me raw milk atcc 11842 atcc type strain s sanguinis sk36 wild type e coli top10 cloning  (ATCC)


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    ATCC me raw milk atcc 11842 atcc type strain s sanguinis sk36 wild type e coli top10 cloning
    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis <t>SK36</t> on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    Me Raw Milk Atcc 11842 Atcc Type Strain S Sanguinis Sk36 Wild Type E Coli Top10 Cloning, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/me raw milk atcc 11842 atcc type strain s sanguinis sk36 wild type e coli top10 cloning/product/ATCC
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    Images

    1) Product Images from "Interspecies Inhibition of Porphyromonas gingivalis by Yogurt-Derived Lactobacillus delbrueckii Requires Active Pyruvate Oxidase"

    Article Title: Interspecies Inhibition of Porphyromonas gingivalis by Yogurt-Derived Lactobacillus delbrueckii Requires Active Pyruvate Oxidase

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01271-19

    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis SK36 on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    Figure Legend Snippet: Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis SK36 on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.

    Techniques Used:

    Bacterial strains and plasmids used in the study
    Figure Legend Snippet: Bacterial strains and plasmids used in the study

    Techniques Used: Plasmid Preparation, Mutagenesis, Isolation, Clone Assay, Conjugation Assay, Expressing

    me raw milk atcc 11842 atcc type strain s sanguinis sk36 wild type e coli top10 cloning  (ATCC)


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    ATCC me raw milk atcc 11842 atcc type strain s sanguinis sk36 wild type e coli top10 cloning
    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis <t>SK36</t> on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    Me Raw Milk Atcc 11842 Atcc Type Strain S Sanguinis Sk36 Wild Type E Coli Top10 Cloning, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/me raw milk atcc 11842 atcc type strain s sanguinis sk36 wild type e coli top10 cloning/product/ATCC
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    Images

    1) Product Images from "Interspecies Inhibition of Porphyromonas gingivalis by Yogurt-Derived Lactobacillus delbrueckii Requires Active Pyruvate Oxidase"

    Article Title: Interspecies Inhibition of Porphyromonas gingivalis by Yogurt-Derived Lactobacillus delbrueckii Requires Active Pyruvate Oxidase

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01271-19

    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis SK36 on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    Figure Legend Snippet: Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis SK36 on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.

    Techniques Used:

    Bacterial strains and plasmids used in the study
    Figure Legend Snippet: Bacterial strains and plasmids used in the study

    Techniques Used: Plasmid Preparation, Mutagenesis, Isolation, Clone Assay, Conjugation Assay, Expressing

    me raw milk atcc 11842 atcc type strain streptococcus sanguinis sk36 wild type escherichia coli top10 cloning  (ATCC)


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    ATCC me raw milk atcc 11842 atcc type strain streptococcus sanguinis sk36 wild type escherichia coli top10 cloning
    Me Raw Milk Atcc 11842 Atcc Type Strain Streptococcus Sanguinis Sk36 Wild Type Escherichia Coli Top10 Cloning, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/me raw milk atcc 11842 atcc type strain streptococcus sanguinis sk36 wild type escherichia coli top10 cloning/product/ATCC
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    type strain l brevis atcc 11842  (ATCC)


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    ATCC type strain l brevis atcc 11842
    Type Strain L Brevis Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    type strain atcc 11842  (ATCC)


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    ATCC type strain atcc 11842
    Type Strain Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    type strain atcc 11842  (ATCC)


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    ATCC type strain atcc 11842
    Type Strain Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    l delbrueckii subsp bulgaricus type strain atcc 11842  (ATCC)


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    ATCC l delbrueckii subsp bulgaricus type strain atcc 11842
    L Delbrueckii Subsp Bulgaricus Type Strain Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    type strain atcc 11842  (ATCC)


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    ATCC type strain atcc 11842
    Type Strain Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    l delbrueckii subsp bulgaricus type strain atcc 11842  (ATCC)


