ultra rna library kit  (New England Biolabs)


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  • 99
    Name:
    NEBNext Ultra RNA Library Prep Kit for Illumina
    Description:
    NEBNext Ultra RNA Library Prep Kit for Illumina 96 rxns
    Catalog Number:
    e7530l
    Price:
    3494
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
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    New England Biolabs ultra rna library kit
    NEBNext Ultra RNA Library Prep Kit for Illumina
    NEBNext Ultra RNA Library Prep Kit for Illumina 96 rxns
    https://www.bioz.com/result/ultra rna library kit/product/New England Biolabs
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    ultra rna library kit - by Bioz Stars, 2020-04
    99/100 stars

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    1) Product Images from "Whole transcriptome profiling reveals the RNA content of motor axons"

    Article Title: Whole transcriptome profiling reveals the RNA content of motor axons

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkv1027

    Whole transcriptome profiling of serially diluted Human Brain Reference RNA with ERCC control RNAs. ( A ) Amplification products for 5 ng, 500 pg, 50 pg, 10 pg and 0 pg input RNA. The number of PCR cycles is indicated. ( B ) qPCR for Gapdh on pre-amplified cDNA (0 cycles) and on purified PCR products. Data are mean crossing points with standard deviation. Ct, crossing point. ( C ) Libraries for high-throughput sequencing. L, pooled libraries. ( D ) Correlation analysis of technical replicates for genes with FPKM ≥ 0.001. Pearson r and Spearman rs correlation coefficients are indicated. ( E ) Correlation analysis of ERCC control RNAs. Pearson r and Spearman rs correlation coefficients of the FPKM values are shown for each comparison. ( F ) Scatter plots showing the FPKM level of each ERCC control RNA relative to its calculated number of molecules.
    Figure Legend Snippet: Whole transcriptome profiling of serially diluted Human Brain Reference RNA with ERCC control RNAs. ( A ) Amplification products for 5 ng, 500 pg, 50 pg, 10 pg and 0 pg input RNA. The number of PCR cycles is indicated. ( B ) qPCR for Gapdh on pre-amplified cDNA (0 cycles) and on purified PCR products. Data are mean crossing points with standard deviation. Ct, crossing point. ( C ) Libraries for high-throughput sequencing. L, pooled libraries. ( D ) Correlation analysis of technical replicates for genes with FPKM ≥ 0.001. Pearson r and Spearman rs correlation coefficients are indicated. ( E ) Correlation analysis of ERCC control RNAs. Pearson r and Spearman rs correlation coefficients of the FPKM values are shown for each comparison. ( F ) Scatter plots showing the FPKM level of each ERCC control RNA relative to its calculated number of molecules.

    Techniques Used: Amplification, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Purification, Standard Deviation, Next-Generation Sequencing

    Related Articles

    Amplification:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    High Throughput Screening Assay:

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000. ..

    Construct:

    Article Title: Transcriptomic Analysis of Differentially Expressed Genes during Flower Organ Development in Genetic Male Sterile and Male Fertile Tagetes erecta by Digital Gene-Expression Profiling
    Article Snippet: Library preparation and sequencing RNA Samples were sent to Novogene Bioinformatics Technology Co. Ltd (Beijing), where the libraries were constructed and sequenced using Illumina HiSeq 2000 platform. .. Sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s protocols and index codes were added to attribute sequences to each sample.

    End-sequence Profiling:

    Article Title: The primary transcriptome, small RNAs and regulation of antimicrobial resistance in Acinetobacter baumannii ATCC 17978
    Article Snippet: .. Libraries of the ESP RNA samples were prepared using the NEBNext™ Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions. .. These libraries were sequenced on an Illumina MiSeq (paired-end reads).

    Incubation:

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: Chemically fragmented RNA (100 nucleotides) was incubated with m6A antibody for immunoprecipitation according to the standard protocol of Magna methylated RNA immune-precipitation (MeRIP) m6A Kit (#17–10,499, Merck Millipore, USA). .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000.

    Expressing:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: The composition of encapsulated RNA was evaluated by performing comprehensive RNAseq on total RNA from producer cells (representing expression levels) and nucleocapsids (representing encapsulated RNA). .. The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S).

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: To obtain mRNA and miRNA expression profiles, total RNA of the liver (100 mg) was extracted using TRIzol reagent (Invitrogen, USA). .. Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions.

