ultra directional mrnaseq kit  (New England Biolabs)


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  • 99
    Name:
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina
    Description:
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina 96 rxns
    Catalog Number:
    e7420l
    Price:
    3877
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
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    Structured Review

    New England Biolabs ultra directional mrnaseq kit
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina
    NEBNext Ultra Directional RNA Library Prep Kit for Illumina 96 rxns
    https://www.bioz.com/result/ultra directional mrnaseq kit/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultra directional mrnaseq kit - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells"

    Article Title: Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells

    Journal: eLife

    doi: 10.7554/eLife.34081

    Dynamics of H3K4me3 and expression in subsets of genes. ( A ) Genomic regions subject to age-linked CNV detected by comparison between input samples; these were excluded from ChIP analysis. ( B ) Scatter plot of log 2 -transformed mRNAseq read counts from protein coding genes defined in Figure 4A normalised for ORF length comparing log phase cells to 48-hr-aged cells. Data averaged across five replicates per condition. ( C ) Average profile of H3K4me3 ChIP signal for 1 kb either side of the TSS performed as in Figure 4C , for 300 genes least induced with age. ( D ) As ( C ) for 300 most induced genes. ( E ) Hierarchical clustering analysis of log 2 -transformed mRNA levels across age for the 4806 unambiguous genes defined in Figure 4A .
    Figure Legend Snippet: Dynamics of H3K4me3 and expression in subsets of genes. ( A ) Genomic regions subject to age-linked CNV detected by comparison between input samples; these were excluded from ChIP analysis. ( B ) Scatter plot of log 2 -transformed mRNAseq read counts from protein coding genes defined in Figure 4A normalised for ORF length comparing log phase cells to 48-hr-aged cells. Data averaged across five replicates per condition. ( C ) Average profile of H3K4me3 ChIP signal for 1 kb either side of the TSS performed as in Figure 4C , for 300 genes least induced with age. ( D ) As ( C ) for 300 most induced genes. ( E ) Hierarchical clustering analysis of log 2 -transformed mRNA levels across age for the 4806 unambiguous genes defined in Figure 4A .

    Techniques Used: Expressing, Chromatin Immunoprecipitation, Transformation Assay

    Related Articles

    Multiplex Assay:

    Article Title: Transcriptomic dataset reveals the molecular basis of genotypic variation in hexaploid wheat (T. aestivum L.) in response to Fe/Zn deficiency
    Article Snippet: .. Purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext® Ultra™ Directional RNA Library Prep Kit protocol. ..

    Article Title: Whole genome screen reveals a novel relationship between Wolbachia levels and Drosophila host translation
    Article Snippet: .. After rRNA depletion libraries were prepared according to manufacturer’s instructions using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, E7420L) and NEBNext Multiplex Oligos for Illumina Index Primers Set I (Illumina, E7335). .. After adaptor ligation, the libraries were amplified by qPCR using the KAPA Real-time amplification kit (KAPA Biosystems).

    RNA Sequencing Assay:

    Article Title: Heterochromatin-associated interactions of Drosophila HP1a with dADD1, HIPP1, and repetitive RNAs
    Article Snippet: .. An NEBNext ultradirectional RNA library kit (New England Biolabs, catalog no. E7420S) was used to make cDNA and RNA-seq libraries. .. Several minor modifications to the standard kit protocol were made based on (1) size (RNA fragmentation time was decreased from 15 to 5 min) and (2) the small amount of RNA (the number of PCR cycles were adjusted to 20 cycles in the USER excision and PCR library step).

    Construct:

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner
    Article Snippet: .. One-microgram aliquots of total RNA were used to construct mRNA sequencing libraries (mRNA-Seq) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7420S). .. Briefly, poly(A)+ RNA was purified with oligo-d(T) beads, and fragmented to ~200 nucleotide lengths prior to cDNA synthesis using random primers.

    Purification:

    Article Title: Transcriptomic dataset reveals the molecular basis of genotypic variation in hexaploid wheat (T. aestivum L.) in response to Fe/Zn deficiency
    Article Snippet: .. Purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext® Ultra™ Directional RNA Library Prep Kit protocol. ..

