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DSMZ type strain e coli k12 mg1655
The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli <t>K12</t> <t>MG1655</t> was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.
Type Strain E Coli K12 Mg1655, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors"

Article Title: Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors

Journal: Microorganisms

doi: 10.3390/microorganisms11020275

The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli K12 MG1655 was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.
Figure Legend Snippet: The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli K12 MG1655 was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.

Techniques Used: Concentration Assay, Derivative Assay

The relative fitness of replicate ALE experiments with E. coli K12 MG1655 growing with the non-native carbon source glycerol. Relative fitness is defined as the stable specific growth rate divided by the average stable specific growth rate of the control group of WT E. coli without NTG. The cumulative number of cell divisions (CCD) is used as time scale to measure adaptation progress. The specific growth rate is considered stable if the moving average of three consecutive batches has an absolute standard deviation < 0.01 h − 1 and no further upwards trend. This definition of a stable phenotype is specific to this set of experiments. A decisive criterion was required to make near-real-time decisions while the experiment was running; hence, a variability based approach was chosen that focuses on the change in optimization metric: the specific growth rate. The specific growth rate of the stable phenotype of the E. coli wild-type cultures without NTG is 0.61 ± 0.03 h − 1 at a l o g 10 ( C C D ) = 14.09 ± 0.09 and is used to compare both groups and calculate the relative fitness (grey shaded area, relative fitness of the WT = 1 ± 0.05 ). The observed average specific growth rate of the WT strain with NTG is 0.70 ± 0.05 h − 1 and was reached at a l o g 10 ( C C D ) = 14.39 ± 0.04 (orange shaded area, relative fitness of the WT with NTG = 1.15 ± 0.08 ).
Figure Legend Snippet: The relative fitness of replicate ALE experiments with E. coli K12 MG1655 growing with the non-native carbon source glycerol. Relative fitness is defined as the stable specific growth rate divided by the average stable specific growth rate of the control group of WT E. coli without NTG. The cumulative number of cell divisions (CCD) is used as time scale to measure adaptation progress. The specific growth rate is considered stable if the moving average of three consecutive batches has an absolute standard deviation < 0.01 h − 1 and no further upwards trend. This definition of a stable phenotype is specific to this set of experiments. A decisive criterion was required to make near-real-time decisions while the experiment was running; hence, a variability based approach was chosen that focuses on the change in optimization metric: the specific growth rate. The specific growth rate of the stable phenotype of the E. coli wild-type cultures without NTG is 0.61 ± 0.03 h − 1 at a l o g 10 ( C C D ) = 14.09 ± 0.09 and is used to compare both groups and calculate the relative fitness (grey shaded area, relative fitness of the WT = 1 ± 0.05 ). The observed average specific growth rate of the WT strain with NTG is 0.70 ± 0.05 h − 1 and was reached at a l o g 10 ( C C D ) = 14.39 ± 0.04 (orange shaded area, relative fitness of the WT with NTG = 1.15 ± 0.08 ).

Techniques Used: Standard Deviation



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DSMZ type strain e coli k12 mg1655
The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli <t>K12</t> <t>MG1655</t> was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.
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https://www.bioz.com/result/type strain e coli k12 mg1655/product/DSMZ
Average 86 stars, based on 1 article reviews
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86/100 stars
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The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli K12 MG1655 was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.

Journal: Microorganisms

Article Title: Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors

doi: 10.3390/microorganisms11020275

Figure Lengend Snippet: The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli K12 MG1655 was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.

Article Snippet: The experiments were carried out using fresh cultures of the wild-type strain E. coli K12 MG1655 from the German Collection of Microorganisms and Cell Cultures (#DSM 18039, DSMZ GmbH, Braunschweig, Germany).

Techniques: Concentration Assay, Derivative Assay

The relative fitness of replicate ALE experiments with E. coli K12 MG1655 growing with the non-native carbon source glycerol. Relative fitness is defined as the stable specific growth rate divided by the average stable specific growth rate of the control group of WT E. coli without NTG. The cumulative number of cell divisions (CCD) is used as time scale to measure adaptation progress. The specific growth rate is considered stable if the moving average of three consecutive batches has an absolute standard deviation < 0.01 h − 1 and no further upwards trend. This definition of a stable phenotype is specific to this set of experiments. A decisive criterion was required to make near-real-time decisions while the experiment was running; hence, a variability based approach was chosen that focuses on the change in optimization metric: the specific growth rate. The specific growth rate of the stable phenotype of the E. coli wild-type cultures without NTG is 0.61 ± 0.03 h − 1 at a l o g 10 ( C C D ) = 14.09 ± 0.09 and is used to compare both groups and calculate the relative fitness (grey shaded area, relative fitness of the WT = 1 ± 0.05 ). The observed average specific growth rate of the WT strain with NTG is 0.70 ± 0.05 h − 1 and was reached at a l o g 10 ( C C D ) = 14.39 ± 0.04 (orange shaded area, relative fitness of the WT with NTG = 1.15 ± 0.08 ).

Journal: Microorganisms

Article Title: Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors

doi: 10.3390/microorganisms11020275

Figure Lengend Snippet: The relative fitness of replicate ALE experiments with E. coli K12 MG1655 growing with the non-native carbon source glycerol. Relative fitness is defined as the stable specific growth rate divided by the average stable specific growth rate of the control group of WT E. coli without NTG. The cumulative number of cell divisions (CCD) is used as time scale to measure adaptation progress. The specific growth rate is considered stable if the moving average of three consecutive batches has an absolute standard deviation < 0.01 h − 1 and no further upwards trend. This definition of a stable phenotype is specific to this set of experiments. A decisive criterion was required to make near-real-time decisions while the experiment was running; hence, a variability based approach was chosen that focuses on the change in optimization metric: the specific growth rate. The specific growth rate of the stable phenotype of the E. coli wild-type cultures without NTG is 0.61 ± 0.03 h − 1 at a l o g 10 ( C C D ) = 14.09 ± 0.09 and is used to compare both groups and calculate the relative fitness (grey shaded area, relative fitness of the WT = 1 ± 0.05 ). The observed average specific growth rate of the WT strain with NTG is 0.70 ± 0.05 h − 1 and was reached at a l o g 10 ( C C D ) = 14.39 ± 0.04 (orange shaded area, relative fitness of the WT with NTG = 1.15 ± 0.08 ).

Article Snippet: The experiments were carried out using fresh cultures of the wild-type strain E. coli K12 MG1655 from the German Collection of Microorganisms and Cell Cultures (#DSM 18039, DSMZ GmbH, Braunschweig, Germany).

Techniques: Standard Deviation