    Article Title: Rare cell variability and drug-induced reprogramming as a mode of cancer drug resistance
    Article Snippet: We used the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina to extract polyadenylated RNA and prepare barcoded RNA sequencing libraries. .. We then used R to perform differential expression analysis with DESeq2 (code available at https://bitbucket.org/arjunrajlaboratory/rajlabseqtools ) to identify resistance marker genes.

    Generated:

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: .. Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions. .. Then, the final purified libraries were evaluated using a BioAnalyzer 2100 and quantified.

    Article Title: Transcriptomic Analysis of Differentially Expressed Genes during Flower Organ Development in Genetic Male Sterile and Male Fertile Tagetes erecta by Digital Gene-Expression Profiling
    Article Snippet: .. Sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s protocols and index codes were added to attribute sequences to each sample. ..

    Article Title: Effects of anticholinergic agent on miRNA profiles and transcriptomes in a murine model of allergic rhinitis
    Article Snippet: .. mRNA library preparation for transcriptome sequencing and data analysis The mRNA libraries were generated using NEBNext® Ultra™ RNA Library Prep kit for Illumina® (New England BioLabs, Inc., Ipswich, MA, USA) according to the manufacturer's instructions. ..

    Article Title: Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation
    Article Snippet: Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacturer protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490). .. The RNAseq data was generated on NextSeq500 mid-output (75 bp paired-end reads) kit v2 (Illumina, FC-404-2005).

    Polymerase Chain Reaction:

    Article Title: Transgenerational Effects of Extended Dauer Diapause on Starvation Survival and Gene Expression Plasticity in Caenorhabditis elegans
    Article Snippet: .. RNA was isolated using TRIzol (Invitrogen) using the manufacturer’s protocol with minor modifications; 1 ml of TRIzol was used per sample along with 100 μl of acid-washed sand. mRNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) in two batches, utilizing either 500 or 100 ng of starting RNA per library and 12 or 15 PCR cycles, respectively. .. Libraries were sequenced using Illumina HiSeq 4000 to obtain single-end 50 bp reads.

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    RNA Sequencing Assay:

    Article Title: Rare cell detection by single cell RNA sequencing as guided by single molecule RNA FISH
    Article Snippet: Paragraph title: Bulk RNA sequencing ... We sequenced mRNA in bulk from WM989-A6 populations as per Shaffer et. al. We isolated mRNA and built sequencing libraries using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina.

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: Enrichment of m6A containing mRNA was analyzed either by qRT-PCR with the primers listed in Additional file : Table S1 or by high-throughput RNA sequencing. .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000.

    Article Title: Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting
    Article Snippet: .. For RNA-seq of C1R-CD1d parental cells, the same procedures were followed, except for the fact that libraries were prepared .using the NEBNext poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra RNA Library Prep kit for Illumina (New Engand Biolabs) and sequenced on an Illumina HiSeq 2500 platform to obtain paired-end 100bp reads. .. Reads obtained from parental C1R-CD1d RNA-seq experiments were aligned using TopHat (v2.0.14) ( ) against the Hg19 human reference genome using the default settings.

    Article Title: Rare cell variability and drug-induced reprogramming as a mode of cancer drug resistance
    Article Snippet: .. We used the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina to extract polyadenylated RNA and prepare barcoded RNA sequencing libraries. ..

    RNA HS Assay:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    Methylation:

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: Chemically fragmented RNA (100 nucleotides) was incubated with m6A antibody for immunoprecipitation according to the standard protocol of Magna methylated RNA immune-precipitation (MeRIP) m6A Kit (#17–10,499, Merck Millipore, USA). .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000.

    Multiple Displacement Amplification:

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: RNA m6A sequence and m6A-RNA immunoprecipitation assay Total RNAs were extracted by TRizol (ThermoFisher, USA) from stable shFTO MDA-MB-231 cells and the controls. .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000.

    Isolation:

    Article Title: Transgenerational Effects of Extended Dauer Diapause on Starvation Survival and Gene Expression Plasticity in Caenorhabditis elegans
    Article Snippet: .. RNA was isolated using TRIzol (Invitrogen) using the manufacturer’s protocol with minor modifications; 1 ml of TRIzol was used per sample along with 100 μl of acid-washed sand. mRNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) in two batches, utilizing either 500 or 100 ng of starting RNA per library and 12 or 15 PCR cycles, respectively. .. Libraries were sequenced using Illumina HiSeq 4000 to obtain single-end 50 bp reads.

    Article Title: Rare cell detection by single cell RNA sequencing as guided by single molecule RNA FISH
    Article Snippet: .. We sequenced mRNA in bulk from WM989-A6 populations as per Shaffer et. al. We isolated mRNA and built sequencing libraries using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina. ..

