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ZIRC Inc uas e1b
Phenotypic characterization of a stable transgenic line. ( a ) Graphic representation of the DNA constructs used for transgenesis and breeding. ( b ) A 24 h.p.f. embryo expresses Smoa1-EGFP in the forebrain and retinal neuroblasts. A 96 h.p.f. double transgenic embryo derived from crossing of the Tg(krt5:Gal4VP16;14 × <t>UAS:smoa1-EGFP)</t> and the <t>Tg(UAS-E1b:nfsB-mCherry)</t> c264 stable lines expressed GFP ( c ) and mCherry ( d ) in the ONs (arrows). ( e ) A 6-month-old wild-type and ( f ) a transgenic fish with a gross tumor in its left eye (arrow). ( g ) Wild-type fish formed a normal optic chiasm, whereas ( h ) transgenic fish failed to form optic chiasm and showed dramatic expansion of the ONH region (arrow). ( i ) Dissected brain and eyes showing an enlarged ON associating with a gross eye tumor (arrow).
Uas E1b, supplied by ZIRC Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uas e1b/product/ZIRC Inc
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
uas e1b - by Bioz Stars, 2021-01
85/100 stars

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1) Product Images from "Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway"

Article Title: Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway

Journal: Oncogenesis

doi: 10.1038/oncsis.2014.10

Phenotypic characterization of a stable transgenic line. ( a ) Graphic representation of the DNA constructs used for transgenesis and breeding. ( b ) A 24 h.p.f. embryo expresses Smoa1-EGFP in the forebrain and retinal neuroblasts. A 96 h.p.f. double transgenic embryo derived from crossing of the Tg(krt5:Gal4VP16;14 × UAS:smoa1-EGFP) and the Tg(UAS-E1b:nfsB-mCherry) c264 stable lines expressed GFP ( c ) and mCherry ( d ) in the ONs (arrows). ( e ) A 6-month-old wild-type and ( f ) a transgenic fish with a gross tumor in its left eye (arrow). ( g ) Wild-type fish formed a normal optic chiasm, whereas ( h ) transgenic fish failed to form optic chiasm and showed dramatic expansion of the ONH region (arrow). ( i ) Dissected brain and eyes showing an enlarged ON associating with a gross eye tumor (arrow).
Figure Legend Snippet: Phenotypic characterization of a stable transgenic line. ( a ) Graphic representation of the DNA constructs used for transgenesis and breeding. ( b ) A 24 h.p.f. embryo expresses Smoa1-EGFP in the forebrain and retinal neuroblasts. A 96 h.p.f. double transgenic embryo derived from crossing of the Tg(krt5:Gal4VP16;14 × UAS:smoa1-EGFP) and the Tg(UAS-E1b:nfsB-mCherry) c264 stable lines expressed GFP ( c ) and mCherry ( d ) in the ONs (arrows). ( e ) A 6-month-old wild-type and ( f ) a transgenic fish with a gross tumor in its left eye (arrow). ( g ) Wild-type fish formed a normal optic chiasm, whereas ( h ) transgenic fish failed to form optic chiasm and showed dramatic expansion of the ONH region (arrow). ( i ) Dissected brain and eyes showing an enlarged ON associating with a gross eye tumor (arrow).

Techniques Used: Transgenic Assay, Construct, Derivative Assay, Fluorescence In Situ Hybridization

2) Product Images from "A zebrafish model of inflammatory lymphangiogenesis"

