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hipsc line ucsd167i 99 1  (WiCell Research Institute Inc)


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    WiCell Research Institute Inc hipsc line ucsd167i 99 1
    Hipsc Line Ucsd167i 99 1, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 5 article reviews
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    93/100 stars

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    Characterization of the <t>UCSD167i-99-1</t> hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.
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    Characterization of the <t>UCSD167i-99-1</t> hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.
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    Characterization of the <t>UCSD167i-99-1</t> hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.
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    Characterization of <t>the</t> <t>UCSD167i-99-1</t> <t>hiPSC</t> line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.
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    Characterization of the UCSD167i-99-1 hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Optimizing Nodal, Wnt and BMP signaling pathways for robust and efficient differentiation of human induced pluripotent stem cells to intermediate mesoderm cells

    doi: 10.3389/fcell.2024.1395723

    Figure Lengend Snippet: Characterization of the UCSD167i-99-1 hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.

    Article Snippet: For this purpose, we adopted the strategy outlined by and investigated the roles of high levels of Nodal and BMP signaling activity, along with WNT signaling, in the determination of IM cells derived from a UCSD167i-99-1 hiPSC line, obtained from the WiCell Research Institute.

    Techniques: Immunofluorescence, Staining, Marker, Expressing, Quantitative RT-PCR

    Characterization of the UCSD167i-99-1 hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Optimizing Nodal, Wnt and BMP signaling pathways for robust and efficient differentiation of human induced pluripotent stem cells to intermediate mesoderm cells

    doi: 10.3389/fcell.2024.1395723

    Figure Lengend Snippet: Characterization of the UCSD167i-99-1 hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.

    Article Snippet: The hiPSC line used in our study was UCSD167i-99-1 obtained from the WiCell Research Institute (Madison, WI).

    Techniques: Immunofluorescence, Staining, Marker, Expressing, Quantitative RT-PCR

    Characterization of the UCSD167i-99-1 hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Optimizing Nodal, Wnt and BMP signaling pathways for robust and efficient differentiation of human induced pluripotent stem cells to intermediate mesoderm cells

    doi: 10.3389/fcell.2024.1395723

    Figure Lengend Snippet: Characterization of the UCSD167i-99-1 hiPSC line. (A) Schematic diagram of the differentiation strategy adopted by with modifications, to induce hiPSC into MP and IM cells. (B–D) Immunofluorescence images of hiPSC colonies stained for POU5F1 (green), NANOG (green), SOX2 (red), merged with nucleus marker DAPI (cyan). Scale bars: 100 μm. (E) Absolute mRNA expression levels of pluripotency markers determined by RT - qPCR analysis in undifferentiated hiPSC colonies. Error bars represent mean ± SEM, n = 3 independent experiments, n.s. not significant, * p < 0.05 , ** p < 0.01, *** p < 0.001, **** p < 0.0001. hiPSC human induced pluripotent stem cells, MP mesoderm progenitor, IM intermediate mesoderm, DE definitive endoderm.

    Article Snippet: The hiPSC line used in our study was UCSD167i-99-1 obtained from the WiCell Research Institute (Madison, WI).

    Techniques: Immunofluorescence, Staining, Marker, Expressing, Quantitative RT-PCR