tween 80  (Millipore)


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    Name:
    TWEEN 20
    Description:

    Catalog Number:
    p5927
    Price:
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    Applications:
    Non-ionic detergent
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    Structured Review

    Millipore tween 80
    TWEEN 20

    https://www.bioz.com/result/tween 80/product/Millipore
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tween 80 - by Bioz Stars, 2020-01
    83/100 stars

    Images

    1) Product Images from "Herbicidal Activity of Flavokawains and Related trans-Chalcones against Amaranthus tricolor L. and Echinochloacrus-galli (L.) Beauv."

    Article Title: Herbicidal Activity of Flavokawains and Related trans-Chalcones against Amaranthus tricolor L. and Echinochloacrus-galli (L.) Beauv.

    Journal: ACS Omega

    doi: 10.1021/acsomega.9b03144

    Inhibitory effects of 3 flavokawains and 42 synthetic chalcones on seed germination and shoot and root growth of barnyardgrass. Aqueous solutions of Tween 80 and butachlor were used as negative and positive controls, respectively. Different letters in each graph indicate significant differences ( p
    Figure Legend Snippet: Inhibitory effects of 3 flavokawains and 42 synthetic chalcones on seed germination and shoot and root growth of barnyardgrass. Aqueous solutions of Tween 80 and butachlor were used as negative and positive controls, respectively. Different letters in each graph indicate significant differences ( p

    Techniques Used:

    Inhibitory effects of 3 flavokawains and 18 related chalcones on seed germination (A) and shoot (B) and root (C) growth of Chinese amaranth. Aqueous solutions of Tween 80 and butachlor were used as negative and positive references, respectively. Different letters in each graph indicate significant differences ( p
    Figure Legend Snippet: Inhibitory effects of 3 flavokawains and 18 related chalcones on seed germination (A) and shoot (B) and root (C) growth of Chinese amaranth. Aqueous solutions of Tween 80 and butachlor were used as negative and positive references, respectively. Different letters in each graph indicate significant differences ( p

    Techniques Used:

    Inhibitory effects of 24 synthetic chalcones on seed germination (A) and shoot (B) and root (C) growth of Chinese amaranth. Aqueous solutions of Tween 80 and butachlor were used as negative and positive controls, respectively. Different letters in each graph indicate significant differences ( p
    Figure Legend Snippet: Inhibitory effects of 24 synthetic chalcones on seed germination (A) and shoot (B) and root (C) growth of Chinese amaranth. Aqueous solutions of Tween 80 and butachlor were used as negative and positive controls, respectively. Different letters in each graph indicate significant differences ( p

    Techniques Used:

    Inhibitory effects of chalcone 14f on seed germination, shoot, and root growths of (A) barnyardgrass and (B) Chinese amaranth. Aqueous solutions of Tween 80 were used as negative control.
    Figure Legend Snippet: Inhibitory effects of chalcone 14f on seed germination, shoot, and root growths of (A) barnyardgrass and (B) Chinese amaranth. Aqueous solutions of Tween 80 were used as negative control.

    Techniques Used: Negative Control

    2) Product Images from "Mexican oregano (Lippia graveolens) essential oil-in-water emulsions: impact of emulsifier type on the antifungal activity of Candida albicans"

    Article Title: Mexican oregano (Lippia graveolens) essential oil-in-water emulsions: impact of emulsifier type on the antifungal activity of Candida albicans

    Journal: Food Science and Biotechnology

    doi: 10.1007/s10068-018-0499-6

    Particle size trend of emulsions storage at three temperatures (25 and 40 °C): A Tween 80 (Blue bars), B Hydroxylated soy lecithin (gray bars), and C Gum arabic (Red bars). (Color figure online)
    Figure Legend Snippet: Particle size trend of emulsions storage at three temperatures (25 and 40 °C): A Tween 80 (Blue bars), B Hydroxylated soy lecithin (gray bars), and C Gum arabic (Red bars). (Color figure online)