    Article Title: Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting
    Article Snippet: .. For RNA-seq of C1R-CD1d parental cells, the same procedures were followed, except for the fact that libraries were prepared .using the NEBNext poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra RNA Library Prep kit for Illumina (New Engand Biolabs) and sequenced on an Illumina HiSeq 2500 platform to obtain paired-end 100bp reads. .. Reads obtained from parental C1R-CD1d RNA-seq experiments were aligned using TopHat (v2.0.14) ( ) against the Hg19 human reference genome using the default settings.

    Article Title: Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation
    Article Snippet: .. Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacturer protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490). .. The RNAseq data was generated on NextSeq500 mid-output (75 bp paired-end reads) kit v2 (Illumina, FC-404-2005).

    Article Title: Rare cell variability and drug-induced reprogramming as a mode of cancer drug resistance
    Article Snippet: .. We used the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina to extract polyadenylated RNA and prepare barcoded RNA sequencing libraries. ..

    Multiplex Assay:

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: .. Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions. .. Then, the final purified libraries were evaluated using a BioAnalyzer 2100 and quantified.

    Purification:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000. ..

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions. .. Then, the final purified libraries were evaluated using a BioAnalyzer 2100 and quantified.

    Sequencing:

    Article Title: Rare cell detection by single cell RNA sequencing as guided by single molecule RNA FISH
    Article Snippet: .. We sequenced mRNA in bulk from WM989-A6 populations as per Shaffer et. al. We isolated mRNA and built sequencing libraries using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina. ..

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: Paragraph title: Illumina sequencing sample preparation for comprehensive RNAseq ... The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S).

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000. ..

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: .. Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions. .. Then, the final purified libraries were evaluated using a BioAnalyzer 2100 and quantified.

    Article Title: Transcriptomic Analysis of Differentially Expressed Genes during Flower Organ Development in Genetic Male Sterile and Male Fertile Tagetes erecta by Digital Gene-Expression Profiling
    Article Snippet: .. Sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s protocols and index codes were added to attribute sequences to each sample. ..

    Article Title: Effects of anticholinergic agent on miRNA profiles and transcriptomes in a murine model of allergic rhinitis
    Article Snippet: .. mRNA library preparation for transcriptome sequencing and data analysis The mRNA libraries were generated using NEBNext® Ultra™ RNA Library Prep kit for Illumina® (New England BioLabs, Inc., Ipswich, MA, USA) according to the manufacturer's instructions. ..

    Quantitative RT-PCR:

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: Enrichment of m6A containing mRNA was analyzed either by qRT-PCR with the primers listed in Additional file : Table S1 or by high-throughput RNA sequencing. .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000.

    cDNA Library Assay:

    Article Title: The primary transcriptome, small RNAs and regulation of antimicrobial resistance in Acinetobacter baumannii ATCC 17978
    Article Snippet: For dRNA-seq, one of the two aliquots were digested with Terminator Exonuclease (TEX) prior to cDNA library preparation ( , ). .. Libraries of the ESP RNA samples were prepared using the NEBNext™ Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions.

    Software:

    Article Title: Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting
    Article Snippet: Base calling and demultiplexing and conversion to fastq file were performed using the ‘Real Time Analysis’ (RTA) and bcl2fastq2 v2.18 illumina software respectively. .. For RNA-seq of C1R-CD1d parental cells, the same procedures were followed, except for the fact that libraries were prepared .using the NEBNext poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra RNA Library Prep kit for Illumina (New Engand Biolabs) and sequenced on an Illumina HiSeq 2500 platform to obtain paired-end 100bp reads.

    RNA Extraction:

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: Paragraph title: RNA extraction and sequencing ... Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions.

    Article Title: Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation
    Article Snippet: Paragraph title: RNA extraction, library construction and data generation and analysis ... Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacturer protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490).

    Sample Prep:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: Paragraph title: Illumina sequencing sample preparation for comprehensive RNAseq ... The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S).

    Next-Generation Sequencing:

    Article Title: The primary transcriptome, small RNAs and regulation of antimicrobial resistance in Acinetobacter baumannii ATCC 17978
    Article Snippet: Paragraph title: Library preparation for next generation sequencing ... Libraries of the ESP RNA samples were prepared using the NEBNext™ Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions.

    Produced:

    Article Title: Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation
    Article Snippet: .. Six RNAseq libraries (NEBNext® Ultra™ RNA Library Prep Kit for Illumina, cat no. E7530) were produced according to manufacturer protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490). .. The RNAseq data was generated on NextSeq500 mid-output (75 bp paired-end reads) kit v2 (Illumina, FC-404-2005).

