ultrathin sections  (Millipore)


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    Structured Review

    Millipore ultrathin sections
    A, Relationship of the synaptic junction area to target structure circumference for three FS and three LTS cells. The data were obtained from the 3-D reconstructions of serial <t>ultrathin</t> sections. Synapses on dendritic shafts are represented by filled
    Ultrathin Sections, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 154 article reviews
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    ultrathin sections - by Bioz Stars, 2020-08
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    Images

    1) Product Images from "Dependence of GABAergic Synaptic Areas on the Interneuron Type and Target Size"

    Article Title: Dependence of GABAergic Synaptic Areas on the Interneuron Type and Target Size

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.20-01-00375.2000

    A, Relationship of the synaptic junction area to target structure circumference for three FS and three LTS cells. The data were obtained from the 3-D reconstructions of serial ultrathin sections. Synapses on dendritic shafts are represented by filled
    Figure Legend Snippet: A, Relationship of the synaptic junction area to target structure circumference for three FS and three LTS cells. The data were obtained from the 3-D reconstructions of serial ultrathin sections. Synapses on dendritic shafts are represented by filled

    Techniques Used:

    2) Product Images from "A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure"

    Article Title: A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure

    Journal: Nature Communications

    doi: 10.1038/s41467-017-02768-7

    Different metal staining protocols make a difference in image quality of ultrathin sections, but images obtained with SEM or TEM are comparable. a – l Images of ultrathin sections of a mHMS, b TO, c TO with lead citrate (Pbc) section staining, d TO with uranyl acetate (ua) and lead citrate section staining, e TOLA, and f TOLA with lead citrate section staining on CNT-coated PET tape captured with a BSD. g – l Enlarged image showing synaptic junction in a – f , where postsynaptic spine heads are marked with an asterisk. Synaptic junction area is indicated with arrowheads. Scale in g , 0.25 µm, is for h – l , x , y . m – r Images of ultrathin sections of m mHMS, n TO, o TO with lead citrate section staining, p TO with uranyl acetate and lead citrate section staining, and q TOLA, and r TOLA with lead citrate section staining on cc-Kapton tape captured with a BSD. s – w Images of ultrathin sections of s mHMS, t TO, u TO with lead citrate section staining, v TO with uranyl acetate and lead citrate section staining, and w TOLA captured with TEM. Postsynaptic spine heads in v and w are marked with an asterisk. ( x and y ) Enlarged image showing synaptic junction of v and w . The synaptic junction area is marked with arrows. Scale in s , 0.5 µm, is for a – f , m – w
    Figure Legend Snippet: Different metal staining protocols make a difference in image quality of ultrathin sections, but images obtained with SEM or TEM are comparable. a – l Images of ultrathin sections of a mHMS, b TO, c TO with lead citrate (Pbc) section staining, d TO with uranyl acetate (ua) and lead citrate section staining, e TOLA, and f TOLA with lead citrate section staining on CNT-coated PET tape captured with a BSD. g – l Enlarged image showing synaptic junction in a – f , where postsynaptic spine heads are marked with an asterisk. Synaptic junction area is indicated with arrowheads. Scale in g , 0.25 µm, is for h – l , x , y . m – r Images of ultrathin sections of m mHMS, n TO, o TO with lead citrate section staining, p TO with uranyl acetate and lead citrate section staining, and q TOLA, and r TOLA with lead citrate section staining on cc-Kapton tape captured with a BSD. s – w Images of ultrathin sections of s mHMS, t TO, u TO with lead citrate section staining, v TO with uranyl acetate and lead citrate section staining, and w TOLA captured with TEM. Postsynaptic spine heads in v and w are marked with an asterisk. ( x and y ) Enlarged image showing synaptic junction of v and w . The synaptic junction area is marked with arrows. Scale in s , 0.5 µm, is for a – f , m – w

    Techniques Used: Staining, Transmission Electron Microscopy, Positron Emission Tomography

    Plasma glow discharge treatment prevents the generation of copious wrinkles on the sections collected on CNT tape. a CNT-coated PET tape consists of three layers. The middle layer is a 50 µm-thick core structure made of PET film. CNTs are buried in the over coat layer (2 µm-thick). A hard coat layer (2 µm-thick) is on the opposing side of the PET film. Both coats are composed of a non-disclosed polymer. b Surface resistance of the CNT tape is uniform. c Ultrathin sections on the CNT-coated PET tape show many wrinkles. d PWCA of the CNT tape is 79.5 degrees. e Possible mechanism of the plasma treatment effect on the collection of the ultrathin sections for the untreated tape with the steep PWCA may cause difficulty in section landing. f Ultrathin sections on the CNT-coated PET tape with plasma treatment show no wrinkles. g PWCA of the CNT-coated PET tape after the plasma discharge treatment becomes 7.4 degrees, which shows that the tape is very hydrophilic. h The shallow PWCA promotes a smooth landing of the ultrathin sections on the plasma-treated tape from the water surface. i , j The plasma treatment effect for no wrinkle generation lasts for 7 months ( i ) and 13 months ( j ). Scale, 100 µm. k Time course of the plasma treatment effect on the CNT-coated tape indicated by PWCA. Error bars denote SD. Scale in j is for c , f , i
    Figure Legend Snippet: Plasma glow discharge treatment prevents the generation of copious wrinkles on the sections collected on CNT tape. a CNT-coated PET tape consists of three layers. The middle layer is a 50 µm-thick core structure made of PET film. CNTs are buried in the over coat layer (2 µm-thick). A hard coat layer (2 µm-thick) is on the opposing side of the PET film. Both coats are composed of a non-disclosed polymer. b Surface resistance of the CNT tape is uniform. c Ultrathin sections on the CNT-coated PET tape show many wrinkles. d PWCA of the CNT tape is 79.5 degrees. e Possible mechanism of the plasma treatment effect on the collection of the ultrathin sections for the untreated tape with the steep PWCA may cause difficulty in section landing. f Ultrathin sections on the CNT-coated PET tape with plasma treatment show no wrinkles. g PWCA of the CNT-coated PET tape after the plasma discharge treatment becomes 7.4 degrees, which shows that the tape is very hydrophilic. h The shallow PWCA promotes a smooth landing of the ultrathin sections on the plasma-treated tape from the water surface. i , j The plasma treatment effect for no wrinkle generation lasts for 7 months ( i ) and 13 months ( j ). Scale, 100 µm. k Time course of the plasma treatment effect on the CNT-coated tape indicated by PWCA. Error bars denote SD. Scale in j is for c , f , i

    Techniques Used: Positron Emission Tomography

    Images of ultrathin sections on CNT-PET tape and cc-Kapton tape are comparable. a – d , Images of mHMS-treated brain tissue captured with the BSD at 5 keV, 3.2 µs dwell time ( a , c ) or the In-lens SE detector at 2 keV, 3.2 µs dwell time ( b , d ) on CNT-coated PET tape ( a , b ) or on cc-Kapton tape ( c , d ). Scale in d , 1 µm, is also for a – c
    Figure Legend Snippet: Images of ultrathin sections on CNT-PET tape and cc-Kapton tape are comparable. a – d , Images of mHMS-treated brain tissue captured with the BSD at 5 keV, 3.2 µs dwell time ( a , c ) or the In-lens SE detector at 2 keV, 3.2 µs dwell time ( b , d ) on CNT-coated PET tape ( a , b ) or on cc-Kapton tape ( c , d ). Scale in d , 1 µm, is also for a – c

