Structured Review

Millipore tween 20
Surviving populations (log CFU/sample or log CFU/250 ml) of  Escherichia coli  O157:H7,  Salmonella  Typhimurium, and  Listeria monocytogenes  on sample surfaces or in washing solution (tap water [TW] plus 0.1% Tween 20) treated with the Spindle in
Tween 20, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tween 20/product/Millipore
Average 99 stars, based on 418 article reviews
Price from $9.99 to $1999.99
tween 20 - by Bioz Stars, 2020-04
99/100 stars

Images

1) Product Images from "Decontamination Effect of the Spindle and 222-Nanometer Krypton-Chlorine Excimer Lamp Combination against Pathogens on Apples (Malus domestica Borkh.) and Bell Peppers (Capsicum annuum L.)"

Article Title: Decontamination Effect of the Spindle and 222-Nanometer Krypton-Chlorine Excimer Lamp Combination against Pathogens on Apples (Malus domestica Borkh.) and Bell Peppers (Capsicum annuum L.)

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.00006-19

Surviving populations (log CFU/sample or log CFU/250 ml) of  Escherichia coli  O157:H7,  Salmonella  Typhimurium, and  Listeria monocytogenes  on sample surfaces or in washing solution (tap water [TW] plus 0.1% Tween 20) treated with the Spindle in
Figure Legend Snippet: Surviving populations (log CFU/sample or log CFU/250 ml) of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on sample surfaces or in washing solution (tap water [TW] plus 0.1% Tween 20) treated with the Spindle in

Techniques Used:

Related Articles

Luciferase:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: Wnt signaling activity was quantified by normalizing the luciferase signal generated by Wnt activation to the tdTomato signal generated by the tumor burden. .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Construct:

Article Title: The T cell antigen receptor α transmembrane domain coordinates triggering through regulation of bilayer immersion and CD3 subunit associations
Article Snippet: In the SMSC assay, bond lifetime measurements were carried-out using tethers constructed from half anti-biotin antibody functionalized DNA to bridge the anti-digoxygenin–coated polystyrene beads (1.0 μm in diameter, Spherotech Inc.) at one end and biotin-labeled VSV8Kb at the other end. .. After washing with PBST buffer (1X PBS + 0.02% (v/v) Tween-20) two times, the bead slurry was diluted 200-fold with 5 mg/mL Bovine serum albumin (BSA) (Sigma-Aldrich) in colorless DMEM medium (Sigma-Aldrich) for bond lifetime measurements.

Enzyme-linked Immunosorbent Assay:

Article Title: Expression of Human Apolipoprotein E3 or E4 in the Brains ofApoe−/− Mice: Isoform-Specific Effects on Neurodegeneration
Article Snippet: After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions. .. After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions.

Incubation:

Article Title: mRNAs biotinylated within the 5′ cap and protected against decapping: new tools to capture RNA–protein complexes
Article Snippet: .. Membranes were washed three times with PBS supplemented with 0.2% Tween 20 and incubated for 1 h with horseradish-peroxidase-conjugated rat secondary antibodies (Sigma-Aldrich, St Louis, MO) diluted 1 : 10 000. .. Bound antibodies were detected by using the Western Lightning PlusECL reagent (Perkin-Elmer, Waltham, MA).

Article Title: Expression of Human Apolipoprotein E3 or E4 in the Brains ofApoe−/− Mice: Isoform-Specific Effects on Neurodegeneration
Article Snippet: Absorbance values for wells in which the incubation with anti-synaptophysin antibody was omitted were subtracted from the values obtained. .. After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions.

Article Title: The T cell antigen receptor α transmembrane domain coordinates triggering through regulation of bilayer immersion and CD3 subunit associations
Article Snippet: After washing with PBST buffer (1X PBS + 0.02% (v/v) Tween-20) two times, the bead slurry was diluted 200-fold with 5 mg/mL Bovine serum albumin (BSA) (Sigma-Aldrich) in colorless DMEM medium (Sigma-Aldrich) for bond lifetime measurements. .. 20 μL of the cell suspension was transferred into the flow chamber, and cells were bound to a coverslip, after 30 mins incubation at 37°C and 5% CO2 the coverslip surface was further blocked by 5 mg/mL BSA in colorless DMEM medium.