    Techniques Used:

    Antifungal activity of major monoterpenes concentration (thymol and carvacrol) against  C. albicans .  A  Tween 80,  B  Hydroxylated soy lecithin, and  C  Gum arabic. Columns having common letters are not significantly different ( p   >  0.05)
    Figure Legend Snippet: Antifungal activity of major monoterpenes concentration (thymol and carvacrol) against C. albicans . A Tween 80, B Hydroxylated soy lecithin, and C Gum arabic. Columns having common letters are not significantly different ( p   >  0.05)

    Techniques Used: Activity Assay, Concentration Assay

    Thymol and carvacrol concentration contained in the emulsions:  A  T80 (Tween 80),  B  HSL (Hydroxylated soy lecithin), and  C  GA (Gum arabic)
    Figure Legend Snippet: Thymol and carvacrol concentration contained in the emulsions: A T80 (Tween 80), B HSL (Hydroxylated soy lecithin), and C GA (Gum arabic)

    Techniques Used: Concentration Assay

    3) Product Images from "Mexican oregano (Lippia graveolens) essential oil-in-water emulsions: impact of emulsifier type on the antifungal activity of Candida albicans"

    Article Title: Mexican oregano (Lippia graveolens) essential oil-in-water emulsions: impact of emulsifier type on the antifungal activity of Candida albicans

    Journal: Food Science and Biotechnology

    doi: 10.1007/s10068-018-0499-6

    Particle size trend of emulsions storage at three temperatures (25 and 40 °C): A Tween 80 (Blue bars), B Hydroxylated soy lecithin (gray bars), and C Gum arabic (Red bars). (Color figure online)
    Figure Legend Snippet: Particle size trend of emulsions storage at three temperatures (25 and 40 °C): A Tween 80 (Blue bars), B Hydroxylated soy lecithin (gray bars), and C Gum arabic (Red bars). (Color figure online)

    Techniques Used:

    Thymol and carvacrol concentration contained in the emulsions:  A  T80 (Tween 80),  B  HSL (Hydroxylated soy lecithin), and  C  GA (Gum arabic)
    Figure Legend Snippet: Thymol and carvacrol concentration contained in the emulsions: A T80 (Tween 80), B HSL (Hydroxylated soy lecithin), and C GA (Gum arabic)

    Techniques Used: Concentration Assay

    Antifungal activity of major monoterpenes concentration (thymol and carvacrol) against  C. albicans .  A  Tween 80,  B  Hydroxylated soy lecithin, and  C  Gum arabic. Columns having common letters are not significantly different ( p >  0.05)
    Figure Legend Snippet: Antifungal activity of major monoterpenes concentration (thymol and carvacrol) against C. albicans . A Tween 80, B Hydroxylated soy lecithin, and C Gum arabic. Columns having common letters are not significantly different ( p >  0.05)

    Techniques Used: Activity Assay, Concentration Assay

    4) Product Images from "Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate"

    Article Title: Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate

    Journal: Microorganisms

    doi: 10.3390/microorganisms7110578

    Growth curves of yeast strains cultivated in different media for 96 h. Biomass production in g/L was measured in the following media: ( a ) Medium with glucose; ( b ) medium with glycerol; ( c ) medium with crude animal fat; ( d ) medium with emulsified fat by Tween 80; ( e ) medium with enzymatically hydrolyzed fat; and ( f ) medium with animal fat and the addition of lipase. Abbreviations: CCY (Culture Collection of Yeasts), RG ( Rhodotorula glutinis  CCY 20-2-26), CM ( Cystofilobasidium macerans  CCY 10-1-2), RM ( Rhodotorula mucilaginosa  CCY 19-4-6), and SP ( Sporobolomyces pararoseus  CCY 19-9-6).
    Figure Legend Snippet: Growth curves of yeast strains cultivated in different media for 96 h. Biomass production in g/L was measured in the following media: ( a ) Medium with glucose; ( b ) medium with glycerol; ( c ) medium with crude animal fat; ( d ) medium with emulsified fat by Tween 80; ( e ) medium with enzymatically hydrolyzed fat; and ( f ) medium with animal fat and the addition of lipase. Abbreviations: CCY (Culture Collection of Yeasts), RG ( Rhodotorula glutinis CCY 20-2-26), CM ( Cystofilobasidium macerans CCY 10-1-2), RM ( Rhodotorula mucilaginosa CCY 19-4-6), and SP ( Sporobolomyces pararoseus CCY 19-9-6).