    Techniques Used: Positron Emission Tomography

    Repeated image capturing does not substantially affect image quality. a Image of the mHMS ultrathin section of cortex captured with BSD for 3.2 µs dwell time, 3 nm pixel −1 , 60 μm aperture at 5 keV, 2.8 ke − nm −2 electron dose, where the 2048×2048 image size, 3.2 µs dwell time, 3 nm pixel −1 , 60 μm aperture at 5 keV had been captured one, 5, 10 and 20 times indicated by blue arrows from left to right. Scale, 3 µm, is also for b – d . b Image of the same location of a captured with an In-lens SE detector for 1.6 µs dwell time, 3 nm pixel −1 , 20 μm aperture at 3 keV. Darker squares correspond with the pre-imaged area. c The same area imaged with the ETD. The imaging times are indicated below the depression square. d The surface profile image. Depression depth is indicated above the depression square. e . Enlarged image of left green square in a . Arrows on the top left and middle left indicate the border of the once imaged area. Arrows on the top right and middle right indicate the border of the area imaged 5 times. f Enlarged image of right green square in a . Arrows on the top left and middle left indicate the border of the area imaged 10 times. Arrows on the top right and middle right indicate the border of the area imaged 20 times. g Enlarged image of left square in b . Arrows indicate the border shown in e . h Enlarged image of right square in b . Arrows indicate the border shown in f . Scale, 1 µm, is also for e – g . i Enlarged image of red rectangle in a . The number of imaging times are shown above the images. Scale, 1 µm. wd, working distance; dt, dwell time; ap, aperture
    Figure Legend Snippet: Repeated image capturing does not substantially affect image quality. a Image of the mHMS ultrathin section of cortex captured with BSD for 3.2 µs dwell time, 3 nm pixel −1 , 60 μm aperture at 5 keV, 2.8 ke − nm −2 electron dose, where the 2048×2048 image size, 3.2 µs dwell time, 3 nm pixel −1 , 60 μm aperture at 5 keV had been captured one, 5, 10 and 20 times indicated by blue arrows from left to right. Scale, 3 µm, is also for b – d . b Image of the same location of a captured with an In-lens SE detector for 1.6 µs dwell time, 3 nm pixel −1 , 20 μm aperture at 3 keV. Darker squares correspond with the pre-imaged area. c The same area imaged with the ETD. The imaging times are indicated below the depression square. d The surface profile image. Depression depth is indicated above the depression square. e . Enlarged image of left green square in a . Arrows on the top left and middle left indicate the border of the once imaged area. Arrows on the top right and middle right indicate the border of the area imaged 5 times. f Enlarged image of right green square in a . Arrows on the top left and middle left indicate the border of the area imaged 10 times. Arrows on the top right and middle right indicate the border of the area imaged 20 times. g Enlarged image of left square in b . Arrows indicate the border shown in e . h Enlarged image of right square in b . Arrows indicate the border shown in f . Scale, 1 µm, is also for e – g . i Enlarged image of red rectangle in a . The number of imaging times are shown above the images. Scale, 1 µm. wd, working distance; dt, dwell time; ap, aperture

    Techniques Used: Imaging

    3) Product Images from "The Arabidopsis NHL3 Gene Encodes a Plasma Membrane Protein and Its Overexpression Correlates with Increased Resistance to Pseudomonas syringae pv. tomato DC3000 1"

    Article Title: The Arabidopsis NHL3 Gene Encodes a Plasma Membrane Protein and Its Overexpression Correlates with Increased Resistance to Pseudomonas syringae pv. tomato DC3000 1

    Journal: Plant Physiology

    doi: 10.1104/pp.103.020438

    Immunolabeling localizes NHL3-HA to the periphery of the cell. Cross-sections of leaves from DEX-treated transgenic plants harboring the empty pTA7002 vector control (A) or NHL3-HA construct (B–E) were probed with an anti-HA antibody followed by an Alexa488-coupled (Molecular Probes, Eugene, OR) secondary antibody giving green signals (A–D) or by a secondary antibody conjugated with colloidal gold (E). In leaf sections of plants transformed with the empty vector control, only brownish autofluorescence is visible (A), whereas mesophyll (B) and epidermal cells (C) of plants expressing NHL3-HA show label. In both cases, cell walls do not exhibit label (arrow heads in B and C). Moreover, the label is restricted to the peripheral side of the cytoplasm as visible near the nucleus (arrows in C and D), which is visualized by the concomitant 4,6-diamidino-2-phenylindole (DAPI) staining (D). Immunogold labeling of ultrathin sections exhibits the majority of label at the periphery of the cytoplasm adjacent to the cell wall (arrows in E). cw, cell wall; v, vacuole; m, mitochondrion. Bars = 10 μm in A through D; bar = 0.5 μm in E.
    Figure Legend Snippet: Immunolabeling localizes NHL3-HA to the periphery of the cell. Cross-sections of leaves from DEX-treated transgenic plants harboring the empty pTA7002 vector control (A) or NHL3-HA construct (B–E) were probed with an anti-HA antibody followed by an Alexa488-coupled (Molecular Probes, Eugene, OR) secondary antibody giving green signals (A–D) or by a secondary antibody conjugated with colloidal gold (E). In leaf sections of plants transformed with the empty vector control, only brownish autofluorescence is visible (A), whereas mesophyll (B) and epidermal cells (C) of plants expressing NHL3-HA show label. In both cases, cell walls do not exhibit label (arrow heads in B and C). Moreover, the label is restricted to the peripheral side of the cytoplasm as visible near the nucleus (arrows in C and D), which is visualized by the concomitant 4,6-diamidino-2-phenylindole (DAPI) staining (D). Immunogold labeling of ultrathin sections exhibits the majority of label at the periphery of the cytoplasm adjacent to the cell wall (arrows in E). cw, cell wall; v, vacuole; m, mitochondrion. Bars = 10 μm in A through D; bar = 0.5 μm in E.

    Techniques Used: Immunolabeling, Transgenic Assay, Plasmid Preparation, Construct, Transformation Assay, Expressing, Staining, Labeling

    4) Product Images from "Intracellular Localization of the Pseudorabies Virus Large Tegument Protein pUL36 "

    Article Title: Intracellular Localization of the Pseudorabies Virus Large Tegument Protein pUL36

    Journal: Journal of Virology

    doi: 10.1128/JVI.01045-09

    Immunoelectron microscopy. RK13 cells were infected with PrV-Ka at an MOI of 1 and analyzed 14 h after infection. Ultrathin sections were immunogold labeled with anti-UL36-1 (A and B), anti-UL36-2 (C and D), anti-UL36-3 (E and F), anti-UL36-4 (G and H),
    Figure Legend Snippet: Immunoelectron microscopy. RK13 cells were infected with PrV-Ka at an MOI of 1 and analyzed 14 h after infection. Ultrathin sections were immunogold labeled with anti-UL36-1 (A and B), anti-UL36-2 (C and D), anti-UL36-3 (E and F), anti-UL36-4 (G and H),

    Techniques Used: Immuno-Electron Microscopy, Infection, Labeling

    5) Product Images from "Expression of mesenchymal stem cell marker CD90 on dermal sheath cells of the anagen hair follicle in canine species"

    Article Title: Expression of mesenchymal stem cell marker CD90 on dermal sheath cells of the anagen hair follicle in canine species

    Journal: European Journal of Histochemistry : EJH

    doi: 10.4081/ejh.2009.e19

    The soprabulbar and bulbar region of the anagen hair follicle. The dermal sheath cells are located all along the hair follicle, are very thin and flattened with elongated nuclei. The cytoplasm appears as a threadlike frame that occasionally overlaps among adjacent cells (thin arrow). Semithin (a–c) and ultrathin sections (b–d). Arrows = dermal sheath cells; E = epithelial cells of the follicle; *basal membrane; F, collagen fibres. Bar =10 µm (a–c), 5 µm (b), 2 µm (d).
    Figure Legend Snippet: The soprabulbar and bulbar region of the anagen hair follicle. The dermal sheath cells are located all along the hair follicle, are very thin and flattened with elongated nuclei. The cytoplasm appears as a threadlike frame that occasionally overlaps among adjacent cells (thin arrow). Semithin (a–c) and ultrathin sections (b–d). Arrows = dermal sheath cells; E = epithelial cells of the follicle; *basal membrane; F, collagen fibres. Bar =10 µm (a–c), 5 µm (b), 2 µm (d).

    Techniques Used:

    6) Product Images from "The exon junction complex factor Y14 is dynamic in the nucleus of the beetle Tribolium castaneum during late oogenesis"

    Article Title: The exon junction complex factor Y14 is dynamic in the nucleus of the beetle Tribolium castaneum during late oogenesis

    Journal: Molecular Cytogenetics

    doi: 10.1186/s13039-017-0342-4

    Ultrathin sections of T. castaneum GVs from oocytes injected with Y14–Myc mRNA after labeling with anti-Myc AB. a , Labeled nuclear body (SC35 domain) in the stage V oocyte. b , Similar nuclear body devoid of labels in the stage VII oocyte; a patch of labels ( arrowhead ) is visible and does not belong to the body. c , An overview of the fragment of karyosphere (ch, chromatin) and its capsule (kc); labels masking the fibrillar material in the perichromatin region are marked by arrows. d , The fragment of the karyosphere capsule; a patch of labels does not correspond SC35 domain. e , Anti-Y14–Myc labels near the nuclear envelope (ne); GV, germinal vesicle (oocyte nucleus); oo, ooplasm. Bars represent 0.5 μm in a — c , e and 200 nm in d
    Figure Legend Snippet: Ultrathin sections of T. castaneum GVs from oocytes injected with Y14–Myc mRNA after labeling with anti-Myc AB. a , Labeled nuclear body (SC35 domain) in the stage V oocyte. b , Similar nuclear body devoid of labels in the stage VII oocyte; a patch of labels ( arrowhead ) is visible and does not belong to the body. c , An overview of the fragment of karyosphere (ch, chromatin) and its capsule (kc); labels masking the fibrillar material in the perichromatin region are marked by arrows. d , The fragment of the karyosphere capsule; a patch of labels does not correspond SC35 domain. e , Anti-Y14–Myc labels near the nuclear envelope (ne); GV, germinal vesicle (oocyte nucleus); oo, ooplasm. Bars represent 0.5 μm in a — c , e and 200 nm in d

    Techniques Used: Injection, Labeling

    7) Product Images from "A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure"

