ultrathin sections  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Thermo Fisher ultrathin sections
    Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for <t>ultrathin</t> cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P
    Ultrathin Sections, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/Thermo Fisher
    Average 94 stars, based on 230 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    94/100 stars

    Images

    1) Product Images from "Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers"

    Article Title: Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201709055

    Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for ultrathin cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P
    Figure Legend Snippet: Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for ultrathin cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P

    Techniques Used: Western Blot, Expressing, Labeling, Cell Culture, Staining

    2) Product Images from "Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches"

    Article Title: Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/j.1582-4934.2009.00758.x

    ( A ) and ( B ). Electron micrographs on serial ultrathin sections highlight the connections of the ICLC processes (ICLCp) with the basal lamina of the CMP by electron dense nanostructures (arrows). The ICLC process seems to support and guide the basal lamina, which exceeds the cellular profile of the CMP (dotted arrows). ( C ), ( D ) – High magnifications of the areas marked in Fig. 6 point out the close relationship of the ICLCp with the basal lamina of the CMP. Arrows indicate adjoining points.
    Figure Legend Snippet: ( A ) and ( B ). Electron micrographs on serial ultrathin sections highlight the connections of the ICLC processes (ICLCp) with the basal lamina of the CMP by electron dense nanostructures (arrows). The ICLC process seems to support and guide the basal lamina, which exceeds the cellular profile of the CMP (dotted arrows). ( C ), ( D ) – High magnifications of the areas marked in Fig. 6 point out the close relationship of the ICLCp with the basal lamina of the CMP. Arrows indicate adjoining points.

    Techniques Used:

    3) Product Images from "Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin"

    Article Title: Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin

    Journal: Aging Cell

    doi: 10.1111/acel.12493

    Reduced cell cohesiveness in repaired epidermis of aged individuals is accompanied by reduced number of desmosomes. (A) Toluidine blue staining of ultrathin skin sections from representative young (left) and aged (right) skin samples taken 14 days post‐wounding reveals greater intracellular spacing between basal and suprabasal keratinocytes in repaired epidermis of aged vs. young individuals. Scale bar = 50 μm. (B) Intracellular space area normalized to total epidermal area. Quantification was from toluidine blue‐stained samples taken 14 days post‐wounding. N = 3/age group. *: P
    Figure Legend Snippet: Reduced cell cohesiveness in repaired epidermis of aged individuals is accompanied by reduced number of desmosomes. (A) Toluidine blue staining of ultrathin skin sections from representative young (left) and aged (right) skin samples taken 14 days post‐wounding reveals greater intracellular spacing between basal and suprabasal keratinocytes in repaired epidermis of aged vs. young individuals. Scale bar = 50 μm. (B) Intracellular space area normalized to total epidermal area. Quantification was from toluidine blue‐stained samples taken 14 days post‐wounding. N = 3/age group. *: P

    Techniques Used: Staining

    4) Product Images from "A human beta cell line with drug inducible excision of immortalizing transgenes"

    Article Title: A human beta cell line with drug inducible excision of immortalizing transgenes

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2015.09.008

    TEM analysis of treated and untreated EndoC-βH3 . Non-treated and treated EndoC-βH3 cells were analyzed after Epon embedding and ultrathin sectioning. ( A ) Non-treated EndoC-βH3, inset: detail of insulin SGs, ( B ) TAM-treated EndoC-βH3, inset: detail of insulin SGs. Scale bar: 2 μm. Labeling key: Nuc, nucleus; Mito, mitochondria; SG, secretory granules; arrowheads, degradative compartments.
    Figure Legend Snippet: TEM analysis of treated and untreated EndoC-βH3 . Non-treated and treated EndoC-βH3 cells were analyzed after Epon embedding and ultrathin sectioning. ( A ) Non-treated EndoC-βH3, inset: detail of insulin SGs, ( B ) TAM-treated EndoC-βH3, inset: detail of insulin SGs. Scale bar: 2 μm. Labeling key: Nuc, nucleus; Mito, mitochondria; SG, secretory granules; arrowheads, degradative compartments.

    Techniques Used: Transmission Electron Microscopy, Labeling

    5) Product Images from "Ultrastructural Study of Rat Testis Following Conventional Phototherapy during Neonatal Period"

    Article Title: Ultrastructural Study of Rat Testis Following Conventional Phototherapy during Neonatal Period

    Journal: Journal of Microscopy and Ultrastructure

    doi: 10.4103/JMAU.JMAU_17_18

    Transmission electron photomicrographs of ultrathin section of rat's testis showing Sertoli cells (St) with large euchromatic indented nucleus (N) with a prominent nucleolus (nu) and mitochondria (M) in control rat's testis (a); with irregular indented nucleus (N) and nucleolus (nu), dilated endoplasmic reticulum and M with irregular cristae in the phototherapy treated group at 70 days (b); having an irregular indented N, dilated ER in the phototherapy treated group at 100 days (c); appears with indented N with normal M and ER in phototherapy treated group at 130 days (d). BM: Basement membrane. Scale bar: a = b = c = d = 1 μm
    Figure Legend Snippet: Transmission electron photomicrographs of ultrathin section of rat's testis showing Sertoli cells (St) with large euchromatic indented nucleus (N) with a prominent nucleolus (nu) and mitochondria (M) in control rat's testis (a); with irregular indented nucleus (N) and nucleolus (nu), dilated endoplasmic reticulum and M with irregular cristae in the phototherapy treated group at 70 days (b); having an irregular indented N, dilated ER in the phototherapy treated group at 100 days (c); appears with indented N with normal M and ER in phototherapy treated group at 130 days (d). BM: Basement membrane. Scale bar: a = b = c = d = 1 μm

