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Thermo Fisher ttbs
Ttbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ttbs/product/Thermo Fisher
Average 94 stars, based on 163 article reviews
Price from $9.99 to $1999.99
ttbs - by Bioz Stars, 2020-07
94/100 stars

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Incubation:

Article Title: Increased oxidative stress and apoptosis in the hypothalamus of diabetic male mice in the insulin receptor substrate-2 knockout model
Article Snippet: .. The membranes were washed three times with TTBS and incubated with the corresponding secondary antibody conjugated with peroxidase (Thermo Fisher Scientific Inc., Waltham, MA) at a dilution of 1:2000 in non-fat milk during 90 min at 25°C. .. The proteins were detected by chemiluminescence using an immune-star western chemiluminiscent kit (Bio-Rad) and quantified by densitometry using a Kodak Gel Logic 1500 Image Analysis system and Molecular Imaging Software version 4.0 (Rochester, NY).

Article Title: An Immunological Analysis of Dystroglycan Subunits: Lessons Learned from a Small Cohort of Non-Congenital Dystrophic Patients
Article Snippet: .. After washing in TTBS and in TTBS containing 3% milk powder for 30 min, the blots were incubated for 1 h with anti-mouse or anti-rabbit antibodies horseradish peroxidase-conjugated (Pierce). .. After washing in 3% milk powder TTBS and several washes in TTBS, blots were developed using enhanced chemiluminescence (ECL) (Pierce).

Article Title: Distinct Dasatinib-Induced Mechanisms of Apoptotic Response and Exosome Release in Imatinib-Resistant Human Chronic Myeloid Leukemia Cells
Article Snippet: .. After washing three times with TTBS, membranes were incubated with HRP-conjugated goat anti-rabbit or mouse antibody (1:10,000; Thermo Scientific) for 1 h, and then developed with the enhanced chemiluminescence reagent (Thermo Scientific). ..

Article Title: Intracellular Ca2+ release through ryanodine receptors contributes to AMPA receptor-mediated mitochondrial dysfunction and ER stress in oligodendrocytes
Article Snippet: .. After washing, membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1 : 2000, Sigma) in 5% skimmed milk, 1% normal serum in TTBS for 2-h RT and developed using enhanced chemiluminiscence according to the manufacturer's instructions (SuperSignal West Dura, Pierce, Rockford, IL, USA). .. Signals were quantified using Image-J software (NIH, Bethesda, MA, USA) and values were normalized to β -actin signal and provided as the mean±S.E.M. of at least three independent experiments.

Article Title: Salmonella-Induced Caspase-2 Activation in Macrophages
Article Snippet: .. After 1 h blocking in TTBS (10 mM Tris-HCl, pH 8.0; 150 mM sodium chloride; 0.1% Tween 20) supplemented with 5% milk powder, the membranes were probed with the appropriate primary antibodies (actin, caspase-1, caspase-2, and caspase-3 from Santa Cruz Biotechnology, Inc.; caspase-6 and caspase-8 from Chemicon; cytochrome c from BD PharMingen; and cytochrome c oxidase subunit IV [cox-IV] from Molecular Probes) diluted in 1% BSA (fraction V; Sigma-Aldrich) in TTBS before incubation with peroxidase-conjugated secondary antibodies and detection by an enhanced chemiluminescence system (Pierce Chemical Co.). .. Subcellular Fractionation.

Expressing:

Article Title: Mangiferin Prevents Guinea Pig Tracheal Contraction via Activation of the Nitric Oxide-Cyclic GMP Pathway
Article Snippet: .. The membranes were washed in TTBS and protein expression was detected using enhanced chemiluminescence (SuperSignal West Dura, Thermo Fisher Scientific Inc., Rockford, USA). .. Bands intensity was quantified by densitometry (Image-Pro® Plus Media Cybernetics, Bethesda, MD).

Blocking Assay:

Article Title: Salmonella-Induced Caspase-2 Activation in Macrophages
Article Snippet: .. After 1 h blocking in TTBS (10 mM Tris-HCl, pH 8.0; 150 mM sodium chloride; 0.1% Tween 20) supplemented with 5% milk powder, the membranes were probed with the appropriate primary antibodies (actin, caspase-1, caspase-2, and caspase-3 from Santa Cruz Biotechnology, Inc.; caspase-6 and caspase-8 from Chemicon; cytochrome c from BD PharMingen; and cytochrome c oxidase subunit IV [cox-IV] from Molecular Probes) diluted in 1% BSA (fraction V; Sigma-Aldrich) in TTBS before incubation with peroxidase-conjugated secondary antibodies and detection by an enhanced chemiluminescence system (Pierce Chemical Co.). .. Subcellular Fractionation.

