ultraperformance liquid chromatography tandem mass spectrometry  (Waters Corporation)

 
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    Waters Corporation ultraperformance liquid chromatography tandem mass spectrometry
    LC-MS chromatograms (collected with <t>ultraperformance</t> liquid chromatography coupled to a Q-Exactive Orbitrap mass spectrometer) of a series of H. canadensis extract fractions. (A) Positive-ion electrospray mass spectrometry chromatogram of the crude extract after liquid–liquid partitioning. (B) The most active fraction (GS-4) of the crude extract shown in A after separation with flash chromatography over silica gel using a hexane–chloroform–methanol gradient (stage 1 separation). GS-4 was further fractionated using a second stage of flash chromatography over silica gel with a hexane–ethyl acetate–methanol gradient with GS-4-4 being the most active (C). GS-4-4-2 generated with stage 3 fractionation (using reversed-phase preparative HPLC with a water–acetonitrile gradient) (D). (E–H) The same H. canadensis extract and fractions analyzed using LC-MS in the negative-ion mode. Arrows represent the region of the chromatogram in which a given ion corresponding to a known constituent of H. canadensis (indicated by compound numbers) could be detected. Compounds 1 – 7 , 10 , 13 , 16 , 17 , 20 – 24 , and 28 while compounds 8 , 9 , 11 , 12 , 14 , 15 , 18 , 19 , and 25 – 27 29 is a new flavonoid that was isolated and identified based on NMR and MS data as part of this report. Red dotted arrows represent alkaloids and other compounds, while green dashed arrows represent flavonoids. In cases where a specific peak is not apparent in the chromatogram, the ion was identified based on mass spectrometric data.
    Ultraperformance Liquid Chromatography Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultraperformance liquid chromatography tandem mass spectrometry/product/Waters Corporation
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultraperformance liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    88/100 stars

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    1) Product Images from "Biochemometrics to Identify Synergists and Additives from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal)"

    Article Title: Biochemometrics to Identify Synergists and Additives from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal)

    Journal: Journal of natural products

    doi: 10.1021/acs.jnatprod.7b00654

    LC-MS chromatograms (collected with ultraperformance liquid chromatography coupled to a Q-Exactive Orbitrap mass spectrometer) of a series of H. canadensis extract fractions. (A) Positive-ion electrospray mass spectrometry chromatogram of the crude extract after liquid–liquid partitioning. (B) The most active fraction (GS-4) of the crude extract shown in A after separation with flash chromatography over silica gel using a hexane–chloroform–methanol gradient (stage 1 separation). GS-4 was further fractionated using a second stage of flash chromatography over silica gel with a hexane–ethyl acetate–methanol gradient with GS-4-4 being the most active (C). GS-4-4-2 generated with stage 3 fractionation (using reversed-phase preparative HPLC with a water–acetonitrile gradient) (D). (E–H) The same H. canadensis extract and fractions analyzed using LC-MS in the negative-ion mode. Arrows represent the region of the chromatogram in which a given ion corresponding to a known constituent of H. canadensis (indicated by compound numbers) could be detected. Compounds 1 – 7 , 10 , 13 , 16 , 17 , 20 – 24 , and 28 while compounds 8 , 9 , 11 , 12 , 14 , 15 , 18 , 19 , and 25 – 27 29 is a new flavonoid that was isolated and identified based on NMR and MS data as part of this report. Red dotted arrows represent alkaloids and other compounds, while green dashed arrows represent flavonoids. In cases where a specific peak is not apparent in the chromatogram, the ion was identified based on mass spectrometric data.
    Figure Legend Snippet: LC-MS chromatograms (collected with ultraperformance liquid chromatography coupled to a Q-Exactive Orbitrap mass spectrometer) of a series of H. canadensis extract fractions. (A) Positive-ion electrospray mass spectrometry chromatogram of the crude extract after liquid–liquid partitioning. (B) The most active fraction (GS-4) of the crude extract shown in A after separation with flash chromatography over silica gel using a hexane–chloroform–methanol gradient (stage 1 separation). GS-4 was further fractionated using a second stage of flash chromatography over silica gel with a hexane–ethyl acetate–methanol gradient with GS-4-4 being the most active (C). GS-4-4-2 generated with stage 3 fractionation (using reversed-phase preparative HPLC with a water–acetonitrile gradient) (D). (E–H) The same H. canadensis extract and fractions analyzed using LC-MS in the negative-ion mode. Arrows represent the region of the chromatogram in which a given ion corresponding to a known constituent of H. canadensis (indicated by compound numbers) could be detected. Compounds 1 – 7 , 10 , 13 , 16 , 17 , 20 – 24 , and 28 while compounds 8 , 9 , 11 , 12 , 14 , 15 , 18 , 19 , and 25 – 27 29 is a new flavonoid that was isolated and identified based on NMR and MS data as part of this report. Red dotted arrows represent alkaloids and other compounds, while green dashed arrows represent flavonoids. In cases where a specific peak is not apparent in the chromatogram, the ion was identified based on mass spectrometric data.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry, Chromatography, Generated, Fractionation, High Performance Liquid Chromatography, Isolation, Nuclear Magnetic Resonance

