Structured Review

JEOL ultrathin sectioning
Selected sections of a serial reconstruction of small zonula adherens-like contacts between Type A cells. The junctions between two Type A cells were photographed in 39 <t>ultrathin</t> serial sections; section number is indicated in the lower right corner . Four junctions ( I, II, III, IV ) appear in this reconstruction. The junctions are not continuous but are disk-like in shape with a diameter of ∼0.5–1 μm. Endocytic vesicles ( arrows ) frequently were associated with these junctions. Magnification, 12,500×.
Ultrathin Sectioning, supplied by JEOL, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ultrathin sectioning/product/JEOL
Average 91 stars, based on 16 article reviews
Price from $9.99 to $1999.99
ultrathin sectioning - by Bioz Stars, 2020-08
91/100 stars

Images

1) Product Images from "Cellular Composition and Three-Dimensional Organization of the Subventricular Germinal Zone in the Adult Mammalian Brain"

Article Title: Cellular Composition and Three-Dimensional Organization of the Subventricular Germinal Zone in the Adult Mammalian Brain

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.17-13-05046.1997

Selected sections of a serial reconstruction of small zonula adherens-like contacts between Type A cells. The junctions between two Type A cells were photographed in 39 ultrathin serial sections; section number is indicated in the lower right corner . Four junctions ( I, II, III, IV ) appear in this reconstruction. The junctions are not continuous but are disk-like in shape with a diameter of ∼0.5–1 μm. Endocytic vesicles ( arrows ) frequently were associated with these junctions. Magnification, 12,500×.
Figure Legend Snippet: Selected sections of a serial reconstruction of small zonula adherens-like contacts between Type A cells. The junctions between two Type A cells were photographed in 39 ultrathin serial sections; section number is indicated in the lower right corner . Four junctions ( I, II, III, IV ) appear in this reconstruction. The junctions are not continuous but are disk-like in shape with a diameter of ∼0.5–1 μm. Endocytic vesicles ( arrows ) frequently were associated with these junctions. Magnification, 12,500×.

Techniques Used:

Relationships among the major cell types in the SVZ. Identification of the individual cell types was made in serial ultrathin sections. A , Frontal section through the SVZ in the lateral wall revealing the topographical relationships of the different cell types to one another. A transversely cut chain of Type A ( a ) cells is separated from ependymal cells ( e ) by the electron-lucent lateral expansions of Type B1 cells ( b 1 ), which are found adjacent to the ependymal layer. In contrast, the processes of Type B2 cells ( b 2 ), which are localized basally adjacent to the striatal parenchyma, isolate the chain of Type A cells from the surrounding neuropil. Type B cells are never found inside chains. Notice the clumped chromatin typical of Type B2 cells and the unclumped chromatin of Type B1 cells. A Type C cell ( c ) with its typical reticulated nucleolus is located close to the chain of Type A cells. Magnification, 6700×. B , A thin lamina ( arrows ) formed by individual or multiple processes of Type B1 cells separate Type A cells ( a ) from ependymal cells ( e ). Magnification, 16,800×. C , Tangential section through the SVZ showing the chain organization of Type A cells at the light microscope in a semithin section. The arrows indicate the presence of two chains of dark cells corresponding to Type A cells. Magnification, 360×. D , Tangential section through the SVZ showing the elongated shape of Type A cells in this plane and their close association with Type C cells. The larger size of Type C cells is clearly visible here, as are the differences in cytoplasmic electron density of Type A, B, and C cells. a , Type A cell; b , Type B cell; c , Type C cell. Magnification, 3500×.
Figure Legend Snippet: Relationships among the major cell types in the SVZ. Identification of the individual cell types was made in serial ultrathin sections. A , Frontal section through the SVZ in the lateral wall revealing the topographical relationships of the different cell types to one another. A transversely cut chain of Type A ( a ) cells is separated from ependymal cells ( e ) by the electron-lucent lateral expansions of Type B1 cells ( b 1 ), which are found adjacent to the ependymal layer. In contrast, the processes of Type B2 cells ( b 2 ), which are localized basally adjacent to the striatal parenchyma, isolate the chain of Type A cells from the surrounding neuropil. Type B cells are never found inside chains. Notice the clumped chromatin typical of Type B2 cells and the unclumped chromatin of Type B1 cells. A Type C cell ( c ) with its typical reticulated nucleolus is located close to the chain of Type A cells. Magnification, 6700×. B , A thin lamina ( arrows ) formed by individual or multiple processes of Type B1 cells separate Type A cells ( a ) from ependymal cells ( e ). Magnification, 16,800×. C , Tangential section through the SVZ showing the chain organization of Type A cells at the light microscope in a semithin section. The arrows indicate the presence of two chains of dark cells corresponding to Type A cells. Magnification, 360×. D , Tangential section through the SVZ showing the elongated shape of Type A cells in this plane and their close association with Type C cells. The larger size of Type C cells is clearly visible here, as are the differences in cytoplasmic electron density of Type A, B, and C cells. a , Type A cell; b , Type B cell; c , Type C cell. Magnification, 3500×.

