Structured Review

Epitomics ultrathin sections
The nuclear membrane vesiculates after activation. <t>Ultrathin</t> cryosections of neutrophils stimulated with 20 nM PMA for 60 (a) and 180 (d) min and labeled with an antibody against the nuclear envelope–specific lamin B receptor. Bound antibody was detected with a gold-conjugated secondary antibody. In 60-min–stimulated neutrophils, the lamin B receptor was found on the inner nuclear membrane, as expected (a, arrows). 180 min after stimulation, the lamin B receptor was found in vesicles (d, arrows) in the cytoplasm. Nuclear material (d, arrowheads) is decondensed and not enclosed by a membrane. (b) 60 min after activation, neutrophils have flattened out and exhibit a lobulated nucleus and numerous granules. (c) Immunofluorescence staining for the nuclear membrane delineates the nuclear lobules (green) and is in continuity with the endoplasmic reticulum (red). (e and f) After 180 min of stimulation, neutrophil nuclei are inflated and fill nearly the entire cell. Some remaining granules are found in the periphery of the cell, while remnants of the nuclear membrane and the endoplasmic reticulum are restricted to a small central area surrounded by nuclear material (arrows). Bars: (a and d) 500 nm; (b, c, e, and d) 10 μm.
Ultrathin Sections, supplied by Epitomics, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Novel cell death program leads to neutrophil extracellular traps

Journal: The Journal of Cell Biology

doi: 10.1083/jcb.200606027

The nuclear membrane vesiculates after activation. Ultrathin cryosections of neutrophils stimulated with 20 nM PMA for 60 (a) and 180 (d) min and labeled with an antibody against the nuclear envelope–specific lamin B receptor. Bound antibody was detected with a gold-conjugated secondary antibody. In 60-min–stimulated neutrophils, the lamin B receptor was found on the inner nuclear membrane, as expected (a, arrows). 180 min after stimulation, the lamin B receptor was found in vesicles (d, arrows) in the cytoplasm. Nuclear material (d, arrowheads) is decondensed and not enclosed by a membrane. (b) 60 min after activation, neutrophils have flattened out and exhibit a lobulated nucleus and numerous granules. (c) Immunofluorescence staining for the nuclear membrane delineates the nuclear lobules (green) and is in continuity with the endoplasmic reticulum (red). (e and f) After 180 min of stimulation, neutrophil nuclei are inflated and fill nearly the entire cell. Some remaining granules are found in the periphery of the cell, while remnants of the nuclear membrane and the endoplasmic reticulum are restricted to a small central area surrounded by nuclear material (arrows). Bars: (a and d) 500 nm; (b, c, e, and d) 10 μm.
Figure Legend Snippet: The nuclear membrane vesiculates after activation. Ultrathin cryosections of neutrophils stimulated with 20 nM PMA for 60 (a) and 180 (d) min and labeled with an antibody against the nuclear envelope–specific lamin B receptor. Bound antibody was detected with a gold-conjugated secondary antibody. In 60-min–stimulated neutrophils, the lamin B receptor was found on the inner nuclear membrane, as expected (a, arrows). 180 min after stimulation, the lamin B receptor was found in vesicles (d, arrows) in the cytoplasm. Nuclear material (d, arrowheads) is decondensed and not enclosed by a membrane. (b) 60 min after activation, neutrophils have flattened out and exhibit a lobulated nucleus and numerous granules. (c) Immunofluorescence staining for the nuclear membrane delineates the nuclear lobules (green) and is in continuity with the endoplasmic reticulum (red). (e and f) After 180 min of stimulation, neutrophil nuclei are inflated and fill nearly the entire cell. Some remaining granules are found in the periphery of the cell, while remnants of the nuclear membrane and the endoplasmic reticulum are restricted to a small central area surrounded by nuclear material (arrows). Bars: (a and d) 500 nm; (b, c, e, and d) 10 μm.