Activity Assay:

Article Title: Mexican oregano (Lippia graveolens) essential oil-in-water emulsions: impact of emulsifier type on the antifungal activity of Candida albicans
Article Snippet: Gum arabic (GA) Instant-gum was purchased from Nexira (Mexico City, Mexico), Tween 80 (T80) was purchased from Sigma-Aldrich (State of Mexico, Mexico). .. C. albicans (ATCC 10231) was used to evaluate the antifungal activity.

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: Wnt signaling activity was quantified by normalizing the luciferase signal generated by Wnt activation to the tdTomato signal generated by the tumor burden. .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Bradford Assay:

Article Title: Expression of Human Apolipoprotein E3 or E4 in the Brains ofApoe−/− Mice: Isoform-Specific Effects on Neurodegeneration
Article Snippet: After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions. .. After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions.

Transplantation Assay:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: One week following transplantation, mice were treated with 100 mg/kg D-Luciferin via I.P. administration (Perkin Elmer), and tumor burden was quantified by detecting tdTomato fluorescence using whole-animal imaging with an IVIS system (Perkin Elmer). .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Western Blot:

Article Title: mRNAs biotinylated within the 5′ cap and protected against decapping: new tools to capture RNA–protein complexes
Article Snippet: Membranes were washed three times with PBS supplemented with 0.2% Tween 20 and incubated for 1 h with horseradish-peroxidase-conjugated rat secondary antibodies (Sigma-Aldrich, St Louis, MO) diluted 1 : 10 000. .. Bound antibodies were detected by using the Western Lightning PlusECL reagent (Perkin-Elmer, Waltham, MA).

RNA Binding Assay:

Article Title: mRNAs biotinylated within the 5′ cap and protected against decapping: new tools to capture RNA–protein complexes
Article Snippet: In this case, it is essential to perform the two last washing steps in the absence of RNAsin, as the elution of RNA-binding proteins is achieved by addition of 1 µl RNase A/T1 cocktail (2 µg µl−1 RNase A; 5 U µl−1 RNase T1) in 50 mM triethylamonium bicarbonate (pH 8.5) and 5 mM EDTA. .. Membranes were washed three times with PBS supplemented with 0.2% Tween 20 and incubated for 1 h with horseradish-peroxidase-conjugated rat secondary antibodies (Sigma-Aldrich, St Louis, MO) diluted 1 : 10 000.

High Performance Liquid Chromatography:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: .. Hydrochloric acid 37% ACS reagent, carboxymethylcellulose sodium salt (CMC), Tween-80, HPLC grade acetonitrile, trifluoroacetic acid 99% reagent plus, and dimethyl sulfoxide 99.9% ACS reagent were all obtained from Sigma-Aldrich (Milwaukee, WI, USA). .. Phosphate buffered saline, pH 7.4 was obtained from ThermoFisher Scientific (Cambridge, MA, USA).

Flow Cytometry:

Article Title: The T cell antigen receptor α transmembrane domain coordinates triggering through regulation of bilayer immersion and CD3 subunit associations
Article Snippet: After washing with PBST buffer (1X PBS + 0.02% (v/v) Tween-20) two times, the bead slurry was diluted 200-fold with 5 mg/mL Bovine serum albumin (BSA) (Sigma-Aldrich) in colorless DMEM medium (Sigma-Aldrich) for bond lifetime measurements. .. 20 μL of the cell suspension was transferred into the flow chamber, and cells were bound to a coverslip, after 30 mins incubation at 37°C and 5% CO2 the coverslip surface was further blocked by 5 mg/mL BSA in colorless DMEM medium.

