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Agrisera ttbs
Ttbs, supplied by Agrisera, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ttbs/product/Agrisera
Average 92 stars, based on 10 article reviews
Price from $9.99 to $1999.99
ttbs - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Electrophoresis:

Article Title: Desiccation tolerance in the Antarctic moss Sanionia uncinata
Article Snippet: .. Electrophoresis was performed for 2 h at 120 V. The product was transferred to a nitrocellulose membrane (Merck Millipore Ltda, Tullagreen, USA) using a 300 A for 1 h. The membrane was incubated for 1 h with 5% of fat free milk prepared in TTBS, the membrane was rinsed 3 times with TTBS and were incubated for 1 h at room temperature with the primary antibody anti-DHN 1:1000 prepared in 3% of fat free milk in TTBS (Agrisera, Sweden) o were incubated also for 1 h with the primary antibody anti-RbcL 1:20,000, after the incubation the membrane was rinsed for 15, 10, 5 min with TTBS and the membranes were incubated with the secondary antibody anti-Rabbit IgG HRP conjugated (Agrisera, Sweden) de 1:10,000, after antibody treatment, the membranes were incubated for 3 min with Luminata Forte substrate (Merck, Tullagreen, USA) and the chemiluminescence were detected with x-ray films (Fujifilm). .. Analysis of dhnA, gols, badh, p5cs and rrna18S gene transcript levels Total RNA was extracted per the manufacturer instructions described in the ISOLATE II RNA plant kit (Bioline reagents Ltda, London, United Kingdom), the purity was determined using the nanoquant assay (Infinite M200pro, Tecan, Sweden).

Western Blot:

Article Title: Isolation and Characterization of Copper- and Zinc- Binding Metallothioneins from the Marine Alga Ulva compressa (Chlorophyta)
Article Snippet: .. The membrane was incubated in TTBS containing 3% skim milk and the secondary antibody prepared against rabbit IgG coupled to hoseradish peroxidase (Agrisera, Vännas, Sweden) diluted 2000 times, for 1 h, and washed four times with TTBS for 15 min. Proteins were detected using ECL Western Blotting System kit (Amersham, Buckinghamshire, UK) and revealed using a C-Digits chemiluminiscence Western blot scanner Li-Cor (Lincoln, NE, USA) and Image Studio Digits software version 4.0 Li-Cor. .. Quantification of Copper and Zinc in Bacteria Expressing UcMTs-GST Recombinant bacteria were cultured in 100 mL of LB medium containing 100 mg mL−1 of carbenecillin until DO600 = 0.6, with 0.5 mM IPTG for 30 min, and with 1 mM CuSO4 or 1 mM ZnCl2 and IPTG for 6 h. Bacterial pellets showed a weight of 26–42 mg for copper cultures and 46–62 mg for zinc cultures.

Incubation:

Article Title: Elucidating the hypoxic stress response in barley (Hordeum vulgare L.) during waterlogging: A proteomics approach
Article Snippet: .. After being washed three times with TTBS, the blot was incubated in HRP-conjugated secondary antibodies (Agrisera, Sweden) diluted to 1:10 000 for 1 h at RT with agitation. .. After being washed, the immunoblot signals were detected using ECL (GE healthcare) and visualized on X - ray films (Fuji Medical X - ray film, FUJIFILM Corporation, Tokyo, Japan).

Article Title: Oligo-carrageenan kappa increases glucose, trehalose and TOR-P and subsequently stimulates the expression of genes involved in photosynthesis, and basal and secondary metabolisms in Eucalyptus globulus
Article Snippet: .. The membrane was incubated with the monoclonal antibody anti-human TOR-P Ser2448 which binds to Ser2424 in Arabidopsis TOR (1:1000, Abcam ab109268) or anti-RbcL (1:2500, Agrisera AS03037) (Schepetelnikov et al., 2013) at room temperature for 1 h. The membrane was washed three times with TTBS at room temperature for 10 min, incubated with the secondary antibody anti-Rabbit IgG conjugated with HRP (Agrisera, AS09602) at room temperature for 1 h, and washed three times with TTBS at room temperature for 10 min. .. The membrane was incubated with a chemo-luminescent substrate (SuperSignal West Femto, Thermo Scientific, Rockford, IL, USA) for 5 min and was exposed to an X-ray film (Thermo Scientific, Rockford, IL, USA) for 3 min to detect TOR-P ser2448, or for 30 s to detect RbcL.