    Article Title: A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure

    Journal: Nature Communications

    doi: 10.1038/s41467-017-02768-7

    Different metal staining protocols make a difference in image quality of ultrathin sections, but images obtained with SEM or TEM are comparable. a – l Images of ultrathin sections of a mHMS, b TO, c TO with lead citrate (Pbc) section staining, d TO with uranyl acetate (ua) and lead citrate section staining, e TOLA, and f TOLA with lead citrate section staining on CNT-coated PET tape captured with a BSD. g – l Enlarged image showing synaptic junction in a – f , where postsynaptic spine heads are marked with an asterisk. Synaptic junction area is indicated with arrowheads. Scale in g , 0.25 µm, is for h – l , x , y . m – r Images of ultrathin sections of m mHMS, n TO, o TO with lead citrate section staining, p TO with uranyl acetate and lead citrate section staining, and q TOLA, and r TOLA with lead citrate section staining on cc-Kapton tape captured with a BSD. s – w Images of ultrathin sections of s mHMS, t TO, u TO with lead citrate section staining, v TO with uranyl acetate and lead citrate section staining, and w TOLA captured with TEM. Postsynaptic spine heads in v and w are marked with an asterisk. ( x and y ) Enlarged image showing synaptic junction of v and w . The synaptic junction area is marked with arrows. Scale in s , 0.5 µm, is for a – f , m – w
    Figure Legend Snippet: Different metal staining protocols make a difference in image quality of ultrathin sections, but images obtained with SEM or TEM are comparable. a – l Images of ultrathin sections of a mHMS, b TO, c TO with lead citrate (Pbc) section staining, d TO with uranyl acetate (ua) and lead citrate section staining, e TOLA, and f TOLA with lead citrate section staining on CNT-coated PET tape captured with a BSD. g – l Enlarged image showing synaptic junction in a – f , where postsynaptic spine heads are marked with an asterisk. Synaptic junction area is indicated with arrowheads. Scale in g , 0.25 µm, is for h – l , x , y . m – r Images of ultrathin sections of m mHMS, n TO, o TO with lead citrate section staining, p TO with uranyl acetate and lead citrate section staining, and q TOLA, and r TOLA with lead citrate section staining on cc-Kapton tape captured with a BSD. s – w Images of ultrathin sections of s mHMS, t TO, u TO with lead citrate section staining, v TO with uranyl acetate and lead citrate section staining, and w TOLA captured with TEM. Postsynaptic spine heads in v and w are marked with an asterisk. ( x and y ) Enlarged image showing synaptic junction of v and w . The synaptic junction area is marked with arrows. Scale in s , 0.5 µm, is for a – f , m – w

    Techniques Used: Staining, Transmission Electron Microscopy, Positron Emission Tomography

    Plasma glow discharge treatment prevents the generation of copious wrinkles on the sections collected on CNT tape. a CNT-coated PET tape consists of three layers. The middle layer is a 50 µm-thick core structure made of PET film. CNTs are buried in the over coat layer (2 µm-thick). A hard coat layer (2 µm-thick) is on the opposing side of the PET film. Both coats are composed of a non-disclosed polymer. b Surface resistance of the CNT tape is uniform. c Ultrathin sections on the CNT-coated PET tape show many wrinkles. d PWCA of the CNT tape is 79.5 degrees. e Possible mechanism of the plasma treatment effect on the collection of the ultrathin sections for the untreated tape with the steep PWCA may cause difficulty in section landing. f Ultrathin sections on the CNT-coated PET tape with plasma treatment show no wrinkles. g PWCA of the CNT-coated PET tape after the plasma discharge treatment becomes 7.4 degrees, which shows that the tape is very hydrophilic. h The shallow PWCA promotes a smooth landing of the ultrathin sections on the plasma-treated tape from the water surface. i , j The plasma treatment effect for no wrinkle generation lasts for 7 months ( i ) and 13 months ( j ). Scale, 100 µm. k Time course of the plasma treatment effect on the CNT-coated tape indicated by PWCA. Error bars denote SD. Scale in j is for c , f , i
    Figure Legend Snippet: Plasma glow discharge treatment prevents the generation of copious wrinkles on the sections collected on CNT tape. a CNT-coated PET tape consists of three layers. The middle layer is a 50 µm-thick core structure made of PET film. CNTs are buried in the over coat layer (2 µm-thick). A hard coat layer (2 µm-thick) is on the opposing side of the PET film. Both coats are composed of a non-disclosed polymer. b Surface resistance of the CNT tape is uniform. c Ultrathin sections on the CNT-coated PET tape show many wrinkles. d PWCA of the CNT tape is 79.5 degrees. e Possible mechanism of the plasma treatment effect on the collection of the ultrathin sections for the untreated tape with the steep PWCA may cause difficulty in section landing. f Ultrathin sections on the CNT-coated PET tape with plasma treatment show no wrinkles. g PWCA of the CNT-coated PET tape after the plasma discharge treatment becomes 7.4 degrees, which shows that the tape is very hydrophilic. h The shallow PWCA promotes a smooth landing of the ultrathin sections on the plasma-treated tape from the water surface. i , j The plasma treatment effect for no wrinkle generation lasts for 7 months ( i ) and 13 months ( j ). Scale, 100 µm. k Time course of the plasma treatment effect on the CNT-coated tape indicated by PWCA. Error bars denote SD. Scale in j is for c , f , i

    Techniques Used: Positron Emission Tomography

    Images of ultrathin sections on CNT-PET tape and cc-Kapton tape are comparable. a – d , Images of mHMS-treated brain tissue captured with the BSD at 5 keV, 3.2 µs dwell time ( a , c ) or the In-lens SE detector at 2 keV, 3.2 µs dwell time ( b , d ) on CNT-coated PET tape ( a , b ) or on cc-Kapton tape ( c , d ). Scale in d , 1 µm, is also for a – c
    Figure Legend Snippet: Images of ultrathin sections on CNT-PET tape and cc-Kapton tape are comparable. a – d , Images of mHMS-treated brain tissue captured with the BSD at 5 keV, 3.2 µs dwell time ( a , c ) or the In-lens SE detector at 2 keV, 3.2 µs dwell time ( b , d ) on CNT-coated PET tape ( a , b ) or on cc-Kapton tape ( c , d ). Scale in d , 1 µm, is also for a – c

    Techniques Used: Positron Emission Tomography

    Repeated image capturing does not substantially affect image quality. a Image of the mHMS ultrathin section of cortex captured with BSD for 3.2 µs dwell time, 3 nm pixel −1 , 60 μm aperture at 5 keV, 2.8 ke − nm −2 electron dose, where the 2048×2048 image size, 3.2 µs dwell time, 3 nm pixel −1 , 60 μm aperture at 5 keV had been captured one, 5, 10 and 20 times indicated by blue arrows from left to right. Scale, 3 µm, is also for b – d . b Image of the same location of a captured with an In-lens SE detector for 1.6 µs dwell time, 3 nm pixel −1 , 20 μm aperture at 3 keV. Darker squares correspond with the pre-imaged area. c The same area imaged with the ETD. The imaging times are indicated below the depression square. d The surface profile image. Depression depth is indicated above the depression square. e . Enlarged image of left green square in a . Arrows on the top left and middle left indicate the border of the once imaged area. Arrows on the top right and middle right indicate the border of the area imaged 5 times. f Enlarged image of right green square in a . Arrows on the top left and middle left indicate the border of the area imaged 10 times. Arrows on the top right and middle right indicate the border of the area imaged 20 times. g Enlarged image of left square in b . Arrows indicate the border shown in e . h Enlarged image of right square in b . Arrows indicate the border shown in f . Scale, 1 µm, is also for e – g . i Enlarged image of red rectangle in a . The number of imaging times are shown above the images. Scale, 1 µm. wd, working distance; dt, dwell time; ap, aperture
    Figure Legend Snippet: Repeated image capturing does not substantially affect image quality. a Image of the mHMS ultrathin section of cortex captured with BSD for 3.2 µs dwell time, 3 nm pixel −1 , 60 μm aperture at 5 keV, 2.8 ke − nm −2 electron dose, where the 2048×2048 image size, 3.2 µs dwell time, 3 nm pixel −1 , 60 μm aperture at 5 keV had been captured one, 5, 10 and 20 times indicated by blue arrows from left to right. Scale, 3 µm, is also for b – d . b Image of the same location of a captured with an In-lens SE detector for 1.6 µs dwell time, 3 nm pixel −1 , 20 μm aperture at 3 keV. Darker squares correspond with the pre-imaged area. c The same area imaged with the ETD. The imaging times are indicated below the depression square. d The surface profile image. Depression depth is indicated above the depression square. e . Enlarged image of left green square in a . Arrows on the top left and middle left indicate the border of the once imaged area. Arrows on the top right and middle right indicate the border of the area imaged 5 times. f Enlarged image of right green square in a . Arrows on the top left and middle left indicate the border of the area imaged 10 times. Arrows on the top right and middle right indicate the border of the area imaged 20 times. g Enlarged image of left square in b . Arrows indicate the border shown in e . h Enlarged image of right square in b . Arrows indicate the border shown in f . Scale, 1 µm, is also for e – g . i Enlarged image of red rectangle in a . The number of imaging times are shown above the images. Scale, 1 µm. wd, working distance; dt, dwell time; ap, aperture