    Techniques Used: Transmission Assay

    Transmission electron photomicrographs of ultrathin section of rat's testis showing spermatogonium lying on the basement membrane, with myoid cell with peripheral heterochromatin in nucleus (N) and normal mitochondria (M) in control group. (a); Sg having dense-clumped marginal chromatin material (*) in the nucleus (N) and vacuolation (V) in cytoplasm in phototherapy treated group at 70 days (b); Sg lying on irregular BM having distorted mitochondria (M) in phototherapy treated group at 100 days (c); with condensed heterochromatin in its nucleus (N) and normal appearing mitochondria in phototherapy treated group at 130 days (d). Scale bar: a = 1 μm; b = c = d = 2 μm
    Figure Legend Snippet: Transmission electron photomicrographs of ultrathin section of rat's testis showing spermatogonium lying on the basement membrane, with myoid cell with peripheral heterochromatin in nucleus (N) and normal mitochondria (M) in control group. (a); Sg having dense-clumped marginal chromatin material (*) in the nucleus (N) and vacuolation (V) in cytoplasm in phototherapy treated group at 70 days (b); Sg lying on irregular BM having distorted mitochondria (M) in phototherapy treated group at 100 days (c); with condensed heterochromatin in its nucleus (N) and normal appearing mitochondria in phototherapy treated group at 130 days (d). Scale bar: a = 1 μm; b = c = d = 2 μm

    Techniques Used: Transmission Assay

    Transmission electron photomicrographs of ultrathin section of rat's testis showing primary spermatocyte with large, rounded nucleus (N) and ovoid mitochondria (M) in control group (a); PSp with clumped dense chromatin material (*) in the margins of the nucleus (N), cytoplasmic vacuoles (V), and swollen mitochondria (M) with irregular cristae in phototherapy treated group at 70 days (b); PSp with vacuoles in cytoplasm (V) and distorted mitochondria (M) with irregular cristae in phototherapy treated group at 100 days (c); the PSp appears as a large, rounded cell with round nucleus (N) containing clumps of heterochromatin scattered all over the nucleoplasm in phototherapy treated group at 130 days (d). Scale bar: A =1 μM; B = c = d = 2 μm
    Figure Legend Snippet: Transmission electron photomicrographs of ultrathin section of rat's testis showing primary spermatocyte with large, rounded nucleus (N) and ovoid mitochondria (M) in control group (a); PSp with clumped dense chromatin material (*) in the margins of the nucleus (N), cytoplasmic vacuoles (V), and swollen mitochondria (M) with irregular cristae in phototherapy treated group at 70 days (b); PSp with vacuoles in cytoplasm (V) and distorted mitochondria (M) with irregular cristae in phototherapy treated group at 100 days (c); the PSp appears as a large, rounded cell with round nucleus (N) containing clumps of heterochromatin scattered all over the nucleoplasm in phototherapy treated group at 130 days (d). Scale bar: A =1 μM; B = c = d = 2 μm

    Techniques Used: Transmission Assay

    Transmission electron photomicrographs of ultrathin section of rat's testis showing spermatid containing large, rounded euchromatic nucleus (N), prominent Golgi apparatus (G) and acrosomal cap at one side of the nucleus and peripherally arranged mitochondria (M) in control group (a); spermatid having an irregular and damaged nuclear membrane (arrowheads), a few damaged mitochondria (M) and electron-dense material (*) in the cytoplasm in the phototherapy treated group at 70 days (b); spermatid with nuclear rupture (arrow), loss of cell boundary, and abnormal acrosome formation in the phototherapy treated group at 100 days (c); spermatid having normal euchromatic nucleus (N) and a prominent acrosomal cap in the phototherapy treated group at 130 days (d). Scale bar: a = b = d = 1 μm; c = 2 μm
    Figure Legend Snippet: Transmission electron photomicrographs of ultrathin section of rat's testis showing spermatid containing large, rounded euchromatic nucleus (N), prominent Golgi apparatus (G) and acrosomal cap at one side of the nucleus and peripherally arranged mitochondria (M) in control group (a); spermatid having an irregular and damaged nuclear membrane (arrowheads), a few damaged mitochondria (M) and electron-dense material (*) in the cytoplasm in the phototherapy treated group at 70 days (b); spermatid with nuclear rupture (arrow), loss of cell boundary, and abnormal acrosome formation in the phototherapy treated group at 100 days (c); spermatid having normal euchromatic nucleus (N) and a prominent acrosomal cap in the phototherapy treated group at 130 days (d). Scale bar: a = b = d = 1 μm; c = 2 μm