Generated:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: Wnt signaling activity was quantified by normalizing the luciferase signal generated by Wnt activation to the tdTomato signal generated by the tumor burden. .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Inhibition:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: Paragraph title: 2.12. Wnt signaling inhibition - in vivo luminescence/fluorescence ... Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Imaging:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: One week following transplantation, mice were treated with 100 mg/kg D-Luciferin via I.P. administration (Perkin Elmer), and tumor burden was quantified by detecting tdTomato fluorescence using whole-animal imaging with an IVIS system (Perkin Elmer). .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Protein Concentration:

Article Title: Expression of Human Apolipoprotein E3 or E4 in the Brains ofApoe−/− Mice: Isoform-Specific Effects on Neurodegeneration
Article Snippet: After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions. .. After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions.

Injection:

Article Title: Analysis of cilia dysfunction phenotypes in zebrafish embryos depleted of Origin recognition complex factors
Article Snippet: Injected zebrafish embryos were raised until 8 somite stage (ss) and fixed overnight at 4 ºC in 4 % PFA. .. Fixed embryos were dechorionated, washed with PBS containing 0.1 % Tween-20 and processed for cilia staining as described using an anti-acetylated tubulin antibody (Sigma, Munich, Germany, clone 6-11B-1, 1:1000).

Binding Assay:

Article Title: Expression of Human Apolipoprotein E3 or E4 in the Brains ofApoe−/− Mice: Isoform-Specific Effects on Neurodegeneration
Article Snippet: After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions. .. After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions.

Nucleic Acid Electrophoresis:

Article Title: mRNAs biotinylated within the 5′ cap and protected against decapping: new tools to capture RNA–protein complexes
Article Snippet: The supernatants were collected, mixed with 6X protein loading buffer and proteins were separated by SDS-polyacrylamide gel electrophoresis and electrotransferred onto nitrocellulose membranes (GE Healthcare). .. Membranes were washed three times with PBS supplemented with 0.2% Tween 20 and incubated for 1 h with horseradish-peroxidase-conjugated rat secondary antibodies (Sigma-Aldrich, St Louis, MO) diluted 1 : 10 000.

In Vivo:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: Paragraph title: 2.12. Wnt signaling inhibition - in vivo luminescence/fluorescence ... Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Fluorescence:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: One week following transplantation, mice were treated with 100 mg/kg D-Luciferin via I.P. administration (Perkin Elmer), and tumor burden was quantified by detecting tdTomato fluorescence using whole-animal imaging with an IVIS system (Perkin Elmer). .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Transfection:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: A KPT cell line was transfected with 7TCF:Luciferase-PGK:Puro lentiviruses Addgene # 24308, which becomes positive for firefly luciferase upon activation of the Wnt pathway [ ]. .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Mouse Assay:

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes. .. Intestinal tissue was harvested from mice and fixed in 10% formalin for 24 h and was then processed for paraffin inclusion.

Homogenization:

Article Title: Expression of Human Apolipoprotein E3 or E4 in the Brains ofApoe−/− Mice: Isoform-Specific Effects on Neurodegeneration
Article Snippet: After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions. .. After another three 10 min washes with PBS containing 0.05% Tween 20, the reaction was developed with o -phenylenediamine dihydrochloride peroxidase substrate tablets (Sigma) per manufacturer’s instructions.

Activation Assay:

Article Title: The T cell antigen receptor α transmembrane domain coordinates triggering through regulation of bilayer immersion and CD3 subunit associations
Article Snippet: Paragraph title: SMSC analysis, force-bond lifetime measurements, and induced T cell activation by optical traps ... After washing with PBST buffer (1X PBS + 0.02% (v/v) Tween-20) two times, the bead slurry was diluted 200-fold with 5 mg/mL Bovine serum albumin (BSA) (Sigma-Aldrich) in colorless DMEM medium (Sigma-Aldrich) for bond lifetime measurements.

Article Title: Potent in vivo lung cancer Wnt signaling inhibition via cyclodextrin-LGK974 inclusion complexes
Article Snippet: Wnt signaling activity was quantified by normalizing the luciferase signal generated by Wnt activation to the tdTomato signal generated by the tumor burden. .. Mice were treated with 5 mg/kg/d of LGK974 resuspended in 0.5% carboxymethylcellulose (Sigma) and 0.5% Tween-80 (Sigma) or CD:LGK974 complexes.