    Related Articles

    Mass Spectrometry:

    Article Title: Biochemometrics to Identify Synergists and Additives from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal)
    Article Snippet: .. Extracts were suspended in 1:1 MeOH–dioxane (LC/MS grade, Fisher Scientific, Hampton, NH, USA) at either 1 or 0.1 mg/mL and subjected to ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis via a Waters Acquity UPLC with an Acquity UPLC column (BEH C18 , 1.7 μ m, 2.1 × 50 mm, Waters Corporation, Milford, MA, USA) and a Thermo Q-Exactive Plus orbitrap mass spectrometer with heated electrospray ionization (Thermo Fisher Scientific, Milford, MA, USA). .. Analysis was performed at a flow rate of 0.3 mL/min using the following binary solvent system with A consisting of water with 0.1% formic acid additive and solvent B consisting of acetonitrile with 0.1% formic acid additive (LC/MS grade, Fisher Scientific).

    Article Title: Relationship between seafood consumption and bisphenol A exposure: the Second Korean National Environmental Health Survey (KoNEHS 2012–2014)
    Article Snippet: .. Urinary BPA concentration The concentration of urinary BPA was measured using ultraperformance liquid chromatography–tandem mass spectrometry with Xevo TQ-S (Waters Corporation, Milford, MA, the USA) using a spot urine sample [ ]. ..

    Article Title: Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1 2Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1 2 3Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk As
    Article Snippet: .. To assess vitamin D status , serum 25(OH)D was measured by using Diels-Alder derivatization and ultraperformance liquid chromatography–tandem mass spectrometry (Waters)—a gold standard for evaluating 25(OH)D concentration ( ). ..

    Article Title: Initial Clinical Experience with AZD5718, a Novel Once Daily Oral 5‐Lipoxygenase Activating Protein Inhibitor
    Article Snippet: .. The extracts were injected and analyzed by ultraperformance liquid‐chromatography tandem mass spectrometry (LC‐MS/MS) using an Acquity, BEH C18, 50 × 2.1 mm, particle size 1.7 μm column (Waters, Milford, MA) maintained at 40°C using a gradient of 20–98% acetonitrile in 10 mM ammonium formate (aq, 0.2% formic acid) as the mobile phase. ..

    Article Title: Metabolic Effects of Glucose-Fructose Co-Ingestion Compared to Glucose Alone during Exercise in Type 1 Diabetes
    Article Snippet: .. Plasma catecholamines were quantified using ultraperformance liquid chromatography-tandem mass spectrometry (Waters Acquity UPLC/TQD, Manchester, UK) [ ]. ..