    Techniques Used: Imaging

    8) Product Images from "Structural and molecular features of intestinal strictures in rats with Crohn's-like disease"

    Article Title: Structural and molecular features of intestinal strictures in rats with Crohn's-like disease

    Journal: World Journal of Gastroenterology

    doi: 10.3748/wjg.v22.i22.5154

    Ultrastructural alterations within the colon of control animals and in rats treated three times with 2,4,6-trinitrobenzenesulfonic acid. Excess deposition of extracellular matrix (ECM) was observed within the smooth muscle layers in the ultrathin sections derived from the strictured region (A); Electronmicroscopic morphometry revealed that the distance between adjacent smooth muscle cells (SMCs) was significant larger in the strictured gut wall of the 2,4,6-trinitrobenzenesulfonic acid (TNBS)-treated rats (S) as compared with the gut wall of the control rats (C) on day 90 (B); A further significant increase in the mean separation distance of the SMCs was recorded on day 120 post-TNBS treatment (B). Data are expressed as mean ± SE. b P
    Figure Legend Snippet: Ultrastructural alterations within the colon of control animals and in rats treated three times with 2,4,6-trinitrobenzenesulfonic acid. Excess deposition of extracellular matrix (ECM) was observed within the smooth muscle layers in the ultrathin sections derived from the strictured region (A); Electronmicroscopic morphometry revealed that the distance between adjacent smooth muscle cells (SMCs) was significant larger in the strictured gut wall of the 2,4,6-trinitrobenzenesulfonic acid (TNBS)-treated rats (S) as compared with the gut wall of the control rats (C) on day 90 (B); A further significant increase in the mean separation distance of the SMCs was recorded on day 120 post-TNBS treatment (B). Data are expressed as mean ± SE. b P

    Techniques Used: Derivative Assay

    9) Product Images from "Isolation of an archaeon at the prokaryote–eukaryote interface"

    Article Title: Isolation of an archaeon at the prokaryote–eukaryote interface

    Journal: Nature

    doi: 10.1038/s41586-019-1916-6

    Other representative photomicrographs of MK-D1 cultures and Methanobacterium sp. strain MO-MB1. a , b , Fluorescence images of cells from enrichment cultures after 8 ( a ) and 11 ( b ) transfers stained with DAPI (violet) and hybridized with nucleotide probes that target MK-D1 (green) and Bacteria (red). The images are different fields of view to those shown in Fig. 1b, c , which were taken at the same time. c , A fluorescence image of cells in the enrichments after 11 transfers hybridized with nucleotide probes that target MK-D1 (green) and Archaea (but with one mismatch against MK-D1; red). Large and irregular coccoid-shaped cells stained by only ARC915 are probably Methanogenium . d , e , Dividing cells of MK-D1 with a bleb. The top-right inset image in e shows a magnification of the bleb. f , g , Cryo-EM images of MK-D1 cells and large membrane vesicles (white arrows). h , i , Ultrathin sections of MK-D1 cells with a membrane vesicle. The image i shows a magnified image of h . j , k , SEM images of MK-D1 cells with protrusions. l , Ultrathin section of a MK-D1 cell with a protrusion. m , n , Photomicrographs of pure culture of Methanobacterium sp. strain MO-MB1 cells stained with SYBR Green I. Phase-contrast ( m ) and fluorescence ( n ) images of the same field are shown. a , b , The FISH experiments were performed three times with similar results. d , e , j , k , The SEM images are representative of n = 122 recorded images that were obtained from four independent observations from four culture samples. The lipid composition experiments were repeated twice and gave similar results. f , g , The cryo-EM images are representative of n = 14 recorded images that were taken from two independent observations from two culture samples. h , i , l , The ultrathin-section images are representative of n = 131 recorded images that were obtained from six independent observations from six culture samples. m , n , The SYBR Green I staining experiment was performed once, but all 10 recorded images showed similar results. Detailed iTAG analyses of cultures are shown in Supplementary Table 1 .
    Figure Legend Snippet: Other representative photomicrographs of MK-D1 cultures and Methanobacterium sp. strain MO-MB1. a , b , Fluorescence images of cells from enrichment cultures after 8 ( a ) and 11 ( b ) transfers stained with DAPI (violet) and hybridized with nucleotide probes that target MK-D1 (green) and Bacteria (red). The images are different fields of view to those shown in Fig. 1b, c , which were taken at the same time. c , A fluorescence image of cells in the enrichments after 11 transfers hybridized with nucleotide probes that target MK-D1 (green) and Archaea (but with one mismatch against MK-D1; red). Large and irregular coccoid-shaped cells stained by only ARC915 are probably Methanogenium . d , e , Dividing cells of MK-D1 with a bleb. The top-right inset image in e shows a magnification of the bleb. f , g , Cryo-EM images of MK-D1 cells and large membrane vesicles (white arrows). h , i , Ultrathin sections of MK-D1 cells with a membrane vesicle. The image i shows a magnified image of h . j , k , SEM images of MK-D1 cells with protrusions. l , Ultrathin section of a MK-D1 cell with a protrusion. m , n , Photomicrographs of pure culture of Methanobacterium sp. strain MO-MB1 cells stained with SYBR Green I. Phase-contrast ( m ) and fluorescence ( n ) images of the same field are shown. a , b , The FISH experiments were performed three times with similar results. d , e , j , k , The SEM images are representative of n = 122 recorded images that were obtained from four independent observations from four culture samples. The lipid composition experiments were repeated twice and gave similar results. f , g , The cryo-EM images are representative of n = 14 recorded images that were taken from two independent observations from two culture samples. h , i , l , The ultrathin-section images are representative of n = 131 recorded images that were obtained from six independent observations from six culture samples. m , n , The SYBR Green I staining experiment was performed once, but all 10 recorded images showed similar results. Detailed iTAG analyses of cultures are shown in Supplementary Table 1 .

    Techniques Used: Fluorescence, Staining, SYBR Green Assay, Fluorescence In Situ Hybridization

    Microscopy characterization and lipid composition of MK-D1. a – c , SEM images of MK-D1. Single cell ( a ), aggregated cells covered with EPS-like materials ( b ) and a dividing cell with polar chains of blebs ( c ). d , Cryo-electron tomography image of MK-D1. The top-right inset image shows a magnification of the boxed area to show the cell envelope structure. e , Cryo-EM image of large membrane vesicles attached to and surrounding MK-D1 cells. f , Ultrathin section of an MK-D1 cell and a membrane vesicle. The bottom-right inset image shows a magnified view of the membrane vesicle. g , h , SEM images of MK-D1 cells producing long branching ( g ) and straight ( h ) membrane protrusions. i , Ultrathin section of a MK-D1 cell with protrusions. j , A total ion chromatogram of gas chromatography–mass spectrometry (GC–MS) for lipids extracted from a highly purified MK-D1 culture. The chemical structures of isoprenoids and their relative compositions are also shown (Supplementary Fig. 2 ). Scale bars, 1 μm ( b , c , g , h ), 500 nm ( a , d , e , i ) and 200 nm ( f ). a – c , g , h , SEM images are representative of n = 122 recorded images that were obtained from four independent observations from four culture samples. d , e , Cryo-EM images are representative of n = 14 recorded images that were taken from two independent observations from two culture samples. f , i , The ultrathin section images are representative of n = 131 recorded images that were obtained from six independent observations from six culture samples. White arrows in the images indicate large membrane vesicles. The lipid composition experiments were repeated twice and gave similar results. Detailed iTAG-based community compositions of the cultures are shown in Supplementary Table 1 .
    Figure Legend Snippet: Microscopy characterization and lipid composition of MK-D1. a – c , SEM images of MK-D1. Single cell ( a ), aggregated cells covered with EPS-like materials ( b ) and a dividing cell with polar chains of blebs ( c ). d , Cryo-electron tomography image of MK-D1. The top-right inset image shows a magnification of the boxed area to show the cell envelope structure. e , Cryo-EM image of large membrane vesicles attached to and surrounding MK-D1 cells. f , Ultrathin section of an MK-D1 cell and a membrane vesicle. The bottom-right inset image shows a magnified view of the membrane vesicle. g , h , SEM images of MK-D1 cells producing long branching ( g ) and straight ( h ) membrane protrusions. i , Ultrathin section of a MK-D1 cell with protrusions. j , A total ion chromatogram of gas chromatography–mass spectrometry (GC–MS) for lipids extracted from a highly purified MK-D1 culture. The chemical structures of isoprenoids and their relative compositions are also shown (Supplementary Fig. 2 ). Scale bars, 1 μm ( b , c , g , h ), 500 nm ( a , d , e , i ) and 200 nm ( f ). a – c , g , h , SEM images are representative of n = 122 recorded images that were obtained from four independent observations from four culture samples. d , e , Cryo-EM images are representative of n = 14 recorded images that were taken from two independent observations from two culture samples. f , i , The ultrathin section images are representative of n = 131 recorded images that were obtained from six independent observations from six culture samples. White arrows in the images indicate large membrane vesicles. The lipid composition experiments were repeated twice and gave similar results. Detailed iTAG-based community compositions of the cultures are shown in Supplementary Table 1 .

