Structured Review

Carl Zeiss ultrathin sections
Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) <t>Ultrathin</t> section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
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1) Product Images from "The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication"

Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

Journal: PLoS ONE

doi: 10.1371/journal.pone.0083386

Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) Ultrathin section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
Figure Legend Snippet: Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) Ultrathin section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

Techniques Used: Staining

Histopathological and ultrastructural analysis of the lung. (a) Lung of a non-dengue case stained with HE and presenting normal aspect of alveoli (A) and alveolar septa (AS). (b–g) Lung sections of dengue cases, stained with HE, showing pulmonary alterations, including septal thickening (St), edema (E), hemorrhage (He), presence of mononuclear infiltrate (Inf), hyaline membrane formation (HM) and hypertrophy of alveolar macrophages (AM) and type II pneumocytes (PcyII). (h) Semi-thin section of a non-dengue case showing alveoli (A), alveolar septa (AS), endothelial cells (EC) and type I (PcyI) and II pneumocytes (PcyII) with normal aspects. (i) Semi-thin section of one dengue case presenting numerous platelets (Pt) and megakaryocytes (MK) inside alveolar septa. (j) Ultrathin section of one non-dengue case exhibiting regular alveoli, alveolar septum, type I and II pneumocytes and endothelial cell. (j-l) Ultrathin sections of dengue cases exhibited type II pneumocytes located in alveolar space in contact with numerous platelets, the appearance of hyaline membrane and the presence of virus particles (VP) in endothelium. Quantitative analysis of hemorrhage (m) and edema (n) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
Figure Legend Snippet: Histopathological and ultrastructural analysis of the lung. (a) Lung of a non-dengue case stained with HE and presenting normal aspect of alveoli (A) and alveolar septa (AS). (b–g) Lung sections of dengue cases, stained with HE, showing pulmonary alterations, including septal thickening (St), edema (E), hemorrhage (He), presence of mononuclear infiltrate (Inf), hyaline membrane formation (HM) and hypertrophy of alveolar macrophages (AM) and type II pneumocytes (PcyII). (h) Semi-thin section of a non-dengue case showing alveoli (A), alveolar septa (AS), endothelial cells (EC) and type I (PcyI) and II pneumocytes (PcyII) with normal aspects. (i) Semi-thin section of one dengue case presenting numerous platelets (Pt) and megakaryocytes (MK) inside alveolar septa. (j) Ultrathin section of one non-dengue case exhibiting regular alveoli, alveolar septum, type I and II pneumocytes and endothelial cell. (j-l) Ultrathin sections of dengue cases exhibited type II pneumocytes located in alveolar space in contact with numerous platelets, the appearance of hyaline membrane and the presence of virus particles (VP) in endothelium. Quantitative analysis of hemorrhage (m) and edema (n) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

Techniques Used: Staining

Histopathological and ultrastructural analysis of the spleen. (a) Spleen of a non-dengue case stained with HE and presenting normal aspect. (b and c) Spleen sections of dengue cases, stained with HE, showing vascular congestion (VC), edema (E) and an atrophy of lymphoid follicles. Red pulp (RP); white pulp (WP). (d) Semi-thin section of a non-dengue case revealing red pulp with regular aspect and normal splenocytes (S). (e) Semi-thin section of a dengue case showing vacuolization (V) and degenerated splenocytes (DS). (f) Ultra-thin section of a non-dengue case with regular splenocytes (S) and (g and h) dengue cases exhibiting vacuolization (V) around degenerated splenocytes and loss of the endothelium of splenic sinusoid (SS). Semi-thin and ultrathin sections were stained as described in figure 1 . (i–k) Quantitative analysis of histological damages observed individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). The media of lymphoid follicle areas were quantified (i), as well as the percentage areas with vascular congestion (j) and edema (k). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
Figure Legend Snippet: Histopathological and ultrastructural analysis of the spleen. (a) Spleen of a non-dengue case stained with HE and presenting normal aspect. (b and c) Spleen sections of dengue cases, stained with HE, showing vascular congestion (VC), edema (E) and an atrophy of lymphoid follicles. Red pulp (RP); white pulp (WP). (d) Semi-thin section of a non-dengue case revealing red pulp with regular aspect and normal splenocytes (S). (e) Semi-thin section of a dengue case showing vacuolization (V) and degenerated splenocytes (DS). (f) Ultra-thin section of a non-dengue case with regular splenocytes (S) and (g and h) dengue cases exhibiting vacuolization (V) around degenerated splenocytes and loss of the endothelium of splenic sinusoid (SS). Semi-thin and ultrathin sections were stained as described in figure 1 . (i–k) Quantitative analysis of histological damages observed individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). The media of lymphoid follicle areas were quantified (i), as well as the percentage areas with vascular congestion (j) and edema (k). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

Techniques Used: Staining

Histopathological and ultrastructural analysis of the heart. (a) Heart of a non-dengue case stained with H.E. and presenting normal aspect. (b and c) Heart sections of dengue cases, stained with HE, showing cardiac injuries, including hemorrhage (He), edema (E), presence of mononuclear infiltrate (Inf) and degeneration of muscle fibers (black star). (d and f) Semi-thin and ultrathin sections of a non-dengue case presenting cardiac fibers (CF) with normal nucleus (N), mitochondria (M), capillaries (Cap) and intercalated discs (ID). (e) Semi-thin section of one dengue case presenting degeneration of cardiac fibers (black star) characterized by absence of nucleus and a diffuse interstitial edema (E) and (g and h) ultrathin sections showing nuclear (white stars) and mitochondria alterations (M) in cardiomyocytes and interstitial edema. Quantitative analysis of hemorrhage (i) and edema (j) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
Figure Legend Snippet: Histopathological and ultrastructural analysis of the heart. (a) Heart of a non-dengue case stained with H.E. and presenting normal aspect. (b and c) Heart sections of dengue cases, stained with HE, showing cardiac injuries, including hemorrhage (He), edema (E), presence of mononuclear infiltrate (Inf) and degeneration of muscle fibers (black star). (d and f) Semi-thin and ultrathin sections of a non-dengue case presenting cardiac fibers (CF) with normal nucleus (N), mitochondria (M), capillaries (Cap) and intercalated discs (ID). (e) Semi-thin section of one dengue case presenting degeneration of cardiac fibers (black star) characterized by absence of nucleus and a diffuse interstitial edema (E) and (g and h) ultrathin sections showing nuclear (white stars) and mitochondria alterations (M) in cardiomyocytes and interstitial edema. Quantitative analysis of hemorrhage (i) and edema (j) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

Techniques Used: Staining

Histopathological/ultrastructural analysis and dengue detection in the kidney. (a) Kidney of a non-dengue case stained with HE and presenting normal aspect. (b and c) Kidney sections of dengue cases, stained with HE, showing injuries, including: hemorrhage (He), edema (E), sloughing of necrotic cells with loss of the basement membrane (black star), mainly in proximal convoluted tubule (PCT) but also detected in distal convoluted tubule (DCT), and areas of cellular regeneration (blue star) near renal glomerulus (RG). (d and e) Semi-thin sections of non-dengue cases showing Bowman’s capsule (BC) and podocytes (Pdc) around glomerular capillaries (GC), mensagial cells (MC) and endothelial cell (EC) with regular structures and preserved capillaries (Cap), epithelial cells (Ep) and distal and proximal convolutes tubules (DCT and PCT, respectively). (f and g) Dengue cases with the presence of thrombus (T) in capillaries of renal glomerulus and necrotic cells (NC) in the lumen of proximal convoluted tubules. (h) Ultrathin of a non-dengue case exhibiting conserved glomerular capillaries. (i) Ultrathin of one dengue case showing necrotic cell with picnotic nucleus (white star) and dilatation of rough endoplasmic reticulum (ER). Quantitative analysis of hemorrhage (j) and edema (k) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
Figure Legend Snippet: Histopathological/ultrastructural analysis and dengue detection in the kidney. (a) Kidney of a non-dengue case stained with HE and presenting normal aspect. (b and c) Kidney sections of dengue cases, stained with HE, showing injuries, including: hemorrhage (He), edema (E), sloughing of necrotic cells with loss of the basement membrane (black star), mainly in proximal convoluted tubule (PCT) but also detected in distal convoluted tubule (DCT), and areas of cellular regeneration (blue star) near renal glomerulus (RG). (d and e) Semi-thin sections of non-dengue cases showing Bowman’s capsule (BC) and podocytes (Pdc) around glomerular capillaries (GC), mensagial cells (MC) and endothelial cell (EC) with regular structures and preserved capillaries (Cap), epithelial cells (Ep) and distal and proximal convolutes tubules (DCT and PCT, respectively). (f and g) Dengue cases with the presence of thrombus (T) in capillaries of renal glomerulus and necrotic cells (NC) in the lumen of proximal convoluted tubules. (h) Ultrathin of a non-dengue case exhibiting conserved glomerular capillaries. (i) Ultrathin of one dengue case showing necrotic cell with picnotic nucleus (white star) and dilatation of rough endoplasmic reticulum (ER). Quantitative analysis of hemorrhage (j) and edema (k) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

Techniques Used: Staining

2) Product Images from "Fusion of Hematopoietic Cells with Purkinje Neurons Does Not Lead to Stable Heterokaryon Formation under Noninvasive Conditions"

Article Title: Fusion of Hematopoietic Cells with Purkinje Neurons Does Not Lead to Stable Heterokaryon Formation under Noninvasive Conditions

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.5848-08.2009

Electron microscopic confirmation of single nuclei in LacZ-labeled Purkinje neurons in vav-iCre/LacZ mice. a , Example of a LacZ-positive Purkinje neuron, detected by X-gal staining of a 50-μm-thick Vibratome section, located in the Purkinje neuron layer (PC), between molecular (mol) and granule (gr) cell layer. b , Larger image of the same cell as in a . c , Light microscopic staining of calbindin confirming that all large X-gal-positive cell bodies in the PC are indeed Purkinje neurons. Typical dotted X-gal stain in the cell body and dendrite indicated by black arrowheads. d , e , Serial ultrathin sections through the same PKN as shown in a and b reveal only a single nucleus. The nucleus is large and light with a dark basophilic nucleolus, with deep folds and invaginations toward the side of the dendrites and Nissl bodies (NB) at the side of the folds, thus displaying the typical morphologic features of Purkinje neurons. f , g , Same cell at different z -levels and smaller magnification showing no second nucleus in this neuron. Scale bars: a–c , 20 μm; d , e , 2 μm; f , g , 5 μm.
Figure Legend Snippet: Electron microscopic confirmation of single nuclei in LacZ-labeled Purkinje neurons in vav-iCre/LacZ mice. a , Example of a LacZ-positive Purkinje neuron, detected by X-gal staining of a 50-μm-thick Vibratome section, located in the Purkinje neuron layer (PC), between molecular (mol) and granule (gr) cell layer. b , Larger image of the same cell as in a . c , Light microscopic staining of calbindin confirming that all large X-gal-positive cell bodies in the PC are indeed Purkinje neurons. Typical dotted X-gal stain in the cell body and dendrite indicated by black arrowheads. d , e , Serial ultrathin sections through the same PKN as shown in a and b reveal only a single nucleus. The nucleus is large and light with a dark basophilic nucleolus, with deep folds and invaginations toward the side of the dendrites and Nissl bodies (NB) at the side of the folds, thus displaying the typical morphologic features of Purkinje neurons. f , g , Same cell at different z -levels and smaller magnification showing no second nucleus in this neuron. Scale bars: a–c , 20 μm; d , e , 2 μm; f , g , 5 μm.

Techniques Used: Labeling, Mouse Assay, Staining

3) Product Images from "Molecular mechanisms in uterine epithelium during trophoblast binding: the role of small GTPase RhoA in human uterine Ishikawa cells"

Article Title: Molecular mechanisms in uterine epithelium during trophoblast binding: the role of small GTPase RhoA in human uterine Ishikawa cells

Journal: Journal of Experimental & Clinical Assisted Reproduction

doi: 10.1186/1743-1050-2-4

Ultrathin sections of Ishikawa cells. A : Ishikawa cells before treatment with toxin A. Cells grow as monolayers and show apico-basal polarity with nuclei located at the base of the cells and organelles found predominantly in the supranuclear region of the cells. Insert (light microscopy, cross section) shows overview of Ishikawa cells growing as monolayers. B : Lateral cell membranes show tight junctions, adherens junctions and desmosomes in varying combinations, whereas regular junctional complexes consisting of tight junction, adherens junction and desmosomes in apico-basal sequence were rarely seen. C : Ishikawa monolayers after treatment with toxin A. Standard electron microscopy showed no substantial differences between untreated (A) and toxin A-treated cells (C). Me: cell culture medium; N: nucleus; oo: coverslip; *: desmosome; arrow: adherens junction.
Figure Legend Snippet: Ultrathin sections of Ishikawa cells. A : Ishikawa cells before treatment with toxin A. Cells grow as monolayers and show apico-basal polarity with nuclei located at the base of the cells and organelles found predominantly in the supranuclear region of the cells. Insert (light microscopy, cross section) shows overview of Ishikawa cells growing as monolayers. B : Lateral cell membranes show tight junctions, adherens junctions and desmosomes in varying combinations, whereas regular junctional complexes consisting of tight junction, adherens junction and desmosomes in apico-basal sequence were rarely seen. C : Ishikawa monolayers after treatment with toxin A. Standard electron microscopy showed no substantial differences between untreated (A) and toxin A-treated cells (C). Me: cell culture medium; N: nucleus; oo: coverslip; *: desmosome; arrow: adherens junction.

Techniques Used: Light Microscopy, Sequencing, Electron Microscopy, Cell Culture

4) Product Images from "Mutation of FIG4 causes a rapidly progressive, asymmetric neuronal degeneration"

Article Title: Mutation of FIG4 causes a rapidly progressive, asymmetric neuronal degeneration

Journal:

doi: 10.1093/brain/awn114

Demyelination confirmed by ultrastructure. Peripheral nerves were prepared into ultrathin sections and examined under EM. ( A ) Sciatic nerves from wild-type mice exhibited myelinated nerve fibres of various diameter as well as non-myelinated fibres. (
Figure Legend Snippet: Demyelination confirmed by ultrastructure. Peripheral nerves were prepared into ultrathin sections and examined under EM. ( A ) Sciatic nerves from wild-type mice exhibited myelinated nerve fibres of various diameter as well as non-myelinated fibres. (

Techniques Used: Mouse Assay

5) Product Images from "The Compensatory G88R Change Is Essential in Restoring the Normal Functions of Influenza A/WSN/33 Virus Matrix Protein 1 with a Disrupted Nuclear Localization Signal"

Article Title: The Compensatory G88R Change Is Essential in Restoring the Normal Functions of Influenza A/WSN/33 Virus Matrix Protein 1 with a Disrupted Nuclear Localization Signal

Journal: Journal of Virology

doi: 10.1128/JVI.02024-12

Transmission electron images of M1 triple mutants. The epoxy resin-embedded M1 triple mutants were cut by an ultramicrotome, and ultrathin sections were stained with uranyl acetate and lead citrate. Images were acquired under a Zeiss EM 912 transmission
Figure Legend Snippet: Transmission electron images of M1 triple mutants. The epoxy resin-embedded M1 triple mutants were cut by an ultramicrotome, and ultrathin sections were stained with uranyl acetate and lead citrate. Images were acquired under a Zeiss EM 912 transmission

Techniques Used: Transmission Assay, Staining

6) Product Images from "In-depth study of Mollivirus sibericum, a new 30,000-y-old giant virus infecting Acanthamoeba"

Article Title: In-depth study of Mollivirus sibericum, a new 30,000-y-old giant virus infecting Acanthamoeba

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1510795112

Ultrathin-section TEM imaging of Mollivirus-infected Acanthamoeba cells. ( A ) Appearance of the nucleus 5 h PI. The nucleolus has almost vanished, filled with fibrillary structures of unknown composition, and the nuclear membrane presents invaginations.
Figure Legend Snippet: Ultrathin-section TEM imaging of Mollivirus-infected Acanthamoeba cells. ( A ) Appearance of the nucleus 5 h PI. The nucleolus has almost vanished, filled with fibrillary structures of unknown composition, and the nuclear membrane presents invaginations.

