ultra rna library prep kit  (New England Biolabs)


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    Name:
    NEBNext Ultra RNA Library Prep Kit for Illumina
    Description:
    NEBNext Ultra RNA Library Prep Kit for Illumina 96 rxns
    Catalog Number:
    e7530l
    Price:
    3425
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
    Buy from Supplier


    Structured Review

    New England Biolabs ultra rna library prep kit
    NEBNext Ultra RNA Library Prep Kit for Illumina
    NEBNext Ultra RNA Library Prep Kit for Illumina 96 rxns
    https://www.bioz.com/result/ultra rna library prep kit/product/New England Biolabs
    Average 90 stars, based on 548 article reviews
    Price from $9.99 to $1999.99
    ultra rna library prep kit - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Transgenerational Effects of Extended Dauer Diapause on Starvation Survival and Gene Expression Plasticity in Caenorhabditis elegans"

    Article Title: Transgenerational Effects of Extended Dauer Diapause on Starvation Survival and Gene Expression Plasticity in Caenorhabditis elegans

    Journal: Genetics

    doi: 10.1534/genetics.118.301250

    mRNA-seq reveals relative contributions of current environment and ancestral environment in shaping gene expression variation. (A) Schematic of experimental set-up for collection of RNA-seq samples. (B) Principal component analysis (PCA) of six conditions including all 8649 reliably detected genes; 97% of variance explained by whether worms were fed or starved at collection (PC1), and 1.3% of variance explained by whether ancestors experienced control, short-term dauer, or long-term dauer conditions (PC2). Mean CPM of biological replicates were used for each condition for PCA. Number of biological replicates: control starved (12), ST dauer starved (9), LT dauer starved (6), control fed (4), ST dauer fed (4), and LT dauer fed (3).
    Figure Legend Snippet: mRNA-seq reveals relative contributions of current environment and ancestral environment in shaping gene expression variation. (A) Schematic of experimental set-up for collection of RNA-seq samples. (B) Principal component analysis (PCA) of six conditions including all 8649 reliably detected genes; 97% of variance explained by whether worms were fed or starved at collection (PC1), and 1.3% of variance explained by whether ancestors experienced control, short-term dauer, or long-term dauer conditions (PC2). Mean CPM of biological replicates were used for each condition for PCA. Number of biological replicates: control starved (12), ST dauer starved (9), LT dauer starved (6), control fed (4), ST dauer fed (4), and LT dauer fed (3).

    Techniques Used: Expressing, RNA Sequencing Assay

    2) Product Images from "RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3"

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3

    Journal: Molecular Cancer

    doi: 10.1186/s12943-019-1004-4

    Epigenetic silencing of BNIP3 by an FTO-m6A-dependent mechanism. a - b BNIP3 expression was significantly up-regulated in both RNA and protein expression level in stable FTO-knockdown MDA-MB-231 cells ( a ) and MCF-7 cells ( b ). ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. c , d Knockdown of FTO promoted the cleavage of Caaspase 3 and decreased Bcl2 in MDA-MB-231 cells ( c ) and MCF-7 cells ( d ). e Knockdown of FTO promoted the m6A methylation in BNIP3 mRNA by the m6A MeRIP analysis. * P ≤ 0.05. f Wild-type or m6A consensus sequence mutant BNIP3 3’UTR was fused with firefly luciferase reporter. Mutation of m6A consensus sequences were generated by replacing adenosine with thymine. g Relative luciferase activity of the wild-type and 3 mutant BNIP3 3’UTR reporter vectors in FTO-knockdown MDA-MB-231 cells
    Figure Legend Snippet: Epigenetic silencing of BNIP3 by an FTO-m6A-dependent mechanism. a - b BNIP3 expression was significantly up-regulated in both RNA and protein expression level in stable FTO-knockdown MDA-MB-231 cells ( a ) and MCF-7 cells ( b ). ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. c , d Knockdown of FTO promoted the cleavage of Caaspase 3 and decreased Bcl2 in MDA-MB-231 cells ( c ) and MCF-7 cells ( d ). e Knockdown of FTO promoted the m6A methylation in BNIP3 mRNA by the m6A MeRIP analysis. * P ≤ 0.05. f Wild-type or m6A consensus sequence mutant BNIP3 3’UTR was fused with firefly luciferase reporter. Mutation of m6A consensus sequences were generated by replacing adenosine with thymine. g Relative luciferase activity of the wild-type and 3 mutant BNIP3 3’UTR reporter vectors in FTO-knockdown MDA-MB-231 cells

