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Bio-Rad ttbs
Ttbs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ttbs/product/Bio-Rad
Average 99 stars, based on 259 article reviews
Price from $9.99 to $1999.99
ttbs - by Bioz Stars, 2020-07
99/100 stars

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Electrophoresis:

Article Title: A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts
Article Snippet: .. Two-dimensional electrophoresis Samples containing 75–200 μg of fractionated cytoplasmic proteins were diluted in 2D electrophoresis buffer (2 M thiourea, 5 M urea, 0.25% CHAPS, 0.25% Tween-20, 0.25% SB-3, 10% isopropanol, and 12.5% water-saturated butanol) containing 15 mg DTT and 0.5% ampholytes (pI ranges 3–10 or 5–8; Bio-Rad; Hercules, CA) to a final volume of 0.2 ml, and passively absorbed onto immobilized pH gradient strips (pI ranges 5–8 or 3–10 NL (non-linear); Bio-Rad) for 4 hours at 20°C followed by active rehydration (50 V at 20°C) for 8 hours. .. Isoelectric focusing was performed for 35,000 V-h on a Protean IEF System (Bio-Rad), electrophoresis with the Criterion gel system (Bio-Rad).

Incubation:

Article Title: In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the Type II secretion system of Vibrio cholerae
Article Snippet: .. Membranes were incubated with polyclonal antisera against EpsG (1:150,000 in Tris-buffered saline with 0.1% Tween-20) or EpsL (1:40,000 in Tris buffered saline with 0.1% Tween-20), followed by horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (BioRad) diluted 1:15,000 in Tris-buffered saline containing 0.1% Tween-20. .. Blots were developed with ECL Plus Western blotting detection reagent (GE Healthcare) and protein was visualized using a Typhoon Trio variable mode imager system and Image Quant software.

Article Title: Regulatory landscape of AGE-RAGE-oxidative stress axis and its modulation by PPARγ activation in high fructose diet-induced metabolic syndrome
Article Snippet: .. The blots were incubated in immunoblotting buffer (TBST: 20 mM Tris-HCl, pH 7.4, 0.15 M NaCl, 0.05% Tween-20) (Bio-Rad) containing 5% (w/v) Skim Milk Powder at room temperature, followed by washing with TBST. .. Blots were then incubated overnight at 4 °C with either anti-CML antibodies (1:800) in TBST containing 5% milk, or Anti-HNE polyclonal antibody (1:800) or anti-RAGE antibodies (1:400) both in TBST containing 3% milk.

other:

Article Title: Crystal Structures of Major Envelope Proteins VP26 and VP28 from White Spot Syndrome Virus Shed Light on Their Evolutionary Relationship ▿
Article Snippet: Nevertheless, when treated with Tween 20, VP26 may become accessible.

Article Title: Crystal Structures of Major Envelope Proteins VP26 and VP28 from White Spot Syndrome Virus Shed Light on Their Evolutionary Relationship ▿
Article Snippet: To clarify the location of VP26 in the virion, virus treatment with Tween 20 gave a mixture of partial and complete separation of the viral envelope from the nucleocapsid to reveal the location of the bound gold particles.

Labeling:

Article Title: Crystal Structures of Major Envelope Proteins VP26 and VP28 from White Spot Syndrome Virus Shed Light on Their Evolutionary Relationship ▿
Article Snippet: .. Our result showed that 0.1% Tween 20 could gently separate the envelope from the nucleocapsid, but 0.1% Triton X-100 was too harsh, resulting in a complete loss of envelope during subsequent steps of immunogold labeling (data not shown). .. More interestingly, surface protrusions were visible in the outer surface of the envelope after the Tween 20 treatment, possibly indicating that as the envelope structure becomes loosely packed, some inner structures and binding sites for both VP26 and VP28 are well exposed, giving high labeling efficiency.

Two-Dimensional Gel Electrophoresis:

Article Title: A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts
Article Snippet: .. Two-dimensional electrophoresis Samples containing 75–200 μg of fractionated cytoplasmic proteins were diluted in 2D electrophoresis buffer (2 M thiourea, 5 M urea, 0.25% CHAPS, 0.25% Tween-20, 0.25% SB-3, 10% isopropanol, and 12.5% water-saturated butanol) containing 15 mg DTT and 0.5% ampholytes (pI ranges 3–10 or 5–8; Bio-Rad; Hercules, CA) to a final volume of 0.2 ml, and passively absorbed onto immobilized pH gradient strips (pI ranges 5–8 or 3–10 NL (non-linear); Bio-Rad) for 4 hours at 20°C followed by active rehydration (50 V at 20°C) for 8 hours. .. Isoelectric focusing was performed for 35,000 V-h on a Protean IEF System (Bio-Rad), electrophoresis with the Criterion gel system (Bio-Rad).

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  • ttb  (Bio-Rad)
    90
    Bio-Rad ttb
    Effects of reusing gel EB and fresh <t>TTB</t> on Western blots. A 25 μg CFBE-wt cell lysate was electrophoresed on individual, 4–20% Mini-PROTEAN TGX gels, transferred to a <t>PVDF</t> membrane, and subjected to Western blotting. Fresh SDS-PAGE buffer
    Ttb, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ttb/product/Bio-Rad
    Average 90 stars, based on 260 article reviews
    Price from $9.99 to $1999.99
    ttb - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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    Effects of reusing gel EB and fresh TTB on Western blots. A 25 μg CFBE-wt cell lysate was electrophoresed on individual, 4–20% Mini-PROTEAN TGX gels, transferred to a PVDF membrane, and subjected to Western blotting. Fresh SDS-PAGE buffer

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Effects of Reusing Gel Electrophoresis and Electrotransfer Buffers on Western Blotting

    doi: 10.7171/jbt.16-2703-004

    Figure Lengend Snippet: Effects of reusing gel EB and fresh TTB on Western blots. A 25 μg CFBE-wt cell lysate was electrophoresed on individual, 4–20% Mini-PROTEAN TGX gels, transferred to a PVDF membrane, and subjected to Western blotting. Fresh SDS-PAGE buffer

    Article Snippet: Following electrophoresis, gels were transferred to a PVDF membrane with TTB (25 mM Tris, 192 mM glycine, 20% methanol, 0.01% SDS, pH 8.3) using the Mini Trans-Blot electrophoretic transfer cell (Bio-Rad Laboratories).

    Techniques: Western Blot, SDS Page

    Effects of reusing gel EB and TTB on Western blots. A 25 μg CFBE-wt cell lysate was electrophoresed on individual, 4–20% Mini-PROTEAN TGX gels, as described in , transferred to PVDF membrane, and subjected to Western blotting. Fresh

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Effects of Reusing Gel Electrophoresis and Electrotransfer Buffers on Western Blotting

    doi: 10.7171/jbt.16-2703-004

    Figure Lengend Snippet: Effects of reusing gel EB and TTB on Western blots. A 25 μg CFBE-wt cell lysate was electrophoresed on individual, 4–20% Mini-PROTEAN TGX gels, as described in , transferred to PVDF membrane, and subjected to Western blotting. Fresh

    Article Snippet: Following electrophoresis, gels were transferred to a PVDF membrane with TTB (25 mM Tris, 192 mM glycine, 20% methanol, 0.01% SDS, pH 8.3) using the Mini Trans-Blot electrophoretic transfer cell (Bio-Rad Laboratories).

    Techniques: Western Blot