    Techniques Used: Microscopy, Gas Chromatography, Mass Spectrometry, Gas Chromatography-Mass Spectrometry, Purification

    10) Product Images from "Compensatory redistribution of neuroligins and N-cadherin following deletion of synaptic ?1-integrin"

    Article Title: Compensatory redistribution of neuroligins and N-cadherin following deletion of synaptic ?1-integrin

    Journal: The Journal of comparative neurology

    doi: 10.1002/cne.23027

    Synaptic distribution of β1-integrin labeling Immunogold labeling for β1-integrins in ultrathin sections using monoclonal antibody N29 (A, B) or p4C10 (C). Presynaptic terminals are shaded pink, and postsynaptic terminals, green. Gold particles are most commonly clustered at synaptic clefts (arrows) with a bias toward the postsynaptic density. Occasional particles are also found presynaptically (arrowheads in A). Dot plot (D) shows the distribution of β1-integrin immunogold labeling at synapses in all three control mice with respect to the peripheral edge (0) and center (0.5). Each synapse has been plotted along a line on the y axis. There is a distribution bias toward synapse centers. Magnification bar (A–C) = 500nm.
    Figure Legend Snippet: Synaptic distribution of β1-integrin labeling Immunogold labeling for β1-integrins in ultrathin sections using monoclonal antibody N29 (A, B) or p4C10 (C). Presynaptic terminals are shaded pink, and postsynaptic terminals, green. Gold particles are most commonly clustered at synaptic clefts (arrows) with a bias toward the postsynaptic density. Occasional particles are also found presynaptically (arrowheads in A). Dot plot (D) shows the distribution of β1-integrin immunogold labeling at synapses in all three control mice with respect to the peripheral edge (0) and center (0.5). Each synapse has been plotted along a line on the y axis. There is a distribution bias toward synapse centers. Magnification bar (A–C) = 500nm.

    Techniques Used: Labeling, Mouse Assay

    11) Product Images from "Localization of Nopp140 within mammalian cells during interphase and mitosis"

    Article Title: Localization of Nopp140 within mammalian cells during interphase and mitosis

    Journal: Histochemistry and cell biology

    doi: 10.1007/s00418-009-0599-8

    Immunoelectron microscopy localization of Nopp140 (a), fibrillarin (b) and nucleolin (c) within the nucleolus in cells treated with actinomycin D. Ultrathin sections of ELT cells, treated for 2 h with 0.05 μg/ml actinomycin D, were immunolabelled with anti-Nopp140 (RE10, a), anti-fibrillarin (b) and anti-nucleolin (c) antibodies. (a): in the segregated nucleolus, labelling was clearly evidenced over the FCs and the DFC, while the GC displayed only a few rare gold particles. (b): the DFC of the segregated nucleolus is the only compartment labelled by anti-fibrillarin antibodies. No labelling was observed over the FCs and the GC. (c): both the DFC and the GC were labelled by anti-nucleolin antibodies, while the FC was devoid of particles. Bars are 0.2 μm
    Figure Legend Snippet: Immunoelectron microscopy localization of Nopp140 (a), fibrillarin (b) and nucleolin (c) within the nucleolus in cells treated with actinomycin D. Ultrathin sections of ELT cells, treated for 2 h with 0.05 μg/ml actinomycin D, were immunolabelled with anti-Nopp140 (RE10, a), anti-fibrillarin (b) and anti-nucleolin (c) antibodies. (a): in the segregated nucleolus, labelling was clearly evidenced over the FCs and the DFC, while the GC displayed only a few rare gold particles. (b): the DFC of the segregated nucleolus is the only compartment labelled by anti-fibrillarin antibodies. No labelling was observed over the FCs and the GC. (c): both the DFC and the GC were labelled by anti-nucleolin antibodies, while the FC was devoid of particles. Bars are 0.2 μm

    Techniques Used: Immuno-Electron Microscopy

    12) Product Images from "Human adipose-derived mesenchymal stem cell-conditioned medium ameliorates polyneuropathy and foot ulceration in diabetic BKS db/db mice"

    Article Title: Human adipose-derived mesenchymal stem cell-conditioned medium ameliorates polyneuropathy and foot ulceration in diabetic BKS db/db mice

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-020-01680-0

    AD-MSC-conditioned medium administration recovers sciatic nerve ultrastructure in diabetic mice. a Representative bright-field images of semithin sections of sciatic nerves obtained from 26-week-old non-diabetic and diabetic mice treated with vehicle, conditioned medium derived from non-preconditioned AD-MSCs, or conditioned medium derived from DFX-preconditioned AD-MSCs. Myelinated fibers were stained with toluidine blue. b Frequency distribution histogram of myelinated fibers. c Representative electron microscopy images of ultrathin sections of sciatic nerves. Asterisks indicate fibers with disrupted myelin sheath, and arrows indicate the separation of shrunken axon from myelin sheath. d G-ratio quantification for small caliber (
    Figure Legend Snippet: AD-MSC-conditioned medium administration recovers sciatic nerve ultrastructure in diabetic mice. a Representative bright-field images of semithin sections of sciatic nerves obtained from 26-week-old non-diabetic and diabetic mice treated with vehicle, conditioned medium derived from non-preconditioned AD-MSCs, or conditioned medium derived from DFX-preconditioned AD-MSCs. Myelinated fibers were stained with toluidine blue. b Frequency distribution histogram of myelinated fibers. c Representative electron microscopy images of ultrathin sections of sciatic nerves. Asterisks indicate fibers with disrupted myelin sheath, and arrows indicate the separation of shrunken axon from myelin sheath. d G-ratio quantification for small caliber (

    Techniques Used: Mouse Assay, Derivative Assay, Staining, Electron Microscopy

    13) Product Images from "Nitrosomonas stercoris sp. nov., a Chemoautotrophic Ammonia-Oxidizing Bacterium Tolerant of High Ammonium Isolated from Composted Cattle Manure"

    Article Title: Nitrosomonas stercoris sp. nov., a Chemoautotrophic Ammonia-Oxidizing Bacterium Tolerant of High Ammonium Isolated from Composted Cattle Manure

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME15072

    Colonies and cell morphology of Nitrosomonas stercoris KYUHI-S T . (A) Colonies of strain KYUHI-S T on the gellan gum plate after an 87-d incubation. (B) Scanning electron micrograph of KYUHI-S T cells. Bar=1,000 nm. (C) Transmission electron micrographs of negatively stained strain KYUHI-S T . Bar=500 nm. (D) Transmission electron micrographs of ultrathin sections of strain KYUHI-S T . Black arrow shows the intracytoplasmic membrane within the periplasm. Bar=500 nm.
    Figure Legend Snippet: Colonies and cell morphology of Nitrosomonas stercoris KYUHI-S T . (A) Colonies of strain KYUHI-S T on the gellan gum plate after an 87-d incubation. (B) Scanning electron micrograph of KYUHI-S T cells. Bar=1,000 nm. (C) Transmission electron micrographs of negatively stained strain KYUHI-S T . Bar=500 nm. (D) Transmission electron micrographs of ultrathin sections of strain KYUHI-S T . Black arrow shows the intracytoplasmic membrane within the periplasm. Bar=500 nm.

    Techniques Used: Incubation, Transmission Assay, Staining

    14) Product Images from "Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development"

    Article Title: Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0046542

    Ultrathin sections of growing oocyte stained with RA47 (immunogold). a - mesogleal cell in adult medusa mesoglea; b – mesoglea embedded vegetative pole of the IV stage oocyte. 10 nm gold-conjugated secondary antibody used (Sigma, final dilution 1∶50). Symbols are the same as on fig. 4 and in addition: mc - mesogleal cell, Y – yolk granules, om – oocytes' basal membrane. White arrows indicate cistern-like structures in the lumen between oocyte and germinal epithelium. Letters with index (i.e. a′, c′ etc) indicate images of the high magnification i.e. parts from images with correspondent letters. Scale bar – 5 µm for a and a′, c and c′, d and e. Scale bar – 2 µm for b, d′ and d″, e′ and e″.
    Figure Legend Snippet: Ultrathin sections of growing oocyte stained with RA47 (immunogold). a - mesogleal cell in adult medusa mesoglea; b – mesoglea embedded vegetative pole of the IV stage oocyte. 10 nm gold-conjugated secondary antibody used (Sigma, final dilution 1∶50). Symbols are the same as on fig. 4 and in addition: mc - mesogleal cell, Y – yolk granules, om – oocytes' basal membrane. White arrows indicate cistern-like structures in the lumen between oocyte and germinal epithelium. Letters with index (i.e. a′, c′ etc) indicate images of the high magnification i.e. parts from images with correspondent letters. Scale bar – 5 µm for a and a′, c and c′, d and e. Scale bar – 2 µm for b, d′ and d″, e′ and e″.