    Techniques Used: Expressing, Multiple Displacement Amplification, Methylation, Sequencing, Mutagenesis, Luciferase, Generated, Activity Assay

    Up-regulation of FTO RNA demethylase in human breast cancer. a Heat map diagram of differential gene expression in breast tumors and normal tissues. b Expression of the m6A regulatory enzymes in primary human breast tumors. ** P ≤ 0.01, *** P ≤ 0.001. c Relative FTO mRNA expression level in molecular subtypes and clinical stages of breast tumors. NORM: normal tissues; TNBC: ER−/PR−/Her2-; DNBC: ER−/PR−/Her2+; TPBC: ER+/PR+/Her2+. ** P ≤ 0.01, **** P ≤ 0.0001. d Higher levels of FTO in human breast cancer tissues in comparison with normal breast tissues by immunohistochemistry assay. e FTO up-regulation was quantified from the immunohistochemistry results. f The global mRNA m6A level in human breast cancer samples determined by RNA m6A dot-blotting assay. g The global mRNA m6A level in human breast cancer samples determined by RNA m6A colorimetric analysis. * P ≤ 0.05. h FTO up-regulation was significantly associated with shorter overall survival in patients with advanced stage of breast cancer. i FTO up-regulation was significantly associated with shorter overall survival in patients with ER negative breast cancer
    Figure Legend Snippet: Up-regulation of FTO RNA demethylase in human breast cancer. a Heat map diagram of differential gene expression in breast tumors and normal tissues. b Expression of the m6A regulatory enzymes in primary human breast tumors. ** P ≤ 0.01, *** P ≤ 0.001. c Relative FTO mRNA expression level in molecular subtypes and clinical stages of breast tumors. NORM: normal tissues; TNBC: ER−/PR−/Her2-; DNBC: ER−/PR−/Her2+; TPBC: ER+/PR+/Her2+. ** P ≤ 0.01, **** P ≤ 0.0001. d Higher levels of FTO in human breast cancer tissues in comparison with normal breast tissues by immunohistochemistry assay. e FTO up-regulation was quantified from the immunohistochemistry results. f The global mRNA m6A level in human breast cancer samples determined by RNA m6A dot-blotting assay. g The global mRNA m6A level in human breast cancer samples determined by RNA m6A colorimetric analysis. * P ≤ 0.05. h FTO up-regulation was significantly associated with shorter overall survival in patients with advanced stage of breast cancer. i FTO up-regulation was significantly associated with shorter overall survival in patients with ER negative breast cancer

    Techniques Used: Expressing, Immunohistochemistry

    RNA-Seq and m6A-Seq identified BNIP3 as a downstream target of FTO-mediated m6A modification. a Venn diagram illustrated overlap in differentially expressed genes in FTO-knockdown MDA-MB-231 cells and MCF-4 cells treated with DMOG. b KEGG analysis shows that FTO-knockdown regulate pathways involved in cell proliferation, cell cycle and apoptosis. c m6A-Seq identification of m6A modification in BNIP3 mRNA near to the YTHDF2 binding sites. d Differentially expressed genes by inhibiting or knockdown of FTO involved in the FoxO signaling pathway. Red color indicates up-regulated genes, while purple color indicates down-regulated genes. e , f Heatmap of up-regulated genes in FTO-knockdown MDA-MB-231 cells ( e ) and MCF-4 cells treated with DMOG ( f ). g Co-expression analysis of BNIP3 by the string
    Figure Legend Snippet: RNA-Seq and m6A-Seq identified BNIP3 as a downstream target of FTO-mediated m6A modification. a Venn diagram illustrated overlap in differentially expressed genes in FTO-knockdown MDA-MB-231 cells and MCF-4 cells treated with DMOG. b KEGG analysis shows that FTO-knockdown regulate pathways involved in cell proliferation, cell cycle and apoptosis. c m6A-Seq identification of m6A modification in BNIP3 mRNA near to the YTHDF2 binding sites. d Differentially expressed genes by inhibiting or knockdown of FTO involved in the FoxO signaling pathway. Red color indicates up-regulated genes, while purple color indicates down-regulated genes. e , f Heatmap of up-regulated genes in FTO-knockdown MDA-MB-231 cells ( e ) and MCF-4 cells treated with DMOG ( f ). g Co-expression analysis of BNIP3 by the string