Staining:

Article Title: Analysis of cilia dysfunction phenotypes in zebrafish embryos depleted of Origin recognition complex factors
Article Snippet: .. Fixed embryos were dechorionated, washed with PBS containing 0.1 % Tween-20 and processed for cilia staining as described using an anti-acetylated tubulin antibody (Sigma, Munich, Germany, clone 6-11B-1, 1:1000). .. For KV detection an antibody against PKCζ (Santa Cruz Biotechnology, Heidelberg, Germany, catalog no. sc-216, 1:500) was used, which labels apical cell borders and allows for detection of the KV outline.

other:

Article Title: Characterization of Aloe vera-chitosan composite films and their use for reducing the disease caused by fungi in papaya Maradol
Article Snippet: Chitosan (85% deacetylated, MW = 340.33), glacial acetic acid, Tween 20 (Sigma-Aldrich® ) and glycerol (Meyer® ) were used.

Article Title: High-Throughput Production of Micrometer Sized Double Emulsions and Microgel Capsules in Parallelized 3D Printed Microfluidic Devices
Article Snippet: Materials Paraffin oil, Hexane, Isopropanol, TWEEN 80, SPAN 80, Irgacure 2959 and FITC-Dextran (10 kDa) were purchased from Sigma-Aldrich (Munich, Germany).

Article Title: Decontamination Effect of the Spindle and 222-Nanometer Krypton-Chlorine Excimer Lamp Combination against Pathogens on Apples (Malus domestica Borkh.) and Bell Peppers (Capsicum annuum L.)
Article Snippet: Moreover, the single Spindle or Sp-Ex treatment with surfactant was performed based on the procedure described above, either with or without operation of the 222-nm KrCl excilamp or use of Tween 20 (Sigma, St. Louis, MO, USA) diluted to 0.1% in sterile tap water.

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  • 88
    Millipore goat anti sod1
    Immunohistochemistry for superoxide dismutase <t>(SOD1)</t> (a-c), manganese superoxide dismutase (SOD2) (d-f), catalase (CAT) (g-i) and glutathione peroxidase (GPx) (j-l) in the rat liver of the normal-(left column), ascorbic acid - (middle column) and Oenanthe javanica extract (OJE) - (right column) groups. Strong SOD1, SOD2, CAT, and GPx immunoreactive cells (arrows) are markedly increased in the OJE-group. Scale bar = 120 μ m.
    Goat Anti Sod1, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti sod1/product/Millipore
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    goat anti sod1 - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    96
    Millipore glut1
    Myocardial glucose and lipid metabolism in MHC-D2 mice. Basal and insulin-stimulated glucose uptake in heart was measured following iv injection of 2-[ 14 C]DG in MHC-D2 mice and WT littermates. (A) Insulin-stimulated glucose uptake in heart (n = 4 for each group). (B) Basal glucose uptake in heart (n = 7 for each group). (C) Myocardial triglyceride levels (n = 4–6). (D) <t>GLUT1</t> expression in heart samples was measured using Western blot. (E) Systemic clearance of [ 3 H]-palmitate following a bolus injection (n = 4 for each group). (F) 3 H-glucose conversion into myocardial triglyceride (n = 4–6). (G) Basal glucose uptake in white adipose tissue (epididymal) (n = 3 for each group). (H) Intrahepatic triglyceride levels (n = 4–6). Data are presented as means ± SE. *, P
    Glut1, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glut1/product/Millipore
    Average 96 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    glut1 - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    86
    Millipore anti ngfr p75 polyclonal antibody
    <t>NGFR-p75,</t> TrkA and NGF protein levels in hippocampus (HPP). Mice were treated as in Fig. 4 . NGFR-p75 (** p
    Anti Ngfr P75 Polyclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ngfr p75 polyclonal antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ngfr p75 polyclonal antibody - by Bioz Stars, 2020-04
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      Buy from Supplier

    Image Search Results


    Immunohistochemistry for superoxide dismutase (SOD1) (a-c), manganese superoxide dismutase (SOD2) (d-f), catalase (CAT) (g-i) and glutathione peroxidase (GPx) (j-l) in the rat liver of the normal-(left column), ascorbic acid - (middle column) and Oenanthe javanica extract (OJE) - (right column) groups. Strong SOD1, SOD2, CAT, and GPx immunoreactive cells (arrows) are markedly increased in the OJE-group. Scale bar = 120 μ m.