    Techniques Used: Transmission Assay

    A transmission electron photomicrograph of an ultrathin section of a control rat's testis at 70 days showing basement membrane surrounding the seminiferous tubule. Sg: Spermatogonium, PSp: Primary spermatocyte, Sd: Spermatid, AC: Acrosomal cap, M: Mitochondria, ER: Endoplasmic reticulum. Scale bar-5 μm
    Figure Legend Snippet: A transmission electron photomicrograph of an ultrathin section of a control rat's testis at 70 days showing basement membrane surrounding the seminiferous tubule. Sg: Spermatogonium, PSp: Primary spermatocyte, Sd: Spermatid, AC: Acrosomal cap, M: Mitochondria, ER: Endoplasmic reticulum. Scale bar-5 μm

    Techniques Used: Transmission Assay

    6) Product Images from "Expression and Role of VEGF-A in the Ciliary Body"

    Article Title: Expression and Role of VEGF-A in the Ciliary Body

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.12-10098

    VEGF-A neutralization leads to ciliary capillary thrombosis. Richardson stain of epoxy embedded ultrathin sections of eyes from ( A , B ) Ad-null and ( C , D ) Ad-sFlt1–expressing mice at day 28 post-infection revealed numerous microthrombi ( white arrowheads
    Figure Legend Snippet: VEGF-A neutralization leads to ciliary capillary thrombosis. Richardson stain of epoxy embedded ultrathin sections of eyes from ( A , B ) Ad-null and ( C , D ) Ad-sFlt1–expressing mice at day 28 post-infection revealed numerous microthrombi ( white arrowheads

    Techniques Used: Neutralization, Staining, Expressing, Mouse Assay, Infection

    7) Product Images from "Imaging Transient Blood Vessel Fusion Events in Zebrafish by Correlative Volume Electron Microscopy"

    Article Title: Imaging Transient Blood Vessel Fusion Events in Zebrafish by Correlative Volume Electron Microscopy

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007716

    Preliminary studies of anastomosis in zebrafish. (A) Longitudinal diagram of 48 hpf zebrafish embryo, with neural tube (NT, green), notochord (N, turquoise), posterior cardinal vein (PCV, dark blue), dorsal aorta (DA, red), intersegmental vessels (ISVs, dark blue and red according to origin) and dorsal lateral anastomotic vessel (DLAV) shown in the trunk/tail region. (B) Cross section of zebrafish trunk; muscle blocks (M, pink) and yolk tube (YT, yellow) are also shown. (C) In vivo live confocal imaging of the anastomotic process showing two adjacent ISVs sprouting from the dorsal aorta and extending filopodia which eventually fuse to form the DLAV. (D) Ultrathin TEM sections give a general overview of the fish anatomy but it is not possible to identify the ISVs or DLAV as they do not have the lumen characteristic of blood vessels at this point. Bar (C–C′′′) 10 µm, (D) 10 µm.
    Figure Legend Snippet: Preliminary studies of anastomosis in zebrafish. (A) Longitudinal diagram of 48 hpf zebrafish embryo, with neural tube (NT, green), notochord (N, turquoise), posterior cardinal vein (PCV, dark blue), dorsal aorta (DA, red), intersegmental vessels (ISVs, dark blue and red according to origin) and dorsal lateral anastomotic vessel (DLAV) shown in the trunk/tail region. (B) Cross section of zebrafish trunk; muscle blocks (M, pink) and yolk tube (YT, yellow) are also shown. (C) In vivo live confocal imaging of the anastomotic process showing two adjacent ISVs sprouting from the dorsal aorta and extending filopodia which eventually fuse to form the DLAV. (D) Ultrathin TEM sections give a general overview of the fish anatomy but it is not possible to identify the ISVs or DLAV as they do not have the lumen characteristic of blood vessels at this point. Bar (C–C′′′) 10 µm, (D) 10 µm.

    Techniques Used: In Vivo, Imaging, Transmission Electron Microscopy, Fluorescence In Situ Hybridization

    8) Product Images from "Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers"

    Article Title: Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201709055

    Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for ultrathin cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P
    Figure Legend Snippet: Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for ultrathin cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P

    Techniques Used: Western Blot, Expressing, Labeling, Cell Culture, Staining

    9) Product Images from "Denatured H-ferritin subunit is a major constituent of haemosiderin in the liver of patients with iron overload"

    Article Title: Denatured H-ferritin subunit is a major constituent of haemosiderin in the liver of patients with iron overload

    Journal: Gut

    doi:

    Ultrastructural features and distribution of ferritin subunits in hepatocytes of a normal liver. Ferritin subunits in the ultrathin sections were immunohistochemically stained using antiferritin antibodies LF03 (A), rH02 (B), M3 (C), and HS-59 (D), followed by addition of a rabbit antimouse IgG coupled with colloidal gold particles. Bar represents 0.5 μm.
    Figure Legend Snippet: Ultrastructural features and distribution of ferritin subunits in hepatocytes of a normal liver. Ferritin subunits in the ultrathin sections were immunohistochemically stained using antiferritin antibodies LF03 (A), rH02 (B), M3 (C), and HS-59 (D), followed by addition of a rabbit antimouse IgG coupled with colloidal gold particles. Bar represents 0.5 μm.