Techniques Used: Light Microscopy

2) Product Images from "Fabry Disease: Preclinical Studies Demonstrate the Effectiveness of ?-Galactosidase A Replacement in Enzyme-Deficient Mice"

Article Title: Fabry Disease: Preclinical Studies Demonstrate the Effectiveness of ?-Galactosidase A Replacement in Enzyme-Deficient Mice

Journal: American Journal of Human Genetics

doi:

Representative electron micrographs of glutaraldehyde-fixed liver (row 1 ), heart (row 2 ), and kidneys (rows 3 – 5 ) of α-Gal A–deficient mice, before ( a and b series) and 7 d after ( c and d series) enzyme treatment with 3 mg/kg every other day for four doses. Note the presence ( 1a and 1b ) and absence ( 1c and 1d ) of osmophilic dense storage material in Kupffer cell lysosomes in liver sinusoids. In the heart, the storage material was present in the lysosomes of cardiac pericytes and in the pericapillary histiocytes and fibroblasts ( 2a and 2b, arrows ), whereas these deposits were not observed posttreatment ( 2c and 2d ). In the kidneys, numerous lysosomes ( arrows ) containing storage material were present in the pericytes of capillaries ( 3a and 3b ), in the cells lining the proximal convoluted tubules ( 4a and 4b ), and in the apical cytoplasm of the cells lining the distal convoluted tubules ( 5a and 5b ). In contrast, the stored material was absent or markedly reduced in these renal cells posttreatment ( 3c and 3d, 4c and 4d, and 5c and 5d, respectively). Ultrathin sections were stained with uranyl acetate and lead citrate.
Figure Legend Snippet: Representative electron micrographs of glutaraldehyde-fixed liver (row 1 ), heart (row 2 ), and kidneys (rows 3 – 5 ) of α-Gal A–deficient mice, before ( a and b series) and 7 d after ( c and d series) enzyme treatment with 3 mg/kg every other day for four doses. Note the presence ( 1a and 1b ) and absence ( 1c and 1d ) of osmophilic dense storage material in Kupffer cell lysosomes in liver sinusoids. In the heart, the storage material was present in the lysosomes of cardiac pericytes and in the pericapillary histiocytes and fibroblasts ( 2a and 2b, arrows ), whereas these deposits were not observed posttreatment ( 2c and 2d ). In the kidneys, numerous lysosomes ( arrows ) containing storage material were present in the pericytes of capillaries ( 3a and 3b ), in the cells lining the proximal convoluted tubules ( 4a and 4b ), and in the apical cytoplasm of the cells lining the distal convoluted tubules ( 5a and 5b ). In contrast, the stored material was absent or markedly reduced in these renal cells posttreatment ( 3c and 3d, 4c and 4d, and 5c and 5d, respectively). Ultrathin sections were stained with uranyl acetate and lead citrate.

Techniques Used: Mouse Assay, Staining

Related Articles

Electron Microscopy:

Article Title: Fabry Disease: Preclinical Studies Demonstrate the Effectiveness of ?-Galactosidase A Replacement in Enzyme-Deficient Mice
Article Snippet: .. One-micron plastic sections were cut, stained with methylene blue and azure II, and observed by light microscopy, to choose representative areas for ultrathin sectioning and examination by electron microscopy using a JEM 100CX electron-transmission microscope (Jeol). .. compares the physical and kinetic properties of the four recombinant human α-Gal A secreted glycoforms.

