tween20  (Millipore)


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    Name:
    TWEEN 20
    Description:

    Catalog Number:
    p5927
    Price:
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    Applications:
    Non-ionic detergent
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    Structured Review

    Millipore tween20
    TWEEN 20

    https://www.bioz.com/result/tween20/product/Millipore
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    tween20 - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Immunocytochemistry:

    Article Title: Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis
    Article Snippet: Paragraph title: Immunocytochemistry ... Three washes (10 min) with PBS-T (0.05 % Tween-20, P1379-250ML, Sigma Aldrich) were performed.

    Immunostaining:

    Article Title: BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair
    Article Snippet: Paragraph title: Immunostaining ... After three washes in 0.1% Tween20/PBS (PBST) (Tween20, Sigma, P7949), sections were incubated with a commercial blocking reagent (Abcam, ab126587) for 1 h followed by a primary antibody overnight at 4 °C.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Molecular approach to describing a seed-based food web: the post-dispersal granivore community of an invasive plant
    Article Snippet: Paragraph title: ELISA protocols ... The milk was ejected, and the plate was washed three times with PBS-Tween (PBST; Tween® 20, Product number P1379, Sigma-Aldrich).

    Sandwich ELISA:

    Article Title: Molecular approach to describing a seed-based food web: the post-dispersal granivore community of an invasive plant
    Article Snippet: The specimens were vortexed, solids were centrifuged out over 3 min at 16,100 rcf, and samples were subjected to double-antibody sandwich ELISA. .. The milk was ejected, and the plate was washed three times with PBS-Tween (PBST; Tween® 20, Product number P1379, Sigma-Aldrich).

    Incubation:

    Article Title: METTL3 and ALKBH5 oppositely regulate m6A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes
    Article Snippet: .. TBST (0.1% Tween 20 [Sigma-Aldrich, P9416] plus TBS [50 mM Tris-Cl, pH 7.5, 150 mM NaCl]) buffer including 1% non-fat milk was used to block the membranes and then was incubated overnight at 4°C using the indicated antibodies. .. After being washed three times with TBST, membranes were incubated with an indicated concentration of HRP-conjugated secondary antibodies (Thermo Fisher Scientific, 32230, 32460) for 1 h in the blocking solution at room temperature.

    Article Title: Membrane Lipid Screen to Identify Molecular Targets of Biomolecules
    Article Snippet: .. Small gel incubation tray (Santa Cruz Biotechnology, catalog number: sc-358889) Serological pipets, 10 ml (Genesee Scientific, catalog number: 12-104) Pipette tips 10 μl tips (VWR, catalog number: 46620-318) 200 μl tips (VWR, catalog number: 53509-009) 1,000 μl tips (VWR, catalog number: 83007-384) Centrifuge tubes, 50 ml (Genesee Scientific, catalog number: 21-106) Microcentrifuge tubes, 1.7 ml (MIDSCI, catalog number: AVSS1700RA) Membrane lipid strips with dimensions 2×3 cm (Echelon Biosciences, catalog number: P-6002) Protein or biomolecule of interest Tris (Gold Bio, catalog number: T-400-5) Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S9888-25KG) Tween 20% (Sigma-Aldrich, catalog number: P1379) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A7906-500G) Primary antibody against the tagged protein or biomolecule of interest Peroxidase conjugated secondary antibody ECL Prime Western Blotting Detection Kit (GE Healthcare, catalog number: RPN2232) Phospholipids (dissolved in chloroform, commonly used phospholipids are): DOPC (Avanti Polar Lipids, catalog number: 850375C) POPC (Avanti Polar Lipids, catalog number: 850457C) POPA (Avanti Polar Lipids, catalog number: 840857C) POPS (Avanti Polar Lipids, catalog number: 840034C) Blocking buffer (see Recipes) Wash buffer (see Recipes) Note: See the ‘Notes’ section for a list of materials and reagents used in the companion protocol ‘Liposome disruption assay to examine lytic properties of biomolecules’ that is recommended for follow up experiments ( ). .. Pipetman Classic pipets P10 (Gilson, catalog number: F144802) P20 (Gilson, catalog number: F123600) P200 (Gilson, catalog number: F123601) P1000 (Gilson, catalog number: F123602) Pipet-Aid XP Pipette controller (Drummond Scientific, catalog number: 4-000-101) Incubator BenchRocker™ variable 2D rocker (Benchmark Scientific, catalog number: BR2000) pH meter (Fisher Scientific, model: Accumet™ AE150, catalog number: 13-636-AE153) Fluorescent image analyzer (Fujifilm, model: FLA-5000) Note: See the ‘Notes’ section for a list of equipment used in the companion protocol ‘Liposome disruption assay to examine lytic properties of biomolecules’ that is recommended for follow up experiments ( ).

    Article Title: Contractile activity attenuates autophagy suppression and reverses mitochondrial defects in skeletal muscle cells
    Article Snippet: .. Membranes were blocked in 5% skim milk in TBST buffer (100 mM TRIS, 100 mM NaCl, 0.1% Tween 20 [Sigma, P1379]) for 1 h, and then incubated overnight at 4°C with primary antibodies directed against COX4I1 (1:1000; Abcam, ab14744), SOD2 (1:000; Upstate Biotechnology, 06–984), BNIP3L/NIX (1:500; Abcam, ab109414), LC3B (1:1000; Cell Signaling Technology, 2775), SQSTM1/p62 (1:40,0000; Sigma-Aldrich, P0067), PGM5/ACICULIN (1:500; in house), VDAC (1:5000; Abcam, ab1473), PPARGC1A/PGC1α (1:1000; Millipore Corporation, ab3243), p-RPS6KB (1:1000, Cell Signaling Technology, 9202), pan-LAMP2 (abcam, ab13524; 1:1000), CTSD (1:1000; Santa Cruz Biotechnology, SC6486), Tubulin (1:90,000, Calbiochem, CP06), ACTB (1:1000, Santa Cruz Biotechnology, 47,778) and TFEB (1:1000; MyBioSource, mbs120432). ..

    Article Title: BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair
    Article Snippet: .. After three washes in 0.1% Tween20/PBS (PBST) (Tween20, Sigma, P7949), sections were incubated with a commercial blocking reagent (Abcam, ab126587) for 1 h followed by a primary antibody overnight at 4 °C. .. Sections were washed three times in PBST and then incubated with the Alexa-conjugated secondary antibody (Invitrogen, A-11008).

    Article Title: Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis. Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis
    Article Snippet: Seminiferous tubules stored in 1× PBS for whole‐mount immunofluorescence were washed in 0.2% Nonidet P40 Substitute (74 385, Sigma‐Aldrich)/1× PBS for 20 min. A graded methanol series (25%, 50%, 75%, 100%) in PBS‐T (PBS containing 0.1% Tween 20 (P1379, Sigma‐Aldrich) for 5 min each was performed to dehydrate the tubules. .. The tubules were then incubated with the TH2B primary antibody diluted in blocking solution overnight at 4°C with gentle rotation.

    Article Title: Tolerogenic Nanoparticles Induce Antigen-Specific Regulatory T Cells and Provide Therapeutic Efficacy and Transferrable Tolerance against Experimental Autoimmune Encephalomyelitis
    Article Snippet: .. The plates were washed three times with wash buffer (WB) [PBS containing 0.05% Tween20 (Sigma P1379)] after which the plates were incubated for 90 min at room temperature with 2µg/ml biotinylated anti-mouse IFNγ detection antibody (BD Pharmingen 551881) diluted in DB. .. The plates were washed three times with WB followed by Stretavidin-HRP (BD Pharmingen 557630) addition diluted 1:100 in DB and incubated for 1 h at room temperature.

