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CEM Corporation ultrathin sections
Images of recombinant P. pastoris producing HBsAg during growth on methanol . (A-D) Representative transmission electron micrographs of <t>ultrathin</t> sectioned cells of P. pastoris GS115 grown for 151 h on methanol as described in the Materials and Methods section. Abbreviations as specified in Figure 1.
Ultrathin Sections, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen"

Article Title: Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

Journal: Microbial Cell Factories

doi: 10.1186/1475-2859-10-48

Images of recombinant P. pastoris producing HBsAg during growth on methanol . (A-D) Representative transmission electron micrographs of ultrathin sectioned cells of P. pastoris GS115 grown for 151 h on methanol as described in the Materials and Methods section. Abbreviations as specified in Figure 1.
Figure Legend Snippet: Images of recombinant P. pastoris producing HBsAg during growth on methanol . (A-D) Representative transmission electron micrographs of ultrathin sectioned cells of P. pastoris GS115 grown for 151 h on methanol as described in the Materials and Methods section. Abbreviations as specified in Figure 1.

Techniques Used: Recombinant, Transmission Assay

Images of recombinant P. pastoris growing on glycerol and methanol . Transmission electron microscopy of ultrathin sectioned cells of P. pastoris GS115 growing on (A) glycerol and (B) after 151 h growth on methanol for induction of HBsAg production. (C) Image of P. pastoris X-33 after 96 h growth on methanol for induction of secretory insulin precursor production [ 38 ]. Abbreviations: n, nucleus; v, vacuole; m, mitochondrion; r, ribosome; p, peroxisome; *, 'irregular cloud-shaped area'. The insets show close-ups of ribosomes from marked areas of respective images.
Figure Legend Snippet: Images of recombinant P. pastoris growing on glycerol and methanol . Transmission electron microscopy of ultrathin sectioned cells of P. pastoris GS115 growing on (A) glycerol and (B) after 151 h growth on methanol for induction of HBsAg production. (C) Image of P. pastoris X-33 after 96 h growth on methanol for induction of secretory insulin precursor production [ 38 ]. Abbreviations: n, nucleus; v, vacuole; m, mitochondrion; r, ribosome; p, peroxisome; *, 'irregular cloud-shaped area'. The insets show close-ups of ribosomes from marked areas of respective images.

Techniques Used: Recombinant, Transmission Assay, Electron Microscopy

2) Product Images from "Jab1 regulates Schwann cell proliferation and axonal sorting through p27"

Article Title: Jab1 regulates Schwann cell proliferation and axonal sorting through p27

Journal: The Journal of Experimental Medicine

doi: 10.1084/jem.20130720

Reduced levels of p27 rescue the peripheral nerve phenotype in Jab1-null mice. (A) Semithin and ultrathin sections of sciatic nerve from P30 Jab1 −/− and double Jab1 −/− p27 −/− mice. (B) Morphometric analysis of the endoneurial area occupied by bundles of unsorted axons, number of axons per each bundle, mean bundle size, and number of sorted myelinated axons in Jab1 −/− and double Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (C) Analysis of fiber type distribution per axon diameter, mean g ratio, and g ratio per axon diameter in WT ( n = 3 mice per genotype) and Jab1 −/− and double Jab1 −/− p27 −/− mice ( n = 5 mice per genotype). (D) Neurophysiology showing NCV and cMAP in P30 WT, Jab1 −/− , and double Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (E) Quantification of Schwann cell number in P5 sciatic nerves of WT, Jab1 −/− , and Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (F) Percentage quantification of nuclei double positive for BrdU and Ki67 staining on the total number of Ki67-positive nuclei in sciatic nerve sections of P5 WT, Jab1 −/− , and Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). A representative immunofluorescence image of Jab1 −/− p27 −/− mice is shown. Bars: (A, top) 20 µm; (A, bottom) 2 µm; (F) 30 µm. Paired Student’s t test: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. Error bars indicate SEM.
Figure Legend Snippet: Reduced levels of p27 rescue the peripheral nerve phenotype in Jab1-null mice. (A) Semithin and ultrathin sections of sciatic nerve from P30 Jab1 −/− and double Jab1 −/− p27 −/− mice. (B) Morphometric analysis of the endoneurial area occupied by bundles of unsorted axons, number of axons per each bundle, mean bundle size, and number of sorted myelinated axons in Jab1 −/− and double Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (C) Analysis of fiber type distribution per axon diameter, mean g ratio, and g ratio per axon diameter in WT ( n = 3 mice per genotype) and Jab1 −/− and double Jab1 −/− p27 −/− mice ( n = 5 mice per genotype). (D) Neurophysiology showing NCV and cMAP in P30 WT, Jab1 −/− , and double Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (E) Quantification of Schwann cell number in P5 sciatic nerves of WT, Jab1 −/− , and Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (F) Percentage quantification of nuclei double positive for BrdU and Ki67 staining on the total number of Ki67-positive nuclei in sciatic nerve sections of P5 WT, Jab1 −/− , and Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). A representative immunofluorescence image of Jab1 −/− p27 −/− mice is shown. Bars: (A, top) 20 µm; (A, bottom) 2 µm; (F) 30 µm. Paired Student’s t test: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. Error bars indicate SEM.