Techniques Used: Transmission Electron Microscopy, Imaging, Infection

Imaging of Mollivirus particles. ( A ) Scanning electron microscopy of two isolated particles showing the apex structure. ( B ) Transmission electron microscopy (TEM) imaging of an ultrathin section of an open particle after fusion of its internal lipid membrane
Figure Legend Snippet: Imaging of Mollivirus particles. ( A ) Scanning electron microscopy of two isolated particles showing the apex structure. ( B ) Transmission electron microscopy (TEM) imaging of an ultrathin section of an open particle after fusion of its internal lipid membrane

Techniques Used: Imaging, Electron Microscopy, Isolation, Transmission Assay, Transmission Electron Microscopy

7) Product Images from "Cytopathological Effects of Bacillus sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex quinquefasciatus Larvae ▿"

Article Title: Cytopathological Effects of Bacillus sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex quinquefasciatus Larvae ▿

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.00811-09

Transverse ultrathin sections of the posterior midgut from fourth-instar Culex quinquefasciatus larvae resistant to the Bin toxin (CqRL1/2362) treated with Cry48Aa/Cry49Aa toxin from Bacillus sphaericus IAB59. (A) Cell from a nontreated larva with preserved
Figure Legend Snippet: Transverse ultrathin sections of the posterior midgut from fourth-instar Culex quinquefasciatus larvae resistant to the Bin toxin (CqRL1/2362) treated with Cry48Aa/Cry49Aa toxin from Bacillus sphaericus IAB59. (A) Cell from a nontreated larva with preserved

Techniques Used:

Transverse ultrathin sections of the posterior midgut from fourth-instar Culex quinquefasciatus Bin-toxin-susceptible larvae (CqSF) treated with Cry48Aa/Cry49Aa toxin from Bacillus sphaericus IAB59. (A) Cell from a nontreated larva rich in microvilli
Figure Legend Snippet: Transverse ultrathin sections of the posterior midgut from fourth-instar Culex quinquefasciatus Bin-toxin-susceptible larvae (CqSF) treated with Cry48Aa/Cry49Aa toxin from Bacillus sphaericus IAB59. (A) Cell from a nontreated larva rich in microvilli

Techniques Used:

Transverse ultrathin sections of the posterior midgut from fourth-instar Culex quinquefasciatus larvae resistant to Bacillus sphaericus IAB59 (CqRL2/IAB59), treated with Cry48Aa/Cry49Aa toxin from this strain. (A) Basal side of epithelial cell from 6-h-treated
Figure Legend Snippet: Transverse ultrathin sections of the posterior midgut from fourth-instar Culex quinquefasciatus larvae resistant to Bacillus sphaericus IAB59 (CqRL2/IAB59), treated with Cry48Aa/Cry49Aa toxin from this strain. (A) Basal side of epithelial cell from 6-h-treated

Techniques Used:

Transverse ultrathin sections of the posterior midgut from fourth-instar Culex quinquefasciatus larvae resistant to the Bin toxin (CqRL1/2362) treated with a mixture of Bin/Cry11Aa toxins at a 3:1 (wt/wt) ratio. (A) Cell from a nontreated larva showing
Figure Legend Snippet: Transverse ultrathin sections of the posterior midgut from fourth-instar Culex quinquefasciatus larvae resistant to the Bin toxin (CqRL1/2362) treated with a mixture of Bin/Cry11Aa toxins at a 3:1 (wt/wt) ratio. (A) Cell from a nontreated larva showing

Techniques Used:

8) Product Images from "Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus"

Article Title: Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus

Journal: Journal of Virology

doi: 10.1128/JVI.79.3.1635-1644.2005

Visualization of N protein by ultrathin-section immuno-EM. Gold immunolabeling of N protein in SARS-CoV-infected Vero cells with CR3009 (A), CR3018 (B), or negative control MAb (C) was carried out, followed by incubation with 5-nm-colloidal-gold-conjugated secondary antibody.
Figure Legend Snippet: Visualization of N protein by ultrathin-section immuno-EM. Gold immunolabeling of N protein in SARS-CoV-infected Vero cells with CR3009 (A), CR3018 (B), or negative control MAb (C) was carried out, followed by incubation with 5-nm-colloidal-gold-conjugated secondary antibody.

Techniques Used: Immunolabeling, Infection, Negative Control, Incubation

9) Product Images from "Ultrastructural and Biochemical Alterations Induced by 22,26-Azasterol, a ?24(25)-Sterol Methyltransferase Inhibitor, on Promastigote and Amastigote Forms of Leishmania amazonensis"

Article Title: Ultrastructural and Biochemical Alterations Induced by 22,26-Azasterol, a ?24(25)-Sterol Methyltransferase Inhibitor, on Promastigote and Amastigote Forms of Leishmania amazonensis

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.46.2.487-499.2002

FIG. 21-22. Promastigote treated with 10 μM azasterol for 24 h presented a myelin-like figure in the cytoplasm (arrows). Bar, 0.5 μm. Ultrathin section of promastigote treated with 0.1 μM azasterol for 48 h showing proliferation in the number of autophagosomal structures (asterisks). Bar, 0.5 μm.
Figure Legend Snippet: FIG. 21-22. Promastigote treated with 10 μM azasterol for 24 h presented a myelin-like figure in the cytoplasm (arrows). Bar, 0.5 μm. Ultrathin section of promastigote treated with 0.1 μM azasterol for 48 h showing proliferation in the number of autophagosomal structures (asterisks). Bar, 0.5 μm.

Techniques Used:

10) Product Images from "Dynamic Acquisition and Loss of Dual-Obligate Symbionts in the Plant-Sap-Feeding Adelgidae (Hemiptera: Sternorrhyncha: Aphidoidea)"

Article Title: Dynamic Acquisition and Loss of Dual-Obligate Symbionts in the Plant-Sap-Feeding Adelgidae (Hemiptera: Sternorrhyncha: Aphidoidea)

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2017.01037

Ultrastructure of Pineus pini endosymbionts residing in bacteriocytes. (A) Ultrathin section of bacteriome in whole-mount insects, showing two distinct bacteriocytes containing ‘ Ca . Annandia pinicola’ (left) and ‘ Ca . Hartigia pinicola’ (right). (B) High magnification of ‘ Ca . Annandia pinicola’ cell envelope, comprising three membrane layers, presumably corresponding to inner and outer membranes and a symbiosome membrane. (C) High magnification of ‘ Ca . Hartigia pinicola’ cell envelope, comprising three membrane layers, presumably corresponding to inner and outer membranes and a symbiosome membrane; no peptidoglycan layer is apparent. Ap, ‘ Ca . Annandia pinicola’; Hp, ‘ Ca . Hartigia pinicola’; n, bacteriocyte nucleus.
Figure Legend Snippet: Ultrastructure of Pineus pini endosymbionts residing in bacteriocytes. (A) Ultrathin section of bacteriome in whole-mount insects, showing two distinct bacteriocytes containing ‘ Ca . Annandia pinicola’ (left) and ‘ Ca . Hartigia pinicola’ (right). (B) High magnification of ‘ Ca . Annandia pinicola’ cell envelope, comprising three membrane layers, presumably corresponding to inner and outer membranes and a symbiosome membrane. (C) High magnification of ‘ Ca . Hartigia pinicola’ cell envelope, comprising three membrane layers, presumably corresponding to inner and outer membranes and a symbiosome membrane; no peptidoglycan layer is apparent. Ap, ‘ Ca . Annandia pinicola’; Hp, ‘ Ca . Hartigia pinicola’; n, bacteriocyte nucleus.

Techniques Used:

11) Product Images from "Large-scale tissue clearing (PACT): Technical evaluation and new perspectives in immunofluorescence, histology, and ultrastructure"

Article Title: Large-scale tissue clearing (PACT): Technical evaluation and new perspectives in immunofluorescence, histology, and ultrastructure

Journal: Scientific Reports

doi: 10.1038/srep34331

Transmission electron microscopy of cleared tissue. Shown are ultrathin sections of specimens fixed and contrasted with osmium only ( A–D left ), and of tissues cleared previously with CLARITY solution ( A,B middle ) and PACT solution ( A–D right ). In A , overviews of enterocytes are depicted, below are high resolution images of enterocyte nuclei ( B ), apical microvilli ( C ) and subapical junctional complex ( D ). Lipids were washed out of all cleared specimen indicated by the lack of stained membranes. Tissues cleared in CLARITY solution exhibited shrinking artefacts and an enlarged perinuclear cleft ( B, arrows ) as well as a loss of fine structures especially at the apical part of the enterocytes. Tissues treated with PACT solution retained fine structural features like actin filaments in microvilli ( C left ) desmosomes ( D, arrows ) although the membranes are lost. Scale bars: A and B 1 μm, C and D 500 nm, for all images in the same row, respectively.
Figure Legend Snippet: Transmission electron microscopy of cleared tissue. Shown are ultrathin sections of specimens fixed and contrasted with osmium only ( A–D left ), and of tissues cleared previously with CLARITY solution ( A,B middle ) and PACT solution ( A–D right ). In A , overviews of enterocytes are depicted, below are high resolution images of enterocyte nuclei ( B ), apical microvilli ( C ) and subapical junctional complex ( D ). Lipids were washed out of all cleared specimen indicated by the lack of stained membranes. Tissues cleared in CLARITY solution exhibited shrinking artefacts and an enlarged perinuclear cleft ( B, arrows ) as well as a loss of fine structures especially at the apical part of the enterocytes. Tissues treated with PACT solution retained fine structural features like actin filaments in microvilli ( C left ) desmosomes ( D, arrows ) although the membranes are lost. Scale bars: A and B 1 μm, C and D 500 nm, for all images in the same row, respectively.

Techniques Used: Transmission Assay, Electron Microscopy, Staining

12) Product Images from "Trypanosoma cruzi Epimastigotes Are Able to Store and Mobilize High Amounts of Cholesterol in Reservosome Lipid Inclusions"

Article Title: Trypanosoma cruzi Epimastigotes Are Able to Store and Mobilize High Amounts of Cholesterol in Reservosome Lipid Inclusions

Journal: PLoS ONE

doi: 10.1371/journal.pone.0022359

The neutral lipid stock inside epimastigotes decreases along time in serum-free medium. The Figure shows the absence of fluorescence almost complete in epimastigotes from 10 (B) and 50% FCS (E) after 48 h of serum starvation. Ultrathin sections revealed that the majority of reservosomes are devoid of lipid inclusions, while others still present rectangular lipid profiles in 10 (C) and 50% (F). Merged images of DIC and DAPI fluorescence were used to point nucleus and kinetoplast position (A, C). The asterisks indicate the lipid inclusions. Bars: 10 µm (a–B, D–E) and 0.5 µm (C–F).
Figure Legend Snippet: The neutral lipid stock inside epimastigotes decreases along time in serum-free medium. The Figure shows the absence of fluorescence almost complete in epimastigotes from 10 (B) and 50% FCS (E) after 48 h of serum starvation. Ultrathin sections revealed that the majority of reservosomes are devoid of lipid inclusions, while others still present rectangular lipid profiles in 10 (C) and 50% (F). Merged images of DIC and DAPI fluorescence were used to point nucleus and kinetoplast position (A, C). The asterisks indicate the lipid inclusions. Bars: 10 µm (a–B, D–E) and 0.5 µm (C–F).

Techniques Used: Fluorescence

Isolation of reservosome lipid inclusions. ( A ) DIC and ( B ) Nile Red fluorescence of reservosomes isolated from epimastigotes cultivated with 10% of FCS. ( C ) Ultrathin section of an isolated reservosome. ( D , E , F ) Whole mount osmium tetroxide and uranyl acetate-stained electron microscopy of the reservosome lipid inclusion fraction, showing rectangular and spherical forms. Note the morphological similarity between isolated inclusions and those inside intact reservosomes (C). Bars (A, B) 5 µm, (C) 0.15 µm, (D, E) 0.2 µm, (F) 0.15 µm.
Figure Legend Snippet: Isolation of reservosome lipid inclusions. ( A ) DIC and ( B ) Nile Red fluorescence of reservosomes isolated from epimastigotes cultivated with 10% of FCS. ( C ) Ultrathin section of an isolated reservosome. ( D , E , F ) Whole mount osmium tetroxide and uranyl acetate-stained electron microscopy of the reservosome lipid inclusion fraction, showing rectangular and spherical forms. Note the morphological similarity between isolated inclusions and those inside intact reservosomes (C). Bars (A, B) 5 µm, (C) 0.15 µm, (D, E) 0.2 µm, (F) 0.15 µm.