    Expressing:

    Article Title: Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption
    Article Snippet: Equal amounts of 50 μg protein were resolved in SDS-PAGE, then transferred to PVDF membrane (ThermoFisher Scientific, Waltham, MA, Product number 88520) and blocked with 5% milk in PBST (0.1% Tween 20, Sigma-Aldrich, St. Louis, MO, P1379-500ml) for 1 hour at room temperature. .. Finally, protein expression was determined by treating the membrane with Clarity Western ECL substrate (Bio-Rad, Hercules, CA, Category number 1705060) and measuring luminescence with an Amersham Imager 600 system (GE Healthcare, Waukesha, WI). (All antibody information and working concentrations are shown in ).

    BIA-KA:

    Article Title: METTL3 and ALKBH5 oppositely regulate m6A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes
    Article Snippet: TBST (0.1% Tween 20 [Sigma-Aldrich, P9416] plus TBS [50 mM Tris-Cl, pH 7.5, 150 mM NaCl]) buffer including 1% non-fat milk was used to block the membranes and then was incubated overnight at 4°C using the indicated antibodies. .. TBST (0.1% Tween 20 [Sigma-Aldrich, P9416] plus TBS [50 mM Tris-Cl, pH 7.5, 150 mM NaCl]) buffer including 1% non-fat milk was used to block the membranes and then was incubated overnight at 4°C using the indicated antibodies.

    Article Title: Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption
    Article Snippet: After 10-cycle of sonication (Bioruptor Pico Sonication System, Diagenode, Denville, NJ, Category Number B01060001), proteins were extracted, and the concentration was determined by a BCA kit (Pierce, Rockford, IL; 23228). .. Equal amounts of 50 μg protein were resolved in SDS-PAGE, then transferred to PVDF membrane (ThermoFisher Scientific, Waltham, MA, Product number 88520) and blocked with 5% milk in PBST (0.1% Tween 20, Sigma-Aldrich, St. Louis, MO, P1379-500ml) for 1 hour at room temperature.

    Modification:

    Article Title: Method of Bottom-Up Directed Assembly of Cell-Laden Microgels
    Article Snippet: .. Poly(ethylene glycol)-dimethacrylate polymer 1000 (Polysciences, Inc., cat. no. 15178) Dulbecco's Phosphate Buffered Saline (DPBS) (Gibco, cat. no. 14190) 1% photoinitiator (2-hydroxy-1-[4-(hydroxy-ethoxy) phenyl]-2-methyl-1-propanone) (Ciba Chemicals, Irgacure 2959) Mineral Oil (CVS Pharmacy) Tween 20 (surfactant) (Sigma, cat. no. P-5927) FITC-dextran (MW = 2000 kDa) (Sigma, cat. no. FD2000S) Rhodamine-dextran (MW = 10 kDa) (Sigma, cat. no. R8881) Nile red (MW = 317 kDa) (Sigma, cat. no. N3013) Green fluorescent microbeads (1% solid, D = 5 μ m) (Duke Scientific, G0500) NIH 3T3 mouse fibroblasts Dulbecco's Modified Eagle Medium (Gibco, cat. no. 11965) 10% FBS (to supplement DMEM) (Gibco, cat. no. 26140) 0.5% Trypsin-EDTA 10× (Gibco, cat. no. 15400) Live/Dead dyes: Calcein AM and Ethidium Homodimer-1 (Molecular Probes, cat. no. L3224) PKH26 Red Fluorescent Cell Linker (Sigma, cat. no. PKH26GL-1KT) .. Photomasks with different patterns (i.e. rectangular or lock-and-key) designed using AutoCAD and printed on transparencies with 20,000-dpi resolution (CAD/Art Services) Micro cover glasses (150 μ m thick, 18 × 18 mm) (VWR, cat. no. 48366 045) UV light (The OmniCure® S2000 UV/Visible Spot Curing System) 60 × 15-mm dish (Fisher Scientific, cat. no. 430589) 10 μ L pipette tips 15-mL tubes (BD Biosciences, cat. no. 352096) Vortexer Centrifuge Incubator (5% CO2 at 37 °C) Microscope (with 4× and 10× objective lenses)

    Article Title: Microporous methacrylated glycol chitosan-montmorillonite nanocomposite hydrogel for bone tissue engineering
    Article Snippet: MMT (682659-500G), glycidyl methacrylate (GMA, 151238-100G), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC, E1769-10G), tween-20 (p1379-500ML), p -nitrophenyl phosphate (N7653-100ML), β-glycerophosphate (G5422-100G), L -ascorbic acid (A5960-25G), dexamethasone (D4902-25MG), Nitro Blue Tetrazolium (NBT, N5514-25TAB), 5-bromo-4-chloro-3-indoxylphosphate (BCIP, B6149-50MG), alizarin red S (A5533), and ethylenediaminetetraacetic acid (EDTA, EDS-1KG) were supplied by Sigma-Aldrich (St. Louis, MO, USA). .. High glucose Dulbecco’s Modified Eagle’s Medium (DMEM, 11995-065), penicillin/streptomycin (100 U/mL, 15140122) were purchased from Life Technologies (Grand Island, NY, USA), and fetal bovine serum (FBS, MT35010CV) was supplied by Mediatech Inc. (Manassas, VA, USA).

    Article Title: N-(2-Aminoethyl) Ethanolamine-Induced Morphological, Biochemical, and Biophysical Alterations in Vascular Matrix Associated With Dissecting Aortic Aneurysm
    Article Snippet: Urea extraction buffer was modified based on the “4 × LOX-buffer” , which contains 4.8 M urea, 200 mM sodium phosphate buffer, pH 7.4. .. Trizma base (T-8524), sodium deoxycholate (D6750), sodium phosphate dibasic (S0876), sodium phosphate mono basic (S0751), urea (U5128), MTT (M2128), Tween 20 (P9416), and albumin bovine serum (BSA, A9647) were purchased from Sigma.

    Western Blot:

    Article Title: Membrane Lipid Screen to Identify Molecular Targets of Biomolecules
    Article Snippet: .. Small gel incubation tray (Santa Cruz Biotechnology, catalog number: sc-358889) Serological pipets, 10 ml (Genesee Scientific, catalog number: 12-104) Pipette tips 10 μl tips (VWR, catalog number: 46620-318) 200 μl tips (VWR, catalog number: 53509-009) 1,000 μl tips (VWR, catalog number: 83007-384) Centrifuge tubes, 50 ml (Genesee Scientific, catalog number: 21-106) Microcentrifuge tubes, 1.7 ml (MIDSCI, catalog number: AVSS1700RA) Membrane lipid strips with dimensions 2×3 cm (Echelon Biosciences, catalog number: P-6002) Protein or biomolecule of interest Tris (Gold Bio, catalog number: T-400-5) Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S9888-25KG) Tween 20% (Sigma-Aldrich, catalog number: P1379) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A7906-500G) Primary antibody against the tagged protein or biomolecule of interest Peroxidase conjugated secondary antibody ECL Prime Western Blotting Detection Kit (GE Healthcare, catalog number: RPN2232) Phospholipids (dissolved in chloroform, commonly used phospholipids are): DOPC (Avanti Polar Lipids, catalog number: 850375C) POPC (Avanti Polar Lipids, catalog number: 850457C) POPA (Avanti Polar Lipids, catalog number: 840857C) POPS (Avanti Polar Lipids, catalog number: 840034C) Blocking buffer (see Recipes) Wash buffer (see Recipes) Note: See the ‘Notes’ section for a list of materials and reagents used in the companion protocol ‘Liposome disruption assay to examine lytic properties of biomolecules’ that is recommended for follow up experiments ( ). .. Pipetman Classic pipets P10 (Gilson, catalog number: F144802) P20 (Gilson, catalog number: F123600) P200 (Gilson, catalog number: F123601) P1000 (Gilson, catalog number: F123602) Pipet-Aid XP Pipette controller (Drummond Scientific, catalog number: 4-000-101) Incubator BenchRocker™ variable 2D rocker (Benchmark Scientific, catalog number: BR2000) pH meter (Fisher Scientific, model: Accumet™ AE150, catalog number: 13-636-AE153) Fluorescent image analyzer (Fujifilm, model: FLA-5000) Note: See the ‘Notes’ section for a list of equipment used in the companion protocol ‘Liposome disruption assay to examine lytic properties of biomolecules’ that is recommended for follow up experiments ( ).