    Immunoprecipitation:

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: Paragraph title: RNA m6A sequence and m6A-RNA immunoprecipitation assay ... For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000.

    Marker:

    Article Title: Rare cell variability and drug-induced reprogramming as a mode of cancer drug resistance
    Article Snippet: We used the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina to extract polyadenylated RNA and prepare barcoded RNA sequencing libraries. .. We then used R to perform differential expression analysis with DESeq2 (code available at https://bitbucket.org/arjunrajlaboratory/rajlabseqtools ) to identify resistance marker genes.

    FACS:

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000. .. Sequencing reads were aligned to the human genome GRCh37/hg19 by Bowtie2, and the m6A peaks were detected by magnetic cell sorting as described [ ].

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    New England Biolabs ultra ii directional rna library prep kit
    Loss of Pnt results in Naa-to-Nab transformations in diverse sensillar subtypes. a A sensillum can contain up to four OSNs through differentiation of Naa (cyan), Nab (magenta), Nba (green), Nbb (yellow) terminal daughter cells originating from a single SOP lineage. b Representative images of <t>RNA</t> FISH for Or67d (magenta) in at1 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or67d-expressing OSNs are duplicated (arrow). A schematic of the proposed Naa-to-Nab fate transformation is shown on the right (color scheme as in ( a )). Scale bar = 2 µm. The open circles in this and other schematics represent OSN precursors that have undergone apoptosis. c Representative images of RNA FISH for Or85a (magenta) and Or59b (green) in ab2 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or85a OSNs (Nab) are duplicated (arrow), while Or59b OSNs (Nba) are unaffected. d Representative images of RNA FISH for Or85b (magenta) and Or22a (green) in ab3 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or85b OSNs (Nab) are duplicated (arrow), while Or22a OSNs (Nba) are unaffected. e Representative images of RNA FISH for Or92a (magenta) and Or10a (cyan) in ab1 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or92a OSNs (Nab) are duplicated (arrow), while Or10a OSNs (Naa) are lost. f Top: theoretical ratios of OSN types in 2-, 3- and 4-neuron sensilla in control and pnt RNAi antennae, assuming Naa-to-Nab fate transformation (i.e. loss of Naa OSNs, and duplication of Nab OSNs). Bottom: experimentally determined OSN ratios in all sensilla in pnt RNAi antennae using as a proxy the normalized ratios of olfactory receptor <t>mRNA</t> expression from antennal transcriptomes (see Supplementary Fig. 6e ). In ab10, Or49a is reported to be coexpressed with Or85f 13 , but transcript levels for this gene were below the cut-off applied during the analysis of these RNA-seq datasets
    Ultra Ii Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra ii directional rna library prep kit/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    ultra ii directional rna library prep kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs ultra directional rna library prep kit
    Internode zones have unique <t>RNAseq</t> profiles and contain stem- and zone-specific genes. a Principal component analysis of FPKM values obtained from <t>RNA</t> sequencing of the MsZ, CEZ, TZ, and MZ of internode 5. b Venn diagram of genes up-regulated greater than a log 2 fold change of one against the background levels in the whole plant (WP) transcriptome. c Table showing the number of genes expressed > 1 FPKM, number of genes up-regulated against the WP transcriptome with > 1 log 2 fold, number of identified ‘stem-specific’ genes with > 2.5 log 2 fold change against the WP background and > 80 FPKM in any internode zone, and the number of ‘zone-specific’ genes with, in addition to being ‘stem-specific’, > 2 log 2 fold change against all other internodal zones (with some exceptions, see Additional file 2 )
    Ultra Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra directional rna library prep kit/product/New England Biolabs
    Average 99 stars, based on 558 article reviews
    Price from $9.99 to $1999.99
    ultra directional rna library prep kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Loss of Pnt results in Naa-to-Nab transformations in diverse sensillar subtypes. a A sensillum can contain up to four OSNs through differentiation of Naa (cyan), Nab (magenta), Nba (green), Nbb (yellow) terminal daughter cells originating from a single SOP lineage. b Representative images of RNA FISH for Or67d (magenta) in at1 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or67d-expressing OSNs are duplicated (arrow). A schematic of the proposed Naa-to-Nab fate transformation is shown on the right (color scheme as in ( a )). Scale bar = 2 µm. The open circles in this and other schematics represent OSN precursors that have undergone apoptosis. c Representative images of RNA FISH for Or85a (magenta) and Or59b (green) in ab2 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or85a OSNs (Nab) are duplicated (arrow), while Or59b OSNs (Nba) are unaffected. d Representative images of RNA FISH for Or85b (magenta) and Or22a (green) in ab3 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or85b OSNs (Nab) are duplicated (arrow), while Or22a OSNs (Nba) are unaffected. e Representative images of RNA FISH for Or92a (magenta) and Or10a (cyan) in ab1 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or92a OSNs (Nab) are duplicated (arrow), while Or10a OSNs (Naa) are lost. f Top: theoretical ratios of OSN types in 2-, 3- and 4-neuron sensilla in control and pnt RNAi antennae, assuming Naa-to-Nab fate transformation (i.e. loss of Naa OSNs, and duplication of Nab OSNs). Bottom: experimentally determined OSN ratios in all sensilla in pnt RNAi antennae using as a proxy the normalized ratios of olfactory receptor mRNA expression from antennal transcriptomes (see Supplementary Fig. 6e ). In ab10, Or49a is reported to be coexpressed with Or85f 13 , but transcript levels for this gene were below the cut-off applied during the analysis of these RNA-seq datasets