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  • 93
    Thermo Fisher v1v2 zm109 ttb
    Immunogenicity of Multivalent <t>V1V2-Scaffold</t> Proteins in Macaques (A) Immunization protocols and NHP groups with timelines and regimens. (B) Individual group strategies for immunogenicity studies using DNA and protein co-immunizations. (C) Animal IDs and structural models of V1V2- and V3-scaffold immunogens, with icons representing protein immunogens. Scaffold structural models are shown in gray, and engrafted V1V2 or V3 regions are colored. <t>TTB,</t> typhoid toxin B subunit; monFc, monkey IgG Fc.
    V1v2 Zm109 Ttb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v1v2 zm109 ttb/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    v1v2 zm109 ttb - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    94
    Thermo Fisher ttbs
    Immunogenicity of Multivalent <t>V1V2-Scaffold</t> Proteins in Macaques (A) Immunization protocols and NHP groups with timelines and regimens. (B) Individual group strategies for immunogenicity studies using DNA and protein co-immunizations. (C) Animal IDs and structural models of V1V2- and V3-scaffold immunogens, with icons representing protein immunogens. Scaffold structural models are shown in gray, and engrafted V1V2 or V3 regions are colored. <t>TTB,</t> typhoid toxin B subunit; monFc, monkey IgG Fc.
    Ttbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ttbs/product/Thermo Fisher
    Average 94 stars, based on 163 article reviews
    Price from $9.99 to $1999.99
    ttbs - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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    Immunogenicity of Multivalent V1V2-Scaffold Proteins in Macaques (A) Immunization protocols and NHP groups with timelines and regimens. (B) Individual group strategies for immunogenicity studies using DNA and protein co-immunizations. (C) Animal IDs and structural models of V1V2- and V3-scaffold immunogens, with icons representing protein immunogens. Scaffold structural models are shown in gray, and engrafted V1V2 or V3 regions are colored. TTB, typhoid toxin B subunit; monFc, monkey IgG Fc.

    Journal: Cell reports

    Article Title: Multimeric Epitope-Scaffold HIV Vaccines Target V1V2 and Differentially Tune Polyfunctional Antibody Responses

    doi: 10.1016/j.celrep.2019.06.074

    Figure Lengend Snippet: Immunogenicity of Multivalent V1V2-Scaffold Proteins in Macaques (A) Immunization protocols and NHP groups with timelines and regimens. (B) Individual group strategies for immunogenicity studies using DNA and protein co-immunizations. (C) Animal IDs and structural models of V1V2- and V3-scaffold immunogens, with icons representing protein immunogens. Scaffold structural models are shown in gray, and engrafted V1V2 or V3 regions are colored. TTB, typhoid toxin B subunit; monFc, monkey IgG Fc.

    Article Snippet: Using the assay developed by (109), 5 μg of V1V2(ZM53)-2F5K, V1V2(ZM109)-1FD6, or V1V2(ZM109)-TTB were biotinylated using EZ-Link Sulfo-NHS-LC-LC-Biotin (Thermo Scientific) and then conjugated to fluorescent neutravidin beads (Thermo) according to manufacturer’s instructions.

    Techniques:

    Epitope-Specific mAbs Define the Antigenicity of V1V2 and V3 Scaffolds V1V2-specific mAbs were serially diluted and assayed by ELISA in duplicate at a starting concentration of 5 μg/mL to evaluate the antigenicity of each immunogen. Curves are mean ± SEM. (A-F). Each mAb and specificity is shown: PG9 V2q (A), 830A V2i (B), 2158 V2i (C), 697D V2i (D), CH58 V2p (E), CH59 V2p (F). Unique symbols identify each V1V2-scaffold protein. (G) Binding to the V3(ZM109)-TTB scaffold with V3-specific mAbs 447–52D and 2219 and control V2 and CD4bs mAbs. (H) EC 50 values derived from ELISA curves in (A)–(G). Data are representative of assays repeated at least twice with comparable results.