    Journal: Chinese Medical Journal

    Article Title: Effect of Oenanthe Javanica Extract on Antioxidant Enzyme in the Rat Liver

    doi: 10.4103/0366-6999.158363

    Figure Lengend Snippet: Immunohistochemistry for superoxide dismutase (SOD1) (a-c), manganese superoxide dismutase (SOD2) (d-f), catalase (CAT) (g-i) and glutathione peroxidase (GPx) (j-l) in the rat liver of the normal-(left column), ascorbic acid - (middle column) and Oenanthe javanica extract (OJE) - (right column) groups. Strong SOD1, SOD2, CAT, and GPx immunoreactive cells (arrows) are markedly increased in the OJE-group. Scale bar = 120 μ m.

    Article Snippet: To reduce background staining, the membranes were incubated with 5% nonfat dry milk in PBS containing 0.1% Tween 20 for 45 min, followed by incubation with goat anti-SOD1 (diluted 1:1000, Calbiochem, Darmstadt, Germany), goat anti-SOD2 (diluted 1:1000, Calbiochem), rabbit anti-CAT (diluted 1:1000, LabFrontier, Seoul, Korea) and sheep anti-GPx (diluted 1:1000, Chemicon International, Billerica, MA), peroxidase-conjugated donkey anti-goat IgG, goat anti-rabbit IgG or goat anti-sheep IgG (Santa Cruz, USA), and an ECL kit (Pierce Chemical, Rockford, USA).

    Techniques: Immunohistochemistry

    Myocardial glucose and lipid metabolism in MHC-D2 mice. Basal and insulin-stimulated glucose uptake in heart was measured following iv injection of 2-[ 14 C]DG in MHC-D2 mice and WT littermates. (A) Insulin-stimulated glucose uptake in heart (n = 4 for each group). (B) Basal glucose uptake in heart (n = 7 for each group). (C) Myocardial triglyceride levels (n = 4–6). (D) GLUT1 expression in heart samples was measured using Western blot. (E) Systemic clearance of [ 3 H]-palmitate following a bolus injection (n = 4 for each group). (F) 3 H-glucose conversion into myocardial triglyceride (n = 4–6). (G) Basal glucose uptake in white adipose tissue (epididymal) (n = 3 for each group). (H) Intrahepatic triglyceride levels (n = 4–6). Data are presented as means ± SE. *, P

    Journal: Endocrinology

    Article Title: Cardiac Expression of Human Type 2 Iodothyronine Deiodinase Increases Glucose Metabolism and Protects Against Doxorubicin-induced Cardiac Dysfunction in Male Mice

    doi: 10.1210/en.2012-2261

    Figure Lengend Snippet: Myocardial glucose and lipid metabolism in MHC-D2 mice. Basal and insulin-stimulated glucose uptake in heart was measured following iv injection of 2-[ 14 C]DG in MHC-D2 mice and WT littermates. (A) Insulin-stimulated glucose uptake in heart (n = 4 for each group). (B) Basal glucose uptake in heart (n = 7 for each group). (C) Myocardial triglyceride levels (n = 4–6). (D) GLUT1 expression in heart samples was measured using Western blot. (E) Systemic clearance of [ 3 H]-palmitate following a bolus injection (n = 4 for each group). (F) 3 H-glucose conversion into myocardial triglyceride (n = 4–6). (G) Basal glucose uptake in white adipose tissue (epididymal) (n = 3 for each group). (H) Intrahepatic triglyceride levels (n = 4–6). Data are presented as means ± SE. *, P

    Article Snippet: Membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 for 1 hour at room temperature and incubated with GLUT1 (Millipore, Billerica, Massachusetts), GLUT4 (Cell Signaling, Danvers, Massachusetts), Thr172 -phospho-AMPK (Cell Signaling), AMPK (Cell Signaling), Ser473 -phospho-Akt (Cell Signaling), or Akt (Cell Signaling) antibodies in Tris-buffered saline with Tween 20 containing 5% skim milk or BSA for overnight at 4°C.