    Techniques Used: RNA Sequencing Assay, Modification, Multiple Displacement Amplification, Binding Assay, Expressing

    3) Product Images from "Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice"

    Article Title: Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice

    Journal: Life Science Alliance

    doi: 10.26508/lsa.201800195

    Gene expression signature of NK cells in NSG hL-7xhIL-15 humanized mice. RNA sequencing was performed using RNA extracted from hCD56+ splenic NK cells of conventional NSG (NSG, n = 2) and hIL-7xhIL-15 KI NSG (hIL7xhIL-15 KI NSG, n = 6) humanized mice. (A) Differentially expressing genes are shown. Gene expression profiles of human NK cells of humanized mice were compared with those from NK cells recovered from human PBMCs. (B) Expression of genes related to cytokine and cytotoxicity are shown.
    Figure Legend Snippet: Gene expression signature of NK cells in NSG hL-7xhIL-15 humanized mice. RNA sequencing was performed using RNA extracted from hCD56+ splenic NK cells of conventional NSG (NSG, n = 2) and hIL-7xhIL-15 KI NSG (hIL7xhIL-15 KI NSG, n = 6) humanized mice. (A) Differentially expressing genes are shown. Gene expression profiles of human NK cells of humanized mice were compared with those from NK cells recovered from human PBMCs. (B) Expression of genes related to cytokine and cytotoxicity are shown.

    Techniques Used: Expressing, Mouse Assay, RNA Sequencing Assay

    Related Articles

    Amplification:

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7530). .. After Not I digestion, the library DNA was purified for PCR amplification (16 cycles).

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: We produced cDNA libraries from isolated mRNA using magnetic bead isolation of mRNA followed by cDNA synthesis and PCR amplification. .. Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations.

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    Article Title: Potential anti-cancer effect of curcumin in human lung squamous cell carcinoma
    Article Snippet: RNA libraries were prepared according to the manufacturer's recommended protocol for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530). .. The cDNA were then amplified by 12 cycles of polymerase chain reaction with New England BioLabs (NEB) Phusion polymerase (Ipswich, MA, USA) and purified to obtain RNA libraries.

    Construct:

    Article Title: seq-ImmuCC: Cell-Centric View of Tissue Transcriptome Measuring Cellular Compositions of Immune Microenvironment From Mouse RNA-Seq Data
    Article Snippet: Briefly, RNA-Seq libraries were constructed after rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB). .. The E6310L NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530S) (NEB) was used according to the manufacturer’s instructions and the cDNAs were sequenced with the Hiseq X10 platform (Illumina).

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: .. The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7530). .. After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion.

    Electrophoresis:

    Article Title: Comparative genome analysis indicates high evolutionary potential of pathogenicity genes in Colletotrichum tanaceti
    Article Snippet: Contaminating genomic DNA was removed from RNA samples by Ambion DNase I (Thermo Fisher Scientific, USA) treatment; the integrity and quantity of total RNA was confirmed by 1% agarose gel electrophoresis and the Experion automated electrophoresis system (Biorad Laboratories, Australia). .. RNA libaries were prepared using both E7530L and E & 335L NEBNext Ultra RNA Library Prep Kits (New England Biolabs, USA) to generate fragment sizes of 351–371 bp.