    Article Title: Microbiota-Derived Phenylacetylglutamine Associates with Overall Mortality and Cardiovascular Disease in Patients with CKD
    Article Snippet: .. We also had ancillary data available on free serum levels of p -cresyl sulfate determined as p -cresol with a dedicated gas chromatography-mass spectrometry method, allowing comparison with a protein-bound solute., Additionally, serum levels of PAG were quantified by ultraperformance liquid chromatography-tandem mass spectrometry (Acquity–Xevo TQS; Waters, Milford, MA). .. For sample preparation, 50 μ l serum or urine, 50 μ l solution of milli-Q (MQ) water:MeOH:0.01 N sodium hydroxide (75:20:5 vol/vol/vol), 20 μ l internal standard mixture (PAG-d5), and 150 μ l acetonitrile were thoroughly mixed in 96-well Ostro Plates (Waters).

    Article Title: Genetic Differentiation Associated with Fumonisin and Gibberellin Production in Japanese Fusarium fujikuroi
    Article Snippet: .. The extract filtered through paper was purified by a strong anion-exchange column (Sep-Pak Accell Plus QMA short cartridge [360 mg]; Waters, Milford, MA, USA), and the concentrations of the fumonisins (FB1 , FB2 , and FB3 ) were quantified by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis using an Acquity UPLC system coupled to a Xevo quadrupole time of flight (QTof) mass spectrometer (Waters). .. The fumonisins FB1 (Enzo Life Sciences, Lausen, Switzerland), FB2 (Enzo Life Sciences), and FB3 (Iris Biotech, Marktredwitz, Germany) were used as a standard.

    Chromatography:

    Article Title: Relationship between seafood consumption and bisphenol A exposure: the Second Korean National Environmental Health Survey (KoNEHS 2012–2014)
    Article Snippet: .. Urinary BPA concentration The concentration of urinary BPA was measured using ultraperformance liquid chromatography–tandem mass spectrometry with Xevo TQ-S (Waters Corporation, Milford, MA, the USA) using a spot urine sample [ ]. ..

    Article Title: Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1 2Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1 2 3Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk As
    Article Snippet: .. To assess vitamin D status , serum 25(OH)D was measured by using Diels-Alder derivatization and ultraperformance liquid chromatography–tandem mass spectrometry (Waters)—a gold standard for evaluating 25(OH)D concentration ( ). ..

    Article Title: Metabolic Effects of Glucose-Fructose Co-Ingestion Compared to Glucose Alone during Exercise in Type 1 Diabetes
    Article Snippet: .. Plasma catecholamines were quantified using ultraperformance liquid chromatography-tandem mass spectrometry (Waters Acquity UPLC/TQD, Manchester, UK) [ ]. ..

    Article Title: Microbiota-Derived Phenylacetylglutamine Associates with Overall Mortality and Cardiovascular Disease in Patients with CKD
    Article Snippet: .. We also had ancillary data available on free serum levels of p -cresyl sulfate determined as p -cresol with a dedicated gas chromatography-mass spectrometry method, allowing comparison with a protein-bound solute., Additionally, serum levels of PAG were quantified by ultraperformance liquid chromatography-tandem mass spectrometry (Acquity–Xevo TQS; Waters, Milford, MA). .. For sample preparation, 50 μ l serum or urine, 50 μ l solution of milli-Q (MQ) water:MeOH:0.01 N sodium hydroxide (75:20:5 vol/vol/vol), 20 μ l internal standard mixture (PAG-d5), and 150 μ l acetonitrile were thoroughly mixed in 96-well Ostro Plates (Waters).

    Article Title: Genetic Differentiation Associated with Fumonisin and Gibberellin Production in Japanese Fusarium fujikuroi
    Article Snippet: .. The extract filtered through paper was purified by a strong anion-exchange column (Sep-Pak Accell Plus QMA short cartridge [360 mg]; Waters, Milford, MA, USA), and the concentrations of the fumonisins (FB1 , FB2 , and FB3 ) were quantified by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis using an Acquity UPLC system coupled to a Xevo quadrupole time of flight (QTof) mass spectrometer (Waters). .. The fumonisins FB1 (Enzo Life Sciences, Lausen, Switzerland), FB2 (Enzo Life Sciences), and FB3 (Iris Biotech, Marktredwitz, Germany) were used as a standard.