In Situ:

Article Title: Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro
Article Snippet: .. To in situ visualize BPNPs within the cells of Synechococcus 7002, we fixed algae cells with 2.5% glutaraldehyde in 0.1 M of PBS (136.89 mM of NaCl, 2.67 mM of KCl, 8.1 mM of Na2 HPO4 , 1.76 mM of KH2 PO4 ) for 2 h at 25 °C and for another 12 h in a 4 °C refrigerator, followed by staining with 1% OsO4 for 30 min, ultrathin sectioning and observation under a JEM-1200 electron microscope (JEOL, Tokyo, Japan) operating at 80 kV. .. For TEM measurements of purified BPNPs, sample solutions were dropped onto carbon-coated copper grids, air-dried, and examined in a JEM-1200 electron microscope at 80 kV.

Microscopy:

Article Title: Huaier Extract Induces Autophagic Cell Death by Inhibiting the mTOR/S6K Pathway in Breast Cancer Cells
Article Snippet: .. Representative areas were chosen for ultrathin sectioning and viewed with a JEM 1010 transmission electron microscope (JEOL, Peabody, MA) at an accelerating voltage of 80 kV. .. Digital images were obtained with an AMT imaging system (Advanced Microscopy Techniques, Danvers, MA).

Article Title: Cellular Composition and Three-Dimensional Organization of the Subventricular Germinal Zone in the Adult Mammalian Brain
Article Snippet: .. Two-micron-thick semithin sections were cut with a glass knife and stained with 1% toluidine blue, re-embedded as described above for ultrathin sectioning, and examined under a Jeol 100CX electron microscope. ..

Article Title: Human proximal tubule epithelial cells cultured on hollow fibers: living membranes that actively transport organic cations
Article Snippet: .. After ultrathin sectioning and contrasting with uranyl acetate and lead citrate, sections were analyzed using a transmission electron microscope JEOL JEM 1010 (Jeol, Akishima Tokyo, Japan). ..

Article Title: Virus Factories of Cauliflower Mosaic Virus Are Virion Reservoirs That Engage Actively in Vector Transmission
Article Snippet: .. After ultrathin sectioning, the grids bearing sections were observed in a Jeol JEM 100CX II electron microscope (Jeol) operated at 60 to 80 kV. .. We used the antibodies and immunofluorescence technique described previously ( ).

Article Title: Fabry Disease: Preclinical Studies Demonstrate the Effectiveness of ?-Galactosidase A Replacement in Enzyme-Deficient Mice
Article Snippet: .. One-micron plastic sections were cut, stained with methylene blue and azure II, and observed by light microscopy, to choose representative areas for ultrathin sectioning and examination by electron microscopy using a JEM 100CX electron-transmission microscope (Jeol). .. compares the physical and kinetic properties of the four recombinant human α-Gal A secreted glycoforms.

Article Title: Inhibition of ADP/ATP Exchange in Receptor-Interacting Protein-Mediated Necrosis †
Article Snippet: .. Samples were then postfixed in cacodylate-buffered 1% osmium tetroxide, dehydrated, embedded in Epon 812 (Nacalai Tesque, Osaka, Japan) for ultrathin sectioning, and then stained with uranyl acetate and lead citrate and examined with a JEOL 1220 electron microscope. .. THP-1 cells were treated with TNF-α (10 ng/ml) for 8 h and then incubated with Mitotracker Red (50 nM) in the presence or absence of VAD-FITC-fmk (Promega, Madison, WI) (10 μM) for 30 min.

Article Title: Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro
Article Snippet: .. To in situ visualize BPNPs within the cells of Synechococcus 7002, we fixed algae cells with 2.5% glutaraldehyde in 0.1 M of PBS (136.89 mM of NaCl, 2.67 mM of KCl, 8.1 mM of Na2 HPO4 , 1.76 mM of KH2 PO4 ) for 2 h at 25 °C and for another 12 h in a 4 °C refrigerator, followed by staining with 1% OsO4 for 30 min, ultrathin sectioning and observation under a JEM-1200 electron microscope (JEOL, Tokyo, Japan) operating at 80 kV. .. For TEM measurements of purified BPNPs, sample solutions were dropped onto carbon-coated copper grids, air-dried, and examined in a JEM-1200 electron microscope at 80 kV.

