Structured Review

Philips Healthcare ultrathin sections
Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and <t>ultrathin</t> sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.
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1) Product Images from "Fiberless Recombinant Adenoviruses: Virus Maturation and Infectivity in the Absence of Fiber"

Article Title: Fiberless Recombinant Adenoviruses: Virus Maturation and Infectivity in the Absence of Fiber

Journal: Journal of Virology

doi:

Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and ultrathin sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.
Figure Legend Snippet: Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and ultrathin sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.

Techniques Used: Electron Microscopy, Infection, Produced, Staining

2) Product Images from "GRIM-19, a Cell Death Regulatory Protein, Is Essential for Assembly and Function of Mitochondrial Complex I"

Article Title: GRIM-19, a Cell Death Regulatory Protein, Is Essential for Assembly and Function of Mitochondrial Complex I

Journal: Molecular and Cellular Biology

doi: 10.1128/MCB.24.19.8447-8456.2004

Abnormal morphology and cellular distribution of mitochondria in the mutant blastocysts. Ultrathin sections of the blastocysts were examined with a transmission electron microscope. The mitochondria of the normal (A and C) and the mutant (B and D) cells are indicated by arrows. Scale bar = 1 μm.
Figure Legend Snippet: Abnormal morphology and cellular distribution of mitochondria in the mutant blastocysts. Ultrathin sections of the blastocysts were examined with a transmission electron microscope. The mitochondria of the normal (A and C) and the mutant (B and D) cells are indicated by arrows. Scale bar = 1 μm.

Techniques Used: Mutagenesis, Transmission Assay, Microscopy

3) Product Images from "DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles"

Article Title: DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles

Journal: Journal of Virology

doi:

Electron microscopy of purified virus particles. Particles purified by sucrose gradient centrifugation from HeLa cells infected with WR or vA32i in the absence of IPTG were diluted, collected by high-speed centrifugation, fixed in glutaraldehyde, and embedded in Epon. Ultrathin sections were examined by electron microscopy.
Figure Legend Snippet: Electron microscopy of purified virus particles. Particles purified by sucrose gradient centrifugation from HeLa cells infected with WR or vA32i in the absence of IPTG were diluted, collected by high-speed centrifugation, fixed in glutaraldehyde, and embedded in Epon. Ultrathin sections were examined by electron microscopy.

Techniques Used: Electron Microscopy, Purification, Gradient Centrifugation, Infection, Centrifugation

Morphogenesis of mutant viruses. BS-C-1 cells were infected with vA32/A32i (A and C) or vA32i (B and D) in the absence of IPTG. After 24 h, the cells were fixed in glutaraldehyde and embedded in Epon, and then ultrathin sections were prepared for electron microscopy. Cr, crescents; Nu, nucleoids; IMV, intracellular mature virions; IV, immature virions; DIV, dense immature virions.
Figure Legend Snippet: Morphogenesis of mutant viruses. BS-C-1 cells were infected with vA32/A32i (A and C) or vA32i (B and D) in the absence of IPTG. After 24 h, the cells were fixed in glutaraldehyde and embedded in Epon, and then ultrathin sections were prepared for electron microscopy. Cr, crescents; Nu, nucleoids; IMV, intracellular mature virions; IV, immature virions; DIV, dense immature virions.

Techniques Used: Mutagenesis, Infection, Electron Microscopy

4) Product Images from "Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots"

Article Title: Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots

Journal: Microbial biotechnology

doi: 10.1111/j.1751-7915.2009.00101.x

Transmission electron micrographs of ultrathin sections of knots induced by P. savastanoi pv. savastanoi NCPPB‐3335‐GFP at 35 dpi on in vitro olive plants. A. Ultrastructure of knot tissue showing parenchymatic‐like cells containing a fibrillar cytoplasm and irregular cell wall thickenings (arrowhead). Bacterial cells were visualized at intercellular spaces (black arrow). B. Pseudomonas savastanoi pv. savastanoi cells (black arrow) localized at the intercellular space of two host cells. A condensed cytoplasm (asterisk) and a degraded middle lamella are shown. C. Bacterial cells (black arrow) in contact with a primary cell wall in the process of degradation (arrowhead). D. Abnormal cell wall accumulations of host cells in close contact with degenerated cytoplasm (asterisks) and pathogen cells (black arrow). E. Rod‐shaped (black arrow) and irregular (open arrow) bacterial cells colonizing the extracellular space of a host cell showing degenerated organelles (asterisks). F. Group of P. savastanoi pv. savastanoi cells within a degenerated host cell. Bacterial cells are seen surrounded by an electrolucent halo and immersed in a fibroreticular matrix. G. Detail of (F), high‐magnification image of the bacterial surface releasing outer‐membrane vesicles (arrowheads). H. Detail of the fusion of outer‐membrane vesicles (arrowhead) to the fibroreticular matrix.
Figure Legend Snippet: Transmission electron micrographs of ultrathin sections of knots induced by P. savastanoi pv. savastanoi NCPPB‐3335‐GFP at 35 dpi on in vitro olive plants. A. Ultrastructure of knot tissue showing parenchymatic‐like cells containing a fibrillar cytoplasm and irregular cell wall thickenings (arrowhead). Bacterial cells were visualized at intercellular spaces (black arrow). B. Pseudomonas savastanoi pv. savastanoi cells (black arrow) localized at the intercellular space of two host cells. A condensed cytoplasm (asterisk) and a degraded middle lamella are shown. C. Bacterial cells (black arrow) in contact with a primary cell wall in the process of degradation (arrowhead). D. Abnormal cell wall accumulations of host cells in close contact with degenerated cytoplasm (asterisks) and pathogen cells (black arrow). E. Rod‐shaped (black arrow) and irregular (open arrow) bacterial cells colonizing the extracellular space of a host cell showing degenerated organelles (asterisks). F. Group of P. savastanoi pv. savastanoi cells within a degenerated host cell. Bacterial cells are seen surrounded by an electrolucent halo and immersed in a fibroreticular matrix. G. Detail of (F), high‐magnification image of the bacterial surface releasing outer‐membrane vesicles (arrowheads). H. Detail of the fusion of outer‐membrane vesicles (arrowhead) to the fibroreticular matrix.

Techniques Used: Transmission Assay, In Vitro

5) Product Images from "The Capsid-Associated UL25 Protein of the Alphaherpesvirus Pseudorabies Virus Is Nonessential for Cleavage and Encapsidation of Genomic DNA but Is Required for Nuclear Egress of Capsids"

Article Title: The Capsid-Associated UL25 Protein of the Alphaherpesvirus Pseudorabies Virus Is Nonessential for Cleavage and Encapsidation of Genomic DNA but Is Required for Nuclear Egress of Capsids

Journal: Journal of Virology

doi: 10.1128/JVI.02662-05

Immunolabeling of purified virions. Purified virion preparations and capsids in ultrathin sections were analyzed by immunoelectron microscopy after incubation with UL25 antiserum and gold-conjugated secondary antibodies. (A to C) Results from analysis of negatively stained purified virion preparations. (D to F) Intranuclear A, B, and C capsids in PrV-Ka-infected RK13 cells. (G to I) Different intranuclear capsid forms in PrV-ΔUL25F-infected cells serving as negative controls to demonstrate the specificity of the antiserum. Secondary antibodies tagged with 5-nm gold particles were used for panel C, whereas 10-nm gold-tagged antibodies were used for all other panels. Bars, 100 μm.
Figure Legend Snippet: Immunolabeling of purified virions. Purified virion preparations and capsids in ultrathin sections were analyzed by immunoelectron microscopy after incubation with UL25 antiserum and gold-conjugated secondary antibodies. (A to C) Results from analysis of negatively stained purified virion preparations. (D to F) Intranuclear A, B, and C capsids in PrV-Ka-infected RK13 cells. (G to I) Different intranuclear capsid forms in PrV-ΔUL25F-infected cells serving as negative controls to demonstrate the specificity of the antiserum. Secondary antibodies tagged with 5-nm gold particles were used for panel C, whereas 10-nm gold-tagged antibodies were used for all other panels. Bars, 100 μm.

Techniques Used: Immunolabeling, Purification, Immuno-Electron Microscopy, Incubation, Staining, Infection

6) Product Images from "The Pseudorabies Virus UL11 Protein Is a Virion Component Involved in Secondary Envelopment in the Cytoplasm"

Article Title: The Pseudorabies Virus UL11 Protein Is a Virion Component Involved in Secondary Envelopment in the Cytoplasm

Journal: Journal of Virology

doi: 10.1128/JVI.77.9.5339-5351.2003

Virion localization of the PrV UL11 protein. Purified extracellular PrV virions (A and B) as well as RK13 cells infected at an MOI of 1 for 12 h with PrV-Ka (C to F) were prepared for electron microscopy. Ultrathin sections (C to F) and purified virus particles (A and B) were subsequently incubated with anti-UL11 serum and 10-nm gold-tagged secondary anti-rabbit antibodies and counterstained. PrV UL11 was detected in partly disrupted, purified virus particles (A and B) as well as in enveloped intracytoplasmic (D and E) and extracellular virions (F) but was absent from primary enveloped virus particles in the perinuclear space (C). Bars, 100 nm in panels A to C and F and 200 nm in panels D and E.
Figure Legend Snippet: Virion localization of the PrV UL11 protein. Purified extracellular PrV virions (A and B) as well as RK13 cells infected at an MOI of 1 for 12 h with PrV-Ka (C to F) were prepared for electron microscopy. Ultrathin sections (C to F) and purified virus particles (A and B) were subsequently incubated with anti-UL11 serum and 10-nm gold-tagged secondary anti-rabbit antibodies and counterstained. PrV UL11 was detected in partly disrupted, purified virus particles (A and B) as well as in enveloped intracytoplasmic (D and E) and extracellular virions (F) but was absent from primary enveloped virus particles in the perinuclear space (C). Bars, 100 nm in panels A to C and F and 200 nm in panels D and E.

Techniques Used: Purification, Infection, Electron Microscopy, Incubation

7) Product Images from "Effects of Multiple Deletions of Murein Hydrolases on Viability, Septum Cleavage, and Sensitivity to Large Toxic Molecules in Escherichia coli"

Article Title: Effects of Multiple Deletions of Murein Hydrolases on Viability, Septum Cleavage, and Sensitivity to Large Toxic Molecules in Escherichia coli

Journal: Journal of Bacteriology

doi: 10.1128/JB.184.22.6093-6099.2002

Morphological changes of E. coli MHD64 treated with lysozyme. Lysozyme (10 μg/ml) was added to a growing culture (37°C) of E. coli MHD64 ( sltY amiABC mepA dacB pbpG ) in LB medium at an OD 578 of about 0.7. Samples ware taken 20 min later and prepared for electron microscopy as described in Materials and Methods. (a) Scanning electron microscopy. Bar = 5 μm. (b) Ultrathin section of a ball-like structure like those shown in panel a. Bar = 1 μm.
Figure Legend Snippet: Morphological changes of E. coli MHD64 treated with lysozyme. Lysozyme (10 μg/ml) was added to a growing culture (37°C) of E. coli MHD64 ( sltY amiABC mepA dacB pbpG ) in LB medium at an OD 578 of about 0.7. Samples ware taken 20 min later and prepared for electron microscopy as described in Materials and Methods. (a) Scanning electron microscopy. Bar = 5 μm. (b) Ultrathin section of a ball-like structure like those shown in panel a. Bar = 1 μm.