Techniques Used: Isolation, Fluorescence, Staining, Electron Microscopy

13) Product Images from "The metatrochophore of a deep-sea hydrothermal vent vestimentiferan (Polychaeta: Siboglinidae)"

Article Title: The metatrochophore of a deep-sea hydrothermal vent vestimentiferan (Polychaeta: Siboglinidae)

Journal: Organisms, Diversity & Evolution

doi: 10.1007/s13127-012-0117-z

Serial ultrathin sections of uncinus not extending above cuticle of specimen #308. a , b Uncinus with capitium (ca), subrostral process (sp) surrounded by epidermis cell with nucleus (ne1); c , d uncinus with capitium (ca), subrostral process (sp), epidermis cell with nucleus (ne1) surrounded by follicle cell with nuclei (nf1); e , f chaetoblast with nucleus (nc) and manubrium (ma), surrounded by follicle cells (nf1, nf2); g , h chaetoblast with nucleus (nc), granules (gr), and microvilli (mv) shaping the manubrium
Figure Legend Snippet: Serial ultrathin sections of uncinus not extending above cuticle of specimen #308. a , b Uncinus with capitium (ca), subrostral process (sp) surrounded by epidermis cell with nucleus (ne1); c , d uncinus with capitium (ca), subrostral process (sp), epidermis cell with nucleus (ne1) surrounded by follicle cell with nuclei (nf1); e , f chaetoblast with nucleus (nc) and manubrium (ma), surrounded by follicle cells (nf1, nf2); g , h chaetoblast with nucleus (nc), granules (gr), and microvilli (mv) shaping the manubrium

Techniques Used:

Series of ultrathin sections through blind-ending coelomic duct adjacent to foregut (fg) from posterior to anterior in first segment in specimen #308. a Basal portion of coelomic duct with cilia (ci) built by two ciliated mesodermal cells m1 and m2 with nuclei (nm1, nm2); b larger duct with cilia (ci) surrounded by mesodermal cells m3 and one of the two ciliated mesodermal cells m2 with nucleus (nm2); c larger duct with cilia (ci) surrounded by mesodermal cells m3 and m4, nucleus of m3 (nm3); d anterior blind-ending of small duct with a few cilia (ci) surrounded by several mesodermal cells (m 4-7) partly with nuclei (nm 5-7)
Figure Legend Snippet: Series of ultrathin sections through blind-ending coelomic duct adjacent to foregut (fg) from posterior to anterior in first segment in specimen #308. a Basal portion of coelomic duct with cilia (ci) built by two ciliated mesodermal cells m1 and m2 with nuclei (nm1, nm2); b larger duct with cilia (ci) surrounded by mesodermal cells m3 and one of the two ciliated mesodermal cells m2 with nucleus (nm2); c larger duct with cilia (ci) surrounded by mesodermal cells m3 and m4, nucleus of m3 (nm3); d anterior blind-ending of small duct with a few cilia (ci) surrounded by several mesodermal cells (m 4-7) partly with nuclei (nm 5-7)

Techniques Used:

Cross sections of ultrathin series from anterior to posterior, left ventral, right dorsal; specimen #308. a , b Prostomium with brain (nec) and unpaired, small coelomic cavity (asterisk) peristomium (pe) with prototroch (pr); two dorsally located tentacles (te); c prostomium with unpaired, small coelomic cavity (asterisk) between trochoblasts of prototroch, mouth opening (mo), brain (b) with neuropil; two tentacles (te); d first segment with foregut (fg), mesoderm (me) and mesoderm of tentacles (mte); e first segment with foregut (fg) ventral (vv) and dorsal blood vessel (dv), pyriform gland (py), mesoderm (me), somatic muscle cells (mu); f first segment with transition from foregut (fg) to midgut (mg) and ventral blood vessel (vv); g first and second segment with uncini (un), foregut (fg) and midgut (mg); h first to third segment (s1-s3) with midgut (mg) and hindgut (hg), pyriform gland (py); i first to third segment with anus (a), midgut (mg), and dorsal blood vessel (dv)
Figure Legend Snippet: Cross sections of ultrathin series from anterior to posterior, left ventral, right dorsal; specimen #308. a , b Prostomium with brain (nec) and unpaired, small coelomic cavity (asterisk) peristomium (pe) with prototroch (pr); two dorsally located tentacles (te); c prostomium with unpaired, small coelomic cavity (asterisk) between trochoblasts of prototroch, mouth opening (mo), brain (b) with neuropil; two tentacles (te); d first segment with foregut (fg), mesoderm (me) and mesoderm of tentacles (mte); e first segment with foregut (fg) ventral (vv) and dorsal blood vessel (dv), pyriform gland (py), mesoderm (me), somatic muscle cells (mu); f first segment with transition from foregut (fg) to midgut (mg) and ventral blood vessel (vv); g first and second segment with uncini (un), foregut (fg) and midgut (mg); h first to third segment (s1-s3) with midgut (mg) and hindgut (hg), pyriform gland (py); i first to third segment with anus (a), midgut (mg), and dorsal blood vessel (dv)

Techniques Used:

Tentacles. a Longitudinal ultrathin section of distal tentacle in specimen #675 with coelomic cavity (ct) surrounded by myoepithelial cells (mte), parts of prototroch (pr) ventral to tentacle; b – e series of ultrathin sections through region of connection between mesoderm of tentacles and first segment of specimen #308; arrows point to ECM of mesoderm; b epithelio-muscle cells (mu) surrounded by epidermis in tentacle; c solid mesoderm strand (mte) surrounded by epidermis in tentacle; d , e merging of mesoderm of first segment (m1) with mesoderm of tentacle (mte), foregut (fg)
Figure Legend Snippet: Tentacles. a Longitudinal ultrathin section of distal tentacle in specimen #675 with coelomic cavity (ct) surrounded by myoepithelial cells (mte), parts of prototroch (pr) ventral to tentacle; b – e series of ultrathin sections through region of connection between mesoderm of tentacles and first segment of specimen #308; arrows point to ECM of mesoderm; b epithelio-muscle cells (mu) surrounded by epidermis in tentacle; c solid mesoderm strand (mte) surrounded by epidermis in tentacle; d , e merging of mesoderm of first segment (m1) with mesoderm of tentacle (mte), foregut (fg)

Techniques Used:

Longitudinal sections, ultrathin of specimen #542 ( a , b ) and semithin of specimen #675 ( c , d ). a Prostomium anterior to peristomium with prototroch (pr), tentacle (te), foregut (fg), and midgut (mg) with adjacent ventral blood vessel (vv); b prostomium with unpaired coelomic cavity (cp) anterior to peristomium with prototroch (pr) and mouth opening (mo), segment 1 with tentacle (te), foregut (fg), and midgut (mg); c prostomium and peristomium with mouth opening (mo), segment 1 with tentacle (te), foregut (fg) with adjacent dorsal (dv) and ventral blood vessel (vv), midgut (mg), trophosome (tr), and coelomic cavity (c1); segment 2 with coelomic cavity (c2); d prostomium with coelomic cavity (cp), segment 1 with tentacle (te), trophosome (tr), midgut (mg), and coelomic cavity (c1)
Figure Legend Snippet: Longitudinal sections, ultrathin of specimen #542 ( a , b ) and semithin of specimen #675 ( c , d ). a Prostomium anterior to peristomium with prototroch (pr), tentacle (te), foregut (fg), and midgut (mg) with adjacent ventral blood vessel (vv); b prostomium with unpaired coelomic cavity (cp) anterior to peristomium with prototroch (pr) and mouth opening (mo), segment 1 with tentacle (te), foregut (fg), and midgut (mg); c prostomium and peristomium with mouth opening (mo), segment 1 with tentacle (te), foregut (fg) with adjacent dorsal (dv) and ventral blood vessel (vv), midgut (mg), trophosome (tr), and coelomic cavity (c1); segment 2 with coelomic cavity (c2); d prostomium with coelomic cavity (cp), segment 1 with tentacle (te), trophosome (tr), midgut (mg), and coelomic cavity (c1)

Techniques Used:

14) Product Images from "AthPEX10, a nuclear gene essential for peroxisome and storage organelle formation during Arabidopsis embryogenesis"

Article Title: AthPEX10, a nuclear gene essential for peroxisome and storage organelle formation during Arabidopsis embryogenesis

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1633697100

Electron micrographs of a ultrathin section through the meristematic cells of a lethal embryo. The rough ER forms an elaborate network of “swollen” strands and cisternae ( A ). The electron translucent lumen of the ER becomes filled with amorphous, fibrous material ( B ), which finally fills storage vacuoles of similar size as wild-type protein bodies ( C , asterisks). Abnormal lipid body formation is a characteristic feature: extemly thin “lipid body disks” form stacks of several disks ( D ). The flat “lipid bodies” have a tripartite structure: two electron-dense half-unit membranes and a homogeneous, electron-translucent matrix presumably formed by triacylglycerols. The thickness of the lipid matrix of the flat disks is in the range of 10 nm but can differ widely ( E ). Little lipid bodies (LB) can apparently be assembled by the ER in the absence of PEX10p and are reminiscent of nascent lipid bodies (oleosomes) found at the torpedo stage ( B and F ). CW, cell wall.
Figure Legend Snippet: Electron micrographs of a ultrathin section through the meristematic cells of a lethal embryo. The rough ER forms an elaborate network of “swollen” strands and cisternae ( A ). The electron translucent lumen of the ER becomes filled with amorphous, fibrous material ( B ), which finally fills storage vacuoles of similar size as wild-type protein bodies ( C , asterisks). Abnormal lipid body formation is a characteristic feature: extemly thin “lipid body disks” form stacks of several disks ( D ). The flat “lipid bodies” have a tripartite structure: two electron-dense half-unit membranes and a homogeneous, electron-translucent matrix presumably formed by triacylglycerols. The thickness of the lipid matrix of the flat disks is in the range of 10 nm but can differ widely ( E ). Little lipid bodies (LB) can apparently be assembled by the ER in the absence of PEX10p and are reminiscent of nascent lipid bodies (oleosomes) found at the torpedo stage ( B and F ). CW, cell wall.

Techniques Used:

Electron micrographs of a ultrathin section through a wild-type torpedo-stage embryo. Electron translucent lipid bodies (LB) are formed in the cytoplasm, which is tightly packed with ribosomes ( A ). Rough ER is in connection with spherical lipid bodies ( B and C ). The developing lipid bodies are characterized by a surface of regular spaced channels ( D , arrows) with the same electron density as the triacylglycerols of the lipid bodies. N, nucleus; n, nucleolus; P, chloroplast; S, starch grain; V, vacuole.
Figure Legend Snippet: Electron micrographs of a ultrathin section through a wild-type torpedo-stage embryo. Electron translucent lipid bodies (LB) are formed in the cytoplasm, which is tightly packed with ribosomes ( A ). Rough ER is in connection with spherical lipid bodies ( B and C ). The developing lipid bodies are characterized by a surface of regular spaced channels ( D , arrows) with the same electron density as the triacylglycerols of the lipid bodies. N, nucleus; n, nucleolus; P, chloroplast; S, starch grain; V, vacuole.

Techniques Used:

15) Product Images from "Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants"

Article Title: Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as Mucosal Adjuvants

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2020.00015

Transmission electron microscopy analysis. (A) Lactobacillus rhamnosus CRL1505 untreated cells and (B) bacterium-like particles obtained from L. rhamnosus CRL1505 (IBLP1505) were fixed with Karnovsky fixative, postfixed with 1% osmium tetroxide in sodium phosphate buffer and embedded in Spurr resin. Ultrathin sections cuts were examined with a Zeiss libra 120 Transmission Electron Microscope.
Figure Legend Snippet: Transmission electron microscopy analysis. (A) Lactobacillus rhamnosus CRL1505 untreated cells and (B) bacterium-like particles obtained from L. rhamnosus CRL1505 (IBLP1505) were fixed with Karnovsky fixative, postfixed with 1% osmium tetroxide in sodium phosphate buffer and embedded in Spurr resin. Ultrathin sections cuts were examined with a Zeiss libra 120 Transmission Electron Microscope.

Techniques Used: Transmission Assay, Electron Microscopy, Microscopy

16) Product Images from "Specific Features of Mandible Structure and Elemental Composition in the Polyphagous Amphipod Acanthogammarus grewingkii Endemic to Lake Baikal"

Article Title: Specific Features of Mandible Structure and Elemental Composition in the Polyphagous Amphipod Acanthogammarus grewingkii Endemic to Lake Baikal

Journal: PLoS ONE

doi: 10.1371/journal.pone.0043073

Mandible structure and elemental spectrum of the tooth area. ( A ) Acanthogammarus grewingkii, external view. The star indicates the centrolateral part of P1 where the AFM and TEM images were taken and XEPMA measurements of elemental composition were made; the circle indicates the location of MD. ( B ) The distal part of the left MD (SEM). Arrowheads indicate pores. ( C ) The first IN teeth on the left MD (SEM). The arrow indicates the boundary between the cutting edge and corpus of MD. ( D ) The cuticular surface of P1 (AFM). Arrowheads indicate pores. ( E ) The surface of an IN tooth with signs of wear (AFM). ( F ) An ultrathin section through the EP of P1 (TEM). Arrows indicate pores. ( G ) An ultrathin section through the external EP of an IN tooth (TEM). ( H ) X-Ray elemental spectrum of the mapped IN tooth area (SEM-EDS). ( I ) Apical parts of IN teeth on the right MD, top view. Circles indicate Br-depleted areas (SEM-EDS). ( J ) The apical part of IN tooth with traces of wearing, top view (SEM). ( K ) X-Ray map of Br distribution in the apical part of an IN tooth. Arrows indicate Br-depleted areas (SEM-EDS). ( L ) The layered structure of EP in a section through the IN tooth apex (image in back-scattered electrons in a Superprobe JXA-8200 scanning electron microscope). Stars indicate points of elemental composition measurements.
Figure Legend Snippet: Mandible structure and elemental spectrum of the tooth area. ( A ) Acanthogammarus grewingkii, external view. The star indicates the centrolateral part of P1 where the AFM and TEM images were taken and XEPMA measurements of elemental composition were made; the circle indicates the location of MD. ( B ) The distal part of the left MD (SEM). Arrowheads indicate pores. ( C ) The first IN teeth on the left MD (SEM). The arrow indicates the boundary between the cutting edge and corpus of MD. ( D ) The cuticular surface of P1 (AFM). Arrowheads indicate pores. ( E ) The surface of an IN tooth with signs of wear (AFM). ( F ) An ultrathin section through the EP of P1 (TEM). Arrows indicate pores. ( G ) An ultrathin section through the external EP of an IN tooth (TEM). ( H ) X-Ray elemental spectrum of the mapped IN tooth area (SEM-EDS). ( I ) Apical parts of IN teeth on the right MD, top view. Circles indicate Br-depleted areas (SEM-EDS). ( J ) The apical part of IN tooth with traces of wearing, top view (SEM). ( K ) X-Ray map of Br distribution in the apical part of an IN tooth. Arrows indicate Br-depleted areas (SEM-EDS). ( L ) The layered structure of EP in a section through the IN tooth apex (image in back-scattered electrons in a Superprobe JXA-8200 scanning electron microscope). Stars indicate points of elemental composition measurements.

Techniques Used: Transmission Electron Microscopy, Microscopy

17) Product Images from "Structural Changes of the Paraflagellar Rod during Flagellar Beating in Trypanosoma cruzi"

Article Title: Structural Changes of the Paraflagellar Rod during Flagellar Beating in Trypanosoma cruzi

Journal: PLoS ONE

doi: 10.1371/journal.pone.0011407

Electron micrographs of epimastigote form of T. cruzi . (a) Scanning electron micrograph of T. cruzi . The flagellum (arrowheads), which emerges from the flagellar pocket (arrow), is attached to the cell body along the flagellar attachment zone (FAZ) region. (b–c) Transmission electron micrographs of ultrathin sections of epimastigote forms. The axoneme (A) and paraflagellar rod (*) are seen in both longitudinal (b) and transversal (c) sections. In longitudinal sections, it is clear that the flagellum emerges from the flagellar pocket (thick arrow). FAZ region (arrows) and subpellicular microtubules (arrowheads in c) can also be observed. (N) Nucleus, (K) kinetoplast, (G) Golgi complex, (M) mitochondria. Bars: a – 5 µm; b – 1 µm; c – 100 nm.
Figure Legend Snippet: Electron micrographs of epimastigote form of T. cruzi . (a) Scanning electron micrograph of T. cruzi . The flagellum (arrowheads), which emerges from the flagellar pocket (arrow), is attached to the cell body along the flagellar attachment zone (FAZ) region. (b–c) Transmission electron micrographs of ultrathin sections of epimastigote forms. The axoneme (A) and paraflagellar rod (*) are seen in both longitudinal (b) and transversal (c) sections. In longitudinal sections, it is clear that the flagellum emerges from the flagellar pocket (thick arrow). FAZ region (arrows) and subpellicular microtubules (arrowheads in c) can also be observed. (N) Nucleus, (K) kinetoplast, (G) Golgi complex, (M) mitochondria. Bars: a – 5 µm; b – 1 µm; c – 100 nm.