    Gas Chromatography-Mass Spectrometry:

    Article Title: Microbiota-Derived Phenylacetylglutamine Associates with Overall Mortality and Cardiovascular Disease in Patients with CKD
    Article Snippet: .. We also had ancillary data available on free serum levels of p -cresyl sulfate determined as p -cresol with a dedicated gas chromatography-mass spectrometry method, allowing comparison with a protein-bound solute., Additionally, serum levels of PAG were quantified by ultraperformance liquid chromatography-tandem mass spectrometry (Acquity–Xevo TQS; Waters, Milford, MA). .. For sample preparation, 50 μ l serum or urine, 50 μ l solution of milli-Q (MQ) water:MeOH:0.01 N sodium hydroxide (75:20:5 vol/vol/vol), 20 μ l internal standard mixture (PAG-d5), and 150 μ l acetonitrile were thoroughly mixed in 96-well Ostro Plates (Waters).

    Purification:

    Article Title: Genetic Differentiation Associated with Fumonisin and Gibberellin Production in Japanese Fusarium fujikuroi
    Article Snippet: .. The extract filtered through paper was purified by a strong anion-exchange column (Sep-Pak Accell Plus QMA short cartridge [360 mg]; Waters, Milford, MA, USA), and the concentrations of the fumonisins (FB1 , FB2 , and FB3 ) were quantified by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis using an Acquity UPLC system coupled to a Xevo quadrupole time of flight (QTof) mass spectrometer (Waters). .. The fumonisins FB1 (Enzo Life Sciences, Lausen, Switzerland), FB2 (Enzo Life Sciences), and FB3 (Iris Biotech, Marktredwitz, Germany) were used as a standard.

    Concentration Assay:

    Article Title: Relationship between seafood consumption and bisphenol A exposure: the Second Korean National Environmental Health Survey (KoNEHS 2012–2014)
    Article Snippet: .. Urinary BPA concentration The concentration of urinary BPA was measured using ultraperformance liquid chromatography–tandem mass spectrometry with Xevo TQ-S (Waters Corporation, Milford, MA, the USA) using a spot urine sample [ ]. ..

    Article Title: Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1 2Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk Asian subjects 1 2 3Association of vitamin D deficiency with incidence of type 2 diabetes in high-risk As
    Article Snippet: .. To assess vitamin D status , serum 25(OH)D was measured by using Diels-Alder derivatization and ultraperformance liquid chromatography–tandem mass spectrometry (Waters)—a gold standard for evaluating 25(OH)D concentration ( ). ..

    other:

    Article Title: iNOS Activity Is Required for the Therapeutic Effect of Mesenchymal Stem Cells in Experimental Systemic Sclerosis
    Article Snippet: Concentrations of GSH and its oxidized form GSSG were determined using ultraperformance liquid-chromatography–tandem-mass spectrometry (UPLC, Waters Acquity, Milford, USA).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Biochemometrics to Identify Synergists and Additives from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal)
    Article Snippet: .. Extracts were suspended in 1:1 MeOH–dioxane (LC/MS grade, Fisher Scientific, Hampton, NH, USA) at either 1 or 0.1 mg/mL and subjected to ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis via a Waters Acquity UPLC with an Acquity UPLC column (BEH C18 , 1.7 μ m, 2.1 × 50 mm, Waters Corporation, Milford, MA, USA) and a Thermo Q-Exactive Plus orbitrap mass spectrometer with heated electrospray ionization (Thermo Fisher Scientific, Milford, MA, USA). .. Analysis was performed at a flow rate of 0.3 mL/min using the following binary solvent system with A consisting of water with 0.1% formic acid additive and solvent B consisting of acetonitrile with 0.1% formic acid additive (LC/MS grade, Fisher Scientific).