    Article Title: Blastocystis specific serum immunoglobulin in patients with irritable bowel syndrome (IBS) versus healthy controls
    Article Snippet: A separate single protein standard ladder well in the gel was loaded with 15uL of Precision Plus Protein Western C Standard (Bio-Rad: 161–0385). .. The membrane was rinsed three times with 0.1 % 1Mphosphate buffered saline (PBS)/Tween 20 (PBST) (Sigma Aldrich, St Louis, USA: P5927) solution and then blocked in 100 ml of blocking solution (0.1 % PBST, 4g skim milk and1g bovine serum albumin per 100 ml (Sigma Aldrich: A2153) for 60 min at room temperature.

    Article Title: Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption
    Article Snippet: Paragraph title: Western blot analysis ... Equal amounts of 50 μg protein were resolved in SDS-PAGE, then transferred to PVDF membrane (ThermoFisher Scientific, Waltham, MA, Product number 88520) and blocked with 5% milk in PBST (0.1% Tween 20, Sigma-Aldrich, St. Louis, MO, P1379-500ml) for 1 hour at room temperature.

    Article Title: Tolerogenic Nanoparticles Induce Antigen-Specific Regulatory T Cells and Provide Therapeutic Efficacy and Transferrable Tolerance against Experimental Autoimmune Encephalomyelitis
    Article Snippet: .. The plates were washed three times with wash buffer (WB) [PBS containing 0.05% Tween20 (Sigma P1379)] after which the plates were incubated for 90 min at room temperature with 2µg/ml biotinylated anti-mouse IFNγ detection antibody (BD Pharmingen 551881) diluted in DB. .. The plates were washed three times with WB followed by Stretavidin-HRP (BD Pharmingen 557630) addition diluted 1:100 in DB and incubated for 1 h at room temperature.

    Electron Microscopy:

    Article Title: Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis
    Article Snippet: Immunocytochemistry Cells were grown on Matrigel-coated glass coverslips and fixed for 10–15 min with 4 % paraformaldehyde (15713, Electron Microscopy Sciences) in PBS (14040-117, ThermoFisher Scientific) and subsequently washed twice with PBS. .. Three washes (10 min) with PBS-T (0.05 % Tween-20, P1379-250ML, Sigma Aldrich) were performed.

    Protease Inhibitor:

    Article Title: Contractile activity attenuates autophagy suppression and reverses mitochondrial defects in skeletal muscle cells
    Article Snippet: Cultured myotubes were washed 2x with ice-cold PBS and scraped in Passive Lysis Buffer (Promega, E194A) supplemented with Complete Protease Inhibitor Cocktail Tablets (Roche, 11,697,498,001). .. Membranes were blocked in 5% skim milk in TBST buffer (100 mM TRIS, 100 mM NaCl, 0.1% Tween 20 [Sigma, P1379]) for 1 h, and then incubated overnight at 4°C with primary antibodies directed against COX4I1 (1:1000; Abcam, ab14744), SOD2 (1:000; Upstate Biotechnology, 06–984), BNIP3L/NIX (1:500; Abcam, ab109414), LC3B (1:1000; Cell Signaling Technology, 2775), SQSTM1/p62 (1:40,0000; Sigma-Aldrich, P0067), PGM5/ACICULIN (1:500; in house), VDAC (1:5000; Abcam, ab1473), PPARGC1A/PGC1α (1:1000; Millipore Corporation, ab3243), p-RPS6KB (1:1000, Cell Signaling Technology, 9202), pan-LAMP2 (abcam, ab13524; 1:1000), CTSD (1:1000; Santa Cruz Biotechnology, SC6486), Tubulin (1:90,000, Calbiochem, CP06), ACTB (1:1000, Santa Cruz Biotechnology, 47,778) and TFEB (1:1000; MyBioSource, mbs120432).

    Transferring:

    Article Title: Membrane Lipid Screen to Identify Molecular Targets of Biomolecules
    Article Snippet: .. Small gel incubation tray (Santa Cruz Biotechnology, catalog number: sc-358889) Serological pipets, 10 ml (Genesee Scientific, catalog number: 12-104) Pipette tips 10 μl tips (VWR, catalog number: 46620-318) 200 μl tips (VWR, catalog number: 53509-009) 1,000 μl tips (VWR, catalog number: 83007-384) Centrifuge tubes, 50 ml (Genesee Scientific, catalog number: 21-106) Microcentrifuge tubes, 1.7 ml (MIDSCI, catalog number: AVSS1700RA) Membrane lipid strips with dimensions 2×3 cm (Echelon Biosciences, catalog number: P-6002) Protein or biomolecule of interest Tris (Gold Bio, catalog number: T-400-5) Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S9888-25KG) Tween 20% (Sigma-Aldrich, catalog number: P1379) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A7906-500G) Primary antibody against the tagged protein or biomolecule of interest Peroxidase conjugated secondary antibody ECL Prime Western Blotting Detection Kit (GE Healthcare, catalog number: RPN2232) Phospholipids (dissolved in chloroform, commonly used phospholipids are): DOPC (Avanti Polar Lipids, catalog number: 850375C) POPC (Avanti Polar Lipids, catalog number: 850457C) POPA (Avanti Polar Lipids, catalog number: 840857C) POPS (Avanti Polar Lipids, catalog number: 840034C) Blocking buffer (see Recipes) Wash buffer (see Recipes) Note: See the ‘Notes’ section for a list of materials and reagents used in the companion protocol ‘Liposome disruption assay to examine lytic properties of biomolecules’ that is recommended for follow up experiments ( ). .. Pipetman Classic pipets P10 (Gilson, catalog number: F144802) P20 (Gilson, catalog number: F123600) P200 (Gilson, catalog number: F123601) P1000 (Gilson, catalog number: F123602) Pipet-Aid XP Pipette controller (Drummond Scientific, catalog number: 4-000-101) Incubator BenchRocker™ variable 2D rocker (Benchmark Scientific, catalog number: BR2000) pH meter (Fisher Scientific, model: Accumet™ AE150, catalog number: 13-636-AE153) Fluorescent image analyzer (Fujifilm, model: FLA-5000) Note: See the ‘Notes’ section for a list of equipment used in the companion protocol ‘Liposome disruption assay to examine lytic properties of biomolecules’ that is recommended for follow up experiments ( ).