Techniques Used: Electron Microscopy

8) Product Images from "Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.) ▿ L.) ▿ †"

Article Title: Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.) ▿ L.) ▿ †

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.00133-10

Transmission electron micrographs of ultrathin sections of knots induced by P . savastanoi pv. savastanoi NCPPB 3335 (A and B) and T3 (C and D) at 35 days p.i. on olive plants. (A) Ultrastructure of the parenchymal knot tissue invaded by rod-shaped wild-type
Figure Legend Snippet: Transmission electron micrographs of ultrathin sections of knots induced by P . savastanoi pv. savastanoi NCPPB 3335 (A and B) and T3 (C and D) at 35 days p.i. on olive plants. (A) Ultrastructure of the parenchymal knot tissue invaded by rod-shaped wild-type

Techniques Used: Transmission Assay

9) Product Images from "Terracidiphilus gabretensis gen. nov., sp. nov., an Abundant and Active Forest Soil Acidobacterium Important in Organic Matter Transformation"

Article Title: Terracidiphilus gabretensis gen. nov., sp. nov., an Abundant and Active Forest Soil Acidobacterium Important in Organic Matter Transformation

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.03353-15

Electron TEM and SEM micrographs showing the morphology of T. gabretensis S55 T . (A to C) Images of ultrathin sections of strain S55 T . The capsule was well preserved using an alcian blue fixation protocol. The arrow in panel B points to the border of the
Figure Legend Snippet: Electron TEM and SEM micrographs showing the morphology of T. gabretensis S55 T . (A to C) Images of ultrathin sections of strain S55 T . The capsule was well preserved using an alcian blue fixation protocol. The arrow in panel B points to the border of the

Techniques Used: Transmission Electron Microscopy

10) Product Images from "Human Cytomegalovirus Infection of Caco-2 Cells Occurs at the Basolateral Membrane and Is Differentiation State Dependent"

Article Title: Human Cytomegalovirus Infection of Caco-2 Cells Occurs at the Basolateral Membrane and Is Differentiation State Dependent

Journal: Journal of Virology

doi:

Electron microscopy showing HCMV capsids in the nuclei of HCMV-infected Caco-2 cells. Caco-2 cell monolayers at a TER of 250 Ω · cm 2 were infected basolaterally at an MOI of 25. At day 20 p.i., monolayers were fixed, cut into ultrathin sections, and embedded before being viewed with a Philips EM 300 electron microscope.
Figure Legend Snippet: Electron microscopy showing HCMV capsids in the nuclei of HCMV-infected Caco-2 cells. Caco-2 cell monolayers at a TER of 250 Ω · cm 2 were infected basolaterally at an MOI of 25. At day 20 p.i., monolayers were fixed, cut into ultrathin sections, and embedded before being viewed with a Philips EM 300 electron microscope.

Techniques Used: Electron Microscopy, Infection, Microscopy

11) Product Images from "Isolation and Characterization of an Equine Foamy Virus"

Article Title: Isolation and Characterization of an Equine Foamy Virus

Journal: Journal of Virology

doi:

(A) Cytopathic effect in vitro on human U373-MG cells. Note the formation of syncytia (arrows) presenting numerous vacuoles. (B and C) Electron microscopy of ultrathin sections from infected cells. The viral particle has the typical FV appearance, enveloped particles surrounded by spikes and a clear central core.
Figure Legend Snippet: (A) Cytopathic effect in vitro on human U373-MG cells. Note the formation of syncytia (arrows) presenting numerous vacuoles. (B and C) Electron microscopy of ultrathin sections from infected cells. The viral particle has the typical FV appearance, enveloped particles surrounded by spikes and a clear central core.

Techniques Used: In Vitro, Electron Microscopy, Infection

12) Product Images from "Correlative Imaging of Fluorescent Proteins in Resin-Embedded Plant Material1"

Article Title: Correlative Imaging of Fluorescent Proteins in Resin-Embedded Plant Material1

Journal: Plant Physiology

doi: 10.1104/pp.112.212365

image of an ultrathin section of petiole from a plant expressing pSEO2.HDEL:GFP. The section was poststained with uranyl acetate and lead citrate. Scale = 5 μm. B, A semithin section acquired immediately after the
Figure Legend Snippet: image of an ultrathin section of petiole from a plant expressing pSEO2.HDEL:GFP. The section was poststained with uranyl acetate and lead citrate. Scale = 5 μm. B, A semithin section acquired immediately after the

Techniques Used: Expressing

13) Product Images from "The Omp85 protein of Neisseria meningitidis is required for lipid export to the outer membrane"

Article Title: The Omp85 protein of Neisseria meningitidis is required for lipid export to the outer membrane

Journal: The EMBO Journal

doi: 10.1093/emboj/cdg174

Fig. 3. Electron micrographs of ultrathin sections of H44/76 wild-type and CM omp85 grown in the presence and absence of 0.05 mM IPTG. ( A ) Overview and ( B – D ) high magnification of the cell envelope of the different strains. Bacteria were fixed in a Karnovsky solution and contrasted according to standard procedures (A and B), or they were fixed in a combined glutaraldehyde and OsO 4 solution to retain lipids and contrasted to standard procedures (C), and fixed in PFA, followed directly by embedding without contrasting (D1/2). Sections in (D1) were not post-stained and all the contrast was imparted by the embedding medium. A cytochemical reaction for the presence of PAS-positive material on ultrathin sections is illustrated in (D2). Depletion of Omp85 leads to accumulation of electron-dense amorphous material (small arrows in A, white asterisk in B) and to vesicular structures (large arrow in B) in the periplasmic space. This amorphous material is not lipophilic or cytochemically stained for polysaccharides (white asterisk in C and D2). The small arrows in (C) mark the osmiophilic granules outside the OM. The black asterisks in (D1) and (D2) denote a layer of PAS-positive material outside the cells, presumably the capsular polysaccharide. Arrowheads in (B–D) mark the peptidoglycan layer. The bars represent 200 µm in (A) and 100 µm in (B–D).
Figure Legend Snippet: Fig. 3. Electron micrographs of ultrathin sections of H44/76 wild-type and CM omp85 grown in the presence and absence of 0.05 mM IPTG. ( A ) Overview and ( B – D ) high magnification of the cell envelope of the different strains. Bacteria were fixed in a Karnovsky solution and contrasted according to standard procedures (A and B), or they were fixed in a combined glutaraldehyde and OsO 4 solution to retain lipids and contrasted to standard procedures (C), and fixed in PFA, followed directly by embedding without contrasting (D1/2). Sections in (D1) were not post-stained and all the contrast was imparted by the embedding medium. A cytochemical reaction for the presence of PAS-positive material on ultrathin sections is illustrated in (D2). Depletion of Omp85 leads to accumulation of electron-dense amorphous material (small arrows in A, white asterisk in B) and to vesicular structures (large arrow in B) in the periplasmic space. This amorphous material is not lipophilic or cytochemically stained for polysaccharides (white asterisk in C and D2). The small arrows in (C) mark the osmiophilic granules outside the OM. The black asterisks in (D1) and (D2) denote a layer of PAS-positive material outside the cells, presumably the capsular polysaccharide. Arrowheads in (B–D) mark the peptidoglycan layer. The bars represent 200 µm in (A) and 100 µm in (B–D).

Techniques Used: Staining

14) Product Images from "Disruption of Krox20–Nab Interaction in the Mouse Leads to Peripheral Neuropathy with Biphasic Evolution"

Article Title: Disruption of Krox20–Nab Interaction in the Mouse Leads to Peripheral Neuropathy with Biphasic Evolution

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.5187-07.2008

Krox20 I268F/I268F mice show a peripheral hypomyelination at P17. Macroscopic examination of brachial ( A , E , I ) and sciatic nerves ( B , F , J ) indicates that the homozygous mutant nerves are translucent and show an abnormal organization. Observations of toluidine blue-stained semithin sections ( C , G , K ) and electron microscopy analysis of ultrathin sections ( D , H , L ) of sciatic nerves show that the myelin from heterozygous mice is similar to the wild type, whereas it is altered in homozygous mutant animals. M–O , Higher magnifications confirm the presence of totally amyelinated fibers ( M , asterisks) and of myelinated fibers with an abnormally thin sheath and of unfoldings ( N , arrows) in the mutant mice. Macrophages ( O , arrowhead) are also observed. P , Distribution of P17 axons with a caliber superior to 1 μm according to their g -ratio (axon diameter/total myelinated fiber diameter). The statistical significance was analyzed using the Student's t test: * p
Figure Legend Snippet: Krox20 I268F/I268F mice show a peripheral hypomyelination at P17. Macroscopic examination of brachial ( A , E , I ) and sciatic nerves ( B , F , J ) indicates that the homozygous mutant nerves are translucent and show an abnormal organization. Observations of toluidine blue-stained semithin sections ( C , G , K ) and electron microscopy analysis of ultrathin sections ( D , H , L ) of sciatic nerves show that the myelin from heterozygous mice is similar to the wild type, whereas it is altered in homozygous mutant animals. M–O , Higher magnifications confirm the presence of totally amyelinated fibers ( M , asterisks) and of myelinated fibers with an abnormally thin sheath and of unfoldings ( N , arrows) in the mutant mice. Macrophages ( O , arrowhead) are also observed. P , Distribution of P17 axons with a caliber superior to 1 μm according to their g -ratio (axon diameter/total myelinated fiber diameter). The statistical significance was analyzed using the Student's t test: * p

Techniques Used: Mouse Assay, Mutagenesis, Staining, Electron Microscopy

15) Product Images from "Uroplakins play conserved roles in egg fertilization and acquired additional urothelial functions during mammalian divergence"

Article Title: Uroplakins play conserved roles in egg fertilization and acquired additional urothelial functions during mammalian divergence