    Techniques Used:

    Changes of pH of individual media during yeast cultivation. Changes of pH were recorded in following media: ( a ) Medium with glucose; ( b ) Medium with glycerol; ( c ) Medium with crude animal fat; ( d ) Medium with emulsified fat by Tween 80; ( e ) Medium with enzymatically hydrolyzed fat; ( f ) Medium with animal fat an addition of lipase. Abbreviations: RG ( Rhodotorula glutinis  CCY 20-2-26), CM ( Cystofilobasidium macerans  CCY 10-1-2), RM ( Rhodotorula mucilaginosa  CCY 19-4-6) and SP ( Sporobolomyces pararoseus  CCY 19-9-6).
    Figure Legend Snippet: Changes of pH of individual media during yeast cultivation. Changes of pH were recorded in following media: ( a ) Medium with glucose; ( b ) Medium with glycerol; ( c ) Medium with crude animal fat; ( d ) Medium with emulsified fat by Tween 80; ( e ) Medium with enzymatically hydrolyzed fat; ( f ) Medium with animal fat an addition of lipase. Abbreviations: RG ( Rhodotorula glutinis CCY 20-2-26), CM ( Cystofilobasidium macerans CCY 10-1-2), RM ( Rhodotorula mucilaginosa CCY 19-4-6) and SP ( Sporobolomyces pararoseus CCY 19-9-6).

    Techniques Used:

    5) Product Images from "Antimicrobial Electrospun Polycaprolactone-Based Wound Dressings: An In Vitro Study About the Importance of the Direct Contact to Elicit Bactericidal Activity"

    Article Title: Antimicrobial Electrospun Polycaprolactone-Based Wound Dressings: An In Vitro Study About the Importance of the Direct Contact to Elicit Bactericidal Activity

    Journal: Advances in Wound Care

    doi: 10.1089/wound.2018.0893

    Drug release kinetics obtained by flushing through a fixed bed composed of the electrospun mats of PBS (with Tween 80, 2% w/v) at 37°C with a flow rate of 1 mL/min (data shown correspond with mean and standard deviation of three independent experiments for each material). PBS, phosphate-buffered saline. Color images are available online.
    Figure Legend Snippet: Drug release kinetics obtained by flushing through a fixed bed composed of the electrospun mats of PBS (with Tween 80, 2% w/v) at 37°C with a flow rate of 1 mL/min (data shown correspond with mean and standard deviation of three independent experiments for each material). PBS, phosphate-buffered saline. Color images are available online.

    Techniques Used: Flow Cytometry, Standard Deviation

    6) Product Images from "Antimicrobial Electrospun Polycaprolactone-Based Wound Dressings: An In Vitro Study About the Importance of the Direct Contact to Elicit Bactericidal Activity"

    Article Title: Antimicrobial Electrospun Polycaprolactone-Based Wound Dressings: An In Vitro Study About the Importance of the Direct Contact to Elicit Bactericidal Activity

    Journal: Advances in Wound Care

    doi: 10.1089/wound.2018.0893

    Drug release kinetics obtained by flushing through a fixed bed composed of the electrospun mats of PBS (with Tween 80, 2% w/v) at 37°C with a flow rate of 1 mL/min (data shown correspond with mean and standard deviation of three independent experiments for each material). PBS, phosphate-buffered saline. Color images are available online.
    Figure Legend Snippet: Drug release kinetics obtained by flushing through a fixed bed composed of the electrospun mats of PBS (with Tween 80, 2% w/v) at 37°C with a flow rate of 1 mL/min (data shown correspond with mean and standard deviation of three independent experiments for each material). PBS, phosphate-buffered saline. Color images are available online.

