u87 human epithelial glioblastoma cell line  (ATCC)

Journal: Cells

Article Title: Time- and Dose-Dependent Effects of Ionizing Irradiation on the Membrane Expression of Hsp70 on Glioma Cells

doi: 10.3390/cells9040912

Figure Lengend Snippet: Percentage ( A ) and mean fluorescence intensity (mfi) ( B ) of mHsp70-positive U87 glioblastoma cells after sham irradiation (0 Gy) on days 0, 1, 2, 4, 5, 6 and 7. Bars represent the mean value and the corresponding standard deviation (SD) of n = 3 independent experiments. ANOVA was used to compare data across all days.

Article Snippet: The epithelial human glioblastoma cell line U87 MG (ATCC HTB-14, MGMT - ) and the human hepatocellular carcinoma cell line HepG2 (ATCC HB-8065) were grown in complete growth medium, consisting of Dulbecco’s Eagle’s Minimum Essential Medium (DMEM) (Sigma-Aldrich, Steinheim Germany) supplemented with 10% v / v heat inactivated fetal calf serum (FCS) (Sigma-Aldrich), 1% antibiotics (10,000 IU/mL penicillin, 10 mg/mL streptomycin, Sigma-Aldrich), l -glutamine (Sigma-Aldrich), MEM non-essential amino acid solution 100× (Sigma-Aldrich) and sodium pyruvate (Sigma-Aldrich).The epithelial human cervix carcinoma cell line HeLa (ATCC CCL-2) was grown in complete growth medium, consisting of RPMI-1640 (Sigma-Aldrich, Germany) supplemented with 10% v / v heat inactivated FCS (Sigma-Aldrich), 1% antibiotics (10,000 IU/mL penicillin, 10 mg/mL streptomycin, Sigma-Aldrich), l -glutamine (Sigma-Aldrich) and sodium pyruvate (Sigma-Aldrich).

Techniques: Fluorescence, Irradiation, Standard Deviation

Journal: Cells

Article Title: Time- and Dose-Dependent Effects of Ionizing Irradiation on the Membrane Expression of Hsp70 on Glioma Cells

doi: 10.3390/cells9040912

Figure Lengend Snippet: Dose- and time-dependent radiation effects on the mean fluorescence intensity (mfi) of mHsp70 on U87 human glioblastoma cells over 7 days with different radiation doses (2 Gy ( A ), 4 Gy ( B ), 6 Gy ( C )). Bars represent the mean values and the corresponding standard deviation (SD) of n = 3 independent experiments. Significance (comparison of one dose on different days): * p ≤ 0.05. For comparison of different days at a single dose ANOVA has been conducted. Comparisons to sham (0 Gy) treated cells were performed by pairwise t -tests with pooled SD and Bonferroni correction for multiple testing. ( D ) Summary of the kinetics of the mHsp70 density after sham (0 Gy), 2, 4, 6 Gy) over a period of 7 days. For a better clarity, statistical significance was only shown in A–C, but not in D.

Article Snippet: The epithelial human glioblastoma cell line U87 MG (ATCC HTB-14, MGMT - ) and the human hepatocellular carcinoma cell line HepG2 (ATCC HB-8065) were grown in complete growth medium, consisting of Dulbecco’s Eagle’s Minimum Essential Medium (DMEM) (Sigma-Aldrich, Steinheim Germany) supplemented with 10% v / v heat inactivated fetal calf serum (FCS) (Sigma-Aldrich), 1% antibiotics (10,000 IU/mL penicillin, 10 mg/mL streptomycin, Sigma-Aldrich), l -glutamine (Sigma-Aldrich), MEM non-essential amino acid solution 100× (Sigma-Aldrich) and sodium pyruvate (Sigma-Aldrich).The epithelial human cervix carcinoma cell line HeLa (ATCC CCL-2) was grown in complete growth medium, consisting of RPMI-1640 (Sigma-Aldrich, Germany) supplemented with 10% v / v heat inactivated FCS (Sigma-Aldrich), 1% antibiotics (10,000 IU/mL penicillin, 10 mg/mL streptomycin, Sigma-Aldrich), l -glutamine (Sigma-Aldrich) and sodium pyruvate (Sigma-Aldrich).

Techniques: Fluorescence, Standard Deviation