Light Microscopy:

Article Title: Fabry Disease: Preclinical Studies Demonstrate the Effectiveness of ?-Galactosidase A Replacement in Enzyme-Deficient Mice
Article Snippet: .. One-micron plastic sections were cut, stained with methylene blue and azure II, and observed by light microscopy, to choose representative areas for ultrathin sectioning and examination by electron microscopy using a JEM 100CX electron-transmission microscope (Jeol). .. compares the physical and kinetic properties of the four recombinant human α-Gal A secreted glycoforms.

Transmission Assay:

Article Title: Huaier Extract Induces Autophagic Cell Death by Inhibiting the mTOR/S6K Pathway in Breast Cancer Cells
Article Snippet: .. Representative areas were chosen for ultrathin sectioning and viewed with a JEM 1010 transmission electron microscope (JEOL, Peabody, MA) at an accelerating voltage of 80 kV. .. Digital images were obtained with an AMT imaging system (Advanced Microscopy Techniques, Danvers, MA).

Article Title: Human proximal tubule epithelial cells cultured on hollow fibers: living membranes that actively transport organic cations
Article Snippet: .. After ultrathin sectioning and contrasting with uranyl acetate and lead citrate, sections were analyzed using a transmission electron microscope JEOL JEM 1010 (Jeol, Akishima Tokyo, Japan). ..

Staining:

Article Title: Cellular Composition and Three-Dimensional Organization of the Subventricular Germinal Zone in the Adult Mammalian Brain
Article Snippet: .. Two-micron-thick semithin sections were cut with a glass knife and stained with 1% toluidine blue, re-embedded as described above for ultrathin sectioning, and examined under a Jeol 100CX electron microscope. ..

Article Title: Fabry Disease: Preclinical Studies Demonstrate the Effectiveness of ?-Galactosidase A Replacement in Enzyme-Deficient Mice
Article Snippet: .. One-micron plastic sections were cut, stained with methylene blue and azure II, and observed by light microscopy, to choose representative areas for ultrathin sectioning and examination by electron microscopy using a JEM 100CX electron-transmission microscope (Jeol). .. compares the physical and kinetic properties of the four recombinant human α-Gal A secreted glycoforms.

Article Title: Inhibition of ADP/ATP Exchange in Receptor-Interacting Protein-Mediated Necrosis †
Article Snippet: .. Samples were then postfixed in cacodylate-buffered 1% osmium tetroxide, dehydrated, embedded in Epon 812 (Nacalai Tesque, Osaka, Japan) for ultrathin sectioning, and then stained with uranyl acetate and lead citrate and examined with a JEOL 1220 electron microscope. .. THP-1 cells were treated with TNF-α (10 ng/ml) for 8 h and then incubated with Mitotracker Red (50 nM) in the presence or absence of VAD-FITC-fmk (Promega, Madison, WI) (10 μM) for 30 min.

Article Title: Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro
Article Snippet: .. To in situ visualize BPNPs within the cells of Synechococcus 7002, we fixed algae cells with 2.5% glutaraldehyde in 0.1 M of PBS (136.89 mM of NaCl, 2.67 mM of KCl, 8.1 mM of Na2 HPO4 , 1.76 mM of KH2 PO4 ) for 2 h at 25 °C and for another 12 h in a 4 °C refrigerator, followed by staining with 1% OsO4 for 30 min, ultrathin sectioning and observation under a JEM-1200 electron microscope (JEOL, Tokyo, Japan) operating at 80 kV. .. For TEM measurements of purified BPNPs, sample solutions were dropped onto carbon-coated copper grids, air-dried, and examined in a JEM-1200 electron microscope at 80 kV.

Transmission Electron Microscopy:

Article Title: Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model
Article Snippet: .. TEM BeWo b30 cells grown on insert membranes were fixed and processed according to Bode et al. After ultrathin sectioning, the samples were analyzed by TEM (JEM100CXII; JEOL Ltd.). .. Tight junction staining To quantify the integrity of the confluent cell monolayer, immunostaining of tight junctions was performed on day 6 postseeding (PS).