    Techniques Used: Flow Cytometry, Standard Deviation

    7) Product Images from "Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes"

    Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2018.09.025

    Isothermal Titration Calorimetry experiments for (a) βSBECD (concentration: 60.0 mM) and (b) HPβCD (concentration: 60.0 mM) in LGK974 (concentration: 3.0 mM) (square) and nonlinear fitting by Wiseman Isotherm (line). (c) Thermodynamic parameters of CD-LGK974 interactions determined by isothermal titration calorimetry experiments (average stoichiometry N of the complex in solution, enthalpy ΔH°, entropy TΔS°, equilibrium constant K eq ). (d) Free LGK974, LGK974 suspension in CMC/Tween 80 and CD:LGK974 solubility in PBS.
    Figure Legend Snippet: Isothermal Titration Calorimetry experiments for (a) βSBECD (concentration: 60.0 mM) and (b) HPβCD (concentration: 60.0 mM) in LGK974 (concentration: 3.0 mM) (square) and nonlinear fitting by Wiseman Isotherm (line). (c) Thermodynamic parameters of CD-LGK974 interactions determined by isothermal titration calorimetry experiments (average stoichiometry N of the complex in solution, enthalpy ΔH°, entropy TΔS°, equilibrium constant K eq ). (d) Free LGK974, LGK974 suspension in CMC/Tween 80 and CD:LGK974 solubility in PBS.

    Techniques Used: Isothermal Titration Calorimetry, Concentration Assay, Solubility

    8) Product Images from "Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes"

    Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2018.09.025

    Isothermal Titration Calorimetry experiments for (a) βSBECD (concentration: 60.0 mM) and (b) HPβCD (concentration: 60.0 mM) in LGK974 (concentration: 3.0 mM) (square) and nonlinear fitting by Wiseman Isotherm (line). (c) Thermodynamic parameters of CD-LGK974 interactions determined by isothermal titration calorimetry experiments (average stoichiometry N of the complex in solution, enthalpy ΔH°, entropy TΔS°, equilibrium constant K eq ). (d) Free LGK974, LGK974 suspension in CMC/Tween 80 and CD:LGK974 solubility in PBS.
    Figure Legend Snippet: Isothermal Titration Calorimetry experiments for (a) βSBECD (concentration: 60.0 mM) and (b) HPβCD (concentration: 60.0 mM) in LGK974 (concentration: 3.0 mM) (square) and nonlinear fitting by Wiseman Isotherm (line). (c) Thermodynamic parameters of CD-LGK974 interactions determined by isothermal titration calorimetry experiments (average stoichiometry N of the complex in solution, enthalpy ΔH°, entropy TΔS°, equilibrium constant K eq ). (d) Free LGK974, LGK974 suspension in CMC/Tween 80 and CD:LGK974 solubility in PBS.

    Techniques Used: Isothermal Titration Calorimetry, Concentration Assay, Solubility

    9) Product Images from "DMTS is an effective treatment in both an inhalation and injection model for cyanide poisoning using unanesthetized mice"

    Article Title: DMTS is an effective treatment in both an inhalation and injection model for cyanide poisoning using unanesthetized mice

    Journal: Clinical toxicology (Philadelphia, Pa.)