Journal: Molecular Biology of the Cell

doi: 10.1091/mbc.E18-08-0496

Uroplakin association with cell-surface, multivesicular bodies, and exosomes of mouse oocytes. (A, B) Colocalization of uroplakins with (A) CD9 and (B) CD81 at egg plasma membranes (zona pellucida free). (C, D) Colocalization of UPII (S3045), CD9, CD81 by IF-staining of intact eggs with intact zona pellucida viewed at (C) low or (D) high magnification. Note that in A and B the precise and partial colocalization of UP with CD9 and CD81, respectively; in D the partial colocalization of UPII and CD9 on the exosomes in the PVS (yellow arrows) and the colocalization of UPII and CD81 on some exosomes in ZP (white arrows). Blue nuclear staining by Hoechst 33258 in C and D. (E–J) IEM localization by staining ultrathin sections of oocytes (E, G, H) or eggs (F, I, J) using antibodies to UPIa (E, G, I) and Ib (F, H) and ovastacin of cortical granules (J). Note the association of UP’s with oocyte surface (E), egg microvilli (F), multivesicular bodies (G, H1) and their intraluminal vesicles (H2; asterisks), and exosomes (I). Note the distinct staining patterns of the uroplakin-positive MVBs (G, H1) and cortical granules (J; asterisks). (K) Transmission electron microscopy of an egg from a UPII-knockout mouse showing unusual multilobular bodies (**) possibly representing autophagasomes. Uroplakin antibodies: (A, E, and I, Ia128; B, C, and D, II S3045; and F and H, Ib 7727). Bars equal to 2 µm (A, B, D, and K), 10 µm (C), 0.5 µm (E-G, H1, I and J), or 0.2 µm (H2).
Figure Legend Snippet: Uroplakin association with cell-surface, multivesicular bodies, and exosomes of mouse oocytes. (A, B) Colocalization of uroplakins with (A) CD9 and (B) CD81 at egg plasma membranes (zona pellucida free). (C, D) Colocalization of UPII (S3045), CD9, CD81 by IF-staining of intact eggs with intact zona pellucida viewed at (C) low or (D) high magnification. Note that in A and B the precise and partial colocalization of UP with CD9 and CD81, respectively; in D the partial colocalization of UPII and CD9 on the exosomes in the PVS (yellow arrows) and the colocalization of UPII and CD81 on some exosomes in ZP (white arrows). Blue nuclear staining by Hoechst 33258 in C and D. (E–J) IEM localization by staining ultrathin sections of oocytes (E, G, H) or eggs (F, I, J) using antibodies to UPIa (E, G, I) and Ib (F, H) and ovastacin of cortical granules (J). Note the association of UP’s with oocyte surface (E), egg microvilli (F), multivesicular bodies (G, H1) and their intraluminal vesicles (H2; asterisks), and exosomes (I). Note the distinct staining patterns of the uroplakin-positive MVBs (G, H1) and cortical granules (J; asterisks). (K) Transmission electron microscopy of an egg from a UPII-knockout mouse showing unusual multilobular bodies (**) possibly representing autophagasomes. Uroplakin antibodies: (A, E, and I, Ia128; B, C, and D, II S3045; and F and H, Ib 7727). Bars equal to 2 µm (A, B, D, and K), 10 µm (C), 0.5 µm (E-G, H1, I and J), or 0.2 µm (H2).

Techniques Used: Staining, Transmission Assay, Electron Microscopy, Knock-Out

16) Product Images from "Olfactory and solitary chemosensory cells: two different chemosensory systems in the nasal cavity of the American alligator, Alligator mississippiensis"

Article Title: Olfactory and solitary chemosensory cells: two different chemosensory systems in the nasal cavity of the American alligator, Alligator mississippiensis

Journal: BMC Neuroscience

doi: 10.1186/1471-2202-8-64

A – D Histological staining nuclear red/light green/orange G . Note that A – D are depicted at the same magnification and that all zones contain OSNs (based on results from semi- and ultrathin sections). A Olfactory epithelium in zone 1 close to the naris. B Ventrolateral olfactory epithelium in zone 2. The height of the epithelium is variable. Bowman glands (bg) are present. bv – blood vessel. C Olfactory epithelium in zone 4 (anterior turbinate). bv – blood vessel. D Olfactory epithelium in zone 4 (anterior turbinate) in a different region than shown in C. bg – Bowman gland. E – F Semithin (1 μm) sections stained with toluidin blue. Both E and F depict zone 3. Bowman glands are numerous (bg). Note in F how few OSNs are present (arrows). Sc shows a nucleus in the layer of nuclei of the supporting cells. osn shows the layer of nuclei of the olfactory neurons and bc the layer of basal cells. bg – Bowman gland, bv – blood vessel, ms – muscle.
Figure Legend Snippet: A – D Histological staining nuclear red/light green/orange G . Note that A – D are depicted at the same magnification and that all zones contain OSNs (based on results from semi- and ultrathin sections). A Olfactory epithelium in zone 1 close to the naris. B Ventrolateral olfactory epithelium in zone 2. The height of the epithelium is variable. Bowman glands (bg) are present. bv – blood vessel. C Olfactory epithelium in zone 4 (anterior turbinate). bv – blood vessel. D Olfactory epithelium in zone 4 (anterior turbinate) in a different region than shown in C. bg – Bowman gland. E – F Semithin (1 μm) sections stained with toluidin blue. Both E and F depict zone 3. Bowman glands are numerous (bg). Note in F how few OSNs are present (arrows). Sc shows a nucleus in the layer of nuclei of the supporting cells. osn shows the layer of nuclei of the olfactory neurons and bc the layer of basal cells. bg – Bowman gland, bv – blood vessel, ms – muscle.

Techniques Used: Staining, Mass Spectrometry

17) Product Images from "Theta-Burst Transcranial Magnetic Stimulation Alters Cortical Inhibition"

Article Title: Theta-Burst Transcranial Magnetic Stimulation Alters Cortical Inhibition

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.1379-10.2011

Immunelectron microscopic analysis of PV-positive interneurons and WFA-stained perineuronal nets. PV ( a 1–5 ) and perineuronal net (WFA) ( b 1,2 ) immunoperoxidase labeling of the motor cortex in iTBS-treated rat brain (acute, 2 h). Toluidine blue-stained 0.8-μm-thick semithin section of an area with reduced PV expression shows no signs of cell death ( a 1,2 ). Adjacent semithin section ( a 3 ) displays single PV-positive neurons with faint (arrows) and strong (arrowheads) immunoreactivity. Electron microscopy exhibits PV-positive interneurons, both with strong ( a 4 ) and weak ( a 5 ) PV immunoreactivity. Integrity of such interneurons is further confirmed by frequently surrounding WFA-positive perineuronal nets detected in toluidine blue-counterstained semithin ( b 1 ) and corresponding ultrathin sections ( b 2 ).
Figure Legend Snippet: Immunelectron microscopic analysis of PV-positive interneurons and WFA-stained perineuronal nets. PV ( a 1–5 ) and perineuronal net (WFA) ( b 1,2 ) immunoperoxidase labeling of the motor cortex in iTBS-treated rat brain (acute, 2 h). Toluidine blue-stained 0.8-μm-thick semithin section of an area with reduced PV expression shows no signs of cell death ( a 1,2 ). Adjacent semithin section ( a 3 ) displays single PV-positive neurons with faint (arrows) and strong (arrowheads) immunoreactivity. Electron microscopy exhibits PV-positive interneurons, both with strong ( a 4 ) and weak ( a 5 ) PV immunoreactivity. Integrity of such interneurons is further confirmed by frequently surrounding WFA-positive perineuronal nets detected in toluidine blue-counterstained semithin ( b 1 ) and corresponding ultrathin sections ( b 2 ).

Techniques Used: Staining, Labeling, Expressing, Electron Microscopy

18) Product Images from "Decay-Accelerating Factor and Cytoskeleton Redistribution Pattern in HeLa Cells Infected with Recombinant Escherichia coli Strains Expressing Dr Family of Adhesins"

Article Title: Decay-Accelerating Factor and Cytoskeleton Redistribution Pattern in HeLa Cells Infected with Recombinant Escherichia coli Strains Expressing Dr Family of Adhesins

Journal: Infection and Immunity

doi:

TEM. (A) Initial steps of internalization. Four beads coated with purified Dr fimbriae are wrapped with HeLa cell microvilli (arrow). Microfilament accumulation can be seen at the sites of internalization (arrowheads). (B) Three internalized Dr fimbria-coated beads are located in the cytoplasm within tight membrane-bound vacuoles (arrows). (C) Control, BSA-coated polystyrene were not found in ultrathin sections of HeLa cells. The microvilli on most of the cells were less strongly expressed and located closer to the cell surface. Bars, 1 μm.
Figure Legend Snippet: TEM. (A) Initial steps of internalization. Four beads coated with purified Dr fimbriae are wrapped with HeLa cell microvilli (arrow). Microfilament accumulation can be seen at the sites of internalization (arrowheads). (B) Three internalized Dr fimbria-coated beads are located in the cytoplasm within tight membrane-bound vacuoles (arrows). (C) Control, BSA-coated polystyrene were not found in ultrathin sections of HeLa cells. The microvilli on most of the cells were less strongly expressed and located closer to the cell surface. Bars, 1 μm.

Techniques Used: Transmission Electron Microscopy, Purification

19) Product Images from "Binding of Human Plasminogen to Bifidobacterium ▿"

Article Title: Binding of Human Plasminogen to Bifidobacterium ▿

Journal: Journal of Bacteriology

doi: 10.1128/JB.00159-07

Visualization of human Plg on the cell surface of B. lactis BI07 by immunoelectron microscopy. Plg was detected on the bacterial cell surface by using anti-Plg antibody and a colloidal gold-coated secondary antibody in preembedding labeling experiments. Plg aggregates associated with the cell surface were visualized as black dots in ultrathin sections of bacterial cells at a magnification of ×13,000. Incubation of B. lactis BI07 cells with anti-Plg antibody resulted in no labeling (inset). In each experimental condition, for eight fields examined per sample, 70% of B. lactis BI07 cells were labeled with gold particles, whereas none of the negative control cells was labeled.
Figure Legend Snippet: Visualization of human Plg on the cell surface of B. lactis BI07 by immunoelectron microscopy. Plg was detected on the bacterial cell surface by using anti-Plg antibody and a colloidal gold-coated secondary antibody in preembedding labeling experiments. Plg aggregates associated with the cell surface were visualized as black dots in ultrathin sections of bacterial cells at a magnification of ×13,000. Incubation of B. lactis BI07 cells with anti-Plg antibody resulted in no labeling (inset). In each experimental condition, for eight fields examined per sample, 70% of B. lactis BI07 cells were labeled with gold particles, whereas none of the negative control cells was labeled.

Techniques Used: Immuno-Electron Microscopy, Labeling, Incubation, Negative Control

20) Product Images from "The Glutamate Transporter EAAT4 in Rat Cerebellar Purkinje Cells: A Glutamate-Gated Chloride Channel Concentrated near the Synapse in Parts of the Dendritic Membrane Facing Astroglia"

Article Title: The Glutamate Transporter EAAT4 in Rat Cerebellar Purkinje Cells: A Glutamate-Gated Chloride Channel Concentrated near the Synapse in Parts of the Dendritic Membrane Facing Astroglia

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.18-10-03606.1998

Pre-embedding electron microscopic immunocytochemistry (peroxidase) demonstrates EAAT4 in spinae ( S ) of the Purkinje cells in the cerebellar molecular layer. The nerve terminals ( T ) are not stained even when opened by the vibratome knife ( asterisk ). The staining intensity is strongest near the cutting surface and decreases gradually inward into the section. (A spine close to the scale bar appears negative, probably because of lack of penetration of the immunoreagents.) The fixed tissue was incubated with 3 μg/ml rabbit anti-D537 antibodies and processed for immunoperoxidase, before embedding and cutting of ultrathin sections. Scale bar, 0.26 μm. A , Astroglial process.
Figure Legend Snippet: Pre-embedding electron microscopic immunocytochemistry (peroxidase) demonstrates EAAT4 in spinae ( S ) of the Purkinje cells in the cerebellar molecular layer. The nerve terminals ( T ) are not stained even when opened by the vibratome knife ( asterisk ). The staining intensity is strongest near the cutting surface and decreases gradually inward into the section. (A spine close to the scale bar appears negative, probably because of lack of penetration of the immunoreagents.) The fixed tissue was incubated with 3 μg/ml rabbit anti-D537 antibodies and processed for immunoperoxidase, before embedding and cutting of ultrathin sections. Scale bar, 0.26 μm. A , Astroglial process.

