ultrapure agarose  (Thermo Fisher)


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  • 99
    Name:
    UltraPure Agarose
    Description:
    UltraPure Agarose is a polysaccharide used for size based separation of nucleic acids in agarose gel electrophoresis applications UltraPure Agarose is ideal for resolving DNA and RNA fragments from 100 bp to 30 kb Features of UltraPure Agarose • Ideal for analysis and recovery of DNA and RNA for routine applications• Strong gel structure allows for better handling and less breakage• Can be used in protein electrophoresis applications such as Ouchterlony antigen antibody interaction assay and radial immunodiffusion RID antigen quantitation assay Improved packagingThe new environmentally friendly packaging uses 75 less plastic than the original bottles This means less energy and raw material used in manufacture and less waste in landfills Additionally the easy pour spout reduces the likelihood of spills and contamination Performance and quality testingThe performance of UltraPure Agarose is evaluated to satisfy set specifications in appearance moisture gel strength gelling temperature melting temperature sulfate content electroendosmosis and DNase RNase activity
    Catalog Number:
    16500100
    Price:
    None
    Applications:
    Agarose Gel Electrophoresis|DNA & RNA Purification & Analysis|Pour Your Own Agarose Gels|Nucleic Acid Gel Electrophoresis & Blotting
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher ultrapure agarose
    UltraPure Agarose is a polysaccharide used for size based separation of nucleic acids in agarose gel electrophoresis applications UltraPure Agarose is ideal for resolving DNA and RNA fragments from 100 bp to 30 kb Features of UltraPure Agarose • Ideal for analysis and recovery of DNA and RNA for routine applications• Strong gel structure allows for better handling and less breakage• Can be used in protein electrophoresis applications such as Ouchterlony antigen antibody interaction assay and radial immunodiffusion RID antigen quantitation assay Improved packagingThe new environmentally friendly packaging uses 75 less plastic than the original bottles This means less energy and raw material used in manufacture and less waste in landfills Additionally the easy pour spout reduces the likelihood of spills and contamination Performance and quality testingThe performance of UltraPure Agarose is evaluated to satisfy set specifications in appearance moisture gel strength gelling temperature melting temperature sulfate content electroendosmosis and DNase RNase activity
    https://www.bioz.com/result/ultrapure agarose/product/Thermo Fisher
    Average 99 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    ultrapure agarose - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Invasion Assay:

    Article Title: Differential Effect of Growth Factors on Invasion and Proliferation of Endocrine Resistant Breast Cancer Cells
    Article Snippet: .. Cell Invasion Assay Ultra-pure agarose (Invitrogen) was melted in PBS, then (once cooled below 40°C) supplemented with DMEM with or without 5% FBS or other components (insulin-transferrin-selenium (ITS) (Invitrogen), ITS plus bovine serum albumin (BSA) (Sigma) or IGF-1 to give a final 0.5% solution and allowed to solidify in individual wells of 6 well dishes at room temperature. ..

    Article Title: Blockade of voltage-gated sodium channels inhibits invasion of endocrine-resistant breast cancer cells
    Article Snippet: .. Agarose invasion assay Ultra-pure agarose (Invitrogen) was melted in PBS, supplemented with DMEM containing 5% FBS, and allowed to solidify in individual wells of 6-well dishes at room temperature. ..

    other:

    Article Title: Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel
    Article Snippet: Cartilage Progenitor Cell Spheroid Culture For spheroid culture, silicone molds were used in order to produce the micromolded nonadhesive agarose hydrogel (agarose 2%—Ultrapure Agarose, Invitrogen, Carlsbad, CA, USA—in NaCl 0.9% solution) with 300 μ m or 800 μ m diameter in each circular recesses (MicroTissues 3D Petri Dish, Sigma) according to manufacturer's protocol ( ).

    Agarose Gel Electrophoresis:

    Article Title: Transplacental Transmission of Cutaneous Leishmania mexicana Strain in BALB/c Mice
    Article Snippet: .. A 2.5% ultrapure agarose gel (Invitrogen, Sao Paulo, Brazil) was prepared and diluted in Tris-Acetate-EDTA (TAE) buffer 1× (containing 40 mM Tris·acetate and 1 mM EDTA, pH 8.3), which was boiled until complete dissolution was achieved. .. The samples were mixed with 1 μL bromophenol blue loading dye (10× BlueJuice Gel Loading Buffer; Invitrogen, Sao Paulo, Brazil), and a molecular weight marker (50-bp DNA ladder; Invitrogen, Sao Paulo, Brazil) was loaded in the first well; 5-μL aliquots were taken from each PCR sample and added to the wells, and the gel was run at 60 V for 2 hours.

    Spot Test:

    Article Title: Discovery and Computer Aided Potency Optimization of a Novel Class of Small Molecule CXCR4 Antagonists
    Article Snippet: .. Agarose spot assay [ ] A 0.5% solution was prepared by adding agarose (UltrapureTM low-melting agarose; Invitrogen, Paisley, UK) to sterile PBS and heating the mixture until all agarose particles were dissolved. ..