Techniques Used: Activation Assay, Labeling, Immunofluorescence, Staining

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    Epitomics ultrathin sections
    The nuclear membrane vesiculates after activation. <t>Ultrathin</t> cryosections of neutrophils stimulated with 20 nM PMA for 60 (a) and 180 (d) min and labeled with an antibody against the nuclear envelope–specific lamin B receptor. Bound antibody was detected with a gold-conjugated secondary antibody. In 60-min–stimulated neutrophils, the lamin B receptor was found on the inner nuclear membrane, as expected (a, arrows). 180 min after stimulation, the lamin B receptor was found in vesicles (d, arrows) in the cytoplasm. Nuclear material (d, arrowheads) is decondensed and not enclosed by a membrane. (b) 60 min after activation, neutrophils have flattened out and exhibit a lobulated nucleus and numerous granules. (c) Immunofluorescence staining for the nuclear membrane delineates the nuclear lobules (green) and is in continuity with the endoplasmic reticulum (red). (e and f) After 180 min of stimulation, neutrophil nuclei are inflated and fill nearly the entire cell. Some remaining granules are found in the periphery of the cell, while remnants of the nuclear membrane and the endoplasmic reticulum are restricted to a small central area surrounded by nuclear material (arrows). Bars: (a and d) 500 nm; (b, c, e, and d) 10 μm.
    Ultrathin Sections, supplied by Epitomics, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/Epitomics
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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    The nuclear membrane vesiculates after activation. Ultrathin cryosections of neutrophils stimulated with 20 nM PMA for 60 (a) and 180 (d) min and labeled with an antibody against the nuclear envelope–specific lamin B receptor. Bound antibody was detected with a gold-conjugated secondary antibody. In 60-min–stimulated neutrophils, the lamin B receptor was found on the inner nuclear membrane, as expected (a, arrows). 180 min after stimulation, the lamin B receptor was found in vesicles (d, arrows) in the cytoplasm. Nuclear material (d, arrowheads) is decondensed and not enclosed by a membrane. (b) 60 min after activation, neutrophils have flattened out and exhibit a lobulated nucleus and numerous granules. (c) Immunofluorescence staining for the nuclear membrane delineates the nuclear lobules (green) and is in continuity with the endoplasmic reticulum (red). (e and f) After 180 min of stimulation, neutrophil nuclei are inflated and fill nearly the entire cell. Some remaining granules are found in the periphery of the cell, while remnants of the nuclear membrane and the endoplasmic reticulum are restricted to a small central area surrounded by nuclear material (arrows). Bars: (a and d) 500 nm; (b, c, e, and d) 10 μm.

    Journal: The Journal of Cell Biology

    Article Title: Novel cell death program leads to neutrophil extracellular traps

    doi: 10.1083/jcb.200606027

    Figure Lengend Snippet: The nuclear membrane vesiculates after activation. Ultrathin cryosections of neutrophils stimulated with 20 nM PMA for 60 (a) and 180 (d) min and labeled with an antibody against the nuclear envelope–specific lamin B receptor. Bound antibody was detected with a gold-conjugated secondary antibody. In 60-min–stimulated neutrophils, the lamin B receptor was found on the inner nuclear membrane, as expected (a, arrows). 180 min after stimulation, the lamin B receptor was found in vesicles (d, arrows) in the cytoplasm. Nuclear material (d, arrowheads) is decondensed and not enclosed by a membrane. (b) 60 min after activation, neutrophils have flattened out and exhibit a lobulated nucleus and numerous granules. (c) Immunofluorescence staining for the nuclear membrane delineates the nuclear lobules (green) and is in continuity with the endoplasmic reticulum (red). (e and f) After 180 min of stimulation, neutrophil nuclei are inflated and fill nearly the entire cell. Some remaining granules are found in the periphery of the cell, while remnants of the nuclear membrane and the endoplasmic reticulum are restricted to a small central area surrounded by nuclear material (arrows). Bars: (a and d) 500 nm; (b, c, e, and d) 10 μm.

    Article Snippet: Ultrathin sections were cut at −105°C, blocked, and reacted with primary antibodies (anti-LBR; Epitomics), followed by secondary antibodies coupled to 6- or 12-nm gold particles.

    Techniques: Activation Assay, Labeling, Immunofluorescence, Staining