    doi: 10.1080/15563650.2017.1376749

    Dose–response curves of subcutaneous KCN in the presence of vehicle control (25% Span80, 75% Tween 80) or DMTS at 50 mg/kg (0.398 mmol/kg), 100 mg/kg (0.795 mmol/kg), 200 mg/kg (1.590 mmol/kg), and 300 mg/kg (2.39 mmol/kg) ( n  = 30–36 male
    Figure Legend Snippet: Dose–response curves of subcutaneous KCN in the presence of vehicle control (25% Span80, 75% Tween 80) or DMTS at 50 mg/kg (0.398 mmol/kg), 100 mg/kg (0.795 mmol/kg), 200 mg/kg (1.590 mmol/kg), and 300 mg/kg (2.39 mmol/kg) ( n = 30–36 male

    Techniques Used:

    10) Product Images from "Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes"

    Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2018.09.025

    Isothermal Titration Calorimetry experiments for (a) βSBECD (concentration: 60.0 mM) and (b) HPβCD (concentration: 60.0 mM) in LGK974 (concentration: 3.0 mM) (square) and nonlinear fitting by Wiseman Isotherm (line). (c) Thermodynamic parameters of CD-LGK974 interactions determined by isothermal titration calorimetry experiments (average stoichiometry N of the complex in solution, enthalpy ΔH°, entropy TΔS°, equilibrium constant K eq ). (d) Free LGK974, LGK974 suspension in CMC/Tween 80 and CD:LGK974 solubility in PBS.
    Figure Legend Snippet: Isothermal Titration Calorimetry experiments for (a) βSBECD (concentration: 60.0 mM) and (b) HPβCD (concentration: 60.0 mM) in LGK974 (concentration: 3.0 mM) (square) and nonlinear fitting by Wiseman Isotherm (line). (c) Thermodynamic parameters of CD-LGK974 interactions determined by isothermal titration calorimetry experiments (average stoichiometry N of the complex in solution, enthalpy ΔH°, entropy TΔS°, equilibrium constant K eq ). (d) Free LGK974, LGK974 suspension in CMC/Tween 80 and CD:LGK974 solubility in PBS.

    Techniques Used: Isothermal Titration Calorimetry, Concentration Assay, Solubility

    11) Product Images from "Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes"

    Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2018.09.025

    Isothermal Titration Calorimetry experiments for (a) βSBECD (concentration: 60.0 mM) and (b) HPβCD (concentration: 60.0 mM) in LGK974 (concentration: 3.0 mM) (square) and nonlinear fitting by Wiseman Isotherm (line). (c) Thermodynamic parameters of CD-LGK974 interactions determined by isothermal titration calorimetry experiments (average stoichiometry N of the complex in solution, enthalpy ΔH°, entropy TΔS°, equilibrium constant K eq ). (d) Free LGK974, LGK974 suspension in CMC/Tween 80 and CD:LGK974 solubility in PBS.
    Figure Legend Snippet: Isothermal Titration Calorimetry experiments for (a) βSBECD (concentration: 60.0 mM) and (b) HPβCD (concentration: 60.0 mM) in LGK974 (concentration: 3.0 mM) (square) and nonlinear fitting by Wiseman Isotherm (line). (c) Thermodynamic parameters of CD-LGK974 interactions determined by isothermal titration calorimetry experiments (average stoichiometry N of the complex in solution, enthalpy ΔH°, entropy TΔS°, equilibrium constant K eq ). (d) Free LGK974, LGK974 suspension in CMC/Tween 80 and CD:LGK974 solubility in PBS.