    Injection:

    Article Title: Initial Clinical Experience with AZD5718, a Novel Once Daily Oral 5‐Lipoxygenase Activating Protein Inhibitor
    Article Snippet: .. The extracts were injected and analyzed by ultraperformance liquid‐chromatography tandem mass spectrometry (LC‐MS/MS) using an Acquity, BEH C18, 50 × 2.1 mm, particle size 1.7 μm column (Waters, Milford, MA) maintained at 40°C using a gradient of 20–98% acetonitrile in 10 mM ammonium formate (aq, 0.2% formic acid) as the mobile phase. ..

    Liquid Chromatography:

    Article Title: Biochemometrics to Identify Synergists and Additives from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal)
    Article Snippet: .. Extracts were suspended in 1:1 MeOH–dioxane (LC/MS grade, Fisher Scientific, Hampton, NH, USA) at either 1 or 0.1 mg/mL and subjected to ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis via a Waters Acquity UPLC with an Acquity UPLC column (BEH C18 , 1.7 μ m, 2.1 × 50 mm, Waters Corporation, Milford, MA, USA) and a Thermo Q-Exactive Plus orbitrap mass spectrometer with heated electrospray ionization (Thermo Fisher Scientific, Milford, MA, USA). .. Analysis was performed at a flow rate of 0.3 mL/min using the following binary solvent system with A consisting of water with 0.1% formic acid additive and solvent B consisting of acetonitrile with 0.1% formic acid additive (LC/MS grade, Fisher Scientific).

    Article Title: Initial Clinical Experience with AZD5718, a Novel Once Daily Oral 5‐Lipoxygenase Activating Protein Inhibitor
    Article Snippet: .. The extracts were injected and analyzed by ultraperformance liquid‐chromatography tandem mass spectrometry (LC‐MS/MS) using an Acquity, BEH C18, 50 × 2.1 mm, particle size 1.7 μm column (Waters, Milford, MA) maintained at 40°C using a gradient of 20–98% acetonitrile in 10 mM ammonium formate (aq, 0.2% formic acid) as the mobile phase. ..

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    Waters Corporation ultraperformance liquid chromatography tandem mass spectrometry
    LC-MS chromatograms (collected with <t>ultraperformance</t> liquid chromatography coupled to a Q-Exactive Orbitrap mass spectrometer) of a series of H. canadensis extract fractions. (A) Positive-ion electrospray mass spectrometry chromatogram of the crude extract after liquid–liquid partitioning. (B) The most active fraction (GS-4) of the crude extract shown in A after separation with flash chromatography over silica gel using a hexane–chloroform–methanol gradient (stage 1 separation). GS-4 was further fractionated using a second stage of flash chromatography over silica gel with a hexane–ethyl acetate–methanol gradient with GS-4-4 being the most active (C). GS-4-4-2 generated with stage 3 fractionation (using reversed-phase preparative HPLC with a water–acetonitrile gradient) (D). (E–H) The same H. canadensis extract and fractions analyzed using LC-MS in the negative-ion mode. Arrows represent the region of the chromatogram in which a given ion corresponding to a known constituent of H. canadensis (indicated by compound numbers) could be detected. Compounds 1 – 7 , 10 , 13 , 16 , 17 , 20 – 24 , and 28 while compounds 8 , 9 , 11 , 12 , 14 , 15 , 18 , 19 , and 25 – 27 29 is a new flavonoid that was isolated and identified based on NMR and MS data as part of this report. Red dotted arrows represent alkaloids and other compounds, while green dashed arrows represent flavonoids. In cases where a specific peak is not apparent in the chromatogram, the ion was identified based on mass spectrometric data.
    Ultraperformance Liquid Chromatography Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultraperformance liquid chromatography tandem mass spectrometry/product/Waters Corporation
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultraperformance liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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    LC-MS chromatograms (collected with ultraperformance liquid chromatography coupled to a Q-Exactive Orbitrap mass spectrometer) of a series of H. canadensis extract fractions. (A) Positive-ion electrospray mass spectrometry chromatogram of the crude extract after liquid–liquid partitioning. (B) The most active fraction (GS-4) of the crude extract shown in A after separation with flash chromatography over silica gel using a hexane–chloroform–methanol gradient (stage 1 separation). GS-4 was further fractionated using a second stage of flash chromatography over silica gel with a hexane–ethyl acetate–methanol gradient with GS-4-4 being the most active (C). GS-4-4-2 generated with stage 3 fractionation (using reversed-phase preparative HPLC with a water–acetonitrile gradient) (D). (E–H) The same H. canadensis extract and fractions analyzed using LC-MS in the negative-ion mode. Arrows represent the region of the chromatogram in which a given ion corresponding to a known constituent of H. canadensis (indicated by compound numbers) could be detected. Compounds 1 – 7 , 10 , 13 , 16 , 17 , 20 – 24 , and 28 while compounds 8 , 9 , 11 , 12 , 14 , 15 , 18 , 19 , and 25 – 27 29 is a new flavonoid that was isolated and identified based on NMR and MS data as part of this report. Red dotted arrows represent alkaloids and other compounds, while green dashed arrows represent flavonoids. In cases where a specific peak is not apparent in the chromatogram, the ion was identified based on mass spectrometric data.