    Techniques Used: Staining

    15) Product Images from "Important Factors for the Three-Dimensional Reconstruction of Neuronal Structures from Serial Ultrathin Sections"

    Article Title: Important Factors for the Three-Dimensional Reconstruction of Neuronal Structures from Serial Ultrathin Sections

    Journal: Frontiers in Neural Circuits

    doi: 10.3389/neuro.04.004.2009

    Serial ultrathin sections containing synapses with cleft plane cut in parallel or obliquely . (A–E) A synaptic contact with a cleft plane cut in parallel to the section plane is identified in the successive serial sections. In the first and second sections, many synaptic vesicles are found in the presynaptic bouton ( A, B , asterisk). The next section contains only a few vesicles and many round-shaped electron dense spots of about 35 nm diameter ( C , asterisk), that are likely to correspond to the pre-synaptic grid. The fourth section contains electron dense objects indicative of a PSD ( D , black arrow). In the fifth section, we observe a small part of the electron dense objects and the cytoplasm of the postsynaptic dendrite ( E , black arrow). Scale bar in (A) is for (A–E) . Wider field of this synaptic structure in serial sections were shown in (F–J) to see the surround structure. White arrow in (I, J) , a synaptic contact identifiable by classic methodology. The other synapse in the serial tangential sections also showed the similar progressive sequence (K–O) . (K) A large number of synaptic vesicles in presynaptic boutons (asterisk). (L) PSD traces at edges of the boutons with small vesicles (asterisk). The presynaptic boutons display some round-shaped electron dense substances (presynaptic grid-like objects). (M) Electron dense flocculate substance (black arrows, postsynaptic densities) in the postsynaptic spine (upper) and dendrite (lower). White arrow, a classically defined synapse contact. ( N ) Traces of the electron dense PSD in the cytoplasm of the postsynaptic spine (upper black arrow) and dendrite (lower black arrow). (O) Postsynaptic spine and dendrite without any synaptic objects. Scale bar in (F) is for (F–O) .
    Figure Legend Snippet: Serial ultrathin sections containing synapses with cleft plane cut in parallel or obliquely . (A–E) A synaptic contact with a cleft plane cut in parallel to the section plane is identified in the successive serial sections. In the first and second sections, many synaptic vesicles are found in the presynaptic bouton ( A, B , asterisk). The next section contains only a few vesicles and many round-shaped electron dense spots of about 35 nm diameter ( C , asterisk), that are likely to correspond to the pre-synaptic grid. The fourth section contains electron dense objects indicative of a PSD ( D , black arrow). In the fifth section, we observe a small part of the electron dense objects and the cytoplasm of the postsynaptic dendrite ( E , black arrow). Scale bar in (A) is for (A–E) . Wider field of this synaptic structure in serial sections were shown in (F–J) to see the surround structure. White arrow in (I, J) , a synaptic contact identifiable by classic methodology. The other synapse in the serial tangential sections also showed the similar progressive sequence (K–O) . (K) A large number of synaptic vesicles in presynaptic boutons (asterisk). (L) PSD traces at edges of the boutons with small vesicles (asterisk). The presynaptic boutons display some round-shaped electron dense substances (presynaptic grid-like objects). (M) Electron dense flocculate substance (black arrows, postsynaptic densities) in the postsynaptic spine (upper) and dendrite (lower). White arrow, a classically defined synapse contact. ( N ) Traces of the electron dense PSD in the cytoplasm of the postsynaptic spine (upper black arrow) and dendrite (lower black arrow). (O) Postsynaptic spine and dendrite without any synaptic objects. Scale bar in (F) is for (F–O) .

    Techniques Used: Sequencing

    The serial z-slice sections obtained by the tomography analysis showing the postsynaptic dendrite and spine in Figure 3 M . We picked up 16 z-slice sections (A–R) of good quality from the middle of the z-slice sequence. The estimated thickness of the z-slice section is 1.75 nm. The Intermediately electron dense flocculate substance presumably synaptic cleft structure [for example; asterisks in image ( C )] emerges continuously from the location where most synaptic boutons were found in the previous ultrathin section. Highly electron dense flocculate substance [for example; arrows in ( C, I, O )], PSD, emerged at the edge of the intermediate one [arrows in ( C )]. It gradually took over the domain of the intermediate electron dense flocculate substance ( A–R ).
    Figure Legend Snippet: The serial z-slice sections obtained by the tomography analysis showing the postsynaptic dendrite and spine in Figure 3 M . We picked up 16 z-slice sections (A–R) of good quality from the middle of the z-slice sequence. The estimated thickness of the z-slice section is 1.75 nm. The Intermediately electron dense flocculate substance presumably synaptic cleft structure [for example; asterisks in image ( C )] emerges continuously from the location where most synaptic boutons were found in the previous ultrathin section. Highly electron dense flocculate substance [for example; arrows in ( C, I, O )], PSD, emerged at the edge of the intermediate one [arrows in ( C )]. It gradually took over the domain of the intermediate electron dense flocculate substance ( A–R ).

    Techniques Used: Sequencing

    Thickness estimation of ultrathin sections for 3D reconstruction . (A) Minimal folds of an ultrathin section cut by a microtome set to cut 95 nm sections. Width of the minimal folds, twice the section thickness, was 130 nm, much less than expected. Note the black vertical line at the center of the fold, which is the adhered, folded membrane perpendicular to the plane of a section. (B) Correlation between section thicknesses set by the microtome and those measured by a color laser 3D microscope or the minimal folds method. The thicknesses measured by the optical method were more similar to those set by the microtome than those measured by the minimal folds method, which were 50–80% of the set section thickness. (C, D) Three-dimensional view of ultrathin section (90 nm thickness, pink) on glass slide (beige) obtained by the laser scanning microscope. The border of the section and slide surface is clearly identified in larger magnification (C) . (E) The pseudocolor image representing height of the ultrathin section (orange) and glass slide (blue). Note that the colors are uniformly distributed on the surface of the section and glass slide, indicating the surface flatness. Thickness of the ultrathin section was estimated from the difference between average heights of the two areas. Rectangles (about 50 μm 2 ), indicate areas used for evaluating the average height. (F) Light microscopic photograph of red blood cells. (G, H) The 3D images of the red blood cells reconstructed assuming 50 nm (G) and 90 nm (H) in section thickness. The view of the upper images is the same as in the light microscopic photograph in (F) ; the view of the lower images is “side-on”. The red blood cells reconstructed assuming a 90 nm section thickness were more similar in shape and size to those in the light microscopic photograph than were cells reconstructed assuming a 50 nm section thickness.
    Figure Legend Snippet: Thickness estimation of ultrathin sections for 3D reconstruction . (A) Minimal folds of an ultrathin section cut by a microtome set to cut 95 nm sections. Width of the minimal folds, twice the section thickness, was 130 nm, much less than expected. Note the black vertical line at the center of the fold, which is the adhered, folded membrane perpendicular to the plane of a section. (B) Correlation between section thicknesses set by the microtome and those measured by a color laser 3D microscope or the minimal folds method. The thicknesses measured by the optical method were more similar to those set by the microtome than those measured by the minimal folds method, which were 50–80% of the set section thickness. (C, D) Three-dimensional view of ultrathin section (90 nm thickness, pink) on glass slide (beige) obtained by the laser scanning microscope. The border of the section and slide surface is clearly identified in larger magnification (C) . (E) The pseudocolor image representing height of the ultrathin section (orange) and glass slide (blue). Note that the colors are uniformly distributed on the surface of the section and glass slide, indicating the surface flatness. Thickness of the ultrathin section was estimated from the difference between average heights of the two areas. Rectangles (about 50 μm 2 ), indicate areas used for evaluating the average height. (F) Light microscopic photograph of red blood cells. (G, H) The 3D images of the red blood cells reconstructed assuming 50 nm (G) and 90 nm (H) in section thickness. The view of the upper images is the same as in the light microscopic photograph in (F) ; the view of the lower images is “side-on”. The red blood cells reconstructed assuming a 90 nm section thickness were more similar in shape and size to those in the light microscopic photograph than were cells reconstructed assuming a 50 nm section thickness.