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    JEOL ultrathin sectioning
    Selected sections of a serial reconstruction of small zonula adherens-like contacts between Type A cells. The junctions between two Type A cells were photographed in 39 <t>ultrathin</t> serial sections; section number is indicated in the lower right corner . Four junctions ( I, II, III, IV ) appear in this reconstruction. The junctions are not continuous but are disk-like in shape with a diameter of ∼0.5–1 μm. Endocytic vesicles ( arrows ) frequently were associated with these junctions. Magnification, 12,500×.
    Ultrathin Sectioning, supplied by JEOL, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sectioning/product/JEOL
    Average 91 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    ultrathin sectioning - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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    Selected sections of a serial reconstruction of small zonula adherens-like contacts between Type A cells. The junctions between two Type A cells were photographed in 39 ultrathin serial sections; section number is indicated in the lower right corner . Four junctions ( I, II, III, IV ) appear in this reconstruction. The junctions are not continuous but are disk-like in shape with a diameter of ∼0.5–1 μm. Endocytic vesicles ( arrows ) frequently were associated with these junctions. Magnification, 12,500×.

    Journal: The Journal of Neuroscience

    Article Title: Cellular Composition and Three-Dimensional Organization of the Subventricular Germinal Zone in the Adult Mammalian Brain

    doi: 10.1523/JNEUROSCI.17-13-05046.1997

    Figure Lengend Snippet: Selected sections of a serial reconstruction of small zonula adherens-like contacts between Type A cells. The junctions between two Type A cells were photographed in 39 ultrathin serial sections; section number is indicated in the lower right corner . Four junctions ( I, II, III, IV ) appear in this reconstruction. The junctions are not continuous but are disk-like in shape with a diameter of ∼0.5–1 μm. Endocytic vesicles ( arrows ) frequently were associated with these junctions. Magnification, 12,500×.

    Article Snippet: Two-micron-thick semithin sections were cut with a glass knife and stained with 1% toluidine blue, re-embedded as described above for ultrathin sectioning, and examined under a Jeol 100CX electron microscope.

    Techniques:

    Relationships among the major cell types in the SVZ. Identification of the individual cell types was made in serial ultrathin sections. A , Frontal section through the SVZ in the lateral wall revealing the topographical relationships of the different cell types to one another. A transversely cut chain of Type A ( a ) cells is separated from ependymal cells ( e ) by the electron-lucent lateral expansions of Type B1 cells ( b 1 ), which are found adjacent to the ependymal layer. In contrast, the processes of Type B2 cells ( b 2 ), which are localized basally adjacent to the striatal parenchyma, isolate the chain of Type A cells from the surrounding neuropil. Type B cells are never found inside chains. Notice the clumped chromatin typical of Type B2 cells and the unclumped chromatin of Type B1 cells. A Type C cell ( c ) with its typical reticulated nucleolus is located close to the chain of Type A cells. Magnification, 6700×. B , A thin lamina ( arrows ) formed by individual or multiple processes of Type B1 cells separate Type A cells ( a ) from ependymal cells ( e ). Magnification, 16,800×. C , Tangential section through the SVZ showing the chain organization of Type A cells at the light microscope in a semithin section. The arrows indicate the presence of two chains of dark cells corresponding to Type A cells. Magnification, 360×. D , Tangential section through the SVZ showing the elongated shape of Type A cells in this plane and their close association with Type C cells. The larger size of Type C cells is clearly visible here, as are the differences in cytoplasmic electron density of Type A, B, and C cells. a , Type A cell; b , Type B cell; c , Type C cell. Magnification, 3500×.

    Journal: The Journal of Neuroscience

    Article Title: Cellular Composition and Three-Dimensional Organization of the Subventricular Germinal Zone in the Adult Mammalian Brain