Article Title: Desiccation tolerance in the Antarctic moss Sanionia uncinata
Article Snippet: .. Electrophoresis was performed for 2 h at 120 V. The product was transferred to a nitrocellulose membrane (Merck Millipore Ltda, Tullagreen, USA) using a 300 A for 1 h. The membrane was incubated for 1 h with 5% of fat free milk prepared in TTBS, the membrane was rinsed 3 times with TTBS and were incubated for 1 h at room temperature with the primary antibody anti-DHN 1:1000 prepared in 3% of fat free milk in TTBS (Agrisera, Sweden) o were incubated also for 1 h with the primary antibody anti-RbcL 1:20,000, after the incubation the membrane was rinsed for 15, 10, 5 min with TTBS and the membranes were incubated with the secondary antibody anti-Rabbit IgG HRP conjugated (Agrisera, Sweden) de 1:10,000, after antibody treatment, the membranes were incubated for 3 min with Luminata Forte substrate (Merck, Tullagreen, USA) and the chemiluminescence were detected with x-ray films (Fujifilm). .. Analysis of dhnA, gols, badh, p5cs and rrna18S gene transcript levels Total RNA was extracted per the manufacturer instructions described in the ISOLATE II RNA plant kit (Bioline reagents Ltda, London, United Kingdom), the purity was determined using the nanoquant assay (Infinite M200pro, Tecan, Sweden).

Article Title: Isolation and Characterization of Copper- and Zinc- Binding Metallothioneins from the Marine Alga Ulva compressa (Chlorophyta)
Article Snippet: .. The membrane was incubated in TTBS containing 3% skim milk and the secondary antibody prepared against rabbit IgG coupled to hoseradish peroxidase (Agrisera, Vännas, Sweden) diluted 2000 times, for 1 h, and washed four times with TTBS for 15 min. Proteins were detected using ECL Western Blotting System kit (Amersham, Buckinghamshire, UK) and revealed using a C-Digits chemiluminiscence Western blot scanner Li-Cor (Lincoln, NE, USA) and Image Studio Digits software version 4.0 Li-Cor. .. Quantification of Copper and Zinc in Bacteria Expressing UcMTs-GST Recombinant bacteria were cultured in 100 mL of LB medium containing 100 mg mL−1 of carbenecillin until DO600 = 0.6, with 0.5 mM IPTG for 30 min, and with 1 mM CuSO4 or 1 mM ZnCl2 and IPTG for 6 h. Bacterial pellets showed a weight of 26–42 mg for copper cultures and 46–62 mg for zinc cultures.

Software:

Article Title: Isolation and Characterization of Copper- and Zinc- Binding Metallothioneins from the Marine Alga Ulva compressa (Chlorophyta)
Article Snippet: .. The membrane was incubated in TTBS containing 3% skim milk and the secondary antibody prepared against rabbit IgG coupled to hoseradish peroxidase (Agrisera, Vännas, Sweden) diluted 2000 times, for 1 h, and washed four times with TTBS for 15 min. Proteins were detected using ECL Western Blotting System kit (Amersham, Buckinghamshire, UK) and revealed using a C-Digits chemiluminiscence Western blot scanner Li-Cor (Lincoln, NE, USA) and Image Studio Digits software version 4.0 Li-Cor. .. Quantification of Copper and Zinc in Bacteria Expressing UcMTs-GST Recombinant bacteria were cultured in 100 mL of LB medium containing 100 mg mL−1 of carbenecillin until DO600 = 0.6, with 0.5 mM IPTG for 30 min, and with 1 mM CuSO4 or 1 mM ZnCl2 and IPTG for 6 h. Bacterial pellets showed a weight of 26–42 mg for copper cultures and 46–62 mg for zinc cultures.

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    Agrisera ttbs
    Ttbs, supplied by Agrisera, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ttbs/product/Agrisera
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    ttbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Agrisera ttbs buffer
    Ttbs Buffer, supplied by Agrisera, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ttbs buffer/product/Agrisera
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ttbs buffer - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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