Article Title: Quantification of Bacterial Twitching Motility in Dense Colonies Using Transmitted Light Microscopy and Computational Image Analysis
Figure Lengend Snippet: Experimental setup and images of the twitching assay A. Schematic showing the experimental setup. Twitching medium (~60 °C) is poured onto a glass slide and allowed to cool and dry in a biosafety cabinet (BSL2) for 20 min. After adding the substance to be tested, the slide is dried again in the biosafety cabinet for 15 min before inoculation with bacteria. A coverslip is then placed over the inoculated substance to be tested, and the slide incubated for 4 h at 37 °C in a moist environment. B. Example of an inoculated slide with coverslip showing three circles, within each a replicate of the substance being tested. C. Example of a large Petri dish containing four inoculated slides with coverslips ( e.g. , a control slide versus three slides comparing different substances, or different concentrations of the same substance). Two wet paper towels are included to prevent drying of the twitching medium during the 4 h incubation. Although not shown, the Petri dish is covered with its lid during incubation to maintain moisture.
Article Snippet: Tweezers (Dumont, Dumoxel N°5, Fine Science Tools, catalog number: 11252-30) Bunsen burner 60 °C oven (Boekel Scientific, model: 133000) Sterile Biosafety Cabinet (NUAIRE, model: NU-425-600) Compound microscope with a high NA objective and digital camera (≥ 1.2).
Techniques: Hood, Incubation