Structured Review

DSMZ type strain e coli k12 mg1655
The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli <t>K12</t> <t>MG1655</t> was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.
Type Strain E Coli K12 Mg1655, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/type strain e coli k12 mg1655/product/DSMZ
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
type strain e coli k12 mg1655 - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors"

Article Title: Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors

Journal: Microorganisms

doi: 10.3390/microorganisms11020275

The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli K12 MG1655 was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.
Figure Legend Snippet: The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli K12 MG1655 was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.

Techniques Used: Concentration Assay, Derivative Assay

The relative fitness of replicate ALE experiments with E. coli K12 MG1655 growing with the non-native carbon source glycerol. Relative fitness is defined as the stable specific growth rate divided by the average stable specific growth rate of the control group of WT E. coli without NTG. The cumulative number of cell divisions (CCD) is used as time scale to measure adaptation progress. The specific growth rate is considered stable if the moving average of three consecutive batches has an absolute standard deviation < 0.01 h − 1 and no further upwards trend. This definition of a stable phenotype is specific to this set of experiments. A decisive criterion was required to make near-real-time decisions while the experiment was running; hence, a variability based approach was chosen that focuses on the change in optimization metric: the specific growth rate. The specific growth rate of the stable phenotype of the E. coli wild-type cultures without NTG is 0.61 ± 0.03 h − 1 at a l o g 10 ( C C D ) = 14.09 ± 0.09 and is used to compare both groups and calculate the relative fitness (grey shaded area, relative fitness of the WT = 1 ± 0.05 ). The observed average specific growth rate of the WT strain with NTG is 0.70 ± 0.05 h − 1 and was reached at a l o g 10 ( C C D ) = 14.39 ± 0.04 (orange shaded area, relative fitness of the WT with NTG = 1.15 ± 0.08 ).
Figure Legend Snippet: The relative fitness of replicate ALE experiments with E. coli K12 MG1655 growing with the non-native carbon source glycerol. Relative fitness is defined as the stable specific growth rate divided by the average stable specific growth rate of the control group of WT E. coli without NTG. The cumulative number of cell divisions (CCD) is used as time scale to measure adaptation progress. The specific growth rate is considered stable if the moving average of three consecutive batches has an absolute standard deviation < 0.01 h − 1 and no further upwards trend. This definition of a stable phenotype is specific to this set of experiments. A decisive criterion was required to make near-real-time decisions while the experiment was running; hence, a variability based approach was chosen that focuses on the change in optimization metric: the specific growth rate. The specific growth rate of the stable phenotype of the E. coli wild-type cultures without NTG is 0.61 ± 0.03 h − 1 at a l o g 10 ( C C D ) = 14.09 ± 0.09 and is used to compare both groups and calculate the relative fitness (grey shaded area, relative fitness of the WT = 1 ± 0.05 ). The observed average specific growth rate of the WT strain with NTG is 0.70 ± 0.05 h − 1 and was reached at a l o g 10 ( C C D ) = 14.39 ± 0.04 (orange shaded area, relative fitness of the WT with NTG = 1.15 ± 0.08 ).

Techniques Used: Standard Deviation


Structured Review

DSMZ type e coli mg1655
Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli <t>MG1655</t> and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
Type E Coli Mg1655, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/type e coli mg1655/product/DSMZ
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
type e coli mg1655 - by Bioz Stars, 2024-04
96/100 stars

Images

1) Product Images from "A Transcription Factor-Based Biosensor for Detection of Itaconic Acid"

Article Title: A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

Journal: ACS Synthetic Biology

doi: 10.1021/acssynbio.8b00057

Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli MG1655 and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
Figure Legend Snippet: Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli MG1655 and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