    Journal: Nature Communications

    Article Title: Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system

    doi: 10.1038/s41467-019-08345-4

    Figure Lengend Snippet: Loss of Pnt results in Naa-to-Nab transformations in diverse sensillar subtypes. a A sensillum can contain up to four OSNs through differentiation of Naa (cyan), Nab (magenta), Nba (green), Nbb (yellow) terminal daughter cells originating from a single SOP lineage. b Representative images of RNA FISH for Or67d (magenta) in at1 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or67d-expressing OSNs are duplicated (arrow). A schematic of the proposed Naa-to-Nab fate transformation is shown on the right (color scheme as in ( a )). Scale bar = 2 µm. The open circles in this and other schematics represent OSN precursors that have undergone apoptosis. c Representative images of RNA FISH for Or85a (magenta) and Or59b (green) in ab2 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or85a OSNs (Nab) are duplicated (arrow), while Or59b OSNs (Nba) are unaffected. d Representative images of RNA FISH for Or85b (magenta) and Or22a (green) in ab3 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or85b OSNs (Nab) are duplicated (arrow), while Or22a OSNs (Nba) are unaffected. e Representative images of RNA FISH for Or92a (magenta) and Or10a (cyan) in ab1 sensilla in control and pnt RNAi antennae. In pnt RNAi antennae, Or92a OSNs (Nab) are duplicated (arrow), while Or10a OSNs (Naa) are lost. f Top: theoretical ratios of OSN types in 2-, 3- and 4-neuron sensilla in control and pnt RNAi antennae, assuming Naa-to-Nab fate transformation (i.e. loss of Naa OSNs, and duplication of Nab OSNs). Bottom: experimentally determined OSN ratios in all sensilla in pnt RNAi antennae using as a proxy the normalized ratios of olfactory receptor mRNA expression from antennal transcriptomes (see Supplementary Fig. 6e ). In ab10, Or49a is reported to be coexpressed with Or85f 13 , but transcript levels for this gene were below the cut-off applied during the analysis of these RNA-seq datasets

    Article Snippet: RNA-seq libraries were prepared from the mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).

    Techniques: Fluorescence In Situ Hybridization, Expressing, Transformation Assay, RNA Sequencing Assay

    An OSN lineage-specific driver. a Top row: developmental expression of the nonimmortalized GMR82D08-GAL4 (hereafter, at1 driver) using a myr:GFP reporter (green) in the antennal disc SOPs (region marked by α-Dac (blue)) during late larval/early pupal stages. Bottom row: the at1 driver is expressed in the daughter cells of these SOPs in the developing pupal antenna but progressively loses its expression from 20 h APF as OSNs differentiate (visualized with the neuronal marker α-Elav (magenta)). Scale bar = 20 µm in this and other panels. b Immortalization of the at1 driver reveals labeling of clusters of cells in the adult antenna by an rCD2:GFP reporter (green). RNA fluorescence in situ hybridization demonstrates that a single cell within each cluster (arrowheads in the inset images) expresses Or67d mRNA (magenta). c Representative example of a single sensillum in the adult antenna labeled by the immortalized at1 driver, viewed at three focal planes. There is a single Or67d mRNA-positive OSN (cell 1, arrowhead), flanked by four non-neuronal support cells (cells 2–5). d Sensilla cells labeled by the immortalized at1 driver lineage (α-GFP; green) also express Lush (magenta), an odorant binding protein unique to trichoid sensilla support cells 72