    Techniques Used: Staining

    10) Product Images from "Zika virus replication in the mosquito Culex quinquefasciatus in Brazil"

    Article Title: Zika virus replication in the mosquito Culex quinquefasciatus in Brazil

    Journal: Emerging Microbes & Infections

    doi: 10.1038/emi.2017.59

    ( A and B ) Ultrathin sections of an uninfected Cx. quinquefasciatus salivary gland. ( A ) This micrograph shows the electrodense content of the apical cavity ( A and C ) with membrane projections that extend from the wall. ( B ) Uninfected acinar salivary gland cell showing the Nu, ER and Mi. ( C and D ) Cytopathic effects of salivary glands cells infected with ZIKV showing several patches of TPM, dER and a PhV. Cell cytoplasm, Cyt; distended endoplasmic reticula, dER; endoplasmic reticulum, ER; mitochondria, Mi; nucleus, Nu; phagolysosome-like vacuole, PhV; thread-like center, TC; tubular proliferated membrane, TPM; Zika virus, ZIKV.
    Figure Legend Snippet: ( A and B ) Ultrathin sections of an uninfected Cx. quinquefasciatus salivary gland. ( A ) This micrograph shows the electrodense content of the apical cavity ( A and C ) with membrane projections that extend from the wall. ( B ) Uninfected acinar salivary gland cell showing the Nu, ER and Mi. ( C and D ) Cytopathic effects of salivary glands cells infected with ZIKV showing several patches of TPM, dER and a PhV. Cell cytoplasm, Cyt; distended endoplasmic reticula, dER; endoplasmic reticulum, ER; mitochondria, Mi; nucleus, Nu; phagolysosome-like vacuole, PhV; thread-like center, TC; tubular proliferated membrane, TPM; Zika virus, ZIKV.

    Techniques Used: Infection

    11) Product Images from "Ultrastructural Study of Rat Testis Following Conventional Phototherapy during Neonatal Period"

    Article Title: Ultrastructural Study of Rat Testis Following Conventional Phototherapy during Neonatal Period

    Journal: Journal of Microscopy and Ultrastructure

    doi: 10.4103/JMAU.JMAU_17_18

    Transmission electron photomicrographs of ultrathin section of rat's testis showing Sertoli cells (St) with large euchromatic indented nucleus (N) with a prominent nucleolus (nu) and mitochondria (M) in control rat's testis (a); with irregular indented nucleus (N) and nucleolus (nu), dilated endoplasmic reticulum and M with irregular cristae in the phototherapy treated group at 70 days (b); having an irregular indented N, dilated ER in the phototherapy treated group at 100 days (c); appears with indented N with normal M and ER in phototherapy treated group at 130 days (d). BM: Basement membrane. Scale bar: a = b = c = d = 1 μm
    Figure Legend Snippet: Transmission electron photomicrographs of ultrathin section of rat's testis showing Sertoli cells (St) with large euchromatic indented nucleus (N) with a prominent nucleolus (nu) and mitochondria (M) in control rat's testis (a); with irregular indented nucleus (N) and nucleolus (nu), dilated endoplasmic reticulum and M with irregular cristae in the phototherapy treated group at 70 days (b); having an irregular indented N, dilated ER in the phototherapy treated group at 100 days (c); appears with indented N with normal M and ER in phototherapy treated group at 130 days (d). BM: Basement membrane. Scale bar: a = b = c = d = 1 μm

    Techniques Used: Transmission Assay

    Transmission electron photomicrographs of ultrathin section of rat's testis showing spermatogonium lying on the basement membrane, with myoid cell with peripheral heterochromatin in nucleus (N) and normal mitochondria (M) in control group. (a); Sg having dense-clumped marginal chromatin material (*) in the nucleus (N) and vacuolation (V) in cytoplasm in phototherapy treated group at 70 days (b); Sg lying on irregular BM having distorted mitochondria (M) in phototherapy treated group at 100 days (c); with condensed heterochromatin in its nucleus (N) and normal appearing mitochondria in phototherapy treated group at 130 days (d). Scale bar: a = 1 μm; b = c = d = 2 μm
    Figure Legend Snippet: Transmission electron photomicrographs of ultrathin section of rat's testis showing spermatogonium lying on the basement membrane, with myoid cell with peripheral heterochromatin in nucleus (N) and normal mitochondria (M) in control group. (a); Sg having dense-clumped marginal chromatin material (*) in the nucleus (N) and vacuolation (V) in cytoplasm in phototherapy treated group at 70 days (b); Sg lying on irregular BM having distorted mitochondria (M) in phototherapy treated group at 100 days (c); with condensed heterochromatin in its nucleus (N) and normal appearing mitochondria in phototherapy treated group at 130 days (d). Scale bar: a = 1 μm; b = c = d = 2 μm

    Techniques Used: Transmission Assay

    Transmission electron photomicrographs of ultrathin section of rat's testis showing primary spermatocyte with large, rounded nucleus (N) and ovoid mitochondria (M) in control group (a); PSp with clumped dense chromatin material (*) in the margins of the nucleus (N), cytoplasmic vacuoles (V), and swollen mitochondria (M) with irregular cristae in phototherapy treated group at 70 days (b); PSp with vacuoles in cytoplasm (V) and distorted mitochondria (M) with irregular cristae in phototherapy treated group at 100 days (c); the PSp appears as a large, rounded cell with round nucleus (N) containing clumps of heterochromatin scattered all over the nucleoplasm in phototherapy treated group at 130 days (d). Scale bar: A =1 μM; B = c = d = 2 μm
    Figure Legend Snippet: Transmission electron photomicrographs of ultrathin section of rat's testis showing primary spermatocyte with large, rounded nucleus (N) and ovoid mitochondria (M) in control group (a); PSp with clumped dense chromatin material (*) in the margins of the nucleus (N), cytoplasmic vacuoles (V), and swollen mitochondria (M) with irregular cristae in phototherapy treated group at 70 days (b); PSp with vacuoles in cytoplasm (V) and distorted mitochondria (M) with irregular cristae in phototherapy treated group at 100 days (c); the PSp appears as a large, rounded cell with round nucleus (N) containing clumps of heterochromatin scattered all over the nucleoplasm in phototherapy treated group at 130 days (d). Scale bar: A =1 μM; B = c = d = 2 μm