Techniques Used: Immunocytochemistry, Staining, Incubation

21) Product Images from "Beta-adrenergic receptors: astrocytic localization in the adult visual cortex and their relation to catecholamine axon terminals as revealed by electron microscopic immunocytochemistry"

Article Title: Beta-adrenergic receptors: astrocytic localization in the adult visual cortex and their relation to catecholamine axon terminals as revealed by electron microscopic immunocytochemistry

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

doi: 10.1523/JNEUROSCI.12-03-00781.1992

Electron micrographs obtained from semiadjacent ultrathin sections showing the relation between ( β AR404-immunoreactive astrocytic processes and a catecholamine terminal containing few vesicles. The gold-labeled catecholamine terminal ( CT ) forms a small axospinous symmetric contact with a dendritic spine ( solid arrow in B points to the postsynaptic membrane). This junction is not evident in A . The same spine receives synaptic input from an unlabeled terminal ( uT ), Open arrows in A and B point to the thick postsynaptic densities that are associated with unlabeled terminals. There are several unlabeled processes between the β AR404-immunoreactive astrocytic processes ( β-A ) and catecholamine terminals ( CT ). Scale bar, 0.5 μ m.
Figure Legend Snippet: Electron micrographs obtained from semiadjacent ultrathin sections showing the relation between ( β AR404-immunoreactive astrocytic processes and a catecholamine terminal containing few vesicles. The gold-labeled catecholamine terminal ( CT ) forms a small axospinous symmetric contact with a dendritic spine ( solid arrow in B points to the postsynaptic membrane). This junction is not evident in A . The same spine receives synaptic input from an unlabeled terminal ( uT ), Open arrows in A and B point to the thick postsynaptic densities that are associated with unlabeled terminals. There are several unlabeled processes between the β AR404-immunoreactive astrocytic processes ( β-A ) and catecholamine terminals ( CT ). Scale bar, 0.5 μ m.

Techniques Used: Labeling

Electron micrographs showing β AR404-immunoreactive astrocytic processes that encircle one catecholamine varicosity and two unlabeled synaptic terminals. These electron micrographs were obtained from serially collected ultrathin sections of the rat visual cortex. In A, two of the β AR404-immunoreactive processes ( β-A ) form gap junctions with each other ( arrowhead pair ). In B, portions of the lower right astrocytic process ( asterisks in the cytoplasm) course next to the terminal identifiable as catecholamine-containing by the presence of silver-intensified gold particles reflecting immunoreactivity to TH. Catecholamine-containing terminal ( CT ) forms a contact with a small dendritic spine ( solid arrow in B ). Three unlabeled terminals ( uT1, uT2, and uT3 ) also are juxtaposed to the β AR404-immunoreactive astrocytic process. Open arrows in B point to postsynaptic densities within dendritic spines that are associated with uT2 and uT3. Scale bar, 0.5 μ m.
Figure Legend Snippet: Electron micrographs showing β AR404-immunoreactive astrocytic processes that encircle one catecholamine varicosity and two unlabeled synaptic terminals. These electron micrographs were obtained from serially collected ultrathin sections of the rat visual cortex. In A, two of the β AR404-immunoreactive processes ( β-A ) form gap junctions with each other ( arrowhead pair ). In B, portions of the lower right astrocytic process ( asterisks in the cytoplasm) course next to the terminal identifiable as catecholamine-containing by the presence of silver-intensified gold particles reflecting immunoreactivity to TH. Catecholamine-containing terminal ( CT ) forms a contact with a small dendritic spine ( solid arrow in B ). Three unlabeled terminals ( uT1, uT2, and uT3 ) also are juxtaposed to the β AR404-immunoreactive astrocytic process. Open arrows in B point to postsynaptic densities within dendritic spines that are associated with uT2 and uT3. Scale bar, 0.5 μ m.

Techniques Used:

22) Product Images from "Targeted Disruption of Core 1 ?1,3-galactosyltransferase (C1galt1) Induces Apical Endocytic Trafficking in Human Corneal Keratinocytes"

Article Title: Targeted Disruption of Core 1 ?1,3-galactosyltransferase (C1galt1) Induces Apical Endocytic Trafficking in Human Corneal Keratinocytes

Journal: PLoS ONE

doi: 10.1371/journal.pone.0036628

C1galt1-deficient human corneal keratinocytes display plasma membrane invaginations on the apical surface. (A) Scheme for mucin-type O-glycan biosynthesis. The core 1 β,3-galactosyltransferase (C1galt1 or T-synthase) is a key branchpoint enzyme that directs the synthesis of core 1 (T-antigen), the precursor structure for many extended mucin-type O-glycans in a wide variety of glycoproteins. (B) Electron micrograph at low magnification of an ultrathin section (60 to 90-Å) of C1galt1 keratinocytes grown on Transwell® inserts demonstrating cell stratification and the presence of flattened epithelial cells on the apical layer (top) Scale bar, 10 µm. Membrane invaginations (arrowheads, inset) can be observed occasionally on the apical portion of the plasma membrane in apical cells of C1galt1 keratinocytes (middle), more frequently than in scramble control (bottom). Scale bar, 200 nm.
Figure Legend Snippet: C1galt1-deficient human corneal keratinocytes display plasma membrane invaginations on the apical surface. (A) Scheme for mucin-type O-glycan biosynthesis. The core 1 β,3-galactosyltransferase (C1galt1 or T-synthase) is a key branchpoint enzyme that directs the synthesis of core 1 (T-antigen), the precursor structure for many extended mucin-type O-glycans in a wide variety of glycoproteins. (B) Electron micrograph at low magnification of an ultrathin section (60 to 90-Å) of C1galt1 keratinocytes grown on Transwell® inserts demonstrating cell stratification and the presence of flattened epithelial cells on the apical layer (top) Scale bar, 10 µm. Membrane invaginations (arrowheads, inset) can be observed occasionally on the apical portion of the plasma membrane in apical cells of C1galt1 keratinocytes (middle), more frequently than in scramble control (bottom). Scale bar, 200 nm.

Techniques Used:

23) Product Images from "Downregulation of the Petunia hybrida ?-Expansin Gene PhEXP1 Reduces the Amount of Crystalline Cellulose in Cell Walls and Leads to Phenotypic Changes in Petal Limbs"

Article Title: Downregulation of the Petunia hybrida ?-Expansin Gene PhEXP1 Reduces the Amount of Crystalline Cellulose in Cell Walls and Leads to Phenotypic Changes in Petal Limbs

Journal: The Plant Cell

doi: 10.1105/tpc.018705

Changes in Cell Shape in Line 25. (A) and (B) Scanning electron micrographs of abaxial epidermal limb cells at anthesis. Bars = 50 μm. (C) and (D) Tangential semithin sections of abaxial epidermis of petal limb at anthesis. Arrows indicate the characteristic cell wall lobes. Bars = 25 μm. (E) and (F) Transmission electron micrographs of tangential ultrathin sections of the abaxial epidermis of petal limb at anthesis. cw, cell wall; n, nucleus. Bars = 5 μm.
Figure Legend Snippet: Changes in Cell Shape in Line 25. (A) and (B) Scanning electron micrographs of abaxial epidermal limb cells at anthesis. Bars = 50 μm. (C) and (D) Tangential semithin sections of abaxial epidermis of petal limb at anthesis. Arrows indicate the characteristic cell wall lobes. Bars = 25 μm. (E) and (F) Transmission electron micrographs of tangential ultrathin sections of the abaxial epidermis of petal limb at anthesis. cw, cell wall; n, nucleus. Bars = 5 μm.

Techniques Used: Transmission Assay

24) Product Images from "The cysteine proteinase inhibitor Z-Phe-Ala-CHN2 alters cell morphology and cell division activity of Trypanosoma brucei bloodstream forms in vivo"

Article Title: The cysteine proteinase inhibitor Z-Phe-Ala-CHN2 alters cell morphology and cell division activity of Trypanosoma brucei bloodstream forms in vivo

Journal: Kinetoplastid Biology and Disease

doi: 10.1186/1475-9292-6-2

Effect of Z-Phe-Ala-CHN 2 on the size of the lysosome of T. brucei bloodstream forms in vivo . Mice were infected and treated as described in the legend to Fig. 1. On day 5 p.i., trypanosomes were purified and processed for electron microscopy. Ultrathin sections of representative cells purified from mice treated with Z-Phe-Ala-CHN 2 ( a ) and vehicle alone ( b ) are shown. Note the enlarged lysosome in the trypanosome exposed to Z-Phe-Ala-CHN 2 compared with that in the short-stumpy form from control mice. fl, flagellum; fp, flagellar pocket; ly, lysosome, m, mitochondrion. Bar, 0.5 μm.
Figure Legend Snippet: Effect of Z-Phe-Ala-CHN 2 on the size of the lysosome of T. brucei bloodstream forms in vivo . Mice were infected and treated as described in the legend to Fig. 1. On day 5 p.i., trypanosomes were purified and processed for electron microscopy. Ultrathin sections of representative cells purified from mice treated with Z-Phe-Ala-CHN 2 ( a ) and vehicle alone ( b ) are shown. Note the enlarged lysosome in the trypanosome exposed to Z-Phe-Ala-CHN 2 compared with that in the short-stumpy form from control mice. fl, flagellum; fp, flagellar pocket; ly, lysosome, m, mitochondrion. Bar, 0.5 μm.

Techniques Used: In Vivo, Mouse Assay, Infection, Purification, Electron Microscopy

25) Product Images from "Vesicle-Mediated Transport and Release of CCL21 in Endangered Neurons: A Possible Explanation for Microglia Activation Remote from a Primary Lesion"

Article Title: Vesicle-Mediated Transport and Release of CCL21 in Endangered Neurons: A Possible Explanation for Microglia Activation Remote from a Primary Lesion

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.1019-05.2005

GSSP intensification and analysis of ultrathin sections of cultured neurons by electron microscopy showed the presence of CCL21-positive vesicles in the Golgi or TGN near the axonal hillock (arrowheads, A ), in presynaptic structures (arrowheads, B ), and in the synaptic cleft (arrowheads, C ). These microphotographs were taken from untreated neurons because treatment with glutamate had a major impact on the ultrastructural architecture of the cells. However, no major differences were found between the CCL21 staining patterns in treated versus untreated neurons (data not shown). Scale bars: A , 500 nm; B , 200 nm; C , 100 nm. Similar results have been obtained in two independent experiments.
Figure Legend Snippet: GSSP intensification and analysis of ultrathin sections of cultured neurons by electron microscopy showed the presence of CCL21-positive vesicles in the Golgi or TGN near the axonal hillock (arrowheads, A ), in presynaptic structures (arrowheads, B ), and in the synaptic cleft (arrowheads, C ). These microphotographs were taken from untreated neurons because treatment with glutamate had a major impact on the ultrastructural architecture of the cells. However, no major differences were found between the CCL21 staining patterns in treated versus untreated neurons (data not shown). Scale bars: A , 500 nm; B , 200 nm; C , 100 nm. Similar results have been obtained in two independent experiments.