    doi: 10.1523/JNEUROSCI.17-13-05046.1997

    Figure Lengend Snippet: Relationships among the major cell types in the SVZ. Identification of the individual cell types was made in serial ultrathin sections. A , Frontal section through the SVZ in the lateral wall revealing the topographical relationships of the different cell types to one another. A transversely cut chain of Type A ( a ) cells is separated from ependymal cells ( e ) by the electron-lucent lateral expansions of Type B1 cells ( b 1 ), which are found adjacent to the ependymal layer. In contrast, the processes of Type B2 cells ( b 2 ), which are localized basally adjacent to the striatal parenchyma, isolate the chain of Type A cells from the surrounding neuropil. Type B cells are never found inside chains. Notice the clumped chromatin typical of Type B2 cells and the unclumped chromatin of Type B1 cells. A Type C cell ( c ) with its typical reticulated nucleolus is located close to the chain of Type A cells. Magnification, 6700×. B , A thin lamina ( arrows ) formed by individual or multiple processes of Type B1 cells separate Type A cells ( a ) from ependymal cells ( e ). Magnification, 16,800×. C , Tangential section through the SVZ showing the chain organization of Type A cells at the light microscope in a semithin section. The arrows indicate the presence of two chains of dark cells corresponding to Type A cells. Magnification, 360×. D , Tangential section through the SVZ showing the elongated shape of Type A cells in this plane and their close association with Type C cells. The larger size of Type C cells is clearly visible here, as are the differences in cytoplasmic electron density of Type A, B, and C cells. a , Type A cell; b , Type B cell; c , Type C cell. Magnification, 3500×.

    Article Snippet: Two-micron-thick semithin sections were cut with a glass knife and stained with 1% toluidine blue, re-embedded as described above for ultrathin sectioning, and examined under a Jeol 100CX electron microscope.

    Techniques: Light Microscopy

    Representative electron micrographs of glutaraldehyde-fixed liver (row 1 ), heart (row 2 ), and kidneys (rows 3 – 5 ) of α-Gal A–deficient mice, before ( a and b series) and 7 d after ( c and d series) enzyme treatment with 3 mg/kg every other day for four doses. Note the presence ( 1a and 1b ) and absence ( 1c and 1d ) of osmophilic dense storage material in Kupffer cell lysosomes in liver sinusoids. In the heart, the storage material was present in the lysosomes of cardiac pericytes and in the pericapillary histiocytes and fibroblasts ( 2a and 2b, arrows ), whereas these deposits were not observed posttreatment ( 2c and 2d ). In the kidneys, numerous lysosomes ( arrows ) containing storage material were present in the pericytes of capillaries ( 3a and 3b ), in the cells lining the proximal convoluted tubules ( 4a and 4b ), and in the apical cytoplasm of the cells lining the distal convoluted tubules ( 5a and 5b ). In contrast, the stored material was absent or markedly reduced in these renal cells posttreatment ( 3c and 3d, 4c and 4d, and 5c and 5d, respectively). Ultrathin sections were stained with uranyl acetate and lead citrate.

    Journal: American Journal of Human Genetics

    Article Title: Fabry Disease: Preclinical Studies Demonstrate the Effectiveness of ?-Galactosidase A Replacement in Enzyme-Deficient Mice

    doi:

    Figure Lengend Snippet: Representative electron micrographs of glutaraldehyde-fixed liver (row 1 ), heart (row 2 ), and kidneys (rows 3 – 5 ) of α-Gal A–deficient mice, before ( a and b series) and 7 d after ( c and d series) enzyme treatment with 3 mg/kg every other day for four doses. Note the presence ( 1a and 1b ) and absence ( 1c and 1d ) of osmophilic dense storage material in Kupffer cell lysosomes in liver sinusoids. In the heart, the storage material was present in the lysosomes of cardiac pericytes and in the pericapillary histiocytes and fibroblasts ( 2a and 2b, arrows ), whereas these deposits were not observed posttreatment ( 2c and 2d ). In the kidneys, numerous lysosomes ( arrows ) containing storage material were present in the pericytes of capillaries ( 3a and 3b ), in the cells lining the proximal convoluted tubules ( 4a and 4b ), and in the apical cytoplasm of the cells lining the distal convoluted tubules ( 5a and 5b ). In contrast, the stored material was absent or markedly reduced in these renal cells posttreatment ( 3c and 3d, 4c and 4d, and 5c and 5d, respectively). Ultrathin sections were stained with uranyl acetate and lead citrate.

    Article Snippet: One-micron plastic sections were cut, stained with methylene blue and azure II, and observed by light microscopy, to choose representative areas for ultrathin sectioning and examination by electron microscopy using a JEM 100CX electron-transmission microscope (Jeol).

    Techniques: Mouse Assay, Staining