    Techniques Used: Transmission Assay

    Transmission electron photomicrographs of ultrathin section of rat's testis showing spermatid containing large, rounded euchromatic nucleus (N), prominent Golgi apparatus (G) and acrosomal cap at one side of the nucleus and peripherally arranged mitochondria (M) in control group (a); spermatid having an irregular and damaged nuclear membrane (arrowheads), a few damaged mitochondria (M) and electron-dense material (*) in the cytoplasm in the phototherapy treated group at 70 days (b); spermatid with nuclear rupture (arrow), loss of cell boundary, and abnormal acrosome formation in the phototherapy treated group at 100 days (c); spermatid having normal euchromatic nucleus (N) and a prominent acrosomal cap in the phototherapy treated group at 130 days (d). Scale bar: a = b = d = 1 μm; c = 2 μm
    Figure Legend Snippet: Transmission electron photomicrographs of ultrathin section of rat's testis showing spermatid containing large, rounded euchromatic nucleus (N), prominent Golgi apparatus (G) and acrosomal cap at one side of the nucleus and peripherally arranged mitochondria (M) in control group (a); spermatid having an irregular and damaged nuclear membrane (arrowheads), a few damaged mitochondria (M) and electron-dense material (*) in the cytoplasm in the phototherapy treated group at 70 days (b); spermatid with nuclear rupture (arrow), loss of cell boundary, and abnormal acrosome formation in the phototherapy treated group at 100 days (c); spermatid having normal euchromatic nucleus (N) and a prominent acrosomal cap in the phototherapy treated group at 130 days (d). Scale bar: a = b = d = 1 μm; c = 2 μm

    Techniques Used: Transmission Assay

    A transmission electron photomicrograph of an ultrathin section of a control rat's testis at 70 days showing basement membrane surrounding the seminiferous tubule. Sg: Spermatogonium, PSp: Primary spermatocyte, Sd: Spermatid, AC: Acrosomal cap, M: Mitochondria, ER: Endoplasmic reticulum. Scale bar-5 μm
    Figure Legend Snippet: A transmission electron photomicrograph of an ultrathin section of a control rat's testis at 70 days showing basement membrane surrounding the seminiferous tubule. Sg: Spermatogonium, PSp: Primary spermatocyte, Sd: Spermatid, AC: Acrosomal cap, M: Mitochondria, ER: Endoplasmic reticulum. Scale bar-5 μm

    Techniques Used: Transmission Assay

    12) Product Images from "Matrix Metalloproteinase-1 and Acid Phosphatase in the Degradation of the Lamina Propria of Eruptive Pathway of Rat Molars"

    Article Title: Matrix Metalloproteinase-1 and Acid Phosphatase in the Degradation of the Lamina Propria of Eruptive Pathway of Rat Molars