Techniques Used: Cell Culture, Electron Microscopy, Staining

26) Product Images from "Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus"

Article Title: Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.23-35-11026.2003

Electron micrographs showing immunoreactivity for GABA B1a/b and GABA B2 subunits in the stratum lucidum of CA3 and in the dentate molecular layer. A , In the stratum lucidum of CA3, peroxidase reaction end product for GABA B1a/b protein was observed in dendritic spines (s) of pyramidal cells postsynaptic to mossy fiber terminals (MT). B, C , Serial ultrathin sections showing immunogold particles for GABA B1a/b at the extrasynaptic membrane of spines (s 1 -s 3 , arrows) and over the presynaptic membrane specialization of a mossy fiber terminal (MT, double arrows). D , Immunostaining for GABA B2 was detected in spines (s) and a dendritic shaft of a presumed pyramidal cell (Den). E , Immunoparticles for GABA B2 were localized to the extrasynaptic plasma membrane of spines (s) and dendritic shafts (Den, arrows) and occasionally to the presynaptic membrane specialization of mossy fiber terminals (MT, double arrows). F, G , In the dentate molecular layer, peroxidase staining for the GABA B1a/b subunit was weak in spines (s) and in a dendritic shaft of a presumed granule cell (Den). H , Immunogold particles for GABA B1a/b were located on the extrasynaptic membrane of spines (s) and dendritic shafts (Den) (arrows). I , Immunostaining for GABA B2 was observed on spines (s) and dendritic shafts (Den) of presumed granule cells. J , Immunoparticles for GABA B2 were localized to extrasynaptic membranes (arrows). Scale bars, 0.2 μm.
Figure Legend Snippet: Electron micrographs showing immunoreactivity for GABA B1a/b and GABA B2 subunits in the stratum lucidum of CA3 and in the dentate molecular layer. A , In the stratum lucidum of CA3, peroxidase reaction end product for GABA B1a/b protein was observed in dendritic spines (s) of pyramidal cells postsynaptic to mossy fiber terminals (MT). B, C , Serial ultrathin sections showing immunogold particles for GABA B1a/b at the extrasynaptic membrane of spines (s 1 -s 3 , arrows) and over the presynaptic membrane specialization of a mossy fiber terminal (MT, double arrows). D , Immunostaining for GABA B2 was detected in spines (s) and a dendritic shaft of a presumed pyramidal cell (Den). E , Immunoparticles for GABA B2 were localized to the extrasynaptic plasma membrane of spines (s) and dendritic shafts (Den, arrows) and occasionally to the presynaptic membrane specialization of mossy fiber terminals (MT, double arrows). F, G , In the dentate molecular layer, peroxidase staining for the GABA B1a/b subunit was weak in spines (s) and in a dendritic shaft of a presumed granule cell (Den). H , Immunogold particles for GABA B1a/b were located on the extrasynaptic membrane of spines (s) and dendritic shafts (Den) (arrows). I , Immunostaining for GABA B2 was observed on spines (s) and dendritic shafts (Den) of presumed granule cells. J , Immunoparticles for GABA B2 were localized to extrasynaptic membranes (arrows). Scale bars, 0.2 μm.

Techniques Used: Immunostaining, Staining

27) Product Images from "Intracellular Interaction of Simian Immunodeficiency Virus Gag and Env Proteins"

Article Title: Intracellular Interaction of Simian Immunodeficiency Virus Gag and Env Proteins

Journal: Journal of Virology

doi:

Analysis of the site of SIV assembly by electron microscopy. HeLa T4 cells were transfected, fixed, and embedded, and ultrathin sections were stained with tannic acid and examined by electron microscopy. VLPs budding from cells coexpressing wt Env and Gag proteins (A), Gag and Env164RS (B), Gag and Env37RS (C), and Gag and Env18RS (D). Magnification, ×80,000.
Figure Legend Snippet: Analysis of the site of SIV assembly by electron microscopy. HeLa T4 cells were transfected, fixed, and embedded, and ultrathin sections were stained with tannic acid and examined by electron microscopy. VLPs budding from cells coexpressing wt Env and Gag proteins (A), Gag and Env164RS (B), Gag and Env37RS (C), and Gag and Env18RS (D). Magnification, ×80,000.

Techniques Used: Electron Microscopy, Transfection, Staining

28) Product Images from "Granule Cell Ascending Axon Excitatory Synapses onto Golgi Cells Implement a Potent Feedback Circuit in the Cerebellar Granular Layer"

Article Title: Granule Cell Ascending Axon Excitatory Synapses onto Golgi Cells Implement a Potent Feedback Circuit in the Cerebellar Granular Layer

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.4897-11.2013

Excitatory contacts onto Golgi cells in the GL identified with electron microscopy. Analysis of ultrathin cuts of GlyT2-GFP mice cerebella stained with an anti-GFP antibody amplified with the peroxidasic system to identify Golgi cells. A , A peroxidase-stained GoC dendrite (boxed regions B and C) penetrates a glomerulus (glom), well differentiated from surrounding GrC somata (gr), in the proximity of a GoC soma (Go). B , C , Intraglomerular articulations formed by the dendrites boxed in A shown at higher magnification. GoC dendrites (Go) receive asymmetric synapses from a mossy fiber terminal (MF) at both locations. There are numerous and densely packed vesicles in mossy fiber terminals. Note the postsynaptic densities on Golgi cell dendrites (white arrowheads). D1 , Low-magnification view of a stained GoC dendrite running in the GL in between GrC bodies and outside of a nearby glomerulus. D2 , Enlargement of the region boxed in D1, showing that the GoC dendrite is separated from the glomerulus by two fibers, running parallel to each other. One of these fibers (black arrowheads), putatively a GrC ascending axon, forms an asymmetric synaptic contact with the GoC dendrite (white arrowhead). E1 , Low-magnification view of a GoC body surrounded by granule cell bodies (gr) in the GL. E2 , Serial sections were acquired, and a low resolution image of another section is shown. E3 , Magnification of the boxed region in E2 showing an aa bundle (“beam”) running outside of glomeruli and forming asymmetric synapses with the GoC body. Black arrowheads indicate an individual fiber; white arrowhead indicates the corresponding postsynaptic density.
Figure Legend Snippet: Excitatory contacts onto Golgi cells in the GL identified with electron microscopy. Analysis of ultrathin cuts of GlyT2-GFP mice cerebella stained with an anti-GFP antibody amplified with the peroxidasic system to identify Golgi cells. A , A peroxidase-stained GoC dendrite (boxed regions B and C) penetrates a glomerulus (glom), well differentiated from surrounding GrC somata (gr), in the proximity of a GoC soma (Go). B , C , Intraglomerular articulations formed by the dendrites boxed in A shown at higher magnification. GoC dendrites (Go) receive asymmetric synapses from a mossy fiber terminal (MF) at both locations. There are numerous and densely packed vesicles in mossy fiber terminals. Note the postsynaptic densities on Golgi cell dendrites (white arrowheads). D1 , Low-magnification view of a stained GoC dendrite running in the GL in between GrC bodies and outside of a nearby glomerulus. D2 , Enlargement of the region boxed in D1, showing that the GoC dendrite is separated from the glomerulus by two fibers, running parallel to each other. One of these fibers (black arrowheads), putatively a GrC ascending axon, forms an asymmetric synaptic contact with the GoC dendrite (white arrowhead). E1 , Low-magnification view of a GoC body surrounded by granule cell bodies (gr) in the GL. E2 , Serial sections were acquired, and a low resolution image of another section is shown. E3 , Magnification of the boxed region in E2 showing an aa bundle (“beam”) running outside of glomeruli and forming asymmetric synapses with the GoC body. Black arrowheads indicate an individual fiber; white arrowhead indicates the corresponding postsynaptic density.

Techniques Used: Electron Microscopy, Mouse Assay, Staining, Amplification

29) Product Images from "Neurodegeneration in the olfactory bulb and olfactory deficits in the Ccdc66 -/- mouse model for retinal degeneration"

Article Title: Neurodegeneration in the olfactory bulb and olfactory deficits in the Ccdc66 -/- mouse model for retinal degeneration

Journal: IBRO Reports

doi: 10.1016/j.ibror.2018.08.004

Electron microscopy of the olfactory bulb glomeruli in Ccdc66 -/- and Ccdc66 +/+ mice. A) Ultrathin section of 3 month-old Ccdc66 -/- glomeruli revealed single rounded structures (x) with accumulations of membrane-surrounded autophagic-like vesicles (black arrowheads in the enlarged inlay in A ), some of which filled with electron-dense deposits corresponding to autophagosomes, not observed in age-matched Ccdc66 +/+ mice (B) . C) At 12 months of age, multiple structures with these autophagic-like vesicles are mainly localized in the dark appearing unmyelinated glomerular olfactory nerve fibers (ONF), not detected in age-matched Ccdc66 +/+ ( D ). E ) Enlargement of C showed many vesicles surrounded by membranes (arrowheads) within the dark axoplasm of the ONF. F) Autophagic-like vesicles were also detected in synaptic terminals (T) contacting a dendrite (De). Two postsynaptic densities are marked by arrows. AG, astroglia. M, mitochrondion. Scale bar in A, B, C, D : 1 μm, in E, F : 0.2 μm.
Figure Legend Snippet: Electron microscopy of the olfactory bulb glomeruli in Ccdc66 -/- and Ccdc66 +/+ mice. A) Ultrathin section of 3 month-old Ccdc66 -/- glomeruli revealed single rounded structures (x) with accumulations of membrane-surrounded autophagic-like vesicles (black arrowheads in the enlarged inlay in A ), some of which filled with electron-dense deposits corresponding to autophagosomes, not observed in age-matched Ccdc66 +/+ mice (B) . C) At 12 months of age, multiple structures with these autophagic-like vesicles are mainly localized in the dark appearing unmyelinated glomerular olfactory nerve fibers (ONF), not detected in age-matched Ccdc66 +/+ ( D ). E ) Enlargement of C showed many vesicles surrounded by membranes (arrowheads) within the dark axoplasm of the ONF. F) Autophagic-like vesicles were also detected in synaptic terminals (T) contacting a dendrite (De). Two postsynaptic densities are marked by arrows. AG, astroglia. M, mitochrondion. Scale bar in A, B, C, D : 1 μm, in E, F : 0.2 μm.