Techniques Used: Expressing, Fluorescence, Plasmid Preparation, Negative Control

Kinetics and dynamics of the Yp ItcR/P ccl inducible system. (A) Absolute normalized fluorescence of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system (pEH086) in response to different concentrations of itaconate added at time zero. The standard deviation of three biological replicates is shown as a lighter color ribbon displayed lengthwise of the induction kinetics curve. For the lower concentrations, the standard deviation is too small to be visible. (B) Dose response curve of the Yp ItcR/P ccl inducible system in E. coli MG1655, illustrating the correlation between inducer concentration and fluorescence output 4 and 8 h post-induction (hpi) with itaconate. Error bars represent standard deviations of three biological replicates.
Figure Legend Snippet: Kinetics and dynamics of the Yp ItcR/P ccl inducible system. (A) Absolute normalized fluorescence of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system (pEH086) in response to different concentrations of itaconate added at time zero. The standard deviation of three biological replicates is shown as a lighter color ribbon displayed lengthwise of the induction kinetics curve. For the lower concentrations, the standard deviation is too small to be visible. (B) Dose response curve of the Yp ItcR/P ccl inducible system in E. coli MG1655, illustrating the correlation between inducer concentration and fluorescence output 4 and 8 h post-induction (hpi) with itaconate. Error bars represent standard deviations of three biological replicates.

Techniques Used: Fluorescence, Standard Deviation, Concentration Assay

Inducer-dependent orthogonality of the Yp ItcR/P ccl inducible system. (A) Compounds that were investigated for cross-induction with the Yp ItcR/P ccl inducible system: itaconic acid (1), succinic acid (2), d -malic acid (3), l -malic acid (4), fumaric acid (5), oxaloacetic acid (6), l -aspartic acid (7), methylsuccinic acid (8), mesaconic acid (9), citraconic acid (10), α-ketoglutaric acid (11), l -glutamic acid (12), acetic acid (13), propionic acid (14), butyric acid (15), 3-butenoic acid (16), valeric acid (17), acrylic acid (18), methacrylic acid (19), tiglic acid (20), citric acid (21), cis -aconitic acid (22), trans -aconitic acid (23), tricarballylic acid (24), isocitric acid (25). (B) Normalized fluorescence (in %) of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system 12 hours after addition of different compounds at a final concentration of 5 mM, relative to the fluorescence output obtained by adding 5 mM itaconate. (−) uninduced sample. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
Figure Legend Snippet: Inducer-dependent orthogonality of the Yp ItcR/P ccl inducible system. (A) Compounds that were investigated for cross-induction with the Yp ItcR/P ccl inducible system: itaconic acid (1), succinic acid (2), d -malic acid (3), l -malic acid (4), fumaric acid (5), oxaloacetic acid (6), l -aspartic acid (7), methylsuccinic acid (8), mesaconic acid (9), citraconic acid (10), α-ketoglutaric acid (11), l -glutamic acid (12), acetic acid (13), propionic acid (14), butyric acid (15), 3-butenoic acid (16), valeric acid (17), acrylic acid (18), methacrylic acid (19), tiglic acid (20), citric acid (21), cis -aconitic acid (22), trans -aconitic acid (23), tricarballylic acid (24), isocitric acid (25). (B) Normalized fluorescence (in %) of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system 12 hours after addition of different compounds at a final concentration of 5 mM, relative to the fluorescence output obtained by adding 5 mM itaconate. (−) uninduced sample. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

Techniques Used: Fluorescence, Concentration Assay

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    DSMZ type strain e coli k12 mg1655
    The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli <t>K12</t> <t>MG1655</t> was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.
    Type Strain E Coli K12 Mg1655, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/type strain e coli k12 mg1655/product/DSMZ
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    type strain e coli k12 mg1655 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

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    DSMZ type e coli mg1655
    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli <t>MG1655</t> and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
    Type E Coli Mg1655, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/type e coli mg1655/product/DSMZ
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    type e coli mg1655 - by Bioz Stars, 2024-04
    96/100 stars
      Buy from Supplier

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    The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli K12 MG1655 was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.

    Journal: Microorganisms

    Article Title: Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors

    doi: 10.3390/microorganisms11020275

    Figure Lengend Snippet: The concentrations of O 2 and C O 2 in the off-gas of an automated ALE experiment (GM2) in an L scale stirred-tank bioreactor are shown over the course of 25 consecutive batch experiments totaling a process duration of 200 h. A culture of E. coli K12 MG1655 was grown with RB medium at 37 °C, 600–1400 rpm, 40 vvm, and an initial glycerol concentration of 12 g L − 1 as sole carbon source. The ALE process was performed in an automated system in a repeated batch mode with a bioreactor volume of 575 mL. Vertical lines indicate the start and end of the medium exchange procedure between batches (grey). The concentrations of O 2 = 20.91 % and C O 2 = 0.04 % in the pressurized air in the inflow are depicted by the horizontal, dashed lines (black). Batch numbers are indicated with B4–B24. The shown data were used as input for the black box model to calculate OUR and CER, as well as derived state variables, such as the estimated biomass and substrate concentrations and the specific growth rate according to Equation –.