    Journal: Cells

    doi: 10.3390/cells7110206

    Electron micrographs of portions of lamina propria of the eruptive pathway of first molars of 9- ( A ), 13- ( B and F ), 16- ( C – E ) day-old rats. A – E —portions of the eruptive pathway of first molars incubated for acid phosphatase reaction. A —a round-shaped cell exhibits electron-opaque deposits in the lysosomes (L). In high magnification (inset), conspicuous electron-opaque deposits (arrows)—reaction product of the acid phosphatase activity—is irregularly distributed throughout the lysosome. N, nucleus. Bars: 5 µm and 0.2 µm (inset). In B , several lysosomes (L) exhibiting electron-opaque deposits (arrows) are observed in the cytoplasm of a macrophage. N, nucleus; CF, collagen fibrils. Bar: 1.5 µm. C —an irregular cell (C) with large vacuole (V) is surrounding partially a heterogeneous material (M). Electron-opaque deposits (arrows) are observed in the lysosomes (L) and in the periphery of a small vacuole (SV). The inset shows conspicuous electron-opaque deposits almost entirely filling a lysosome (L). rER, rough endoplasmic reticulum. Bars: 4 µm and 0.3 µm (inset). D —high magnification of the large vacuole (V) observed in the superior portion of the Figure 9 C. Fine products of the reaction to acid phosphatase (arrows) are observed on the partially digested material (M). Granular electron-opaque deposits (arrows) are also seen in the periphery of a small vacuole (SV). Bar: 1 µm. Figure 9 E —a large lysosome (L) exhibiting acid phosphatase-positive deposits (arrows) intermingled with heterogeneous material is present in the fibroblast. Bar: 0.5 µm. F —ultrathin section of a portion of the eruptive pathway incubated in substrate-free medium (negative control). No reaction product is observed in the lysosome (L) of cellular portions (CP) in the lamina propria (LP). Bar: 1.5 µm.
    Figure Legend Snippet: Electron micrographs of portions of lamina propria of the eruptive pathway of first molars of 9- ( A ), 13- ( B and F ), 16- ( C – E ) day-old rats. A – E —portions of the eruptive pathway of first molars incubated for acid phosphatase reaction. A —a round-shaped cell exhibits electron-opaque deposits in the lysosomes (L). In high magnification (inset), conspicuous electron-opaque deposits (arrows)—reaction product of the acid phosphatase activity—is irregularly distributed throughout the lysosome. N, nucleus. Bars: 5 µm and 0.2 µm (inset). In B , several lysosomes (L) exhibiting electron-opaque deposits (arrows) are observed in the cytoplasm of a macrophage. N, nucleus; CF, collagen fibrils. Bar: 1.5 µm. C —an irregular cell (C) with large vacuole (V) is surrounding partially a heterogeneous material (M). Electron-opaque deposits (arrows) are observed in the lysosomes (L) and in the periphery of a small vacuole (SV). The inset shows conspicuous electron-opaque deposits almost entirely filling a lysosome (L). rER, rough endoplasmic reticulum. Bars: 4 µm and 0.3 µm (inset). D —high magnification of the large vacuole (V) observed in the superior portion of the Figure 9 C. Fine products of the reaction to acid phosphatase (arrows) are observed on the partially digested material (M). Granular electron-opaque deposits (arrows) are also seen in the periphery of a small vacuole (SV). Bar: 1 µm. Figure 9 E —a large lysosome (L) exhibiting acid phosphatase-positive deposits (arrows) intermingled with heterogeneous material is present in the fibroblast. Bar: 0.5 µm. F —ultrathin section of a portion of the eruptive pathway incubated in substrate-free medium (negative control). No reaction product is observed in the lysosome (L) of cellular portions (CP) in the lamina propria (LP). Bar: 1.5 µm.

    Techniques Used: Incubation, Activity Assay, Negative Control

    Related Articles

    Electron Microscopy:

    Article Title: Expression and Role of VEGF-A in the Ciliary Body
    Article Snippet: .. Ultrathin sections were treated with uranyl acetate and visualized by transmission electron microscopy (TEM) using a transmission electron microscope (Tecnai G2 Spirit BioTwin; FEI Company, Hillsboro, OR). .. VEGFR2 was localized by fluorescent immunohistochemistry.

    Article Title: Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin
    Article Snippet: .. Ultrathin sections were either stained with toluidine blue and mounted in Permount (Thermo Fisher Scientific) for light microscopy, or transferred onto a grid for transmission electron microscopy (performed on a Phillips CM‐100 (Eindhoven, The Netherlands), accessed at the Microscopy and Image‐Analysis Laboratory core facility). .. Charts and statistics Charts were generated with Microsoft Excel 2010 or graphpad prism 6.0 (La Jolla, CA) and assembled in Photoshop CC .

    Microscopy:

    Article Title: Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers
    Article Snippet: .. Ultrathin sections were observed at 80 kV transmission electron microscope (Tecnai Spirit G2; Thermo Fisher Scientific) equipped with a QUEMESA CCD camera (EMSIS) using iTEM software (EMSIS). .. For ultrathin cryosections and immunogold labeling, MNT-1 cells were grown on six-well plates and fixed with 2% PFA/0.2% glutaraldehyde/0.1 M phosphate buffer.

    Article Title: Expression and Role of VEGF-A in the Ciliary Body
    Article Snippet: .. Ultrathin sections were treated with uranyl acetate and visualized by transmission electron microscopy (TEM) using a transmission electron microscope (Tecnai G2 Spirit BioTwin; FEI Company, Hillsboro, OR). .. VEGFR2 was localized by fluorescent immunohistochemistry.

    Article Title: A human beta cell line with drug inducible excision of immortalizing transgenes
    Article Snippet: .. Ultrathin sections with a thickness of 70 nm were cut on a Leica EM6 ultramicrotome and imaged using a Tecnai 12 Biotwin Transmission Electron Microscope (FEI Company, Hillsboro, OR, USA) with a bottom-mount 2 Å∼2K F214 CCD camera (TVIPS, Gauting, Germany). ..

    Article Title: Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin
    Article Snippet: .. Ultrathin sections were either stained with toluidine blue and mounted in Permount (Thermo Fisher Scientific) for light microscopy, or transferred onto a grid for transmission electron microscopy (performed on a Phillips CM‐100 (Eindhoven, The Netherlands), accessed at the Microscopy and Image‐Analysis Laboratory core facility). .. Charts and statistics Charts were generated with Microsoft Excel 2010 or graphpad prism 6.0 (La Jolla, CA) and assembled in Photoshop CC .

    Light Microscopy:

    Article Title: Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin
    Article Snippet: .. Ultrathin sections were either stained with toluidine blue and mounted in Permount (Thermo Fisher Scientific) for light microscopy, or transferred onto a grid for transmission electron microscopy (performed on a Phillips CM‐100 (Eindhoven, The Netherlands), accessed at the Microscopy and Image‐Analysis Laboratory core facility). .. Charts and statistics Charts were generated with Microsoft Excel 2010 or graphpad prism 6.0 (La Jolla, CA) and assembled in Photoshop CC .