    Techniques Used: Isothermal Titration Calorimetry, Concentration Assay, Solubility

    12) Product Images from "A Novel Nanoparticle Formulation for Sustained Paclitaxel Delivery"

    Article Title: A Novel Nanoparticle Formulation for Sustained Paclitaxel Delivery

    Journal:

    doi: 10.1208/s12249-008-9063-7

    The in vitro release profiles of PTX ( closed diamonds ) or DEX ( closed squares ) from chitosan/GMO nanoparticles into a release medium containing: a PBS without surfactant and b PBS with surfactant (0.02% [ v / v ] Tween-80)
    Figure Legend Snippet: The in vitro release profiles of PTX ( closed diamonds ) or DEX ( closed squares ) from chitosan/GMO nanoparticles into a release medium containing: a PBS without surfactant and b PBS with surfactant (0.02% [ v / v ] Tween-80)

    Techniques Used: In Vitro

    Related Articles

    High Performance Liquid Chromatography:

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    Article Snippet: Vancomycin (catalog no. 861987-250MG, lot 087K0694), ciprofloxacin (catalog no. 17850-25G-F, lot 0001396108), oxacillin (catalog no. O1002-1G, lot 018K0610), penicillin G (catalog no. 13752-1G-F, lot WE376306/1), trimethoprim (catalog no. T7883-5G, lot 078K1522), colistin (catalog no. C4461-1G, lot 036K1374, 15,000 units per mg), polymyxin B (catalog no. P0972-10MU, lot 453306, ≥6,000 USP units per mg), rifampin (catalog no. R3501-250MG), polysorbate 80 (Tween 80, catalog no. P8074-500ml), and human serum (catalog no. H4522-20ml) were obtained from Sigma-Aldrich (Sydney, NSW, Australia). .. MCC223, MCC310, and MCC520 were synthesized from vancomycin and purified by high-performance liquid chromatography (HPLC) to > 95% purity ( ).

    Article Title: Evaporative Light Scattering Detection Based HPLC Method for the Determination of Polysorbate 80 in Therapeutic Protein Formulations
    Article Snippet: Polysorbate 80 used in this study was purchased from Sigma Aldrich (St. Louis, MO). .. Acetonitrile (HPLC grade) was purchased from EM Science (Gibbstown, NJ).

    Recombinant:

    Article Title: A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification
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    Filtration:

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    In Vitro:

    Article Title: Translocation of Crohn's disease Escherichia coli across M-cells: contrasting effects of soluble plant fibres and emulsifiers
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    Synthesized:

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    Article Title: Enhancement of encapsulation efficiency of nanoemulsion-containing aripiprazole for the treatment of schizophrenia using mixture experimental design
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    Transmission Assay:

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    Incubation:

    Article Title: A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification
    Article Snippet: In brief, 200 microliters of blocking solutions were placed per well: (a) PBS (no blocking solution), (b) BSA-based blocking solution: 0.25% BSA solution in 15 mM borate buffer containing 0.05% polysorbate 20 and 100 mM NaCl, pH 8.5, (c) Superblock (TBS) (Thermo Fisher Scientific #37515) containing 0.1% polysorbate 20 (Superblock-T), (d) 1% polysorbate 20 in PBS, (e) 1%Triton X-100 in PBS, (f) 5% skim milk in PBS containing 0,1% polysorbate 20 and (g) 8 nM Octyl b-D-glucopyranoside (Sigma-Aldrich #O8001) in PBS containing 1.17% BSA. .. These blocking solutions were incubated for 16 h at RT, and then washed before proceeding with the ELISA assay with either the apoE4 specific monoclonal antibody 4E4, or the polyclonal pan-apoE antibody as above described.

    Blocking Assay:

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    Article Snippet: .. In brief, 200 microliters of blocking solutions were placed per well: (a) PBS (no blocking solution), (b) BSA-based blocking solution: 0.25% BSA solution in 15 mM borate buffer containing 0.05% polysorbate 20 and 100 mM NaCl, pH 8.5, (c) Superblock (TBS) (Thermo Fisher Scientific #37515) containing 0.1% polysorbate 20 (Superblock-T), (d) 1% polysorbate 20 in PBS, (e) 1%Triton X-100 in PBS, (f) 5% skim milk in PBS containing 0,1% polysorbate 20 and (g) 8 nM Octyl b-D-glucopyranoside (Sigma-Aldrich #O8001) in PBS containing 1.17% BSA. .. These blocking solutions were incubated for 16 h at RT, and then washed before proceeding with the ELISA assay with either the apoE4 specific monoclonal antibody 4E4, or the polyclonal pan-apoE antibody as above described.

