Journal: Disease Models & Mechanisms
Article Title: Liver-specific insulin receptor isoform A expression enhances hepatic glucose uptake and ameliorates liver steatosis in a mouse model of diet-induced obesity
Figure Lengend Snippet: Hepatic IRA expression ameliorates insulin tolerance. (A) Scheme of diet and AAV administration. The mouse groups were established as follows: STD ( n =5), HFD ( n =9), HFD-GFP ( n =6), HFD-IRA ( n =8) and HFD-IRB ( n =7). (B) Western blot analysis of IR in liver homogenates from the five groups studied (top). β-actin was used as a loading control. Histogram showing the IRβ/β-actin ratio quantification of band intensities (bottom). (C) GTT in STD ( n =5), HFD ( n =9), HFD-GFP ( n =9), HFD-IRA ( n =9) and HFD-IRB ( n =9) animals at 21 weeks after AAV administration. (D) ITT in STD ( n =5), HFD ( n =9), HFD-GFP ( n =9), HFD-IRA ( n =9) and HFD-IRB ( n =9) animals at 21 weeks after AAV administration. Results are expressed as mean±s.e.m. Statistical significance was assessed by one-way ANOVA with Bonferroni post test in B, and two-tailed unpaired Student's t -test in C and D [versus STD mice (* P
Article Snippet: The antibodies used were anti-IRβ (sc-711; 1:2000 in TTBS) from Santa Cruz Biotechnology (Dallas, TX, USA) and anti-β-actin (A2228; 1:5000 in TTBS) from Sigma-Aldrich (St. Louis, MO, USA).
Techniques: Expressing, Western Blot, Two Tailed Test, Mouse Assay