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    Thermo Fisher ultrapure tris hydrochloride
    Ultrapure Tris Hydrochloride, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrapure tris hydrochloride/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultrapure tris hydrochloride - by Bioz Stars, 2020-08
    92/100 stars
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    90
    Thermo Fisher ultrapure ethidium bromide
    Effect of Rlip-LNA on apoptosis and DNA fragmentation in MDA-MB-231 and MCF7 cells as measured by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and DNA laddering assays. ( A ) Apoptosis by DNA laddering. After 48 h of treatment with CAS or Rlip-LNA, DNA was extracted and subjected to agarose-gel electrophoresis in 2% agarose with a 1 kB DNA ladder. <t>Ethidium-bromide-stained</t> gels were visualized and photographed under 260 nm UV light. ( B , C ) Effect of Rlip depletion on DNA fragmentation in MDA-MB-231 and MCF7 cells as measured by TUNEL. Cells were transfected with Rlip-LNA or control antisense (CAS) for 24 hours, as described in the Materials and Methods. After Rlip depletion, the apoptotic intensity was determined by flow cytometric TUNEL assay. ( B ) Logarithmic dot plots show the percentage of TUNEL-positive cells in different groups (US = unstained) as measured by flow cytometry. Viable cells were identified by gating on forward and side scatters. ( C ) The overlapped peaks (logarithmic histogram) demonstrate the effects as a whole and are expressed as the fluorescence intensity of the number of counts of the TUNEL-positive cells obtained from the statistical analysis of the fluorescence height and mean value of the x-axis displayed by the software. The fluorescence level for discrimination between apoptotic and nonapoptotic cells was set using the control without TdT (terminal deoxynucleotidyl transferase). Cells above this fluorescence value in the TdT-positive sample were considered apoptotic. Analysis was performed using the BD CSampler software (BD Biosciences). At least 10,000 cells were analyzed per staining. Data were obtained from three independent experiments.
    Ultrapure Ethidium Bromide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrapure ethidium bromide/product/Thermo Fisher
    Average 90 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    ultrapure ethidium bromide - by Bioz Stars, 2020-08
    90/100 stars
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    97
    Thermo Fisher ultrapure edta
    Effect of Rlip-LNA on apoptosis and DNA fragmentation in MDA-MB-231 and MCF7 cells as measured by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and DNA laddering assays. ( A ) Apoptosis by DNA laddering. After 48 h of treatment with CAS or Rlip-LNA, DNA was extracted and subjected to agarose-gel electrophoresis in 2% agarose with a 1 kB DNA ladder. <t>Ethidium-bromide-stained</t> gels were visualized and photographed under 260 nm UV light. ( B , C ) Effect of Rlip depletion on DNA fragmentation in MDA-MB-231 and MCF7 cells as measured by TUNEL. Cells were transfected with Rlip-LNA or control antisense (CAS) for 24 hours, as described in the Materials and Methods. After Rlip depletion, the apoptotic intensity was determined by flow cytometric TUNEL assay. ( B ) Logarithmic dot plots show the percentage of TUNEL-positive cells in different groups (US = unstained) as measured by flow cytometry. Viable cells were identified by gating on forward and side scatters. ( C ) The overlapped peaks (logarithmic histogram) demonstrate the effects as a whole and are expressed as the fluorescence intensity of the number of counts of the TUNEL-positive cells obtained from the statistical analysis of the fluorescence height and mean value of the x-axis displayed by the software. The fluorescence level for discrimination between apoptotic and nonapoptotic cells was set using the control without TdT (terminal deoxynucleotidyl transferase). Cells above this fluorescence value in the TdT-positive sample were considered apoptotic. Analysis was performed using the BD CSampler software (BD Biosciences). At least 10,000 cells were analyzed per staining. Data were obtained from three independent experiments.
    Ultrapure Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrapure edta/product/Thermo Fisher
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ultrapure edta - by Bioz Stars, 2020-08
    97/100 stars
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    Effect of Rlip-LNA on apoptosis and DNA fragmentation in MDA-MB-231 and MCF7 cells as measured by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and DNA laddering assays. ( A ) Apoptosis by DNA laddering. After 48 h of treatment with CAS or Rlip-LNA, DNA was extracted and subjected to agarose-gel electrophoresis in 2% agarose with a 1 kB DNA ladder. Ethidium-bromide-stained gels were visualized and photographed under 260 nm UV light. ( B , C ) Effect of Rlip depletion on DNA fragmentation in MDA-MB-231 and MCF7 cells as measured by TUNEL. Cells were transfected with Rlip-LNA or control antisense (CAS) for 24 hours, as described in the Materials and Methods. After Rlip depletion, the apoptotic intensity was determined by flow cytometric TUNEL assay. ( B ) Logarithmic dot plots show the percentage of TUNEL-positive cells in different groups (US = unstained) as measured by flow cytometry. Viable cells were identified by gating on forward and side scatters. ( C ) The overlapped peaks (logarithmic histogram) demonstrate the effects as a whole and are expressed as the fluorescence intensity of the number of counts of the TUNEL-positive cells obtained from the statistical analysis of the fluorescence height and mean value of the x-axis displayed by the software. The fluorescence level for discrimination between apoptotic and nonapoptotic cells was set using the control without TdT (terminal deoxynucleotidyl transferase). Cells above this fluorescence value in the TdT-positive sample were considered apoptotic. Analysis was performed using the BD CSampler software (BD Biosciences). At least 10,000 cells were analyzed per staining. Data were obtained from three independent experiments.