    Incubation:

    Article Title: Comparative genome analysis indicates high evolutionary potential of pathogenicity genes in Colletotrichum tanaceti
    Article Snippet: Each petri dish was sealed with parafilm and incubated at 24°C with a 12 h-photoperiod. .. RNA libaries were prepared using both E7530L and E & 335L NEBNext Ultra RNA Library Prep Kits (New England Biolabs, USA) to generate fragment sizes of 351–371 bp.

    Article Title: Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families
    Article Snippet: We added 200 μL of 20% chloroform, shook the solution, and centrifuged for 20 min after three minutes of incubation to separate the RNA. .. We used the New England Biolabs (NEB, Ipswich, MA, USA) NEBNext Poly(A) mRNA magnetic isolation kit (E7490S), the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530), and the NEBNext Multiplex Oligos for Illumina (E7335 -Index primer set 1) following the manufacturer’s protocols with a target mean insert size of 370 bp, a fragmentation time of 15.5 min, and 14 PCR cycles in the final enrichment step to yield the appropriate cDNA concentration.

    Expressing:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: The composition of encapsulated RNA was evaluated by performing comprehensive RNAseq on total RNA from producer cells (representing expression levels) and nucleocapsids (representing encapsulated RNA). .. The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S).

    Article Title: Whole-exome sequencing identified a homozygous BRDT mutation in a patient with acephalic spermatozoa
    Article Snippet: Briefly, after RNA quality examination, library preparation and construction was performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA), followed by library examination, library clustering and sequencing on the Illumina Hiseq 4000 platform. .. To assess the cell populations of all the RNA-Seq samples, the Pearson coefficients were computed using all genes expressed in more than two samples, and hierarchical clustering was performed using the hclust function, and “ward.D2” method in R. Principal Component Analysis (PCA) was performed using the same criterion with the prcomp function in R. Differential gene expression analysis was performed by using R Bioconductor, DESeq2 package, as previously described [ ].

    Ligation:

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7530). .. After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion.

    Generated:

    Article Title: Perturbation of IIS/TOR signaling alters the landscape of sex-differential gene expression in Drosophila
    Article Snippet: RNA-seq libraries were generated using 2 μg total RNA. .. The NEBNext Ultra RNA Library Prep Kit for Illumina, with the polyA purification module (New England Biolabs Inc. E7530L), was used for Illumina library generation.

    Article Title: Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation
    Article Snippet: Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacturer protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490). .. The RNAseq data was generated on NextSeq500 mid-output (75 bp paired-end reads) kit v2 (Illumina, FC-404-2005).

    other:

    Article Title: Overrepresentation Analyses of Differentially Expressed Genes in the Smut Fungus Ustilago bromivora during Saprophytic and in planta Growth
    Article Snippet: Pipetman Diamond tips, D200 (Gilson, catalog number: F161931) Pipetman Diamond tips, D1000 (Gilson, catalog number: F161671) 50 ml sterile disposable vial (SARSTEDT, catalog number: 62.547.254) 1.5 ml microcentrifuge tubes (SARSTEDT, catalog number: 72.690.001) Micro-homogenizer (Carl Roth, catalog number: K994.1) Glycerol anhydrous (Applichem, catalog number: A1123,1000) Liquid nitrogen Sodium hypochlorite ~10% (Honeywell International, catalog number: 71696-2.5L) Hydrochloric acid (HCl) 37% (Applichem, catalog number: 131020.1211) TRIzol reagent (Thermo Fisher Scientific, Invitrogen™, catalog number: 15596026) TURBO DNA- free Kit (Thermo Fisher Scientific, Invitrogen™, catalog number: AM1907) Ribo-Zero rRNA Removal Kit (Plant) (Illumina, catalog number: MRZPL1224) NEB Next Ultra RNA Library Prep Kit (New England Biolabs, catalog number: E7530S) Ampure XP beads (Beckman Coulter, catalog number: ) Agilent RNA 6000 Nano Kit for Bioanalyzer (Agilent Technologies, catalog number: 5067-1511) or Standard Sensitivity RNA Analysis Kit (15 nt) (Advanced Analytical Technologies, catalog number: DNF-471) Illumina PE Cluster Kit (Illumina, catalog number: FC-401-4003) Illumina 250 cycle SBS reagents (Illumina, catalog number: PE-401-4001) Potato dextrose broth (BD, catalog number: 254920) Standard potting soil (Topfsubstrat ED63, Einheitserde, catalog number: SP ED63 T, obtained from GBC-Gartenbauzentrum Schwechat, catalog number: 013224) Perlite (Perlite Premium 2-6 mm, Gramoflor, obtained from GBC-Gartenbauzentrum Schwechat, catalog number: 079568) Silica sand (Quarzsand 0.5-2 mm, min2C, obtained from GBC-Gartenbauzentrum Schwechat, catalog number: 005989) Germination soil (Aussaaterde, Huminsubstrat N3, Neuhaus, Klasmann-Deilmann, obtained from GBC-Gartenbauzentrum Schwechat, catalog number: 001318) Potato dextrose liquid medium (PD medium) (see ) Soil mix (see )