Techniques Used: Transmission Assay

18) Product Images from "Panglial Gap Junctional Communication is Essential for Maintenance of Myelin in the CNS"

Article Title: Panglial Gap Junctional Communication is Essential for Maintenance of Myelin in the CNS

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.0392-12.2012

White matter pathology in cerebellum and corpus callosum of P80–P90 Cx30 −/− /Cx47 −/− mice. A , B , Toluidine blue-stained 2 μm epoxy sections of tissues revealed severe vacuolization of cerebellar white matter in Cx30 −/− /Cx47 −/− mice compared to Cx30 +/− /Cx47 +/− controls. C , D , Ultrathin sections of cerebellar white matter revealed vacuoles located at the outer layer of myelin in Cx30 −/− /Cx47 −/− mice (asterisks) that were not observed in Cx30 +/− /Cx47 +/− animals. E , An oligodendrocyte identified by nuclei with clumped heterochromatin and electron-dense cytoplasm in the corpus callosum of Cx30 +/− /Cx47 +/− animals. F , Electron micrograph shows vacuoles (asterisks) accompanied by unfolded myelin in the corpus callosum of Cx30 −/− /Cx47 −/− mice. G , H , Graphs indicate g ratio plotted versus axon diameter. In both corpus callosum ( G ) and cerebellar white matter ( H ), Cx30 −/− /Cx47 −/− mice display a significantly higher g ratio compared to Cx30 +/− /Cx47 +/− mice (Mann–Whitney U test, p
Figure Legend Snippet: White matter pathology in cerebellum and corpus callosum of P80–P90 Cx30 −/− /Cx47 −/− mice. A , B , Toluidine blue-stained 2 μm epoxy sections of tissues revealed severe vacuolization of cerebellar white matter in Cx30 −/− /Cx47 −/− mice compared to Cx30 +/− /Cx47 +/− controls. C , D , Ultrathin sections of cerebellar white matter revealed vacuoles located at the outer layer of myelin in Cx30 −/− /Cx47 −/− mice (asterisks) that were not observed in Cx30 +/− /Cx47 +/− animals. E , An oligodendrocyte identified by nuclei with clumped heterochromatin and electron-dense cytoplasm in the corpus callosum of Cx30 +/− /Cx47 +/− animals. F , Electron micrograph shows vacuoles (asterisks) accompanied by unfolded myelin in the corpus callosum of Cx30 −/− /Cx47 −/− mice. G , H , Graphs indicate g ratio plotted versus axon diameter. In both corpus callosum ( G ) and cerebellar white matter ( H ), Cx30 −/− /Cx47 −/− mice display a significantly higher g ratio compared to Cx30 +/− /Cx47 +/− mice (Mann–Whitney U test, p

Techniques Used: Mouse Assay, Staining, MANN-WHITNEY

19) Product Images from "Identification and location of bone-forming cells within cartilage canals on their course into the secondary ossification centre"

Article Title: Identification and location of bone-forming cells within cartilage canals on their course into the secondary ossification centre

Journal: Journal of Anatomy

doi: 10.1111/j.1469-7580.2006.00578.x

Transmission electron micrographs. (A,B) Ultrathin sections through a cartilage canal (cc) outside the SOC within the reserve zone of the epiphysis (D28). The electron-translucent matrix (cm) of the canal contains numerous cross-banded collagen fibrils.
Figure Legend Snippet: Transmission electron micrographs. (A,B) Ultrathin sections through a cartilage canal (cc) outside the SOC within the reserve zone of the epiphysis (D28). The electron-translucent matrix (cm) of the canal contains numerous cross-banded collagen fibrils.

Techniques Used: Transmission Assay

20) Product Images from "The Pine Bark Adelgid, Pineus strobi, Contains Two Novel Bacteriocyte-Associated Gammaproteobacterial Symbionts"

Article Title: The Pine Bark Adelgid, Pineus strobi, Contains Two Novel Bacteriocyte-Associated Gammaproteobacterial Symbionts

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.03310-13

Ultrastructure of bacteriocyte-associated symbionts of Pineus strobi . (A and B) Ultrathin sections of the adelgid abdomen showing two symbiont morphotypes located in distinct bacteriocytes. (C to E) The coccoid (phylotype 1) (C and D) and the polymorphic
Figure Legend Snippet: Ultrastructure of bacteriocyte-associated symbionts of Pineus strobi . (A and B) Ultrathin sections of the adelgid abdomen showing two symbiont morphotypes located in distinct bacteriocytes. (C to E) The coccoid (phylotype 1) (C and D) and the polymorphic

Techniques Used:

21) Product Images from "The Aspergillus fumigatus Transcription Factor Ace2 Governs Pigment Production, Conidiation and Virulence"

Article Title: The Aspergillus fumigatus Transcription Factor Ace2 Governs Pigment Production, Conidiation and Virulence

Journal: Molecular microbiology

doi: 10.1111/j.1365-2958.2009.06631.x

ace2 is necessary for the normal cell wall architecture of A. fumigatus . (A-F) Transmission electron micrographs of ultrathin sections of conidia of the indicated strains of A. fumigatus . (A-C) Lower power images showing an entire conidium. Small black
Figure Legend Snippet: ace2 is necessary for the normal cell wall architecture of A. fumigatus . (A-F) Transmission electron micrographs of ultrathin sections of conidia of the indicated strains of A. fumigatus . (A-C) Lower power images showing an entire conidium. Small black

Techniques Used: Transmission Assay

22) Product Images from "Cellular and Subcellular Aquaporin-4 Distribution in the Mouse Neurohypophysis and the Effects of Osmotic Stimulation"

Article Title: Cellular and Subcellular Aquaporin-4 Distribution in the Mouse Neurohypophysis and the Effects of Osmotic Stimulation

Journal: Journal of Histochemistry and Cytochemistry

doi: 10.1369/jhc.2010.956805

Ultrathin section of the neurohypophysis showing aquaporin-4 (AQP4) immunolabeling in a blood vessel and its surroundings in control (A) and 8-day salt-loaded mice (B). In the control (A), the perivascular space is small with few nerve terminals containing labeled microvesicles, forming electron-dense clusters (arrow). In the salt-loaded mouse (B), the perivascular space is swollen and occupied by large nerve terminals rich in labeled neurosecretory granules and microvesicles (arrow). BV, blood vessel; E, endothelial cell; NT, nerve terminal; PVS, perivascular space. Scale bar: 2 µm (A); 1 µm (B).
Figure Legend Snippet: Ultrathin section of the neurohypophysis showing aquaporin-4 (AQP4) immunolabeling in a blood vessel and its surroundings in control (A) and 8-day salt-loaded mice (B). In the control (A), the perivascular space is small with few nerve terminals containing labeled microvesicles, forming electron-dense clusters (arrow). In the salt-loaded mouse (B), the perivascular space is swollen and occupied by large nerve terminals rich in labeled neurosecretory granules and microvesicles (arrow). BV, blood vessel; E, endothelial cell; NT, nerve terminal; PVS, perivascular space. Scale bar: 2 µm (A); 1 µm (B).

Techniques Used: Immunolabeling, Mouse Assay, Labeling

Ultrathin section of the neurohypophysis showing aquaporin-4 (AQP4) immunostaining in a pituicyte and its surroundings in control (A) and 8-day salt-loaded mice (B). In the control (A), pituicytes present a large nucleus and clear cytoplasm. Their plasma membrane is in tight contact with numerous labeled nerve fibers from hypothalamic magnocellular neurons, which contain many labeled neurosecretory granules (arrow). In the salt-loaded mouse (B), the nucleus and cytoplasm of pituicytes are denser, and nerve terminals around pituicytes exhibit fewer dense labeled neurosecretory vesicles (arrow) but more abundant clear vesicles. BV, blood vessel; N, nucleus; P, pituicytes. Scale bar: 2 µm.
Figure Legend Snippet: Ultrathin section of the neurohypophysis showing aquaporin-4 (AQP4) immunostaining in a pituicyte and its surroundings in control (A) and 8-day salt-loaded mice (B). In the control (A), pituicytes present a large nucleus and clear cytoplasm. Their plasma membrane is in tight contact with numerous labeled nerve fibers from hypothalamic magnocellular neurons, which contain many labeled neurosecretory granules (arrow). In the salt-loaded mouse (B), the nucleus and cytoplasm of pituicytes are denser, and nerve terminals around pituicytes exhibit fewer dense labeled neurosecretory vesicles (arrow) but more abundant clear vesicles. BV, blood vessel; N, nucleus; P, pituicytes. Scale bar: 2 µm.

Techniques Used: Immunostaining, Mouse Assay, Labeling

23) Product Images from "Isolation of Novel Ultramicrobacteria Classified as Actinobacteria from Five Freshwater Habitats in Europe and Asia"

Article Title: Isolation of Novel Ultramicrobacteria Classified as Actinobacteria from Five Freshwater Habitats in Europe and Asia

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.69.3.1442-1451.2003

(a and b) Survey views of ultrathin-sectioned (a) and shadow-cast (b) cells of strain MWH-Ta1. The small arrowheads indicate sites of binary fission. The large arrowhead in panel b indicates the direction of shadow casting. (c) Detailed view of a cross section of the cell wall. The cytoplasm (cp), the cytoplasmic membrane (cm), and the outer surface-like layer (sl) are indicated. The area enclosed by a box represents the measured area, and an averaged line scan density profile of this area is shown in panel d. The density features of the cell wall are shown in the area enclosed by a box in panel d. pg, peptidoglycan.
Figure Legend Snippet: (a and b) Survey views of ultrathin-sectioned (a) and shadow-cast (b) cells of strain MWH-Ta1. The small arrowheads indicate sites of binary fission. The large arrowhead in panel b indicates the direction of shadow casting. (c) Detailed view of a cross section of the cell wall. The cytoplasm (cp), the cytoplasmic membrane (cm), and the outer surface-like layer (sl) are indicated. The area enclosed by a box represents the measured area, and an averaged line scan density profile of this area is shown in panel d. The density features of the cell wall are shown in the area enclosed by a box in panel d. pg, peptidoglycan.

Techniques Used:

24) Product Images from "Chronic Intake of Green Propolis Negatively Affecting the Rat Testis"

Article Title: Chronic Intake of Green Propolis Negatively Affecting the Rat Testis

Journal: Pharmacognosy Research

doi: 10.4103/0974-8490.199777

Ultrathin testicular sections of the control animals analyzed by transmission electron microscopy. Sertoli cell supported by a basal lamina. Numerous lipid droplets, endoplasmic reticulum swelling, and electron dense materials are observed in the cytoplasm of the Sertoli cells (a). Spermatocytes and round spermatids at seminiferous tubules are closely linked (b). Leydig cells in the testes of the control group showed lipid droplets, mitochondria, and Golgi apparatus (3c). Barr = 10 μm (a), 5 μm (b and c). SC: Sertoli cell; bl: Basal lamina; ld: Lipid droplets; ER: Endoplasmic reticulum swelling; dm: Dense materials; sp: Spermatocytes; spm: Spermatids; mi: Mitochondria; GA: Golgi apparatus
Figure Legend Snippet: Ultrathin testicular sections of the control animals analyzed by transmission electron microscopy. Sertoli cell supported by a basal lamina. Numerous lipid droplets, endoplasmic reticulum swelling, and electron dense materials are observed in the cytoplasm of the Sertoli cells (a). Spermatocytes and round spermatids at seminiferous tubules are closely linked (b). Leydig cells in the testes of the control group showed lipid droplets, mitochondria, and Golgi apparatus (3c). Barr = 10 μm (a), 5 μm (b and c). SC: Sertoli cell; bl: Basal lamina; ld: Lipid droplets; ER: Endoplasmic reticulum swelling; dm: Dense materials; sp: Spermatocytes; spm: Spermatids; mi: Mitochondria; GA: Golgi apparatus

Techniques Used: Transmission Assay, Electron Microscopy

Ultrathin testicular sections of the exposed groups analyzed by transmission electron microscopy. Sertoli cell supported by a basal lamina. Numerous lipid droplets, endoplasmic reticulum swelling, and electron-dense materials are observed in the cytoplasm of the Sertoli cells (a and b). Damage to the structure of the blood–testis barrier can be observed in T3 (b), empty spaces between Sertoli cells and spermatids were clearly visible after propolis exposure (c and d). Residual bodies in the cytoplasm of Sertoli cells (d). Spermatozoa with accumulated cytoplasm (d) and head and tail abnormalities (d). Barr = 10 μm (a and d), 5 μm (b, c, and e), 2 μm (f). SC: Sertoli cell; bl: Basal lamina; ld: Lipid droplets; ER: Endoplasmic reticulum swelling; dm: Dense materials; BTB: Blood–testis barrier; rb: Residual bodies; ac: Accumulated cytoplasm
Figure Legend Snippet: Ultrathin testicular sections of the exposed groups analyzed by transmission electron microscopy. Sertoli cell supported by a basal lamina. Numerous lipid droplets, endoplasmic reticulum swelling, and electron-dense materials are observed in the cytoplasm of the Sertoli cells (a and b). Damage to the structure of the blood–testis barrier can be observed in T3 (b), empty spaces between Sertoli cells and spermatids were clearly visible after propolis exposure (c and d). Residual bodies in the cytoplasm of Sertoli cells (d). Spermatozoa with accumulated cytoplasm (d) and head and tail abnormalities (d). Barr = 10 μm (a and d), 5 μm (b, c, and e), 2 μm (f). SC: Sertoli cell; bl: Basal lamina; ld: Lipid droplets; ER: Endoplasmic reticulum swelling; dm: Dense materials; BTB: Blood–testis barrier; rb: Residual bodies; ac: Accumulated cytoplasm

Techniques Used: Transmission Assay, Electron Microscopy

25) Product Images from "Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection"

Article Title: Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection

Journal: PLoS Neglected Tropical Diseases

doi: 10.1371/journal.pntd.0000802

Infection of R06E cells with MARV and ZEBOV. (A) VeroE6 or R06E cells were infected with MARV or ZEBOV with 0.5 TCID 50 /cell. Supernatants were collected at 1, 2, 3 and 7 days p.i. and used for TCID 50 assays. (B) Supernatant from day 7 was concentrated via ultracentrifugation and viral particles were analyzed by Coomassie staining for protein composition. (C) R06E cells were infected with MARV and ZEBOV at a high MOI, fixed and inactivated at day 3 p.i.. Cells were dehydrated and embedded in Epon prior to ultrathin sectioning. Analysis by transmission electron microscopy showed viral inclusions in the perinuclear region (1, 2) and mature viral particles (3, 4). MARV and ZEBOV particles were purified via ultracentrifugation through a 20% sucrose cushion, fixed with 4% paraformaldehyde, negatively stained and analyzed by electron microscopy (5, 6). (D) VeroE6 and R06E cells were infected with MARV and ZEBOV at a high MOI, harvested at 48 h p.i. and treated as described under B. Analysis by transmission electron microscopy showed viral inclusions (broken lines) in the perinuclear region of VeroE6 (1, 2) and R06E (3, 4) cells at low magnification. Higher magnification pictures of viral inclusions in R06E cells are shown under 5 and 6.
Figure Legend Snippet: Infection of R06E cells with MARV and ZEBOV. (A) VeroE6 or R06E cells were infected with MARV or ZEBOV with 0.5 TCID 50 /cell. Supernatants were collected at 1, 2, 3 and 7 days p.i. and used for TCID 50 assays. (B) Supernatant from day 7 was concentrated via ultracentrifugation and viral particles were analyzed by Coomassie staining for protein composition. (C) R06E cells were infected with MARV and ZEBOV at a high MOI, fixed and inactivated at day 3 p.i.. Cells were dehydrated and embedded in Epon prior to ultrathin sectioning. Analysis by transmission electron microscopy showed viral inclusions in the perinuclear region (1, 2) and mature viral particles (3, 4). MARV and ZEBOV particles were purified via ultracentrifugation through a 20% sucrose cushion, fixed with 4% paraformaldehyde, negatively stained and analyzed by electron microscopy (5, 6). (D) VeroE6 and R06E cells were infected with MARV and ZEBOV at a high MOI, harvested at 48 h p.i. and treated as described under B. Analysis by transmission electron microscopy showed viral inclusions (broken lines) in the perinuclear region of VeroE6 (1, 2) and R06E (3, 4) cells at low magnification. Higher magnification pictures of viral inclusions in R06E cells are shown under 5 and 6.