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    ATCC l delbrueckii subsp bulgaricus type strain atcc 11842
    Implication of protein synthesis in the viability of L. <t>delbrueckii</t> subsp. bulgaricus strain 546 during heat shock at 65°C. Cells in exponential phase were centrifuged, resuspended in MPL medium in the absence (▪) or in the presence (○) of 10 μg of chloramphenicol/ml, and incubated at room temperature for 20 min before heat shock at 65°C for the indicated times. Viability was monitored by plate counting during the challenge. Each point is the average of at least 11 independent experiments. Standard deviations are shown by bars.
    L Delbrueckii Subsp Bulgaricus Type Strain Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Two-Dimensional Electrophoresis Study of Lactobacillus delbrueckii subsp. bulgaricus Thermotolerance"

    Article Title: Two-Dimensional Electrophoresis Study of Lactobacillus delbrueckii subsp. bulgaricus Thermotolerance

    Journal:

    doi: 10.1128/AEM.68.3.1055-1063.2002

    Implication of protein synthesis in the viability of L. delbrueckii subsp. bulgaricus strain 546 during heat shock at 65°C. Cells in exponential phase were centrifuged, resuspended in MPL medium in the absence (▪) or in the presence (○) of 10 μg of chloramphenicol/ml, and incubated at room temperature for 20 min before heat shock at 65°C for the indicated times. Viability was monitored by plate counting during the challenge. Each point is the average of at least 11 independent experiments. Standard deviations are shown by bars.
    Figure Legend Snippet: Implication of protein synthesis in the viability of L. delbrueckii subsp. bulgaricus strain 546 during heat shock at 65°C. Cells in exponential phase were centrifuged, resuspended in MPL medium in the absence (▪) or in the presence (○) of 10 μg of chloramphenicol/ml, and incubated at room temperature for 20 min before heat shock at 65°C for the indicated times. Viability was monitored by plate counting during the challenge. Each point is the average of at least 11 independent experiments. Standard deviations are shown by bars.

    Techniques Used: Incubation

    PFGE patterns of NotI digests of genomic DNAs from L. delbrueckii subsp. bulgaricus strains. Lanes 1 and 6, molecular size standards; lane 2, strain ATCC 11842; lane 3, strain 546; lane 4, strain V5221; lane 5, strain V5242.
    Figure Legend Snippet: PFGE patterns of NotI digests of genomic DNAs from L. delbrueckii subsp. bulgaricus strains. Lanes 1 and 6, molecular size standards; lane 2, strain ATCC 11842; lane 3, strain 546; lane 4, strain V5221; lane 5, strain V5242.

    Techniques Used:

    Effects of heat and osmotic pretreatments on the thermotolerance of L. delbrueckii subsp. bulgaricus strains 546, V5221, and V5242. Exponential-phase cells were centrifuged, resuspended in MPL medium, and incubated for 10 min at 65°C without pretreatment (black columns), after treatment at 50°C for 30 min (white columns), or after treatment for 30 min with 0.4 mol of NaCl/liter (gray columns). Each point is the average of at least five independent experiments. Standard deviations are shown by bars.
    Figure Legend Snippet: Effects of heat and osmotic pretreatments on the thermotolerance of L. delbrueckii subsp. bulgaricus strains 546, V5221, and V5242. Exponential-phase cells were centrifuged, resuspended in MPL medium, and incubated for 10 min at 65°C without pretreatment (black columns), after treatment at 50°C for 30 min (white columns), or after treatment for 30 min with 0.4 mol of NaCl/liter (gray columns). Each point is the average of at least five independent experiments. Standard deviations are shown by bars.

    Techniques Used: Incubation

    Viability of L. delbrueckii subsp. bulgaricus strains 546 (○), V5221 (□), and V5242 (▵) during heat shock at 65°C. Exponential-phase cells were centrifuged, recovered in MPL medium, and incubated at 65°C. Each point is the average of at least five independent experiments. Standard deviations are shown by bars.
    Figure Legend Snippet: Viability of L. delbrueckii subsp. bulgaricus strains 546 (○), V5221 (□), and V5242 (▵) during heat shock at 65°C. Exponential-phase cells were centrifuged, recovered in MPL medium, and incubated at 65°C. Each point is the average of at least five independent experiments. Standard deviations are shown by bars.