Techniques Used: Mouse Assay, Staining, Immunofluorescence

Ablation of Jab1 results in defective axon sorting and myelination. (A and B) Semithin sections of P60 sciatic nerves from WT (A) and Jab1 −/− (B) mice. Arrows identify bundles of unsorted axons in mutant nerves. (C) Electron micrographs of P60 sciatic nerves from Jab1 −/− mice. Bundles of unsorted axons contain axons of different caliber, including those with diameter > 1 µm. Bundles are entirely surrounded by Schwann cells that protrude processes within the axons; the boxed area is magnified below, and arrowheads identify Schwann cell protrusions within unsorted axons. (D) Electron micrographs of P60 sciatic nerves from Jab1 −/− mice showing sorted but not myelinated axons and discontinuous basal lamina (arrows). (E) Electron micrographs of P90 sciatic nerves from Jab1 −/− mice showing bundles of unsorted axons entirely myelinated (polyaxonal myelination). (F) Semithin and ultrathin sections of 6-mo-old sciatic nerve from WT and Jab1 −/− mice. The asterisk identifies Schwann cell degeneration, and the arrow shows axonal degeneration. Bars: (A, B, and F [left]) 20 µm; (C [top], E, and F [right]) 1 µm; (C, bottom) 1.7 µm; (D) 0.5 µm.
Figure Legend Snippet: Ablation of Jab1 results in defective axon sorting and myelination. (A and B) Semithin sections of P60 sciatic nerves from WT (A) and Jab1 −/− (B) mice. Arrows identify bundles of unsorted axons in mutant nerves. (C) Electron micrographs of P60 sciatic nerves from Jab1 −/− mice. Bundles of unsorted axons contain axons of different caliber, including those with diameter > 1 µm. Bundles are entirely surrounded by Schwann cells that protrude processes within the axons; the boxed area is magnified below, and arrowheads identify Schwann cell protrusions within unsorted axons. (D) Electron micrographs of P60 sciatic nerves from Jab1 −/− mice showing sorted but not myelinated axons and discontinuous basal lamina (arrows). (E) Electron micrographs of P90 sciatic nerves from Jab1 −/− mice showing bundles of unsorted axons entirely myelinated (polyaxonal myelination). (F) Semithin and ultrathin sections of 6-mo-old sciatic nerve from WT and Jab1 −/− mice. The asterisk identifies Schwann cell degeneration, and the arrow shows axonal degeneration. Bars: (A, B, and F [left]) 20 µm; (C [top], E, and F [right]) 1 µm; (C, bottom) 1.7 µm; (D) 0.5 µm.

Techniques Used: Mouse Assay, Mutagenesis

Axonal sorting defect is present since early development. (A) Electron micrographs of sciatic nerve from P1 WT and Jab1 −/− mice showing bundles of unsorted axons and myelinated fibers. (B) Semithin and ultrathin sections of sciatic nerve from P5 WT and Jab1 −/− mice showing bundles of unsorted axons only in Jab1 −/− nerves. Bars: (A and B [bottom]) 0.5 µm; (B, top) 10 µm.
Figure Legend Snippet: Axonal sorting defect is present since early development. (A) Electron micrographs of sciatic nerve from P1 WT and Jab1 −/− mice showing bundles of unsorted axons and myelinated fibers. (B) Semithin and ultrathin sections of sciatic nerve from P5 WT and Jab1 −/− mice showing bundles of unsorted axons only in Jab1 −/− nerves. Bars: (A and B [bottom]) 0.5 µm; (B, top) 10 µm.