Techniques Used: Electron Microscopy, Mouse Assay

30) Product Images from "Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons"

Article Title: Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons

Journal: Neuron

doi: 10.1016/j.neuron.2017.01.019

Ultrastructural analysis of the accumulations of markers of DAergic axons in SJ1 RQ -KI dorsal striata (A) Anti-TH immunogold (15nm gold particles) labeling of ultrathin frozen sections of WT and SJ1 RQ -KI dorsal striata. Immunogold particles were detected in a subset of neuronal processes in the striata of both genotypes, indicating specific labeling for DAergic axons. (B) Immunogold labeling of ultrathin frozen sections of SJ1 RQ -KI dorsal striata with anti-TH or control (anti-GFP) rabbit antibodies. Gold labeling of an onion-like multilayered membrane structure is observed with the anti-TH antibodies (right), while a multilayered membrane structure indicated by white arrows (left) is not labeled by control antibodies. (C) Two examples of multilayered membrane structures visualized by conventional EM in the dorsal striatum of SJ1 RQ -KI mice. CB = cell bodies of medium spiny neurons. (D) Multilayered membrane structures (outlined in yellow) present in a 100×100×5μm volume of WT and SJ1 RQ -KI dorsal striata as assessed by SBEM analysis. 3D rendering of the outer surface of these structures is shown against a micrograph of the last image of the SBEM series. (E) Large clusters of DAT immunoreactivity present in a 100×100×5μm volume of WT and SJ1 RQ -KI dorsal striata as visualized by 3D reconstruction of confocal Z-stack images. Note the similar overall abundance of large DAT-positive structures (E) and multilayered membrane structures observed in D. for additional data.
Figure Legend Snippet: Ultrastructural analysis of the accumulations of markers of DAergic axons in SJ1 RQ -KI dorsal striata (A) Anti-TH immunogold (15nm gold particles) labeling of ultrathin frozen sections of WT and SJ1 RQ -KI dorsal striata. Immunogold particles were detected in a subset of neuronal processes in the striata of both genotypes, indicating specific labeling for DAergic axons. (B) Immunogold labeling of ultrathin frozen sections of SJ1 RQ -KI dorsal striata with anti-TH or control (anti-GFP) rabbit antibodies. Gold labeling of an onion-like multilayered membrane structure is observed with the anti-TH antibodies (right), while a multilayered membrane structure indicated by white arrows (left) is not labeled by control antibodies. (C) Two examples of multilayered membrane structures visualized by conventional EM in the dorsal striatum of SJ1 RQ -KI mice. CB = cell bodies of medium spiny neurons. (D) Multilayered membrane structures (outlined in yellow) present in a 100×100×5μm volume of WT and SJ1 RQ -KI dorsal striata as assessed by SBEM analysis. 3D rendering of the outer surface of these structures is shown against a micrograph of the last image of the SBEM series. (E) Large clusters of DAT immunoreactivity present in a 100×100×5μm volume of WT and SJ1 RQ -KI dorsal striata as visualized by 3D reconstruction of confocal Z-stack images. Note the similar overall abundance of large DAT-positive structures (E) and multilayered membrane structures observed in D. for additional data.

Techniques Used: Labeling, Mouse Assay

31) Product Images from "Tocopherol Derivative TFA-12 Promotes Myelin Repair in Experimental Models of Multiple Sclerosis"

Article Title: Tocopherol Derivative TFA-12 Promotes Myelin Repair in Experimental Models of Multiple Sclerosis

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.0774-13.2013

TFA-12 treatment enhances amount of remyelinated axons in LPC-induced lesions. A , Schematic representation of LPC-induced demyelination in the mouse spinal cord dorsal funiculus. B , Toluidine blue semithin section illustrating LPC-induced demyelination at 2 dpi. C , D , Ultrathin micrographs of the lesions in vehicle-treated ( C ) and TFA-12-treated ( D ) mice at 15 dpi. TFA-12 treatment enhances the number of remyelinated axons, identified by their thin myelin sheaths ( D , asterisks), whereas vehicle-treated mice had significantly fewer remyelinated axons ( C ). E , Quantification of remyelinated axons over the total number of axons within the lesion shows a significant increase under TFA-12 treatment. *** p
Figure Legend Snippet: TFA-12 treatment enhances amount of remyelinated axons in LPC-induced lesions. A , Schematic representation of LPC-induced demyelination in the mouse spinal cord dorsal funiculus. B , Toluidine blue semithin section illustrating LPC-induced demyelination at 2 dpi. C , D , Ultrathin micrographs of the lesions in vehicle-treated ( C ) and TFA-12-treated ( D ) mice at 15 dpi. TFA-12 treatment enhances the number of remyelinated axons, identified by their thin myelin sheaths ( D , asterisks), whereas vehicle-treated mice had significantly fewer remyelinated axons ( C ). E , Quantification of remyelinated axons over the total number of axons within the lesion shows a significant increase under TFA-12 treatment. *** p

Techniques Used: Mouse Assay

32) Product Images from "Characterization of human breast cancer epithelial cells (HBCEC) derived from long term cultured biopsies"

Article Title: Characterization of human breast cancer epithelial cells (HBCEC) derived from long term cultured biopsies

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/1756-9966-28-127

Characterization of primary human breast cancer epithelial cells (HBCEC) . A . Scanning electron micrographs of human breast cancer-derived cell cultures. The cells are squamous with many short and thin processes and grow upon each other. B . Ultrathin sections of two human breast cancer-derived cells, which partially overlap and are connected by desmosomes. The cells contain bundles of intermediate filaments and cytoplasmic vacuoles, whereas organelles are almost absent. In the right transmission micrograph, two squamous cell processes are connected by desmosomes and bundles of intermediate filaments are orientated in parallel to the cell surface. C . Immunofluorescence of intermediate filaments. Nuclei became visual using DAPI and the intermediate filament proteins cytokeratin (green) and vimentin (red) were detected by FITC-conjugated mouse anti-cytokeratin and mouse anti-vimentin antibody, respectively. D . Quantification of cytokeratin, vimentin and desmin expression by flow cytometric analysis. About 99% of the HBCEC population stained positive for cytokeratin, whereof some were positive for both, cytokeratin and vimentin intermediate filament proteins. Expression of desmin intermediate filaments remained undetectable. The FITC-labeled IgG control and the secondary antibody control served as background staining balance.
Figure Legend Snippet: Characterization of primary human breast cancer epithelial cells (HBCEC) . A . Scanning electron micrographs of human breast cancer-derived cell cultures. The cells are squamous with many short and thin processes and grow upon each other. B . Ultrathin sections of two human breast cancer-derived cells, which partially overlap and are connected by desmosomes. The cells contain bundles of intermediate filaments and cytoplasmic vacuoles, whereas organelles are almost absent. In the right transmission micrograph, two squamous cell processes are connected by desmosomes and bundles of intermediate filaments are orientated in parallel to the cell surface. C . Immunofluorescence of intermediate filaments. Nuclei became visual using DAPI and the intermediate filament proteins cytokeratin (green) and vimentin (red) were detected by FITC-conjugated mouse anti-cytokeratin and mouse anti-vimentin antibody, respectively. D . Quantification of cytokeratin, vimentin and desmin expression by flow cytometric analysis. About 99% of the HBCEC population stained positive for cytokeratin, whereof some were positive for both, cytokeratin and vimentin intermediate filament proteins. Expression of desmin intermediate filaments remained undetectable. The FITC-labeled IgG control and the secondary antibody control served as background staining balance.

Techniques Used: Derivative Assay, Transmission Assay, Immunofluorescence, Expressing, Flow Cytometry, Staining, Labeling

33) Product Images from "MOLECULAR MECHANISMS OF PANCREATIC DYSFUNCTION INDUCED BY PROTEIN MALNUTRITION"

Article Title: MOLECULAR MECHANISMS OF PANCREATIC DYSFUNCTION INDUCED BY PROTEIN MALNUTRITION

Journal: Gastroenterology

doi: 10.1053/j.gastro.2009.04.058

Effect of dietary protein on pancreatic morphology. Electron microscopy of ultrathin sections stained with uranyl acetate and lead citrate. Mice were fed either 200 g/kg casein chow for 4 days (A), 0 g/kg casein chow 4 days (B), 0 g/kg casein chow for
Figure Legend Snippet: Effect of dietary protein on pancreatic morphology. Electron microscopy of ultrathin sections stained with uranyl acetate and lead citrate. Mice were fed either 200 g/kg casein chow for 4 days (A), 0 g/kg casein chow 4 days (B), 0 g/kg casein chow for

Techniques Used: Electron Microscopy, Staining, Mouse Assay

34) Product Images from "The ARF-Like GTPase ARFRP1 Is Essential for Lipid Droplet Growth and Is Involved in the Regulation of Lipolysis ▿"

Article Title: The ARF-Like GTPase ARFRP1 Is Essential for Lipid Droplet Growth and Is Involved in the Regulation of Lipolysis ▿

Journal: Molecular and Cellular Biology

doi: 10.1128/MCB.01269-09

Reduced size and defective fusion of lipid droplets in BAT of Arfrp1 ad −/− mice. (A) Ultrastructural analysis of BAT of 7-day-old Arfrp1 flox/flox and Arfrp1 ad −/− mice. Electron microscopic overview of ultrathin sections of BAT of 7-day-old Arfrp1 flox/flox and Arfrp1 ad −/− mice (left) and quantification and calculation of the number and volume of LDs (right). (B) Sections of BAT analyzed by electron microscopy at higher magnifications (white arrows indicate association of electron-dense droplets with large LD; black arrows indicate membranes which might represent extensions of ER).
Figure Legend Snippet: Reduced size and defective fusion of lipid droplets in BAT of Arfrp1 ad −/− mice. (A) Ultrastructural analysis of BAT of 7-day-old Arfrp1 flox/flox and Arfrp1 ad −/− mice. Electron microscopic overview of ultrathin sections of BAT of 7-day-old Arfrp1 flox/flox and Arfrp1 ad −/− mice (left) and quantification and calculation of the number and volume of LDs (right). (B) Sections of BAT analyzed by electron microscopy at higher magnifications (white arrows indicate association of electron-dense droplets with large LD; black arrows indicate membranes which might represent extensions of ER).