    Article Snippet: The experiments were carried out using fresh cultures of the wild-type strain E. coli K12 MG1655 from the German Collection of Microorganisms and Cell Cultures (#DSM 18039, DSMZ GmbH, Braunschweig, Germany).

    Techniques: Concentration Assay, Derivative Assay

    The relative fitness of replicate ALE experiments with E. coli K12 MG1655 growing with the non-native carbon source glycerol. Relative fitness is defined as the stable specific growth rate divided by the average stable specific growth rate of the control group of WT E. coli without NTG. The cumulative number of cell divisions (CCD) is used as time scale to measure adaptation progress. The specific growth rate is considered stable if the moving average of three consecutive batches has an absolute standard deviation < 0.01 h − 1 and no further upwards trend. This definition of a stable phenotype is specific to this set of experiments. A decisive criterion was required to make near-real-time decisions while the experiment was running; hence, a variability based approach was chosen that focuses on the change in optimization metric: the specific growth rate. The specific growth rate of the stable phenotype of the E. coli wild-type cultures without NTG is 0.61 ± 0.03 h − 1 at a l o g 10 ( C C D ) = 14.09 ± 0.09 and is used to compare both groups and calculate the relative fitness (grey shaded area, relative fitness of the WT = 1 ± 0.05 ). The observed average specific growth rate of the WT strain with NTG is 0.70 ± 0.05 h − 1 and was reached at a l o g 10 ( C C D ) = 14.39 ± 0.04 (orange shaded area, relative fitness of the WT with NTG = 1.15 ± 0.08 ).

    Journal: Microorganisms

    Article Title: Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized Bioreactors

    doi: 10.3390/microorganisms11020275

    Figure Lengend Snippet: The relative fitness of replicate ALE experiments with E. coli K12 MG1655 growing with the non-native carbon source glycerol. Relative fitness is defined as the stable specific growth rate divided by the average stable specific growth rate of the control group of WT E. coli without NTG. The cumulative number of cell divisions (CCD) is used as time scale to measure adaptation progress. The specific growth rate is considered stable if the moving average of three consecutive batches has an absolute standard deviation < 0.01 h − 1 and no further upwards trend. This definition of a stable phenotype is specific to this set of experiments. A decisive criterion was required to make near-real-time decisions while the experiment was running; hence, a variability based approach was chosen that focuses on the change in optimization metric: the specific growth rate. The specific growth rate of the stable phenotype of the E. coli wild-type cultures without NTG is 0.61 ± 0.03 h − 1 at a l o g 10 ( C C D ) = 14.09 ± 0.09 and is used to compare both groups and calculate the relative fitness (grey shaded area, relative fitness of the WT = 1 ± 0.05 ). The observed average specific growth rate of the WT strain with NTG is 0.70 ± 0.05 h − 1 and was reached at a l o g 10 ( C C D ) = 14.39 ± 0.04 (orange shaded area, relative fitness of the WT with NTG = 1.15 ± 0.08 ).

    Article Snippet: The experiments were carried out using fresh cultures of the wild-type strain E. coli K12 MG1655 from the German Collection of Microorganisms and Cell Cultures (#DSM 18039, DSMZ GmbH, Braunschweig, Germany).

    Techniques: Standard Deviation

    Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli MG1655 and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

    Journal: ACS Synthetic Biology

    Article Title: A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

    doi: 10.1021/acssynbio.8b00057

    Figure Lengend Snippet: Influence of ItcR on inducible gene expression. Absolute normalized fluorescence (in arbitrary units) of (A) E. coli MG1655 and (B) C. necator H16 harboring the Y. pseudotuberculosis ( Yp ) and P. aeruginosa ( Pa ) itaconate-inducible systems composed of promoter and transcriptional regulator (ItcR/P), and promoter-only (P) implementation in the absence and presence of 5 mM itaconate. Single time-point fluorescence measurements were taken 6 h after inducer addition. The promoterless reporter plasmid pEH006E was employed as negative control. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

    Article Snippet: RFP fluorescence assays for biosensor characterization were performed in wild type E. coli MG1655 (DSMZ 18039) and C. necator H16 (ATCC 17699).