    Polymerase Chain Reaction:

    Article Title: Transgenerational Effects of Extended Dauer Diapause on Starvation Survival and Gene Expression Plasticity in Caenorhabditis elegans
    Article Snippet: .. RNA was isolated using TRIzol (Invitrogen) using the manufacturer’s protocol with minor modifications; 1 ml of TRIzol was used per sample along with 100 μl of acid-washed sand. mRNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) in two batches, utilizing either 500 or 100 ng of starting RNA per library and 12 or 15 PCR cycles, respectively. .. Libraries were sequenced using Illumina HiSeq 4000 to obtain single-end 50 bp reads.

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
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    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
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    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    Article Title: Potential anti-cancer effect of curcumin in human lung squamous cell carcinoma
    Article Snippet: RNA libraries were prepared according to the manufacturer's recommended protocol for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530). .. The cDNA were then amplified by 12 cycles of polymerase chain reaction with New England BioLabs (NEB) Phusion polymerase (Ipswich, MA, USA) and purified to obtain RNA libraries.

    Article Title: Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families
    Article Snippet: .. We used the New England Biolabs (NEB, Ipswich, MA, USA) NEBNext Poly(A) mRNA magnetic isolation kit (E7490S), the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530), and the NEBNext Multiplex Oligos for Illumina (E7335 -Index primer set 1) following the manufacturer’s protocols with a target mean insert size of 370 bp, a fragmentation time of 15.5 min, and 14 PCR cycles in the final enrichment step to yield the appropriate cDNA concentration. .. To do this, we isolated RNA with poly-A tails from the total RNA with oligo-dT beads and then immediately moved to first and second strand cDNA synthesis [ ].

    Molecular Cloning:

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations. .. The total amplifiable concentration of cDNA in each library was then determined using KAPA qPCR at the Florida State University Molecular Cloning Facility.

    RNA Sequencing Assay:

    Article Title: seq-ImmuCC: Cell-Centric View of Tissue Transcriptome Measuring Cellular Compositions of Immune Microenvironment From Mouse RNA-Seq Data
    Article Snippet: Paragraph title: RNA-Seq Library Preparation ... The E6310L NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530S) (NEB) was used according to the manufacturer’s instructions and the cDNAs were sequenced with the Hiseq X10 platform (Illumina).

    Article Title: Whole-exome sequencing identified a homozygous BRDT mutation in a patient with acephalic spermatozoa
    Article Snippet: Paragraph title: RNA-sequencing and data analysis ... Briefly, after RNA quality examination, library preparation and construction was performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA), followed by library examination, library clustering and sequencing on the Illumina Hiseq 4000 platform.

    Article Title: Regulatory chromatin landscape in Arabidopsis thaliana roots uncovered by coupling INTACT and ATAC-seq
    Article Snippet: Paragraph title: RNA-seq ... Libraries were prepared with the NEBNext RNA library prep kit (module E7530-E7490). cDNA was quantified using a Qubit HS DNA kit (Thermo Fisher, Q32854) and analyzed on a BioAnalyzer or TapeStation.