Techniques Used: Infection, Staining, Transmission Assay, Electron Microscopy, Purification

26) Product Images from "Maternal exposure to diluted diesel engine exhaust alters placental function and induces intergenerational effects in rabbits"

Article Title: Maternal exposure to diluted diesel engine exhaust alters placental function and induces intergenerational effects in rabbits

Journal: Particle and Fibre Toxicology

doi: 10.1186/s12989-016-0151-7

Localization of nanoparticles in the placenta at 28 dpc. Ultrathin sections were performed on labyrinthine area in placentas from control ( a , e , i , m ) and exposed ( b - d , f - h , j - l , n - p ) dams and analyzed by TEM. Arrows indicate nanoparticles and arrowheads “finger-print” like particles. Several observations were made with various magnifications allowing the observations of different cellular compartments. Scale bars: a : 2 μm; b : 800 nm; c : 2 μm; d : 100 nm; e : 1.6 μm; f : 700 nm; g : 1.25 μm; h : 1 μm; i : 1.6 μm; j : 1 μm; k : 1 μm; l : 700 nm; m : 2.5 μm; n : 1 μm; o : 400 nm; p : 20 nm. Abbreviations: E: Erythrocyte; EC: Endothelial Cell; FV: Fetal Vessel; G: Golgi apparatus; Ly: Lysosome; m: mitochondria; MBS: Maternal Blood Space; mvb: multivesicular body; N: Nucleus; rer: rough endoplasmic reticulum; T: Trophoblast
Figure Legend Snippet: Localization of nanoparticles in the placenta at 28 dpc. Ultrathin sections were performed on labyrinthine area in placentas from control ( a , e , i , m ) and exposed ( b - d , f - h , j - l , n - p ) dams and analyzed by TEM. Arrows indicate nanoparticles and arrowheads “finger-print” like particles. Several observations were made with various magnifications allowing the observations of different cellular compartments. Scale bars: a : 2 μm; b : 800 nm; c : 2 μm; d : 100 nm; e : 1.6 μm; f : 700 nm; g : 1.25 μm; h : 1 μm; i : 1.6 μm; j : 1 μm; k : 1 μm; l : 700 nm; m : 2.5 μm; n : 1 μm; o : 400 nm; p : 20 nm. Abbreviations: E: Erythrocyte; EC: Endothelial Cell; FV: Fetal Vessel; G: Golgi apparatus; Ly: Lysosome; m: mitochondria; MBS: Maternal Blood Space; mvb: multivesicular body; N: Nucleus; rer: rough endoplasmic reticulum; T: Trophoblast

Techniques Used: Transmission Electron Microscopy

27) Product Images from "An Attenuated Strain of the Facultative Intracellular Bacterium Francisella tularensis Can Escape the Phagosome of Monocytic Cells"

Article Title: An Attenuated Strain of the Facultative Intracellular Bacterium Francisella tularensis Can Escape the Phagosome of Monocytic Cells

Journal: Infection and Immunity

doi: 10.1128/IAI.71.10.5940-5950.2003

Immunoelectron microscopic localization of the anti-FT-reactive material in extracellular F. tularensis LVS. (A) The electron-dense peroxidase reaction product stains a thick surface layer (arrowheads) of extracellular F. tularensis LVS cells. (B) No bacterial surface staining was observed when anti-FT serum was replaced with normal rabbit serum (arrowheads). Magnification, ×32,000. The ultrathin sections were examined without any additional staining.
Figure Legend Snippet: Immunoelectron microscopic localization of the anti-FT-reactive material in extracellular F. tularensis LVS. (A) The electron-dense peroxidase reaction product stains a thick surface layer (arrowheads) of extracellular F. tularensis LVS cells. (B) No bacterial surface staining was observed when anti-FT serum was replaced with normal rabbit serum (arrowheads). Magnification, ×32,000. The ultrathin sections were examined without any additional staining.

Techniques Used: Staining

28) Product Images from "Na,K-ATPase Subunit β1 knock-in Prevents Lethality of β2 Deficiency in Mice"

Article Title: Na,K-ATPase Subunit β1 knock-in Prevents Lethality of β2 Deficiency in Mice

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.18-22-09192.1998

Electron microscopic analysis of photoreceptor cells of β2/β1 +/+ and β2/β1 ki/ki mice. Ultrathin sections through retinae of 4-month-old β2/β1 +/+ ( a ) and β2/β1 ki/ki ( b ) mice. Note that the length
Figure Legend Snippet: Electron microscopic analysis of photoreceptor cells of β2/β1 +/+ and β2/β1 ki/ki mice. Ultrathin sections through retinae of 4-month-old β2/β1 +/+ ( a ) and β2/β1 ki/ki ( b ) mice. Note that the length

Techniques Used: Mouse Assay

29) Product Images from "Colocalization of integrin receptors and reelin in dendritic spine postsynaptic densities of adult nonhuman primate cortex"

Article Title: Colocalization of integrin receptors and reelin in dendritic spine postsynaptic densities of adult nonhuman primate cortex

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

Electron micrographs of ultrathin sections of Reln immunolabeling in the patas PFC layers I and II. ( A ) Immunoperoxidase reaction product in a dendritic spine is concentrated in the area of the postsynaptic density as well as the surrounding dendritic plasma membrane. ( B ) Double-immunolabeling showing the colocalization of Reln (immunoperoxidase-diaminobenzidine) and integrin α 3 -subunit (silver-enhanced 1-nm ImmunoGold) in ultrathin sections from patas PFC layers I and II. Both dendritic processes present in this electron micrograph are immunopositive for Reln and integrin α 3 -subunit. ( C ) Higher magnification of the area outlined in B . Pre, presynaptic bouton with synaptic vesicles; Post, postsynaptic spine; Den, dendritic process. (Magnification: A = ×75,000; B = ×125,000; C = ×212,500.)
Figure Legend Snippet: Electron micrographs of ultrathin sections of Reln immunolabeling in the patas PFC layers I and II. ( A ) Immunoperoxidase reaction product in a dendritic spine is concentrated in the area of the postsynaptic density as well as the surrounding dendritic plasma membrane. ( B ) Double-immunolabeling showing the colocalization of Reln (immunoperoxidase-diaminobenzidine) and integrin α 3 -subunit (silver-enhanced 1-nm ImmunoGold) in ultrathin sections from patas PFC layers I and II. Both dendritic processes present in this electron micrograph are immunopositive for Reln and integrin α 3 -subunit. ( C ) Higher magnification of the area outlined in B . Pre, presynaptic bouton with synaptic vesicles; Post, postsynaptic spine; Den, dendritic process. (Magnification: A = ×75,000; B = ×125,000; C = ×212,500.)

Techniques Used: Immunolabeling

Electron micrographs of ultrathin sections taken through the superficial layers of patas ( A ) and rat ( B ) PFC, immunostained for Reln. In both patas and rat, the immunoperoxidase reaction product in dendritic spines is concentrated in the area of the postsynaptic densities. (Magnification, ×50,000.)
Figure Legend Snippet: Electron micrographs of ultrathin sections taken through the superficial layers of patas ( A ) and rat ( B ) PFC, immunostained for Reln. In both patas and rat, the immunoperoxidase reaction product in dendritic spines is concentrated in the area of the postsynaptic densities. (Magnification, ×50,000.)

Techniques Used:

30) Product Images from "The Maize Transcription Factor Myb-Related Protein-1 Is a Key Regulator of the Differentiation of Transfer Cells [C]The Maize Transcription Factor Myb-Related Protein-1 Is a Key Regulator of the Differentiation of Transfer Cells [C] [W]"

Article Title: The Maize Transcription Factor Myb-Related Protein-1 Is a Key Regulator of the Differentiation of Transfer Cells [C]The Maize Transcription Factor Myb-Related Protein-1 Is a Key Regulator of the Differentiation of Transfer Cells [C] [W]

Journal: The Plant Cell

doi: 10.1105/tpc.108.065409

The Ectopic Expression of MRP-1 Induces TC-Like Modifications. (A) to (C) Images of the abgerminal side of nontransgenic endosperms at 12 (A) or 10 ( [B] and [C] ) DAP. (D) to (I) Images of the abgerminal side of transgenic endosperms at 12 (D) or 10 DAP ( [E] to [I] ). (Enclosed in a common frame.) (J) to (N) Images of the BETL at 12 (J) or 10 DAP ( [K] to [N] ). (A) , (D) , and (J) Wax-embedded material sectioned at 8 μm and stained with calcofluor white. CWIs appear light blue with calcofluor white staining (some indicated by arrowheads in [D] ). Transformed cells (those containing CWIs) appear in (D) , elongated along the germinal-abgerminal axis; compare these with the cubic cells at the aleurone layer (Al) or the rounded starchy endosperm cells (SE). The germinal-abgerminal (G-AB) or apico-basal (AP-B) polarity is indicated for figures (A) , (D) , and (J) . The image in (J) has been rotated 90° for comparison with the EETL. However, note that the images in (K) and (L) are shown in a natural orientation, with the placento-chalaza at the bottom part of the micrograph. (B) , (E) , (K) , and (L) Semithin sections (0.5 μm) of LR white–embedded material (10 DAP) stained with toluidine blue. Cell wall secondary growth is stained pale blue in the transformed cells (E) and TCs ( [K] and [L] ). (C) , (F) to (I) , (M) , and (N) Ultrathin sections (0.05 μm) of LR white–embedded material stained with lead nitrate and uranyl acetate. Images were taken using TEM. The corresponding areas of the cells shown in these images are framed in red in (B) , (E) , and (L) . P, pericarp side; PCH, placento-chalaza; CW, cell wall; M, mitochondria, SM, synthesis machinery associated with the internal side of the CWI. Bars = 50 μm in (A) , (D) , and (J) , 25 μm in (B) , (E) , (K) , and (L) , and the indicated values in the TEM images. Materials analyzed in (A) , (D) , and (J) were derived from the transgenic line EER-3b. Materials analyzed in (B) , (C) , (E) to (I) , and (K) to (N) were derived from the transgenic line EER-2a.
Figure Legend Snippet: The Ectopic Expression of MRP-1 Induces TC-Like Modifications. (A) to (C) Images of the abgerminal side of nontransgenic endosperms at 12 (A) or 10 ( [B] and [C] ) DAP. (D) to (I) Images of the abgerminal side of transgenic endosperms at 12 (D) or 10 DAP ( [E] to [I] ). (Enclosed in a common frame.) (J) to (N) Images of the BETL at 12 (J) or 10 DAP ( [K] to [N] ). (A) , (D) , and (J) Wax-embedded material sectioned at 8 μm and stained with calcofluor white. CWIs appear light blue with calcofluor white staining (some indicated by arrowheads in [D] ). Transformed cells (those containing CWIs) appear in (D) , elongated along the germinal-abgerminal axis; compare these with the cubic cells at the aleurone layer (Al) or the rounded starchy endosperm cells (SE). The germinal-abgerminal (G-AB) or apico-basal (AP-B) polarity is indicated for figures (A) , (D) , and (J) . The image in (J) has been rotated 90° for comparison with the EETL. However, note that the images in (K) and (L) are shown in a natural orientation, with the placento-chalaza at the bottom part of the micrograph. (B) , (E) , (K) , and (L) Semithin sections (0.5 μm) of LR white–embedded material (10 DAP) stained with toluidine blue. Cell wall secondary growth is stained pale blue in the transformed cells (E) and TCs ( [K] and [L] ). (C) , (F) to (I) , (M) , and (N) Ultrathin sections (0.05 μm) of LR white–embedded material stained with lead nitrate and uranyl acetate. Images were taken using TEM. The corresponding areas of the cells shown in these images are framed in red in (B) , (E) , and (L) . P, pericarp side; PCH, placento-chalaza; CW, cell wall; M, mitochondria, SM, synthesis machinery associated with the internal side of the CWI. Bars = 50 μm in (A) , (D) , and (J) , 25 μm in (B) , (E) , (K) , and (L) , and the indicated values in the TEM images. Materials analyzed in (A) , (D) , and (J) were derived from the transgenic line EER-3b. Materials analyzed in (B) , (C) , (E) to (I) , and (K) to (N) were derived from the transgenic line EER-2a.