    Journal: Journal of natural products

    Article Title: Biochemometrics to Identify Synergists and Additives from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal)

    doi: 10.1021/acs.jnatprod.7b00654

    Figure Lengend Snippet: LC-MS chromatograms (collected with ultraperformance liquid chromatography coupled to a Q-Exactive Orbitrap mass spectrometer) of a series of H. canadensis extract fractions. (A) Positive-ion electrospray mass spectrometry chromatogram of the crude extract after liquid–liquid partitioning. (B) The most active fraction (GS-4) of the crude extract shown in A after separation with flash chromatography over silica gel using a hexane–chloroform–methanol gradient (stage 1 separation). GS-4 was further fractionated using a second stage of flash chromatography over silica gel with a hexane–ethyl acetate–methanol gradient with GS-4-4 being the most active (C). GS-4-4-2 generated with stage 3 fractionation (using reversed-phase preparative HPLC with a water–acetonitrile gradient) (D). (E–H) The same H. canadensis extract and fractions analyzed using LC-MS in the negative-ion mode. Arrows represent the region of the chromatogram in which a given ion corresponding to a known constituent of H. canadensis (indicated by compound numbers) could be detected. Compounds 1 – 7 , 10 , 13 , 16 , 17 , 20 – 24 , and 28 while compounds 8 , 9 , 11 , 12 , 14 , 15 , 18 , 19 , and 25 – 27 29 is a new flavonoid that was isolated and identified based on NMR and MS data as part of this report. Red dotted arrows represent alkaloids and other compounds, while green dashed arrows represent flavonoids. In cases where a specific peak is not apparent in the chromatogram, the ion was identified based on mass spectrometric data.

    Article Snippet: Extracts were suspended in 1:1 MeOH–dioxane (LC/MS grade, Fisher Scientific, Hampton, NH, USA) at either 1 or 0.1 mg/mL and subjected to ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis via a Waters Acquity UPLC with an Acquity UPLC column (BEH C18 , 1.7 μ m, 2.1 × 50 mm, Waters Corporation, Milford, MA, USA) and a Thermo Q-Exactive Plus orbitrap mass spectrometer with heated electrospray ionization (Thermo Fisher Scientific, Milford, MA, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry, Chromatography, Generated, Fractionation, High Performance Liquid Chromatography, Isolation, Nuclear Magnetic Resonance