Techniques Used: Mouse Assay

Related Articles

Staining:

Article Title: Jab1 regulates Schwann cell proliferation and axonal sorting through p27
Article Snippet: .. Ultrathin sections (100–120 nm thick) were stained with uranyl acetate and lead citrate and examined by electron microscopy (CEM 902; Carl Zeiss). ..

Electron Microscopy:

Article Title: Jab1 regulates Schwann cell proliferation and axonal sorting through p27
Article Snippet: .. Ultrathin sections (100–120 nm thick) were stained with uranyl acetate and lead citrate and examined by electron microscopy (CEM 902; Carl Zeiss). ..

Microscopy:

Article Title: Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen
Article Snippet: .. Ultrathin sections were analyzed with an energy-filtered transmission electron microscope (CEM 902, Zeiss, Oberkochen, Germany) in the elastic bright-field mode and images were captured with a 1 × 1 k CCD camera (Proscan, Scheuring, Germany). .. Images of cells prepared for ultrastructural analysis or immunolabeling appear different as sample preparations followed different protocols.

Labeling:

Article Title: p56Lck anchors CD4 to distinct microdomains on microvilli
Article Snippet: .. As judged by coimmunoprecipitation of p56Lck with CD4 (Fig. C ) and CD4 immunogold labeling on ultrathin sections (Fig. E ) from CEM cells, 10 mM 1,10- O -phenanthroline was sufficient to dissociate p56Lck from CD4 and to decrease CD4 association with microvilli to the same extent as what is observed in p56Lck -negative HL60 cells (Fig. E ). .. In conclusion, these morphological analyses demonstrate that p56Lck binding to CD4 targets this receptor to specialized plasma membrane structures, i.e., the microvilli.

Transmission Assay:

Article Title: Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen
Article Snippet: .. Ultrathin sections were analyzed with an energy-filtered transmission electron microscope (CEM 902, Zeiss, Oberkochen, Germany) in the elastic bright-field mode and images were captured with a 1 × 1 k CCD camera (Proscan, Scheuring, Germany). .. Images of cells prepared for ultrastructural analysis or immunolabeling appear different as sample preparations followed different protocols.

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    CEM Corporation ultrathin sections
    Images of recombinant P. pastoris producing HBsAg during growth on methanol . (A-D) Representative transmission electron micrographs of <t>ultrathin</t> sectioned cells of P. pastoris GS115 grown for 151 h on methanol as described in the Materials and Methods section. Abbreviations as specified in Figure 1.
    Ultrathin Sections, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/CEM Corporation
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    85
    CEM Corporation cem 902 transmission electron microscope
    Images of recombinant P. pastoris producing HBsAg during growth on methanol . (A-D) Representative transmission electron micrographs of <t>ultrathin</t> sectioned cells of P. pastoris GS115 grown for 151 h on methanol as described in the Materials and Methods section. Abbreviations as specified in Figure 1.
    Cem 902 Transmission Electron Microscope, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cem 902 transmission electron microscope/product/CEM Corporation
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cem 902 transmission electron microscope - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Images of recombinant P. pastoris producing HBsAg during growth on methanol . (A-D) Representative transmission electron micrographs of ultrathin sectioned cells of P. pastoris GS115 grown for 151 h on methanol as described in the Materials and Methods section. Abbreviations as specified in Figure 1.

    Journal: Microbial Cell Factories

    Article Title: Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

    doi: 10.1186/1475-2859-10-48

    Figure Lengend Snippet: Images of recombinant P. pastoris producing HBsAg during growth on methanol . (A-D) Representative transmission electron micrographs of ultrathin sectioned cells of P. pastoris GS115 grown for 151 h on methanol as described in the Materials and Methods section. Abbreviations as specified in Figure 1.

    Article Snippet: Ultrathin sections were analyzed with an energy-filtered transmission electron microscope (CEM 902, Zeiss, Oberkochen, Germany) in the elastic bright-field mode and images were captured with a 1 × 1 k CCD camera (Proscan, Scheuring, Germany).