Techniques Used: Expressing, Transgenic Assay, Staining, Transformation Assay, Transmission Electron Microscopy, Derivative Assay

31) Product Images from "Cold Adapted Nitrosospira sp.: A Potential Crucial Contributor of Ammonia Oxidation in Cryosols of Permafrost-Affected Landscapes in Northeast Siberia"

Article Title: Cold Adapted Nitrosospira sp.: A Potential Crucial Contributor of Ammonia Oxidation in Cryosols of Permafrost-Affected Landscapes in Northeast Siberia

Journal: Microorganisms

doi: 10.3390/microorganisms7120699

Electron microscope pictures of enrichment cultures of ammonia and nitrite-oxidizing bacteria (AOB and NOB) from soils from Samoylov Island. ( A , B ) Visualization of total cells. ( C – F ) Electron micrographs of ultrathin sections. (A–C) Nitrifying enrichment cultures derived from soils of Samoylov cliff (3306 A + B; 3304 C) with Nitrosospira -like and Nitrotoga -like cells. (D,E) Soils of polygonal tundra, rim (W17–22 cm) and slope (12–17 cm), Nitrosospira and Nitrospira microcolonies. (F) Beach soils (B0–5) with Nitrosospira -like and Nitrotoga -like cells. Bars = 0.2 µm, NSS: AOB Nitrosospira , NS: NOB Nitrospira , NT: NOB Nitrotoga , A : negatively stained image, B : scanning electron microscopic (SEM) image, C–F : transmission electron microscopic (TEM) images.
Figure Legend Snippet: Electron microscope pictures of enrichment cultures of ammonia and nitrite-oxidizing bacteria (AOB and NOB) from soils from Samoylov Island. ( A , B ) Visualization of total cells. ( C – F ) Electron micrographs of ultrathin sections. (A–C) Nitrifying enrichment cultures derived from soils of Samoylov cliff (3306 A + B; 3304 C) with Nitrosospira -like and Nitrotoga -like cells. (D,E) Soils of polygonal tundra, rim (W17–22 cm) and slope (12–17 cm), Nitrosospira and Nitrospira microcolonies. (F) Beach soils (B0–5) with Nitrosospira -like and Nitrotoga -like cells. Bars = 0.2 µm, NSS: AOB Nitrosospira , NS: NOB Nitrospira , NT: NOB Nitrotoga , A : negatively stained image, B : scanning electron microscopic (SEM) image, C–F : transmission electron microscopic (TEM) images.

Techniques Used: Microscopy, Derivative Assay, Staining, Transmission Assay, Transmission Electron Microscopy

32) Product Images from "Antiproliferative, Ultrastructural, and Physiological Effects of Amiodarone on Promastigote and Amastigote Forms of Leishmania amazonensis"

Article Title: Antiproliferative, Ultrastructural, and Physiological Effects of Amiodarone on Promastigote and Amastigote Forms of Leishmania amazonensis

Journal: Molecular Biology International

doi: 10.4061/2011/876021

Different ultrastructural alterations on Leishmania amazonensis promastigotes induced by the treatment with amiodarone (AMIO). (a) Ultrathin section of L. amazonensis promastigotes without treatment, which presents a normal ultrastructure of organelles such as (mitochondrion) m, (kinetoplast) k, (nucleus) N and (flagellum) f. (b) Electron micrograph of L. amazonensis treated with 5 μ M AMIO for 48 h presenting many vacuoles similar to autophagosomes (stars). (c–e) After treatment with 15 μ M AMIO for 24 h, it is possible to observe the presence of large autophagosomes a associated with endoplasmic reticulum profiles (big arrow), lipid bodies (arrowheads), and alterations in the mitochondrion–kinetoplast complex and chromatin condensation. (f) Promastigotes treated with 20 μ M AMIO for 24 h presented drastic alterations and destruction of the cytoplasm, where it is possible to observe the presence of autophagosomes (arrows) sometimes associated with endoplasmic reticulum profile (big arrow). A: autophagosome; f: flagellum; k: kinetoplast; m: mitochondrion; N: nucleus.
Figure Legend Snippet: Different ultrastructural alterations on Leishmania amazonensis promastigotes induced by the treatment with amiodarone (AMIO). (a) Ultrathin section of L. amazonensis promastigotes without treatment, which presents a normal ultrastructure of organelles such as (mitochondrion) m, (kinetoplast) k, (nucleus) N and (flagellum) f. (b) Electron micrograph of L. amazonensis treated with 5 μ M AMIO for 48 h presenting many vacuoles similar to autophagosomes (stars). (c–e) After treatment with 15 μ M AMIO for 24 h, it is possible to observe the presence of large autophagosomes a associated with endoplasmic reticulum profiles (big arrow), lipid bodies (arrowheads), and alterations in the mitochondrion–kinetoplast complex and chromatin condensation. (f) Promastigotes treated with 20 μ M AMIO for 24 h presented drastic alterations and destruction of the cytoplasm, where it is possible to observe the presence of autophagosomes (arrows) sometimes associated with endoplasmic reticulum profile (big arrow). A: autophagosome; f: flagellum; k: kinetoplast; m: mitochondrion; N: nucleus.

Techniques Used:

Ultrathin sections of L. amazonensis promastigotes treated with different concentrations of AMIO showing several alterations in the mitochondrial ultrastructure such as marked swelling (a, c, d, and e) with loss of the matrix content (a), alterations in the mitochondrial membrane (arrowheads), and presence of autophagic structures near a modified mitochondrion (b). A: autophagic structure; F: flagellum; k: kinetoplast; M: mitochondrion, N: nucleus.
Figure Legend Snippet: Ultrathin sections of L. amazonensis promastigotes treated with different concentrations of AMIO showing several alterations in the mitochondrial ultrastructure such as marked swelling (a, c, d, and e) with loss of the matrix content (a), alterations in the mitochondrial membrane (arrowheads), and presence of autophagic structures near a modified mitochondrion (b). A: autophagic structure; F: flagellum; k: kinetoplast; M: mitochondrion, N: nucleus.

Techniques Used: Modification

33) Product Images from "Glial Cells in the Fish Retinal Nerve Fiber Layer Form Tight Junctions, Separating and Surrounding Axons"

Article Title: Glial Cells in the Fish Retinal Nerve Fiber Layer Form Tight Junctions, Separating and Surrounding Axons

Journal: Frontiers in Molecular Neuroscience

doi: 10.3389/fnmol.2018.00367

Tight junctions in loose wrap myelin in freeze fracture and ultrathin section electron micrographs of the retinal NFL. Tight junction strands (arrows) do not run parallel to the axon in varying distances and patterns, and between wrapping layers (a,b) . Tight junctional contacts between lamellae could be verified in sections (c) . The top panel shows overviews, the square frames indicate areas shown at higher magnifications in the bottom panel. V, vitreous humor; MC, Müller cell endfeet; Ax, Axon. Scale bars as indicated.
Figure Legend Snippet: Tight junctions in loose wrap myelin in freeze fracture and ultrathin section electron micrographs of the retinal NFL. Tight junction strands (arrows) do not run parallel to the axon in varying distances and patterns, and between wrapping layers (a,b) . Tight junctional contacts between lamellae could be verified in sections (c) . The top panel shows overviews, the square frames indicate areas shown at higher magnifications in the bottom panel. V, vitreous humor; MC, Müller cell endfeet; Ax, Axon. Scale bars as indicated.

Techniques Used:

34) Product Images from "Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons"

Article Title: Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons

Journal: eLife

doi: 10.7554/eLife.28360

Transmission electron microscopy of ChO in control and dCirl KO . Electron micrographs of longitudinal ultrathin sections at the distal dendritic region of the two outer (1, 2; 1’, 2’) scolopidia document a complex organization. In scolopidia 1, 1’ and 2’, the section passes through the central dendritic region including ciliary rootlets, ciliary origin with basal bodies and the entire (1) or part of (1’, 2’) the cilium; scolopidium 2 is sectioned peripherally. General cellular architecture and ultrastructural features appear preserved in these distal scolopidia after dCirl removal. Scale bar, 1 µm. DOI: http://dx.doi.org/10.7554/eLife.28360.004
Figure Legend Snippet: Transmission electron microscopy of ChO in control and dCirl KO . Electron micrographs of longitudinal ultrathin sections at the distal dendritic region of the two outer (1, 2; 1’, 2’) scolopidia document a complex organization. In scolopidia 1, 1’ and 2’, the section passes through the central dendritic region including ciliary rootlets, ciliary origin with basal bodies and the entire (1) or part of (1’, 2’) the cilium; scolopidium 2 is sectioned peripherally. General cellular architecture and ultrastructural features appear preserved in these distal scolopidia after dCirl removal. Scale bar, 1 µm. DOI: http://dx.doi.org/10.7554/eLife.28360.004

Techniques Used: Transmission Assay, Electron Microscopy

35) Product Images from "Neurodegeneration with Tau Accumulation in a Transgenic Mouse Expressing V337M Human Tau"

Article Title: Neurodegeneration with Tau Accumulation in a Transgenic Mouse Expressing V337M Human Tau

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.22-01-00133.2002

Ultrastructural analysis of irregularly shaped neurons. a , Light photomicrograph of a toluidine blue-stained ultrathin section from the hippocampus of a Tg mouse, showing irregularly shaped neurons. b , Low-power electron photomicrograph of hippocampal neurons from a Tg214 mouse, showing an example in the center of dark cell degeneration. c , Examination of this electron-dense cell at a higher magnification clearly shows the accumulation of ribosomes ( Rb ) and lipofuscin ( Lp ). d , The presence of bundles of straight tubules in the cytoplasm of this neuron is also evident at higher magnifications. Further examination of these tubules at a higher magnification ( f ) confirms that the diameter of these bundles is ∼15 nm. e , Higher magnification reveals the swelling of Golgi network ( GN ) and the ruffling of nuclear ( Nu ) membrane. Scale bars: a , 60 μm; b , 5 μm; c , 350 nm; d , 1 μm; e , 160 nm; f , 100 nm.
Figure Legend Snippet: Ultrastructural analysis of irregularly shaped neurons. a , Light photomicrograph of a toluidine blue-stained ultrathin section from the hippocampus of a Tg mouse, showing irregularly shaped neurons. b , Low-power electron photomicrograph of hippocampal neurons from a Tg214 mouse, showing an example in the center of dark cell degeneration. c , Examination of this electron-dense cell at a higher magnification clearly shows the accumulation of ribosomes ( Rb ) and lipofuscin ( Lp ). d , The presence of bundles of straight tubules in the cytoplasm of this neuron is also evident at higher magnifications. Further examination of these tubules at a higher magnification ( f ) confirms that the diameter of these bundles is ∼15 nm. e , Higher magnification reveals the swelling of Golgi network ( GN ) and the ruffling of nuclear ( Nu ) membrane. Scale bars: a , 60 μm; b , 5 μm; c , 350 nm; d , 1 μm; e , 160 nm; f , 100 nm.

Techniques Used: Staining

36) Product Images from "Psychological stress downregulates epidermal antimicrobial peptide expression and increases severity of cutaneous infections in mice"

Article Title: Psychological stress downregulates epidermal antimicrobial peptide expression and increases severity of cutaneous infections in mice

Journal: The Journal of Clinical Investigation

doi: 10.1172/JCI31726

PS-induced decrease in AMP delivery to epidermal LBs is reversed by RU-486. ( A and B ) Ultrathin sections labeled with CRAMP ( A ) and mBD3 ( B ) primary antibodies followed by a 10-nm colloidal gold–tagged secondary antibody after embedding for electron microscopy. Sections were postfixed in osmium tetroxide and embedded in LR White medium. Black arrows denote unlabeled LBs; circles indicate label in cytosol. ( A ) CRAMP was labeled (black arrowheads) in nonstressed normal controls (Co), and CRAMP labeling reappeared in PS mice treated with RU-486 (PS+Ru), but not with exogenous lipids (PS+L). ( B ) Exogenous lipids restored labeling of mBD3 in LB in PS mice (black arrowheads). Scale bars: 100 nm. ( C – F ) Quantitative data for immunolabeling of CRAMP and mBD3 in LB in nonstressed control or PS mice plus either RU-486 ( C and D ) or lipid ( E and F ) cotreatment. * P
Figure Legend Snippet: PS-induced decrease in AMP delivery to epidermal LBs is reversed by RU-486. ( A and B ) Ultrathin sections labeled with CRAMP ( A ) and mBD3 ( B ) primary antibodies followed by a 10-nm colloidal gold–tagged secondary antibody after embedding for electron microscopy. Sections were postfixed in osmium tetroxide and embedded in LR White medium. Black arrows denote unlabeled LBs; circles indicate label in cytosol. ( A ) CRAMP was labeled (black arrowheads) in nonstressed normal controls (Co), and CRAMP labeling reappeared in PS mice treated with RU-486 (PS+Ru), but not with exogenous lipids (PS+L). ( B ) Exogenous lipids restored labeling of mBD3 in LB in PS mice (black arrowheads). Scale bars: 100 nm. ( C – F ) Quantitative data for immunolabeling of CRAMP and mBD3 in LB in nonstressed control or PS mice plus either RU-486 ( C and D ) or lipid ( E and F ) cotreatment. * P

Techniques Used: Labeling, Electron Microscopy, Mouse Assay, Immunolabeling

37) Product Images from "An alternative pathway for rod signals in the rodent retina: Rod photoreceptors, cone bipolar cells, and the localization of glutamate receptors"

Article Title: An alternative pathway for rod signals in the rodent retina: Rod photoreceptors, cone bipolar cells, and the localization of glutamate receptors

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

( A–H ) Electron micrographs showing a series of ultrathin sections taken through a flat noninvaginating synaptic contact made by a putative OFF-cone bipolar cell dendrite labeled for GluR2 at a rod spherule in the OPL of rat. The presynaptic ribbon in the rod spherule is marked by an arrowhead. Bar = 0.2 μm.
Figure Legend Snippet: ( A–H ) Electron micrographs showing a series of ultrathin sections taken through a flat noninvaginating synaptic contact made by a putative OFF-cone bipolar cell dendrite labeled for GluR2 at a rod spherule in the OPL of rat. The presynaptic ribbon in the rod spherule is marked by an arrowhead. Bar = 0.2 μm.

Techniques Used: Labeling

38) Product Images from "Group I Metabotropic Glutamate Receptors in the Monkey Striatum: Subsynaptic Association with Glutamatergic and Dopaminergic Afferents"

Article Title: Group I Metabotropic Glutamate Receptors in the Monkey Striatum: Subsynaptic Association with Glutamatergic and Dopaminergic Afferents

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.23-20-07659.2003

Serial ultrathin sections through perisynaptic mGluR5 immunoreactivity (arrows) at an asymmetric axospinous synapse (arrowheads) established by an anterogradely labeled cortical bouton. Note the high degree of specificity of the perisynaptic immunogold labeling in these sections. Sp, Dendritic spines. Scale bar: (in A ), A-D , 0.25 μm.
Figure Legend Snippet: Serial ultrathin sections through perisynaptic mGluR5 immunoreactivity (arrows) at an asymmetric axospinous synapse (arrowheads) established by an anterogradely labeled cortical bouton. Note the high degree of specificity of the perisynaptic immunogold labeling in these sections. Sp, Dendritic spines. Scale bar: (in A ), A-D , 0.25 μm.