    Cell Culture:

    Article Title: Contractile activity attenuates autophagy suppression and reverses mitochondrial defects in skeletal muscle cells
    Article Snippet: Cultured myotubes were washed 2x with ice-cold PBS and scraped in Passive Lysis Buffer (Promega, E194A) supplemented with Complete Protease Inhibitor Cocktail Tablets (Roche, 11,697,498,001). .. Membranes were blocked in 5% skim milk in TBST buffer (100 mM TRIS, 100 mM NaCl, 0.1% Tween 20 [Sigma, P1379]) for 1 h, and then incubated overnight at 4°C with primary antibodies directed against COX4I1 (1:1000; Abcam, ab14744), SOD2 (1:000; Upstate Biotechnology, 06–984), BNIP3L/NIX (1:500; Abcam, ab109414), LC3B (1:1000; Cell Signaling Technology, 2775), SQSTM1/p62 (1:40,0000; Sigma-Aldrich, P0067), PGM5/ACICULIN (1:500; in house), VDAC (1:5000; Abcam, ab1473), PPARGC1A/PGC1α (1:1000; Millipore Corporation, ab3243), p-RPS6KB (1:1000, Cell Signaling Technology, 9202), pan-LAMP2 (abcam, ab13524; 1:1000), CTSD (1:1000; Santa Cruz Biotechnology, SC6486), Tubulin (1:90,000, Calbiochem, CP06), ACTB (1:1000, Santa Cruz Biotechnology, 47,778) and TFEB (1:1000; MyBioSource, mbs120432).

    Sonication:

    Article Title: Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption
    Article Snippet: After 10-cycle of sonication (Bioruptor Pico Sonication System, Diagenode, Denville, NJ, Category Number B01060001), proteins were extracted, and the concentration was determined by a BCA kit (Pierce, Rockford, IL; 23228). .. Equal amounts of 50 μg protein were resolved in SDS-PAGE, then transferred to PVDF membrane (ThermoFisher Scientific, Waltham, MA, Product number 88520) and blocked with 5% milk in PBST (0.1% Tween 20, Sigma-Aldrich, St. Louis, MO, P1379-500ml) for 1 hour at room temperature.

    Recombinant:

    Article Title: Targeted quantitative mass spectrometric immunoassay for human protein variants
    Article Snippet: Recombinant human cystatin C was purchased from HyTest (Turku, Finland, Cat. No. 8CY5). .. Beta-lactoglobulin from bovine milk (Cat. No. L8005), 1,1' Carbonyldiimidazole (115533), TWEEN 20 (P7949), TRIS (T-6128), Dithiothreitol (DTT, 43815) and α-cyano -4-hydroxycinnamic acid (476870) were obtained from Sigma-Aldrich (St. Lous, MO).

    Immunofluorescence:

    Article Title: Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis. Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis
    Article Snippet: .. Seminiferous tubules stored in 1× PBS for whole‐mount immunofluorescence were washed in 0.2% Nonidet P40 Substitute (74 385, Sigma‐Aldrich)/1× PBS for 20 min. A graded methanol series (25%, 50%, 75%, 100%) in PBS‐T (PBS containing 0.1% Tween 20 (P1379, Sigma‐Aldrich) for 5 min each was performed to dehydrate the tubules. ..

    MTT Assay:

    Article Title: N-(2-Aminoethyl) Ethanolamine-Induced Morphological, Biochemical, and Biophysical Alterations in Vascular Matrix Associated With Dissecting Aortic Aneurysm
    Article Snippet: .. Trizma base (T-8524), sodium deoxycholate (D6750), sodium phosphate dibasic (S0876), sodium phosphate mono basic (S0751), urea (U5128), MTT (M2128), Tween 20 (P9416), and albumin bovine serum (BSA, A9647) were purchased from Sigma. .. Sodium chloride (Catalog # 7581) was from Mallinckrodt.

    Article Title: Targeting DNA Repair through Podophyllotoxin and Rutin Formulation in Hematopoietic Radioprotection: An in Silico, in Vitro, and in Vivo Study
    Article Snippet: .. Reagents and Cells Podophyllotoxin (344192), rutin (R5143), sodium chloride (S3014), potassium chloride (P9541), sodium phosphate dibasic (S3264), potassium phosphate monobasic (P9791), potassium bicarbonate (12602), ammonium chloride (A9434), ethylene di amine tetra acetic acid di sodium salt (E6635), paraformaldehyde (158127), poly-L -lysine (P8920), tween 20 (P9416), bovine serum albumin (BSA) (5482), ribonuclease A from bovine pancreas (R6513), propidium iodide solution (P4864), triton X-100 (T8787), DCF-DA (D6883), MTT (T8787), and ethidium bromide solution (E1510) were purchased from Sigma–Aldrich, St. Louis, MO, United States. .. Dimethyl sulfoxide (DMSO) (16743.0521), methanol (1060091000), ethanol (100983), anti-phospho-histone monoclonal γH2AX Ser 139 (05-636), anti-phospho-ATM (Ser1981) (05-740), Anti-hMre 11 (PC388), Anti-Rad 50 (07-1781) Anti Anti-Cyclin B1 PE (FCMAB102P), goat anti-mouse IgG (H + L) fluorescein-conjugated (AP308F), and goat anti-rabbit IgG (H + L) fluorescein-conjugated (AP307F) were purchased from EMD Millipore, United States and Canada. p53 Binding Protein 1 antibody (PA1-46147), DNA PKcs Antibody (MA5-13404), versene solution (15040066) were purchased from Thermo scientific, United States.

    Size-exclusion Chromatography:

    Article Title: Molecular approach to describing a seed-based food web: the post-dispersal granivore community of an invasive plant
    Article Snippet: ELISA protocols Insects were thawed and surface sterilized for at least 30 sec in 70% ethanol. .. The milk was ejected, and the plate was washed three times with PBS-Tween (PBST; Tween® 20, Product number P1379, Sigma-Aldrich).

    Purification:

    Article Title: Tolerogenic Nanoparticles Induce Antigen-Specific Regulatory T Cells and Provide Therapeutic Efficacy and Transferrable Tolerance against Experimental Autoimmune Encephalomyelitis
    Article Snippet: Sterile, white 96-well filter plates with 0.45- µm pore size Hydrophobic PVDF membrane (EMD Millipore, Billerica, MA, USA, Cat#MSIPS4W10) were coated with 5µg/ml of purified anti-mouse IFNγ capture antibody (BD Pharmingen 551881) in dilution buffer (DB) (PBS 1× Corning cellgro 21-040CV) with 2% fetal bovine serum (FBS; heat inactivated, Medsupply partners MSP-1003-3/Hi) overnight at 4°C. .. The plates were washed three times with wash buffer (WB) [PBS containing 0.05% Tween20 (Sigma P1379)] after which the plates were incubated for 90 min at room temperature with 2µg/ml biotinylated anti-mouse IFNγ detection antibody (BD Pharmingen 551881) diluted in DB.

    Protein Extraction:

    Article Title: Contractile activity attenuates autophagy suppression and reverses mitochondrial defects in skeletal muscle cells
    Article Snippet: Paragraph title: Immunoblotting and protein extraction ... Membranes were blocked in 5% skim milk in TBST buffer (100 mM TRIS, 100 mM NaCl, 0.1% Tween 20 [Sigma, P1379]) for 1 h, and then incubated overnight at 4°C with primary antibodies directed against COX4I1 (1:1000; Abcam, ab14744), SOD2 (1:000; Upstate Biotechnology, 06–984), BNIP3L/NIX (1:500; Abcam, ab109414), LC3B (1:1000; Cell Signaling Technology, 2775), SQSTM1/p62 (1:40,0000; Sigma-Aldrich, P0067), PGM5/ACICULIN (1:500; in house), VDAC (1:5000; Abcam, ab1473), PPARGC1A/PGC1α (1:1000; Millipore Corporation, ab3243), p-RPS6KB (1:1000, Cell Signaling Technology, 9202), pan-LAMP2 (abcam, ab13524; 1:1000), CTSD (1:1000; Santa Cruz Biotechnology, SC6486), Tubulin (1:90,000, Calbiochem, CP06), ACTB (1:1000, Santa Cruz Biotechnology, 47,778) and TFEB (1:1000; MyBioSource, mbs120432).