    Techniques: Recombinant, Transmission Assay

    Images of recombinant P. pastoris growing on glycerol and methanol . Transmission electron microscopy of ultrathin sectioned cells of P. pastoris GS115 growing on (A) glycerol and (B) after 151 h growth on methanol for induction of HBsAg production. (C) Image of P. pastoris X-33 after 96 h growth on methanol for induction of secretory insulin precursor production [ 38 ]. Abbreviations: n, nucleus; v, vacuole; m, mitochondrion; r, ribosome; p, peroxisome; *, 'irregular cloud-shaped area'. The insets show close-ups of ribosomes from marked areas of respective images.

    Journal: Microbial Cell Factories

    Article Title: Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

    doi: 10.1186/1475-2859-10-48

    Figure Lengend Snippet: Images of recombinant P. pastoris growing on glycerol and methanol . Transmission electron microscopy of ultrathin sectioned cells of P. pastoris GS115 growing on (A) glycerol and (B) after 151 h growth on methanol for induction of HBsAg production. (C) Image of P. pastoris X-33 after 96 h growth on methanol for induction of secretory insulin precursor production [ 38 ]. Abbreviations: n, nucleus; v, vacuole; m, mitochondrion; r, ribosome; p, peroxisome; *, 'irregular cloud-shaped area'. The insets show close-ups of ribosomes from marked areas of respective images.

    Article Snippet: Ultrathin sections were analyzed with an energy-filtered transmission electron microscope (CEM 902, Zeiss, Oberkochen, Germany) in the elastic bright-field mode and images were captured with a 1 × 1 k CCD camera (Proscan, Scheuring, Germany).

    Techniques: Recombinant, Transmission Assay, Electron Microscopy

    Reduced levels of p27 rescue the peripheral nerve phenotype in Jab1-null mice. (A) Semithin and ultrathin sections of sciatic nerve from P30 Jab1 −/− and double Jab1 −/− p27 −/− mice. (B) Morphometric analysis of the endoneurial area occupied by bundles of unsorted axons, number of axons per each bundle, mean bundle size, and number of sorted myelinated axons in Jab1 −/− and double Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (C) Analysis of fiber type distribution per axon diameter, mean g ratio, and g ratio per axon diameter in WT ( n = 3 mice per genotype) and Jab1 −/− and double Jab1 −/− p27 −/− mice ( n = 5 mice per genotype). (D) Neurophysiology showing NCV and cMAP in P30 WT, Jab1 −/− , and double Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (E) Quantification of Schwann cell number in P5 sciatic nerves of WT, Jab1 −/− , and Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (F) Percentage quantification of nuclei double positive for BrdU and Ki67 staining on the total number of Ki67-positive nuclei in sciatic nerve sections of P5 WT, Jab1 −/− , and Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). A representative immunofluorescence image of Jab1 −/− p27 −/− mice is shown. Bars: (A, top) 20 µm; (A, bottom) 2 µm; (F) 30 µm. Paired Student’s t test: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. Error bars indicate SEM.

    Journal: The Journal of Experimental Medicine

    Article Title: Jab1 regulates Schwann cell proliferation and axonal sorting through p27

    doi: 10.1084/jem.20130720

    Figure Lengend Snippet: Reduced levels of p27 rescue the peripheral nerve phenotype in Jab1-null mice. (A) Semithin and ultrathin sections of sciatic nerve from P30 Jab1 −/− and double Jab1 −/− p27 −/− mice. (B) Morphometric analysis of the endoneurial area occupied by bundles of unsorted axons, number of axons per each bundle, mean bundle size, and number of sorted myelinated axons in Jab1 −/− and double Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (C) Analysis of fiber type distribution per axon diameter, mean g ratio, and g ratio per axon diameter in WT ( n = 3 mice per genotype) and Jab1 −/− and double Jab1 −/− p27 −/− mice ( n = 5 mice per genotype). (D) Neurophysiology showing NCV and cMAP in P30 WT, Jab1 −/− , and double Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (E) Quantification of Schwann cell number in P5 sciatic nerves of WT, Jab1 −/− , and Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). (F) Percentage quantification of nuclei double positive for BrdU and Ki67 staining on the total number of Ki67-positive nuclei in sciatic nerve sections of P5 WT, Jab1 −/− , and Jab1 −/− p27 −/− mice ( n = 3 mice per genotype). A representative immunofluorescence image of Jab1 −/− p27 −/− mice is shown. Bars: (A, top) 20 µm; (A, bottom) 2 µm; (F) 30 µm. Paired Student’s t test: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. Error bars indicate SEM.

    Article Snippet: Ultrathin sections (100–120 nm thick) were stained with uranyl acetate and lead citrate and examined by electron microscopy (CEM 902; Carl Zeiss).