Article Title: A zebrafish model of inflammatory lymphangiogenesis

Journal: Biology Open

doi: 10.1242/bio.013540

Macrophages are required for intestinal inflammatory lymphangiogenesis. (A) Schema outlining metronidazole and TNBS treatments. (B) Lateral image of the intestine in 7 dpf lyve1:DsRed2; kdrl:EGFP larvae exposed to TNBS+2.5 mM metronidazole (Met). (C,D), Lateral image of the intestine in 7 dpf lyve1:DsRed2;kdrl:EGFP;mpeg1:Gal4FF;UAS:nfsB:mCherry larvae exposed to TNBS (C) and TNBS+2.5 mM metronidazole (D). (E,F), Lateral image of the intestine in 7 dpf lyve1:DsRed2;kdrl:EGFP;mpx:KalTA4;UAS:nfsB:mCherry larvae exposed to TNBS (E) and TNBS+2.5 mM metronidazole (F). (B′-F′) shows expression in the DsRed/mCherry channel only. Asterisks indicate intestinal lymphatic sprouts (ILSs). (G,H) FACS analysis of mCherry-positive cell counts from TNBS-treated (control) and TNBS+2.5 mM metronidazole-treated 3 and 7 dpf mpeg1:Gal4FF;UAS:nfsB:mCherry larvae (G) or mpx:KalTA4;UAS:nfsB:mCherry larvae (H) ( n ≥3, each replicate contains 30–80 larvae). (I,J) Quantification of ILS number (I) and total ILS length (J) ( n ≥17). Error bars, ±s.d. n.s. P > 0.05, * P
Figure Legend Snippet: Macrophages are required for intestinal inflammatory lymphangiogenesis. (A) Schema outlining metronidazole and TNBS treatments. (B) Lateral image of the intestine in 7 dpf lyve1:DsRed2; kdrl:EGFP larvae exposed to TNBS+2.5 mM metronidazole (Met). (C,D), Lateral image of the intestine in 7 dpf lyve1:DsRed2;kdrl:EGFP;mpeg1:Gal4FF;UAS:nfsB:mCherry larvae exposed to TNBS (C) and TNBS+2.5 mM metronidazole (D). (E,F), Lateral image of the intestine in 7 dpf lyve1:DsRed2;kdrl:EGFP;mpx:KalTA4;UAS:nfsB:mCherry larvae exposed to TNBS (E) and TNBS+2.5 mM metronidazole (F). (B′-F′) shows expression in the DsRed/mCherry channel only. Asterisks indicate intestinal lymphatic sprouts (ILSs). (G,H) FACS analysis of mCherry-positive cell counts from TNBS-treated (control) and TNBS+2.5 mM metronidazole-treated 3 and 7 dpf mpeg1:Gal4FF;UAS:nfsB:mCherry larvae (G) or mpx:KalTA4;UAS:nfsB:mCherry larvae (H) ( n ≥3, each replicate contains 30–80 larvae). (I,J) Quantification of ILS number (I) and total ILS length (J) ( n ≥17). Error bars, ±s.d. n.s. P > 0.05, * P

Techniques Used: Expressing, FACS

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Article Title: Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway
Article Snippet: Zebrafish husbandry AB and Tg(UAS-E1b:nfsB-mCherry) c264 strains were acquired from the Zebrafish International Resource Center (ZIRC, Eugene, OR, USA).

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    ZIRC Inc uas e1b
    Phenotypic characterization of a stable transgenic line. ( a ) Graphic representation of the DNA constructs used for transgenesis and breeding. ( b ) A 24 h.p.f. embryo expresses Smoa1-EGFP in the forebrain and retinal neuroblasts. A 96 h.p.f. double transgenic embryo derived from crossing of the Tg(krt5:Gal4VP16;14 × <t>UAS:smoa1-EGFP)</t> and the <t>Tg(UAS-E1b:nfsB-mCherry)</t> c264 stable lines expressed GFP ( c ) and mCherry ( d ) in the ONs (arrows). ( e ) A 6-month-old wild-type and ( f ) a transgenic fish with a gross tumor in its left eye (arrow). ( g ) Wild-type fish formed a normal optic chiasm, whereas ( h ) transgenic fish failed to form optic chiasm and showed dramatic expansion of the ONH region (arrow). ( i ) Dissected brain and eyes showing an enlarged ON associating with a gross eye tumor (arrow).
    Uas E1b, supplied by ZIRC Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uas e1b/product/ZIRC Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    uas e1b - by Bioz Stars, 2021-01
    85/100 stars
      Buy from Supplier

    88
    ZIRC Inc zebrafish husbandry adult zebrafish
    Phenotypic characterization of a stable transgenic line. ( a ) Graphic representation of the DNA constructs used for transgenesis and breeding. ( b ) A 24 h.p.f. embryo expresses Smoa1-EGFP in the forebrain and retinal neuroblasts. A 96 h.p.f. double transgenic embryo derived from crossing of the Tg(krt5:Gal4VP16;14 × <t>UAS:smoa1-EGFP)</t> and the <t>Tg(UAS-E1b:nfsB-mCherry)</t> c264 stable lines expressed GFP ( c ) and mCherry ( d ) in the ONs (arrows). ( e ) A 6-month-old wild-type and ( f ) a transgenic fish with a gross tumor in its left eye (arrow). ( g ) Wild-type fish formed a normal optic chiasm, whereas ( h ) transgenic fish failed to form optic chiasm and showed dramatic expansion of the ONH region (arrow). ( i ) Dissected brain and eyes showing an enlarged ON associating with a gross eye tumor (arrow).
    Zebrafish Husbandry Adult Zebrafish, supplied by ZIRC Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zebrafish husbandry adult zebrafish/product/ZIRC Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zebrafish husbandry adult zebrafish - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

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    Phenotypic characterization of a stable transgenic line. ( a ) Graphic representation of the DNA constructs used for transgenesis and breeding. ( b ) A 24 h.p.f. embryo expresses Smoa1-EGFP in the forebrain and retinal neuroblasts. A 96 h.p.f. double transgenic embryo derived from crossing of the Tg(krt5:Gal4VP16;14 × UAS:smoa1-EGFP) and the Tg(UAS-E1b:nfsB-mCherry) c264 stable lines expressed GFP ( c ) and mCherry ( d ) in the ONs (arrows). ( e ) A 6-month-old wild-type and ( f ) a transgenic fish with a gross tumor in its left eye (arrow). ( g ) Wild-type fish formed a normal optic chiasm, whereas ( h ) transgenic fish failed to form optic chiasm and showed dramatic expansion of the ONH region (arrow). ( i ) Dissected brain and eyes showing an enlarged ON associating with a gross eye tumor (arrow).