    Techniques Used: Microscopy, Laser-Scanning Microscopy

    16) Product Images from "Neocortical Inhibitory Terminals Innervate Dendritic Spines Targeted by Thalamocortical Afferents"

    Article Title: Neocortical Inhibitory Terminals Innervate Dendritic Spines Targeted by Thalamocortical Afferents

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.3846-06.2007

    Synaptic target structures for cortical nonpyramidal cells. A , Successive ultrathin sections showing a symmetrical synapse (white arrow) of the LS NG cell onto a postsynaptic soma. B , Successive ultrathin sections showing a symmetrical synapse (white arrow) from an RSNP DA-CRF cell onto a dendritic shaft with frequent asymmetrical inputs (black arrows). C , Successive ultrathin sections showing a symmetrical synapse (white arrow) from a BSNP DA-CR cell onto a spine head, which is also innervated by an asymmetrical synapse (black arrow). Scale bar in A also applies to B and C .
    Figure Legend Snippet: Synaptic target structures for cortical nonpyramidal cells. A , Successive ultrathin sections showing a symmetrical synapse (white arrow) of the LS NG cell onto a postsynaptic soma. B , Successive ultrathin sections showing a symmetrical synapse (white arrow) from an RSNP DA-CRF cell onto a dendritic shaft with frequent asymmetrical inputs (black arrows). C , Successive ultrathin sections showing a symmetrical synapse (white arrow) from a BSNP DA-CR cell onto a spine head, which is also innervated by an asymmetrical synapse (black arrow). Scale bar in A also applies to B and C .

    Techniques Used:

    Cortical spine innervated by a VGLUT2-positive terminal. A–H , Successive ultrathin sections of a spine head innervated by a VGLUT2-positive axon terminal (black arrow in F ). Two additional symmetrical synapses contacted this spine head (white arrows in F ).
    Figure Legend Snippet: Cortical spine innervated by a VGLUT2-positive terminal. A–H , Successive ultrathin sections of a spine head innervated by a VGLUT2-positive axon terminal (black arrow in F ). Two additional symmetrical synapses contacted this spine head (white arrows in F ).

    Techniques Used:

    Cortical spine innervated by a VGLUT1-positive terminal. A–N , Successive ultrathin sections of an entire spine head that was innervated by a VGLUT1-positive axon terminal. A single asymmetrical synapse was observed (arrow in F ).
    Figure Legend Snippet: Cortical spine innervated by a VGLUT1-positive terminal. A–N , Successive ultrathin sections of an entire spine head that was innervated by a VGLUT1-positive axon terminal. A single asymmetrical synapse was observed (arrow in F ).

    Techniques Used:

    17) Product Images from "Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development"

    Article Title: Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0046542

    Ultrathin sections of growing oocyte stained with RA47 (immunogold). a - mesogleal cell in adult medusa mesoglea; b – mesoglea embedded vegetative pole of the IV stage oocyte. 10 nm gold-conjugated secondary antibody used (Sigma, final dilution 1∶50). Symbols are the same as on fig. 4 and in addition: mc - mesogleal cell, Y – yolk granules, om – oocytes' basal membrane. White arrows indicate cistern-like structures in the lumen between oocyte and germinal epithelium. Letters with index (i.e. a′, c′ etc) indicate images of the high magnification i.e. parts from images with correspondent letters. Scale bar – 5 µm for a and a′, c and c′, d and e. Scale bar – 2 µm for b, d′ and d″, e′ and e″.
    Figure Legend Snippet: Ultrathin sections of growing oocyte stained with RA47 (immunogold). a - mesogleal cell in adult medusa mesoglea; b – mesoglea embedded vegetative pole of the IV stage oocyte. 10 nm gold-conjugated secondary antibody used (Sigma, final dilution 1∶50). Symbols are the same as on fig. 4 and in addition: mc - mesogleal cell, Y – yolk granules, om – oocytes' basal membrane. White arrows indicate cistern-like structures in the lumen between oocyte and germinal epithelium. Letters with index (i.e. a′, c′ etc) indicate images of the high magnification i.e. parts from images with correspondent letters. Scale bar – 5 µm for a and a′, c and c′, d and e. Scale bar – 2 µm for b, d′ and d″, e′ and e″.

    Techniques Used: Staining

    18) Product Images from "Dependence of GABAergic Synaptic Areas on the Interneuron Type and Target Size"

    Article Title: Dependence of GABAergic Synaptic Areas on the Interneuron Type and Target Size

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.20-01-00375.2000

    A, Relationship of the synaptic junction area to target structure circumference for three FS and three LTS cells. The data were obtained from the 3-D reconstructions of serial ultrathin sections. Synapses on dendritic shafts are represented by filled
    Figure Legend Snippet: A, Relationship of the synaptic junction area to target structure circumference for three FS and three LTS cells. The data were obtained from the 3-D reconstructions of serial ultrathin sections. Synapses on dendritic shafts are represented by filled

    Techniques Used:

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    Immunolabeling:

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    Article Snippet: .. Immunolabeling of ultrathin sections was carried out with the mouse monoclonal antibody to the HA epitope (diluted 1:500 in PBS containing 1% [w/v] acetylated BSA and 0.1% [v/v] Tween 20) and a goat anti-mouse IgG conjugated with 10 nm colloidal gold (Sigma). .. After immunolabeling, sections were poststained with uranyl acetate and lead citrate.

    Labeling:

    Article Title: Intracellular Localization of the Pseudorabies Virus Large Tegument Protein pUL36
    Article Snippet: .. The postembedding labeling of ultrathin sections was performed after blocking of surfaces with 1% cold water fish gelatin, 0.02 M glycine, and 1% bovine serum albumin fraction V (Sigma, Deisenhofen, Germany) in PBS, by either overnight incubation at 4°C or 2 h of incubation at room temperature with anti-pUL36 or anti-pUL31 antibodies diluted in PBS-bovine serum albumin. .. Diluted gold-tagged goat anti-species antibodies or protein A gold (GAR10 or PAG10 ; British BioCell, Int., Cambridge, United Kingdom) was added for 60 min at room temperature, and excess antibodies were removed by washing.

    Incubation:

    Article Title: Structural and molecular features of intestinal strictures in rats with Crohn's-like disease
    Article Snippet: .. Briefly, ultrathin sections from each block were sequentially incubated with anti-caspase 9 (Sigma-Aldrich, St. Louis, MO, United States; final dilution 1:50) primary antibodies overnight, followed by protein A-gold-conjugated anti-rabbit (18 nm gold particles, Jackson ImmunoResearch, West Grove, PA, United States; final dilution 1:20) secondary antibodies for 3 h, with extensive washing between. ..

    Article Title: A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure
    Article Snippet: .. The ultrathin sections were washed with 0.05 M TBS containing 0.1% Triton-X (TX) and incubated with rabbit antiserum against GABA (1:2500 or 1:5000; A-2052, Sigma-Aldrich, St. Louis, USA) in TBS containing 0.1% TX overnight. .. The ultrathin sections were then incubated with 15 nm colloidal gold conjugated anti-rabbit IgG (1:200; BBInternational #GAR15, Cardiff, UK) overnight in TBS containing 0.1% TX, and stained with 1% aqueous uranyl acetate followed by lead citrate.

    Article Title: The exon junction complex factor Y14 is dynamic in the nucleus of the beetle Tribolium castaneum during late oogenesis
    Article Snippet: .. Ultrathin sections were incubated for 10 min in blocking buffer containing 0.5% fish gelatin (Sigma) and 0.02% Tween-20 in PBS, pH 7.4. .. After blocking, the sections were incubated overnight in antibody 9E10 solution in a moist chamber at 4 °C.

    Article Title: Intracellular Localization of the Pseudorabies Virus Large Tegument Protein pUL36
    Article Snippet: .. The postembedding labeling of ultrathin sections was performed after blocking of surfaces with 1% cold water fish gelatin, 0.02 M glycine, and 1% bovine serum albumin fraction V (Sigma, Deisenhofen, Germany) in PBS, by either overnight incubation at 4°C or 2 h of incubation at room temperature with anti-pUL36 or anti-pUL31 antibodies diluted in PBS-bovine serum albumin. .. Diluted gold-tagged goat anti-species antibodies or protein A gold (GAR10 or PAG10 ; British BioCell, Int., Cambridge, United Kingdom) was added for 60 min at room temperature, and excess antibodies were removed by washing.

    Article Title: Expression of mesenchymal stem cell marker CD90 on dermal sheath cells of the anagen hair follicle in canine species
    Article Snippet: .. Ultrathin sections (90 nm) were subsequently cut and mounted on parlodion-coated 200-mesh nickel grids (Sigma), treated with 1% BSA in 0.1M TBS pH 7.4 for 5 min at room temperature and, then, incubated for 2 h at room temperature in a humid chamber with mouse monoclonal anti-CD90 antibody (VMRD) diluted 1:20 in a solution of 0.1 M TBS containing 1% BSA and 1% Normal Goat serum. .. After several washes in TBS to remove the excess antibody, the grids were incubated for 1 h at room temperature with goat antimouse antibody conjugated with 10 nm gold particles (Aurion) diluted 1:40 in 0.1M TBS pH 7.4 plus 1%BSA.

    Blocking Assay:

    Article Title: Structural and molecular features of intestinal strictures in rats with Crohn's-like disease
    Article Snippet: .. Briefly, ultrathin sections from each block were sequentially incubated with anti-caspase 9 (Sigma-Aldrich, St. Louis, MO, United States; final dilution 1:50) primary antibodies overnight, followed by protein A-gold-conjugated anti-rabbit (18 nm gold particles, Jackson ImmunoResearch, West Grove, PA, United States; final dilution 1:20) secondary antibodies for 3 h, with extensive washing between. ..