    Article Title: Potential anti-cancer effect of curcumin in human lung squamous cell carcinoma
    Article Snippet: Paragraph title: Cell treatment and ribonucleic acid sequencing (RNA-seq) ... RNA libraries were prepared according to the manufacturer's recommended protocol for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530).

    Article Title: Perturbation of IIS/TOR signaling alters the landscape of sex-differential gene expression in Drosophila
    Article Snippet: RNA-seq libraries were generated using 2 μg total RNA. .. The NEBNext Ultra RNA Library Prep Kit for Illumina, with the polyA purification module (New England Biolabs Inc. E7530L), was used for Illumina library generation.

    Article Title: β-Catenin directs the transformation of testis Sertoli cells to ovarian granulosa-like cells by inducing Foxl2 expression
    Article Snippet: Paragraph title: RNA sequencing analysis ... The main reagents and instruments used for RNA library construction were from the New England Biolabs Next Ultra RNA Library Prep Kit (New England Biolabs, E7530L).

    RNA HS Assay:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    Isolation:

    Article Title: Transgenerational Effects of Extended Dauer Diapause on Starvation Survival and Gene Expression Plasticity in Caenorhabditis elegans
    Article Snippet: .. RNA was isolated using TRIzol (Invitrogen) using the manufacturer’s protocol with minor modifications; 1 ml of TRIzol was used per sample along with 100 μl of acid-washed sand. mRNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) in two batches, utilizing either 500 or 100 ng of starting RNA per library and 12 or 15 PCR cycles, respectively. .. Libraries were sequenced using Illumina HiSeq 4000 to obtain single-end 50 bp reads.

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: .. Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations. .. We used a fragmentation time of 13 minutes, 30 seconds to achieve a target mean fragment size of 400 bp, and 14 PCR cycles for amplification of double stranded cDNA libraries.

    Article Title: Regulatory chromatin landscape in Arabidopsis thaliana roots uncovered by coupling INTACT and ATAC-seq
    Article Snippet: RNA-seq RNA was isolated from 14-day-old roots using TRIZOL (Life Technologies) reagent and cleaned with RNA Clean & Concentrator™-5 (Zymo, R1015). .. Libraries were prepared with the NEBNext RNA library prep kit (module E7530-E7490). cDNA was quantified using a Qubit HS DNA kit (Thermo Fisher, Q32854) and analyzed on a BioAnalyzer or TapeStation.

    Article Title: Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families
    Article Snippet: .. We used the New England Biolabs (NEB, Ipswich, MA, USA) NEBNext Poly(A) mRNA magnetic isolation kit (E7490S), the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530), and the NEBNext Multiplex Oligos for Illumina (E7335 -Index primer set 1) following the manufacturer’s protocols with a target mean insert size of 370 bp, a fragmentation time of 15.5 min, and 14 PCR cycles in the final enrichment step to yield the appropriate cDNA concentration. .. To do this, we isolated RNA with poly-A tails from the total RNA with oligo-dT beads and then immediately moved to first and second strand cDNA synthesis [ ].

    Article Title: Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation
    Article Snippet: .. Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacturer protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490). .. The RNAseq data was generated on NextSeq500 mid-output (75 bp paired-end reads) kit v2 (Illumina, FC-404-2005).

    Article Title: β-Catenin directs the transformation of testis Sertoli cells to ovarian granulosa-like cells by inducing Foxl2 expression
    Article Snippet: Total RNA was extracted from isolated cells, including control granulosa cells, control Sertoli cells, and Ctnnb1- overactivated Sertoli cells from E16.5 embryos using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer's instructions. .. The main reagents and instruments used for RNA library construction were from the New England Biolabs Next Ultra RNA Library Prep Kit (New England Biolabs, E7530L).

    RNA Extraction:

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: Paragraph title: RNA extraction and sequencing ... Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations.

    Article Title: Potential anti-cancer effect of curcumin in human lung squamous cell carcinoma
    Article Snippet: After the treatment period, attached cells were harvested for RNA extraction following the manufacturer's protocol using an RNeasy kit (Invitrogen, Carlsbad, CA, USA). .. RNA libraries were prepared according to the manufacturer's recommended protocol for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530).