    Journal: Oncogenesis

    Article Title: Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway

    doi: 10.1038/oncsis.2014.10

    Figure Lengend Snippet: Phenotypic characterization of a stable transgenic line. ( a ) Graphic representation of the DNA constructs used for transgenesis and breeding. ( b ) A 24 h.p.f. embryo expresses Smoa1-EGFP in the forebrain and retinal neuroblasts. A 96 h.p.f. double transgenic embryo derived from crossing of the Tg(krt5:Gal4VP16;14 × UAS:smoa1-EGFP) and the Tg(UAS-E1b:nfsB-mCherry) c264 stable lines expressed GFP ( c ) and mCherry ( d ) in the ONs (arrows). ( e ) A 6-month-old wild-type and ( f ) a transgenic fish with a gross tumor in its left eye (arrow). ( g ) Wild-type fish formed a normal optic chiasm, whereas ( h ) transgenic fish failed to form optic chiasm and showed dramatic expansion of the ONH region (arrow). ( i ) Dissected brain and eyes showing an enlarged ON associating with a gross eye tumor (arrow).

    Article Snippet: Zebrafish husbandry AB and Tg(UAS-E1b:nfsB-mCherry) c264 strains were acquired from the Zebrafish International Resource Center (ZIRC, Eugene, OR, USA).

    Techniques: Transgenic Assay, Construct, Derivative Assay, Fluorescence In Situ Hybridization

    Macrophages are required for intestinal inflammatory lymphangiogenesis. (A) Schema outlining metronidazole and TNBS treatments. (B) Lateral image of the intestine in 7 dpf lyve1:DsRed2; kdrl:EGFP larvae exposed to TNBS+2.5 mM metronidazole (Met). (C,D), Lateral image of the intestine in 7 dpf lyve1:DsRed2;kdrl:EGFP;mpeg1:Gal4FF;UAS:nfsB:mCherry larvae exposed to TNBS (C) and TNBS+2.5 mM metronidazole (D). (E,F), Lateral image of the intestine in 7 dpf lyve1:DsRed2;kdrl:EGFP;mpx:KalTA4;UAS:nfsB:mCherry larvae exposed to TNBS (E) and TNBS+2.5 mM metronidazole (F). (B′-F′) shows expression in the DsRed/mCherry channel only. Asterisks indicate intestinal lymphatic sprouts (ILSs). (G,H) FACS analysis of mCherry-positive cell counts from TNBS-treated (control) and TNBS+2.5 mM metronidazole-treated 3 and 7 dpf mpeg1:Gal4FF;UAS:nfsB:mCherry larvae (G) or mpx:KalTA4;UAS:nfsB:mCherry larvae (H) ( n ≥3, each replicate contains 30–80 larvae). (I,J) Quantification of ILS number (I) and total ILS length (J) ( n ≥17). Error bars, ±s.d. n.s. P > 0.05, * P

    Journal: Biology Open

    Article Title: A zebrafish model of inflammatory lymphangiogenesis

    doi: 10.1242/bio.013540

    Figure Lengend Snippet: Macrophages are required for intestinal inflammatory lymphangiogenesis. (A) Schema outlining metronidazole and TNBS treatments. (B) Lateral image of the intestine in 7 dpf lyve1:DsRed2; kdrl:EGFP larvae exposed to TNBS+2.5 mM metronidazole (Met). (C,D), Lateral image of the intestine in 7 dpf lyve1:DsRed2;kdrl:EGFP;mpeg1:Gal4FF;UAS:nfsB:mCherry larvae exposed to TNBS (C) and TNBS+2.5 mM metronidazole (D). (E,F), Lateral image of the intestine in 7 dpf lyve1:DsRed2;kdrl:EGFP;mpx:KalTA4;UAS:nfsB:mCherry larvae exposed to TNBS (E) and TNBS+2.5 mM metronidazole (F). (B′-F′) shows expression in the DsRed/mCherry channel only. Asterisks indicate intestinal lymphatic sprouts (ILSs). (G,H) FACS analysis of mCherry-positive cell counts from TNBS-treated (control) and TNBS+2.5 mM metronidazole-treated 3 and 7 dpf mpeg1:Gal4FF;UAS:nfsB:mCherry larvae (G) or mpx:KalTA4;UAS:nfsB:mCherry larvae (H) ( n ≥3, each replicate contains 30–80 larvae). (I,J) Quantification of ILS number (I) and total ILS length (J) ( n ≥17). Error bars, ±s.d. n.s. P > 0.05, * P

    Article Snippet: The Tg(kdrl:EGFP) and Tg(UAS-E1b:nfsB-mCherry) c264 lines were sourced from the Zebrafish international Resource Center (ZIRC, supported by grant P40 RR012546 from the NIH-NCRR).

    Techniques: Expressing, FACS