    Article Title: The exon junction complex factor Y14 is dynamic in the nucleus of the beetle Tribolium castaneum during late oogenesis
    Article Snippet: .. Ultrathin sections were incubated for 10 min in blocking buffer containing 0.5% fish gelatin (Sigma) and 0.02% Tween-20 in PBS, pH 7.4. .. After blocking, the sections were incubated overnight in antibody 9E10 solution in a moist chamber at 4 °C.

    Article Title: Intracellular Localization of the Pseudorabies Virus Large Tegument Protein pUL36
    Article Snippet: .. The postembedding labeling of ultrathin sections was performed after blocking of surfaces with 1% cold water fish gelatin, 0.02 M glycine, and 1% bovine serum albumin fraction V (Sigma, Deisenhofen, Germany) in PBS, by either overnight incubation at 4°C or 2 h of incubation at room temperature with anti-pUL36 or anti-pUL31 antibodies diluted in PBS-bovine serum albumin. .. Diluted gold-tagged goat anti-species antibodies or protein A gold (GAR10 or PAG10 ; British BioCell, Int., Cambridge, United Kingdom) was added for 60 min at room temperature, and excess antibodies were removed by washing.

    Fluorescence In Situ Hybridization:

    Article Title: The exon junction complex factor Y14 is dynamic in the nucleus of the beetle Tribolium castaneum during late oogenesis
    Article Snippet: .. Ultrathin sections were incubated for 10 min in blocking buffer containing 0.5% fish gelatin (Sigma) and 0.02% Tween-20 in PBS, pH 7.4. .. After blocking, the sections were incubated overnight in antibody 9E10 solution in a moist chamber at 4 °C.

    Article Title: Intracellular Localization of the Pseudorabies Virus Large Tegument Protein pUL36
    Article Snippet: .. The postembedding labeling of ultrathin sections was performed after blocking of surfaces with 1% cold water fish gelatin, 0.02 M glycine, and 1% bovine serum albumin fraction V (Sigma, Deisenhofen, Germany) in PBS, by either overnight incubation at 4°C or 2 h of incubation at room temperature with anti-pUL36 or anti-pUL31 antibodies diluted in PBS-bovine serum albumin. .. Diluted gold-tagged goat anti-species antibodies or protein A gold (GAR10 or PAG10 ; British BioCell, Int., Cambridge, United Kingdom) was added for 60 min at room temperature, and excess antibodies were removed by washing.

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    Millipore serial ultrathin sections
    Validation of the synaptic labeling tool. (A) A representative image showing functional presynaptic terminals labeled by FM dye staining (blue) in 14 DIV (days in vitro) dissociated primary hippocampal neurons generated from a P0 triple transgenic mouse pup (ZtTA/wt; TRE-Bi-SG-T/wt; Nestin-Cre/wt). The presynaptic termini, labeled by Syp-GFP (green) and the FM dye (blue) are located on axonal processes (red) and therefore appear white or yellowish. (B) The Syp-GFP and FM-dye channels from panel A have been shifted to show that most Syp-GFP puncta (green, white arrowheads) located along the processes colocalize with FM dye (blue) puncta. Scale bar is 5 µm. (C) Quantification of percentage of Syp-GFP colocalized with FM dye (Grey bar for FM dye experiment in A–B), synapsin and MAGUK (for array tomography experiment in D–E). The total number of counted Syp-GFP puncta is 1176 and 314, for the FM dye experiment and array tomography experiment, respectively. Two animals were used for the FM dye experiment. (D–E) Representative array tomography images from a P35 triple transgenic mouse brain (ZtTA/wt; TRE-Bi-SG-T/wt; β-actin-CreER/wt) with tamoxifen administered at E9.5 showing in vivo presynaptic localization of Syp-GFP puncta. Left panels represent images from a single <t>ultrathin</t> (70 nm) section showing colocalization of Syp-GFP (green) with synapsin (red, C) or MAGUK (red, D). Right panels show two examples (each example in a single column) of 4 serial sections through a single Syp-GFP punctum.
    Serial Ultrathin Sections, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Validation of the synaptic labeling tool. (A) A representative image showing functional presynaptic terminals labeled by FM dye staining (blue) in 14 DIV (days in vitro) dissociated primary hippocampal neurons generated from a P0 triple transgenic mouse pup (ZtTA/wt; TRE-Bi-SG-T/wt; Nestin-Cre/wt). The presynaptic termini, labeled by Syp-GFP (green) and the FM dye (blue) are located on axonal processes (red) and therefore appear white or yellowish. (B) The Syp-GFP and FM-dye channels from panel A have been shifted to show that most Syp-GFP puncta (green, white arrowheads) located along the processes colocalize with FM dye (blue) puncta. Scale bar is 5 µm. (C) Quantification of percentage of Syp-GFP colocalized with FM dye (Grey bar for FM dye experiment in A–B), synapsin and MAGUK (for array tomography experiment in D–E). The total number of counted Syp-GFP puncta is 1176 and 314, for the FM dye experiment and array tomography experiment, respectively. Two animals were used for the FM dye experiment. (D–E) Representative array tomography images from a P35 triple transgenic mouse brain (ZtTA/wt; TRE-Bi-SG-T/wt; β-actin-CreER/wt) with tamoxifen administered at E9.5 showing in vivo presynaptic localization of Syp-GFP puncta. Left panels represent images from a single ultrathin (70 nm) section showing colocalization of Syp-GFP (green) with synapsin (red, C) or MAGUK (red, D). Right panels show two examples (each example in a single column) of 4 serial sections through a single Syp-GFP punctum.

    Journal: PLoS ONE

    Article Title: Visualizing the Distribution of Synapses from Individual Neurons in the Mouse Brain

    doi: 10.1371/journal.pone.0011503

    Figure Lengend Snippet: Validation of the synaptic labeling tool. (A) A representative image showing functional presynaptic terminals labeled by FM dye staining (blue) in 14 DIV (days in vitro) dissociated primary hippocampal neurons generated from a P0 triple transgenic mouse pup (ZtTA/wt; TRE-Bi-SG-T/wt; Nestin-Cre/wt). The presynaptic termini, labeled by Syp-GFP (green) and the FM dye (blue) are located on axonal processes (red) and therefore appear white or yellowish. (B) The Syp-GFP and FM-dye channels from panel A have been shifted to show that most Syp-GFP puncta (green, white arrowheads) located along the processes colocalize with FM dye (blue) puncta. Scale bar is 5 µm. (C) Quantification of percentage of Syp-GFP colocalized with FM dye (Grey bar for FM dye experiment in A–B), synapsin and MAGUK (for array tomography experiment in D–E). The total number of counted Syp-GFP puncta is 1176 and 314, for the FM dye experiment and array tomography experiment, respectively. Two animals were used for the FM dye experiment. (D–E) Representative array tomography images from a P35 triple transgenic mouse brain (ZtTA/wt; TRE-Bi-SG-T/wt; β-actin-CreER/wt) with tamoxifen administered at E9.5 showing in vivo presynaptic localization of Syp-GFP puncta. Left panels represent images from a single ultrathin (70 nm) section showing colocalization of Syp-GFP (green) with synapsin (red, C) or MAGUK (red, D). Right panels show two examples (each example in a single column) of 4 serial sections through a single Syp-GFP punctum.

    Article Snippet: Serial ultrathin sections (70 nm) were cut on an ultramicrotome (Leica), mounted on coverslips and immunostained with antibodies against Synapsin I (rabbit, Millipore AB1543P, 1∶100) and MAGUK (mouse, NeuroMabs, 75-029, 1∶100).

    Techniques: Labeling, Functional Assay, Staining, In Vitro, Generated, Transgenic Assay, In Vivo

    High-resolution array tomographic images rendered from 16 ultrathin (70-nm) sections. ( A ) A field of view showing the high density of TPOH-labeled dendrites and soma (white), abundantly surrounded by synapsin (blue) and GAD2 (red) puncta. ( B ) Closer view

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Presynaptic gating of excitation in the dorsal raphe nucleus by GABA

    doi: 10.1073/pnas.1304505110

    Figure Lengend Snippet: High-resolution array tomographic images rendered from 16 ultrathin (70-nm) sections. ( A ) A field of view showing the high density of TPOH-labeled dendrites and soma (white), abundantly surrounded by synapsin (blue) and GAD2 (red) puncta. ( B ) Closer view

    Article Snippet: Serial ultrathin sections were immunolabeled as described ( , ) using primary antisera raised against TPOH (sheep, 1:200; Millipore; AB1541) and VGLUT1, VGLUT2, and VGLUT3 (all three guinea pig, 1:1,000; Millipore; AB5905, AB2251, and AB5421, respectively) and monoclonal antibodies for synapsin 1 and GAD2 (rabbit, 1:200; Cell Signaling Technologies; 5297 and 5843, respectively).

    Techniques: Labeling