Techniques Used: Labeling

39) Product Images from "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting"

Article Title: Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting

Journal: The EMBO Journal

doi: 10.1002/embj.201386120

FXYD6 and Tpd52 are localized to VGLUT1-positive synapses. A Regional distribution of FXYD6 in adult mouse brain homogenates. ob, olfactory bulb; ctx, cerebral cortex; cp, caudate-putamen; hc, hippocampus; th, thalamus; hy, hypothalamus; cb, cerebellum; bs, brainstem. B FXYD6, VGLUT1, GluN1 distribution in subcellular fractions of mouse brain. Note that FXYD6 is markedly enriched in the synaptic plasma membrane fraction LP1B. H,homogenate; P1, nuclear pellet; S1, supernatant 1; P2, crude synaptosomes; S2, supernatant 2; P3, microsomal fraction; S3, somatic soluble fraction; LP1, lysed P2 pellet 1; LP1A, myelin-rich light membrane fraction; LP1B, synaptic plasma membrane fraction; LP2, crude SVs; LS2, soluble synaptic fraction. C FXYD6 enrichment in sorted VGLUT1 VENUS synaptosomes by Western blot analysis. The average CF of 0.97 for FXYD6 with VGLUT1 in FASS-purified synaptosomes indicates a high rate of copurification of the two markers. The error bar indicates the s.e.m. n , independent experiments. D Immunofluorescence of FXYD6 (red), VGLUT1 (green), and PSD95 (blue) in primary cultured hippocampal neurons. Scale bars, 2 μm in overviews and 0.4 μm for enlarged images. E, F Pre-embedding immunoelectron microscopy of ultrathin sections of mouse hippocampus using the anti-FXYD6 antibody. HPC, hippocampus; t, terminal; a, axon; d, dendrite. Double arrows mark synaptic contacts. Scale bars, 0.5 μm (E and F). G Western blot analysis of the distribution of Tpd52 in homogenates of different brain regions of adult mice. ob, olfactory bulb; ctx, cerebral cortex; cp, caudate-putamen; hc, hippocampus; th/hy, thalamus and hypothalamus; mb, mid brain including colliculi, substantia nigra and ventral tegmental area; cb, cerebellum, bs, brainstem; sc, spinal cord. H Western blot analysis of Tpd52 in subcellular fractions of mouse brain. Samples used here are identical to those employed in (B). I Representative immunofluorescence micrographs of sorted VGLUT1 VENUS -positive synaptosomes stained for VGLUT1 VENUS (anti-GFP) and Tpd52. Scale bar, 1 μm. J Quantification of VGLUT1 VENUS /Tpd52 double positive particles (see C). A total of 168 GFP-positive particles from one FASS experiment were analyzed ( n = 168; N = 1). K Triple immunofluorescence staining for VGLUT1 (green), Tpd52 (red), and PSD95 (blue) in cultured primary hippocampal neurons (DIV22). Arrowheads mark examples of co-localization of Tpd52 with VGLUT1 or PSD95. Scale bar, 1 μm in enlarged images and 6 μm for overviews.
Figure Legend Snippet: FXYD6 and Tpd52 are localized to VGLUT1-positive synapses. A Regional distribution of FXYD6 in adult mouse brain homogenates. ob, olfactory bulb; ctx, cerebral cortex; cp, caudate-putamen; hc, hippocampus; th, thalamus; hy, hypothalamus; cb, cerebellum; bs, brainstem. B FXYD6, VGLUT1, GluN1 distribution in subcellular fractions of mouse brain. Note that FXYD6 is markedly enriched in the synaptic plasma membrane fraction LP1B. H,homogenate; P1, nuclear pellet; S1, supernatant 1; P2, crude synaptosomes; S2, supernatant 2; P3, microsomal fraction; S3, somatic soluble fraction; LP1, lysed P2 pellet 1; LP1A, myelin-rich light membrane fraction; LP1B, synaptic plasma membrane fraction; LP2, crude SVs; LS2, soluble synaptic fraction. C FXYD6 enrichment in sorted VGLUT1 VENUS synaptosomes by Western blot analysis. The average CF of 0.97 for FXYD6 with VGLUT1 in FASS-purified synaptosomes indicates a high rate of copurification of the two markers. The error bar indicates the s.e.m. n , independent experiments. D Immunofluorescence of FXYD6 (red), VGLUT1 (green), and PSD95 (blue) in primary cultured hippocampal neurons. Scale bars, 2 μm in overviews and 0.4 μm for enlarged images. E, F Pre-embedding immunoelectron microscopy of ultrathin sections of mouse hippocampus using the anti-FXYD6 antibody. HPC, hippocampus; t, terminal; a, axon; d, dendrite. Double arrows mark synaptic contacts. Scale bars, 0.5 μm (E and F). G Western blot analysis of the distribution of Tpd52 in homogenates of different brain regions of adult mice. ob, olfactory bulb; ctx, cerebral cortex; cp, caudate-putamen; hc, hippocampus; th/hy, thalamus and hypothalamus; mb, mid brain including colliculi, substantia nigra and ventral tegmental area; cb, cerebellum, bs, brainstem; sc, spinal cord. H Western blot analysis of Tpd52 in subcellular fractions of mouse brain. Samples used here are identical to those employed in (B). I Representative immunofluorescence micrographs of sorted VGLUT1 VENUS -positive synaptosomes stained for VGLUT1 VENUS (anti-GFP) and Tpd52. Scale bar, 1 μm. J Quantification of VGLUT1 VENUS /Tpd52 double positive particles (see C). A total of 168 GFP-positive particles from one FASS experiment were analyzed ( n = 168; N = 1). K Triple immunofluorescence staining for VGLUT1 (green), Tpd52 (red), and PSD95 (blue) in cultured primary hippocampal neurons (DIV22). Arrowheads mark examples of co-localization of Tpd52 with VGLUT1 or PSD95. Scale bar, 1 μm in enlarged images and 6 μm for overviews.

Techniques Used: Western Blot, Purification, Copurification, Immunofluorescence, Cell Culture, Immuno-Electron Microscopy, Mouse Assay, Staining

40) Product Images from "Glucose Transporter 1 and Monocarboxylate Transporters 1, 2, and 4 Localization within the Glial Cells of Shark Blood-Brain-Barriers"

Article Title: Glucose Transporter 1 and Monocarboxylate Transporters 1, 2, and 4 Localization within the Glial Cells of Shark Blood-Brain-Barriers

Journal: PLoS ONE

doi: 10.1371/journal.pone.0032409

Three-dimensional reconstruction of glial end-feet in the brain cortex. A, Ultrastructural images from shark brain ( S. chilensis ) using low magnification. The telencephalic neurophil showed neural processes and glial cell end-feet contacting the blood vessel. B–C, Forty ultrathin sections (50 nm) were used to create a three-dimensional reconstruction of dendritic (blue) and glial end-feet (green) around a blood vessel (gray). D, Most of the blood vessel is surrounded by glial end-feet that form an irregular barrier. Scale bar: A–C, 15 µm.
Figure Legend Snippet: Three-dimensional reconstruction of glial end-feet in the brain cortex. A, Ultrastructural images from shark brain ( S. chilensis ) using low magnification. The telencephalic neurophil showed neural processes and glial cell end-feet contacting the blood vessel. B–C, Forty ultrathin sections (50 nm) were used to create a three-dimensional reconstruction of dendritic (blue) and glial end-feet (green) around a blood vessel (gray). D, Most of the blood vessel is surrounded by glial end-feet that form an irregular barrier. Scale bar: A–C, 15 µm.

Techniques Used:

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Staining:

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Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication
Article Snippet: .. Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope. .. In Situ Hybridization For in situ hybridization, we used one probe ( 5′-TGACCATCATGGACCTCCA-3′ ), which anneals in a conserved region inside the NS3 gene in the negative strand of viral RNA, and contained six dispersed locked nucleic acid modified bases with digoxigenin conjugated to the 5′ end.

Incubation:

Article Title: Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus
Article Snippet: .. Bound MAbs were detected by incubation with anti-hu-IgG-5-nm gold conjugates (British Biocell International, Cardiff, United Kingdom), and ultrathin sections were evaluated under a ZEISS EM 10A transmission electron microscope. .. Spike (S)-transfected 293T cells were incubated with human IgGs at a concentration of 10 μg/ml for 1 h on ice.

Microscopy:

Article Title: Cytopathological Effects of Bacillus sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex quinquefasciatus Larvae ▿
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with a Zeiss EM 109 transmission electron microscope. ..

Article Title: The Compensatory G88R Change Is Essential in Restoring the Normal Functions of Influenza A/WSN/33 Virus Matrix Protein 1 with a Disrupted Nuclear Localization Signal
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and were examined under a Zeiss EM 912 transmission electron microscope (TEM) equipped with a Keenview digital camera (Olympus). ..

Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication
Article Snippet: .. Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope. .. In Situ Hybridization For in situ hybridization, we used one probe ( 5′-TGACCATCATGGACCTCCA-3′ ), which anneals in a conserved region inside the NS3 gene in the negative strand of viral RNA, and contained six dispersed locked nucleic acid modified bases with digoxigenin conjugated to the 5′ end.

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Transmission Electron Microscopy:

Article Title: The Compensatory G88R Change Is Essential in Restoring the Normal Functions of Influenza A/WSN/33 Virus Matrix Protein 1 with a Disrupted Nuclear Localization Signal
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and were examined under a Zeiss EM 912 transmission electron microscope (TEM) equipped with a Keenview digital camera (Olympus). ..

Article Title: Fusion of Hematopoietic Cells with Purkinje Neurons Does Not Lead to Stable Heterokaryon Formation under Noninvasive Conditions
Article Snippet: .. Ultrathin sections (50 nm; ∼200 per LacZ-expressing cell) were serially cut on a Leica Ultracut UCT, stained with uranyl acetate and lead citrate, and analyzed using a Zeiss EM 10CR TEM. ..

Transmission Assay:

Article Title: Cytopathological Effects of Bacillus sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex quinquefasciatus Larvae ▿
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with a Zeiss EM 109 transmission electron microscope. ..

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Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication
Article Snippet: .. Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope. .. In Situ Hybridization For in situ hybridization, we used one probe ( 5′-TGACCATCATGGACCTCCA-3′ ), which anneals in a conserved region inside the NS3 gene in the negative strand of viral RNA, and contained six dispersed locked nucleic acid modified bases with digoxigenin conjugated to the 5′ end.

Article Title: Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus
Article Snippet: .. Bound MAbs were detected by incubation with anti-hu-IgG-5-nm gold conjugates (British Biocell International, Cardiff, United Kingdom), and ultrathin sections were evaluated under a ZEISS EM 10A transmission electron microscope. .. Spike (S)-transfected 293T cells were incubated with human IgGs at a concentration of 10 μg/ml for 1 h on ice.

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    Carl Zeiss ultrathin sections
    Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) <t>Ultrathin</t> section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P
    Ultrathin Sections, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 815 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Carl Zeiss em 912 transmission electron microscope tem
    Virion morphology changes and matrix protein 1 (M1) cytoplasmic disintegration under different pH treatments. ( A ) Representative transmission electron microscopy <t>(TEM)</t> images of purified wild-type A/WSN/33 (WSN), M(NLS-88R) or M(NLS-88E) pretreated with the indicated pH buffers. Images were acquired under a Zeiss <t>EM</t> 912 transmission electron microscope equipped with a Keenview digital camera. Black and tan arrows indicate M1 layer and spikes, respectively. ( B ) The percentages of partially spiked virions per ∼100 viral particles blindly counted by two individuals. The data are expressed as the average±SEM. ( C ) The average M1 thickness in different pH pretreated virions under TEM ( n =10 virions). *** P
    Em 912 Transmission Electron Microscope Tem, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) Ultrathin section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the liver. (a) Liver of a non-dengue case stained with HE and presenting normal aspect. (b–e) Liver sections of dengue cases, stained with HE, showing hepatic injuries, including micro (Mi) and macrovesicular (Ma) steatosis, necrosis (N), edema (E) and hemorrhage (He) near central vein (CV). (f) Semi-thin section of a non-dengue case presenting hepatocytes and sinusoidal capillaries with normal structures and (g) one dengue case presenting micro (Mi) and macrosteatosis (Ma), nuclear degeneration (black star) and numerous macrophage cells (Mø). (h) Ultrathin section of a non-dengue case exhibiting normal hepatocytes (H) and regular sinusoidal capillaries (SC) with the presence of monocytes (Mo) and Kupffer cells (KC) and (i and j) dengue cases showing large lipid droplets (LD) in the cytoplasm of hepatocytes, swollen mitochondria (red stars) and presence of platelet (Pt) inside sinusoidal capillaries (SC) with loss of endothelium. Semi-thin and ultrathin sections of liver were stained with methylene blue/azure II solution and uranyl acetate/lead citrate, respectively. Quantitative studies of histological damages were made individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis were performed comparing the mean values of each group (dengue patients vs non-dengue patients). Damages were quantified by the percentage of affected area for (k) hemorrhage and (l) edema or (m) by steatosis degree using a scale ranging from 0 to 4. (n–o) Steatosis was also evaluated in each hepatic acini area (periportal, midzonal and central vein) by plotting different damage degrees (ten fields for each case). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the lung. (a) Lung of a non-dengue case stained with HE and presenting normal aspect of alveoli (A) and alveolar septa (AS). (b–g) Lung sections of dengue cases, stained with HE, showing pulmonary alterations, including septal thickening (St), edema (E), hemorrhage (He), presence of mononuclear infiltrate (Inf), hyaline membrane formation (HM) and hypertrophy of alveolar macrophages (AM) and type II pneumocytes (PcyII). (h) Semi-thin section of a non-dengue case showing alveoli (A), alveolar septa (AS), endothelial cells (EC) and type I (PcyI) and II pneumocytes (PcyII) with normal aspects. (i) Semi-thin section of one dengue case presenting numerous platelets (Pt) and megakaryocytes (MK) inside alveolar septa. (j) Ultrathin section of one non-dengue case exhibiting regular alveoli, alveolar septum, type I and II pneumocytes and endothelial cell. (j-l) Ultrathin sections of dengue cases exhibited type II pneumocytes located in alveolar space in contact with numerous platelets, the appearance of hyaline membrane and the presence of virus particles (VP) in endothelium. Quantitative analysis of hemorrhage (m) and edema (n) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the lung. (a) Lung of a non-dengue case stained with HE and presenting normal aspect of alveoli (A) and alveolar septa (AS). (b–g) Lung sections of dengue cases, stained with HE, showing pulmonary alterations, including septal thickening (St), edema (E), hemorrhage (He), presence of mononuclear infiltrate (Inf), hyaline membrane formation (HM) and hypertrophy of alveolar macrophages (AM) and type II pneumocytes (PcyII). (h) Semi-thin section of a non-dengue case showing alveoli (A), alveolar septa (AS), endothelial cells (EC) and type I (PcyI) and II pneumocytes (PcyII) with normal aspects. (i) Semi-thin section of one dengue case presenting numerous platelets (Pt) and megakaryocytes (MK) inside alveolar septa. (j) Ultrathin section of one non-dengue case exhibiting regular alveoli, alveolar septum, type I and II pneumocytes and endothelial cell. (j-l) Ultrathin sections of dengue cases exhibited type II pneumocytes located in alveolar space in contact with numerous platelets, the appearance of hyaline membrane and the presence of virus particles (VP) in endothelium. Quantitative analysis of hemorrhage (m) and edema (n) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the spleen. (a) Spleen of a non-dengue case stained with HE and presenting normal aspect. (b and c) Spleen sections of dengue cases, stained with HE, showing vascular congestion (VC), edema (E) and an atrophy of lymphoid follicles. Red pulp (RP); white pulp (WP). (d) Semi-thin section of a non-dengue case revealing red pulp with regular aspect and normal splenocytes (S). (e) Semi-thin section of a dengue case showing vacuolization (V) and degenerated splenocytes (DS). (f) Ultra-thin section of a non-dengue case with regular splenocytes (S) and (g and h) dengue cases exhibiting vacuolization (V) around degenerated splenocytes and loss of the endothelium of splenic sinusoid (SS). Semi-thin and ultrathin sections were stained as described in figure 1 . (i–k) Quantitative analysis of histological damages observed individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). The media of lymphoid follicle areas were quantified (i), as well as the percentage areas with vascular congestion (j) and edema (k). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the spleen. (a) Spleen of a non-dengue case stained with HE and presenting normal aspect. (b and c) Spleen sections of dengue cases, stained with HE, showing vascular congestion (VC), edema (E) and an atrophy of lymphoid follicles. Red pulp (RP); white pulp (WP). (d) Semi-thin section of a non-dengue case revealing red pulp with regular aspect and normal splenocytes (S). (e) Semi-thin section of a dengue case showing vacuolization (V) and degenerated splenocytes (DS). (f) Ultra-thin section of a non-dengue case with regular splenocytes (S) and (g and h) dengue cases exhibiting vacuolization (V) around degenerated splenocytes and loss of the endothelium of splenic sinusoid (SS). Semi-thin and ultrathin sections were stained as described in figure 1 . (i–k) Quantitative analysis of histological damages observed individually in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). The media of lymphoid follicle areas were quantified (i), as well as the percentage areas with vascular congestion (j) and edema (k). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological and ultrastructural analysis of the heart. (a) Heart of a non-dengue case stained with H.E. and presenting normal aspect. (b and c) Heart sections of dengue cases, stained with HE, showing cardiac injuries, including hemorrhage (He), edema (E), presence of mononuclear infiltrate (Inf) and degeneration of muscle fibers (black star). (d and f) Semi-thin and ultrathin sections of a non-dengue case presenting cardiac fibers (CF) with normal nucleus (N), mitochondria (M), capillaries (Cap) and intercalated discs (ID). (e) Semi-thin section of one dengue case presenting degeneration of cardiac fibers (black star) characterized by absence of nucleus and a diffuse interstitial edema (E) and (g and h) ultrathin sections showing nuclear (white stars) and mitochondria alterations (M) in cardiomyocytes and interstitial edema. Quantitative analysis of hemorrhage (i) and edema (j) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological and ultrastructural analysis of the heart. (a) Heart of a non-dengue case stained with H.E. and presenting normal aspect. (b and c) Heart sections of dengue cases, stained with HE, showing cardiac injuries, including hemorrhage (He), edema (E), presence of mononuclear infiltrate (Inf) and degeneration of muscle fibers (black star). (d and f) Semi-thin and ultrathin sections of a non-dengue case presenting cardiac fibers (CF) with normal nucleus (N), mitochondria (M), capillaries (Cap) and intercalated discs (ID). (e) Semi-thin section of one dengue case presenting degeneration of cardiac fibers (black star) characterized by absence of nucleus and a diffuse interstitial edema (E) and (g and h) ultrathin sections showing nuclear (white stars) and mitochondria alterations (M) in cardiomyocytes and interstitial edema. Quantitative analysis of hemorrhage (i) and edema (j) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Histopathological/ultrastructural analysis and dengue detection in the kidney. (a) Kidney of a non-dengue case stained with HE and presenting normal aspect. (b and c) Kidney sections of dengue cases, stained with HE, showing injuries, including: hemorrhage (He), edema (E), sloughing of necrotic cells with loss of the basement membrane (black star), mainly in proximal convoluted tubule (PCT) but also detected in distal convoluted tubule (DCT), and areas of cellular regeneration (blue star) near renal glomerulus (RG). (d and e) Semi-thin sections of non-dengue cases showing Bowman’s capsule (BC) and podocytes (Pdc) around glomerular capillaries (GC), mensagial cells (MC) and endothelial cell (EC) with regular structures and preserved capillaries (Cap), epithelial cells (Ep) and distal and proximal convolutes tubules (DCT and PCT, respectively). (f and g) Dengue cases with the presence of thrombus (T) in capillaries of renal glomerulus and necrotic cells (NC) in the lumen of proximal convoluted tubules. (h) Ultrathin of a non-dengue case exhibiting conserved glomerular capillaries. (i) Ultrathin of one dengue case showing necrotic cell with picnotic nucleus (white star) and dilatation of rough endoplasmic reticulum (ER). Quantitative analysis of hemorrhage (j) and edema (k) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Journal: PLoS ONE

