fluorophore conjugated uea 1 lectin (Vector Laboratories)
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Vector Laboratories
fluorophore conjugated uea 1 lectin
Fluorophore Conjugated Uea 1 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 522 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uea+1/Biotinylated+Ulex+Europaeus+Agglutinin+I+(UEA+I)/10__1038_slash_s41467___026___78216___2-653-11-14
Average 96 stars, based on 522 article reviews
Fluorophore Conjugated Uea 1 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 522 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uea+1/Biotinylated+Ulex+Europaeus+Agglutinin+I+(UEA+I)/10__1038_slash_s41467___026___78216___2-653-11-14
Average 96 stars, based on 522 article reviews
fluorophore conjugated uea 1 lectin - by Bioz Stars,
2026-10
96/100 stars
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Staining:Article Title: Defining enteric bacterial pathogenesis using organoids: Citrobacter rodentium uses EspC, an atypical mucinolytic protease, to penetrate mouse colonic mucus. Article Snippet: .. The sections were stained with the following primary antibodies and lectins: rabbit anti-LPS (Bio-Rad, OBT0986) for C. rodentium, rabbit anti-Muc2 (Boster, A01212; Novus Biologicals, NBP1–31231), Article Title: UBL3 Participates in the Early Stages of CD83‐Dependent CD4 + T Cell Selection Article Snippet: Absolute cell numbers were obtained using an internal microsphere counting standard (BD Biosciences). .. Cell suspensions were stained with antibodies specific for MHC II (M5/114.15.2, WEHI Antibody Facility, PE), CD45 (30‐F11, PerCP), CD326 (G8.8, APC‐Cy7), Ly51 (6C3, FITC) (all Biolegend), and Article Title: UBL3 Participates in the Early Stages of CD83‐Dependent CD4 + T Cell Selection Article Snippet: Cells were run through magnetic LS columns to remove CD45 + cells (Miltenyi Biotec). .. Flowthrough was collected and stained with antibodies specific for CD45 (30‐F11, PE), CD326 (G8.8, APC‐Cy7), Ly51 (6C3, FITC) (all Biolegend) and Article Title: Defining enteric bacterial pathogenesis using organoids: Citrobacter rodentium uses EspC, an atypical mucinolytic protease, to penetrate mouse colonic mucus Article Snippet: .. The sections were stained with the following primary antibodies and lectins: rabbit anti-LPS (Bio-Rad, OBT0986) for C. rodentium , rabbit anti-Muc2 (Boster, A01212; Novus Biologicals, NBP1–31231), Immunopeptidomics:Article Title: UBL3 Participates in the Early Stages of CD83‐Dependent CD4 + T Cell Selection Article Snippet: Absolute cell numbers were obtained using an internal microsphere counting standard (BD Biosciences). .. Cell suspensions were stained with antibodies specific for MHC II (M5/114.15.2, WEHI Antibody Facility, PE), CD45 (30‐F11, PerCP), CD326 (G8.8, APC‐Cy7), Ly51 (6C3, FITC) (all Biolegend), and Flow Cytometry:Article Title: UBL3 Participates in the Early Stages of CD83‐Dependent CD4 + T Cell Selection Article Snippet: Absolute cell numbers were obtained using an internal microsphere counting standard (BD Biosciences). .. Cell suspensions were stained with antibodies specific for MHC II (M5/114.15.2, WEHI Antibody Facility, PE), CD45 (30‐F11, PerCP), CD326 (G8.8, APC‐Cy7), Ly51 (6C3, FITC) (all Biolegend), and Whole Genome Amplification:Article Title: Using MUC2 mucin producing tumorigenic human goblet-like cells to uncover functional properties of the mucus barrier. Article Snippet: Briefly, coverslips were washed in PBS, fixed with paraformaldehyde (4%) for 15 min at 37°C, washed with PBS, and permeabilized with 0.35% Triton for 5 min. After three washes with PBST (0.1% Tween-20 in PBS), coverslips were blocked for 1 h with donkey serum (5%), washed, and incubated overnight with antibodies for MUC2 (sc -515,106, Santa Cruz) or FCGBP (PAP389Hu01, Cloud-Clone) at 4°C in a humidified chamber. .. The next day, PBST was used to wash the coverslips prior to incubating with fluorescent secondary antibodies and DAPI (Life Technologies) for 1 h. Lectins were imaged with PNA (FL-1071, Vector Laboratories), DBA (FL-1031, Vector Laboratories), ConA (FL-1001, Vector Laboratories), SNA/ EBL (FL-1301, Vector Laboratories), Article Title: Using MUC2 mucin producing tumorigenic human goblet-like cells to uncover functional properties of the mucus barrier Article Snippet: Briefly, coverslips were washed in PBS, fixed with paraformaldehyde (4%) for 15 min at 37°C, washed with PBS, and permeabilized with 0.35% Triton for 5 min. After three washes with PBST (0.1% Tween-20 in PBS), coverslips were blocked for 1 h with donkey serum (5%), washed, and incubated overnight with antibodies for MUC2 (sc -515,106, Santa Cruz) or FCGBP (PAP389Hu01, Cloud-Clone) at 4°C in a humidified chamber. .. The next day, PBST was used to wash the coverslips prior to incubating with fluorescent secondary antibodies and DAPI (Life Technologies) for 1 h. Lectins were imaged with PNA (FL-1071, Vector Laboratories), DBA (FL-1031, Vector Laboratories), ConA (FL-1001, Vector Laboratories), SNA/EBL (FL-1301, Vector Laboratories), Article Title: In mouse and in vitro models, bowel preparation promotes pathogen colonization, translocation, and exacerbation of inflammation Article Snippet: Slides were then incubated in 99.5% ethanol for 5 min, then left to dry and circled with a PAP (liquid blocker) pen (Fisher Scientific). .. DAPI (10 μg/mL, Sigma Aldrich), Article Title: In mouse and in vitro models, bowel preparation promotes pathogen colonization, translocation, and exacerbation of inflammation. Article Snippet: Paraffin was removed from sectioned slides through incubation at 60 ◦ C for 10 min, followed by 2x 10-min incubations in pre-warmed xylenes at 60 ◦ C. Slides were then incubated in 99.5% ethanol for 5 min, then left to dry and circled with a PAP (liquid blocker) pen (Fisher Scientific). .. 108 DAPI (10 μg/mL, Sigma Aldrich), Incubation:Article Title: In mouse and in vitro models, bowel preparation promotes pathogen colonization, translocation, and exacerbation of inflammation Article Snippet: Slides were then incubated in 99.5% ethanol for 5 min, then left to dry and circled with a PAP (liquid blocker) pen (Fisher Scientific). .. DAPI (10 μg/mL, Sigma Aldrich), Article Title: In mouse and in vitro models, bowel preparation promotes pathogen colonization, translocation, and exacerbation of inflammation. Article Snippet: Paraffin was removed from sectioned slides through incubation at 60 ◦ C for 10 min, followed by 2x 10-min incubations in pre-warmed xylenes at 60 ◦ C. Slides were then incubated in 99.5% ethanol for 5 min, then left to dry and circled with a PAP (liquid blocker) pen (Fisher Scientific). .. 108 DAPI (10 μg/mL, Sigma Aldrich), |
![Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus <t>agglutinin</t> <t>1</t> <t>(UEA1)</t> + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4582/pmc13084582/pmc13084582__gr3.jpg)