    Sampling:

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    Enzyme-linked Immunosorbent Assay:

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    Concentration Assay:

    Article Title: Nanoparticle-Formulated Curcumin Prevents Posttherapeutic Disease Reactivation and Reinfection with Mycobacterium tuberculosis following Isoniazid Therapy
    Article Snippet: This solution was then stirred in a high-speed homogenizer (T 25 digital ULTRA-TURAX, IKA, USA) at 12,000–15,000 RPM and a required volume of Milli Q water containing 0.1% citric acid (Merck, India) was added to it slowly over a period of 30 min until the ethanol concentration became 40% (V/V) and curcumin nanoparticles started to precipitate from the solution. .. The aqueous suspension was then made to 0.1% polysorbate 80 (Sigma, USA) and homogenized at 12,000–15,000 RPM (T 25 digital ULTRA-TURRAX, IKA, USA) again for 1 h and filtered.

    Purification:

    Article Title: Enhanced brain targeting of temozolomide in polysorbate-80 coated polybutylcyanoacrylate nanoparticles
    Article Snippet: Polysorbate-80 was obtained from Sigma Chemical Co (St. Louis, MO). .. All other materials and reagents used were of analytical/high-pressure liquid chromatography grade and without further purification.

    Article Title: Effects of Microplate Type and Broth Additives on Microdilution MIC Susceptibility Assays
    Article Snippet: Vancomycin (catalog no. 861987-250MG, lot 087K0694), ciprofloxacin (catalog no. 17850-25G-F, lot 0001396108), oxacillin (catalog no. O1002-1G, lot 018K0610), penicillin G (catalog no. 13752-1G-F, lot WE376306/1), trimethoprim (catalog no. T7883-5G, lot 078K1522), colistin (catalog no. C4461-1G, lot 036K1374, 15,000 units per mg), polymyxin B (catalog no. P0972-10MU, lot 453306, ≥6,000 USP units per mg), rifampin (catalog no. R3501-250MG), polysorbate 80 (Tween 80, catalog no. P8074-500ml), and human serum (catalog no. H4522-20ml) were obtained from Sigma-Aldrich (Sydney, NSW, Australia). .. MCC223, MCC310, and MCC520 were synthesized from vancomycin and purified by high-performance liquid chromatography (HPLC) to > 95% purity ( ).

    Inhibition:

    Article Title: Translocation of Crohn's disease Escherichia coli across M-cells: contrasting effects of soluble plant fibres and emulsifiers
    Article Snippet: Concentrations were chosen based on preliminary studies of inhibition of bacterial haemagglutination, inhibition of attachment to, and invasion of intestinal cells, and were within the range of intraluminal concentrations that would be readily achievable with dietary supplementation. .. Common food emulsifiers used in the processed food industry, polysorbate-60 and polysorbate-80, were obtained from Sigma-Aldrich (Poole, UK).

    Microscopy:

    Article Title: Nanoparticle-Formulated Curcumin Prevents Posttherapeutic Disease Reactivation and Reinfection with Mycobacterium tuberculosis following Isoniazid Therapy
    Article Snippet: The aqueous suspension was then made to 0.1% polysorbate 80 (Sigma, USA) and homogenized at 12,000–15,000 RPM (T 25 digital ULTRA-TURRAX, IKA, USA) again for 1 h and filtered. .. Particle size was determined by a high resolution transmission electron microscope (JEM 2100F, JEOL, USA).