    Blocking Assay:

    Article Title: METTL3 and ALKBH5 oppositely regulate m6A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes
    Article Snippet: .. TBST (0.1% Tween 20 [Sigma-Aldrich, P9416] plus TBS [50 mM Tris-Cl, pH 7.5, 150 mM NaCl]) buffer including 1% non-fat milk was used to block the membranes and then was incubated overnight at 4°C using the indicated antibodies. .. After being washed three times with TBST, membranes were incubated with an indicated concentration of HRP-conjugated secondary antibodies (Thermo Fisher Scientific, 32230, 32460) for 1 h in the blocking solution at room temperature.

    Article Title: Membrane Lipid Screen to Identify Molecular Targets of Biomolecules
    Article Snippet: .. Small gel incubation tray (Santa Cruz Biotechnology, catalog number: sc-358889) Serological pipets, 10 ml (Genesee Scientific, catalog number: 12-104) Pipette tips 10 μl tips (VWR, catalog number: 46620-318) 200 μl tips (VWR, catalog number: 53509-009) 1,000 μl tips (VWR, catalog number: 83007-384) Centrifuge tubes, 50 ml (Genesee Scientific, catalog number: 21-106) Microcentrifuge tubes, 1.7 ml (MIDSCI, catalog number: AVSS1700RA) Membrane lipid strips with dimensions 2×3 cm (Echelon Biosciences, catalog number: P-6002) Protein or biomolecule of interest Tris (Gold Bio, catalog number: T-400-5) Sodium chloride (NaCl) (Sigma-Aldrich, catalog number: S9888-25KG) Tween 20% (Sigma-Aldrich, catalog number: P1379) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A7906-500G) Primary antibody against the tagged protein or biomolecule of interest Peroxidase conjugated secondary antibody ECL Prime Western Blotting Detection Kit (GE Healthcare, catalog number: RPN2232) Phospholipids (dissolved in chloroform, commonly used phospholipids are): DOPC (Avanti Polar Lipids, catalog number: 850375C) POPC (Avanti Polar Lipids, catalog number: 850457C) POPA (Avanti Polar Lipids, catalog number: 840857C) POPS (Avanti Polar Lipids, catalog number: 840034C) Blocking buffer (see Recipes) Wash buffer (see Recipes) Note: See the ‘Notes’ section for a list of materials and reagents used in the companion protocol ‘Liposome disruption assay to examine lytic properties of biomolecules’ that is recommended for follow up experiments ( ). .. Pipetman Classic pipets P10 (Gilson, catalog number: F144802) P20 (Gilson, catalog number: F123600) P200 (Gilson, catalog number: F123601) P1000 (Gilson, catalog number: F123602) Pipet-Aid XP Pipette controller (Drummond Scientific, catalog number: 4-000-101) Incubator BenchRocker™ variable 2D rocker (Benchmark Scientific, catalog number: BR2000) pH meter (Fisher Scientific, model: Accumet™ AE150, catalog number: 13-636-AE153) Fluorescent image analyzer (Fujifilm, model: FLA-5000) Note: See the ‘Notes’ section for a list of equipment used in the companion protocol ‘Liposome disruption assay to examine lytic properties of biomolecules’ that is recommended for follow up experiments ( ).

    Article Title: Blastocystis specific serum immunoglobulin in patients with irritable bowel syndrome (IBS) versus healthy controls
    Article Snippet: .. The membrane was rinsed three times with 0.1 % 1Mphosphate buffered saline (PBS)/Tween 20 (PBST) (Sigma Aldrich, St Louis, USA: P5927) solution and then blocked in 100 ml of blocking solution (0.1 % PBST, 4g skim milk and1g bovine serum albumin per 100 ml (Sigma Aldrich: A2153) for 60 min at room temperature. .. Immunolabeling protocol Membrane strips were incubated with primary antibody (patient sera or MAbs) diluted in 1 mL of Signal Boost™Immunoreaction Enhancer Kit (Calbiochem, Darstadt, Germany: 407207) Solution One for 24 h on a rocker platform at 4 °C before washing consecutively three times for 15 min in PBST.

    Article Title: Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis
    Article Snippet: Permeabilization and blocking was performed using 10 % NDS (S30-100ML, Merck-Millipore), 0.5 % Triton X-100 (Sigma Aldrich), and 1 % BSA (15260037, ThermoFisher Scientific) in PBS for 20 min. Primary antibodies were applied overnight at 4°C in 3 % NDS in PBS. .. Three washes (10 min) with PBS-T (0.05 % Tween-20, P1379-250ML, Sigma Aldrich) were performed.

    Article Title: BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair
    Article Snippet: .. After three washes in 0.1% Tween20/PBS (PBST) (Tween20, Sigma, P7949), sections were incubated with a commercial blocking reagent (Abcam, ab126587) for 1 h followed by a primary antibody overnight at 4 °C. .. Sections were washed three times in PBST and then incubated with the Alexa-conjugated secondary antibody (Invitrogen, A-11008).

    Article Title: Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis. Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis
    Article Snippet: Seminiferous tubules stored in 1× PBS for whole‐mount immunofluorescence were washed in 0.2% Nonidet P40 Substitute (74 385, Sigma‐Aldrich)/1× PBS for 20 min. A graded methanol series (25%, 50%, 75%, 100%) in PBS‐T (PBS containing 0.1% Tween 20 (P1379, Sigma‐Aldrich) for 5 min each was performed to dehydrate the tubules. .. Nonspecific binding of the primary antibodies was inhibited by immersing the testis tubules in blocking solution (1% bovine serum albumin/10% donkey serum/PBS‐T) for 1 hr.

    Article Title: Tolerogenic Nanoparticles Induce Antigen-Specific Regulatory T Cells and Provide Therapeutic Efficacy and Transferrable Tolerance against Experimental Autoimmune Encephalomyelitis
    Article Snippet: Unbound antibodies were discarded by emptying the wells followed by blocking with complete medium (CM) [RPMI 1640 (1× with Corning glutagro, Mediatech 10-104-CV) supplemented with FBS (FBS, heat inactivated, Medsupply partners MSP-1003-3/Hi), Pen/Strep and l -glutamine (100×), Gibco by Life technologies 10378-016, 2-Mercaptoethanol (55 mM), Gibco by Life technologies 21985] for 90 min at 37°C. .. The plates were washed three times with wash buffer (WB) [PBS containing 0.05% Tween20 (Sigma P1379)] after which the plates were incubated for 90 min at room temperature with 2µg/ml biotinylated anti-mouse IFNγ detection antibody (BD Pharmingen 551881) diluted in DB.