    Article Title: The Pathology of Severe Dengue in Multiple Organs of Human Fatal Cases: Histopathology, Ultrastructure and Virus Replication

    doi: 10.1371/journal.pone.0083386

    Figure Lengend Snippet: Histopathological/ultrastructural analysis and dengue detection in the kidney. (a) Kidney of a non-dengue case stained with HE and presenting normal aspect. (b and c) Kidney sections of dengue cases, stained with HE, showing injuries, including: hemorrhage (He), edema (E), sloughing of necrotic cells with loss of the basement membrane (black star), mainly in proximal convoluted tubule (PCT) but also detected in distal convoluted tubule (DCT), and areas of cellular regeneration (blue star) near renal glomerulus (RG). (d and e) Semi-thin sections of non-dengue cases showing Bowman’s capsule (BC) and podocytes (Pdc) around glomerular capillaries (GC), mensagial cells (MC) and endothelial cell (EC) with regular structures and preserved capillaries (Cap), epithelial cells (Ep) and distal and proximal convolutes tubules (DCT and PCT, respectively). (f and g) Dengue cases with the presence of thrombus (T) in capillaries of renal glomerulus and necrotic cells (NC) in the lumen of proximal convoluted tubules. (h) Ultrathin of a non-dengue case exhibiting conserved glomerular capillaries. (i) Ultrathin of one dengue case showing necrotic cell with picnotic nucleus (white star) and dilatation of rough endoplasmic reticulum (ER). Quantitative analysis of hemorrhage (j) and edema (k) observed in dengue (cases 1–4) and non-dengue patients (cont. 1–4), and statistical analysis performed comparing the mean values of each group (dengue patients vs non-dengue patients). Asterisks indicate differences that are statistically significant between control and dengue groups, (*) (P

    Article Snippet: Ultrathin sections (60–90 nm thick) were stained with uranyl acetate and lead citrate , and were observed in a Zeiss EM-900 transmission electron microscope.

    Techniques: Staining

    Electron microscopic confirmation of single nuclei in LacZ-labeled Purkinje neurons in vav-iCre/LacZ mice. a , Example of a LacZ-positive Purkinje neuron, detected by X-gal staining of a 50-μm-thick Vibratome section, located in the Purkinje neuron layer (PC), between molecular (mol) and granule (gr) cell layer. b , Larger image of the same cell as in a . c , Light microscopic staining of calbindin confirming that all large X-gal-positive cell bodies in the PC are indeed Purkinje neurons. Typical dotted X-gal stain in the cell body and dendrite indicated by black arrowheads. d , e , Serial ultrathin sections through the same PKN as shown in a and b reveal only a single nucleus. The nucleus is large and light with a dark basophilic nucleolus, with deep folds and invaginations toward the side of the dendrites and Nissl bodies (NB) at the side of the folds, thus displaying the typical morphologic features of Purkinje neurons. f , g , Same cell at different z -levels and smaller magnification showing no second nucleus in this neuron. Scale bars: a–c , 20 μm; d , e , 2 μm; f , g , 5 μm.

    Journal: The Journal of Neuroscience

    Article Title: Fusion of Hematopoietic Cells with Purkinje Neurons Does Not Lead to Stable Heterokaryon Formation under Noninvasive Conditions

    doi: 10.1523/JNEUROSCI.5848-08.2009

    Figure Lengend Snippet: Electron microscopic confirmation of single nuclei in LacZ-labeled Purkinje neurons in vav-iCre/LacZ mice. a , Example of a LacZ-positive Purkinje neuron, detected by X-gal staining of a 50-μm-thick Vibratome section, located in the Purkinje neuron layer (PC), between molecular (mol) and granule (gr) cell layer. b , Larger image of the same cell as in a . c , Light microscopic staining of calbindin confirming that all large X-gal-positive cell bodies in the PC are indeed Purkinje neurons. Typical dotted X-gal stain in the cell body and dendrite indicated by black arrowheads. d , e , Serial ultrathin sections through the same PKN as shown in a and b reveal only a single nucleus. The nucleus is large and light with a dark basophilic nucleolus, with deep folds and invaginations toward the side of the dendrites and Nissl bodies (NB) at the side of the folds, thus displaying the typical morphologic features of Purkinje neurons. f , g , Same cell at different z -levels and smaller magnification showing no second nucleus in this neuron. Scale bars: a–c , 20 μm; d , e , 2 μm; f , g , 5 μm.

    Article Snippet: Ultrathin sections (50 nm; ∼200 per LacZ-expressing cell) were serially cut on a Leica Ultracut UCT, stained with uranyl acetate and lead citrate, and analyzed using a Zeiss EM 10CR TEM.

    Techniques: Labeling, Mouse Assay, Staining

    Vesicles and synapses of neuronal profiles in the CBL. Details from frontal ultrathin sections through the CBL. (A) Central neuropil area showing neuronal profiles with different vesicle content (N1–N3) and processes from a glial cell (G). N1, neuronal profile with large granular vesicles (arrowheads); N2, neuronal profile with small clear pleomorphic vesicles and large dense core vesicles; N3, profile with circular small clear vesicles (arrows) and large ovoid dense core vesicles (white arrowheads). Note the different appearance of the small vesicles in N2 and N3. (B) Central neuropil area. Neuronal profile with large granular vesicles (N1) and two dyadic output synapses (s1, s2, arrowheads). Both synapses are of type I, i.e., one presynaptic profile (N1) faces two postsynaptic profiles (N2 and N3, resp. N3 and N4). Inset shows synaptic profiles at higher magnification (45,000×). Both synapses show a presynaptic membrane density (arrowheads) surrounded by synaptic vesicles. The intercellular space in enlarged at the active zone. The postsynaptic membrane only shows minor accumulation of electron dense material (white arrow). (C) Type II synapse. Two presynaptic profiles (N1,N2) with small clear synaptic vesicles and large granular vesicles face a postsynaptic profile with small clear and large dense core vesicles. Arrowheads point to presynaptic densities with accumulations of clear synaptic vesicles. The asterisk marks a profile with large granular vesicles. The membrane of most vesicles is broken (arrows). These have a lighter granular content than intact vesicles (white arrowheads). (D) Magnified detail from Figure 2C . Profiles N1 and N2 with small clear and dark dense core vesicles are presynaptic to a third small profile (asterisk). In adjacent sections, this profile contained large granular vesicles. Presynaptic structures consist of a bar-shaped density surrounded by small clear vesicles. The synapses may be monadic or dyadic. Scale bar in (D) : 0.5 μm (applies to A–D ).

    Journal: Frontiers in Behavioral Neuroscience

    Article Title: Ultrastructure of GABA- and Tachykinin-Immunoreactive Neurons in the Lower Division of the Central Body of the Desert Locust

    doi: 10.3389/fnbeh.2016.00230

    Figure Lengend Snippet: Vesicles and synapses of neuronal profiles in the CBL. Details from frontal ultrathin sections through the CBL. (A) Central neuropil area showing neuronal profiles with different vesicle content (N1–N3) and processes from a glial cell (G). N1, neuronal profile with large granular vesicles (arrowheads); N2, neuronal profile with small clear pleomorphic vesicles and large dense core vesicles; N3, profile with circular small clear vesicles (arrows) and large ovoid dense core vesicles (white arrowheads). Note the different appearance of the small vesicles in N2 and N3. (B) Central neuropil area. Neuronal profile with large granular vesicles (N1) and two dyadic output synapses (s1, s2, arrowheads). Both synapses are of type I, i.e., one presynaptic profile (N1) faces two postsynaptic profiles (N2 and N3, resp. N3 and N4). Inset shows synaptic profiles at higher magnification (45,000×). Both synapses show a presynaptic membrane density (arrowheads) surrounded by synaptic vesicles. The intercellular space in enlarged at the active zone. The postsynaptic membrane only shows minor accumulation of electron dense material (white arrow). (C) Type II synapse. Two presynaptic profiles (N1,N2) with small clear synaptic vesicles and large granular vesicles face a postsynaptic profile with small clear and large dense core vesicles. Arrowheads point to presynaptic densities with accumulations of clear synaptic vesicles. The asterisk marks a profile with large granular vesicles. The membrane of most vesicles is broken (arrows). These have a lighter granular content than intact vesicles (white arrowheads). (D) Magnified detail from Figure 2C . Profiles N1 and N2 with small clear and dark dense core vesicles are presynaptic to a third small profile (asterisk). In adjacent sections, this profile contained large granular vesicles. Presynaptic structures consist of a bar-shaped density surrounded by small clear vesicles. The synapses may be monadic or dyadic. Scale bar in (D) : 0.5 μm (applies to A–D ).

    Article Snippet: Data Evaluation and Statistics Ultrathin sections were examined and photographed with a transmission electron microscope (EM 109 and EM10C, Zeiss, Oberkochen, Germany).

    Techniques:

    Virion morphology changes and matrix protein 1 (M1) cytoplasmic disintegration under different pH treatments. ( A ) Representative transmission electron microscopy (TEM) images of purified wild-type A/WSN/33 (WSN), M(NLS-88R) or M(NLS-88E) pretreated with the indicated pH buffers. Images were acquired under a Zeiss EM 912 transmission electron microscope equipped with a Keenview digital camera. Black and tan arrows indicate M1 layer and spikes, respectively. ( B ) The percentages of partially spiked virions per ∼100 viral particles blindly counted by two individuals. The data are expressed as the average±SEM. ( C ) The average M1 thickness in different pH pretreated virions under TEM ( n =10 virions). *** P

    Journal: Emerging Microbes & Infections

    Article Title: Maintaining pH-dependent conformational flexibility of M1 is critical for efficient influenza A virus replication

    doi: 10.1038/emi.2017.96

    Figure Lengend Snippet: Virion morphology changes and matrix protein 1 (M1) cytoplasmic disintegration under different pH treatments. ( A ) Representative transmission electron microscopy (TEM) images of purified wild-type A/WSN/33 (WSN), M(NLS-88R) or M(NLS-88E) pretreated with the indicated pH buffers. Images were acquired under a Zeiss EM 912 transmission electron microscope equipped with a Keenview digital camera. Black and tan arrows indicate M1 layer and spikes, respectively. ( B ) The percentages of partially spiked virions per ∼100 viral particles blindly counted by two individuals. The data are expressed as the average±SEM. ( C ) The average M1 thickness in different pH pretreated virions under TEM ( n =10 virions). *** P

    Article Snippet: Ultrathin sections were stained with uranyl acetate and lead citrate and were examined under a Zeiss EM 912 transmission electron microscope (TEM) (Thornwood, NY, USA) equipped with a Keenview digital camera (Olympus).

    Techniques: Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Purification, Microscopy