Structured Review

Agrisera ultrathin sections
side of the outer membrane in both pea and Arabidopsis chloroplasts. A to E, <t>Ultrathin</t> sections of pea (A–C) and Arabidopsis (D and E) chloroplasts were hybridized with antibodies against pea Toc75 POTRA-1 (A), pea Toc34G (B), and Arabidopsis Toc75 POTRA domains (D). C was hybridized with nonimmune rabbit IgG, and E was hybridized with IgG purified from the preimmune serum of the anti-Arabidopsis Toc75 POTRA domains antiserum. The sections were then hybridized with gold-conjugated secondary antibodies and observed under an electron microscope. Gold particles are indicated with red arrowheads. Total numbers of particles observed are 544 (A), 705 (B), and 514 (D). F, Percentages of gold particles observed on each side of the outer membrane or that overlap with the outer membrane (OM). See “Materials and Methods” for criteria to determine the positions of particles. Scale bars = 100 nm.
Ultrathin Sections, supplied by Agrisera, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ultrathin sections/product/Agrisera
Average 93 stars, based on 3 article reviews
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ultrathin sections - by Bioz Stars, 2020-08
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1) Product Images from "Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1 [OPEN]"

Article Title: Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1 [OPEN]

Journal: Plant Physiology

doi: 10.1104/pp.16.00919

side of the outer membrane in both pea and Arabidopsis chloroplasts. A to E, Ultrathin sections of pea (A–C) and Arabidopsis (D and E) chloroplasts were hybridized with antibodies against pea Toc75 POTRA-1 (A), pea Toc34G (B), and Arabidopsis Toc75 POTRA domains (D). C was hybridized with nonimmune rabbit IgG, and E was hybridized with IgG purified from the preimmune serum of the anti-Arabidopsis Toc75 POTRA domains antiserum. The sections were then hybridized with gold-conjugated secondary antibodies and observed under an electron microscope. Gold particles are indicated with red arrowheads. Total numbers of particles observed are 544 (A), 705 (B), and 514 (D). F, Percentages of gold particles observed on each side of the outer membrane or that overlap with the outer membrane (OM). See “Materials and Methods” for criteria to determine the positions of particles. Scale bars = 100 nm.
Figure Legend Snippet: side of the outer membrane in both pea and Arabidopsis chloroplasts. A to E, Ultrathin sections of pea (A–C) and Arabidopsis (D and E) chloroplasts were hybridized with antibodies against pea Toc75 POTRA-1 (A), pea Toc34G (B), and Arabidopsis Toc75 POTRA domains (D). C was hybridized with nonimmune rabbit IgG, and E was hybridized with IgG purified from the preimmune serum of the anti-Arabidopsis Toc75 POTRA domains antiserum. The sections were then hybridized with gold-conjugated secondary antibodies and observed under an electron microscope. Gold particles are indicated with red arrowheads. Total numbers of particles observed are 544 (A), 705 (B), and 514 (D). F, Percentages of gold particles observed on each side of the outer membrane or that overlap with the outer membrane (OM). See “Materials and Methods” for criteria to determine the positions of particles. Scale bars = 100 nm.

Techniques Used: Purification, Microscopy

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Incubation:

Article Title: Degradation of the antiviral component ARGONAUTE1 by the autophagy pathway
Article Snippet: .. Ultrathin sections were cut on a Leica Ultracut S (Leica) and incubated with antibodies against GFP (Agrisera) or the V-PPase (CosmoBio) at a dilution of 1:2,000 or 1:4,000, respectively, followed by incubation with 10-nm gold-coupled secondary antibodies (BBInternational) at a dilution of 1:50 in PBS supplemented with 1% BSA. .. Sections were examined in a JEM1400 transmission electron microscope (JEOL) operating at 80 kV.

Article Title: MdGGT1 Impacts Apple miR156 Precursor Levels via Ontogenetic Changes in Subcellular Glutathione Homeostasis
Article Snippet: .. Briefly, the ultrathin sections from J and A leaves were initially incubated with the primary antibody (anti-glutathione rabbit polyclonal IgG, Agrisera Corp., Vännäs, Sweden) at a dilution of 1:3,000 in the dilution buffer. ..