    Lysis:

    Article Title: Contractile activity attenuates autophagy suppression and reverses mitochondrial defects in skeletal muscle cells
    Article Snippet: Cultured myotubes were washed 2x with ice-cold PBS and scraped in Passive Lysis Buffer (Promega, E194A) supplemented with Complete Protease Inhibitor Cocktail Tablets (Roche, 11,697,498,001). .. Membranes were blocked in 5% skim milk in TBST buffer (100 mM TRIS, 100 mM NaCl, 0.1% Tween 20 [Sigma, P1379]) for 1 h, and then incubated overnight at 4°C with primary antibodies directed against COX4I1 (1:1000; Abcam, ab14744), SOD2 (1:000; Upstate Biotechnology, 06–984), BNIP3L/NIX (1:500; Abcam, ab109414), LC3B (1:1000; Cell Signaling Technology, 2775), SQSTM1/p62 (1:40,0000; Sigma-Aldrich, P0067), PGM5/ACICULIN (1:500; in house), VDAC (1:5000; Abcam, ab1473), PPARGC1A/PGC1α (1:1000; Millipore Corporation, ab3243), p-RPS6KB (1:1000, Cell Signaling Technology, 9202), pan-LAMP2 (abcam, ab13524; 1:1000), CTSD (1:1000; Santa Cruz Biotechnology, SC6486), Tubulin (1:90,000, Calbiochem, CP06), ACTB (1:1000, Santa Cruz Biotechnology, 47,778) and TFEB (1:1000; MyBioSource, mbs120432).

    Article Title: Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption
    Article Snippet: Briefly, pancreata were homogenized in RIPA lysis buffer containing phosphatase and protease Inhibitors (GenDEPOT, Katy, TX; P3100-001 and P3200-001) on ice. .. Equal amounts of 50 μg protein were resolved in SDS-PAGE, then transferred to PVDF membrane (ThermoFisher Scientific, Waltham, MA, Product number 88520) and blocked with 5% milk in PBST (0.1% Tween 20, Sigma-Aldrich, St. Louis, MO, P1379-500ml) for 1 hour at room temperature.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Tolerogenic Nanoparticles Induce Antigen-Specific Regulatory T Cells and Provide Therapeutic Efficacy and Transferrable Tolerance against Experimental Autoimmune Encephalomyelitis
    Article Snippet: Sterile, white 96-well filter plates with 0.45- µm pore size Hydrophobic PVDF membrane (EMD Millipore, Billerica, MA, USA, CatMSIPS4W10) were coated with 5µg/ml of purified anti-mouse IFNγ capture antibody (BD Pharmingen 551881) in dilution buffer (DB) (PBS 1× Corning cellgro 21-040CV) with 2% fetal bovine serum (FBS; heat inactivated, Medsupply partners MSP-1003-3/Hi) overnight at 4°C. .. The plates were washed three times with wash buffer (WB) [PBS containing 0.05% Tween20 (Sigma P1379)] after which the plates were incubated for 90 min at room temperature with 2µg/ml biotinylated anti-mouse IFNγ detection antibody (BD Pharmingen 551881) diluted in DB.

    SDS Page:

    Article Title: METTL3 and ALKBH5 oppositely regulate m6A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes
    Article Snippet: Immunocomplexes were eluted in loading buffer and loaded onto a 7.5% SDS-PAGE gel and then transferred to polyvinylidene difluoride membranes (Millipore Sigma, IPVH00010). .. TBST (0.1% Tween 20 [Sigma-Aldrich, P9416] plus TBS [50 mM Tris-Cl, pH 7.5, 150 mM NaCl]) buffer including 1% non-fat milk was used to block the membranes and then was incubated overnight at 4°C using the indicated antibodies.

    Article Title: Contractile activity attenuates autophagy suppression and reverses mitochondrial defects in skeletal muscle cells
    Article Snippet: Whole cell and mitochondrial fraction extracts (20–40 μg of protein) were separated using 8–15% SDS-PAGE and then transferred to nitrocellulose membranes. .. Membranes were blocked in 5% skim milk in TBST buffer (100 mM TRIS, 100 mM NaCl, 0.1% Tween 20 [Sigma, P1379]) for 1 h, and then incubated overnight at 4°C with primary antibodies directed against COX4I1 (1:1000; Abcam, ab14744), SOD2 (1:000; Upstate Biotechnology, 06–984), BNIP3L/NIX (1:500; Abcam, ab109414), LC3B (1:1000; Cell Signaling Technology, 2775), SQSTM1/p62 (1:40,0000; Sigma-Aldrich, P0067), PGM5/ACICULIN (1:500; in house), VDAC (1:5000; Abcam, ab1473), PPARGC1A/PGC1α (1:1000; Millipore Corporation, ab3243), p-RPS6KB (1:1000, Cell Signaling Technology, 9202), pan-LAMP2 (abcam, ab13524; 1:1000), CTSD (1:1000; Santa Cruz Biotechnology, SC6486), Tubulin (1:90,000, Calbiochem, CP06), ACTB (1:1000, Santa Cruz Biotechnology, 47,778) and TFEB (1:1000; MyBioSource, mbs120432).

    Article Title: Blastocystis specific serum immunoglobulin in patients with irritable bowel syndrome (IBS) versus healthy controls
    Article Snippet: Paragraph title: SDS-PAGE protocol ... The membrane was rinsed three times with 0.1 % 1Mphosphate buffered saline (PBS)/Tween 20 (PBST) (Sigma Aldrich, St Louis, USA: P5927) solution and then blocked in 100 ml of blocking solution (0.1 % PBST, 4g skim milk and1g bovine serum albumin per 100 ml (Sigma Aldrich: A2153) for 60 min at room temperature.

    Article Title: Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption
    Article Snippet: .. Equal amounts of 50 μg protein were resolved in SDS-PAGE, then transferred to PVDF membrane (ThermoFisher Scientific, Waltham, MA, Product number 88520) and blocked with 5% milk in PBST (0.1% Tween 20, Sigma-Aldrich, St. Louis, MO, P1379-500ml) for 1 hour at room temperature. .. Finally, protein expression was determined by treating the membrane with Clarity Western ECL substrate (Bio-Rad, Hercules, CA, Category number 1705060) and measuring luminescence with an Amersham Imager 600 system (GE Healthcare, Waukesha, WI). (All antibody information and working concentrations are shown in ).

    Software:

    Article Title: Tolerogenic Nanoparticles Induce Antigen-Specific Regulatory T Cells and Provide Therapeutic Efficacy and Transferrable Tolerance against Experimental Autoimmune Encephalomyelitis
    Article Snippet: The plates were washed three times with wash buffer (WB) [PBS containing 0.05% Tween20 (Sigma P1379)] after which the plates were incubated for 90 min at room temperature with 2µg/ml biotinylated anti-mouse IFNγ detection antibody (BD Pharmingen 551881) diluted in DB. .. The plates were read on a Zeiss KS Elispot reader system, using KS Elispot software version 4.9.16.

    Binding Assay:

    Article Title: Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis. Differential localization of histone variant TH2B during the first round compared with subsequent rounds of spermatogenesis
    Article Snippet: Seminiferous tubules stored in 1× PBS for whole‐mount immunofluorescence were washed in 0.2% Nonidet P40 Substitute (74 385, Sigma‐Aldrich)/1× PBS for 20 min. A graded methanol series (25%, 50%, 75%, 100%) in PBS‐T (PBS containing 0.1% Tween 20 (P1379, Sigma‐Aldrich) for 5 min each was performed to dehydrate the tubules. .. Nonspecific binding of the primary antibodies was inhibited by immersing the testis tubules in blocking solution (1% bovine serum albumin/10% donkey serum/PBS‐T) for 1 hr.

