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Collaborative Drug Discovery Inc ultrathin section
Ultrathin Section, supplied by Collaborative Drug Discovery Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Virtual Reconstruction and Three-Dimensional Printing of Blood Cells as a Tool in Cell Biology Education
Article Snippet: Image of a neutrophil in a single ultrathin section (70 nm thick) acquired with a CCD CAMERA (2k x 2k) (Gatan Orius SC200, model 830, Gatan USA) were used to produce a 3D model by artificially increasing the height (z scale) of each structure previously segmented.

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Article Title: Structural correlates of heterogeneous in vivo activity of midbrain dopaminergic neurons
Article Snippet: .. Electron micrographs were acquired of the labeled structures in each ultrathin section (UltraScan 1000 CCD camera, Gatan). ..

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    Collaborative Drug Discovery Inc ultrathin sections
    ORP5 localize at ER-mitochondria contact sites near cristae junctions (CJ) (a) Electron micrographs of <t>ultrathin</t> cryosections of HeLa cells transfected with HA-ORP5 or Mitofilin-EGFP and immunogold stained with anti-HA or anti-GFP (10 or 15 nm gold), showing ORP5 localization at ER-mitochondria contacts in close proximity to CJ (arrow) and the localization of the MICOS complex (Mitofilin) at CJ (arrows). Scale bar 250 nm. (b) Quantification of the proximity of HA-ORP5 and EGFP-Sec61β gold particles to the CJ. Results are presented as the percentage of ORP5 or Sec61β gold particles (n = 23 cell profiles) at specific ranges of distance (in nm) from CJ. (c) Electron micrographs of ultrathin cryosections of HeLa cells transfected with EGFP-ORP5 or GFP-Sec61β and immunogold labeled with anti-GFP (15 nm gold) and anti-PDI (10nm gold). Note ORP5 localization at ER-mitochondria contacts near CJ (arrow) and Sec61β localization to ER membranes not in contact with the mitochondria membranes (arrowheads). Scale bar 250 nm.
    Ultrathin Sections, supplied by Collaborative Drug Discovery Inc, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/Collaborative Drug Discovery Inc
    Average 92 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    92/100 stars
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    ORP5 localize at ER-mitochondria contact sites near cristae junctions (CJ) (a) Electron micrographs of ultrathin cryosections of HeLa cells transfected with HA-ORP5 or Mitofilin-EGFP and immunogold stained with anti-HA or anti-GFP (10 or 15 nm gold), showing ORP5 localization at ER-mitochondria contacts in close proximity to CJ (arrow) and the localization of the MICOS complex (Mitofilin) at CJ (arrows). Scale bar 250 nm. (b) Quantification of the proximity of HA-ORP5 and EGFP-Sec61β gold particles to the CJ. Results are presented as the percentage of ORP5 or Sec61β gold particles (n = 23 cell profiles) at specific ranges of distance (in nm) from CJ. (c) Electron micrographs of ultrathin cryosections of HeLa cells transfected with EGFP-ORP5 or GFP-Sec61β and immunogold labeled with anti-GFP (15 nm gold) and anti-PDI (10nm gold). Note ORP5 localization at ER-mitochondria contacts near CJ (arrow) and Sec61β localization to ER membranes not in contact with the mitochondria membranes (arrowheads). Scale bar 250 nm.

    Journal: bioRxiv

    Article Title: ORP5 Transfers Phosphatidylserine To Mitochondria And Regulates Mitochondrial Calcium Uptake At Endoplasmic Reticulum - Mitochondria Contact Sites

    doi: 10.1101/695577

    Figure Lengend Snippet: ORP5 localize at ER-mitochondria contact sites near cristae junctions (CJ) (a) Electron micrographs of ultrathin cryosections of HeLa cells transfected with HA-ORP5 or Mitofilin-EGFP and immunogold stained with anti-HA or anti-GFP (10 or 15 nm gold), showing ORP5 localization at ER-mitochondria contacts in close proximity to CJ (arrow) and the localization of the MICOS complex (Mitofilin) at CJ (arrows). Scale bar 250 nm. (b) Quantification of the proximity of HA-ORP5 and EGFP-Sec61β gold particles to the CJ. Results are presented as the percentage of ORP5 or Sec61β gold particles (n = 23 cell profiles) at specific ranges of distance (in nm) from CJ. (c) Electron micrographs of ultrathin cryosections of HeLa cells transfected with EGFP-ORP5 or GFP-Sec61β and immunogold labeled with anti-GFP (15 nm gold) and anti-PDI (10nm gold). Note ORP5 localization at ER-mitochondria contacts near CJ (arrow) and Sec61β localization to ER membranes not in contact with the mitochondria membranes (arrowheads). Scale bar 250 nm.