    Journal: Nature Communications

    Article Title: Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system

    doi: 10.1038/s41467-019-08345-4

    Figure Lengend Snippet: An OSN lineage-specific driver. a Top row: developmental expression of the nonimmortalized GMR82D08-GAL4 (hereafter, at1 driver) using a myr:GFP reporter (green) in the antennal disc SOPs (region marked by α-Dac (blue)) during late larval/early pupal stages. Bottom row: the at1 driver is expressed in the daughter cells of these SOPs in the developing pupal antenna but progressively loses its expression from 20 h APF as OSNs differentiate (visualized with the neuronal marker α-Elav (magenta)). Scale bar = 20 µm in this and other panels. b Immortalization of the at1 driver reveals labeling of clusters of cells in the adult antenna by an rCD2:GFP reporter (green). RNA fluorescence in situ hybridization demonstrates that a single cell within each cluster (arrowheads in the inset images) expresses Or67d mRNA (magenta). c Representative example of a single sensillum in the adult antenna labeled by the immortalized at1 driver, viewed at three focal planes. There is a single Or67d mRNA-positive OSN (cell 1, arrowhead), flanked by four non-neuronal support cells (cells 2–5). d Sensilla cells labeled by the immortalized at1 driver lineage (α-GFP; green) also express Lush (magenta), an odorant binding protein unique to trichoid sensilla support cells 72

    Article Snippet: RNA-seq libraries were prepared from the mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).

    Techniques: Expressing, Marker, Labeling, Fluorescence, In Situ Hybridization, Binding Assay

    An OSN lineage-specific driver. a Top row: developmental expression of the nonimmortalized GMR82D08-GAL4 (hereafter, at1 driver) using a myr:GFP reporter (green) in the antennal disc SOPs (region marked by α-Dac (blue)) during late larval/early pupal stages. Bottom row: the at1 driver is expressed in the daughter cells of these SOPs in the developing pupal antenna but progressively loses its expression from 20 h APF as OSNs differentiate (visualized with the neuronal marker α-Elav (magenta)). Scale bar = 20 µm in this and other panels. b Immortalization of the at1 driver reveals labeling of clusters of cells in the adult antenna by an rCD2:GFP reporter (green). RNA fluorescence in situ hybridization demonstrates that a single cell within each cluster (arrowheads in the inset images) expresses Or67d mRNA (magenta). c Representative example of a single sensillum in the adult antenna labeled by the immortalized at1 driver, viewed at three focal planes. There is a single Or67d mRNA-positive OSN (cell 1, arrowhead), flanked by four non-neuronal support cells (cells 2–5). d

    Journal: Nature Communications

    Article Title: Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system

    doi: 10.1038/s41467-019-08345-4

    Figure Lengend Snippet: An OSN lineage-specific driver. a Top row: developmental expression of the nonimmortalized GMR82D08-GAL4 (hereafter, at1 driver) using a myr:GFP reporter (green) in the antennal disc SOPs (region marked by α-Dac (blue)) during late larval/early pupal stages. Bottom row: the at1 driver is expressed in the daughter cells of these SOPs in the developing pupal antenna but progressively loses its expression from 20 h APF as OSNs differentiate (visualized with the neuronal marker α-Elav (magenta)). Scale bar = 20 µm in this and other panels. b Immortalization of the at1 driver reveals labeling of clusters of cells in the adult antenna by an rCD2:GFP reporter (green). RNA fluorescence in situ hybridization demonstrates that a single cell within each cluster (arrowheads in the inset images) expresses Or67d mRNA (magenta). c Representative example of a single sensillum in the adult antenna labeled by the immortalized at1 driver, viewed at three focal planes. There is a single Or67d mRNA-positive OSN (cell 1, arrowhead), flanked by four non-neuronal support cells (cells 2–5). d

    Article Snippet: RNA-seq libraries were prepared from the mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).