    Transmission Assay:

    Article Title: Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers
    Article Snippet: .. Ultrathin sections were observed at 80 kV transmission electron microscope (Tecnai Spirit G2; Thermo Fisher Scientific) equipped with a QUEMESA CCD camera (EMSIS) using iTEM software (EMSIS). .. For ultrathin cryosections and immunogold labeling, MNT-1 cells were grown on six-well plates and fixed with 2% PFA/0.2% glutaraldehyde/0.1 M phosphate buffer.

    Article Title: Expression and Role of VEGF-A in the Ciliary Body
    Article Snippet: .. Ultrathin sections were treated with uranyl acetate and visualized by transmission electron microscopy (TEM) using a transmission electron microscope (Tecnai G2 Spirit BioTwin; FEI Company, Hillsboro, OR). .. VEGFR2 was localized by fluorescent immunohistochemistry.

    Article Title: A human beta cell line with drug inducible excision of immortalizing transgenes
    Article Snippet: .. Ultrathin sections with a thickness of 70 nm were cut on a Leica EM6 ultramicrotome and imaged using a Tecnai 12 Biotwin Transmission Electron Microscope (FEI Company, Hillsboro, OR, USA) with a bottom-mount 2 Å∼2K F214 CCD camera (TVIPS, Gauting, Germany). ..

    Article Title: Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin
    Article Snippet: .. Ultrathin sections were either stained with toluidine blue and mounted in Permount (Thermo Fisher Scientific) for light microscopy, or transferred onto a grid for transmission electron microscopy (performed on a Phillips CM‐100 (Eindhoven, The Netherlands), accessed at the Microscopy and Image‐Analysis Laboratory core facility). .. Charts and statistics Charts were generated with Microsoft Excel 2010 or graphpad prism 6.0 (La Jolla, CA) and assembled in Photoshop CC .

    Staining:

    Article Title: Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin
    Article Snippet: .. Ultrathin sections were either stained with toluidine blue and mounted in Permount (Thermo Fisher Scientific) for light microscopy, or transferred onto a grid for transmission electron microscopy (performed on a Phillips CM‐100 (Eindhoven, The Netherlands), accessed at the Microscopy and Image‐Analysis Laboratory core facility). .. Charts and statistics Charts were generated with Microsoft Excel 2010 or graphpad prism 6.0 (La Jolla, CA) and assembled in Photoshop CC .

    Article Title: Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches
    Article Snippet: .. Ultrathin sections stained with uranyl acetate and Reynolds's lead citrate solutions were examined using a Morgagni 286 TEM (FEI Company, Eindhoven, The Nederlands) at 60 kV. .. Digital electron micrographs were recorded with a MegaView III CCD using iTEM-SIS software (Olympus, Soft Imaging System GmbH, Germany).

    Article Title: Ultrastructural Study of Rat Testis Following Conventional Phototherapy during Neonatal Period
    Article Snippet: .. Thereafter, ultrathin sections (70–80 nm, silver to gold sections) were cut and stained with alcoholic uranyl acetate and alkaline Pb citrate and observed under a Morgagni 268D TEM (FEI Company, the Netherlands) at an operating voltage 80 kV. .. Images were digitally acquired by a CCD camera (Megaview III, FEI Company) using iTEM software (Soft Imaging System, Münster, Germany) attached to the microscope.

    Transmission Electron Microscopy:

    Article Title: Expression and Role of VEGF-A in the Ciliary Body
    Article Snippet: .. Ultrathin sections were treated with uranyl acetate and visualized by transmission electron microscopy (TEM) using a transmission electron microscope (Tecnai G2 Spirit BioTwin; FEI Company, Hillsboro, OR). .. VEGFR2 was localized by fluorescent immunohistochemistry.

    Article Title: Imaging Transient Blood Vessel Fusion Events in Zebrafish by Correlative Volume Electron Microscopy
    Article Snippet: .. Ultrathin sections of 80 nm were cut, post-stained with lead citrate and viewed in a Tecnai Spirit Biotwin 120 keV TEM (FEI Company). .. Images were captured using Ultrascan and Orius CCDs (Gatan Inc.) with TIA (Tecnai Imaging and Analysis, FEI Company) and Digital Micrograph software (Gatan Inc.) respectively.

    Article Title: Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches
    Article Snippet: .. Ultrathin sections stained with uranyl acetate and Reynolds's lead citrate solutions were examined using a Morgagni 286 TEM (FEI Company, Eindhoven, The Nederlands) at 60 kV. .. Digital electron micrographs were recorded with a MegaView III CCD using iTEM-SIS software (Olympus, Soft Imaging System GmbH, Germany).

    Article Title: Ultrastructural Study of Rat Testis Following Conventional Phototherapy during Neonatal Period
    Article Snippet: .. Thereafter, ultrathin sections (70–80 nm, silver to gold sections) were cut and stained with alcoholic uranyl acetate and alkaline Pb citrate and observed under a Morgagni 268D TEM (FEI Company, the Netherlands) at an operating voltage 80 kV. .. Images were digitally acquired by a CCD camera (Megaview III, FEI Company) using iTEM software (Soft Imaging System, Münster, Germany) attached to the microscope.

