Structured Review

Merck KGaA ultrathin sections
Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
Ultrathin Sections, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ultrathin sections/product/Merck KGaA
Average 93 stars, based on 29 article reviews
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ultrathin sections - by Bioz Stars, 2020-08
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1) Product Images from "Microscopic and ultrastructural evidences in human skin following calcium hydroxylapatite filler treatment"

Article Title: Microscopic and ultrastructural evidences in human skin following calcium hydroxylapatite filler treatment

Journal: Archives of Dermatological Research

doi: 10.1007/s00403-017-1734-3

Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making ultrathin sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
Figure Legend Snippet: Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making ultrathin sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable

Techniques Used: Electron Microscopy

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Staining:

Article Title: An ultrastructural study of cell death in the CA1 pyramidal field of the hippocapmus in rats submitted to transient global ischemia followed by reperfusion
Article Snippet: .. Then ultrathin sections (80 nm) were obtained with the same ultramicrotome and stained with 2% lead citrate (Merck, Darmstadt, Germany) and 1% uranyl acetate (Merck) , and then examined using a transmission electron microscope (JEM 1200 EXII, Akashima, Japan, CME-UFRGS). ..

Immunolabeling:

Article Title: Impact of Astrocyte Depletion upon Inflammation and Demyelination in a Murine Animal Model of Multiple Sclerosis
Article Snippet: .. Ultrathin sections were prepared and immunolabeled with an anti-AQP4 antibody (1:50 dilution; Merck Millipore, Danvers, Massachusetts, USA), followed by a goat anti-Rabbit IgG 10nm Immunogold conjugate secondary antibody (BBI Solutions, Crumlin, United Kingdom). .. The sections were contrasted with uranyl acetate and lead citrate and then evaluated on a transmission electron microscope (EM 10A, Carl Zeiss Microscopy GmbH, Jena, Germany).

Microscopy:

Article Title: Evaluation of zebrafish as a model to study the pathogenesis of the opportunistic pathogen Cronobacter turicensis
Article Snippet: .. Ultrathin sections (80 nm) were mounted on copper grids (Merck Eurolab AG, Dietlikon, Switzerland), contrasted with uranyl acetate dihydrate (Sigma-Aldrich, Buchs, Switzerland) and lead citrate (Merck Eurolab AG, Dietlikon, Switzerland) and investigated using a Philips CM10 transmission electron microscope (Philips Electron Optics, Eindhoven, The Netherlands). .. Images were processed with Imaris (Bitplane AG, Zurich, Switzerland) and assembled for publication using Adobe Photoshop.

Article Title: An ultrastructural study of cell death in the CA1 pyramidal field of the hippocapmus in rats submitted to transient global ischemia followed by reperfusion
Article Snippet: .. Then ultrathin sections (80 nm) were obtained with the same ultramicrotome and stained with 2% lead citrate (Merck, Darmstadt, Germany) and 1% uranyl acetate (Merck) , and then examined using a transmission electron microscope (JEM 1200 EXII, Akashima, Japan, CME-UFRGS). ..

Article Title: Mixed infections in vitro with different Chlamydiaceae strains and a cell culture adapted porcine epidemic diarrhea virus
Article Snippet: .. Ultrathin sections (80 nm) were mounted on copper grids (Merck Eurolab AG, Dietlikon, Switzerland), contrasted with uranyl acetate dihydrate (Fluka) and lead citrate (lead nitrate and tri-natrium dihydrate; Merck Eurolab AG) and investigated in an electron microscope (Philips CM10). .. 2.8 Statistical analysis To determine whether numbers of chlamydial inclusions or ca-PEDV syncytia differed between mono- and dually infected cell layers, the chi-square test and the Wilcoxon signed-rank test were used.

Transmission Assay:

Article Title: Evaluation of zebrafish as a model to study the pathogenesis of the opportunistic pathogen Cronobacter turicensis
Article Snippet: .. Ultrathin sections (80 nm) were mounted on copper grids (Merck Eurolab AG, Dietlikon, Switzerland), contrasted with uranyl acetate dihydrate (Sigma-Aldrich, Buchs, Switzerland) and lead citrate (Merck Eurolab AG, Dietlikon, Switzerland) and investigated using a Philips CM10 transmission electron microscope (Philips Electron Optics, Eindhoven, The Netherlands). .. Images were processed with Imaris (Bitplane AG, Zurich, Switzerland) and assembled for publication using Adobe Photoshop.

Article Title: An ultrastructural study of cell death in the CA1 pyramidal field of the hippocapmus in rats submitted to transient global ischemia followed by reperfusion
Article Snippet: .. Then ultrathin sections (80 nm) were obtained with the same ultramicrotome and stained with 2% lead citrate (Merck, Darmstadt, Germany) and 1% uranyl acetate (Merck) , and then examined using a transmission electron microscope (JEM 1200 EXII, Akashima, Japan, CME-UFRGS). ..

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    Merck KGaA ultrathin sections
    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making <t>ultrathin</t> sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable
    Ultrathin Sections, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/Merck KGaA
    Average 93 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making ultrathin sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable

    Journal: Archives of Dermatological Research

    Article Title: Microscopic and ultrastructural evidences in human skin following calcium hydroxylapatite filler treatment

    doi: 10.1007/s00403-017-1734-3

    Figure Lengend Snippet: Electron microscopy. a Part of a microspherule inside the deep dermis, well identifiable in the half lower part of the electron micrograph, due to the very high electron density of microgranules related to the calcium hydroxylapatite content. The upper part of the figure is constituted by compact bundles of collagen fibres ( C ) transversally or obliquely sectioned. Inside them, some microgranules can be recognized due to their high electron density. Inset high magnification of a microspherule showing compacted microgranules ( dark ). The grey material between microgranules is representing the gel carrier. The white small areas associated with microgranules are representing artefacts due to the ripping of the very hard microgranules on the diamond knife making ultrathin sections at the ultramicrotome. b Big cell with a nucleus rich of euchromatin ( N ) showing also a well-visible nucleolus ( arrow ), in close relationship with microgranules, free into the extracellular matrix ( left ) and at the surface of a microspherule ( high right and low left ). The cytoplasm of the cell is extremely rich of vesicles with a clear and homogeneous content. No intracytoplasmic microgranules or phagocytotic structures are observable

    Article Snippet: Ultrathin sections, after the collection on 200 mesh grids, were counterstained with lead citrate and uranyl acetate (Merck, Darmstadt, Germany).

    Techniques: Electron Microscopy