    Techniques: Expressing, Marker, Labeling, Fluorescence, In Situ Hybridization

    Internode zones have unique RNAseq profiles and contain stem- and zone-specific genes. a Principal component analysis of FPKM values obtained from RNA sequencing of the MsZ, CEZ, TZ, and MZ of internode 5. b Venn diagram of genes up-regulated greater than a log 2 fold change of one against the background levels in the whole plant (WP) transcriptome. c Table showing the number of genes expressed > 1 FPKM, number of genes up-regulated against the WP transcriptome with > 1 log 2 fold, number of identified ‘stem-specific’ genes with > 2.5 log 2 fold change against the WP background and > 80 FPKM in any internode zone, and the number of ‘zone-specific’ genes with, in addition to being ‘stem-specific’, > 2 log 2 fold change against all other internodal zones (with some exceptions, see Additional file 2 )

    Journal: Biotechnology for Biofuels

    Article Title: A developing Setaria viridis internode: an experimental system for the study of biomass generation in a C4 model species

    doi: 10.1186/s13068-016-0457-6

    Figure Lengend Snippet: Internode zones have unique RNAseq profiles and contain stem- and zone-specific genes. a Principal component analysis of FPKM values obtained from RNA sequencing of the MsZ, CEZ, TZ, and MZ of internode 5. b Venn diagram of genes up-regulated greater than a log 2 fold change of one against the background levels in the whole plant (WP) transcriptome. c Table showing the number of genes expressed > 1 FPKM, number of genes up-regulated against the WP transcriptome with > 1 log 2 fold, number of identified ‘stem-specific’ genes with > 2.5 log 2 fold change against the WP background and > 80 FPKM in any internode zone, and the number of ‘zone-specific’ genes with, in addition to being ‘stem-specific’, > 2 log 2 fold change against all other internodal zones (with some exceptions, see Additional file 2 )

    Article Snippet: RNAseq libraries were generated with polyA enrichment and rRNA depletion using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, Ipswich, USA, http://www.neb.com ) for Illumina® and were sequenced on an Illumina® HiSeq 2500 at the Max Planck Genome Centre, Cologne, Germany.

    Techniques: RNA Sequencing Assay

    Presence of stable miRNAs in Drosophila melanogaster hemolymph. ( A , B ) Clear HL droplets extruded from fly head and thorax. ( C – F ) Real-time qPCR amplification of selected HL miRNAs and mRNAs. Total RNA including small RNA was extracted from HL samples and analyzed with qPCR to measure the levels of miRNAs and mRNAs. The y -axis represents the relative fluorescence units (RFU) in a semi-log scale. The x -axis represents the cycle at which fluorescence was detected above an automatically determined threshold. ( C ) Amplification plots for miR-14, miR-8, and miR-184 measured in a representative HL sample. ( D ) Amplification plots for miR-14, let-7, bantam, and spiked-in synthetic C. elegans cel-miR-39 RNA in representative HL sample. ( E ) The amplification plots for tubulin, actin, and gapdh mRNAs determined by qPCR using total RNA from HL and S2 cells. Sample from S2 cells are used as a positive control for detecting Drosophila mRNAs by qPCR. The amplification curves of all three mRNAs are superimposed on one another, reflecting the presence of similar amounts of these mRNA in S2 cells. Amplification curves from HL samples show that fluorescent products appear after about 30 cycles, reflecting the significantly lower abundance of these mRNAs in HL relative to S2 cells. ( F ) The cycle threshold (Ct) fold-change of selected miRNA amplified in the absence or presence of RNase A and DNase I. Total RNA was extracted from HL samples spiked with 10 fmoles of cel-miR-39 RNA. The x -axis represents the ratio of raw Ct values from control samples divided by raw Ct values from samples incubated with RNase A and DNase I. The significantly higher magnitude of the Ct fold change of the spiked-in synthetic miRNA relative to those of the miRNA indicates that the HL-miRNA are present in nuclease-resistant, stable form.

    Journal: Genomics Insights

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner

    doi: 10.4137/GEI.S38147

    Figure Lengend Snippet: Presence of stable miRNAs in Drosophila melanogaster hemolymph. ( A , B ) Clear HL droplets extruded from fly head and thorax. ( C – F ) Real-time qPCR amplification of selected HL miRNAs and mRNAs. Total RNA including small RNA was extracted from HL samples and analyzed with qPCR to measure the levels of miRNAs and mRNAs. The y -axis represents the relative fluorescence units (RFU) in a semi-log scale. The x -axis represents the cycle at which fluorescence was detected above an automatically determined threshold. ( C ) Amplification plots for miR-14, miR-8, and miR-184 measured in a representative HL sample. ( D ) Amplification plots for miR-14, let-7, bantam, and spiked-in synthetic C. elegans cel-miR-39 RNA in representative HL sample. ( E ) The amplification plots for tubulin, actin, and gapdh mRNAs determined by qPCR using total RNA from HL and S2 cells. Sample from S2 cells are used as a positive control for detecting Drosophila mRNAs by qPCR. The amplification curves of all three mRNAs are superimposed on one another, reflecting the presence of similar amounts of these mRNA in S2 cells. Amplification curves from HL samples show that fluorescent products appear after about 30 cycles, reflecting the significantly lower abundance of these mRNAs in HL relative to S2 cells. ( F ) The cycle threshold (Ct) fold-change of selected miRNA amplified in the absence or presence of RNase A and DNase I. Total RNA was extracted from HL samples spiked with 10 fmoles of cel-miR-39 RNA. The x -axis represents the ratio of raw Ct values from control samples divided by raw Ct values from samples incubated with RNase A and DNase I. The significantly higher magnitude of the Ct fold change of the spiked-in synthetic miRNA relative to those of the miRNA indicates that the HL-miRNA are present in nuclease-resistant, stable form.