    Techniques: Mouse Assay, IV Injection, Expressing, Western Blot, Injection

    Metabolic signaling pathways following DOX treatment in MHC-D2 and WT mice. Metabolic signaling pathways were examined using Western blot analysis in heart samples obtained at baseline and following DOX treatment in MHC-D2 and WT mice (n = 3–4 for each group). (A) Thr 172 -phosphorylation of AMPK and GLUT4 protein levels in heart of WT and MHC-D2 mice at baseline and after DOX treatment. (B) GLUT1 protein levels in heart of MHC-D2 mice at baseline and after DOX treatment. (C) Total Akt protein levels in heart of WT mice at baseline and after DOX treatment. (D) Ser 473 -phosphorylation of AKT in heart of WT mice. (E) Ratio of Ser 473 -phosphorylation of AKT and total Akt protein levels in WT mice. Data are presented as means ± SE. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading controls for all data. *, P

    Journal: Endocrinology

    Article Title: Cardiac Expression of Human Type 2 Iodothyronine Deiodinase Increases Glucose Metabolism and Protects Against Doxorubicin-induced Cardiac Dysfunction in Male Mice

    doi: 10.1210/en.2012-2261

    Figure Lengend Snippet: Metabolic signaling pathways following DOX treatment in MHC-D2 and WT mice. Metabolic signaling pathways were examined using Western blot analysis in heart samples obtained at baseline and following DOX treatment in MHC-D2 and WT mice (n = 3–4 for each group). (A) Thr 172 -phosphorylation of AMPK and GLUT4 protein levels in heart of WT and MHC-D2 mice at baseline and after DOX treatment. (B) GLUT1 protein levels in heart of MHC-D2 mice at baseline and after DOX treatment. (C) Total Akt protein levels in heart of WT mice at baseline and after DOX treatment. (D) Ser 473 -phosphorylation of AKT in heart of WT mice. (E) Ratio of Ser 473 -phosphorylation of AKT and total Akt protein levels in WT mice. Data are presented as means ± SE. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading controls for all data. *, P

    Article Snippet: Membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 for 1 hour at room temperature and incubated with GLUT1 (Millipore, Billerica, Massachusetts), GLUT4 (Cell Signaling, Danvers, Massachusetts), Thr172 -phospho-AMPK (Cell Signaling), AMPK (Cell Signaling), Ser473 -phospho-Akt (Cell Signaling), or Akt (Cell Signaling) antibodies in Tris-buffered saline with Tween 20 containing 5% skim milk or BSA for overnight at 4°C.

    Techniques: Mouse Assay, Western Blot

    NGFR-p75, TrkA and NGF protein levels in hippocampus (HPP). Mice were treated as in Fig. 4 . NGFR-p75 (** p

    Journal: PLoS ONE

    Article Title: BMP9 Protects Septal Neurons from Axotomy-Evoked Loss of Cholinergic Phenotype

    doi: 10.1371/journal.pone.0021166

    Figure Lengend Snippet: NGFR-p75, TrkA and NGF protein levels in hippocampus (HPP). Mice were treated as in Fig. 4 . NGFR-p75 (** p

    Article Snippet: After transfer of protein to an Immoblon P membrane (Millipore), the membrane was blocked with 5% nonfat dry milk in 1× Tris-buffered saline (TBS) containing 0.1% Tween 20 for 2 h and then probed overnight with either anti-NGFR-p75 polyclonal antibody (1∶1000) (Adavanced Targeting Systems); anti-CHAT polyclonal antibody (1∶1000) (Millipore); anti-CHT polyclonal antibody (1∶1000) (Millipore); anti-TrkA polyclonal antibody (1∶1000) (Millipore) or anti-β-actin monoclonal A5441 antibody (1∶5000) (Sigma).

    Techniques: Mouse Assay