the cells and organelles found predominantly in the supranuclear region of the cells. Insert (light microscopy, cross section) shows overview of Ishikawa cells growing as monolayers. B : Lateral cell membranes show tight junctions, adherens junctions and desmosomes in varying combinations, whereas regular junctional complexes consisting of tight junction, adherens junction and desmosomes in apico-basal sequence were rarely seen. C : Ishikawa monolayers after treatment with toxin A. Standard electron microscopy showed no substantial differences between untreated (A) and toxin A-treated cells (C). Me: cell culture medium; N: nucleus; oo: coverslip; *: desmosome; arrow: adherens junction.

    Journal: Journal of Experimental & Clinical Assisted Reproduction

    Article Title: Molecular mechanisms in uterine epithelium during trophoblast binding: the role of small GTPase RhoA in human uterine Ishikawa cells

    doi: 10.1186/1743-1050-2-4

    Figure Lengend Snippet: Ultrathin sections of Ishikawa cells. A : Ishikawa cells before treatment with toxin A. Cells grow as monolayers and show apico-basal polarity with nuclei located at the base of the cells and organelles found predominantly in the supranuclear region of the cells. Insert (light microscopy, cross section) shows overview of Ishikawa cells growing as monolayers. B : Lateral cell membranes show tight junctions, adherens junctions and desmosomes in varying combinations, whereas regular junctional complexes consisting of tight junction, adherens junction and desmosomes in apico-basal sequence were rarely seen. C : Ishikawa monolayers after treatment with toxin A. Standard electron microscopy showed no substantial differences between untreated (A) and toxin A-treated cells (C). Me: cell culture medium; N: nucleus; oo: coverslip; *: desmosome; arrow: adherens junction.

    Article Snippet: Ultrathin sections were mounted on 200-mesh copper grids, double-stained with uranyl acetate and lead citrate and examined with a Zeiss 902 A at 80 kV (Carl Zeiss, Jena, Germany).

    Techniques: Light Microscopy, Sequencing, Electron Microscopy, Cell Culture