    Article Snippet: One-microgram aliquots of total RNA were used to construct mRNA sequencing libraries (mRNA-Seq) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7420S).

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Fluorescence, Positive Control, Incubation

    Outline of the experimental design and workflow for the integrated analysis of the miRNA-Seq and mRNA-Seq data. Genes that are potentially regulated by the HL-miRNAs were determined by integrating miRNA-Seq and mRNA-Seq data using the following three steps: Step 1 (depicted in green): After identifying the HL-miRNAs enriched relative to BT in either young or old, or both young and old ( Tables 1 and 2 ), we retrieved their computationally predicted target genes from the m 3 RNA database ( http://m3rna.cnb.csic.es ). Step 2 (depicted in blue): mRNA-Seq was utilized to identify three groups of genes that were differentially expressed with age, either upregulated, downregulated, or unchanged in BT. Step 3 (depicted in red): The three groups of genes predicted in step1 as targets of HL-miRNAs were intersected with the three groups of genes identified in step 2. In the figure, ( A ) depicts the derivation of genes that are predicted to be targets of HL-miRNAs enriched in young which are also upregulated with age in BT. These genes (Supplementary Table 1 ) are likely upregulated in BT of old flies because their expression is no longer repressed by HL-miRNAs that are enriched only in young flies. ( B ) Depicts the derivation of genes which are the predicted to be targets of HL-miRNAs enriched in old which are also downregulated with age in BT. These genes (Supplementary Table 2 ) are likely downregulated in BT of old flies because their expression is repressed by HL-miRNAs which are enriched only in old flies. ( C ) Depicts the derivation of genes which are predicted to be targets of HL-miRNAs enriched in both young and old which also do not change expression with age in BT. These genes (Supplementary Table 3) do not change expression with age because the concentration of their regulatory miRNAs in HL does not change with age.

    Journal: Genomics Insights

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner

    doi: 10.4137/GEI.S38147

    Figure Lengend Snippet: Outline of the experimental design and workflow for the integrated analysis of the miRNA-Seq and mRNA-Seq data. Genes that are potentially regulated by the HL-miRNAs were determined by integrating miRNA-Seq and mRNA-Seq data using the following three steps: Step 1 (depicted in green): After identifying the HL-miRNAs enriched relative to BT in either young or old, or both young and old ( Tables 1 and 2 ), we retrieved their computationally predicted target genes from the m 3 RNA database ( http://m3rna.cnb.csic.es ). Step 2 (depicted in blue): mRNA-Seq was utilized to identify three groups of genes that were differentially expressed with age, either upregulated, downregulated, or unchanged in BT. Step 3 (depicted in red): The three groups of genes predicted in step1 as targets of HL-miRNAs were intersected with the three groups of genes identified in step 2. In the figure, ( A ) depicts the derivation of genes that are predicted to be targets of HL-miRNAs enriched in young which are also upregulated with age in BT. These genes (Supplementary Table 1 ) are likely upregulated in BT of old flies because their expression is no longer repressed by HL-miRNAs that are enriched only in young flies. ( B ) Depicts the derivation of genes which are the predicted to be targets of HL-miRNAs enriched in old which are also downregulated with age in BT. These genes (Supplementary Table 2 ) are likely downregulated in BT of old flies because their expression is repressed by HL-miRNAs which are enriched only in old flies. ( C ) Depicts the derivation of genes which are predicted to be targets of HL-miRNAs enriched in both young and old which also do not change expression with age in BT. These genes (Supplementary Table 3) do not change expression with age because the concentration of their regulatory miRNAs in HL does not change with age.

    Article Snippet: One-microgram aliquots of total RNA were used to construct mRNA sequencing libraries (mRNA-Seq) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7420S).

    Techniques: Expressing, Concentration Assay