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tyramide azide  (Iris Biotech Gmbh)

 
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    Structured Review

    Iris Biotech Gmbh tyramide azide
    Msm expressing APEX2, Sec-APEX2 or Tat-APEX2 was grown without or with theophylline and subjected to the labeling protocol with either <t>tyramide</t> <t>azide</t> or tyramide alkyne. Lysates underwent a CuAAC reaction with alkyne or azide-conjugated fluorescein. In-gel fluorescence detection of the clicked lysates was used to assess protein labeling. Equal protein loading was confirmed by subsequent Coomassie staining (Figure S5). Data are representative of 3 biological replicates under the specified conditions.
    Tyramide Azide, supplied by Iris Biotech Gmbh, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tyramide azide/product/Iris Biotech Gmbh
    Average 86 stars, based on 1 article reviews
    tyramide azide - by Bioz Stars, 2025-07
    86/100 stars

    Images

    1) Product Images from "Compartment-Specific Labeling of Bacterial Periplasmic Proteins by Peroxidase-Mediated Biotinylation"

    Article Title: Compartment-Specific Labeling of Bacterial Periplasmic Proteins by Peroxidase-Mediated Biotinylation

    Journal: ACS infectious diseases

    doi: 10.1021/acsinfecdis.8b00044

    Msm expressing APEX2, Sec-APEX2 or Tat-APEX2 was grown without or with theophylline and subjected to the labeling protocol with either tyramide azide or tyramide alkyne. Lysates underwent a CuAAC reaction with alkyne or azide-conjugated fluorescein. In-gel fluorescence detection of the clicked lysates was used to assess protein labeling. Equal protein loading was confirmed by subsequent Coomassie staining (Figure S5). Data are representative of 3 biological replicates under the specified conditions.
    Figure Legend Snippet: Msm expressing APEX2, Sec-APEX2 or Tat-APEX2 was grown without or with theophylline and subjected to the labeling protocol with either tyramide azide or tyramide alkyne. Lysates underwent a CuAAC reaction with alkyne or azide-conjugated fluorescein. In-gel fluorescence detection of the clicked lysates was used to assess protein labeling. Equal protein loading was confirmed by subsequent Coomassie staining (Figure S5). Data are representative of 3 biological replicates under the specified conditions.

    Techniques Used: Expressing, Labeling, Fluorescence, Staining



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    Iris Biotech Gmbh tyramide azide
    Msm expressing APEX2, Sec-APEX2 or Tat-APEX2 was grown without or with theophylline and subjected to the labeling protocol with either <t>tyramide</t> <t>azide</t> or tyramide alkyne. Lysates underwent a CuAAC reaction with alkyne or azide-conjugated fluorescein. In-gel fluorescence detection of the clicked lysates was used to assess protein labeling. Equal protein loading was confirmed by subsequent Coomassie staining (Figure S5). Data are representative of 3 biological replicates under the specified conditions.
    Tyramide Azide, supplied by Iris Biotech Gmbh, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tyramide azide/product/Iris Biotech Gmbh
    Average 86 stars, based on 1 article reviews
    tyramide azide - by Bioz Stars, 2025-07
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Msm expressing APEX2, Sec-APEX2 or Tat-APEX2 was grown without or with theophylline and subjected to the labeling protocol with either tyramide azide or tyramide alkyne. Lysates underwent a CuAAC reaction with alkyne or azide-conjugated fluorescein. In-gel fluorescence detection of the clicked lysates was used to assess protein labeling. Equal protein loading was confirmed by subsequent Coomassie staining (Figure S5). Data are representative of 3 biological replicates under the specified conditions.

    Journal: ACS infectious diseases

    Article Title: Compartment-Specific Labeling of Bacterial Periplasmic Proteins by Peroxidase-Mediated Biotinylation

    doi: 10.1021/acsinfecdis.8b00044

    Figure Lengend Snippet: Msm expressing APEX2, Sec-APEX2 or Tat-APEX2 was grown without or with theophylline and subjected to the labeling protocol with either tyramide azide or tyramide alkyne. Lysates underwent a CuAAC reaction with alkyne or azide-conjugated fluorescein. In-gel fluorescence detection of the clicked lysates was used to assess protein labeling. Equal protein loading was confirmed by subsequent Coomassie staining (Figure S5). Data are representative of 3 biological replicates under the specified conditions.

    Article Snippet: Biotin-phenol (Iris Biotech), tyramide azide and tyramide alkyne were prepared as 50 mM stocks in DMSO and used in cultures at 1 mM final concentration (final DMSO concentration was 2%).

    Techniques: Expressing, Labeling, Fluorescence, Staining