    Journal: Cell reports

    Article Title: Multimeric Epitope-Scaffold HIV Vaccines Target V1V2 and Differentially Tune Polyfunctional Antibody Responses

    doi: 10.1016/j.celrep.2019.06.074

    Figure Lengend Snippet: Epitope-Specific mAbs Define the Antigenicity of V1V2 and V3 Scaffolds V1V2-specific mAbs were serially diluted and assayed by ELISA in duplicate at a starting concentration of 5 μg/mL to evaluate the antigenicity of each immunogen. Curves are mean ± SEM. (A-F). Each mAb and specificity is shown: PG9 V2q (A), 830A V2i (B), 2158 V2i (C), 697D V2i (D), CH58 V2p (E), CH59 V2p (F). Unique symbols identify each V1V2-scaffold protein. (G) Binding to the V3(ZM109)-TTB scaffold with V3-specific mAbs 447–52D and 2219 and control V2 and CD4bs mAbs. (H) EC 50 values derived from ELISA curves in (A)–(G). Data are representative of assays repeated at least twice with comparable results.

    Article Snippet: Using the assay developed by (109), 5 μg of V1V2(ZM53)-2F5K, V1V2(ZM109)-1FD6, or V1V2(ZM109)-TTB were biotinylated using EZ-Link Sulfo-NHS-LC-LC-Biotin (Thermo Scientific) and then conjugated to fluorescent neutravidin beads (Thermo) according to manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Binding Assay, Derivative Assay

    V1V2-Scaffold Immunogens Induce Neutralizing Antibody Titers that Correlate with Increasing Antibody Affinity Neutralization data are expressed as the ID 50 ; i.e., the plasma dilution that neutralized 50% of the infecting virus in the TZM-bl assay. (A) Individual ID 50 values from plasma samples collected 2 weeks after each immunization, tested in duplicate for neutralization of tier 1B ZM109 and tier 1A MW965 and SF162 pseudoviruses. (B) ID 50 values for each animal in each group and the mean ID 50 +/− SEM for each group (n = 6) using week 22 plasma against tier 1 clade B SHIV BaL and tier 1 clade C SHIV 1157-ipEL . (C) Group (n = 6) mean (± SEM) SHIV ID 50 neutralizing titers in plasma. Data are representative of assays that were repeated at least twice with comparable results. (D–F) Correlations between HIV(ZM109) neutralization (ID 50 ) and Luminex binding (MFI) for V1V2(YU2)-1FD6 (D), V1V2(ZM109)-1FD6 (E), and V1V2(1086) (F) tags. (G) Correlation between SHIV 1157.ipEL neutralization (ID 50 ) and BLI off-rates (s −1 ) for V1V2(1086) tags. Spearman correlation tests were performed in GraphPad Prism 7.0 for Mac.

    Journal: Cell reports

    Article Title: Multimeric Epitope-Scaffold HIV Vaccines Target V1V2 and Differentially Tune Polyfunctional Antibody Responses

    doi: 10.1016/j.celrep.2019.06.074

    Figure Lengend Snippet: V1V2-Scaffold Immunogens Induce Neutralizing Antibody Titers that Correlate with Increasing Antibody Affinity Neutralization data are expressed as the ID 50 ; i.e., the plasma dilution that neutralized 50% of the infecting virus in the TZM-bl assay. (A) Individual ID 50 values from plasma samples collected 2 weeks after each immunization, tested in duplicate for neutralization of tier 1B ZM109 and tier 1A MW965 and SF162 pseudoviruses. (B) ID 50 values for each animal in each group and the mean ID 50 +/− SEM for each group (n = 6) using week 22 plasma against tier 1 clade B SHIV BaL and tier 1 clade C SHIV 1157-ipEL . (C) Group (n = 6) mean (± SEM) SHIV ID 50 neutralizing titers in plasma. Data are representative of assays that were repeated at least twice with comparable results. (D–F) Correlations between HIV(ZM109) neutralization (ID 50 ) and Luminex binding (MFI) for V1V2(YU2)-1FD6 (D), V1V2(ZM109)-1FD6 (E), and V1V2(1086) (F) tags. (G) Correlation between SHIV 1157.ipEL neutralization (ID 50 ) and BLI off-rates (s −1 ) for V1V2(1086) tags. Spearman correlation tests were performed in GraphPad Prism 7.0 for Mac.

    Article Snippet: Using the assay developed by (109), 5 μg of V1V2(ZM53)-2F5K, V1V2(ZM109)-1FD6, or V1V2(ZM109)-TTB were biotinylated using EZ-Link Sulfo-NHS-LC-LC-Biotin (Thermo Scientific) and then conjugated to fluorescent neutravidin beads (Thermo) according to manufacturer’s instructions.

    Techniques: Neutralization, Luminex, Binding Assay