    Article Title: Targeting DNA Repair through Podophyllotoxin and Rutin Formulation in Hematopoietic Radioprotection: An in Silico, in Vitro, and in Vivo Study
    Article Snippet: Reagents and Cells Podophyllotoxin (344192), rutin (R5143), sodium chloride (S3014), potassium chloride (P9541), sodium phosphate dibasic (S3264), potassium phosphate monobasic (P9791), potassium bicarbonate (12602), ammonium chloride (A9434), ethylene di amine tetra acetic acid di sodium salt (E6635), paraformaldehyde (158127), poly-L -lysine (P8920), tween 20 (P9416), bovine serum albumin (BSA) (5482), ribonuclease A from bovine pancreas (R6513), propidium iodide solution (P4864), triton X-100 (T8787), DCF-DA (D6883), MTT (T8787), and ethidium bromide solution (E1510) were purchased from Sigma–Aldrich, St. Louis, MO, United States. .. Dimethyl sulfoxide (DMSO) (16743.0521), methanol (1060091000), ethanol (100983), anti-phospho-histone monoclonal γH2AX Ser 139 (05-636), anti-phospho-ATM (Ser1981) (05-740), Anti-hMre 11 (PC388), Anti-Rad 50 (07-1781) Anti Anti-Cyclin B1 PE (FCMAB102P), goat anti-mouse IgG (H + L) fluorescein-conjugated (AP308F), and goat anti-rabbit IgG (H + L) fluorescein-conjugated (AP307F) were purchased from EMD Millipore, United States and Canada. p53 Binding Protein 1 antibody (PA1-46147), DNA PKcs Antibody (MA5-13404), versene solution (15040066) were purchased from Thermo scientific, United States.

    Radio Immunoprecipitation:

    Article Title: N-(2-Aminoethyl) Ethanolamine-Induced Morphological, Biochemical, and Biophysical Alterations in Vascular Matrix Associated With Dissecting Aortic Aneurysm
    Article Snippet: RIPA buffer (Radioimmunoprecipitation assay buffer) ( ; ) contains 25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, and 0.1% SDS. .. Trizma base (T-8524), sodium deoxycholate (D6750), sodium phosphate dibasic (S0876), sodium phosphate mono basic (S0751), urea (U5128), MTT (M2128), Tween 20 (P9416), and albumin bovine serum (BSA, A9647) were purchased from Sigma.

    Concentration Assay:

    Article Title: METTL3 and ALKBH5 oppositely regulate m6A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes
    Article Snippet: TBST (0.1% Tween 20 [Sigma-Aldrich, P9416] plus TBS [50 mM Tris-Cl, pH 7.5, 150 mM NaCl]) buffer including 1% non-fat milk was used to block the membranes and then was incubated overnight at 4°C using the indicated antibodies. .. TBST (0.1% Tween 20 [Sigma-Aldrich, P9416] plus TBS [50 mM Tris-Cl, pH 7.5, 150 mM NaCl]) buffer including 1% non-fat milk was used to block the membranes and then was incubated overnight at 4°C using the indicated antibodies.

    Article Title: Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption
    Article Snippet: After 10-cycle of sonication (Bioruptor Pico Sonication System, Diagenode, Denville, NJ, Category Number B01060001), proteins were extracted, and the concentration was determined by a BCA kit (Pierce, Rockford, IL; 23228). .. Equal amounts of 50 μg protein were resolved in SDS-PAGE, then transferred to PVDF membrane (ThermoFisher Scientific, Waltham, MA, Product number 88520) and blocked with 5% milk in PBST (0.1% Tween 20, Sigma-Aldrich, St. Louis, MO, P1379-500ml) for 1 hour at room temperature.

    Enzyme-linked Immunospot:

    Article Title: Tolerogenic Nanoparticles Induce Antigen-Specific Regulatory T Cells and Provide Therapeutic Efficacy and Transferrable Tolerance against Experimental Autoimmune Encephalomyelitis
    Article Snippet: Paragraph title: IFNγ ELISpot ... The plates were washed three times with wash buffer (WB) [PBS containing 0.05% Tween20 (Sigma P1379)] after which the plates were incubated for 90 min at room temperature with 2µg/ml biotinylated anti-mouse IFNγ detection antibody (BD Pharmingen 551881) diluted in DB.

    Staining:

    Article Title: Microporous methacrylated glycol chitosan-montmorillonite nanocomposite hydrogel for bone tissue engineering
    Article Snippet: MMT (682659-500G), glycidyl methacrylate (GMA, 151238-100G), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC, E1769-10G), tween-20 (p1379-500ML), p -nitrophenyl phosphate (N7653-100ML), β-glycerophosphate (G5422-100G), L -ascorbic acid (A5960-25G), dexamethasone (D4902-25MG), Nitro Blue Tetrazolium (NBT, N5514-25TAB), 5-bromo-4-chloro-3-indoxylphosphate (BCIP, B6149-50MG), alizarin red S (A5533), and ethylenediaminetetraacetic acid (EDTA, EDS-1KG) were supplied by Sigma-Aldrich (St. Louis, MO, USA). .. The live/dead staining solution (L3224) and cDNA transcription kit (18080-051) were purchased from Invitrogen (Carlsbad, CA, USA).

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  • 88
    Millipore goat anti sod1
    Immunohistochemistry for superoxide dismutase <t>(SOD1)</t> (a-c), manganese superoxide dismutase (SOD2) (d-f), catalase (CAT) (g-i) and glutathione peroxidase (GPx) (j-l) in the rat liver of the normal-(left column), ascorbic acid - (middle column) and Oenanthe javanica extract (OJE) - (right column) groups. Strong SOD1, SOD2, CAT, and GPx immunoreactive cells (arrows) are markedly increased in the OJE-group. Scale bar = 120 μ m.
    Goat Anti Sod1, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti sod1/product/Millipore
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    99
    Millipore glut1
    Myocardial glucose and lipid metabolism in MHC-D2 mice. Basal and insulin-stimulated glucose uptake in heart was measured following iv injection of 2-[ 14 C]DG in MHC-D2 mice and WT littermates. (A) Insulin-stimulated glucose uptake in heart (n = 4 for each group). (B) Basal glucose uptake in heart (n = 7 for each group). (C) Myocardial triglyceride levels (n = 4–6). (D) <t>GLUT1</t> expression in heart samples was measured using Western blot. (E) Systemic clearance of [ 3 H]-palmitate following a bolus injection (n = 4 for each group). (F) 3 H-glucose conversion into myocardial triglyceride (n = 4–6). (G) Basal glucose uptake in white adipose tissue (epididymal) (n = 3 for each group). (H) Intrahepatic triglyceride levels (n = 4–6). Data are presented as means ± SE. *, P
    Glut1, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore anti ngfr p75 polyclonal antibody
    <t>NGFR-p75,</t> TrkA and NGF protein levels in hippocampus (HPP). Mice were treated as in Fig. 4 . NGFR-p75 (** p
    Anti Ngfr P75 Polyclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunohistochemistry for superoxide dismutase (SOD1) (a-c), manganese superoxide dismutase (SOD2) (d-f), catalase (CAT) (g-i) and glutathione peroxidase (GPx) (j-l) in the rat liver of the normal-(left column), ascorbic acid - (middle column) and Oenanthe javanica extract (OJE) - (right column) groups. Strong SOD1, SOD2, CAT, and GPx immunoreactive cells (arrows) are markedly increased in the OJE-group. Scale bar = 120 μ m.