    Article Title: Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation
    Article Snippet: Paragraph title: RNA extraction, library construction and data generation and analysis ... Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacturer protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490).

    Purification:

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7530). .. After Not I digestion, the library DNA was purified for PCR amplification (16 cycles).

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: Once cells were lysed, total RNA was isolated using chloroform and purified via isopropyl alcohol and ethanol precipitation. .. Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations.

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    Article Title: Potential anti-cancer effect of curcumin in human lung squamous cell carcinoma
    Article Snippet: RNA libraries were prepared according to the manufacturer's recommended protocol for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530). .. The cDNA were then amplified by 12 cycles of polymerase chain reaction with New England BioLabs (NEB) Phusion polymerase (Ipswich, MA, USA) and purified to obtain RNA libraries.

    Article Title: Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families
    Article Snippet: We used the New England Biolabs (NEB, Ipswich, MA, USA) NEBNext Poly(A) mRNA magnetic isolation kit (E7490S), the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530), and the NEBNext Multiplex Oligos for Illumina (E7335 -Index primer set 1) following the manufacturer’s protocols with a target mean insert size of 370 bp, a fragmentation time of 15.5 min, and 14 PCR cycles in the final enrichment step to yield the appropriate cDNA concentration. .. We used Agencourt AMPure XP PCR Purification Beads (Beckman Coulter) to purify DNA throughout the protocol.

    Article Title: Perturbation of IIS/TOR signaling alters the landscape of sex-differential gene expression in Drosophila
    Article Snippet: .. The NEBNext Ultra RNA Library Prep Kit for Illumina, with the polyA purification module (New England Biolabs Inc. E7530L), was used for Illumina library generation. .. Pooled, barcoded, libraries were sequenced on an Illumina HiSeq 2500 (100 BP, single end).

    Sequencing:

    Article Title: Holo-Seq: single-cell sequencing of holo-transcriptome
    Article Snippet: .. The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7530). .. After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion.

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: Paragraph title: RNA extraction and sequencing ... Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations.

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: Paragraph title: Illumina sequencing sample preparation for comprehensive RNAseq ... The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S).

    Article Title: Whole-exome sequencing identified a homozygous BRDT mutation in a patient with acephalic spermatozoa
    Article Snippet: .. Briefly, after RNA quality examination, library preparation and construction was performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA), followed by library examination, library clustering and sequencing on the Illumina Hiseq 4000 platform. ..

    Article Title: Potential anti-cancer effect of curcumin in human lung squamous cell carcinoma
    Article Snippet: Paragraph title: Cell treatment and ribonucleic acid sequencing (RNA-seq) ... RNA libraries were prepared according to the manufacturer's recommended protocol for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530).

    Article Title: Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families
    Article Snippet: Paragraph title: 5.4. Venom Gland Transcriptome Sequencing ... We used the New England Biolabs (NEB, Ipswich, MA, USA) NEBNext Poly(A) mRNA magnetic isolation kit (E7490S), the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530), and the NEBNext Multiplex Oligos for Illumina (E7335 -Index primer set 1) following the manufacturer’s protocols with a target mean insert size of 370 bp, a fragmentation time of 15.5 min, and 14 PCR cycles in the final enrichment step to yield the appropriate cDNA concentration.

    cDNA Library Assay:

    Article Title: Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families
    Article Snippet: We used 1 μg of total RNA for mRNA isolation and cDNA library preparation [ , ]. .. We used the New England Biolabs (NEB, Ipswich, MA, USA) NEBNext Poly(A) mRNA magnetic isolation kit (E7490S), the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530), and the NEBNext Multiplex Oligos for Illumina (E7335 -Index primer set 1) following the manufacturer’s protocols with a target mean insert size of 370 bp, a fragmentation time of 15.5 min, and 14 PCR cycles in the final enrichment step to yield the appropriate cDNA concentration.

    Agarose Gel Electrophoresis:

    Article Title: Potential anti-cancer effect of curcumin in human lung squamous cell carcinoma
    Article Snippet: RNA was properly tested for quality on 2% agarose gel electrophoresis and spectrophotometer before RNA-seq was conducted using an Illumina HiSeq 2000 Genome Analyzer (San Diego, CA, USA). .. RNA libraries were prepared according to the manufacturer's recommended protocol for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530).