    Generated:

    Article Title: A developing Setaria viridis internode: an experimental system for the study of biomass generation in a C4 model species
    Article Snippet: .. RNAseq libraries were generated with polyA enrichment and rRNA depletion using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, Ipswich, USA, http://www.neb.com ) for Illumina® and were sequenced on an Illumina® HiSeq 2500 at the Max Planck Genome Centre, Cologne, Germany. .. RNAseq analysis Illumina 100 bp paired-end reads from four biological replicates of the four internode zones were imported into CLC genomics workbench 8 (CLCbio, Aarhus, Denmark, http://www.clcbio.com ) for adapter removal, trimming, mapping, and read counting.

    other:

    Article Title: Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification
    Article Snippet: Input sample libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on an Illumina HiSeq2000 at the Tayside Centre for Genomics Analysis, University of Dundee, with a pair-end read length of 75 bp.

    Sequencing:

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner
    Article Snippet: .. One-microgram aliquots of total RNA were used to construct mRNA sequencing libraries (mRNA-Seq) using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7420S). .. Briefly, poly(A)+ RNA was purified with oligo-d(T) beads, and fragmented to ~200 nucleotide lengths prior to cDNA synthesis using random primers.

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  • 99
    New England Biolabs ultra directional mrnaseq kit
    Dynamics of H3K4me3 and expression in subsets of genes. ( A ) Genomic regions subject to age-linked CNV detected by comparison between input samples; these were excluded from ChIP analysis. ( B ) Scatter plot of log 2 -transformed <t>mRNAseq</t> read counts from protein coding genes defined in Figure 4A normalised for ORF length comparing log phase cells to 48-hr-aged cells. Data averaged across five replicates per condition. ( C ) Average profile of H3K4me3 ChIP signal for 1 kb either side of the TSS performed as in Figure 4C , for 300 genes least induced with age. ( D ) As ( C ) for 300 most induced genes. ( E ) Hierarchical clustering analysis of log 2 -transformed mRNA levels across age for the 4806 unambiguous genes defined in Figure 4A .
    Ultra Directional Mrnaseq Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra directional mrnaseq kit/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ultra directional mrnaseq kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Dynamics of H3K4me3 and expression in subsets of genes. ( A ) Genomic regions subject to age-linked CNV detected by comparison between input samples; these were excluded from ChIP analysis. ( B ) Scatter plot of log 2 -transformed mRNAseq read counts from protein coding genes defined in Figure 4A normalised for ORF length comparing log phase cells to 48-hr-aged cells. Data averaged across five replicates per condition. ( C ) Average profile of H3K4me3 ChIP signal for 1 kb either side of the TSS performed as in Figure 4C , for 300 genes least induced with age. ( D ) As ( C ) for 300 most induced genes. ( E ) Hierarchical clustering analysis of log 2 -transformed mRNA levels across age for the 4806 unambiguous genes defined in Figure 4A .

    Journal: eLife

    Article Title: Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells

    doi: 10.7554/eLife.34081

    Figure Lengend Snippet: Dynamics of H3K4me3 and expression in subsets of genes. ( A ) Genomic regions subject to age-linked CNV detected by comparison between input samples; these were excluded from ChIP analysis. ( B ) Scatter plot of log 2 -transformed mRNAseq read counts from protein coding genes defined in Figure 4A normalised for ORF length comparing log phase cells to 48-hr-aged cells. Data averaged across five replicates per condition. ( C ) Average profile of H3K4me3 ChIP signal for 1 kb either side of the TSS performed as in Figure 4C , for 300 genes least induced with age. ( D ) As ( C ) for 300 most induced genes. ( E ) Hierarchical clustering analysis of log 2 -transformed mRNA levels across age for the 4806 unambiguous genes defined in Figure 4A .

    Article Snippet: 500 ng RNA was used to prepare libraries using the NEBNext Ultra Directional mRNAseq kit (NEB E7420, E7490, E7335, E7500, E7710) as described with modifications: Reagent volumes for RT, second strand synthesis, tailing and ligation were reduced by 50%, while libraries were amplified for 12 cycles using 2 µl each primer per reaction before two rounds of AMPure bead purification as described in the kit at 0.9x ratio prior to quality control using a Bioanalyzer and quantification usinng KAPA Biosystems kit KK4824.

    Techniques: Expressing, Chromatin Immunoprecipitation, Transformation Assay