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Promega ttbs
Ttbs, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ttbs/product/Promega
Average 92 stars, based on 9 article reviews
Price from $9.99 to $1999.99
ttbs - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Western Blot:

Article Title: Glutathione Levels and Susceptibility to Chemically Induced Injury in Two Human Prostate Cancer Cell Lines
Article Snippet: .. The blots were washed three times with Tris-buffered saline containing Tween 20 (TTBS) and incubated with the appropriate secondary antibody conjugated to alkaline phosphatase (Jackson ImmunoResearch; West Grove, PA, USA) or horseradish peroxidase for 1 h. Blots were washed 3–6 times in TTBS and then assayed for color development using 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (BCIP/NBT) as substrates (Promega) or exposed for visualization on autoradiography film (Denville Scientific; Metuchen, PA, USA) using the Pierce enhanced chemiluminescence (ECL) Western Blotting Substrate Kit (Pierce; Rockford, IL, USA). .. For all blots, the order of loading was: Lane 1 = PC-3 cells; Lane 2 = LNCaP cells; Lane 3 = molecular weight markers.

Incubation:

Article Title: Genome editing in mammals using CRISPR type I-D nuclease
Article Snippet: .. The primary antibody reactions were performed at room temperature for 60 min. After the membranes were washed by TTBS for 5 min 3 times, the secondary antibody, Anti-Mouse IgG (H+L), HRP Conjugate (Promega, 1:10000 in Blocking one), was added to the membrane, After incubation for 60 min at room temperature, the membrane was washed with TTBS 5 min 3 times. .. Signal was detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) according to the protocol provided by the manufacturer.

Article Title: Antiallodynic Effects of Cannabinoid Receptor 2 (CB2R) Agonists on Retrovirus Infection-Induced Neuropathic Pain
Article Snippet: .. After washing 3x with TTBS, alkaline phosphatase (AP) conjugated secondary antibody (1 : 5,000 in 1% Blotto, Promega, Madison, WI) was added to the membranes at RT for 1 h. Following 3x washing with TTBS, membranes were rinsed in the assay buffer (1x) twice (2 min each) and then incubated in the substrate solution (CDP-Star, Applied Biosystems, Foster City, CA) for 10 min followed by imaging using an Odyssey Imaging System (LI-COR Biosciences, Lincoln, NE). ..

Article Title: Glutathione Levels and Susceptibility to Chemically Induced Injury in Two Human Prostate Cancer Cell Lines
Article Snippet: .. The blots were washed three times with Tris-buffered saline containing Tween 20 (TTBS) and incubated with the appropriate secondary antibody conjugated to alkaline phosphatase (Jackson ImmunoResearch; West Grove, PA, USA) or horseradish peroxidase for 1 h. Blots were washed 3–6 times in TTBS and then assayed for color development using 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (BCIP/NBT) as substrates (Promega) or exposed for visualization on autoradiography film (Denville Scientific; Metuchen, PA, USA) using the Pierce enhanced chemiluminescence (ECL) Western Blotting Substrate Kit (Pierce; Rockford, IL, USA). .. For all blots, the order of loading was: Lane 1 = PC-3 cells; Lane 2 = LNCaP cells; Lane 3 = molecular weight markers.

Article Title: Nitrosative damage during retrovirus infection-induced neuropathic pain
Article Snippet: .. After washing 3× with TTBS, membranes were incubated in alkaline phosphatase (AP) conjugated-secondary antibody (1:5000 in 1% blotto, Promega) at room temperature for 1 h. Membrane blots were washed 3× with TTBS followed by 2× assay buffer (1×) and then were incubated in substrate solution (CDP-Star, Applied Biosystems, now Thermal Fisher) for 10 min. ..

Article Title: The immunomodulating V and W proteins of Nipah virus determine disease course
Article Snippet: .. The membranes were then incubated with anti-rabbit IgG conjugated to horseradish peroxidase (Sigma-Aldrich; 1:20,000 dilution) for 1 h at room temperature, washed three times in TTBS, incubated with ECL reagent (Promega) for 5 min and imaged with a VersaDoc (Bio-Rad). .. Virus growth kinetics Overall, 1.2 × 106 cells per well of 293T or Vero cells were seeded in six-well plates and incubated at 37 °C for 12 h with either regular medium, or medium containing 1,000 U ml−1 of Universal IFN-α (PBL Assay Science, Piscataway, NJ).