    Techniques: Expressing, Fluorescence, Plasmid Preparation, Negative Control

    Kinetics and dynamics of the Yp ItcR/P ccl inducible system. (A) Absolute normalized fluorescence of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system (pEH086) in response to different concentrations of itaconate added at time zero. The standard deviation of three biological replicates is shown as a lighter color ribbon displayed lengthwise of the induction kinetics curve. For the lower concentrations, the standard deviation is too small to be visible. (B) Dose response curve of the Yp ItcR/P ccl inducible system in E. coli MG1655, illustrating the correlation between inducer concentration and fluorescence output 4 and 8 h post-induction (hpi) with itaconate. Error bars represent standard deviations of three biological replicates.

    Journal: ACS Synthetic Biology

    Article Title: A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

    doi: 10.1021/acssynbio.8b00057

    Figure Lengend Snippet: Kinetics and dynamics of the Yp ItcR/P ccl inducible system. (A) Absolute normalized fluorescence of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system (pEH086) in response to different concentrations of itaconate added at time zero. The standard deviation of three biological replicates is shown as a lighter color ribbon displayed lengthwise of the induction kinetics curve. For the lower concentrations, the standard deviation is too small to be visible. (B) Dose response curve of the Yp ItcR/P ccl inducible system in E. coli MG1655, illustrating the correlation between inducer concentration and fluorescence output 4 and 8 h post-induction (hpi) with itaconate. Error bars represent standard deviations of three biological replicates.

    Article Snippet: RFP fluorescence assays for biosensor characterization were performed in wild type E. coli MG1655 (DSMZ 18039) and C. necator H16 (ATCC 17699).

    Techniques: Fluorescence, Standard Deviation, Concentration Assay

    Inducer-dependent orthogonality of the Yp ItcR/P ccl inducible system. (A) Compounds that were investigated for cross-induction with the Yp ItcR/P ccl inducible system: itaconic acid (1), succinic acid (2), d -malic acid (3), l -malic acid (4), fumaric acid (5), oxaloacetic acid (6), l -aspartic acid (7), methylsuccinic acid (8), mesaconic acid (9), citraconic acid (10), α-ketoglutaric acid (11), l -glutamic acid (12), acetic acid (13), propionic acid (14), butyric acid (15), 3-butenoic acid (16), valeric acid (17), acrylic acid (18), methacrylic acid (19), tiglic acid (20), citric acid (21), cis -aconitic acid (22), trans -aconitic acid (23), tricarballylic acid (24), isocitric acid (25). (B) Normalized fluorescence (in %) of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system 12 hours after addition of different compounds at a final concentration of 5 mM, relative to the fluorescence output obtained by adding 5 mM itaconate. (−) uninduced sample. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

    Journal: ACS Synthetic Biology

    Article Title: A Transcription Factor-Based Biosensor for Detection of Itaconic Acid

    doi: 10.1021/acssynbio.8b00057

    Figure Lengend Snippet: Inducer-dependent orthogonality of the Yp ItcR/P ccl inducible system. (A) Compounds that were investigated for cross-induction with the Yp ItcR/P ccl inducible system: itaconic acid (1), succinic acid (2), d -malic acid (3), l -malic acid (4), fumaric acid (5), oxaloacetic acid (6), l -aspartic acid (7), methylsuccinic acid (8), mesaconic acid (9), citraconic acid (10), α-ketoglutaric acid (11), l -glutamic acid (12), acetic acid (13), propionic acid (14), butyric acid (15), 3-butenoic acid (16), valeric acid (17), acrylic acid (18), methacrylic acid (19), tiglic acid (20), citric acid (21), cis -aconitic acid (22), trans -aconitic acid (23), tricarballylic acid (24), isocitric acid (25). (B) Normalized fluorescence (in %) of E. coli MG1655 harboring the Yp ItcR/P ccl inducible system 12 hours after addition of different compounds at a final concentration of 5 mM, relative to the fluorescence output obtained by adding 5 mM itaconate. (−) uninduced sample. Error bars represent standard deviations of three biological replicates. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).

    Article Snippet: RFP fluorescence assays for biosensor characterization were performed in wild type E. coli MG1655 (DSMZ 18039) and C. necator H16 (ATCC 17699).

    Techniques: Fluorescence, Concentration Assay