Techniques Used: Mouse Assay, Electron Microscopy

35) Product Images from "Urothelial Plaque Formation in Post-Golgi Compartments"

Article Title: Urothelial Plaque Formation in Post-Golgi Compartments

Journal: PLoS ONE

doi: 10.1371/journal.pone.0023636

Uroplakins are not accumulated in the Golgi cisternae, but in the post-Golgi compartments. A) Top-view on the umbrella cell immunolabelled with anti-GM130 (green) shows a GA network spread across the whole cytoplasm. B) Plaques of asymmetric thickened membrane are not seen in the Golgi cisternae. C) Inset shows higher magnification of GA part, which has membranes 8–9 nm thick. D and E) Optical sections created from electron tomogram reconstruction, with superimposed models of the GA. D) GA forms flattened, occasionally fenestrated cisternae of various lengths (violet). E) From the rims of trans -Golgi cisterne and from the trans -Golgi network emanate tubulo-vesicular extensions (gold; GA cisternae are violet). F) Double immunolabelling with anti-gianti antibody (green, arrows) and anti-AUM antibody against mature uroplakins (red), shows no colocalization in the umbrella cell. GA (arrows) lies lateral to the nucleus, while uroplakins are seen throughout the cytoplasm (*) and on the apical surface (arrow-head). G) Labelling of cryo-ultrathin sections with anti-AUM antibody is negative in the GA and positive in post-Golgi compartments (arrows). Legend: M – mitochondria; blue – nucleus (DAPI). Bars 10 µm in A, F, 200 nm in B, G. Tomogram for D, E was taken at 6500×.
Figure Legend Snippet: Uroplakins are not accumulated in the Golgi cisternae, but in the post-Golgi compartments. A) Top-view on the umbrella cell immunolabelled with anti-GM130 (green) shows a GA network spread across the whole cytoplasm. B) Plaques of asymmetric thickened membrane are not seen in the Golgi cisternae. C) Inset shows higher magnification of GA part, which has membranes 8–9 nm thick. D and E) Optical sections created from electron tomogram reconstruction, with superimposed models of the GA. D) GA forms flattened, occasionally fenestrated cisternae of various lengths (violet). E) From the rims of trans -Golgi cisterne and from the trans -Golgi network emanate tubulo-vesicular extensions (gold; GA cisternae are violet). F) Double immunolabelling with anti-gianti antibody (green, arrows) and anti-AUM antibody against mature uroplakins (red), shows no colocalization in the umbrella cell. GA (arrows) lies lateral to the nucleus, while uroplakins are seen throughout the cytoplasm (*) and on the apical surface (arrow-head). G) Labelling of cryo-ultrathin sections with anti-AUM antibody is negative in the GA and positive in post-Golgi compartments (arrows). Legend: M – mitochondria; blue – nucleus (DAPI). Bars 10 µm in A, F, 200 nm in B, G. Tomogram for D, E was taken at 6500×.

Techniques Used:

36) Product Images from "Unintended spread of a biosafety level 2 recombinant retrovirus"

Article Title: Unintended spread of a biosafety level 2 recombinant retrovirus

Journal: Retrovirology

doi: 10.1186/1742-4690-6-86

Electron microscopical analysis of viral contaminants . Viral particles from the supernatant of 293T cells were pelleted through a 20% sucrose cushion. Ultrathin sections of the fixed pellet show two types of particles resembling retroviruses. The more abundant one (*) exhibits a central electron-dense core, while the core of the other type of particles (→) is located excentrically. Scale bar: 100 nm.
Figure Legend Snippet: Electron microscopical analysis of viral contaminants . Viral particles from the supernatant of 293T cells were pelleted through a 20% sucrose cushion. Ultrathin sections of the fixed pellet show two types of particles resembling retroviruses. The more abundant one (*) exhibits a central electron-dense core, while the core of the other type of particles (→) is located excentrically. Scale bar: 100 nm.

Techniques Used:

37) Product Images from "Isolation of a Rickettsial Pathogen from a Non-Hematophagous Arthropod"

Article Title: Isolation of a Rickettsial Pathogen from a Non-Hematophagous Arthropod

Journal: PLoS ONE

doi: 10.1371/journal.pone.0016396

Electron micrographs of Rickettsia in booklice. (A) Rickettsiae free in the cytosol of gut epithelial cells. MV represents microvilli. (B) Higher magnification of Fig. 2A shows typical rickettsial ultrastructure: rickettsial cell wall including trilaminar cell wall membrane associated with the external surface microcapsule layer and an internal trilaminar cytoplasmic membrane (arrowheads), surrounded by an outermost “halo” zone (h). Solid arrow indicates a small vacuole inside rickettsial cytoplasm. (C) Rickettsiae (R) in ovary. Inset: higher magnification view of the boxed rickettsiae. (D) Ultrathin section of a mycetome-like, several cells of which contain large vacuoles tightly packed with rickettsiae of irregular shape with dense cytoplasm. Bar = 500 nm.
Figure Legend Snippet: Electron micrographs of Rickettsia in booklice. (A) Rickettsiae free in the cytosol of gut epithelial cells. MV represents microvilli. (B) Higher magnification of Fig. 2A shows typical rickettsial ultrastructure: rickettsial cell wall including trilaminar cell wall membrane associated with the external surface microcapsule layer and an internal trilaminar cytoplasmic membrane (arrowheads), surrounded by an outermost “halo” zone (h). Solid arrow indicates a small vacuole inside rickettsial cytoplasm. (C) Rickettsiae (R) in ovary. Inset: higher magnification view of the boxed rickettsiae. (D) Ultrathin section of a mycetome-like, several cells of which contain large vacuoles tightly packed with rickettsiae of irregular shape with dense cytoplasm. Bar = 500 nm.

Techniques Used:

38) Product Images from "Host-Dependent Trigger of Caspases and Apoptosis by Legionella pneumophila ▿ ▿ †"

Article Title: Host-Dependent Trigger of Caspases and Apoptosis by Legionella pneumophila ▿ ▿ †

Journal: Infection and Immunity

doi: 10.1128/IAI.00147-07

Transmission electron micrographs of the lungs of A/J mice infected with the dotA mutant of L. pneumophila . Representative TEM images of lung tissues of A/J mice infected with 10 6 CFU/mouse of the dotA mutant of L. pneumophila AA100 are shown. The black arrows indicate bacteria. The experiments were done in triplicate, using three mice for each time point, and the images are representative of 10 ultrathin sections from each animal.
Figure Legend Snippet: Transmission electron micrographs of the lungs of A/J mice infected with the dotA mutant of L. pneumophila . Representative TEM images of lung tissues of A/J mice infected with 10 6 CFU/mouse of the dotA mutant of L. pneumophila AA100 are shown. The black arrows indicate bacteria. The experiments were done in triplicate, using three mice for each time point, and the images are representative of 10 ultrathin sections from each animal.

Techniques Used: Transmission Assay, Mouse Assay, Infection, Mutagenesis, Transmission Electron Microscopy

Transmission electron micrographs of the lungs of A/J mice infected with L. pneumophila AA100. Representative TEM images of lung tissues of A/J mice infected with 10 6 CFU/mouse of L. pneumophila AA100 are shown. At 2 h after infection, the morphological changes were very similar to those for the uninfected lung tissues of the A/J mice. At 24 and 48 h postinfection, condensation of chromatin was evident in pulmonary cells. The black and white arrows indicate bacteria and the apoptotic nuclei, respectively. The experiments were done in triplicate, using three mice for each time point, and the images are representative of 10 ultrathin sections from each animal.
Figure Legend Snippet: Transmission electron micrographs of the lungs of A/J mice infected with L. pneumophila AA100. Representative TEM images of lung tissues of A/J mice infected with 10 6 CFU/mouse of L. pneumophila AA100 are shown. At 2 h after infection, the morphological changes were very similar to those for the uninfected lung tissues of the A/J mice. At 24 and 48 h postinfection, condensation of chromatin was evident in pulmonary cells. The black and white arrows indicate bacteria and the apoptotic nuclei, respectively. The experiments were done in triplicate, using three mice for each time point, and the images are representative of 10 ultrathin sections from each animal.

Techniques Used: Transmission Assay, Mouse Assay, Infection, Transmission Electron Microscopy

39) Product Images from "Expression of mesenchymal stem cell marker CD90 on dermal sheath cells of the anagen hair follicle in canine species"

Article Title: Expression of mesenchymal stem cell marker CD90 on dermal sheath cells of the anagen hair follicle in canine species

Journal: European Journal of Histochemistry : EJH

doi: 10.4081/ejh.2009.e19

The soprabulbar and bulbar region of the anagen hair follicle. The dermal sheath cells are located all along the hair follicle, are very thin and flattened with elongated nuclei. The cytoplasm appears as a threadlike frame that occasionally overlaps among adjacent cells (thin arrow). Semithin (a–c) and ultrathin sections (b–d). Arrows = dermal sheath cells; E = epithelial cells of the follicle; *basal membrane; F, collagen fibres. Bar =10 µm (a–c), 5 µm (b), 2 µm (d).
Figure Legend Snippet: The soprabulbar and bulbar region of the anagen hair follicle. The dermal sheath cells are located all along the hair follicle, are very thin and flattened with elongated nuclei. The cytoplasm appears as a threadlike frame that occasionally overlaps among adjacent cells (thin arrow). Semithin (a–c) and ultrathin sections (b–d). Arrows = dermal sheath cells; E = epithelial cells of the follicle; *basal membrane; F, collagen fibres. Bar =10 µm (a–c), 5 µm (b), 2 µm (d).

Techniques Used:

40) Product Images from "Identification, Subviral Localization, and Functional Characterization of the Pseudorabies Virus UL17 Protein"

Article Title: Identification, Subviral Localization, and Functional Characterization of the Pseudorabies Virus UL17 Protein

Journal:

doi: 10.1128/JVI.79.21.13442-13453.2005

Immunoelectron microscopy of PrV-Ka-infected cells. RK13 cells were infected with PrV-Ka as described above, and ultrathin sections were labeled with anti-UL17 serum (A to G) or anti-UL48 serum (H). Anti-rabbit sera conjugated with gold (10 nm [B, D,
Figure Legend Snippet: Immunoelectron microscopy of PrV-Ka-infected cells. RK13 cells were infected with PrV-Ka as described above, and ultrathin sections were labeled with anti-UL17 serum (A to G) or anti-UL48 serum (H). Anti-rabbit sera conjugated with gold (10 nm [B, D,

Techniques Used: Immuno-Electron Microscopy, Infection, Labeling

Related Articles

Staining:

Article Title: Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.) ▿ L.) ▿ †
Article Snippet: .. The ultrathin sections were mounted on grids, stained with Reynold's lead citrate solution and uranyl acetate, and visualized using a Philips CM100 electron microscope. .. The sequence of the 2,422-bp fragment containing a partial hrpS ORF, the intergenic region located between hrpS and hrpA , and the complete hrpA and hrpZ ORFs was deposited in GenBank under accession number .

Article Title: GRIM-19, a Cell Death Regulatory Protein, Is Essential for Assembly and Function of Mitochondrial Complex I
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with an EM208 transmission electron microscope (Philips Electron Optics). .. Blastocyst outgrowth was labeled as described ( ) by using a 5-bromo-2′-deoxyuridine (BrdU) Labeling and Detection Kit II (Roche).

Article Title: Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots
Article Snippet: .. Ultrathin sections were mounted in grids, stained using Reynold's lead citrate solution and uranyl acetate and visualized using a Philips CM100 electron microscope. ..

Microscopy:

Article Title: The Pseudorabies Virus UL11 Protein Is a Virion Component Involved in Secondary Envelopment in the Cytoplasm
Article Snippet: .. The counterstained ultrathin sections were analyzed with an electron microscope (Tecnai 12; Philips, Eindhoven, The Netherlands). .. For identification of the PrV UL11 gene product, the complete ORF was cloned into the prokaryotic expression vector pGEX-4T-1, expressed in E. coli as a GST fusion protein, and used for immunization of a rabbit.