    Article Snippet: Ultrathin sections were counterstained with 2% uranyl acetate and observed under a FEI Tecnai 12 microscope equipped with a CCD (SiS 1kx1k keenView) camera.

    Techniques: Transfection, Staining, Labeling

    The experimental design incorporated in the TEM analysis and data typical of that collected for each case is illustrated. The relative position of the C1 cell group in the brainstem, as well as the four levels of analysis (L1, L2, L3 L4) selected for TEM sampling, are illustrated schematically in figure 1A. The approximate location of the C1 column is shown by the gray area in the sagittal schematic at the top of A; vertical lines extending through the rostrocaudal extent of the nucleus in that diagram illustrate the extent of coronal vibratome sections collected for analysis. The representation of the C1 cell column shown in the expansion at the bottom of A schematically illustrates the two projection-specific and phenotypically-defined populations of neurons known to populate this region. Filled red circles represent C1 reticulospinal neurons that are concentrated at rostral levels of the nucleus. The open red circles represent C1 neurons that colocalize neuropeptide Y, are concentrated caudally in cell column, and project to supraspinal targets. The filled grey circles represent non-C1 neurons that contribute to both pathways. In figure 1B neurons immunopositive for TH illustrate the position of the C1 column in the ventrolateral medulla and delineate the area cut from the vibratome section for TEM analysis. The lower portion of the schematic illustrates the sectioning strategy incorporated in the analysis to ensure precise localization of labeled profiles within the area of C1. Series of ultrathin sections bracketed by toluidine blue stained thick sections were collected on formvar-coated narrow slot copper grids. Landmarks ( e.g. ). AT = axon terminal, N = neuron cytoplasm, Nu = nucleus; asterisks identify blood vessels. Marker bars = 15 μm for A F, 3 μm for D G, and 0.5 μm for E H.

    Journal: Neuroscience

    Article Title: C1 Catecholamine Neurons form Local Circuit Synaptic Connections Within the Rostroventrolateral Medulla of Rat

    doi: 10.1016/j.neuroscience.2012.09.049

    Figure Lengend Snippet: The experimental design incorporated in the TEM analysis and data typical of that collected for each case is illustrated. The relative position of the C1 cell group in the brainstem, as well as the four levels of analysis (L1, L2, L3 L4) selected for TEM sampling, are illustrated schematically in figure 1A. The approximate location of the C1 column is shown by the gray area in the sagittal schematic at the top of A; vertical lines extending through the rostrocaudal extent of the nucleus in that diagram illustrate the extent of coronal vibratome sections collected for analysis. The representation of the C1 cell column shown in the expansion at the bottom of A schematically illustrates the two projection-specific and phenotypically-defined populations of neurons known to populate this region. Filled red circles represent C1 reticulospinal neurons that are concentrated at rostral levels of the nucleus. The open red circles represent C1 neurons that colocalize neuropeptide Y, are concentrated caudally in cell column, and project to supraspinal targets. The filled grey circles represent non-C1 neurons that contribute to both pathways. In figure 1B neurons immunopositive for TH illustrate the position of the C1 column in the ventrolateral medulla and delineate the area cut from the vibratome section for TEM analysis. The lower portion of the schematic illustrates the sectioning strategy incorporated in the analysis to ensure precise localization of labeled profiles within the area of C1. Series of ultrathin sections bracketed by toluidine blue stained thick sections were collected on formvar-coated narrow slot copper grids. Landmarks ( e.g. ). AT = axon terminal, N = neuron cytoplasm, Nu = nucleus; asterisks identify blood vessels. Marker bars = 15 μm for A F, 3 μm for D G, and 0.5 μm for E H.