    Journal: Chinese Medical Journal

    Article Title: Effect of Oenanthe Javanica Extract on Antioxidant Enzyme in the Rat Liver

    doi: 10.4103/0366-6999.158363

    Figure Lengend Snippet: Immunohistochemistry for superoxide dismutase (SOD1) (a-c), manganese superoxide dismutase (SOD2) (d-f), catalase (CAT) (g-i) and glutathione peroxidase (GPx) (j-l) in the rat liver of the normal-(left column), ascorbic acid - (middle column) and Oenanthe javanica extract (OJE) - (right column) groups. Strong SOD1, SOD2, CAT, and GPx immunoreactive cells (arrows) are markedly increased in the OJE-group. Scale bar = 120 μ m.

    Article Snippet: To reduce background staining, the membranes were incubated with 5% nonfat dry milk in PBS containing 0.1% Tween 20 for 45 min, followed by incubation with goat anti-SOD1 (diluted 1:1000, Calbiochem, Darmstadt, Germany), goat anti-SOD2 (diluted 1:1000, Calbiochem), rabbit anti-CAT (diluted 1:1000, LabFrontier, Seoul, Korea) and sheep anti-GPx (diluted 1:1000, Chemicon International, Billerica, MA), peroxidase-conjugated donkey anti-goat IgG, goat anti-rabbit IgG or goat anti-sheep IgG (Santa Cruz, USA), and an ECL kit (Pierce Chemical, Rockford, USA).

    Techniques: Immunohistochemistry

    Myocardial glucose and lipid metabolism in MHC-D2 mice. Basal and insulin-stimulated glucose uptake in heart was measured following iv injection of 2-[ 14 C]DG in MHC-D2 mice and WT littermates. (A) Insulin-stimulated glucose uptake in heart (n = 4 for each group). (B) Basal glucose uptake in heart (n = 7 for each group). (C) Myocardial triglyceride levels (n = 4–6). (D) GLUT1 expression in heart samples was measured using Western blot. (E) Systemic clearance of [ 3 H]-palmitate following a bolus injection (n = 4 for each group). (F) 3 H-glucose conversion into myocardial triglyceride (n = 4–6). (G) Basal glucose uptake in white adipose tissue (epididymal) (n = 3 for each group). (H) Intrahepatic triglyceride levels (n = 4–6). Data are presented as means ± SE. *, P

    Journal: Endocrinology

    Article Title: Cardiac Expression of Human Type 2 Iodothyronine Deiodinase Increases Glucose Metabolism and Protects Against Doxorubicin-induced Cardiac Dysfunction in Male Mice

    doi: 10.1210/en.2012-2261

    Figure Lengend Snippet: Myocardial glucose and lipid metabolism in MHC-D2 mice. Basal and insulin-stimulated glucose uptake in heart was measured following iv injection of 2-[ 14 C]DG in MHC-D2 mice and WT littermates. (A) Insulin-stimulated glucose uptake in heart (n = 4 for each group). (B) Basal glucose uptake in heart (n = 7 for each group). (C) Myocardial triglyceride levels (n = 4–6). (D) GLUT1 expression in heart samples was measured using Western blot. (E) Systemic clearance of [ 3 H]-palmitate following a bolus injection (n = 4 for each group). (F) 3 H-glucose conversion into myocardial triglyceride (n = 4–6). (G) Basal glucose uptake in white adipose tissue (epididymal) (n = 3 for each group). (H) Intrahepatic triglyceride levels (n = 4–6). Data are presented as means ± SE. *, P

    Article Snippet: Membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 for 1 hour at room temperature and incubated with GLUT1 (Millipore, Billerica, Massachusetts), GLUT4 (Cell Signaling, Danvers, Massachusetts), Thr172 -phospho-AMPK (Cell Signaling), AMPK (Cell Signaling), Ser473 -phospho-Akt (Cell Signaling), or Akt (Cell Signaling) antibodies in Tris-buffered saline with Tween 20 containing 5% skim milk or BSA for overnight at 4°C.

    Techniques: Mouse Assay, IV Injection, Expressing, Western Blot, Injection

    Metabolic signaling pathways following DOX treatment in MHC-D2 and WT mice. Metabolic signaling pathways were examined using Western blot analysis in heart samples obtained at baseline and following DOX treatment in MHC-D2 and WT mice (n = 3–4 for each group). (A) Thr 172 -phosphorylation of AMPK and GLUT4 protein levels in heart of WT and MHC-D2 mice at baseline and after DOX treatment. (B) GLUT1 protein levels in heart of MHC-D2 mice at baseline and after DOX treatment. (C) Total Akt protein levels in heart of WT mice at baseline and after DOX treatment. (D) Ser 473 -phosphorylation of AKT in heart of WT mice. (E) Ratio of Ser 473 -phosphorylation of AKT and total Akt protein levels in WT mice. Data are presented as means ± SE. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading controls for all data. *, P

    Journal: Endocrinology

    Article Title: Cardiac Expression of Human Type 2 Iodothyronine Deiodinase Increases Glucose Metabolism and Protects Against Doxorubicin-induced Cardiac Dysfunction in Male Mice

    doi: 10.1210/en.2012-2261

    Figure Lengend Snippet: Metabolic signaling pathways following DOX treatment in MHC-D2 and WT mice. Metabolic signaling pathways were examined using Western blot analysis in heart samples obtained at baseline and following DOX treatment in MHC-D2 and WT mice (n = 3–4 for each group). (A) Thr 172 -phosphorylation of AMPK and GLUT4 protein levels in heart of WT and MHC-D2 mice at baseline and after DOX treatment. (B) GLUT1 protein levels in heart of MHC-D2 mice at baseline and after DOX treatment. (C) Total Akt protein levels in heart of WT mice at baseline and after DOX treatment. (D) Ser 473 -phosphorylation of AKT in heart of WT mice. (E) Ratio of Ser 473 -phosphorylation of AKT and total Akt protein levels in WT mice. Data are presented as means ± SE. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading controls for all data. *, P

    Article Snippet: Membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 for 1 hour at room temperature and incubated with GLUT1 (Millipore, Billerica, Massachusetts), GLUT4 (Cell Signaling, Danvers, Massachusetts), Thr172 -phospho-AMPK (Cell Signaling), AMPK (Cell Signaling), Ser473 -phospho-Akt (Cell Signaling), or Akt (Cell Signaling) antibodies in Tris-buffered saline with Tween 20 containing 5% skim milk or BSA for overnight at 4°C.

    Techniques: Mouse Assay, Western Blot

    NGFR-p75, TrkA and NGF protein levels in hippocampus (HPP). Mice were treated as in Fig. 4 . NGFR-p75 (** p

    Journal: PLoS ONE

    Article Title: BMP9 Protects Septal Neurons from Axotomy-Evoked Loss of Cholinergic Phenotype

    doi: 10.1371/journal.pone.0021166

    Figure Lengend Snippet: NGFR-p75, TrkA and NGF protein levels in hippocampus (HPP). Mice were treated as in Fig. 4 . NGFR-p75 (** p

    Article Snippet: After transfer of protein to an Immoblon P membrane (Millipore), the membrane was blocked with 5% nonfat dry milk in 1× Tris-buffered saline (TBS) containing 0.1% Tween 20 for 2 h and then probed overnight with either anti-NGFR-p75 polyclonal antibody (1∶1000) (Adavanced Targeting Systems); anti-CHAT polyclonal antibody (1∶1000) (Millipore); anti-CHT polyclonal antibody (1∶1000) (Millipore); anti-TrkA polyclonal antibody (1∶1000) (Millipore) or anti-β-actin monoclonal A5441 antibody (1∶5000) (Sigma).

    Techniques: Mouse Assay