    Techniques Used: Incubation

    Protein patterns determined for L. delbrueckii subsp. bulgaricus strains 546 and V5221 by 2-DE imaging of [35S]methionine-labeled polypeptides from both strains in exponential growth phase. Cells were grown in MPL medium, centrifuged, and resuspended in MPL medium. (A and B) Control conditions for strains 546 and V5221, respectively. (C and D) Protein patterns after heat shock for strains 546 and V5221, respectively. Cells were grown and prepared in the same manner as for the controls, except that they were heated at 65°C for 15 min. Abscissa, pH range; ordinate, kilodaltons. Symbols: ▵, proteins induced in strain 546 after heat shock at 65°C for 15 min (HSPs); □, newly synthesized proteins (NS1, NS2, and NS3) in strain 546 after heat shock; ○, proteins up-regulated in strain V5221 and down-regulated in strain 546 after heat shock; private-char pc1, proteins down-regulated during thermal stress; ◊, protein remaining unchanged during thermal stress.
    Figure Legend Snippet: Protein patterns determined for L. delbrueckii subsp. bulgaricus strains 546 and V5221 by 2-DE imaging of [35S]methionine-labeled polypeptides from both strains in exponential growth phase. Cells were grown in MPL medium, centrifuged, and resuspended in MPL medium. (A and B) Control conditions for strains 546 and V5221, respectively. (C and D) Protein patterns after heat shock for strains 546 and V5221, respectively. Cells were grown and prepared in the same manner as for the controls, except that they were heated at 65°C for 15 min. Abscissa, pH range; ordinate, kilodaltons. Symbols: ▵, proteins induced in strain 546 after heat shock at 65°C for 15 min (HSPs); □, newly synthesized proteins (NS1, NS2, and NS3) in strain 546 after heat shock; ○, proteins up-regulated in strain V5221 and down-regulated in strain 546 after heat shock; private-char pc1, proteins down-regulated during thermal stress; ◊, protein remaining unchanged during thermal stress.

    Techniques Used: Imaging, Labeling, Synthesized

    Sequences of heat-induced proteins from  L. delbrueckii subsp. bulgaricus strain  546
    Figure Legend Snippet: Sequences of heat-induced proteins from L. delbrueckii subsp. bulgaricus strain 546

    Techniques Used: Sequencing

    l delbrueckii subsp bulgaricus type strain atcc 11842  (ATCC)


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    ATCC l delbrueckii subsp bulgaricus type strain atcc 11842
    L Delbrueckii Subsp Bulgaricus Type Strain Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC me raw milk atcc 11842 atcc type strain s sanguinis sk36 wild type e coli top10 cloning
    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis <t>SK36</t> on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    Me Raw Milk Atcc 11842 Atcc Type Strain S Sanguinis Sk36 Wild Type E Coli Top10 Cloning, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC me raw milk atcc 11842 atcc type strain streptococcus sanguinis sk36 wild type escherichia coli top10 cloning
    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis <t>SK36</t> on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    Me Raw Milk Atcc 11842 Atcc Type Strain Streptococcus Sanguinis Sk36 Wild Type Escherichia Coli Top10 Cloning, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/me raw milk atcc 11842 atcc type strain streptococcus sanguinis sk36 wild type escherichia coli top10 cloning/product/ATCC
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    ATCC type strain l brevis atcc 11842
    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis <t>SK36</t> on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    Type Strain L Brevis Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC type strain atcc 11842
    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis <t>SK36</t> on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    Type Strain Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/type strain atcc 11842/product/ATCC
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    ATCC l delbrueckii subsp bulgaricus type strain atcc 11842
    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis <t>SK36</t> on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.
    L Delbrueckii Subsp Bulgaricus Type Strain Atcc 11842, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis SK36 on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.

    Journal: Applied and Environmental Microbiology

    Article Title: Interspecies Inhibition of Porphyromonas gingivalis by Yogurt-Derived Lactobacillus delbrueckii Requires Active Pyruvate Oxidase

    doi: 10.1128/AEM.01271-19

    Figure Lengend Snippet: Inhibitory strains produce more hydrogen peroxide more rapidly upon exposure to oxygen. Shown are images of each L. delbrueckii strain and S. sanguinis SK36 on Prussian blue agar plates after exposure to oxygen at 2 and 20 h after exposure. Dilutions of hydrogen peroxide are also shown. Shown are the mean measurements of the Prussian blue diameter from three independent experiments. Error bars represent standard errors of the mean. Statistical significance was determined by a one-way ANOVA corrected for multiple comparisons (*, P < 0.001). The asterisk indicates comparison and statistical significance between the asterisked bar and each of the SYB7, SYB13, 11842, and SK36 bars at the same time point.