    Serial Time-encoded Amplified Microscopy:

    Article Title: Increased Effectiveness of Microbiological Verification by Concentration-Dependent Neutralization of Sanitizers Used in Poultry Slaughter and Fabrication Allowing Salmonella enterica Survival
    Article Snippet: Stock solutions of refined soy lecithin and polysorbate 80 (Sigma-Aldrich Co., St. Louis, MO, USA) were prepared in distilled water so as to obtain two times (2X) their standard concentrations in D/E buffer (soy lecithin: 7.0 g/L; polysorbate 80: 5.0 g/L) upon completion of final sample preparation. .. All solutions were then transferred into screw-cap bottles, subjected to steam sterilization (15 min, 121 °C), and tempered to room temperature prior to use.

    Sample Prep:

    Article Title: Increased Effectiveness of Microbiological Verification by Concentration-Dependent Neutralization of Sanitizers Used in Poultry Slaughter and Fabrication Allowing Salmonella enterica Survival
    Article Snippet: .. Stock solutions of refined soy lecithin and polysorbate 80 (Sigma-Aldrich Co., St. Louis, MO, USA) were prepared in distilled water so as to obtain two times (2X) their standard concentrations in D/E buffer (soy lecithin: 7.0 g/L; polysorbate 80: 5.0 g/L) upon completion of final sample preparation. ..

    Transformation Assay:

    Article Title: Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex
    Article Snippet: Mycobacteria, Growth, and Inoculum Preparation M. bovis BCG lux (Montréal vaccine strain), transformed with the plasmid pSMT1 containing the luxAB genes of Vibrio harveyi, was kindly donated by Professor Young’s lab ( ). .. Mid-log phase cultures of BCG lux were prepared in Middlebrook 7H9 broth (Difco, US), supplemented with 0.2% glycerol (Sigma-Aldrich, UK), 0.05% polysorbate 80 (Sigma-Aldrich, UK) and 10% albumin dextrose catalase (ADC) as described previously ( ).

    Binding Assay:

    Article Title: A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification
    Article Snippet: To test the binding of apoE to blocked polystyrene ELISA plates, recombinant apoE4 or plasma from an APOE ε3/ε4 donor were allowed to interact with ELISA plate wells previously treated with different blocking solutions. .. In brief, 200 microliters of blocking solutions were placed per well: (a) PBS (no blocking solution), (b) BSA-based blocking solution: 0.25% BSA solution in 15 mM borate buffer containing 0.05% polysorbate 20 and 100 mM NaCl, pH 8.5, (c) Superblock (TBS) (Thermo Fisher Scientific #37515) containing 0.1% polysorbate 20 (Superblock-T), (d) 1% polysorbate 20 in PBS, (e) 1%Triton X-100 in PBS, (f) 5% skim milk in PBS containing 0,1% polysorbate 20 and (g) 8 nM Octyl b-D-glucopyranoside (Sigma-Aldrich #O8001) in PBS containing 1.17% BSA.

    Liquid Chromatography:

    Article Title: Enhanced brain targeting of temozolomide in polysorbate-80 coated polybutylcyanoacrylate nanoparticles
    Article Snippet: Polysorbate-80 was obtained from Sigma Chemical Co (St. Louis, MO). .. All other materials and reagents used were of analytical/high-pressure liquid chromatography grade and without further purification.

    Plasmid Preparation:

    Article Title: Galleria mellonella: An Infection Model for Screening Compounds Against the Mycobacterium tuberculosis Complex
    Article Snippet: Mycobacteria, Growth, and Inoculum Preparation M. bovis BCG lux (Montréal vaccine strain), transformed with the plasmid pSMT1 containing the luxAB genes of Vibrio harveyi, was kindly donated by Professor Young’s lab ( ). .. Mid-log phase cultures of BCG lux were prepared in Middlebrook 7H9 broth (Difco, US), supplemented with 0.2% glycerol (Sigma-Aldrich, UK), 0.05% polysorbate 80 (Sigma-Aldrich, UK) and 10% albumin dextrose catalase (ADC) as described previously ( ).

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