Article Title: Fiberless Recombinant Adenoviruses: Virus Maturation and Infectivity in the Absence of Fiber
Article Snippet: .. Ultrathin sections were observed under a Philips CM120 Biotwin electron microscope at 120 kV. .. 293 or 293-Fb cells were infected with Ad-LacZ or Ad-LacZ/Fb° at an MOI of 2 IU/cell.

Article Title: Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.) ▿ L.) ▿ †
Article Snippet: .. The ultrathin sections were mounted on grids, stained with Reynold's lead citrate solution and uranyl acetate, and visualized using a Philips CM100 electron microscope. .. The sequence of the 2,422-bp fragment containing a partial hrpS ORF, the intergenic region located between hrpS and hrpA , and the complete hrpA and hrpZ ORFs was deposited in GenBank under accession number .

Article Title: The Capsid-Associated UL25 Protein of the Alphaherpesvirus Pseudorabies Virus Is Nonessential for Cleavage and Encapsidation of Genomic DNA but Is Required for Nuclear Egress of Capsids
Article Snippet: .. Counterstained ultrathin sections were analyzed with an electron microscope (Technai 12; Philips, The Netherlands). .. Immunolabeling was performed with the anti-UL25 serum and secondary gold-tagged anti-rabbit antibodies (GAR10, GAR 5; British Biocell International, Cambridge, United Kingdom) as previously described ( ).

Article Title: Effects of Multiple Deletions of Murein Hydrolases on Viability, Septum Cleavage, and Sensitivity to Large Toxic Molecules in Escherichia coli
Article Snippet: .. Ultrathin sections were viewed with a Philips CM10 electron microscope. .. For scanning electron microscopy the fixed cells were dehydrated in ethanol and critical point dried from CO2 .

Article Title: GRIM-19, a Cell Death Regulatory Protein, Is Essential for Assembly and Function of Mitochondrial Complex I
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with an EM208 transmission electron microscope (Philips Electron Optics). .. Blastocyst outgrowth was labeled as described ( ) by using a 5-bromo-2′-deoxyuridine (BrdU) Labeling and Detection Kit II (Roche).

Article Title: DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles
Article Snippet: .. Ultrathin sections of infected cells and virions were viewed with a Philips CM100 electron microscope. .. The A32 gene contains a typical late promoter consensus sequence and an ORF that predicts a nonmembrane protein of 34.4 kDa.

Article Title: Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots
Article Snippet: .. Ultrathin sections were mounted in grids, stained using Reynold's lead citrate solution and uranyl acetate and visualized using a Philips CM100 electron microscope. ..

Infection:

Article Title: DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles
Article Snippet: .. Ultrathin sections of infected cells and virions were viewed with a Philips CM100 electron microscope. .. The A32 gene contains a typical late promoter consensus sequence and an ORF that predicts a nonmembrane protein of 34.4 kDa.

Transmission Assay:

Article Title: GRIM-19, a Cell Death Regulatory Protein, Is Essential for Assembly and Function of Mitochondrial Complex I
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with an EM208 transmission electron microscope (Philips Electron Optics). .. Blastocyst outgrowth was labeled as described ( ) by using a 5-bromo-2′-deoxyuridine (BrdU) Labeling and Detection Kit II (Roche).

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    Philips Healthcare ultrathin sections
    Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and <t>ultrathin</t> sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.
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    Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and ultrathin sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.

    Journal: Journal of Virology

    Article Title: Fiberless Recombinant Adenoviruses: Virus Maturation and Infectivity in the Absence of Fiber

    doi:

    Figure Lengend Snippet: Electron microscopy analysis of 293 cells infected with fiberless viruses. 293 and 293-Fb cells were infected at an MOI of 10 IU/cell with Ad5, Ad-LacZ, and Ad-LacZ/Fb°max produced on 293-Fb cells. Infected cells were fixed with glutaraldehyde at 29 h postinfection, and ultrathin sections were stained with uranyl acetate. (A) Nuclear accumulation of Ad-LacZ particles in 293 cells. Magnification, ×20,000. (B) nuclear accumulation of Ad5 particles in 293-Fb cells. Magnification, ×20,000. (C to E) Infection of 293 (C) or 293-Fb (D and E) cells with Ad-LacZ/Fb°max leads to an important nuclear (C and D) and cytoplasmic (E) accumulation of fiberless virus particles. Such particles are often associated with unusual nuclear membrane structures (arrows) (C and D). Magnifications, ×20,000 (C and E) and ×26,000 (D). c, cytoplasm; n, nucleus; m, mitochondria; μ, micrometer.

    Article Snippet: Ultrathin sections were observed under a Philips CM120 Biotwin electron microscope at 120 kV.

    Techniques: Electron Microscopy, Infection, Produced, Staining

    Abnormal morphology and cellular distribution of mitochondria in the mutant blastocysts. Ultrathin sections of the blastocysts were examined with a transmission electron microscope. The mitochondria of the normal (A and C) and the mutant (B and D) cells are indicated by arrows. Scale bar = 1 μm.

    Journal: Molecular and Cellular Biology

    Article Title: GRIM-19, a Cell Death Regulatory Protein, Is Essential for Assembly and Function of Mitochondrial Complex I

    doi: 10.1128/MCB.24.19.8447-8456.2004

    Figure Lengend Snippet: Abnormal morphology and cellular distribution of mitochondria in the mutant blastocysts. Ultrathin sections of the blastocysts were examined with a transmission electron microscope. The mitochondria of the normal (A and C) and the mutant (B and D) cells are indicated by arrows. Scale bar = 1 μm.

    Article Snippet: Ultrathin sections were stained with uranyl acetate and lead citrate and examined with an EM208 transmission electron microscope (Philips Electron Optics).

    Techniques: Mutagenesis, Transmission Assay, Microscopy

    Electron microscopy of purified virus particles. Particles purified by sucrose gradient centrifugation from HeLa cells infected with WR or vA32i in the absence of IPTG were diluted, collected by high-speed centrifugation, fixed in glutaraldehyde, and embedded in Epon. Ultrathin sections were examined by electron microscopy.

    Journal: Journal of Virology

    Article Title: DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles

    doi:

    Figure Lengend Snippet: Electron microscopy of purified virus particles. Particles purified by sucrose gradient centrifugation from HeLa cells infected with WR or vA32i in the absence of IPTG were diluted, collected by high-speed centrifugation, fixed in glutaraldehyde, and embedded in Epon. Ultrathin sections were examined by electron microscopy.

    Article Snippet: Ultrathin sections of infected cells and virions were viewed with a Philips CM100 electron microscope.

    Techniques: Electron Microscopy, Purification, Gradient Centrifugation, Infection, Centrifugation

    Morphogenesis of mutant viruses. BS-C-1 cells were infected with vA32/A32i (A and C) or vA32i (B and D) in the absence of IPTG. After 24 h, the cells were fixed in glutaraldehyde and embedded in Epon, and then ultrathin sections were prepared for electron microscopy. Cr, crescents; Nu, nucleoids; IMV, intracellular mature virions; IV, immature virions; DIV, dense immature virions.

    Journal: Journal of Virology

    Article Title: DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles

    doi:

    Figure Lengend Snippet: Morphogenesis of mutant viruses. BS-C-1 cells were infected with vA32/A32i (A and C) or vA32i (B and D) in the absence of IPTG. After 24 h, the cells were fixed in glutaraldehyde and embedded in Epon, and then ultrathin sections were prepared for electron microscopy. Cr, crescents; Nu, nucleoids; IMV, intracellular mature virions; IV, immature virions; DIV, dense immature virions.

    Article Snippet: Ultrathin sections of infected cells and virions were viewed with a Philips CM100 electron microscope.

    Techniques: Mutagenesis, Infection, Electron Microscopy

    Transmission electron micrographs of ultrathin sections of knots induced by P. savastanoi pv. savastanoi NCPPB‐3335‐GFP at 35 dpi on in vitro olive plants. A. Ultrastructure of knot tissue showing parenchymatic‐like cells containing a fibrillar cytoplasm and irregular cell wall thickenings (arrowhead). Bacterial cells were visualized at intercellular spaces (black arrow). B. Pseudomonas savastanoi pv. savastanoi cells (black arrow) localized at the intercellular space of two host cells. A condensed cytoplasm (asterisk) and a degraded middle lamella are shown. C. Bacterial cells (black arrow) in contact with a primary cell wall in the process of degradation (arrowhead). D. Abnormal cell wall accumulations of host cells in close contact with degenerated cytoplasm (asterisks) and pathogen cells (black arrow). E. Rod‐shaped (black arrow) and irregular (open arrow) bacterial cells colonizing the extracellular space of a host cell showing degenerated organelles (asterisks). F. Group of P. savastanoi pv. savastanoi cells within a degenerated host cell. Bacterial cells are seen surrounded by an electrolucent halo and immersed in a fibroreticular matrix. G. Detail of (F), high‐magnification image of the bacterial surface releasing outer‐membrane vesicles (arrowheads). H. Detail of the fusion of outer‐membrane vesicles (arrowhead) to the fibroreticular matrix.

    Journal: Microbial biotechnology

    Article Title: Endopathogenic lifestyle of Pseudomonas savastanoi pv. savastanoi in olive knots

    doi: 10.1111/j.1751-7915.2009.00101.x

    Figure Lengend Snippet: Transmission electron micrographs of ultrathin sections of knots induced by P. savastanoi pv. savastanoi NCPPB‐3335‐GFP at 35 dpi on in vitro olive plants. A. Ultrastructure of knot tissue showing parenchymatic‐like cells containing a fibrillar cytoplasm and irregular cell wall thickenings (arrowhead). Bacterial cells were visualized at intercellular spaces (black arrow). B. Pseudomonas savastanoi pv. savastanoi cells (black arrow) localized at the intercellular space of two host cells. A condensed cytoplasm (asterisk) and a degraded middle lamella are shown. C. Bacterial cells (black arrow) in contact with a primary cell wall in the process of degradation (arrowhead). D. Abnormal cell wall accumulations of host cells in close contact with degenerated cytoplasm (asterisks) and pathogen cells (black arrow). E. Rod‐shaped (black arrow) and irregular (open arrow) bacterial cells colonizing the extracellular space of a host cell showing degenerated organelles (asterisks). F. Group of P. savastanoi pv. savastanoi cells within a degenerated host cell. Bacterial cells are seen surrounded by an electrolucent halo and immersed in a fibroreticular matrix. G. Detail of (F), high‐magnification image of the bacterial surface releasing outer‐membrane vesicles (arrowheads). H. Detail of the fusion of outer‐membrane vesicles (arrowhead) to the fibroreticular matrix.

    Article Snippet: Ultrathin sections were mounted in grids, stained using Reynold's lead citrate solution and uranyl acetate and visualized using a Philips CM100 electron microscope.

    Techniques: Transmission Assay, In Vitro