    Journal: Cancers

    Article Title: Rlip Depletion Suppresses Growth of Breast Cancer

    doi: 10.3390/cancers12061446

    Figure Lengend Snippet: Effect of Rlip-LNA on apoptosis and DNA fragmentation in MDA-MB-231 and MCF7 cells as measured by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and DNA laddering assays. ( A ) Apoptosis by DNA laddering. After 48 h of treatment with CAS or Rlip-LNA, DNA was extracted and subjected to agarose-gel electrophoresis in 2% agarose with a 1 kB DNA ladder. Ethidium-bromide-stained gels were visualized and photographed under 260 nm UV light. ( B , C ) Effect of Rlip depletion on DNA fragmentation in MDA-MB-231 and MCF7 cells as measured by TUNEL. Cells were transfected with Rlip-LNA or control antisense (CAS) for 24 hours, as described in the Materials and Methods. After Rlip depletion, the apoptotic intensity was determined by flow cytometric TUNEL assay. ( B ) Logarithmic dot plots show the percentage of TUNEL-positive cells in different groups (US = unstained) as measured by flow cytometry. Viable cells were identified by gating on forward and side scatters. ( C ) The overlapped peaks (logarithmic histogram) demonstrate the effects as a whole and are expressed as the fluorescence intensity of the number of counts of the TUNEL-positive cells obtained from the statistical analysis of the fluorescence height and mean value of the x-axis displayed by the software. The fluorescence level for discrimination between apoptotic and nonapoptotic cells was set using the control without TdT (terminal deoxynucleotidyl transferase). Cells above this fluorescence value in the TdT-positive sample were considered apoptotic. Analysis was performed using the BD CSampler software (BD Biosciences). At least 10,000 cells were analyzed per staining. Data were obtained from three independent experiments.

    Article Snippet: Ethidium-bromide-stained gels were visualized and photographed under 260 nm UV light [ ].

    Techniques: Multiple Displacement Amplification, TUNEL Assay, DNA Laddering, Agarose Gel Electrophoresis, Staining, Transfection, Flow Cytometry, Fluorescence, Software

    Schematic diagrams of the ( a ) FIV CRISPR Cas9 and ( b ) control vectors. Fluorescent microscopy images of MCH5-4 cells infected with the control vector for 2, 6, or 17 days ( c , d , or e , respectively). Images d and e were obtained during selection (puromycin). ( f ) Ethidium bromide-stained agarose gel electrophoresis of PCR amplicons (FIV CRISPR Cas9 nucleotides 2166-2691). The black arrowhead indicates 500 base pairs (appropriate sized amplicon). Lane 1—molecular weight markers, lane 2—plasmid DNA (positive control), lane 3—FIV CRISPR Cas9 infected/selected feline PBMC, lane 4—feline MCH5-4 cells, lane 5—water template (negative control).

    Journal: Viruses

    Article Title: An RNA-Directed Gene Editing Strategy for Attenuating the Infectious Potential of Feline Immunodeficiency Virus-Infected Cells: A Proof of Concept

    doi: 10.3390/v12050511

    Figure Lengend Snippet: Schematic diagrams of the ( a ) FIV CRISPR Cas9 and ( b ) control vectors. Fluorescent microscopy images of MCH5-4 cells infected with the control vector for 2, 6, or 17 days ( c , d , or e , respectively). Images d and e were obtained during selection (puromycin). ( f ) Ethidium bromide-stained agarose gel electrophoresis of PCR amplicons (FIV CRISPR Cas9 nucleotides 2166-2691). The black arrowhead indicates 500 base pairs (appropriate sized amplicon). Lane 1—molecular weight markers, lane 2—plasmid DNA (positive control), lane 3—FIV CRISPR Cas9 infected/selected feline PBMC, lane 4—feline MCH5-4 cells, lane 5—water template (negative control).

    Article Snippet: The resulting PCR products were electrophoresed onto ethidium bromide-stained agarose gel.

    Techniques: CRISPR, Microscopy, Infection, Plasmid Preparation, Selection, Staining, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Molecular Weight, Positive Control, Negative Control