    Techniques: Mouse Assay, Staining, Immunofluorescence

    Ablation of Jab1 results in defective axon sorting and myelination. (A and B) Semithin sections of P60 sciatic nerves from WT (A) and Jab1 −/− (B) mice. Arrows identify bundles of unsorted axons in mutant nerves. (C) Electron micrographs of P60 sciatic nerves from Jab1 −/− mice. Bundles of unsorted axons contain axons of different caliber, including those with diameter > 1 µm. Bundles are entirely surrounded by Schwann cells that protrude processes within the axons; the boxed area is magnified below, and arrowheads identify Schwann cell protrusions within unsorted axons. (D) Electron micrographs of P60 sciatic nerves from Jab1 −/− mice showing sorted but not myelinated axons and discontinuous basal lamina (arrows). (E) Electron micrographs of P90 sciatic nerves from Jab1 −/− mice showing bundles of unsorted axons entirely myelinated (polyaxonal myelination). (F) Semithin and ultrathin sections of 6-mo-old sciatic nerve from WT and Jab1 −/− mice. The asterisk identifies Schwann cell degeneration, and the arrow shows axonal degeneration. Bars: (A, B, and F [left]) 20 µm; (C [top], E, and F [right]) 1 µm; (C, bottom) 1.7 µm; (D) 0.5 µm.

    Journal: The Journal of Experimental Medicine

    Article Title: Jab1 regulates Schwann cell proliferation and axonal sorting through p27

    doi: 10.1084/jem.20130720

    Figure Lengend Snippet: Ablation of Jab1 results in defective axon sorting and myelination. (A and B) Semithin sections of P60 sciatic nerves from WT (A) and Jab1 −/− (B) mice. Arrows identify bundles of unsorted axons in mutant nerves. (C) Electron micrographs of P60 sciatic nerves from Jab1 −/− mice. Bundles of unsorted axons contain axons of different caliber, including those with diameter > 1 µm. Bundles are entirely surrounded by Schwann cells that protrude processes within the axons; the boxed area is magnified below, and arrowheads identify Schwann cell protrusions within unsorted axons. (D) Electron micrographs of P60 sciatic nerves from Jab1 −/− mice showing sorted but not myelinated axons and discontinuous basal lamina (arrows). (E) Electron micrographs of P90 sciatic nerves from Jab1 −/− mice showing bundles of unsorted axons entirely myelinated (polyaxonal myelination). (F) Semithin and ultrathin sections of 6-mo-old sciatic nerve from WT and Jab1 −/− mice. The asterisk identifies Schwann cell degeneration, and the arrow shows axonal degeneration. Bars: (A, B, and F [left]) 20 µm; (C [top], E, and F [right]) 1 µm; (C, bottom) 1.7 µm; (D) 0.5 µm.

    Article Snippet: Ultrathin sections (100–120 nm thick) were stained with uranyl acetate and lead citrate and examined by electron microscopy (CEM 902; Carl Zeiss).

    Techniques: Mouse Assay, Mutagenesis

    Axonal sorting defect is present since early development. (A) Electron micrographs of sciatic nerve from P1 WT and Jab1 −/− mice showing bundles of unsorted axons and myelinated fibers. (B) Semithin and ultrathin sections of sciatic nerve from P5 WT and Jab1 −/− mice showing bundles of unsorted axons only in Jab1 −/− nerves. Bars: (A and B [bottom]) 0.5 µm; (B, top) 10 µm.

    Journal: The Journal of Experimental Medicine

    Article Title: Jab1 regulates Schwann cell proliferation and axonal sorting through p27

    doi: 10.1084/jem.20130720

    Figure Lengend Snippet: Axonal sorting defect is present since early development. (A) Electron micrographs of sciatic nerve from P1 WT and Jab1 −/− mice showing bundles of unsorted axons and myelinated fibers. (B) Semithin and ultrathin sections of sciatic nerve from P5 WT and Jab1 −/− mice showing bundles of unsorted axons only in Jab1 −/− nerves. Bars: (A and B [bottom]) 0.5 µm; (B, top) 10 µm.

    Article Snippet: Ultrathin sections (100–120 nm thick) were stained with uranyl acetate and lead citrate and examined by electron microscopy (CEM 902; Carl Zeiss).

    Techniques: Mouse Assay