    Article Snippet: Ultrathin sections were analyzed unstained using a transmission electron microscope (TEM; Morgagni, FEI, Hillsboro, OR) equipped with a CCD camera (Advanced Microscopy Techniques, Danvers, MA).

    Techniques: Transmission Electron Microscopy, Sampling, Labeling, Staining, Marker

    Ultrastructural characterization of HSV plaques in HaCaT cells. Monolayers of HaCaT cells in 35-mm dishes were infected with 100 PFU of HSV-1[17]/well and processed at 24 h postinfection for TEM as described in Materials and Methods. (a) Low-power binocular image of the vertical face of the embedded resin block. This image gives a three-dimensional view along the horizontal surface of the plastic support. Arrows indicate the plaques of clustered piled-up cells, with the size of the arrow indicating the distance from the block front. (b) The block was then sectioned into semithin survey sections (500 to 1,000 nm) and stained with crystal violet. Images were collected on a Zeiss Axiovert 200M using a ×40 objective lens, and the panels were assembled using Adobe Photoshop. (c) Sequential ultrathin sections (50 to 70 nm) of the same sample were then obtained and collected onto slot grids, stained, and examined by TEM. Because the sections analyzed are immediately adjacent to the section stained with crystal violet, corresponding areas could be matched. The TEM images of the sections in panel c, sections 1 to 4, therefore correspond to the boxes in panel b, sections 1 to 4. Upper panels (lower magnification) and lower panels (higher magnification on inset area) show different features of infection within the plaque as discussed in the text. In the lower panels, the features include extracellular virions (black arrows with white outline), nucleocapsids (short black arrows), and cell tight junctions (white arrows). In the upper part of panels 3 and 4, cells with no distinct features of infection (e.g., no early chromatin marginalization) are indicated by long black arrows. Box 5 in panel b shows flat uninfected cells that correspond to the elongated cells (TEM data not shown).

    Journal: Journal of Virology

    Article Title: Polarized Cell Migration during Cell-to-Cell Transmission of Herpes Simplex Virus in Human Skin Keratinocytes

    doi: 10.1128/JVI.01172-13

    Figure Lengend Snippet: Ultrastructural characterization of HSV plaques in HaCaT cells. Monolayers of HaCaT cells in 35-mm dishes were infected with 100 PFU of HSV-1[17]/well and processed at 24 h postinfection for TEM as described in Materials and Methods. (a) Low-power binocular image of the vertical face of the embedded resin block. This image gives a three-dimensional view along the horizontal surface of the plastic support. Arrows indicate the plaques of clustered piled-up cells, with the size of the arrow indicating the distance from the block front. (b) The block was then sectioned into semithin survey sections (500 to 1,000 nm) and stained with crystal violet. Images were collected on a Zeiss Axiovert 200M using a ×40 objective lens, and the panels were assembled using Adobe Photoshop. (c) Sequential ultrathin sections (50 to 70 nm) of the same sample were then obtained and collected onto slot grids, stained, and examined by TEM. Because the sections analyzed are immediately adjacent to the section stained with crystal violet, corresponding areas could be matched. The TEM images of the sections in panel c, sections 1 to 4, therefore correspond to the boxes in panel b, sections 1 to 4. Upper panels (lower magnification) and lower panels (higher magnification on inset area) show different features of infection within the plaque as discussed in the text. In the lower panels, the features include extracellular virions (black arrows with white outline), nucleocapsids (short black arrows), and cell tight junctions (white arrows). In the upper part of panels 3 and 4, cells with no distinct features of infection (e.g., no early chromatin marginalization) are indicated by long black arrows. Box 5 in panel b shows flat uninfected cells that correspond to the elongated cells (TEM data not shown).

    Article Snippet: The sample was sectioned again when appropriate transverse sections were observed, and ultrathin sections (typically 50 to 70 nm) were cut and collected onto slot grids, stained with Reynold's lead citrate examined in a FEI Tecnai electron microscope with CCD camera image acquisition.

    Techniques: Infection, Transmission Electron Microscopy, Blocking Assay, Staining