    Article Title: Comparative genome analysis indicates high evolutionary potential of pathogenicity genes in Colletotrichum tanaceti
    Article Snippet: Contaminating genomic DNA was removed from RNA samples by Ambion DNase I (Thermo Fisher Scientific, USA) treatment; the integrity and quantity of total RNA was confirmed by 1% agarose gel electrophoresis and the Experion automated electrophoresis system (Biorad Laboratories, Australia). .. RNA libaries were prepared using both E7530L and E & 335L NEBNext Ultra RNA Library Prep Kits (New England Biolabs, USA) to generate fragment sizes of 351–371 bp.

    Real-time Polymerase Chain Reaction:

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations. .. The total amplifiable concentration of cDNA in each library was then determined using KAPA qPCR at the Florida State University Molecular Cloning Facility.

    Multiplex Assay:

    Article Title: Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families
    Article Snippet: .. We used the New England Biolabs (NEB, Ipswich, MA, USA) NEBNext Poly(A) mRNA magnetic isolation kit (E7490S), the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530), and the NEBNext Multiplex Oligos for Illumina (E7335 -Index primer set 1) following the manufacturer’s protocols with a target mean insert size of 370 bp, a fragmentation time of 15.5 min, and 14 PCR cycles in the final enrichment step to yield the appropriate cDNA concentration. .. To do this, we isolated RNA with poly-A tails from the total RNA with oligo-dT beads and then immediately moved to first and second strand cDNA synthesis [ ].

    Sample Prep:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: Paragraph title: Illumina sequencing sample preparation for comprehensive RNAseq ... The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S).

    Ethanol Precipitation:

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: Once cells were lysed, total RNA was isolated using chloroform and purified via isopropyl alcohol and ethanol precipitation. .. Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations.

    Spectrophotometry:

    Article Title: Potential anti-cancer effect of curcumin in human lung squamous cell carcinoma
    Article Snippet: RNA was properly tested for quality on 2% agarose gel electrophoresis and spectrophotometer before RNA-seq was conducted using an Illumina HiSeq 2000 Genome Analyzer (San Diego, CA, USA). .. RNA libraries were prepared according to the manufacturer's recommended protocol for the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530).

    Produced:

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: We produced cDNA libraries from isolated mRNA using magnetic bead isolation of mRNA followed by cDNA synthesis and PCR amplification. .. Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations.

    Article Title: Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation
    Article Snippet: .. Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacturer protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490). .. The RNAseq data was generated on NextSeq500 mid-output (75 bp paired-end reads) kit v2 (Illumina, FC-404-2005).

    Concentration Assay:

    Article Title: Comparative venom-gland transcriptomics and venom proteomics of four Sidewinder Rattlesnake (Crotalus cerastes) lineages reveal little differential expression despite individual variation
    Article Snippet: Following bead isolation and cleanup, cDNA libraries were prepared from isolated mRNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB #E7530) following manufacturer’s recommendations. .. The total amplifiable concentration of cDNA in each library was then determined using KAPA qPCR at the Florida State University Molecular Cloning Facility.

    Article Title: Phenotypic Variation in Mojave Rattlesnake (Crotalus scutulatus) Venom Is Driven by Four Toxin Families
    Article Snippet: .. We used the New England Biolabs (NEB, Ipswich, MA, USA) NEBNext Poly(A) mRNA magnetic isolation kit (E7490S), the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530), and the NEBNext Multiplex Oligos for Illumina (E7335 -Index primer set 1) following the manufacturer’s protocols with a target mean insert size of 370 bp, a fragmentation time of 15.5 min, and 14 PCR cycles in the final enrichment step to yield the appropriate cDNA concentration. .. To do this, we isolated RNA with poly-A tails from the total RNA with oligo-dT beads and then immediately moved to first and second strand cDNA synthesis [ ].

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    New England Biolabs nebnext kit
    Nebnext Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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