Article Title: Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response
Article Snippet: .. After washing coated plates 3 times with Tris-Tween buffered saline (TTBS), serum samples (100 μ L, 1 : 50 dilution in TTBS plus 1 mg/mL bovine haemoglobin) were added to each well, and after 1-hour incubation at 37°C and three washes with TTBS, the wells were incubated with alkaline phosphatase-conjugated anti-mouse antibody (1 : 1000 dilution in TTBS, Lot number 0000103573, Promega, Madison, WI USA). .. Alkaline phosphatase substrate (1 mg NPP/mL, Lot number 1001595631, Sigma, Spruce St., USA) was then added, and absorbance was measured at 405 nm using a Tecan Sunrise® plate reader (Tecan, Grödig, Austria).

Article Title: Modulation of dendritic spines and synaptic function by Rac1: A possible link to Fragile X syndrome pathology
Article Snippet: .. Blots were washed three times for 15 min in TTBS, followed by incubation in the corresponding secondary antibody (horseradish peroxidase-conjugated goat anti-mouse IgG [1:5,000] from Promega, Madison, WI) for Rac1 and Cdc42; anti-rabbit for RhoA, RhoB, total PAK1 and phosphorylated PAK1, total and phosphorylated ERK. .. This was followed by washing three times in 1X TTBS and visualized using enhanced chemiluminescence (ECL, Amersham Biosciences, Piscataway, NJ) and by exposing the blots to X-Omat Blue film (Kodak, Rochester, NY) for ~ 15 s to 1 min. Densitometric analysis of all samples were performed using NIH Image J software.

Autoradiography:

Article Title: Glutathione Levels and Susceptibility to Chemically Induced Injury in Two Human Prostate Cancer Cell Lines
Article Snippet: .. The blots were washed three times with Tris-buffered saline containing Tween 20 (TTBS) and incubated with the appropriate secondary antibody conjugated to alkaline phosphatase (Jackson ImmunoResearch; West Grove, PA, USA) or horseradish peroxidase for 1 h. Blots were washed 3–6 times in TTBS and then assayed for color development using 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (BCIP/NBT) as substrates (Promega) or exposed for visualization on autoradiography film (Denville Scientific; Metuchen, PA, USA) using the Pierce enhanced chemiluminescence (ECL) Western Blotting Substrate Kit (Pierce; Rockford, IL, USA). .. For all blots, the order of loading was: Lane 1 = PC-3 cells; Lane 2 = LNCaP cells; Lane 3 = molecular weight markers.

Imaging:

Article Title: Antiallodynic Effects of Cannabinoid Receptor 2 (CB2R) Agonists on Retrovirus Infection-Induced Neuropathic Pain
Article Snippet: .. After washing 3x with TTBS, alkaline phosphatase (AP) conjugated secondary antibody (1 : 5,000 in 1% Blotto, Promega, Madison, WI) was added to the membranes at RT for 1 h. Following 3x washing with TTBS, membranes were rinsed in the assay buffer (1x) twice (2 min each) and then incubated in the substrate solution (CDP-Star, Applied Biosystems, Foster City, CA) for 10 min followed by imaging using an Odyssey Imaging System (LI-COR Biosciences, Lincoln, NE). ..

Blocking Assay:

Article Title: Genome editing in mammals using CRISPR type I-D nuclease
Article Snippet: .. The primary antibody reactions were performed at room temperature for 60 min. After the membranes were washed by TTBS for 5 min 3 times, the secondary antibody, Anti-Mouse IgG (H+L), HRP Conjugate (Promega, 1:10000 in Blocking one), was added to the membrane, After incubation for 60 min at room temperature, the membrane was washed with TTBS 5 min 3 times. .. Signal was detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) according to the protocol provided by the manufacturer.

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  • 85
    Promega b ttbs
    B Ttbs, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b ttbs/product/Promega
    Average 85 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    b ttbs - by Bioz Stars, 2020-07
    85/100 stars
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