    Software:

    Article Title: Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers
    Article Snippet: .. Ultrathin sections were observed at 80 kV transmission electron microscope (Tecnai Spirit G2; Thermo Fisher Scientific) equipped with a QUEMESA CCD camera (EMSIS) using iTEM software (EMSIS). .. For ultrathin cryosections and immunogold labeling, MNT-1 cells were grown on six-well plates and fixed with 2% PFA/0.2% glutaraldehyde/0.1 M phosphate buffer.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher ultrathin sections
    Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for <t>ultrathin</t> cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P
    Ultrathin Sections, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/Thermo Fisher
    Average 94 stars, based on 230 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for ultrathin cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P

    Journal: The Journal of Cell Biology

    Article Title: Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers

    doi: 10.1083/jcb.201709055

    Figure Lengend Snippet: Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for ultrathin cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P

    Article Snippet: Ultrathin sections were observed at 80 kV transmission electron microscope (Tecnai Spirit G2; Thermo Fisher Scientific) equipped with a QUEMESA CCD camera (EMSIS) using iTEM software (EMSIS).

    Techniques: Western Blot, Expressing, Labeling, Cell Culture, Staining

    Reduced cell cohesiveness in repaired epidermis of aged individuals is accompanied by reduced number of desmosomes. (A) Toluidine blue staining of ultrathin skin sections from representative young (left) and aged (right) skin samples taken 14 days post‐wounding reveals greater intracellular spacing between basal and suprabasal keratinocytes in repaired epidermis of aged vs. young individuals. Scale bar = 50 μm. (B) Intracellular space area normalized to total epidermal area. Quantification was from toluidine blue‐stained samples taken 14 days post‐wounding. N = 3/age group. *: P

    Journal: Aging Cell

    Article Title: Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin

    doi: 10.1111/acel.12493

    Figure Lengend Snippet: Reduced cell cohesiveness in repaired epidermis of aged individuals is accompanied by reduced number of desmosomes. (A) Toluidine blue staining of ultrathin skin sections from representative young (left) and aged (right) skin samples taken 14 days post‐wounding reveals greater intracellular spacing between basal and suprabasal keratinocytes in repaired epidermis of aged vs. young individuals. Scale bar = 50 μm. (B) Intracellular space area normalized to total epidermal area. Quantification was from toluidine blue‐stained samples taken 14 days post‐wounding. N = 3/age group. *: P

    Article Snippet: Ultrathin sections were either stained with toluidine blue and mounted in Permount (Thermo Fisher Scientific) for light microscopy, or transferred onto a grid for transmission electron microscopy (performed on a Phillips CM‐100 (Eindhoven, The Netherlands), accessed at the Microscopy and Image‐Analysis Laboratory core facility).

    Techniques: Staining

    Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for ultrathin cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P

    Journal: The Journal of Cell Biology

    Article Title: Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers

    doi: 10.1083/jcb.201709055

    Figure Lengend Snippet: Melanin production, cargo export, melanosome secretion, and transfer rely on Myo6, WASH, or optineurin. (A) Western blot of total lysates of control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (B) Intracellular melanin estimation of control-, Myo6-, or WASH1-depleted MNT-1 cells ( n = 8 independent experiments; normalized to control). (C) Western blot of melanosome-enriched (Mel) fractions from control-, Myo6-, or WASH1-depleted MNT-1 cells probed with respective antibodies. (D) Quantification of TYRP1 and VAMP7 protein expression levels on Mel fractions in C and normalized to control ( n = 2 independent experiments). (E) GFP-VAMP7–expressing MNT-1 cells treated with siCtrl, siMyo6, or siWASH1 were processed for ultrathin cryosectioning and double immunogold labeled for GFP (PAG 10 nm, arrowheads) and TYRP1 (PAG 15 nm). (F and G) Percentage of GFP-VAMP7 + melanosome per n cell on section (F) and relative number of gold particles per GFP-VAMP7 + melanosome (G; siCtrl, n = 7; siMyo6, n = 6; siWASH1, n = 7). (H) Ratio of extracellular to intracellular melanin content of control-, Myo6-, or WASH1-depleted MNT-1 cells treated with 30 µM forskolin ( n = 3 independent experiments; normalized to control). (I) NHMs treated with control or Myo6 siRNAs were co-cultured with NHKs. Staining for HMB45 (red) or EGFR (green) identify melanin and NHM or NHK respectively. HMB45 staining within NHK (arrowheads) correspond to secreted and transferred melanin. (J and K) Quantification of the percentage of NHKs positive for at least one HMB45 + spot (J; n = 3 independent experiments) and of the mean HMB45 intensity per stained ( n ) NHK (K) when co-cultured with siCtrl, siMyo6, siWASH1 or siOptn NHM (siCtrl, n = 167; siMyo6, n = 147; siWASH1, n = 148; siOptn, n = 123). (L) Working model illustrating the roles of Myo6, optineurin, F-actin, WASH, Arp2/3, and BLOC-3 complexes in the recycling pathway from melanosomes. Molecular mass is in kilodaltons. Data are presented as the mean ± SEM. Bars: (E) 1 µm; (I) 10 µm. ****, P

    Article Snippet: Ultrathin sections were observed at 80 kV transmission electron microscope (Tecnai Spirit G2; Thermo Fisher Scientific) equipped with a QUEMESA CCD camera (EMSIS) using iTEM software (EMSIS).

    Techniques: Western Blot, Expressing, Labeling, Cell Culture, Staining