    Article Snippet: Ampicillin (100 μg/ml; Fisher) was used when appropriate in LB agar or broth (Fisher). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain or plasmid Description or purpose a Strains P. gingivalis W83 Clinical isolate, also known as ATCC BAA-308 Tn-FeoB2 W83 background feoB2 (PG1294) transposon mutant; Erm r Tn-FeoB2 Comp Complemented Tn-FeoB2 with pT-COW_FeoB2; Erm r Tet r Tn-FeoB2 Empty Complemented Tn-FeoB2 with pT-COW; Erm r Tet r L. delbrueckii STYM1 Wild-type inhibitor strain isolated from Stonyfield Greek yogurt GVKM1 Wild-type inhibitor strain isolated from Green Valley Farm kefir SYB7 Wild-type noninhibitor strain isolated from Smith Family Farm, ME raw milk SYB13 Wild-type noninhibitor strain isolated from Smith Family Farm, ME raw milk ATCC 11842 ATCC type strain S. sanguinis SK36 Wild type E. coli Top10 Cloning and plasmid maintenance S17-1λ pir Conjugation into P. gingivalis DH5α Cloning and plasmid maintenance LOBSTR Low background strain for protein expression/purification Plasmids pT-COW Complementation vector without insert; Tet r Amp r pT-COW_FeoB2 pT-COW with wild-type W83 feoB2 and promoter pFLAG-CTC_1487-Pox E. coli expression plasmid with His 6 -tagged pyruvate oxidase (LDBND_1487); Amp r pFLAG-CTC_2051-Pox E. coli expression plasmid with His 6 -tagged pyruvate oxidase (LDBND_2051); Amp r Open in a separate window a Erm r , erythromycin resistance; Tet r , tetracycline resistance; Amp r , ampicillin resistance.

    Techniques:

    Bacterial strains and plasmids used in the study

    Journal: Applied and Environmental Microbiology

    Article Title: Interspecies Inhibition of Porphyromonas gingivalis by Yogurt-Derived Lactobacillus delbrueckii Requires Active Pyruvate Oxidase

    doi: 10.1128/AEM.01271-19

    Figure Lengend Snippet: Bacterial strains and plasmids used in the study

    Article Snippet: Ampicillin (100 μg/ml; Fisher) was used when appropriate in LB agar or broth (Fisher). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain or plasmid Description or purpose a Strains P. gingivalis W83 Clinical isolate, also known as ATCC BAA-308 Tn-FeoB2 W83 background feoB2 (PG1294) transposon mutant; Erm r Tn-FeoB2 Comp Complemented Tn-FeoB2 with pT-COW_FeoB2; Erm r Tet r Tn-FeoB2 Empty Complemented Tn-FeoB2 with pT-COW; Erm r Tet r L. delbrueckii STYM1 Wild-type inhibitor strain isolated from Stonyfield Greek yogurt GVKM1 Wild-type inhibitor strain isolated from Green Valley Farm kefir SYB7 Wild-type noninhibitor strain isolated from Smith Family Farm, ME raw milk SYB13 Wild-type noninhibitor strain isolated from Smith Family Farm, ME raw milk ATCC 11842 ATCC type strain S. sanguinis SK36 Wild type E. coli Top10 Cloning and plasmid maintenance S17-1λ pir Conjugation into P. gingivalis DH5α Cloning and plasmid maintenance LOBSTR Low background strain for protein expression/purification Plasmids pT-COW Complementation vector without insert; Tet r Amp r pT-COW_FeoB2 pT-COW with wild-type W83 feoB2 and promoter pFLAG-CTC_1487-Pox E. coli expression plasmid with His 6 -tagged pyruvate oxidase (LDBND_1487); Amp r pFLAG-CTC_2051-Pox E. coli expression plasmid with His 6 -tagged pyruvate oxidase (LDBND_2051); Amp r Open in a separate window a Erm r , erythromycin resistance; Tet r , tetracycline resistance; Amp r , ampicillin resistance.

    Techniques: Plasmid Preparation, Mutagenesis, Isolation, Clone Assay, Conjugation Assay, Expressing