Immunolabeling:

Article Title: Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1 [OPEN]
Article Snippet: .. Ultrathin sections were immunolabeled with an antibody raised against the first POTRA domain of pea Toc75 (pea Toc75POTRA-1, Agrisera AS08 345), then with colloidal gold-conjugated secondary antibodies, and examined under an electron microscope. ..

Microscopy:

Article Title: Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1 [OPEN]
Article Snippet: .. Ultrathin sections were immunolabeled with an antibody raised against the first POTRA domain of pea Toc75 (pea Toc75POTRA-1, Agrisera AS08 345), then with colloidal gold-conjugated secondary antibodies, and examined under an electron microscope. ..

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    Agrisera ultrathin sections
    side of the outer membrane in both pea and Arabidopsis chloroplasts. A to E, <t>Ultrathin</t> sections of pea (A–C) and Arabidopsis (D and E) chloroplasts were hybridized with antibodies against pea Toc75 POTRA-1 (A), pea Toc34G (B), and Arabidopsis Toc75 POTRA domains (D). C was hybridized with nonimmune rabbit IgG, and E was hybridized with IgG purified from the preimmune serum of the anti-Arabidopsis Toc75 POTRA domains antiserum. The sections were then hybridized with gold-conjugated secondary antibodies and observed under an electron microscope. Gold particles are indicated with red arrowheads. Total numbers of particles observed are 544 (A), 705 (B), and 514 (D). F, Percentages of gold particles observed on each side of the outer membrane or that overlap with the outer membrane (OM). See “Materials and Methods” for criteria to determine the positions of particles. Scale bars = 100 nm.
    Ultrathin Sections, supplied by Agrisera, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/Agrisera
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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    side of the outer membrane in both pea and Arabidopsis chloroplasts. A to E, Ultrathin sections of pea (A–C) and Arabidopsis (D and E) chloroplasts were hybridized with antibodies against pea Toc75 POTRA-1 (A), pea Toc34G (B), and Arabidopsis Toc75 POTRA domains (D). C was hybridized with nonimmune rabbit IgG, and E was hybridized with IgG purified from the preimmune serum of the anti-Arabidopsis Toc75 POTRA domains antiserum. The sections were then hybridized with gold-conjugated secondary antibodies and observed under an electron microscope. Gold particles are indicated with red arrowheads. Total numbers of particles observed are 544 (A), 705 (B), and 514 (D). F, Percentages of gold particles observed on each side of the outer membrane or that overlap with the outer membrane (OM). See “Materials and Methods” for criteria to determine the positions of particles. Scale bars = 100 nm.

    Journal: Plant Physiology

    Article Title: Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1Polypeptide Transport-Associated Domains of the Toc75 Channel Protein Are Located in the Intermembrane Space of Chloroplasts 1 [OPEN]

    doi: 10.1104/pp.16.00919

    Figure Lengend Snippet: side of the outer membrane in both pea and Arabidopsis chloroplasts. A to E, Ultrathin sections of pea (A–C) and Arabidopsis (D and E) chloroplasts were hybridized with antibodies against pea Toc75 POTRA-1 (A), pea Toc34G (B), and Arabidopsis Toc75 POTRA domains (D). C was hybridized with nonimmune rabbit IgG, and E was hybridized with IgG purified from the preimmune serum of the anti-Arabidopsis Toc75 POTRA domains antiserum. The sections were then hybridized with gold-conjugated secondary antibodies and observed under an electron microscope. Gold particles are indicated with red arrowheads. Total numbers of particles observed are 544 (A), 705 (B), and 514 (D). F, Percentages of gold particles observed on each side of the outer membrane or that overlap with the outer membrane (OM). See “Materials and Methods” for criteria to determine the positions of particles. Scale bars = 100 nm.

    Article Snippet: Ultrathin sections were immunolabeled with an antibody raised against the first POTRA domain of pea Toc75 (pea Toc75POTRA-1, Agrisera AS08 345), then with colloidal gold-conjugated secondary antibodies, and examined under an electron microscope.

    Techniques: Purification, Microscopy